Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31314.t1 | XP_024631650.1 | 93.1 | 174 | 12 | 0 | 7 | 180 | 1 | 174 | 2.30E-76 | 295 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31314.t1 | Q9LRW7 | 90.5 | 137 | 13 | 0 | 44 | 180 | 76 | 212 | 6.5e-72 | 271.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31314.t1 | G7IKT3 | 93.1 | 174 | 12 | 0 | 7 | 180 | 1 | 174 | 1.6e-76 | 295.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene31314 | 0.824035 | 1.00E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene31314 | 0.821256 | 4.45E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene31314 | 0.811476 | 6.87E-51 | -1.69E-46 |
| MS.gene053912 | MS.gene31314 | 0.801489 | 8.79E-49 | -1.69E-46 |
| MS.gene056422 | MS.gene31314 | 0.824472 | 7.92E-54 | -1.69E-46 |
| MS.gene057537 | MS.gene31314 | 0.805294 | 1.43E-49 | -1.69E-46 |
| MS.gene058075 | MS.gene31314 | 0.800124 | 1.67E-48 | -1.69E-46 |
| MS.gene058459 | MS.gene31314 | 0.808134 | 3.59E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene31314 | 0.816476 | 5.42E-52 | -1.69E-46 |
| MS.gene059711 | MS.gene31314 | 0.815403 | 9.41E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene31314 | 0.844724 | 6.33E-59 | -1.69E-46 |
| MS.gene060837 | MS.gene31314 | 0.801813 | 7.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTAACTGAATTTGAATT+AGG | 0.133306 | 2.3:+7754611 | None:intergenic |
| TCTTAACTGAATTTGAATTA+GGG | 0.289041 | 2.3:+7754612 | None:intergenic |
| AAGATTAGGGTTTGAGTTTG+AGG | 0.302868 | 2.3:+7754640 | None:intergenic |
| AACAACTTGCCTGGTGTTCC+TGG | 0.316889 | 2.3:+7754575 | None:intergenic |
| TTCGTTGTCATCAGTGATTT+TGG | 0.328085 | 2.3:+7754428 | None:intergenic |
| GAATTAGGGTTAGGAAGATT+AGG | 0.332160 | 2.3:+7754626 | None:intergenic |
| GTCGATGTAGCTGTAGGTTT+TGG | 0.340159 | 2.3:+7754363 | None:intergenic |
| AGAGCTTTAGGAGACCTAAC+AGG | 0.369422 | 2.3:-7754233 | MS.gene31314:CDS |
| GAAACAAGCTGGAGGTGATT+AGG | 0.369571 | 2.3:-7754307 | MS.gene31314:CDS |
| AATTAGGGTTAGGAAGATTA+GGG | 0.370819 | 2.3:+7754627 | None:intergenic |
| CATGACCCTAGTAGAGCTTT+AGG | 0.371731 | 2.3:-7754245 | MS.gene31314:CDS |
| AGAGCAATCACTTCTGATTC+CGG | 0.378986 | 2.3:+7754455 | None:intergenic |
| TGGTGCACGCAGCTCTTCTC+TGG | 0.394181 | 2.3:+7754266 | None:intergenic |
| ACTGAATTTGAATTAGGGTT+AGG | 0.405640 | 2.3:+7754617 | None:intergenic |
| AATATGCAGTTCAATCTGAT+TGG | 0.433651 | 2.3:-7754145 | MS.gene31314:CDS |
| TTTCTTTGCTTAAGCTTCCA+TGG | 0.433993 | 2.3:+7754326 | None:intergenic |
| AGGTCTCCTAAAGCTCTACT+AGG | 0.450218 | 2.3:+7754239 | None:intergenic |
| TGCAAACTTCGCACATGAAA+CGG | 0.459921 | 2.3:+7754405 | None:intergenic |
| GAAAGCACGGTGAGAAGAAG+TGG | 0.517455 | 2.3:-7754190 | MS.gene31314:CDS |
| AAGGCTCATAGTAAGATTTG+TGG | 0.535441 | 2.3:-7754122 | MS.gene31314:CDS |
| GCTGGAGGTGATTAGGAAGA+AGG | 0.538892 | 2.3:-7754300 | MS.gene31314:CDS |
| ATGTGCGAAGTTTGCAACAA+GGG | 0.539035 | 2.3:-7754398 | MS.gene31314:CDS |
