AlfalfaGEDB | MS.gene05608

M. sativa cultivar XinJiangDaYe / MS.gene05608

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05608.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05608.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05608.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050901	MS.gene05608	0.822904	1.85E-53	-1.69E-46
MS.gene053581	MS.gene05608	0.816744	4.72E-52	-1.69E-46
MS.gene05608	MS.gene056215	0.838792	2.33E-57	-1.69E-46
MS.gene05608	MS.gene061866	0.836046	1.18E-56	-1.69E-46
MS.gene05608	MS.gene061869	0.842356	2.72E-58	-1.69E-46
MS.gene05608	MS.gene061872	0.849807	2.55E-60	-1.69E-46
MS.gene05608	MS.gene061874	0.844856	5.83E-59	-1.69E-46
MS.gene05608	MS.gene068022	0.819712	1.01E-52	-1.69E-46
MS.gene05608	MS.gene070241	0.802598	5.20E-49	-1.69E-46
MS.gene05608	MS.gene070242	0.838613	2.59E-57	-1.69E-46
MS.gene05608	MS.gene070943	0.805395	1.36E-49	-1.69E-46
MS.gene05608	MS.gene072493	0.835493	1.63E-56	-1.69E-46
MS.gene05608	MS.gene09873	0.815747	7.89E-52	-1.69E-46
MS.gene05608	MS.gene23112	0.824419	8.16E-54	-1.69E-46
MS.gene05608	MS.gene30945	0.815944	7.13E-52	-1.69E-46
MS.gene05608	MS.gene30946	0.839463	1.56E-57	-1.69E-46
MS.gene05608	MS.gene36265	0.801747	7.78E-49	-1.69E-46
MS.gene05608	MS.gene45478	0.800016	1.76E-48	-1.69E-46
MS.gene05608	MS.gene45479	0.809344	1.98E-50	-1.69E-46
MS.gene05608	MS.gene49359	0.815471	9.09E-52	-1.69E-46
MS.gene05608	MS.gene50564	0.833645	4.74E-56	-1.69E-46
MS.gene05608	MS.gene52594	0.825098	5.64E-54	-1.69E-46
MS.gene05608	MS.gene55623	0.802054	6.73E-49	-1.69E-46
MS.gene05608	MS.gene60093	0.809876	1.52E-50	-1.69E-46
MS.gene05608	MS.gene61113	0.801529	8.63E-49	-1.69E-46
MS.gene05608	MS.gene61739	0.805938	1.05E-49	-1.69E-46
MS.gene05608	MS.gene64650	0.812005	5.27E-51	-1.69E-46
MS.gene05608	MS.gene70955	0.82219	2.70E-53	-1.69E-46
MS.gene05608	MS.gene71215	0.804707	1.90E-49	-1.69E-46
MS.gene05608	MS.gene73136	0.807222	5.62E-50	-1.69E-46
MS.gene05608	MS.gene78578	0.835848	1.32E-56	-1.69E-46
MS.gene05608	MS.gene82546	0.837455	5.15E-57	-1.69E-46
MS.gene05608	MS.gene88680	0.860956	1.44E-63	-1.69E-46
MS.gene05608	MS.gene88681	0.800909	1.15E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene035718	MS.gene05608	PPI
MS.gene008276	MS.gene05608	PPI
MS.gene05608	MS.gene04052	PPI
MS.gene05608	MS.gene061866	PPI
MS.gene09873	MS.gene05608	PPI
MS.gene05608	MS.gene64650	PPI
MS.gene05608	MS.gene048705	PPI
MS.gene05608	MS.gene061874	PPI
MS.gene05608	MS.gene04576	PPI
MS.gene78578	MS.gene05608	PPI
MS.gene035721	MS.gene05608	PPI
MS.gene08122	MS.gene05608	PPI
MS.gene05608	MS.gene061869	PPI
MS.gene048705	MS.gene05608	PPI
MS.gene05623	MS.gene05608	PPI
MS.gene043967	MS.gene05608	PPI
MS.gene064557	MS.gene05608	PPI
MS.gene84536	MS.gene05608	PPI
MS.gene028629	MS.gene05608	PPI
MS.gene82546	MS.gene05608	PPI
MS.gene61017	MS.gene05608	PPI
MS.gene04433	MS.gene05608	PPI
MS.gene04452	MS.gene05608	PPI
MS.gene05608	MS.gene061867	PPI
MS.gene035787	MS.gene05608	PPI
MS.gene04067	MS.gene05608	PPI
MS.gene71215	MS.gene05608	PPI
MS.gene061866	MS.gene05608	PPI
MS.gene05608	MS.gene008276	PPI
MS.gene89487	MS.gene05608	PPI
MS.gene05608	MS.gene05619	PPI
MS.gene05608	MS.gene73136	PPI
MS.gene035719	MS.gene05608	PPI
MS.gene58854	MS.gene05608	PPI
MS.gene05608	MS.gene89488	PPI
MS.gene66225	MS.gene05608	PPI
MS.gene70955	MS.gene05608	PPI
MS.gene071136	MS.gene05608	PPI
MS.gene040383	MS.gene05608	PPI
MS.gene070242	MS.gene05608	PPI
MS.gene072493	MS.gene05608	PPI
MS.gene035720	MS.gene05608	PPI
MS.gene012390	MS.gene05608	PPI
MS.gene048746	MS.gene05608	PPI
MS.gene010220	MS.gene05608	PPI
MS.gene05608	MS.gene035720	PPI
MS.gene05608	MS.gene61017	PPI
MS.gene79195	MS.gene05608	PPI
MS.gene05608	MS.gene012390	PPI
MS.gene88680	MS.gene05608	PPI
MS.gene056215	MS.gene05608	PPI
MS.gene049821	MS.gene05608	PPI
MS.gene62520	MS.gene05608	PPI
MS.gene038469	MS.gene05608	PPI
MS.gene05608	MS.gene010219	PPI
