AlfalfaGEDB | MS.gene30945

M. sativa cultivar XinJiangDaYe / MS.gene30945

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30945.t1	XP_003597343.1	92.8	153	3	1	1	145	1	153	7.80E-67	263.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30945.t1	Q2HU65	92.8	153	3	1	1	145	1	153	1.9e-69	263.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30945.t1	G7IHN1	92.8	153	3	1	1	145	1	153	5.6e-67	263.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene30945	0.821623	3.66E-53	-1.69E-46
MS.gene049850	MS.gene30945	0.803194	3.92E-49	-1.69E-46
MS.gene050807	MS.gene30945	0.82208	2.87E-53	-1.69E-46
MS.gene05157	MS.gene30945	0.806043	9.96E-50	-1.69E-46
MS.gene051720	MS.gene30945	0.802229	6.19E-49	-1.69E-46
MS.gene052133	MS.gene30945	0.83526	1.86E-56	-1.69E-46
MS.gene053581	MS.gene30945	0.83238	9.77E-56	-1.69E-46
MS.gene05462	MS.gene30945	0.80876	2.64E-50	-1.69E-46
MS.gene055466	MS.gene30945	0.806044	9.96E-50	-1.69E-46
MS.gene05608	MS.gene30945	0.815944	7.13E-52	-1.69E-46
MS.gene057915	MS.gene30945	0.808257	3.38E-50	-1.69E-46
MS.gene058216	MS.gene30945	0.809507	1.83E-50	-1.69E-46
MS.gene05978	MS.gene30945	0.8105	1.12E-50	-1.69E-46
MS.gene060587	MS.gene30945	0.811482	6.85E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30945.t1	MTR_2g096570	92.810	153	3	1	1	145	1	153	2.03e-94	269
MS.gene30945.t1	MTR_4g063280	87.407	135	6	3	19	145	14	145	2.57e-74	218
MS.gene30945.t1	MTR_4g071150	87.013	154	9	3	1	145	1	152	8.57e-73	214
MS.gene30945.t1	MTR_2g096610	83.660	153	12	3	1	145	1	148	2.13e-71	211
MS.gene30945.t1	MTR_4g063410	85.714	154	7	4	1	145	1	148	7.48e-69	204
MS.gene30945.t1	MTR_4g064005	85.065	154	8	4	1	145	1	148	1.09e-68	204
MS.gene30945.t1	MTR_4g064967	85.065	154	8	4	1	145	1	148	5.62e-68	202
MS.gene30945.t1	MTR_8g086640	91.667	132	2	2	1	123	1	132	2.68e-65	196
MS.gene30945.t1	MTR_7g113160	75.188	133	21	3	9	130	13	144	3.62e-62	187
MS.gene30945.t1	MTR_7g093910	66.406	128	32	2	2	121	4	128	4.57e-51	159
MS.gene30945.t1	MTR_7g108320	68.750	128	26	4	3	121	6	128	5.10e-51	159
MS.gene30945.t1	MTR_7g114060	67.188	128	29	3	3	121	2	125	2.12e-50	157
MS.gene30945.t1	MTR_7g114040	67.188	128	29	3	3	121	2	125	2.12e-50	157
MS.gene30945.t1	MTR_2g082510	68.067	119	30	1	10	120	3	121	9.40e-50	155
MS.gene30945.t1	MTR_2g082220	67.227	119	31	1	10	120	3	121	1.14e-48	153
MS.gene30945.t1	MTR_6g005150	68.421	114	30	2	23	130	26	139	2.03e-48	152
MS.gene30945.t1	MTR_7g114050	65.789	114	24	3	16	121	10	116	4.12e-41	134
MS.gene30945.t1	MTR_4g063300	68.807	109	17	3	44	144	10	109	5.18e-37	123
MS.gene30945.t1	MTR_1g011750	54.464	112	41	3	20	122	23	133	1.49e-27	99.8
