AlfalfaGEDB | MS.gene31476

M. sativa cultivar XinJiangDaYe / MS.gene31476

KEGG: K10666
GO: -
PFAM: PF13445.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31476.t1	XP_013465083.1	97.2	143	4	0	1	143	1	143	1.80E-79	305.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31476.t1	Q6R567	52.4	124	54	4	11	129	7	130	5.2e-32	138.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31476.t1	A0A072VCD2	97.2	143	4	0	1	143	1	143	1.3e-79	305.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055815	MS.gene31476	0.954363	3.50E-112	-1.69E-46
MS.gene055816	MS.gene31476	0.92223	1.29E-88	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene032302	MS.gene31476	PPI
MS.gene36072	MS.gene31476	PPI
MS.gene34211	MS.gene31476	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31476.t1	MTR_2g090295	97.203	143	4	0	1	143	1	143	7.03e-104	296
MS.gene31476.t1	MTR_8g465910	60.000	135	47	3	1	129	1	134	1.91e-53	169
MS.gene31476.t1	MTR_2g090330	63.551	107	29	2	37	143	30	126	6.46e-43	139
MS.gene31476.t1	MTR_2g090330	63.551	107	29	2	37	143	27	123	7.59e-43	139
MS.gene31476.t1	MTR_2g090325	65.789	114	24	4	29	140	7	107	3.68e-42	139
MS.gene31476.t1	MTR_2g013550	58.889	90	37	0	37	126	24	113	3.74e-35	122
MS.gene31476.t1	MTR_4g088870	45.902	122	55	5	14	126	1	120	1.68e-29	106
MS.gene31476.t1	MTR_1g021895	52.128	94	33	3	37	128	27	110	8.24e-28	103
MS.gene31476.t1	MTR_5g019300	50.562	89	36	2	40	128	35	115	5.90e-27	100
MS.gene31476.t1	MTR_4g088875	54.839	93	37	2	38	126	29	120	1.39e-26	99.8
MS.gene31476.t1	MTR_7g093390	41.964	112	51	4	15	126	149	246	2.63e-24	97.1
MS.gene31476.t1	MTR_4g029440	38.462	130	65	4	8	129	86	208	1.40e-22	92.0
MS.gene31476.t1	MTR_4g029440	38.462	130	65	4	8	129	92	214	1.44e-22	92.0
MS.gene31476.t1	MTR_1g054260	41.573	89	50	2	40	126	87	175	2.48e-22	88.2
MS.gene31476.t1	MTR_1g054280	38.889	108	58	3	19	126	56	155	6.53e-22	88.2
MS.gene31476.t1	MTR_1g054465	40.449	89	51	2	40	126	55	143	8.45e-22	86.7
MS.gene31476.t1	MTR_1g054210	43.011	93	44	3	36	126	116	201	7.41e-20	82.4
MS.gene31476.t1	MTR_2g061230	34.653	101	59	1	29	129	96	189	1.68e-17	76.3
MS.gene31476.t1	MTR_1g015390	42.623	61	25	1	42	102	366	416	3.81e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31476.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene31476.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene31476.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene31476.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene31476.t1	AT4G28270	49.573	117	41	4	24	131	2	109	1.65e-32	114
MS.gene31476.t1	AT4G27470	55.102	98	40	3	40	134	42	138	2.88e-31	112
MS.gene31476.t1	AT1G74990	44.545	110	49	3	30	137	7	106	7.55e-28	100
MS.gene31476.t1	AT2G23780	54.945	91	29	3	40	128	26	106	2.65e-27	101
MS.gene31476.t1	AT2G23780	54.945	91	29	3	40	128	26	106	2.65e-27	101
MS.gene31476.t1	AT2G23780	54.945	91	29	3	40	128	26	106	2.65e-27	101
MS.gene31476.t1	AT1G19310	46.789	109	44	4	24	128	3	101	2.94e-26	99.0
MS.gene31476.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene31476.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene31476.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene31476.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene31476.t1	AT2G42030	43.519	108	45	4	36	138	133	229	3.13e-21	88.6
MS.gene31476.t1	AT2G44410	35.965	114	56	3	19	126	100	202	4.39e-18	79.7
MS.gene31476.t1	AT2G26350	37.895	95	43	3	8	102	299	377	4.45e-11	59.7
MS.gene31476.t1	AT2G26350	37.895	95	43	3	8	102	228	306	6.93e-11	58.9

