AlfalfaGEDB | MS.gene052048

M. sativa cultivar XinJiangDaYe / MS.gene052048

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052048.t1	XP_024632562.1	97.7	172	4	0	1	172	1	172	1.60E-90	342

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052048.t1	A2YEZ6	70.1	174	47	3	1	172	1	171	2.1e-64	246.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052048.t1	B7FGP6	97.7	172	4	0	1	172	1	172	1.1e-90	342.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050623	MS.gene052048	0.844282	8.33E-59	-1.69E-46
MS.gene051662	MS.gene052048	-0.828098	1.09E-54	-1.69E-46
MS.gene052048	MS.gene054942	-0.813823	2.11E-51	-1.69E-46
MS.gene052048	MS.gene055591	0.82087	5.46E-53	-1.69E-46
MS.gene052048	MS.gene058661	0.806343	8.61E-50	-1.69E-46
MS.gene052048	MS.gene06420	-0.813355	2.67E-51	-1.69E-46
MS.gene052048	MS.gene06594	-0.834879	2.32E-56	-1.69E-46
MS.gene052048	MS.gene06806	-0.83397	3.93E-56	-1.69E-46
MS.gene052048	MS.gene068549	-0.808634	2.81E-50	-1.69E-46
MS.gene052048	MS.gene068972	-0.822536	2.25E-53	-1.69E-46
MS.gene052048	MS.gene070036	-0.800218	1.60E-48	-1.69E-46
MS.gene052048	MS.gene07089	-0.80211	6.55E-49	-1.69E-46
MS.gene052048	MS.gene071438	0.806604	7.59E-50	-1.69E-46
MS.gene052048	MS.gene071711	0.808573	2.90E-50	-1.69E-46
MS.gene052048	MS.gene07368	-0.822629	2.14E-53	-1.69E-46
MS.gene052048	MS.gene074465	-0.808433	3.10E-50	-1.69E-46
MS.gene052048	MS.gene20912	-0.812874	3.40E-51	-1.69E-46
MS.gene052048	MS.gene22820	0.833883	4.13E-56	-1.69E-46
MS.gene052048	MS.gene22983	-0.833923	4.03E-56	-1.69E-46
MS.gene052048	MS.gene28922	0.800275	1.56E-48	-1.69E-46
MS.gene052048	MS.gene30855	-0.807974	3.89E-50	-1.69E-46
MS.gene052048	MS.gene31040	0.813283	2.77E-51	-1.69E-46
MS.gene052048	MS.gene31086	0.978156	3.07E-145	-1.69E-46
MS.gene052048	MS.gene36120	-0.826678	2.38E-54	-1.69E-46
MS.gene052048	MS.gene36483	0.807709	4.43E-50	-1.69E-46
MS.gene052048	MS.gene42724	-0.807269	5.49E-50	-1.69E-46
MS.gene052048	MS.gene45557	0.99031	4.97E-182	-1.69E-46
MS.gene052048	MS.gene53383	-0.805881	1.08E-49	-1.69E-46
MS.gene052048	MS.gene57162	0.80205	6.74E-49	-1.69E-46
MS.gene052048	MS.gene57577	0.802871	4.57E-49	-1.69E-46
MS.gene052048	MS.gene59876	-0.800087	1.70E-48	-1.69E-46
MS.gene052048	MS.gene60781	0.80003	1.75E-48	-1.69E-46
MS.gene052048	MS.gene70989	0.847169	1.37E-59	-1.69E-46
MS.gene052048	MS.gene72851	0.849033	4.19E-60	-1.69E-46
MS.gene052048	MS.gene77584	0.80424	2.38E-49	-1.69E-46
MS.gene052048	MS.gene78247	-0.808455	3.07E-50	-1.69E-46
MS.gene052048	MS.gene86380	-0.82352	1.33E-53	-1.69E-46
MS.gene052048	MS.gene88831	0.81674	4.73E-52	-1.69E-46
MS.gene052048	MS.gene92548	-0.809061	2.28E-50	-1.69E-46
MS.gene052048	MS.gene93736	0.81228	4.59E-51	-1.69E-46
MS.gene052048	MS.gene94818	0.814521	1.48E-51	-1.69E-46
MS.gene052048	MS.gene96220	-0.817655	2.95E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene050335	MS.gene052048	PPI
MS.gene052048	MS.gene24724	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052048.t1	MTR_2g098160	97.674	172	4	0	1	172	1	172	2.81e-124	347
MS.gene052048.t1	MTR_0249s0070	61.765	170	61	2	3	172	4	169	2.54e-76	225
MS.gene052048.t1	MTR_0249s0070	61.765	170	61	2	3	172	12	177	2.72e-76	226
MS.gene052048.t1	MTR_4g065570	77.622	143	25	2	31	172	1	137	1.36e-75	222
MS.gene052048.t1	MTR_7g092400	63.743	171	59	3	3	172	92	260	1.55e-75	227
MS.gene052048.t1	MTR_7g104320	46.061	165	79	3	12	172	10	168	1.59e-45	147
MS.gene052048.t1	MTR_4g053440	44.643	168	86	4	8	172	7	170	1.89e-42	139
MS.gene052048.t1	MTR_2g086190	44.304	158	71	5	15	172	10	150	4.60e-41	135
MS.gene052048.t1	MTR_1g060380	44.785	163	74	4	11	172	9	156	1.92e-37	126
MS.gene052048.t1	MTR_3g028010	37.821	156	77	2	17	172	28	163	7.51e-29	104
MS.gene052048.t1	MTR_8g036980	52.222	90	29	1	83	172	76	151	1.06e-27	101
MS.gene052048.t1	MTR_1g100773	33.333	156	84	4	15	170	3	138	7.22e-26	96.7
MS.gene052048.t1	MTR_7g114920	38.065	155	69	6	15	169	3	130	6.42e-25	94.0
MS.gene052048.t1	MTR_2g054650	33.333	156	70	5	15	170	4	125	6.32e-20	80.9
MS.gene052048.t1	MTR_3g025570	70.455	44	13	0	107	150	4	47	3.67e-19	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052048.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene052048.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene052048.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene052048.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene052048.t1	AT2G36320	47.879	165	71	4	11	172	9	161	4.94e-46	148
MS.gene052048.t1	AT4G12040	42.683	164	90	2	12	172	13	175	1.71e-45	147
MS.gene052048.t1	AT4G12040	42.683	164	90	2	12	172	13	175	1.71e-45	147
MS.gene052048.t1	AT2G27580	40.123	162	85	2	15	172	10	163	3.76e-42	139
MS.gene052048.t1	AT2G27580	40.123	162	85	2	15	172	10	163	3.76e-42	139
MS.gene052048.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene052048.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene052048.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene052048.t1	AT3G52800	43.210	162	92	0	11	172	9	170	7.58e-38	128
MS.gene052048.t1	AT3G52800	43.210	162	92	0	11	172	9	170	7.58e-38	128
MS.gene052048.t1	AT4G22820	38.509	161	93	3	15	172	19	176	6.48e-37	125
MS.gene052048.t1	AT4G22820	38.509	161	93	3	15	172	19	176	6.48e-37	125
MS.gene052048.t1	AT3G12630	39.333	150	71	2	23	172	31	160	7.01e-32	112
MS.gene052048.t1	AT4G14225	37.342	158	62	5	15	172	5	125	2.84e-27	99.8
MS.gene052048.t1	AT4G25380	32.903	155	70	4	18	172	10	130	1.58e-24	92.8

