Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32207.t1 | XP_003608243.1 | 92.8 | 470 | 34 | 0 | 1 | 470 | 59 | 528 | 5.50E-263 | 916.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32207.t1 | Q9FZC5 | 55.9 | 465 | 199 | 4 | 4 | 466 | 65 | 525 | 5.2e-153 | 542.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32207.t1 | Q2HTY4 | 92.8 | 470 | 34 | 0 | 1 | 470 | 59 | 528 | 3.9e-263 | 916.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051699 | MS.gene32207 | 0.804259 | 2.35E-49 | -1.69E-46 |
| MS.gene052559 | MS.gene32207 | 0.95706 | 6.73E-115 | -1.69E-46 |
| MS.gene052560 | MS.gene32207 | 0.909704 | 4.24E-82 | -1.69E-46 |
| MS.gene055964 | MS.gene32207 | 0.808758 | 2.65E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene32207 | 0.808797 | 2.60E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene32207 | 0.805636 | 1.21E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene32207 | 0.81884 | 1.59E-52 | -1.69E-46 |
| MS.gene06023 | MS.gene32207 | 0.826646 | 2.42E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32207.t1 | MTR_4g091200 | 92.766 | 470 | 34 | 0 | 1 | 470 | 59 | 528 | 0.0 | 894 |
| MS.gene32207.t1 | MTR_6g017205 | 91.239 | 468 | 41 | 0 | 1 | 468 | 59 | 526 | 0.0 | 870 |
| MS.gene32207.t1 | MTR_4g057990 | 82.090 | 469 | 80 | 2 | 1 | 469 | 59 | 523 | 0.0 | 780 |
| MS.gene32207.t1 | MTR_4g091190 | 80.342 | 468 | 90 | 1 | 1 | 468 | 63 | 528 | 0.0 | 768 |
| MS.gene32207.t1 | MTR_4g088355 | 78.891 | 469 | 99 | 0 | 1 | 469 | 66 | 534 | 0.0 | 760 |
| MS.gene32207.t1 | MTR_2g023590 | 79.787 | 470 | 92 | 2 | 1 | 469 | 1 | 468 | 0.0 | 752 |
| MS.gene32207.t1 | MTR_4g091170 | 73.203 | 459 | 105 | 1 | 10 | 468 | 102 | 542 | 0.0 | 707 |
| MS.gene32207.t1 | MTR_4g091150 | 63.462 | 468 | 169 | 2 | 1 | 466 | 71 | 538 | 0.0 | 598 |
| MS.gene32207.t1 | MTR_2g031560 | 54.506 | 466 | 209 | 2 | 3 | 467 | 59 | 522 | 0.0 | 545 |
| MS.gene32207.t1 | MTR_2g031590 | 53.503 | 471 | 210 | 4 | 3 | 467 | 64 | 531 | 2.16e-179 | 513 |
| MS.gene32207.t1 | MTR_4g094498 | 53.763 | 465 | 211 | 3 | 3 | 467 | 73 | 533 | 5.78e-174 | 500 |
| MS.gene32207.t1 | MTR_2g031390 | 50.321 | 467 | 224 | 6 | 4 | 466 | 65 | 527 | 1.59e-166 | 481 |
| MS.gene32207.t1 | MTR_4g094488 | 53.700 | 473 | 210 | 4 | 3 | 468 | 67 | 537 | 3.82e-165 | 477 |
| MS.gene32207.t1 | MTR_4g094495 | 52.331 | 472 | 216 | 4 | 4 | 468 | 71 | 540 | 3.40e-164 | 475 |
| MS.gene32207.t1 | MTR_4g094492 | 50.959 | 469 | 224 | 3 | 4 | 467 | 68 | 535 | 1.25e-163 | 473 |
| MS.gene32207.t1 | MTR_4g094520 | 51.812 | 469 | 215 | 6 | 4 | 467 | 69 | 531 | 1.27e-163 | 473 |
| MS.gene32207.t1 | MTR_4g094480 | 51.599 | 469 | 222 | 3 | 4 | 468 | 68 | 535 | 3.87e-161 | 467 |
| MS.gene32207.t1 | MTR_3g009820 | 48.707 | 464 | 231 | 5 | 4 | 466 | 63 | 520 | 3.90e-161 | 466 |
| MS.gene32207.t1 | MTR_4g094478 | 52.350 | 468 | 218 | 3 | 4 | 467 | 68 | 534 | 7.78e-161 | 466 |
| MS.gene32207.t1 | MTR_4g094518 | 50.533 | 469 | 218 | 7 | 5 | 466 | 67 | 528 | 2.59e-160 | 464 |
| MS.gene32207.t1 | MTR_2g031490 | 51.489 | 470 | 218 | 6 | 3 | 466 | 71 | 536 | 6.50e-159 | 461 |
| MS.gene32207.t1 | MTR_2g031510 | 50.105 | 475 | 217 | 6 | 3 | 466 | 73 | 538 | 2.28e-157 | 457 |
| MS.gene32207.t1 | MTR_2g031420 | 51.282 | 468 | 221 | 4 | 3 | 469 | 67 | 528 | 4.69e-155 | 451 |
| MS.gene32207.t1 | MTR_2g031460 | 50.431 | 464 | 223 | 4 | 3 | 465 | 67 | 524 | 2.91e-154 | 449 |
| MS.gene32207.t1 | MTR_3g009880 | 48.707 | 464 | 231 | 5 | 4 | 466 | 61 | 518 | 3.90e-154 | 449 |
| MS.gene32207.t1 | MTR_3g009760 | 47.414 | 464 | 237 | 5 | 4 | 466 | 63 | 520 | 8.54e-154 | 447 |
| MS.gene32207.t1 | MTR_4g094418 | 51.259 | 437 | 205 | 4 | 40 | 469 | 1 | 436 | 2.76e-153 | 449 |
| MS.gene32207.t1 | MTR_4g094515 | 51.592 | 471 | 219 | 6 | 3 | 468 | 74 | 540 | 4.72e-150 | 457 |
| MS.gene32207.t1 | MTR_4g094515 | 51.709 | 468 | 219 | 5 | 3 | 467 | 686 | 1149 | 2.37e-149 | 455 |
| MS.gene32207.t1 | MTR_1g076830 | 38.559 | 472 | 274 | 8 | 3 | 465 | 68 | 532 | 1.00e-101 | 315 |
| MS.gene32207.t1 | MTR_2g031600 | 65.000 | 180 | 63 | 0 | 3 | 182 | 65 | 244 | 2.07e-71 | 232 |
| MS.gene32207.t1 | MTR_2g031600 | 51.875 | 160 | 76 | 1 | 308 | 467 | 241 | 399 | 7.75e-54 | 186 |
| MS.gene32207.t1 | MTR_3g009850 | 48.214 | 224 | 113 | 3 | 244 | 466 | 5 | 226 | 1.55e-64 | 208 |
| MS.gene32207.t1 | MTR_2g084370 | 46.847 | 111 | 37 | 2 | 64 | 174 | 1 | 89 | 5.49e-29 | 110 |
| MS.gene32207.t1 | MTR_2g084405 | 45.570 | 79 | 43 | 0 | 64 | 142 | 1 | 79 | 5.03e-16 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32207.t1 | AT4G20800 | 55.579 | 466 | 204 | 2 | 4 | 469 | 64 | 526 | 1.35e-178 | 511 |
| MS.gene32207.t1 | AT1G26420 | 55.172 | 464 | 202 | 4 | 4 | 466 | 65 | 523 | 1.50e-176 | 506 |
| MS.gene32207.t1 | AT1G26390 | 55.914 | 465 | 199 | 4 | 4 | 466 | 65 | 525 | 2.49e-175 | 503 |
| MS.gene32207.t1 | AT1G26380 | 55.388 | 464 | 201 | 4 | 4 | 466 | 65 | 523 | 9.16e-175 | 501 |
| MS.gene32207.t1 | AT1G30720 | 53.632 | 468 | 212 | 3 | 2 | 468 | 63 | 526 | 1.51e-172 | 495 |
| MS.gene32207.t1 | AT1G34575 | 54.818 | 467 | 199 | 4 | 3 | 466 | 65 | 522 | 2.54e-172 | 495 |
| MS.gene32207.t1 | AT1G30710 | 54.661 | 472 | 199 | 7 | 3 | 469 | 68 | 529 | 3.19e-171 | 492 |
| MS.gene32207.t1 | AT1G26400 | 53.632 | 468 | 211 | 6 | 4 | 470 | 65 | 527 | 2.33e-170 | 490 |
| MS.gene32207.t1 | AT1G30730 | 52.350 | 468 | 218 | 3 | 2 | 468 | 62 | 525 | 4.94e-170 | 489 |
| MS.gene32207.t1 | AT1G26410 | 55.172 | 464 | 202 | 5 | 4 | 466 | 89 | 547 | 5.17e-169 | 487 |
| MS.gene32207.t1 | AT1G30700 | 52.137 | 468 | 215 | 5 | 3 | 466 | 62 | 524 | 3.42e-164 | 474 |
| MS.gene32207.t1 | AT5G44400 | 50.107 | 467 | 225 | 6 | 4 | 466 | 72 | 534 | 2.26e-160 | 465 |
| MS.gene32207.t1 | AT5G44390 | 50.746 | 469 | 223 | 5 | 4 | 467 | 74 | 539 | 6.31e-159 | 461 |
| MS.gene32207.t1 | AT2G34790 | 50.000 | 468 | 224 | 5 | 4 | 468 | 70 | 530 | 2.77e-158 | 459 |
| MS.gene32207.t1 | AT2G34810 | 49.787 | 470 | 230 | 3 | 3 | 467 | 70 | 538 | 3.13e-157 | 457 |
| MS.gene32207.t1 | AT4G20840 | 51.277 | 470 | 216 | 7 | 3 | 467 | 73 | 534 | 5.27e-157 | 456 |
| MS.gene32207.t1 | AT1G30760 | 50.000 | 468 | 222 | 6 | 4 | 467 | 72 | 531 | 4.55e-155 | 451 |
| MS.gene32207.t1 | AT1G30760 | 50.000 | 468 | 222 | 6 | 4 | 467 | 90 | 549 | 8.25e-155 | 451 |
| MS.gene32207.t1 | AT1G11770 | 49.254 | 469 | 227 | 6 | 3 | 467 | 67 | 528 | 3.27e-151 | 441 |
| MS.gene32207.t1 | AT1G01980 | 51.158 | 475 | 214 | 9 | 3 | 467 | 67 | 533 | 2.71e-148 | 434 |
| MS.gene32207.t1 | AT5G44380 | 48.712 | 466 | 233 | 4 | 4 | 466 | 75 | 537 | 2.93e-147 | 431 |
| MS.gene32207.t1 | AT5G44380 | 48.712 | 466 | 233 | 4 | 4 | 466 | 115 | 577 | 6.04e-147 | 432 |
| MS.gene32207.t1 | AT4G20860 | 48.026 | 456 | 231 | 4 | 12 | 466 | 77 | 527 | 1.77e-146 | 429 |
| MS.gene32207.t1 | AT4G20820 | 47.854 | 466 | 229 | 5 | 3 | 466 | 71 | 524 | 1.90e-145 | 426 |
| MS.gene32207.t1 | AT5G44360 | 48.394 | 467 | 229 | 7 | 4 | 466 | 86 | 544 | 1.35e-142 | 420 |
| MS.gene32207.t1 | AT5G44360 | 48.394 | 467 | 229 | 7 | 4 | 466 | 70 | 528 | 1.38e-142 | 419 |