MS.gene30945.t1	MTR_1g011850	54.464	112	41	3	20	122	23	133	1.49e-27	99.8
MS.gene30945.t1	MTR_1g114050	55.046	109	39	3	20	119	20	127	2.33e-27	99.0
MS.gene30945.t1	MTR_1g071040	55.769	104	36	3	25	119	28	130	4.53e-26	95.9
MS.gene30945.t1	MTR_1g069770	60.811	74	28	1	58	130	31	104	2.12e-25	93.2
MS.gene30945.t1	MTR_7g010440	70.588	51	15	0	69	119	122	172	1.27e-16	74.7
MS.gene30945.t1	MTR_4g005910	74.419	43	11	0	78	120	4	46	2.52e-16	68.9
MS.gene30945.t1	MTR_4g048490	45.361	97	32	3	23	119	26	101	5.91e-14	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30945.t1	AT5G02560	75.325	154	28	3	1	145	1	153	2.59e-69	206
MS.gene30945.t1	AT5G02560	65.169	178	28	3	1	145	1	177	5.02e-66	198
MS.gene30945.t1	AT5G27670	69.737	152	32	3	2	145	5	150	2.51e-64	193
MS.gene30945.t1	AT5G59870	68.182	154	36	3	1	145	1	150	1.24e-63	191
MS.gene30945.t1	AT1G51060	70.000	120	28	1	10	121	3	122	3.88e-52	162
MS.gene30945.t1	AT4G27230	68.908	119	29	1	10	120	3	121	8.69e-51	158
MS.gene30945.t1	AT4G27230	68.908	119	29	1	10	120	3	121	8.69e-51	158
MS.gene30945.t1	AT3G20670	65.116	129	37	1	10	130	3	131	2.01e-50	157
MS.gene30945.t1	AT5G54640	68.067	119	30	1	10	120	3	121	2.17e-49	155
MS.gene30945.t1	AT1G08880	65.873	126	32	2	7	121	3	128	2.45e-49	155
MS.gene30945.t1	AT1G54690	66.667	123	30	2	10	121	6	128	7.01e-49	154
MS.gene30945.t1	AT2G38810	54.464	112	41	3	20	122	25	135	3.56e-28	101
MS.gene30945.t1	AT2G38810	54.464	112	41	3	20	122	25	135	3.56e-28	101
MS.gene30945.t1	AT2G38810	54.464	112	41	3	20	122	25	135	3.56e-28	101
MS.gene30945.t1	AT2G38810	54.464	112	41	3	20	122	25	135	3.56e-28	101
MS.gene30945.t1	AT1G52740	56.190	105	36	3	24	119	27	130	1.64e-26	97.1
MS.gene30945.t1	AT3G54560	53.704	108	40	3	24	122	29	135	3.22e-26	96.3
MS.gene30945.t1	AT3G54560	53.704	108	40	3	24	122	29	135	3.22e-26	96.3
MS.gene30945.t1	AT4G13570	42.391	92	43	3	31	113	28	118	1.14e-12	61.2
MS.gene30945.t1	AT4G13570	41.758	91	43	3	32	113	35	124	2.46e-12	60.5

Find 46 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTAATCTAAGCCTTCTT+TGG	0.260442	2.2:-5497995	None:intergenic
GTAGACCTTTCAGTCTTCTT+AGG	0.271890	2.2:-5497923	None:intergenic
GTCTTCTTAGGCAAAAGAAC+AGG	0.334824	2.2:-5497911	None:intergenic
ACCAGCTCCTTTCTTCGTCT+TGG	0.348361	2.2:-5497468	None:intergenic
AGAACAGCGGCGAGGTAAAC+AGG	0.381456	2.2:-5497623	None:intergenic
AAAAGAACAGGGTTAATGTT+TGG	0.382061	2.2:-5497899	None:intergenic
CAATTTCCAGTCGGAAGAAT+CGG	0.394685	2.2:+5497551	MS.gene30945:CDS
GCCTTAGGCTTAGGAGACTT+TGG	0.397268	2.2:-5497962	None:intergenic
GACTTTCCAGCCTTAGGCTT+AGG	0.400283	2.2:-5497971	None:intergenic