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCTGCAGAGATGGTTT+TGG	0.250126	2.2:+4513083	None:intergenic
GTTTCAGCATCATGTCTTTG+TGG	0.367335	2.2:+4513226	None:intergenic
CATCATGTCTTTGTGGCACT+TGG	0.399820	2.2:+4513233	None:intergenic
TTCCGGCTCAGAGGAGAGTT+TGG	0.400683	2.2:-4513052	MS.gene052048:CDS
CCAGCAGTGCGGTAATCAAA+TGG	0.411400	2.2:+4512901	None:intergenic
GCAGAACCGTTAGTTGCTTC+CGG	0.413723	2.2:-4513069	MS.gene052048:CDS
AATAAGCGGGTTGGTTTGAC+AGG	0.421004	2.2:-4512988	MS.gene052048:CDS
CAAAGACATGATGCTGAAAC+AGG	0.424095	2.2:-4513223	MS.gene052048:CDS
CACTTGCAGCATCTTCCATT+GGG	0.430249	2.2:-4513191	MS.gene052048:CDS
TTGCAGTTGCTGCACCGCTT+AGG	0.445309	2.2:+4513009	None:intergenic
TCCATTGGGAACATCATGAA+TGG	0.454077	2.2:-4513177	MS.gene052048:CDS
CCATTTGATTACCGCACTGC+TGG	0.477078	2.2:-4512901	MS.gene052048:CDS
ATGCACAACATAGGACCCTC+TGG	0.486214	2.2:+4513303	None:intergenic
ACACTTGCAGCATCTTCCAT+TGG	0.488087	2.2:-4513192	MS.gene052048:CDS
CTTCACATCCAGTCTGATCA+TGG	0.503432	2.2:+4513331	None:intergenic
CAGCAGTGCGGTAATCAAAT+GGG	0.506125	2.2:+4512902	None:intergenic
CACCGCTTAGGACCATCCTT+CGG	0.507315	2.2:+4513021	None:intergenic
TCCACGCTGGCAGTGATAGC+AGG	0.514469	2.2:+4513129	None:intergenic
CTCCAAACTCTCCTCTGAGC+CGG	0.515481	2.2:+4513050	None:intergenic
AACTAACGGTTCTGCAGAGA+TGG	0.516940	2.2:+4513077	None:intergenic
AGGTTCAATTACACTGCTGC+TGG	0.521288	2.2:+4513149	None:intergenic
GTGCAGCAACTGCAATAAGC+GGG	0.530850	2.2:-4513001	MS.gene052048:CDS
CCATTGGGAACATCATGAAT+GGG	0.543973	2.2:-4513176	MS.gene052048:CDS
AAAAGCAAACCCTGTTGTGA+AGG	0.563268	2.2:-4512863	MS.gene052048:CDS
GCACTTGGAACACATGTTCA+TGG	0.566316	2.2:+4513248	None:intergenic
TTGGAGAAGAAGCCGAAGGA+TGG	0.571839	2.2:-4513033	MS.gene052048:CDS
CCCATTCATGATGTTCCCAA+TGG	0.580215	2.2:+4513176	None:intergenic
TGCACAACATAGGACCCTCT+GGG	0.580560	2.2:+4513304	None:intergenic
AGCGATGTACAGCACAGTAA+AGG	0.582881	2.2:+4512944	None:intergenic
AGCAACTGCAATAAGCGGGT+TGG	0.585228	2.2:-4512997	MS.gene052048:CDS
ACAGGATTTAATTGTCGATG+TGG	0.589267	2.2:-4512970	MS.gene052048:CDS
CATGATGCTGAAACAGGAGC+AGG	0.593551	2.2:-4513217	MS.gene052048:CDS
CAGTTGTTGATGCACAACAT+AGG	0.601548	2.2:+4513294	None:intergenic
GGAGATCTCAGTCGATCAAG+TGG	0.604936	2.2:-4513109	MS.gene052048:CDS
AAGATGGACCATGATCAGAC+TGG	0.612119	2.2:-4513339	MS.gene052048:CDS
GGATGTGAAGCTGCCCCAGA+GGG	0.615247	2.2:-4513318	MS.gene052048:CDS
TCTGAGCCGGAAGCAACTAA+CGG	0.631746	2.2:+4513063	None:intergenic