Find 39 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTTCCATGGAATCAATT+TGG	0.249281	2.4:-10479508	None:intergenic
AAAGGCAATGATCACCAATT+AGG	0.249482	2.4:+10479806	MS.gene31476:CDS
CTTTGGAAGGCGGCGTAGTT+TGG	0.290827	2.4:-10479787	None:intergenic
GCAAGTGGTGAAGCAATTTC+TGG	0.315559	2.4:+10479557	MS.gene31476:CDS
GAGATTTCTGATTTGCATAC+TGG	0.331060	2.4:-10479731	None:intergenic
CTAGACCGCTTGGTCCTTCA+TGG	0.359817	2.4:+10479843	MS.gene31476:CDS
AAGGCAATGATCACCAATTA+GGG	0.391729	2.4:+10479807	MS.gene31476:CDS
GTGGTATGACACTCCCTAAT+TGG	0.404265	2.4:-10479820	None:intergenic
CATGAAGGACCAAGCGGTCT+AGG	0.421483	2.4:-10479842	None:intergenic
TTTGCAGCGTCAAGTGATCT+TGG	0.423758	2.4:-10479878	None:intergenic
ATTAAGCCATTTGTAAATGC+AGG	0.444845	2.4:-10479663	None:intergenic
GATCCAGTGGTCACTCTTTG+TGG	0.447780	2.4:+10479623	MS.gene31476:CDS
TAATTGGTGATCATTGCCTT+TGG	0.448612	2.4:-10479804	None:intergenic
GCTGGCCCTGCATTTACAAA+TGG	0.463384	2.4:+10479657	MS.gene31476:CDS
CTCCTCAAAATACTGATTGA+AGG	0.463468	2.4:-10479477	None:intergenic
TATGAACCAAATTGATTCCA+TGG	0.475517	2.4:+10479502	MS.gene31476:CDS
TTTGTGGTCATCTCTACTGC+TGG	0.480977	2.4:+10479639	MS.gene31476:CDS
CTTTGACTGCAACATATGCT+TGG	0.503274	2.4:+10479589	MS.gene31476:CDS
GTTTGGCCGCGGCCGTAGAG+TGG	0.505416	2.4:-10479770	None:intergenic
GAAGCAATTTCTGGTTCTAG+TGG	0.514216	2.4:+10479566	MS.gene31476:CDS
TGGTGATCATTGCCTTTGGA+AGG	0.521075	2.4:-10479800	None:intergenic
TCATCCCTTGTTCCACTCTA+CGG	0.525770	2.4:+10479758	MS.gene31476:CDS
AAGGCGGCGTAGTTTGGCCG+CGG	0.530973	2.4:-10479781	None:intergenic
CGCGGCCGTAGAGTGGAACA+AGG	0.543163	2.4:-10479763	None:intergenic
GTCATACCACCTAGACCGCT+TGG	0.564556	2.4:+10479833	MS.gene31476:CDS
CAAACTACGCCGCCTTCCAA+AGG	0.572732	2.4:+10479788	MS.gene31476:CDS
GGTAAGTTCGTCATCCATGA+AGG	0.573904	2.4:-10479857	None:intergenic
TGATCATTGCCTTTGGAAGG+CGG	0.577497	2.4:-10479797	None:intergenic
TGACCACAAAGAGTGACCAC+TGG	0.593140	2.4:-10479626	None:intergenic
TTAAGCCATTTGTAAATGCA+GGG	0.593204	2.4:-10479662	None:intergenic
GATTTGCATACTGGACATTG+TGG	0.605821	2.4:-10479722	None:intergenic
AAATCAAGAGAAGCAGAAGG+AGG	0.609520	2.4:+10479697	MS.gene31476:CDS
CGAAAATCAAGAGAAGCAGA+AGG	0.635468	2.4:+10479694	MS.gene31476:CDS
GAAGGACCAAGCGGTCTAGG+TGG	0.660331	2.4:-10479839	None:intergenic
GGAGTGTGTGCAAGATCCAG+TGG	0.671005	2.4:+10479610	MS.gene31476:CDS
CTTGTTCCACTCTACGGCCG+CGG	0.675004	2.4:+10479764	MS.gene31476:CDS
GTCATCCATGAAGGACCAAG+CGG	0.707584	2.4:-10479848	None:intergenic
GAAGTGTGGAAATGTGCAAG+TGG	0.714630	2.4:+10479542	MS.gene31476:CDS
GCGGCCGTAGAGTGGAACAA+GGG	0.719279	2.4:-10479762	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAATCATCTCAAGAAGTG+TGG	+	chr2.4:10479528-10479547	MS.gene31476:CDS	30.0%
	ATTAAGCCATTTGTAAATGC+AGG	-	chr2.4:10479666-10479685	None:intergenic	30.0%
	TTAAGCCATTTGTAAATGCA+GGG	-	chr2.4:10479665-10479684	None:intergenic	30.0%
	TTTCTTCCATGGAATCAATT+TGG	-	chr2.4:10479511-10479530	None:intergenic	30.0%
!	TATGAACCAAATTGATTCCA+TGG	+	chr2.4:10479502-10479521	MS.gene31476:CDS	30.0%
!	TTGAGATGATTTTTCTTCCA+TGG	-	chr2.4:10479522-10479541	None:intergenic	30.0%
!!	TTCAATCAGTATTTTGAGGA+GGG	+	chr2.4:10479479-10479498	MS.gene31476:CDS	30.0%
	AAAGGCAATGATCACCAATT+AGG	+	chr2.4:10479806-10479825	MS.gene31476:CDS	35.0%
