AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31289

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31289.t1 MTR_4g063670 89.688 320 26 1 1 313 1 320 0.0 568
MS.gene31289.t1 MTR_0867s0010 71.473 319 84 1 1 312 1 319 6.47e-152 429
MS.gene31289.t1 MTR_4g083510 68.224 321 94 2 1 313 1 321 3.56e-150 424
MS.gene31289.t1 MTR_1g051185 77.320 291 34 2 1 284 1 266 1.56e-149 421
MS.gene31289.t1 MTR_3g069460 84.483 174 27 0 1 174 1 174 3.79e-108 312
MS.gene31289.t1 MTR_8g015750 63.861 202 50 3 118 312 1 186 8.09e-72 220
MS.gene31289.t1 MTR_7g088160 40.432 324 150 9 21 312 76 388 3.55e-62 202
MS.gene31289.t1 MTR_7g107340 38.019 313 156 10 6 312 54 334 5.17e-47 161
MS.gene31289.t1 MTR_8g015740 68.293 82 26 0 1 82 49 130 7.79e-34 120
MS.gene31289.t1 MTR_7g114170 33.123 317 134 12 6 311 39 288 1.49e-26 106
MS.gene31289.t1 MTR_6g081150 31.416 226 90 8 19 244 16 176 3.83e-20 86.3
MS.gene31289.t1 MTR_2g023560 68.966 58 18 0 50 107 61 118 1.18e-19 83.2
MS.gene31289.t1 MTR_4g013220 26.432 227 154 6 22 237 55 279 1.21e-16 79.7
MS.gene31289.t1 MTR_6g453050 27.322 183 117 6 22 191 55 234 4.52e-16 77.8
MS.gene31289.t1 MTR_3g067785 25.991 227 155 6 22 237 55 279 8.15e-16 77.0
MS.gene31289.t1 MTR_4g026120 25.991 227 155 6 22 237 55 279 8.97e-16 77.0
MS.gene31289.t1 MTR_4g036435 25.991 227 155 6 22 237 55 279 8.97e-16 77.0
MS.gene31289.t1 MTR_5g066280 25.991 227 155 6 22 237 55 279 9.32e-16 77.0
MS.gene31289.t1 MTR_1g106885 27.027 222 138 6 22 234 116 322 3.88e-15 75.9
MS.gene31289.t1 MTR_5g077245 26.761 213 143 6 36 237 122 332 4.22e-15 75.5
MS.gene31289.t1 MTR_2g022120 26.341 205 127 6 22 217 119 308 4.24e-15 75.5
MS.gene31289.t1 MTR_7g062380 25.352 213 146 6 36 237 4 214 5.84e-15 73.9
MS.gene31289.t1 MTR_2g072860 28.448 116 81 2 59 174 72 185 6.78e-15 74.3
MS.gene31289.t1 MTR_7g461350 29.310 116 80 2 59 174 140 253 7.02e-15 74.7
MS.gene31289.t1 MTR_6g016670 29.310 116 80 2 59 174 140 253 7.02e-15 74.7
MS.gene31289.t1 MTR_3g464310 29.310 116 80 2 59 174 140 253 7.02e-15 74.7
MS.gene31289.t1 MTR_7g083520 28.378 148 99 4 27 170 37 181 8.13e-15 73.9
MS.gene31289.t1 MTR_3g464170 28.448 116 81 2 59 174 140 253 1.37e-14 73.9
MS.gene31289.t1 MTR_1g100375 28.448 116 81 2 59 174 140 253 1.37e-14 73.9
MS.gene31289.t1 MTR_6g051405 28.448 116 81 2 59 174 140 253 1.48e-14 73.9
MS.gene31289.t1 MTR_3g450670 28.448 116 81 2 59 174 136 249 1.59e-14 73.9
MS.gene31289.t1 MTR_1g471070 28.448 116 81 2 59 174 136 249 1.59e-14 73.9
MS.gene31289.t1 MTR_6g092533 27.673 159 107 4 22 174 119 275 2.66e-14 73.2
MS.gene31289.t1 MTR_5g044920 75.000 40 10 0 43 82 17 56 2.74e-13 65.1
MS.gene31289.t1 MTR_1g086750 31.858 113 75 2 59 171 33 143 1.17e-12 66.6
MS.gene31289.t1 MTR_4g046090 27.273 132 91 3 68 196 67 196 6.54e-12 65.1
MS.gene31289.t1 MTR_6g069240 25.658 152 101 4 71 222 19 158 2.72e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31289.t1 AT3G47680 37.619 210 119 6 30 232 23 227 3.53e-29 113
MS.gene31289.t1 AT5G41220 40.884 181 90 7 56 222 219 396 6.42e-28 113
MS.gene31289.t1 AT5G41220 40.884 181 90 7 56 222 227 404 7.83e-28 113
MS.gene31289.t1 AT5G41220 40.884 181 90 7 56 222 277 454 8.07e-28 113
MS.gene31289.t1 AT5G41240 37.297 185 102 6 51 222 272 455 2.42e-27 112
MS.gene31289.t1 AT1G66235 25.346 217 139 5 56 256 11 220 2.15e-13 69.7

Find 60 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCGAGAGCGACATTATTT+TGG 0.218289 2.4:-8033061 MS.gene31289:CDS
ATAAACCTTGATGAAGAAAT+TGG 0.250877 2.4:-8033372 MS.gene31289:CDS
AGGAATTTCATGGCACTTAA+AGG 0.293316 2.4:-8033168 MS.gene31289:CDS
TAATGAACCTGCACTCCATT+TGG 0.310744 2.4:+8032960 None:intergenic
CCTTCTGTTAATAAGTTTGT+TGG 0.320088 2.4:-8033123 MS.gene31289:CDS
GGAAAAGGGTTGAAGATGAT+AGG 0.352254 2.4:-8032672 MS.gene31289:CDS
AATGAACCTGCACTCCATTT+GGG 0.366609 2.4:+8032961 None:intergenic
TGATTTACAAATTCTCTTGA+AGG 0.367301 2.4:-8032632 MS.gene31289:CDS
GCCGGATTATATTCGCTTGT+TGG 0.397343 2.4:+8032868 None:intergenic
AAGCCGAGATAGAGAGGAAA+AGG 0.406812 2.4:-8032687 MS.gene31289:CDS
CGGATCCAATTTCTTCATCA+AGG 0.413303 2.4:+8033367 None:intergenic
ATTGTTGGGATTGATCAAAA+AGG 0.426909 2.4:-8033255 MS.gene31289:CDS
TCGGTCAAAAGGCGGCTAAA+AGG 0.428904 2.4:-8032834 MS.gene31289:CDS
TCATAATCGCGTAGTTGTCT+TGG 0.431755 2.4:+8032592 None:intergenic
TGCATAATCACGTGCAAACT+CGG 0.432397 2.4:+8032726 None:intergenic
ATGAGAAGTTCATCCTCGTT+TGG 0.433365 2.4:+8033309 None:intergenic
TTTCAAAGGACTCGATTGTT+GGG 0.442182 2.4:-8033269 MS.gene31289:CDS
AGAACAAAGAATTCAACTTC+CGG 0.447947 2.4:-8032922 MS.gene31289:CDS
ATTTCAAAGGACTCGATTGT+TGG 0.449581 2.4:-8033270 MS.gene31289:CDS
TTGTCCAGAGAGGAATTTCA+TGG 0.464666 2.4:-8033178 MS.gene31289:CDS
ATCTTGTACTCAAGGTGGCT+TGG 0.464998 2.4:-8033400 MS.gene31289:CDS
GAGGTTCAAATTGATAATAA+TGG 0.466188 2.4:-8033459 MS.gene31289:CDS