GAGTTTGGAGAAGAAGCCGA+AGG	0.631849	2.2:-4513037	MS.gene052048:CDS
GGTGCAGCAACTGCAATAAG+CGG	0.632084	2.2:-4513002	MS.gene052048:CDS
AATGGAAGATGCTGCAAGTG+TGG	0.642024	2.2:+4513194	None:intergenic
GTTAGTTGCTTCCGGCTCAG+AGG	0.650978	2.2:-4513061	MS.gene052048:CDS
ATCGACTGAGATCTCCACGC+TGG	0.665451	2.2:+4513116	None:intergenic
TGGATGTGAAGCTGCCCCAG+AGG	0.681846	2.2:-4513319	MS.gene052048:CDS
ACCTGCTATCACTGCCAGCG+TGG	0.686751	2.2:-4513130	MS.gene052048:CDS
AGCCGAAGGATGGTCCTAAG+CGG	0.700565	2.2:-4513023	MS.gene052048:CDS
ATGTTGTGCATCAACAACTG+TGG	0.726175	2.2:-4513291	MS.gene052048:CDS
GCACAACATAGGACCCTCTG+GGG	0.736272	2.2:+4513305	None:intergenic
TTGCATCACGTCCAGCAGTG+CGG	0.742537	2.2:+4512890	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATCAACAACTGTGGATTTTT+CGG	-	chr2.2:4512894-4512913	MS.gene052048:CDS	30.0%
	ACAGGATTTAATTGTCGATG+TGG	-	chr2.2:4513206-4513225	MS.gene052048:CDS	35.0%
	AAAAGCAAACCCTGTTGTGA+AGG	-	chr2.2:4513313-4513332	MS.gene052048:CDS	40.0%
	ATGTTGTGCATCAACAACTG+TGG	-	chr2.2:4512885-4512904	MS.gene052048:CDS	40.0%
	CAAAGACATGATGCTGAAAC+AGG	-	chr2.2:4512953-4512972	MS.gene052048:CDS	40.0%
	CAGTTGTTGATGCACAACAT+AGG	+	chr2.2:4512885-4512904	None:intergenic	40.0%
	CCATTGGGAACATCATGAAT+GGG	-	chr2.2:4513000-4513019	MS.gene052048:CDS	40.0%
	GTTTCAGCATCATGTCTTTG+TGG	+	chr2.2:4512953-4512972	None:intergenic	40.0%
	TCCATTGGGAACATCATGAA+TGG	-	chr2.2:4512999-4513018	MS.gene052048:CDS	40.0%
	AACTAACGGTTCTGCAGAGA+TGG	+	chr2.2:4513102-4513121	None:intergenic	45.0%
	AATGGAAGATGCTGCAAGTG+TGG	+	chr2.2:4512985-4513004	None:intergenic	45.0%
	ACACTTGCAGCATCTTCCAT+TGG	-	chr2.2:4512984-4513003	MS.gene052048:CDS	45.0%
	AGCGATGTACAGCACAGTAA+AGG	+	chr2.2:4513235-4513254	None:intergenic	45.0%
	AGGTTCAATTACACTGCTGC+TGG	+	chr2.2:4513030-4513049	None:intergenic	45.0%
	CACTTGCAGCATCTTCCATT+GGG	-	chr2.2:4512985-4513004	MS.gene052048:CDS	45.0%
	CAGCAGTGCGGTAATCAAAT+GGG	+	chr2.2:4513277-4513296	None:intergenic	45.0%
	CATCATGTCTTTGTGGCACT+TGG	+	chr2.2:4512946-4512965	None:intergenic	45.0%
	CCCATTCATGATGTTCCCAA+TGG	+	chr2.2:4513003-4513022	None:intergenic	45.0%
	CTTCACATCCAGTCTGATCA+TGG	+	chr2.2:4512848-4512867	None:intergenic	45.0%
	GCACTTGGAACACATGTTCA+TGG	+	chr2.2:4512931-4512950	None:intergenic	45.0%
!	AGCTTTTCAGCCTTCACAAC+AGG	+	chr2.2:4513326-4513345	None:intergenic	45.0%
!	GCTTTTCAGCCTTCACAACA+GGG	+	chr2.2:4513325-4513344	None:intergenic	45.0%
!	GGTTCTGCAGAGATGGTTTT+GGG	+	chr2.2:4513095-4513114	None:intergenic	45.0%