	AAGGCAATGATCACCAATTA+GGG	+	chr2.4:10479807-10479826	MS.gene31476:CDS	35.0%
!	CTCCTCAAAATACTGATTGA+AGG	-	chr2.4:10479480-10479499	None:intergenic	35.0%
!	CTTCTGCTTCTCTTGATTTT+CGG	-	chr2.4:10479696-10479715	None:intergenic	35.0%
!	GAGATTTCTGATTTGCATAC+TGG	-	chr2.4:10479734-10479753	None:intergenic	35.0%
!	TAATTGGTGATCATTGCCTT+TGG	-	chr2.4:10479807-10479826	None:intergenic	35.0%
!!	CTTCAATCAGTATTTTGAGG+AGG	+	chr2.4:10479478-10479497	MS.gene31476:CDS	35.0%
	AAATCAAGAGAAGCAGAAGG+AGG	+	chr2.4:10479697-10479716	MS.gene31476:CDS	40.0%
	CGAAAATCAAGAGAAGCAGA+AGG	+	chr2.4:10479694-10479713	MS.gene31476:CDS	40.0%
	CTTTGACTGCAACATATGCT+TGG	+	chr2.4:10479589-10479608	MS.gene31476:CDS	40.0%
	GATTTGCATACTGGACATTG+TGG	-	chr2.4:10479725-10479744	None:intergenic	40.0%
!	GGCCTTCAATCAGTATTTTG+AGG	+	chr2.4:10479475-10479494	MS.gene31476:CDS	40.0%
!!	GAAGCAATTTCTGGTTCTAG+TGG	+	chr2.4:10479566-10479585	MS.gene31476:CDS	40.0%
	GAAGTGTGGAAATGTGCAAG+TGG	+	chr2.4:10479542-10479561	MS.gene31476:CDS	45.0%
	GCAAGTGGTGAAGCAATTTC+TGG	+	chr2.4:10479557-10479576	MS.gene31476:CDS	45.0%
	GGTAAGTTCGTCATCCATGA+AGG	-	chr2.4:10479860-10479879	None:intergenic	45.0%
	GTGGTATGACACTCCCTAAT+TGG	-	chr2.4:10479823-10479842	None:intergenic	45.0%
	TCATCCCTTGTTCCACTCTA+CGG	+	chr2.4:10479758-10479777	MS.gene31476:CDS	45.0%
	TGGTGATCATTGCCTTTGGA+AGG	-	chr2.4:10479803-10479822	None:intergenic	45.0%
	TTTGCAGCGTCAAGTGATCT+TGG	-	chr2.4:10479881-10479900	None:intergenic	45.0%
	TTTGTGGTCATCTCTACTGC+TGG	+	chr2.4:10479639-10479658	MS.gene31476:CDS	45.0%
!	GCTTCTCTTGATTTTCGGCA+TGG	-	chr2.4:10479691-10479710	None:intergenic	45.0%
!	TGATCATTGCCTTTGGAAGG+CGG	-	chr2.4:10479800-10479819	None:intergenic	45.0%
	GATCCAGTGGTCACTCTTTG+TGG	+	chr2.4:10479623-10479642	MS.gene31476:CDS	50.0%
	GCTGGCCCTGCATTTACAAA+TGG	+	chr2.4:10479657-10479676	MS.gene31476:CDS	50.0%
	GTCATCCATGAAGGACCAAG+CGG	-	chr2.4:10479851-10479870	None:intergenic	50.0%
	TGACCACAAAGAGTGACCAC+TGG	-	chr2.4:10479629-10479648	None:intergenic	50.0%
	CAAACTACGCCGCCTTCCAA+AGG	+	chr2.4:10479788-10479807	MS.gene31476:CDS	55.0%
	CATGAAGGACCAAGCGGTCT+AGG	-	chr2.4:10479845-10479864	None:intergenic	55.0%
	CTAGACCGCTTGGTCCTTCA+TGG	+	chr2.4:10479843-10479862	MS.gene31476:CDS	55.0%
	GGAGTGTGTGCAAGATCCAG+TGG	+	chr2.4:10479610-10479629	MS.gene31476:CDS	55.0%
	GTCATACCACCTAGACCGCT+TGG	+	chr2.4:10479833-10479852	MS.gene31476:CDS	55.0%
!	CTTTGGAAGGCGGCGTAGTT+TGG	-	chr2.4:10479790-10479809	None:intergenic	55.0%
	CTTGTTCCACTCTACGGCCG+CGG	+	chr2.4:10479764-10479783	MS.gene31476:CDS	60.0%
	GAAGGACCAAGCGGTCTAGG+TGG	-	chr2.4:10479842-10479861	None:intergenic	60.0%
	GCGGCCGTAGAGTGGAACAA+GGG	-	chr2.4:10479765-10479784	None:intergenic	60.0%
	AAGGCGGCGTAGTTTGGCCG+CGG	-	chr2.4:10479784-10479803	None:intergenic	65.0%
	CGCGGCCGTAGAGTGGAACA+AGG	-	chr2.4:10479766-10479785	None:intergenic	65.0%
	GTTTGGCCGCGGCCGTAGAG+TGG	-	chr2.4:10479773-10479792	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	10479473	10479901	10479473	ID=MS.gene31476
chr2.4	mRNA	10479473	10479901	10479473	ID=MS.gene31476.t1;Parent=MS.gene31476
chr2.4	exon	10479473	10479901	10479473	ID=MS.gene31476.t1.exon1;Parent=MS.gene31476.t1
chr2.4	CDS	10479473	10479901	10479473	ID=cds.MS.gene31476.t1;Parent=MS.gene31476.t1