MS.gene05608	MS.gene68545	PPI
MS.gene09871	MS.gene05608	PPI
MS.gene05608	MS.gene016624	PPI
MS.gene04576	MS.gene05608	PPI
MS.gene060463	MS.gene05608	PPI
MS.gene04392	MS.gene05608	PPI
MS.gene30920	MS.gene05608	PPI
MS.gene08053	MS.gene05608	PPI
MS.gene061872	MS.gene05608	PPI
MS.gene04068	MS.gene05608	PPI
MS.gene070241	MS.gene05608	PPI
MS.gene04434	MS.gene05608	PPI
MS.gene022946	MS.gene05608	PPI
MS.gene05608	MS.gene049705	PPI
MS.gene04064	MS.gene05608	PPI
MS.gene061876	MS.gene05608	PPI
MS.gene022932	MS.gene05608	PPI
MS.gene012391	MS.gene05608	PPI
MS.gene88681	MS.gene05608	PPI
MS.gene070244	MS.gene05608	PPI
MS.gene035897	MS.gene05608	PPI
MS.gene035896	MS.gene05608	PPI
MS.gene73136	MS.gene05608	PPI
MS.gene64920	MS.gene05608	PPI
MS.gene05608	MS.gene84536	PPI
MS.gene070907	MS.gene05608	PPI
MS.gene009027	MS.gene05608	PPI
MS.gene05608	MS.gene60093	PPI
MS.gene31196	MS.gene05608	PPI
MS.gene010219	MS.gene05608	PPI
MS.gene05608	MS.gene09873	PPI
MS.gene04052	MS.gene05608	PPI
MS.gene060465	MS.gene05608	PPI
MS.gene05608	MS.gene04972	PPI
MS.gene05608	MS.gene004121	PPI
MS.gene05608	MS.gene62520	PPI
MS.gene05608	MS.gene018578	PPI
MS.gene08121	MS.gene05608	PPI
MS.gene05608	MS.gene018580	PPI
MS.gene05608	MS.gene004124	PPI
MS.gene061870	MS.gene05608	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05608.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05608.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene05608.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05608.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05608.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.2:+53686736	MS.gene05608:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.431401	3.2:+53686845	MS.gene05608:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.438131	3.2:+53686851	MS.gene05608:CDS
CAATCGACGAATAGCAGGTT+TGG	0.439411	3.2:-53686660	None:intergenic
ATCAACCTCCGAAACCATAG+AGG	0.481091	3.2:-53686859	None:intergenic
CTTGCCAATCGACGAATAGC+AGG	0.487494	3.2:-53686665	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.2:+53686590	MS.gene05608:CDS
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.2:+53686574	MS.gene05608:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.2:+53686587	MS.gene05608:CDS
CTCGCGAAAATGTCAGGCCG+TGG	0.510433	3.2:+53686560	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.2:-53686732	None:intergenic
GTCCCTTACCTCCCTTTCCA+CGG	0.513750	3.2:-53686577	None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG	0.516096	3.2:+53686566	None:intergenic
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.2:+53686586	MS.gene05608:CDS
GGTTCTTCGTGATAACATCC+AGG	0.522451	3.2:+53686631	MS.gene05608:CDS
AGGTGCAAAGAGGCATAGAA+AGG	0.530485	3.2:+53686610	MS.gene05608:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.2:+53686581	MS.gene05608:CDS
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.2:+53686575	MS.gene05608:CDS
CAAACCTGCTATTCGTCGAT+TGG	0.553041	3.2:+53686661	MS.gene05608:CDS
GAAAATGTCAGGCCGTGGAA+AGG	0.558967	3.2:+53686565	None:intergenic
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.2:+53686830	MS.gene05608:CDS
TTACATACACTGAGCATGCA+AGG	0.586915	3.2:+53686780	MS.gene05608:CDS
GAGGAAAACTGTGACAGCCA+TGG	0.588212	3.2:+53686802	MS.gene05608:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.593946	3.2:+53686674	MS.gene05608:CDS
TAGCAGGTTTGGTGATACCC+TGG	0.596796	3.2:-53686649	None:intergenic
AATCAGTGGATTGATCTATG+AGG	0.599604	3.2:+53686706	MS.gene05608:CDS
GGTGGTGTCAAGAGAATCAG+TGG	0.607763	3.2:+53686692	MS.gene05608:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.629429	3.2:+53686671	MS.gene05608:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.2:-53686819	None:intergenic
AGAACCCTCTATGGTTTCGG+AGG	0.630900	3.2:+53686854	MS.gene05608:CDS
GTTCTTCGTGATAACATCCA+GGG	0.632029	3.2:+53686632	MS.gene05608:CDS
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.2:+53686569	MS.gene05608:CDS