CTGGTCACAGACTTCTTCCT+TGG	0.400754	2.2:-5497509	None:intergenic
TTTGGAGACTTTCCAGCCTT+AGG	0.406671	2.2:-5497977	None:intergenic
GTATGTTTAGGTTCTTGAAT+TGG	0.426207	2.2:+5497748	MS.gene30945:intron
GTTTAGGTTCTTGAATTGGC+TGG	0.434556	2.2:+5497752	MS.gene30945:intron
AAAGGAGCTGGTGGAAGGAA+AGG	0.477487	2.2:+5497479	MS.gene30945:CDS
AATTCCAAGGCACGTGTTGT+TGG	0.477559	2.2:+5497808	MS.gene30945:CDS
GGAGCTGGTGGAAGGAAAGG+TGG	0.481033	2.2:+5497482	MS.gene30945:CDS
GCAAGCACCAAGACGAAGAA+AGG	0.482883	2.2:+5497461	MS.gene30945:CDS
ACAGCCAACAACACGTGCCT+TGG	0.485674	2.2:-5497812	None:intergenic
AAGACCAGCTCTGATGGATC+TGG	0.491289	2.2:-5497528	None:intergenic
AAATTGAAGACCAGCTCTGA+TGG	0.498711	2.2:-5497534	None:intergenic
TATGCTCAGCGTGTTGGTAC+TGG	0.513208	2.2:+5497596	MS.gene30945:CDS
CACGTGATAACAAGAAGAAT+AGG	0.529318	2.2:+5497783	MS.gene30945:CDS
GGCTGGAAAGTCTCCAAAGA+AGG	0.542007	2.2:+5497982	MS.gene30945:CDS
AAGTCTCCTAAGCCTAAGGC+TGG	0.542736	2.2:+5497965	MS.gene30945:CDS
ATCTACCTTTCTTCAAGTAA+CGG	0.547675	2.2:-5497574	None:intergenic
GGTAGATATGCTCAGCGTGT+TGG	0.549879	2.2:+5497590	MS.gene30945:CDS
ACCAAAGTCTCCTAAGCCTA+AGG	0.550123	2.2:+5497961	MS.gene30945:CDS
TGAAGAGCTAGGAAAATTGT+TGG	0.550899	2.2:+5497844	MS.gene30945:CDS
GTGACCAGATCCATCAGAGC+TGG	0.553138	2.2:+5497524	MS.gene30945:CDS
ATCGGCCGTTACTTGAAGAA+AGG	0.553177	2.2:+5497569	MS.gene30945:CDS
CGAAGAAAGGAGCTGGTGGA+AGG	0.557443	2.2:+5497474	MS.gene30945:CDS
GTGAGGAATGATGAAGAGCT+AGG	0.566133	2.2:+5497833	MS.gene30945:CDS
AAGGTCTACTAATGCTAGCA+AGG	0.572544	2.2:+5497937	MS.gene30945:CDS
TAACGGCCGATTCTTCCGAC+TGG	0.578466	2.2:-5497557	None:intergenic
ACCAAGACGAAGAAAGGAGC+TGG	0.579581	2.2:+5497467	MS.gene30945:CDS
GAGCTAGGAAAATTGTTGGC+TGG	0.600802	2.2:+5497848	MS.gene30945:CDS
GAAGGAAAGGTGGAGGACCA+AGG	0.610388	2.2:+5497492	MS.gene30945:CDS
TCTTCTTAGGCAAAAGAACA+GGG	0.618533	2.2:-5497910	None:intergenic
GCTGGTGGAAGGAAAGGTGG+AGG	0.624747	2.2:+5497485	MS.gene30945:CDS
AGAAGAATAGGATAATTCCA+AGG	0.643694	2.2:+5497795	MS.gene30945:CDS
GCTGGTCTTCAATTTCCAGT+CGG	0.654384	2.2:+5497542	MS.gene30945:CDS
AAGACGAAGAAAGGAGCTGG+TGG	0.662071	2.2:+5497470	MS.gene30945:CDS
GGCACGTGTTGTTGGCTGTG+AGG	0.669170	2.2:+5497816	MS.gene30945:CDS
GATATTCAAGAACAGCGGCG+AGG	0.724749	2.2:-5497631	None:intergenic
GCTGGTGTAACAATTGCACA+TGG	0.735207	2.2:+5497866	MS.gene30945:CDS
GGTGTAACAATTGCACATGG+TGG	0.749624	2.2:+5497869	MS.gene30945:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTAAACATACAATAAAATTA+GGG	-	chr2.2:5497738-5497757	None:intergenic	15.0%
!!	AATCAAAGATCATAAGAAAA+TGG	-	chr2.2:5497682-5497701	None:intergenic	20.0%