Gene Sequence

>MS.gene31476

ATGGCCTTCAATCAGTATTTTGAGGAGGGTATGAACCAAATTGATTCCATGGAAGAAAAATCATCTCAAGAAGTGTGGAAATGTGCAAGTGGTGAAGCAATTTCTGGTTCTAGTGGCTTTGACTGCAACATATGCTTGGAGTGTGTGCAAGATCCAGTGGTCACTCTTTGTGGTCATCTCTACTGCTGGCCCTGCATTTACAAATGGCTTAATTTCCATGCCGAAAATCAAGAGAAGCAGAAGGAGGAGCCACAATGTCCAGTATGCAAATCAGAAATCTCAAAATCATCCCTTGTTCCACTCTACGGCCGCGGCCAAACTACGCCGCCTTCCAAAGGCAATGATCACCAATTAGGGAGTGTCATACCACCTAGACCGCTTGGTCCTTCATGGATGACGAACTTACCAAGATCACTTGACGCTGCAAAT

Protein sequence

>MS.gene31476.t1

MAFNQYFEEGMNQIDSMEEKSSQEVWKCASGEAISGSSGFDCNICLECVQDPVVTLCGHLYCWPCIYKWLNFHAENQEKQKEEPQCPVCKSEISKSSLVPLYGRGQTTPPSKGNDHQLGSVIPPRPLGPSWMTNLPRSLDAAN