TAATGAAGGATGAGCCCAAA+TGG 0.470192 2.4:-8032975 MS.gene31289:CDS
TATTCGCTTGTTGGTGTTGG+TGG 0.471434 2.4:+8032877 None:intergenic
ATGAACTTCTCATTCAATCA+TGG 0.473430 2.4:-8033299 MS.gene31289:CDS
GCGTAGAAAATTCACATTTG+AGG 0.485091 2.4:-8033010 MS.gene31289:CDS
GAAAAGGGTTGAAGATGATA+GGG 0.487637 2.4:-8032671 MS.gene31289:CDS
TTATATTCGCTTGTTGGTGT+TGG 0.496844 2.4:+8032874 None:intergenic
AATCCGGCGAAGCGTCCAAT+CGG 0.499982 2.4:-8032853 MS.gene31289:CDS
TGACCGATTGGACGCTTCGC+CGG 0.505561 2.4:+8032850 None:intergenic
AGTGCCATGAAATTCCTCTC+TGG 0.513566 2.4:+8033174 None:intergenic
GAAGCGTCCAATCGGTCAAA+AGG 0.517129 2.4:-8032845 MS.gene31289:CDS
ATCATGGTTCAACATTTCAA+AGG 0.518437 2.4:-8033283 MS.gene31289:CDS
GGCGTAATTAGAGATAAATA+TGG 0.527641 2.4:-8032559 MS.gene31289:CDS
GAAGTTCATCCTCGTTTGGT+TGG 0.528033 2.4:+8033313 None:intergenic
TCATGGCACTTAAAGGCCGT+TGG 0.528425 2.4:-8033161 MS.gene31289:CDS
GATGAGCCCAAATGGAGTGC+AGG 0.546812 2.4:-8032967 MS.gene31289:CDS
ACCAACAAGCGAATATAATC+CGG 0.551759 2.4:-8032869 MS.gene31289:CDS
GGTGTTGGTGGGTTAGAAGA+TGG 0.553851 2.4:+8032889 None:intergenic
ATTCGCTTGTTGGTGTTGGT+GGG 0.556256 2.4:+8032878 None:intergenic
CCAACAAACTTATTAACAGA+AGG 0.558467 2.4:+8033123 None:intergenic
AGCCAAAATAATGTCGCTCT+CGG 0.564239 2.4:+8033059 None:intergenic
GGATGCTACAAACAAGCCGT+TGG 0.570988 2.4:-8033102 MS.gene31289:CDS
CGCGATTATGAATATTTGTG+TGG 0.578026 2.4:-8032580 MS.gene31289:CDS
ACACCACAATCTTGTACTCA+AGG 0.582202 2.4:-8033408 MS.gene31289:CDS
TCAAAAGGCGGCTAAAAGGA+AGG 0.585709 2.4:-8032830 MS.gene31289:CDS
AGAAGATGGCGAATATGCTC+CGG 0.586086 2.4:+8032903 None:intergenic
TCTCTGGACAATTCTTGTCG+CGG 0.608642 2.4:+8033190 None:intergenic
AGCCGAGATAGAGAGGAAAA+GGG 0.609069 2.4:-8032686 MS.gene31289:CDS
CCACCTTGAGTACAAGATTG+TGG 0.623073 2.4:+8033405 None:intergenic
TGAAGAAATTGGATCCGCAT+CGG 0.629641 2.4:-8033361 MS.gene31289:CDS
CGGTGTATTTACAGCCGATG+CGG 0.630471 2.4:+8033347 None:intergenic
AATTCACATTTGAGGCTGCG+TGG 0.634228 2.4:-8033002 MS.gene31289:CDS
GGCTGCGTGGAGATTAATGA+AGG 0.645333 2.4:-8032989 MS.gene31289:CDS
AATAAGATTCCAACCAAACG+AGG 0.646389 2.4:-8033322 MS.gene31289:CDS
GGGTTGAAGATGATAGGGAA+AGG 0.648735 2.4:-8032666 MS.gene31289:CDS
TAACCCCATTATGTCTCATG+AGG 0.648921 2.4:-8033478 None:intergenic
GCGTCCAATCGGTCAAAAGG+CGG 0.659841 2.4:-8032842 MS.gene31289:CDS
CCACAATCTTGTACTCAAGG+TGG 0.693015 2.4:-8033405 MS.gene31289:CDS
GCGACAAGAATTGTCCAGAG+AGG 0.746257 2.4:-8033188 MS.gene31289:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTCAAAGTATCAATTT+TGG + chr2.4:8033243-8033262 None:intergenic 15.0%
! ATAAACCTTGATGAAGAAAT+TGG - chr2.4:8032645-8032664 MS.gene31289:CDS 25.0%
! GAAGGAAAAAGAAAAACTTA+AGG - chr2.4:8033205-8033224 MS.gene31289:CDS 25.0%
! TGATTTACAAATTCTCTTGA+AGG - chr2.4:8033385-8033404 MS.gene31289:CDS 25.0%
!! ATCAAAAAGGAGATTGTTTT+TGG - chr2.4:8032775-8032794 MS.gene31289:CDS 25.0%
!! GAGGTTCAAATTGATAATAA+TGG - chr2.4:8032558-8032577 MS.gene31289:CDS 25.0%
!!! TTCTTTGTTCTTTTTGAAGA+AGG + chr2.4:8033086-8033105 None:intergenic 25.0%
!!! TTTGGTTGGAATCTTATTTT+CGG + chr2.4:8032693-8032712 None:intergenic 25.0%
AGAACAAAGAATTCAACTTC+CGG - chr2.4:8033095-8033114 MS.gene31289:CDS 30.0%
ATCATGGTTCAACATTTCAA+AGG - chr2.4:8032734-8032753 MS.gene31289:CDS 30.0%
ATGAACTTCTCATTCAATCA+TGG - chr2.4:8032718-8032737 MS.gene31289:CDS 30.0%
ATTGTTGGGATTGATCAAAA+AGG - chr2.4:8032762-8032781 MS.gene31289:CDS 30.0%
CCAACAAACTTATTAACAGA+AGG + chr2.4:8032897-8032916 None:intergenic 30.0%
CCTTCTGTTAATAAGTTTGT+TGG - chr2.4:8032894-8032913 MS.gene31289:CDS 30.0%
GGCGTAATTAGAGATAAATA+TGG - chr2.4:8033458-8033477 MS.gene31289:CDS 30.0%
!!! ACCACTTTTTTTCAAACCAA+CGG + chr2.4:8032934-8032953 None:intergenic 30.0%
AATAAGATTCCAACCAAACG+AGG - chr2.4:8032695-8032714 MS.gene31289:CDS 35.0%
ACCAACAAGCGAATATAATC+CGG - chr2.4:8033148-8033167 MS.gene31289:CDS 35.0%
AGGAATTTCATGGCACTTAA+AGG - chr2.4:8032849-8032868 MS.gene31289:CDS 35.0%
ATTTCAAAGGACTCGATTGT+TGG - chr2.4:8032747-8032766 MS.gene31289:CDS 35.0%
CGCGATTATGAATATTTGTG+TGG - chr2.4:8033437-8033456 MS.gene31289:CDS 35.0%
GAAAAGGGTTGAAGATGATA+GGG - chr2.4:8033346-8033365 MS.gene31289:CDS 35.0%
GCGTAGAAAATTCACATTTG+AGG - chr2.4:8033007-8033026 MS.gene31289:CDS 35.0%
TTTCAAAGGACTCGATTGTT+GGG - chr2.4:8032748-8032767 MS.gene31289:CDS 35.0%
! GATTGATCGTTTTATGCCAA+CGG + chr2.4:8032875-8032894 None:intergenic 35.0%
! TTATATTCGCTTGTTGGTGT+TGG + chr2.4:8033146-8033165 None:intergenic 35.0%
!!! AAGGAGATTGTTTTTGGAAG+AGG - chr2.4:8032781-8032800 MS.gene31289:CDS 35.0%
AATGAACCTGCACTCCATTT+GGG + chr2.4:8033059-8033078 None:intergenic 40.0%
ACACCACAATCTTGTACTCA+AGG - chr2.4:8032609-8032628 MS.gene31289:CDS 40.0%
AGCCAAAATAATGTCGCTCT+CGG + chr2.4:8032961-8032980 None:intergenic 40.0%
ATGAGAAGTTCATCCTCGTT+TGG + chr2.4:8032711-8032730 None:intergenic 40.0%
CGGATCCAATTTCTTCATCA+AGG + chr2.4:8032653-8032672 None:intergenic 40.0%
GGAAAAGGGTTGAAGATGAT+AGG - chr2.4:8033345-8033364 MS.gene31289:CDS 40.0%
TAATGAACCTGCACTCCATT+TGG + chr2.4:8033060-8033079 None:intergenic 40.0%
TAATGAAGGATGAGCCCAAA+TGG - chr2.4:8033042-8033061 MS.gene31289:CDS 40.0%
TCATAATCGCGTAGTTGTCT+TGG + chr2.4:8033428-8033447 None:intergenic 40.0%
TGAAGAAATTGGATCCGCAT+CGG - chr2.4:8032656-8032675 MS.gene31289:CDS 40.0%
TGCATAATCACGTGCAAACT+CGG + chr2.4:8033294-8033313 None:intergenic 40.0%
TTGTCCAGAGAGGAATTTCA+TGG - chr2.4:8032839-8032858 MS.gene31289:CDS 40.0%
! AACCCTTTTCCTCTCTATCT+CGG + chr2.4:8033336-8033355 None:intergenic 40.0%
! TGAATTTTCTACGCATGTCG+TGG + chr2.4:8033001-8033020 None:intergenic 40.0%
!! GCCGTTGGTTTGAAAAAAAG+TGG - chr2.4:8032930-8032949 MS.gene31289:CDS 40.0%
AAGCCGAGATAGAGAGGAAA+AGG - chr2.4:8033330-8033349 MS.gene31289:CDS 45.0%
AGAAGATGGCGAATATGCTC+CGG + chr2.4:8033117-8033136 None:intergenic 45.0%
AGCCGAGATAGAGAGGAAAA+GGG - chr2.4:8033331-8033350 MS.gene31289:CDS 45.0%
AGTGCCATGAAATTCCTCTC+TGG + chr2.4:8032846-8032865 None:intergenic 45.0%
ATCTTGTACTCAAGGTGGCT+TGG - chr2.4:8032617-8032636 MS.gene31289:CDS 45.0%
CCACAATCTTGTACTCAAGG+TGG - chr2.4:8032612-8032631 MS.gene31289:CDS 45.0%
CCACCTTGAGTACAAGATTG+TGG + chr2.4:8032615-8032634 None:intergenic 45.0%
GAAGTTCATCCTCGTTTGGT+TGG + chr2.4:8032707-8032726 None:intergenic 45.0%
GCCGGATTATATTCGCTTGT+TGG + chr2.4:8033152-8033171 None:intergenic 45.0%
GGGTTGAAGATGATAGGGAA+AGG - chr2.4:8033351-8033370 MS.gene31289:CDS 45.0%
GTGAAAAAGCCGAGATAGAG+AGG - chr2.4:8033324-8033343 MS.gene31289:CDS 45.0%
TCAAAAGGCGGCTAAAAGGA+AGG - chr2.4:8033187-8033206 MS.gene31289:CDS 45.0%
TCTCTGGACAATTCTTGTCG+CGG + chr2.4:8032830-8032849 None:intergenic 45.0%
! AATTCACATTTGAGGCTGCG+TGG - chr2.4:8033015-8033034 MS.gene31289:CDS 45.0%
! CTCCGAGAGCGACATTATTT+TGG - chr2.4:8032956-8032975 MS.gene31289:CDS 45.0%
!! ATTCGCTTGTTGGTGTTGGT+GGG + chr2.4:8033142-8033161 None:intergenic 45.0%
!! TATTCGCTTGTTGGTGTTGG+TGG + chr2.4:8033143-8033162 None:intergenic 45.0%
CGGTGTATTTACAGCCGATG+CGG + chr2.4:8032673-8032692 None:intergenic 50.0%
GCGACAAGAATTGTCCAGAG+AGG - chr2.4:8032829-8032848 MS.gene31289:CDS 50.0%
GGATGCTACAAACAAGCCGT+TGG - chr2.4:8032915-8032934 MS.gene31289:CDS 50.0%