!!	CCTAAACATACAATAAAATT+AGG	-	chr2.2:5497739-5497758	None:intergenic	20.0%
!!!	CCTAATTTTATTGTATGTTT+AGG	+	chr2.2:5497736-5497755	MS.gene30945:intron	20.0%
!	AGGGTTACAAATTCAAAATT+AGG	-	chr2.2:5497719-5497738	None:intergenic	25.0%
!	GGGTTACAAATTCAAAATTA+GGG	-	chr2.2:5497718-5497737	None:intergenic	25.0%
	AAAAGAACAGGGTTAATGTT+TGG	-	chr2.2:5497902-5497921	None:intergenic	30.0%
	AGAAGAATAGGATAATTCCA+AGG	+	chr2.2:5497795-5497814	MS.gene30945:CDS	30.0%
	ATCTACCTTTCTTCAAGTAA+CGG	-	chr2.2:5497577-5497596	None:intergenic	30.0%
!	GTATGTTTAGGTTCTTGAAT+TGG	+	chr2.2:5497748-5497767	MS.gene30945:intron	30.0%
	AGCAAGATATTCAAGAACAG+CGG	-	chr2.2:5497639-5497658	None:intergenic	35.0%
	CACGTGATAACAAGAAGAAT+AGG	+	chr2.2:5497783-5497802	MS.gene30945:CDS	35.0%
	TCTTCTTAGGCAAAAGAACA+GGG	-	chr2.2:5497913-5497932	None:intergenic	35.0%
	TGAAGAGCTAGGAAAATTGT+TGG	+	chr2.2:5497844-5497863	MS.gene30945:CDS	35.0%
!	TTTTGCCTAAGAAGACTGAA+AGG	+	chr2.2:5497918-5497937	MS.gene30945:CDS	35.0%
	AAGGTCTACTAATGCTAGCA+AGG	+	chr2.2:5497937-5497956	MS.gene30945:CDS	40.0%
	CAATTTCCAGTCGGAAGAAT+CGG	+	chr2.2:5497551-5497570	MS.gene30945:CDS	40.0%
	GTAGACCTTTCAGTCTTCTT+AGG	-	chr2.2:5497926-5497945	None:intergenic	40.0%
	GTCTTCTTAGGCAAAAGAAC+AGG	-	chr2.2:5497914-5497933	None:intergenic	40.0%
	TCTTGAATATCTTGCTGCTG+AGG	+	chr2.2:5497643-5497662	MS.gene30945:intron	40.0%
!	AAATTGAAGACCAGCTCTGA+TGG	-	chr2.2:5497537-5497556	None:intergenic	40.0%
!	GTTTAGGTTCTTGAATTGGC+TGG	+	chr2.2:5497752-5497771	MS.gene30945:intron	40.0%
	AATTCCAAGGCACGTGTTGT+TGG	+	chr2.2:5497808-5497827	MS.gene30945:CDS	45.0%
	ACCAAAGTCTCCTAAGCCTA+AGG	+	chr2.2:5497961-5497980	MS.gene30945:CDS	45.0%
	ATCGGCCGTTACTTGAAGAA+AGG	+	chr2.2:5497569-5497588	MS.gene30945:CDS	45.0%
	GAGCTAGGAAAATTGTTGGC+TGG	+	chr2.2:5497848-5497867	MS.gene30945:CDS	45.0%
	GCTGGTGTAACAATTGCACA+TGG	+	chr2.2:5497866-5497885	MS.gene30945:CDS	45.0%
	GGTGTAACAATTGCACATGG+TGG	+	chr2.2:5497869-5497888	MS.gene30945:CDS	45.0%
	GTGAGGAATGATGAAGAGCT+AGG	+	chr2.2:5497833-5497852	MS.gene30945:CDS	45.0%
	TTTGGAGACTTTCCAGCCTT+AGG	-	chr2.2:5497980-5497999	None:intergenic	45.0%
!!	GCTGGTCTTCAATTTCCAGT+CGG	+	chr2.2:5497542-5497561	MS.gene30945:CDS	45.0%
	AAAGGAGCTGGTGGAAGGAA+AGG	+	chr2.2:5497479-5497498	MS.gene30945:CDS	50.0%
	AAGACGAAGAAAGGAGCTGG+TGG	+	chr2.2:5497470-5497489	MS.gene30945:CDS	50.0%
	AAGTCTCCTAAGCCTAAGGC+TGG	+	chr2.2:5497965-5497984	MS.gene30945:CDS	50.0%
	ACCAAGACGAAGAAAGGAGC+TGG	+	chr2.2:5497467-5497486	MS.gene30945:CDS	50.0%
	ACCAGCTCCTTTCTTCGTCT+TGG	-	chr2.2:5497471-5497490	None:intergenic	50.0%