TCAACCTCCGAAACCATAGA+GGG	0.666233	3.2:-53686858	None:intergenic
TCGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.2:+53686600	MS.gene05608:CDS
CAGTGTATGTAACTGCATCA+CGG	0.689582	3.2:-53686769	None:intergenic
TTGATCTATGAGGAAACCAG+AGG	0.700822	3.2:+53686716	MS.gene05608:CDS
CATACACTGAGCATGCAAGG+AGG	0.758591	3.2:+53686783	MS.gene05608:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATCAGTGGATTGATCTATG+AGG	+	chr3.2:53686706-53686725	MS.gene05608:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.2:53686736-53686755	MS.gene05608:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	-	chr3.2:53686822-53686841	None:intergenic	40.0%
	CAGTGTATGTAACTGCATCA+CGG	-	chr3.2:53686772-53686791	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	+	chr3.2:53686632-53686651	MS.gene05608:CDS	40.0%
	TTACATACACTGAGCATGCA+AGG	+	chr3.2:53686780-53686799	MS.gene05608:CDS	40.0%
	TTGATCTATGAGGAAACCAG+AGG	+	chr3.2:53686716-53686735	MS.gene05608:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	+	chr3.2:53686830-53686849	MS.gene05608:CDS	40.0%
	AGACAAGGAAGAACCCTCTA+TGG	+	chr3.2:53686845-53686864	MS.gene05608:CDS	45.0%
	CAAACCTGCTATTCGTCGAT+TGG	+	chr3.2:53686661-53686680	MS.gene05608:CDS	45.0%
	CAATCGACGAATAGCAGGTT+TGG	-	chr3.2:53686663-53686682	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	-	chr3.2:53686735-53686754	None:intergenic	45.0%
	GGAAGAACCCTCTATGGTTT+CGG	+	chr3.2:53686851-53686870	MS.gene05608:CDS	45.0%
	GGTTCTTCGTGATAACATCC+AGG	+	chr3.2:53686631-53686650	MS.gene05608:CDS	45.0%
	TCAACCTCCGAAACCATAGA+GGG	-	chr3.2:53686861-53686880	None:intergenic	45.0%
!	AGGTGCAAAGAGGCATAGAA+AGG	+	chr3.2:53686610-53686629	MS.gene05608:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	chr3.2:53686671-53686690	MS.gene05608:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+AGG	+	chr3.2:53686854-53686873	MS.gene05608:CDS	50.0%
	CATACACTGAGCATGCAAGG+AGG	+	chr3.2:53686783-53686802	MS.gene05608:CDS	50.0%
	CTTGCCAATCGACGAATAGC+AGG	-	chr3.2:53686668-53686687	None:intergenic	50.0%
	GAGGAAAACTGTGACAGCCA+TGG	+	chr3.2:53686802-53686821	MS.gene05608:CDS	50.0%
!	GGTGGTGTCAAGAGAATCAG+TGG	+	chr3.2:53686692-53686711	MS.gene05608:CDS	50.0%
!!	TAGCAGGTTTGGTGATACCC+TGG	-	chr3.2:53686652-53686671	None:intergenic	50.0%
	GGAAAGGGAGGTAAGGGACT+CGG	+	chr3.2:53686581-53686600	MS.gene05608:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	+	chr3.2:53686587-53686606	MS.gene05608:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	-	chr3.2:53686580-53686599	None:intergenic	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	chr3.2:53686674-53686693	MS.gene05608:CDS	55.0%
!	TCGGAAAGGGAGGTGCAAAG+AGG	+	chr3.2:53686600-53686619	MS.gene05608:CDS	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	+	chr3.2:53686574-53686593	MS.gene05608:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	+	chr3.2:53686569-53686588	MS.gene05608:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	+	chr3.2:53686575-53686594	MS.gene05608:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	+	chr3.2:53686586-53686605	MS.gene05608:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	+	chr3.2:53686590-53686609	MS.gene05608:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	53686569	53686880	53686569	ID=MS.gene05608
chr3.2	mRNA	53686569	53686880	53686569	ID=MS.gene05608.t1;Parent=MS.gene05608
chr3.2	exon	53686569	53686880	53686569	ID=MS.gene05608.t1.exon1;Parent=MS.gene05608.t1
chr3.2	CDS	53686569	53686880	53686569	ID=cds.MS.gene05608.t1;Parent=MS.gene05608.t1

Gene Sequence	>MS.gene05608 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGGCATAGAAAGGTTCTTCGTGATAACATCCAGGGTATCACCAAACCTGCTATTCGTCGATTGGCAAGAAGAGGTGGTGTCAAGAGAATCAGTGGATTGATCTATGAGGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCAGTTACATACACTGAGCATGCAAGGAGGAAAACTGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene05608.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG