AlfalfaGEDB | MS.gene055815

M. sativa cultivar XinJiangDaYe / MS.gene055815

KEGG: K10666
GO: -
PFAM: PF13445.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055815.t1	XP_013465083.1	97.2	143	4	0	1	143	1	143	1.80E-79	305.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055815.t1	Q6R567	52.4	124	54	4	11	129	7	130	5.2e-32	138.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055815.t1	A0A072VCD2	97.2	143	4	0	1	143	1	143	1.3e-79	305.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055815	MS.gene055816	0.966508	5.15E-126	-1.69E-46
MS.gene055815	MS.gene31476	0.954363	3.50E-112	-1.69E-46
MS.gene055815	MS.gene31477	0.935678	5.81E-97	-1.69E-46
MS.gene055815	MS.gene40837	0.81707	3.99E-52	-1.69E-46
MS.gene055815	MS.gene53316	0.931198	5.38E-94	-1.69E-46
MS.gene055815	MS.gene57162	0.801053	1.08E-48	-1.69E-46
MS.gene055815	MS.gene85450	0.804136	2.50E-49	-1.69E-46
MS.gene055815	MS.gene94818	0.81566	8.25E-52	-1.69E-46
MS.gene055815	MS.gene98445	0.814401	1.57E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene36072	MS.gene055815	PPI
MS.gene055815	MS.gene34211	PPI
MS.gene055815	MS.gene032302	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055815.t1	MTR_2g090295	97.203	143	4	0	1	143	1	143	7.03e-104	296
MS.gene055815.t1	MTR_8g465910	60.000	135	47	3	1	129	1	134	1.91e-53	169
MS.gene055815.t1	MTR_2g090330	63.551	107	29	2	37	143	30	126	6.46e-43	139
MS.gene055815.t1	MTR_2g090330	63.551	107	29	2	37	143	27	123	7.59e-43	139
MS.gene055815.t1	MTR_2g090325	65.789	114	24	4	29	140	7	107	3.68e-42	139
MS.gene055815.t1	MTR_2g013550	58.889	90	37	0	37	126	24	113	3.74e-35	122
MS.gene055815.t1	MTR_4g088870	45.902	122	55	5	14	126	1	120	1.68e-29	106
MS.gene055815.t1	MTR_1g021895	52.128	94	33	3	37	128	27	110	8.24e-28	103
MS.gene055815.t1	MTR_5g019300	50.562	89	36	2	40	128	35	115	5.90e-27	100
MS.gene055815.t1	MTR_4g088875	54.839	93	37	2	38	126	29	120	1.39e-26	99.8
MS.gene055815.t1	MTR_7g093390	41.964	112	51	4	15	126	149	246	2.63e-24	97.1
MS.gene055815.t1	MTR_4g029440	38.462	130	65	4	8	129	86	208	1.40e-22	92.0
MS.gene055815.t1	MTR_4g029440	38.462	130	65	4	8	129	92	214	1.44e-22	92.0
MS.gene055815.t1	MTR_1g054260	41.573	89	50	2	40	126	87	175	2.48e-22	88.2
MS.gene055815.t1	MTR_1g054280	38.889	108	58	3	19	126	56	155	6.53e-22	88.2
MS.gene055815.t1	MTR_1g054465	40.449	89	51	2	40	126	55	143	8.45e-22	86.7
MS.gene055815.t1	MTR_1g054210	43.011	93	44	3	36	126	116	201	7.41e-20	82.4
MS.gene055815.t1	MTR_2g061230	34.653	101	59	1	29	129	96	189	1.68e-17	76.3
MS.gene055815.t1	MTR_1g015390	42.623	61	25	1	42	102	366	416	3.81e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055815.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene055815.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene055815.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene055815.t1	AT4G03510	50.476	105	46	2	35	138	41	140	2.82e-33	117
MS.gene055815.t1	AT4G28270	49.573	117	41	4	24	131	2	109	1.65e-32	114
MS.gene055815.t1	AT4G27470	55.102	98	40	3	40	134	42	138	2.88e-31	112
MS.gene055815.t1	AT1G74990	44.545	110	49	3	30	137	7	106	7.55e-28	100
MS.gene055815.t1	AT2G23780	54.945	91	29	3	40	128	26	106	2.65e-27	101
MS.gene055815.t1	AT2G23780	54.945	91	29	3	40	128	26	106	2.65e-27	101
MS.gene055815.t1	AT2G23780	54.945	91	29	3	40	128	26	106	2.65e-27	101
MS.gene055815.t1	AT1G19310	46.789	109	44	4	24	128	3	101	2.94e-26	99.0
MS.gene055815.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene055815.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene055815.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene055815.t1	AT3G58030	43.137	102	45	3	27	126	122	212	1.18e-22	92.4
MS.gene055815.t1	AT2G42030	43.519	108	45	4	36	138	133	229	3.13e-21	88.6
MS.gene055815.t1	AT2G44410	35.965	114	56	3	19	126	100	202	4.39e-18	79.7
MS.gene055815.t1	AT2G26350	37.895	95	43	3	8	102	299	377	4.45e-11	59.7
MS.gene055815.t1	AT2G26350	37.895	95	43	3	8	102	228	306	6.93e-11	58.9

Find 39 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTTCCATGGAATCAATT+TGG	0.249281	2.1:-10765932	None:intergenic
AAAGGCAATGATCACCAATT+AGG	0.249482	2.1:+10766230	MS.gene055815:CDS
CTTTGGAAGGCGGCGTAGTT+TGG	0.290827	2.1:-10766211	None:intergenic
GCAAGTGGTGAAGCAATTTC+TGG	0.315559	2.1:+10765981	MS.gene055815:CDS
GAGATTTCTGATTTGCATAC+TGG	0.331060	2.1:-10766155	None:intergenic
CTAGACCGCTTGGTCCTTCA+TGG	0.359817	2.1:+10766267	MS.gene055815:CDS
AAGGCAATGATCACCAATTA+GGG	0.391729	2.1:+10766231	MS.gene055815:CDS
GTGGTATGACACTCCCTAAT+TGG	0.404265	2.1:-10766244	None:intergenic
CATGAAGGACCAAGCGGTCT+AGG	0.421483	2.1:-10766266	None:intergenic
TTTGCAGCGTCAAGTGATCT+TGG	0.423758	2.1:-10766302	None:intergenic
ATTAAGCCATTTGTAAATGC+AGG	0.444845	2.1:-10766087	None:intergenic
GATCCAGTGGTCACTCTTTG+TGG	0.447780	2.1:+10766047	MS.gene055815:CDS
TAATTGGTGATCATTGCCTT+TGG	0.448612	2.1:-10766228	None:intergenic
GCTGGCCCTGCATTTACAAA+TGG	0.463384	2.1:+10766081	MS.gene055815:CDS
CTCCTCAAAATACTGATTGA+AGG	0.463468	2.1:-10765901	None:intergenic
TATGAACCAAATTGATTCCA+TGG	0.475517	2.1:+10765926	MS.gene055815:CDS
TTTGTGGTCATCTCTACTGC+TGG	0.480977	2.1:+10766063	MS.gene055815:CDS
CTTTGACTGCAACATATGCT+TGG	0.503274	2.1:+10766013	MS.gene055815:CDS
GTTTGGCCGCGGCCGTAGAG+TGG	0.505416	2.1:-10766194	None:intergenic
GAAGCAATTTCTGGTTCTAG+TGG	0.514216	2.1:+10765990	MS.gene055815:CDS
TGGTGATCATTGCCTTTGGA+AGG	0.521075	2.1:-10766224	None:intergenic
TCATCCCTTGTTCCACTCTA+CGG	0.525770	2.1:+10766182	MS.gene055815:CDS
AAGGCGGCGTAGTTTGGCCG+CGG	0.530973	2.1:-10766205	None:intergenic
CGCGGCCGTAGAGTGGAACA+AGG	0.543163	2.1:-10766187	None:intergenic
GTCATACCACCTAGACCGCT+TGG	0.564556	2.1:+10766257	MS.gene055815:CDS
CAAACTACGCCGCCTTCCAA+AGG	0.572732	2.1:+10766212	MS.gene055815:CDS
GGTAAGTTCGTCATCCATGA+AGG	0.573904	2.1:-10766281	None:intergenic
TGATCATTGCCTTTGGAAGG+CGG	0.577497	2.1:-10766221	None:intergenic
TGACCACAAAGAGTGACCAC+TGG	0.593140	2.1:-10766050	None:intergenic
TTAAGCCATTTGTAAATGCA+GGG	0.593204	2.1:-10766086	None:intergenic
GATTTGCATACTGGACATTG+TGG	0.605821	2.1:-10766146	None:intergenic
AAATCAAGAGAAGCAGAAGG+AGG	0.609520	2.1:+10766121	MS.gene055815:CDS
CGAAAATCAAGAGAAGCAGA+AGG	0.635468	2.1:+10766118	MS.gene055815:CDS
GAAGGACCAAGCGGTCTAGG+TGG	0.660331	2.1:-10766263	None:intergenic
GGAGTGTGTGCAAGATCCAG+TGG	0.671005	2.1:+10766034	MS.gene055815:CDS
CTTGTTCCACTCTACGGCCG+CGG	0.675004	2.1:+10766188	MS.gene055815:CDS
GTCATCCATGAAGGACCAAG+CGG	0.707584	2.1:-10766272	None:intergenic
GAAGTGTGGAAATGTGCAAG+TGG	0.714630	2.1:+10765966	MS.gene055815:CDS
GCGGCCGTAGAGTGGAACAA+GGG	0.719279	2.1:-10766186	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAATCATCTCAAGAAGTG+TGG	+	chr2.1:10765952-10765971	MS.gene055815:CDS	30.0%
	ATTAAGCCATTTGTAAATGC+AGG	-	chr2.1:10766090-10766109	None:intergenic	30.0%
	TTAAGCCATTTGTAAATGCA+GGG	-	chr2.1:10766089-10766108	None:intergenic	30.0%
	TTTCTTCCATGGAATCAATT+TGG	-	chr2.1:10765935-10765954	None:intergenic	30.0%
!	TATGAACCAAATTGATTCCA+TGG	+	chr2.1:10765926-10765945	MS.gene055815:CDS	30.0%
!	TTGAGATGATTTTTCTTCCA+TGG	-	chr2.1:10765946-10765965	None:intergenic	30.0%
!!	TTCAATCAGTATTTTGAGGA+GGG	+	chr2.1:10765903-10765922	MS.gene055815:CDS	30.0%
	AAAGGCAATGATCACCAATT+AGG	+	chr2.1:10766230-10766249	MS.gene055815:CDS	35.0%
	AAGGCAATGATCACCAATTA+GGG	+	chr2.1:10766231-10766250	MS.gene055815:CDS	35.0%
!	CTCCTCAAAATACTGATTGA+AGG	-	chr2.1:10765904-10765923	None:intergenic	35.0%
!	CTTCTGCTTCTCTTGATTTT+CGG	-	chr2.1:10766120-10766139	None:intergenic	35.0%
!	GAGATTTCTGATTTGCATAC+TGG	-	chr2.1:10766158-10766177	None:intergenic	35.0%
!	TAATTGGTGATCATTGCCTT+TGG	-	chr2.1:10766231-10766250	None:intergenic	35.0%
!!	CTTCAATCAGTATTTTGAGG+AGG	+	chr2.1:10765902-10765921	MS.gene055815:CDS	35.0%
	AAATCAAGAGAAGCAGAAGG+AGG	+	chr2.1:10766121-10766140	MS.gene055815:CDS	40.0%
	CGAAAATCAAGAGAAGCAGA+AGG	+	chr2.1:10766118-10766137	MS.gene055815:CDS	40.0%
	CTTTGACTGCAACATATGCT+TGG	+	chr2.1:10766013-10766032	MS.gene055815:CDS	40.0%
	GATTTGCATACTGGACATTG+TGG	-	chr2.1:10766149-10766168	None:intergenic	40.0%
!	GGCCTTCAATCAGTATTTTG+AGG	+	chr2.1:10765899-10765918	MS.gene055815:CDS	40.0%
!!	GAAGCAATTTCTGGTTCTAG+TGG	+	chr2.1:10765990-10766009	MS.gene055815:CDS	40.0%
	GAAGTGTGGAAATGTGCAAG+TGG	+	chr2.1:10765966-10765985	MS.gene055815:CDS	45.0%
	GCAAGTGGTGAAGCAATTTC+TGG	+	chr2.1:10765981-10766000	MS.gene055815:CDS	45.0%
	GGTAAGTTCGTCATCCATGA+AGG	-	chr2.1:10766284-10766303	None:intergenic	45.0%
	GTGGTATGACACTCCCTAAT+TGG	-	chr2.1:10766247-10766266	None:intergenic	45.0%
	TCATCCCTTGTTCCACTCTA+CGG	+	chr2.1:10766182-10766201	MS.gene055815:CDS	45.0%
	TGGTGATCATTGCCTTTGGA+AGG	-	chr2.1:10766227-10766246	None:intergenic	45.0%
	TTTGCAGCGTCAAGTGATCT+TGG	-	chr2.1:10766305-10766324	None:intergenic	45.0%
	TTTGTGGTCATCTCTACTGC+TGG	+	chr2.1:10766063-10766082	MS.gene055815:CDS	45.0%
!	GCTTCTCTTGATTTTCGGCA+TGG	-	chr2.1:10766115-10766134	None:intergenic	45.0%
!	TGATCATTGCCTTTGGAAGG+CGG	-	chr2.1:10766224-10766243	None:intergenic	45.0%
	GATCCAGTGGTCACTCTTTG+TGG	+	chr2.1:10766047-10766066	MS.gene055815:CDS	50.0%
	GCTGGCCCTGCATTTACAAA+TGG	+	chr2.1:10766081-10766100	MS.gene055815:CDS	50.0%
	GTCATCCATGAAGGACCAAG+CGG	-	chr2.1:10766275-10766294	None:intergenic	50.0%
	TGACCACAAAGAGTGACCAC+TGG	-	chr2.1:10766053-10766072	None:intergenic	50.0%
	CAAACTACGCCGCCTTCCAA+AGG	+	chr2.1:10766212-10766231	MS.gene055815:CDS	55.0%
	CATGAAGGACCAAGCGGTCT+AGG	-	chr2.1:10766269-10766288	None:intergenic	55.0%
	CTAGACCGCTTGGTCCTTCA+TGG	+	chr2.1:10766267-10766286	MS.gene055815:CDS	55.0%
	GGAGTGTGTGCAAGATCCAG+TGG	+	chr2.1:10766034-10766053	MS.gene055815:CDS	55.0%
	GTCATACCACCTAGACCGCT+TGG	+	chr2.1:10766257-10766276	MS.gene055815:CDS	55.0%
!	CTTTGGAAGGCGGCGTAGTT+TGG	-	chr2.1:10766214-10766233	None:intergenic	55.0%
	CTTGTTCCACTCTACGGCCG+CGG	+	chr2.1:10766188-10766207	MS.gene055815:CDS	60.0%
	GAAGGACCAAGCGGTCTAGG+TGG	-	chr2.1:10766266-10766285	None:intergenic	60.0%
	GCGGCCGTAGAGTGGAACAA+GGG	-	chr2.1:10766189-10766208	None:intergenic	60.0%
	AAGGCGGCGTAGTTTGGCCG+CGG	-	chr2.1:10766208-10766227	None:intergenic	65.0%
	CGCGGCCGTAGAGTGGAACA+AGG	-	chr2.1:10766190-10766209	None:intergenic	65.0%
	GTTTGGCCGCGGCCGTAGAG+TGG	-	chr2.1:10766197-10766216	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	10765897	10766325	10765897	ID=MS.gene055815
chr2.1	mRNA	10765897	10766325	10765897	ID=MS.gene055815.t1;Parent=MS.gene055815
chr2.1	exon	10765897	10766325	10765897	ID=MS.gene055815.t1.exon1;Parent=MS.gene055815.t1
chr2.1	CDS	10765897	10766325	10765897	ID=cds.MS.gene055815.t1;Parent=MS.gene055815.t1

Gene Sequence

>MS.gene055815

ATGGCCTTCAATCAGTATTTTGAGGAGGGTATGAACCAAATTGATTCCATGGAAGAAAAATCATCTCAAGAAGTGTGGAAATGTGCAAGTGGTGAAGCAATTTCTGGTTCTAGTGGCTTTGACTGCAACATATGCTTGGAGTGTGTGCAAGATCCAGTGGTCACTCTTTGTGGTCATCTCTACTGCTGGCCCTGCATTTACAAATGGCTTAATTTCCATGCCGAAAATCAAGAGAAGCAGAAGGAGGAGCCACAATGTCCAGTATGCAAATCAGAAATCTCAAAATCATCCCTTGTTCCACTCTACGGCCGCGGCCAAACTACGCCGCCTTCCAAAGGCAATGATCACCAATTAGGGAGTGTCATACCACCTAGACCGCTTGGTCCTTCATGGATGACGAACTTACCAAGATCACTTGACGCTGCAAAT

Protein sequence

>MS.gene055815.t1

MAFNQYFEEGMNQIDSMEEKSSQEVWKCASGEAISGSSGFDCNICLECVQDPVVTLCGHLYCWPCIYKWLNFHAENQEKQKEEPQCPVCKSEISKSSLVPLYGRGQTTPPSKGNDHQLGSVIPPRPLGPSWMTNLPRSLDAAN