| TGCAGTTCAATCTGATTGGA+AGG | 0.545468 | 2.3:-7754141 | MS.gene31314:CDS |
| GCTTAAGCAAAGAAACAAGC+TGG | 0.547884 | 2.3:-7754318 | MS.gene31314:CDS |
| GTTTGAGGTGCCTCTGAAGT+TGG | 0.548806 | 2.3:+7754655 | None:intergenic |
| CTAAAGCTCTACTAGGGTCA+TGG | 0.559412 | 2.3:+7754246 | None:intergenic |
| GACGAGGACACAATCTTCCA+TGG | 0.563267 | 2.3:-7754343 | MS.gene31314:CDS |
| ATTGTGGCACACTATTCTCC+AGG | 0.563269 | 2.3:-7754079 | None:intergenic |
| AAGAGAAAGAGAAGTCTACC+AGG | 0.573845 | 2.3:-7754593 | MS.gene31314:CDS |
| AGAAGTCTACCAGGAACACC+AGG | 0.578443 | 2.3:-7754584 | MS.gene31314:intron |
| CATGTGCGAAGTTTGCAACA+AGG | 0.579589 | 2.3:-7754399 | MS.gene31314:CDS |
| TTTGATACTTCCAACTTCAG+AGG | 0.590981 | 2.3:-7754665 | MS.gene31314:CDS |
| GTCCTCGTCGATGTAGCTGT+AGG | 0.600070 | 2.3:+7754357 | None:intergenic |
| GGTCTCCTAAAGCTCTACTA+GGG | 0.614831 | 2.3:+7754240 | None:intergenic |
| GAGCTTTAGGAGACCTAACA+GGG | 0.617009 | 2.3:-7754232 | MS.gene31314:CDS |
| AAAGAGTACAGATGTGATTG+TGG | 0.617310 | 2.3:-7754095 | MS.gene31314:CDS |
| AAGCACTTTAGTAGAAAGCA+CGG | 0.627488 | 2.3:-7754203 | MS.gene31314:CDS |
| TAAGCAAAGAAACAAGCTGG+AGG | 0.718484 | 2.3:-7754315 | MS.gene31314:CDS |
| AACCTACAGCTACATCGACG+AGG | 0.721645 | 2.3:-7754359 | MS.gene31314:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTAACTGAATTTGAATTA+GGG | + | chr2.3:7754176-7754195 | None:intergenic | 20.0% |
| ! | CTCTTAACTGAATTTGAATT+AGG | + | chr2.3:7754177-7754196 | None:intergenic | 25.0% |
| !!! | AATTGAGTTGAGAAAAAAGA+AGG | + | chr2.3:7754238-7754257 | None:intergenic | 25.0% |
| AATATGCAGTTCAATCTGAT+TGG | - | chr2.3:7754640-7754659 | MS.gene31314:CDS | 30.0% | |
| AATTAGGGTTAGGAAGATTA+GGG | + | chr2.3:7754161-7754180 | None:intergenic | 30.0% | |
| ACTGAATTTGAATTAGGGTT+AGG | + | chr2.3:7754171-7754190 | None:intergenic | 30.0% | |
| TTTCTCAACTCAATTCATCT+AGG | - | chr2.3:7754243-7754262 | MS.gene31314:CDS | 30.0% | |
| AAGCACTTTAGTAGAAAGCA+CGG | - | chr2.3:7754582-7754601 | MS.gene31314:intron | 35.0% | |
| AAGGCTCATAGTAAGATTTG+TGG | - | chr2.3:7754663-7754682 | MS.gene31314:CDS | 35.0% | |
| GAATTAGGGTTAGGAAGATT+AGG | + | chr2.3:7754162-7754181 | None:intergenic | 35.0% | |
| TTTCTTTGCTTAAGCTTCCA+TGG | + | chr2.3:7754462-7754481 | None:intergenic | 35.0% | |
| TTTGATACTTCCAACTTCAG+AGG | - | chr2.3:7754120-7754139 | MS.gene31314:CDS | 35.0% | |
| ! | AAAGAGTACAGATGTGATTG+TGG | - | chr2.3:7754690-7754709 | MS.gene31314:CDS | 35.0% |
| ! | AAGATTAGGGTTTGAGTTTG+AGG | + | chr2.3:7754148-7754167 | None:intergenic | 35.0% |
| ! | TTCGTTGTCATCAGTGATTT+TGG | + | chr2.3:7754360-7754379 | None:intergenic | 35.0% |
| !!! | AGTGCTTTTTTATCCCTGTT+AGG | + | chr2.3:7754569-7754588 | None:intergenic | 35.0% |
| AAGAGAAAGAGAAGTCTACC+AGG | - | chr2.3:7754192-7754211 | MS.gene31314:CDS | 40.0% | |
| AGAGCAATCACTTCTGATTC+CGG | + | chr2.3:7754333-7754352 | None:intergenic | 40.0% | |