GGCTGCGTGGAGATTAATGA+AGG - chr2.4:8033028-8033047 MS.gene31289:CDS 50.0%
TCATGGCACTTAAAGGCCGT+TGG - chr2.4:8032856-8032875 MS.gene31289:CDS 50.0%
TCGGTCAAAAGGCGGCTAAA+AGG - chr2.4:8033183-8033202 MS.gene31289:CDS 50.0%
! GAAGCGTCCAATCGGTCAAA+AGG - chr2.4:8033172-8033191 MS.gene31289:CDS 50.0%
! TTAGCCGCCTTTTGACCGAT+TGG + chr2.4:8033182-8033201 None:intergenic 50.0%
!! GGTGTTGGTGGGTTAGAAGA+TGG + chr2.4:8033131-8033150 None:intergenic 50.0%
GATGAGCCCAAATGGAGTGC+AGG - chr2.4:8033050-8033069 MS.gene31289:CDS 55.0%
GCGTCCAATCGGTCAAAAGG+CGG - chr2.4:8033175-8033194 MS.gene31289:CDS 55.0%
! AATCCGGCGAAGCGTCCAAT+CGG - chr2.4:8033164-8033183 MS.gene31289:CDS 55.0%
TGACCGATTGGACGCTTCGC+CGG + chr2.4:8033170-8033189 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 8032549 8033490 8032549 ID=MS.gene31289
chr2.4 mRNA 8032549 8033490 8032549 ID=MS.gene31289.t1;Parent=MS.gene31289
chr2.4 exon 8032549 8033490 8032549 ID=MS.gene31289.t1.exon1;Parent=MS.gene31289.t1
chr2.4 CDS 8032549 8033490 8032549 ID=cds.MS.gene31289.t1;Parent=MS.gene31289.t1
Gene Sequence

>MS.gene31289

ATGTCTCATGAGGTTCAAATTGATAATAATGGTGTGCAATTAAATAATCAAGAGACTTCAACACCACAATCTTGTACTCAAGGTGGCTTGGAAACTATAAACCTTGATGAAGAAATTGGATCCGCATCGGCTGTAAATACACCGAAAATAAGATTCCAACCAAACGAGGATGAACTTCTCATTCAATCATGGTTCAACATTTCAAAGGACTCGATTGTTGGGATTGATCAAAAAGGAGATTGTTTTTGGAAGAGGATTAGTGAAGCTTATAACAAGCACCGCGACAAGAATTGTCCAGAGAGGAATTTCATGGCACTTAAAGGCCGTTGGCATAAAACGATCAATCCTTCTGTTAATAAGTTTGTTGGATGCTACAAACAAGCCGTTGGTTTGAAAAAAAGTGGTAGCTCCGAGAGCGACATTATTTTGGCTGCACATGATATTTTTCACCACGACATGCGTAGAAAATTCACATTTGAGGCTGCGTGGAGATTAATGAAGGATGAGCCCAAATGGAGTGCAGGTTCATTAGAACCTTCTTCAAAAAGAACAAAGAATTCAACTTCCGGAGCATATTCGCCATCTTCTAACCCACCAACACCAACAAGCGAATATAATCCGGCGAAGCGTCCAATCGGTCAAAAGGCGGCTAAAAGGAAGGAAAAAGAAAAACTTAAGGAAACGTCTACTACCAAAATTGATACTTTGAAAAATGACTTCAACAAAAAGTTTGAGCTAATGTCCGAGTTTGCACGTGATTATGCACGTTTTGAGAGTGAAAAAGCCGAGATAGAGAGGAAAAGGGTTGAAGATGATAGGGAAAGGTCGAAGATCAATGATTTACAAATTCTCTTGAAGGATACATCGAATATGAATCCAAGACAACTACGCGATTATGAATATTTGTGTGGCGTAATTAGAGATAAATATGGACTTAATTGA
Protein sequence

>MS.gene31289.t1

MSHEVQIDNNGVQLNNQETSTPQSCTQGGLETINLDEEIGSASAVNTPKIRFQPNEDELLIQSWFNISKDSIVGIDQKGDCFWKRISEAYNKHRDKNCPERNFMALKGRWHKTINPSVNKFVGCYKQAVGLKKSGSSESDIILAAHDIFHHDMRRKFTFEAAWRLMKDEPKWSAGSLEPSSKRTKNSTSGAYSPSSNPPTPTSEYNPAKRPIGQKAAKRKEKEKLKETSTTKIDTLKNDFNKKFELMSEFARDYARFESEKAEIERKRVEDDRERSKINDLQILLKDTSNMNPRQLRDYEYLCGVIRDKYGLN