AlfalfaGEDB | MS.gene31086

M. sativa cultivar XinJiangDaYe / MS.gene31086

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31086.t1	XP_024632562.1	97.7	172	4	0	1	172	1	172	1.60E-90	342

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31086.t1	A2YEZ6	70.1	174	47	3	1	172	1	171	2.1e-64	246.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31086.t1	B7FGP6	97.7	172	4	0	1	172	1	172	1.1e-90	342.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050623	MS.gene31086	0.812541	4.02E-51	-1.69E-46
MS.gene051662	MS.gene31086	-0.813253	2.81E-51	-1.69E-46
MS.gene052048	MS.gene31086	0.978156	3.07E-145	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene24724	MS.gene31086	PPI
MS.gene050335	MS.gene31086	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31086.t1	MTR_2g098160	97.674	172	4	0	1	172	1	172	2.81e-124	347
MS.gene31086.t1	MTR_0249s0070	61.765	170	61	2	3	172	4	169	2.54e-76	225
MS.gene31086.t1	MTR_0249s0070	61.765	170	61	2	3	172	12	177	2.72e-76	226
MS.gene31086.t1	MTR_4g065570	77.622	143	25	2	31	172	1	137	1.36e-75	222
MS.gene31086.t1	MTR_7g092400	63.743	171	59	3	3	172	92	260	1.55e-75	227
MS.gene31086.t1	MTR_7g104320	46.061	165	79	3	12	172	10	168	1.59e-45	147
MS.gene31086.t1	MTR_4g053440	44.643	168	86	4	8	172	7	170	1.89e-42	139
MS.gene31086.t1	MTR_2g086190	44.304	158	71	5	15	172	10	150	4.60e-41	135
MS.gene31086.t1	MTR_1g060380	44.785	163	74	4	11	172	9	156	1.92e-37	126
MS.gene31086.t1	MTR_3g028010	37.821	156	77	2	17	172	28	163	7.51e-29	104
MS.gene31086.t1	MTR_8g036980	52.222	90	29	1	83	172	76	151	1.06e-27	101
MS.gene31086.t1	MTR_1g100773	33.333	156	84	4	15	170	3	138	7.22e-26	96.7
MS.gene31086.t1	MTR_7g114920	38.065	155	69	6	15	169	3	130	6.42e-25	94.0
MS.gene31086.t1	MTR_2g054650	33.333	156	70	5	15	170	4	125	6.32e-20	80.9
MS.gene31086.t1	MTR_3g025570	70.455	44	13	0	107	150	4	47	3.67e-19	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31086.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene31086.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene31086.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene31086.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene31086.t1	AT2G36320	47.879	165	71	4	11	172	9	161	4.94e-46	148
MS.gene31086.t1	AT4G12040	42.683	164	90	2	12	172	13	175	1.71e-45	147
MS.gene31086.t1	AT4G12040	42.683	164	90	2	12	172	13	175	1.71e-45	147
MS.gene31086.t1	AT2G27580	40.123	162	85	2	15	172	10	163	3.76e-42	139
MS.gene31086.t1	AT2G27580	40.123	162	85	2	15	172	10	163	3.76e-42	139
MS.gene31086.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene31086.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene31086.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene31086.t1	AT3G52800	43.210	162	92	0	11	172	9	170	7.58e-38	128
MS.gene31086.t1	AT3G52800	43.210	162	92	0	11	172	9	170	7.58e-38	128
MS.gene31086.t1	AT4G22820	38.509	161	93	3	15	172	19	176	6.48e-37	125
MS.gene31086.t1	AT4G22820	38.509	161	93	3	15	172	19	176	6.48e-37	125
MS.gene31086.t1	AT3G12630	39.333	150	71	2	23	172	31	160	7.01e-32	112
MS.gene31086.t1	AT4G14225	37.342	158	62	5	15	172	5	125	2.84e-27	99.8
MS.gene31086.t1	AT4G25380	32.903	155	70	4	18	172	10	130	1.58e-24	92.8