| ATGTGCGAAGTTTGCAACAA+GGG | - | chr2.3:7754387-7754406 | MS.gene31314:CDS | 40.0% | |
| GCTTAAGCAAAGAAACAAGC+TGG | - | chr2.3:7754467-7754486 | MS.gene31314:intron | 40.0% | |
| TAAGCAAAGAAACAAGCTGG+AGG | - | chr2.3:7754470-7754489 | MS.gene31314:intron | 40.0% | |
| TAATAACCATGCTGCAGATC+CGG | - | chr2.3:7754311-7754330 | MS.gene31314:CDS | 40.0% | |
| TGCAAACTTCGCACATGAAA+CGG | + | chr2.3:7754383-7754402 | None:intergenic | 40.0% | |
| TGCAGTTCAATCTGATTGGA+AGG | - | chr2.3:7754644-7754663 | MS.gene31314:CDS | 40.0% | |
| AAGAAGGTGAACAACTTGCC+TGG | + | chr2.3:7754222-7754241 | None:intergenic | 45.0% | |
| AGGTCTCCTAAAGCTCTACT+AGG | + | chr2.3:7754549-7754568 | None:intergenic | 45.0% | |
| CATGACCCTAGTAGAGCTTT+AGG | - | chr2.3:7754540-7754559 | MS.gene31314:intron | 45.0% | |
| CATGTGCGAAGTTTGCAACA+AGG | - | chr2.3:7754386-7754405 | MS.gene31314:CDS | 45.0% | |
| CTAAAGCTCTACTAGGGTCA+TGG | + | chr2.3:7754542-7754561 | None:intergenic | 45.0% | |
| GAAACAAGCTGGAGGTGATT+AGG | - | chr2.3:7754478-7754497 | MS.gene31314:intron | 45.0% | |
| GGTCTCCTAAAGCTCTACTA+GGG | + | chr2.3:7754548-7754567 | None:intergenic | 45.0% | |
| ! | GTCGATGTAGCTGTAGGTTT+TGG | + | chr2.3:7754425-7754444 | None:intergenic | 45.0% |
| !! | AGAGCTTTAGGAGACCTAAC+AGG | - | chr2.3:7754552-7754571 | MS.gene31314:intron | 45.0% |
| !! | GAGCTTTAGGAGACCTAACA+GGG | - | chr2.3:7754553-7754572 | MS.gene31314:intron | 45.0% |
| AACAACTTGCCTGGTGTTCC+TGG | + | chr2.3:7754213-7754232 | None:intergenic | 50.0% | |
| AACCTACAGCTACATCGACG+AGG | - | chr2.3:7754426-7754445 | MS.gene31314:CDS | 50.0% | |
| AGAAGTCTACCAGGAACACC+AGG | - | chr2.3:7754201-7754220 | MS.gene31314:CDS | 50.0% | |
| GACGAGGACACAATCTTCCA+TGG | - | chr2.3:7754442-7754461 | MS.gene31314:CDS | 50.0% | |
| GCTGGAGGTGATTAGGAAGA+AGG | - | chr2.3:7754485-7754504 | MS.gene31314:intron | 50.0% | |
| ! | GAAAGCACGGTGAGAAGAAG+TGG | - | chr2.3:7754595-7754614 | MS.gene31314:CDS | 50.0% |
| !! | GTTTGAGGTGCCTCTGAAGT+TGG | + | chr2.3:7754133-7754152 | None:intergenic | 50.0% |
| CTGATTCCGGATCTGCAGCA+TGG | + | chr2.3:7754320-7754339 | None:intergenic | 55.0% | |
| GTCCTCGTCGATGTAGCTGT+AGG | + | chr2.3:7754431-7754450 | None:intergenic | 55.0% | |
| TGGTGCACGCAGCTCTTCTC+TGG | + | chr2.3:7754522-7754541 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 7754080 | 7754727 | 7754080 | ID=MS.gene31314 |
| chr2.3 | mRNA | 7754080 | 7754727 | 7754080 | ID=MS.gene31314.t1;Parent=MS.gene31314 |
| chr2.3 | exon | 7754585 | 7754727 | 7754585 | ID=MS.gene31314.t1.exon1;Parent=MS.gene31314.t1 |
| chr2.3 | CDS | 7754585 | 7754727 | 7754585 | ID=cds.MS.gene31314.t1;Parent=MS.gene31314.t1 |
| chr2.3 | exon | 7754080 | 7754479 | 7754080 | ID=MS.gene31314.t1.exon2;Parent=MS.gene31314.t1 |
| chr2.3 | CDS | 7754080 | 7754479 | 7754080 | ID=cds.MS.gene31314.t1;Parent=MS.gene31314.t1 |
| Gene Sequence |
| Protein sequence |