	CTGGTCACAGACTTCTTCCT+TGG	-	chr2.2:5497512-5497531	None:intergenic	50.0%
	GACTTTCCAGCCTTAGGCTT+AGG	-	chr2.2:5497974-5497993	None:intergenic	50.0%
	GATATTCAAGAACAGCGGCG+AGG	-	chr2.2:5497634-5497653	None:intergenic	50.0%
	GCAAGCACCAAGACGAAGAA+AGG	+	chr2.2:5497461-5497480	MS.gene30945:CDS	50.0%
	GCCTTAGGCTTAGGAGACTT+TGG	-	chr2.2:5497965-5497984	None:intergenic	50.0%
	GGCTGGAAAGTCTCCAAAGA+AGG	+	chr2.2:5497982-5498001	MS.gene30945:CDS	50.0%
	GGTAGATATGCTCAGCGTGT+TGG	+	chr2.2:5497590-5497609	MS.gene30945:CDS	50.0%
!	AAGACCAGCTCTGATGGATC+TGG	-	chr2.2:5497531-5497550	None:intergenic	50.0%
!	TATGCTCAGCGTGTTGGTAC+TGG	+	chr2.2:5497596-5497615	MS.gene30945:CDS	50.0%
	ACAGCCAACAACACGTGCCT+TGG	-	chr2.2:5497815-5497834	None:intergenic	55.0%
	AGAACAGCGGCGAGGTAAAC+AGG	-	chr2.2:5497626-5497645	None:intergenic	55.0%
	CGAAGAAAGGAGCTGGTGGA+AGG	+	chr2.2:5497474-5497493	MS.gene30945:CDS	55.0%
	GAAGGAAAGGTGGAGGACCA+AGG	+	chr2.2:5497492-5497511	MS.gene30945:CDS	55.0%
	GTGACCAGATCCATCAGAGC+TGG	+	chr2.2:5497524-5497543	MS.gene30945:CDS	55.0%
!	TAACGGCCGATTCTTCCGAC+TGG	-	chr2.2:5497560-5497579	None:intergenic	55.0%
	GCTGGTGGAAGGAAAGGTGG+AGG	+	chr2.2:5497485-5497504	MS.gene30945:CDS	60.0%
	GGAGCTGGTGGAAGGAAAGG+TGG	+	chr2.2:5497482-5497501	MS.gene30945:CDS	60.0%
!	GGCACGTGTTGTTGGCTGTG+AGG	+	chr2.2:5497816-5497835	MS.gene30945:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	5497455	5498009	5497455	ID=MS.gene30945
chr2.2	mRNA	5497455	5498009	5497455	ID=MS.gene30945.t1;Parent=MS.gene30945
chr2.2	exon	5497455	5497640	5497455	ID=MS.gene30945.t1.exon1;Parent=MS.gene30945.t1
chr2.2	CDS	5497455	5497640	5497455	ID=cds.MS.gene30945.t1;Parent=MS.gene30945.t1
chr2.2	exon	5497758	5498009	5497758	ID=MS.gene30945.t1.exon2;Parent=MS.gene30945.t1
chr2.2	CDS	5497758	5498009	5497758	ID=cds.MS.gene30945.t1;Parent=MS.gene30945.t1

Gene Sequence

>MS.gene30945

ATGGACGCAAGCACCAAGACGAAGAAAGGAGCTGGTGGAAGGAAAGGTGGAGGACCAAGGAAGAAGTCTGTGACCAGATCCATCAGAGCTGGTCTTCAATTTCCAGTCGGAAGAATCGGCCGTTACTTGAAGAAAGGTAGATATGCTCAGCGTGTTGGTACTGGTGCTCCTGTTTACCTCGCCGCTGTTCTTGAATATCTTGCTGCTGAGGTGAATTTCAATTTCCATTTTCTTATGATCTTTGATTTTGAATTTTGAAACCCTAATTTTGAATTTGTAACCCTAATTTTATTGTATGTTTAGGTTCTTGAATTGGCTGGAAATGCTGCACGTGATAACAAGAAGAATAGGATAATTCCAAGGCACGTGTTGTTGGCTGTGAGGAATGATGAAGAGCTAGGAAAATTGTTGGCTGGTGTAACAATTGCACATGGTGGTGTTCTTCCAAACATTAACCCTGTTCTTTTGCCTAAGAAGACTGAAAGGTCTACTAATGCTAGCAAGGAACCAAAGTCTCCTAAGCCTAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene30945.t1

MDASTKTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPVLLPKKTERSTNASKEPKSPKPKAGKSPKKA