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCTGCAGAGATGGTTT+TGG	0.250126	2.4:+5871847	None:intergenic
GTTTCAGCATCATGTCTTTG+TGG	0.367335	2.4:+5871990	None:intergenic
CATCATGTCTTTGTGGCACT+TGG	0.399820	2.4:+5871997	None:intergenic
TTCCGGCTCAGAGGAGAGTT+TGG	0.400683	2.4:-5871816	MS.gene31086:CDS
CCAGCAGTGCGGTAATCAAA+TGG	0.411400	2.4:+5871665	None:intergenic
GCAGAACCGTTAGTTGCTTC+CGG	0.413723	2.4:-5871833	MS.gene31086:CDS
AATAAGCGGGTTGGTTTGAC+AGG	0.421004	2.4:-5871752	MS.gene31086:CDS
CAAAGACATGATGCTGAAAC+AGG	0.424095	2.4:-5871987	MS.gene31086:CDS
CACTTGCAGCATCTTCCATT+GGG	0.430249	2.4:-5871955	MS.gene31086:CDS
TTGCAGTTGCTGCACCGCTT+AGG	0.445309	2.4:+5871773	None:intergenic
TCCATTGGGAACATCATGAA+TGG	0.454077	2.4:-5871941	MS.gene31086:CDS
CCATTTGATTACCGCACTGC+TGG	0.477078	2.4:-5871665	MS.gene31086:CDS
ATGCACAACATAGGACCCTC+TGG	0.486214	2.4:+5872067	None:intergenic
ACACTTGCAGCATCTTCCAT+TGG	0.488087	2.4:-5871956	MS.gene31086:CDS
CTTCACATCCAGTCTGATCA+TGG	0.503432	2.4:+5872095	None:intergenic
CAGCAGTGCGGTAATCAAAT+GGG	0.506125	2.4:+5871666	None:intergenic
CACCGCTTAGGACCATCCTT+CGG	0.507315	2.4:+5871785	None:intergenic
TCCACGCTGGCAGTGATAGC+AGG	0.514469	2.4:+5871893	None:intergenic
CTCCAAACTCTCCTCTGAGC+CGG	0.515481	2.4:+5871814	None:intergenic
AACTAACGGTTCTGCAGAGA+TGG	0.516940	2.4:+5871841	None:intergenic
AGGTTCAATTACACTGCTGC+TGG	0.521288	2.4:+5871913	None:intergenic
GTGCAGCAACTGCAATAAGC+GGG	0.530850	2.4:-5871765	MS.gene31086:CDS
CCATTGGGAACATCATGAAT+GGG	0.543973	2.4:-5871940	MS.gene31086:CDS
AAAAGCAAACCCTGTTGTGA+AGG	0.563268	2.4:-5871627	MS.gene31086:CDS
GCACTTGGAACACATGTTCA+TGG	0.566316	2.4:+5872012	None:intergenic
TTGGAGAAGAAGCCGAAGGA+TGG	0.571839	2.4:-5871797	MS.gene31086:CDS
CCCATTCATGATGTTCCCAA+TGG	0.580215	2.4:+5871940	None:intergenic
TGCACAACATAGGACCCTCT+GGG	0.580560	2.4:+5872068	None:intergenic
AGCGATGTACAGCACAGTAA+AGG	0.582881	2.4:+5871708	None:intergenic
AGCAACTGCAATAAGCGGGT+TGG	0.585228	2.4:-5871761	MS.gene31086:CDS
ACAGGATTTAATTGTCGATG+TGG	0.589267	2.4:-5871734	MS.gene31086:CDS
CATGATGCTGAAACAGGAGC+AGG	0.593551	2.4:-5871981	MS.gene31086:CDS
CAGTTGTTGATGCACAACAT+AGG	0.601548	2.4:+5872058	None:intergenic
GGAGATCTCAGTCGATCAAG+TGG	0.604936	2.4:-5871873	MS.gene31086:CDS
AAGATGGACCATGATCAGAC+TGG	0.612119	2.4:-5872103	MS.gene31086:CDS
GGATGTGAAGCTGCCCCAGA+GGG	0.615247	2.4:-5872082	MS.gene31086:CDS
TCTGAGCCGGAAGCAACTAA+CGG	0.631746	2.4:+5871827	None:intergenic
GAGTTTGGAGAAGAAGCCGA+AGG	0.631849	2.4:-5871801	MS.gene31086:CDS
GGTGCAGCAACTGCAATAAG+CGG	0.632084	2.4:-5871766	MS.gene31086:CDS
AATGGAAGATGCTGCAAGTG+TGG	0.642024	2.4:+5871958	None:intergenic
GTTAGTTGCTTCCGGCTCAG+AGG	0.650978	2.4:-5871825	MS.gene31086:CDS
ATCGACTGAGATCTCCACGC+TGG	0.665451	2.4:+5871880	None:intergenic
TGGATGTGAAGCTGCCCCAG+AGG	0.681846	2.4:-5872083	MS.gene31086:CDS
ACCTGCTATCACTGCCAGCG+TGG	0.686751	2.4:-5871894	MS.gene31086:CDS
AGCCGAAGGATGGTCCTAAG+CGG	0.700565	2.4:-5871787	MS.gene31086:CDS
ATGTTGTGCATCAACAACTG+TGG	0.726175	2.4:-5872055	MS.gene31086:CDS
GCACAACATAGGACCCTCTG+GGG	0.736272	2.4:+5872069	None:intergenic
TTGCATCACGTCCAGCAGTG+CGG	0.742537	2.4:+5871654	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATCAACAACTGTGGATTTTT+CGG	-	chr2.4:5871658-5871677	MS.gene31086:CDS	30.0%
	ACAGGATTTAATTGTCGATG+TGG	-	chr2.4:5871970-5871989	MS.gene31086:CDS	35.0%
	AAAAGCAAACCCTGTTGTGA+AGG	-	chr2.4:5872077-5872096	MS.gene31086:CDS	40.0%
	ATGTTGTGCATCAACAACTG+TGG	-	chr2.4:5871649-5871668	MS.gene31086:CDS	40.0%
	CAAAGACATGATGCTGAAAC+AGG	-	chr2.4:5871717-5871736	MS.gene31086:CDS	40.0%
	CAGTTGTTGATGCACAACAT+AGG	+	chr2.4:5871649-5871668	None:intergenic	40.0%
	CCATTGGGAACATCATGAAT+GGG	-	chr2.4:5871764-5871783	MS.gene31086:CDS	40.0%
	GTTTCAGCATCATGTCTTTG+TGG	+	chr2.4:5871717-5871736	None:intergenic	40.0%
	TCCATTGGGAACATCATGAA+TGG	-	chr2.4:5871763-5871782	MS.gene31086:CDS	40.0%
	AACTAACGGTTCTGCAGAGA+TGG	+	chr2.4:5871866-5871885	None:intergenic	45.0%
	AATGGAAGATGCTGCAAGTG+TGG	+	chr2.4:5871749-5871768	None:intergenic	45.0%
	ACACTTGCAGCATCTTCCAT+TGG	-	chr2.4:5871748-5871767	MS.gene31086:CDS	45.0%
	AGCGATGTACAGCACAGTAA+AGG	+	chr2.4:5871999-5872018	None:intergenic	45.0%
	AGGTTCAATTACACTGCTGC+TGG	+	chr2.4:5871794-5871813	None:intergenic	45.0%
	CACTTGCAGCATCTTCCATT+GGG	-	chr2.4:5871749-5871768	MS.gene31086:CDS	45.0%
	CAGCAGTGCGGTAATCAAAT+GGG	+	chr2.4:5872041-5872060	None:intergenic	45.0%
	CATCATGTCTTTGTGGCACT+TGG	+	chr2.4:5871710-5871729	None:intergenic	45.0%
	CCCATTCATGATGTTCCCAA+TGG	+	chr2.4:5871767-5871786	None:intergenic	45.0%
	CTTCACATCCAGTCTGATCA+TGG	+	chr2.4:5871612-5871631	None:intergenic	45.0%
	GCACTTGGAACACATGTTCA+TGG	+	chr2.4:5871695-5871714	None:intergenic	45.0%
!	AGCTTTTCAGCCTTCACAAC+AGG	+	chr2.4:5872090-5872109	None:intergenic	45.0%
!	GCTTTTCAGCCTTCACAACA+GGG	+	chr2.4:5872089-5872108	None:intergenic	45.0%
!	GGTTCTGCAGAGATGGTTTT+GGG	+	chr2.4:5871859-5871878	None:intergenic	45.0%
!!	AATAAGCGGGTTGGTTTGAC+AGG	-	chr2.4:5871952-5871971	MS.gene31086:CDS	45.0%
	AGCAACTGCAATAAGCGGGT+TGG	-	chr2.4:5871943-5871962	MS.gene31086:CDS	50.0%
	ATGCACAACATAGGACCCTC+TGG	+	chr2.4:5871640-5871659	None:intergenic	50.0%
	CATGATGCTGAAACAGGAGC+AGG	-	chr2.4:5871723-5871742	MS.gene31086:CDS	50.0%
	CCAGCAGTGCGGTAATCAAA+TGG	+	chr2.4:5872042-5872061	None:intergenic	50.0%
	CCATTTGATTACCGCACTGC+TGG	-	chr2.4:5872039-5872058	MS.gene31086:CDS	50.0%
	GAGTTTGGAGAAGAAGCCGA+AGG	-	chr2.4:5871903-5871922	MS.gene31086:CDS	50.0%
	GCAGAACCGTTAGTTGCTTC+CGG	-	chr2.4:5871871-5871890	MS.gene31086:CDS	50.0%
	GGAGATCTCAGTCGATCAAG+TGG	-	chr2.4:5871831-5871850	MS.gene31086:CDS	50.0%
	GGTGCAGCAACTGCAATAAG+CGG	-	chr2.4:5871938-5871957	MS.gene31086:CDS	50.0%
	GTGCAGCAACTGCAATAAGC+GGG	-	chr2.4:5871939-5871958	MS.gene31086:CDS	50.0%
	TCTGAGCCGGAAGCAACTAA+CGG	+	chr2.4:5871880-5871899	None:intergenic	50.0%
	TGCACAACATAGGACCCTCT+GGG	+	chr2.4:5871639-5871658	None:intergenic	50.0%
	TTGGAGAAGAAGCCGAAGGA+TGG	-	chr2.4:5871907-5871926	MS.gene31086:CDS	50.0%
!	CGGTTCTGCAGAGATGGTTT+TGG	+	chr2.4:5871860-5871879	None:intergenic	50.0%
	AGCCGAAGGATGGTCCTAAG+CGG	-	chr2.4:5871917-5871936	MS.gene31086:CDS	55.0%
	ATCGACTGAGATCTCCACGC+TGG	+	chr2.4:5871827-5871846	None:intergenic	55.0%
	CACCGCTTAGGACCATCCTT+CGG	+	chr2.4:5871922-5871941	None:intergenic	55.0%
	CTCCAAACTCTCCTCTGAGC+CGG	+	chr2.4:5871893-5871912	None:intergenic	55.0%
	GCACAACATAGGACCCTCTG+GGG	+	chr2.4:5871638-5871657	None:intergenic	55.0%
	GTTAGTTGCTTCCGGCTCAG+AGG	-	chr2.4:5871879-5871898	MS.gene31086:CDS	55.0%
	TTCCGGCTCAGAGGAGAGTT+TGG	-	chr2.4:5871888-5871907	MS.gene31086:CDS	55.0%
	TTGCAGTTGCTGCACCGCTT+AGG	+	chr2.4:5871934-5871953	None:intergenic	55.0%
	TTGCATCACGTCCAGCAGTG+CGG	+	chr2.4:5872053-5872072	None:intergenic	55.0%
	ACCTGCTATCACTGCCAGCG+TGG	-	chr2.4:5871810-5871829	MS.gene31086:CDS	60.0%
	GGATGTGAAGCTGCCCCAGA+GGG	-	chr2.4:5871622-5871641	MS.gene31086:CDS	60.0%
	TCCACGCTGGCAGTGATAGC+AGG	+	chr2.4:5871814-5871833	None:intergenic	60.0%
	TGGATGTGAAGCTGCCCCAG+AGG	-	chr2.4:5871621-5871640	MS.gene31086:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	5871604	5872122	5871604	ID=MS.gene31086
chr2.4	mRNA	5871604	5872122	5871604	ID=MS.gene31086.t1;Parent=MS.gene31086
chr2.4	exon	5871604	5872122	5871604	ID=MS.gene31086.t1.exon1;Parent=MS.gene31086.t1
chr2.4	CDS	5871604	5872122	5871604	ID=cds.MS.gene31086.t1;Parent=MS.gene31086.t1