!!	AATAAGCGGGTTGGTTTGAC+AGG	-	chr2.2:4513188-4513207	MS.gene052048:CDS	45.0%
	AGCAACTGCAATAAGCGGGT+TGG	-	chr2.2:4513179-4513198	MS.gene052048:CDS	50.0%
	ATGCACAACATAGGACCCTC+TGG	+	chr2.2:4512876-4512895	None:intergenic	50.0%
	CATGATGCTGAAACAGGAGC+AGG	-	chr2.2:4512959-4512978	MS.gene052048:CDS	50.0%
	CCAGCAGTGCGGTAATCAAA+TGG	+	chr2.2:4513278-4513297	None:intergenic	50.0%
	CCATTTGATTACCGCACTGC+TGG	-	chr2.2:4513275-4513294	MS.gene052048:CDS	50.0%
	GAGTTTGGAGAAGAAGCCGA+AGG	-	chr2.2:4513139-4513158	MS.gene052048:CDS	50.0%
	GCAGAACCGTTAGTTGCTTC+CGG	-	chr2.2:4513107-4513126	MS.gene052048:CDS	50.0%
	GGAGATCTCAGTCGATCAAG+TGG	-	chr2.2:4513067-4513086	MS.gene052048:CDS	50.0%
	GGTGCAGCAACTGCAATAAG+CGG	-	chr2.2:4513174-4513193	MS.gene052048:CDS	50.0%
	GTGCAGCAACTGCAATAAGC+GGG	-	chr2.2:4513175-4513194	MS.gene052048:CDS	50.0%
	TCTGAGCCGGAAGCAACTAA+CGG	+	chr2.2:4513116-4513135	None:intergenic	50.0%
	TGCACAACATAGGACCCTCT+GGG	+	chr2.2:4512875-4512894	None:intergenic	50.0%
	TTGGAGAAGAAGCCGAAGGA+TGG	-	chr2.2:4513143-4513162	MS.gene052048:CDS	50.0%
!	CGGTTCTGCAGAGATGGTTT+TGG	+	chr2.2:4513096-4513115	None:intergenic	50.0%
	AGCCGAAGGATGGTCCTAAG+CGG	-	chr2.2:4513153-4513172	MS.gene052048:CDS	55.0%
	ATCGACTGAGATCTCCACGC+TGG	+	chr2.2:4513063-4513082	None:intergenic	55.0%
	CACCGCTTAGGACCATCCTT+CGG	+	chr2.2:4513158-4513177	None:intergenic	55.0%
	CTCCAAACTCTCCTCTGAGC+CGG	+	chr2.2:4513129-4513148	None:intergenic	55.0%
	GCACAACATAGGACCCTCTG+GGG	+	chr2.2:4512874-4512893	None:intergenic	55.0%
	GTTAGTTGCTTCCGGCTCAG+AGG	-	chr2.2:4513115-4513134	MS.gene052048:CDS	55.0%
	TTCCGGCTCAGAGGAGAGTT+TGG	-	chr2.2:4513124-4513143	MS.gene052048:CDS	55.0%
	TTGCAGTTGCTGCACCGCTT+AGG	+	chr2.2:4513170-4513189	None:intergenic	55.0%
	TTGCATCACGTCCAGCAGTG+CGG	+	chr2.2:4513289-4513308	None:intergenic	55.0%
	ACCTGCTATCACTGCCAGCG+TGG	-	chr2.2:4513046-4513065	MS.gene052048:CDS	60.0%
	GGATGTGAAGCTGCCCCAGA+GGG	-	chr2.2:4512858-4512877	MS.gene052048:CDS	60.0%
	TCCACGCTGGCAGTGATAGC+AGG	+	chr2.2:4513050-4513069	None:intergenic	60.0%
	TGGATGTGAAGCTGCCCCAG+AGG	-	chr2.2:4512857-4512876	MS.gene052048:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	4512840	4513358	4512840	ID=MS.gene052048
chr2.2	mRNA	4512840	4513358	4512840	ID=MS.gene052048.t1;Parent=MS.gene052048
chr2.2	exon	4512840	4513358	4512840	ID=MS.gene052048.t1.exon1;Parent=MS.gene052048.t1
chr2.2	CDS	4512840	4513358	4512840	ID=cds.MS.gene052048.t1;Parent=MS.gene052048.t1

Gene Sequence

>MS.gene052048

ATGGACCATGATCAGACTGGATGTGAAGCTGCCCCAGAGGGTCCTATGTTGTGCATCAACAACTGTGGATTTTTCGGAAGCGCAGCTACCATGAACATGTGTTCCAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCACACTTGCAGCATCTTCCATTGGGAACATCATGAATGGGTCATCCAGCAGCAGTGTAATTGAACCTGCTATCACTGCCAGCGTGGAGATCTCAGTCGATCAAGTGGAGCCCAAAACCATCTCTGCAGAACCGTTAGTTGCTTCCGGCTCAGAGGAGAGTTTGGAGAAGAAGCCGAAGGATGGTCCTAAGCGGTGCAGCAACTGCAATAAGCGGGTTGGTTTGACAGGATTTAATTGTCGATGTGGTAACCTTTACTGTGCTGTACATCGCTACTCAGACAAGCATGACTGCCCATTTGATTACCGCACTGCTGGACGTGATGCAATAGCAAAAGCAAACCCTGTTGTGAAGGCTGAAAAGCTTGACAAGATCTAG

Protein sequence

>MS.gene052048.t1

MDHDQTGCEAAPEGPMLCINNCGFFGSAATMNMCSKCHKDMMLKQEQATLAASSIGNIMNGSSSSSVIEPAITASVEISVDQVEPKTISAEPLVASGSEESLEKKPKDGPKRCSNCNKRVGLTGFNCRCGNLYCAVHRYSDKHDCPFDYRTAGRDAIAKANPVVKAEKLDKI