Gene Sequence

>MS.gene31086

ATGGACCATGATCAGACTGGATGTGAAGCTGCCCCAGAGGGTCCTATGTTGTGCATCAACAACTGTGGATTTTTCGGAAGCGCAGCTACCATGAACATGTGTTCCAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCACACTTGCAGCATCTTCCATTGGGAACATCATGAATGGGTCATCCAGCAGCAGTGTAATTGAACCTGCTATCACTGCCAGCGTGGAGATCTCAGTCGATCAAGTGGAGCCCAAAACCATCTCTGCAGAACCGTTAGTTGCTTCCGGCTCAGAGGAGAGTTTGGAGAAGAAGCCGAAGGATGGTCCTAAGCGGTGCAGCAACTGCAATAAGCGGGTTGGTTTGACAGGATTTAATTGTCGATGTGGTAACCTTTACTGTGCTGTACATCGCTACTCAGACAAGCATGACTGCCCATTTGATTACCGCACTGCTGGACGTGATGCAATAGCAAAAGCAAACCCTGTTGTGAAGGCTGAAAAGCTTGACAAGATCTAG

Protein sequence

>MS.gene31086.t1

MDHDQTGCEAAPEGPMLCINNCGFFGSAATMNMCSKCHKDMMLKQEQATLAASSIGNIMNGSSSSSVIEPAITASVEISVDQVEPKTISAEPLVASGSEESLEKKPKDGPKRCSNCNKRVGLTGFNCRCGNLYCAVHRYSDKHDCPFDYRTAGRDAIAKANPVVKAEKLDKI