| MS.gene32207.t1 | AT4G20830 | 49.574 | 470 | 224 | 7 | 3 | 467 | 74 | 535 | 8.89e-142 | 417 |
| MS.gene32207.t1 | AT4G20830 | 49.574 | 470 | 224 | 7 | 3 | 467 | 74 | 535 | 3.59e-141 | 417 |
| MS.gene32207.t1 | AT5G44440 | 46.383 | 470 | 239 | 5 | 3 | 466 | 65 | 527 | 5.62e-139 | 410 |
| MS.gene32207.t1 | AT5G44440 | 46.383 | 470 | 239 | 5 | 3 | 466 | 135 | 597 | 8.98e-138 | 409 |
| MS.gene32207.t1 | AT5G44410 | 48.832 | 471 | 222 | 9 | 3 | 466 | 69 | 527 | 9.86e-136 | 402 |
| MS.gene32207.t1 | AT1G30740 | 47.872 | 470 | 234 | 7 | 3 | 467 | 64 | 527 | 5.05e-132 | 392 |
| MS.gene32207.t1 | AT1G30740 | 47.872 | 470 | 234 | 7 | 3 | 467 | 67 | 530 | 5.48e-132 | 392 |
Find 85 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCTAATGTTGTTCAAAT+TGG | 0.228780 | 4.4:-22696466 | MS.gene32207:CDS |
| AAGCTGAATGTTGTTGCTTT+TGG | 0.230709 | 4.4:+22697038 | None:intergenic |
| GGAAATTTGATGAGAAAATT+TGG | 0.258916 | 4.4:-22696766 | MS.gene32207:CDS |
| TGGCACATTTAACTGTTTCT+TGG | 0.267359 | 4.4:+22697058 | None:intergenic |
| AAACTTGCTTCCATATGTTT+TGG | 0.269184 | 4.4:+22695850 | None:intergenic |
| AATAGATCTTTATGCAGTTT+TGG | 0.273624 | 4.4:+22696499 | None:intergenic |
| AATCATTAGACCACGAATTA+AGG | 0.276835 | 4.4:+22696029 | None:intergenic |
| AGTAAGGAATTCCCCGAATT+AGG | 0.285169 | 4.4:-22696364 | MS.gene32207:CDS |
| AACAAGCTTGCTTTCCCATC+TGG | 0.299204 | 4.4:-22696838 | MS.gene32207:CDS |
| TGACCAATTTGAACAACATT+AGG | 0.312133 | 4.4:+22696463 | None:intergenic |
| TCTTTCATCTTTCCACCATA+TGG | 0.316468 | 4.4:+22696112 | None:intergenic |
| ATAGATTCTAGAGCTTCTTT+AGG | 0.318699 | 4.4:+22696181 | None:intergenic |
| TCTAGAGCTTCTTTAGGAAT+AGG | 0.321024 | 4.4:+22696187 | None:intergenic |
| ACGTTGGTTCAAACCTAATT+CGG | 0.321901 | 4.4:+22696351 | None:intergenic |
| GGTGGTGGTGGTGCTAGTTT+TGG | 0.331271 | 4.4:-22696643 | MS.gene32207:CDS |
| AAGAAGCTCTAGAATCTATA+TGG | 0.334429 | 4.4:-22696177 | MS.gene32207:CDS |
| TTTGACATTGCCAAAACATA+TGG | 0.339181 | 4.4:-22695860 | MS.gene32207:CDS |
| CGTTGGTTCAAACCTAATTC+GGG | 0.357298 | 4.4:+22696352 | None:intergenic |
| GGGAGGCATTCCTCAATTAT+AGG | 0.357483 | 4.4:-22695925 | MS.gene32207:CDS |
| CATTTCTCTGGTGGTGGGTA+TGG | 0.360817 | 4.4:-22696787 | MS.gene32207:CDS |
| GTTATAATCGCCAAAGGTTT+CGG | 0.368910 | 4.4:+22697102 | None:intergenic |
| AATTATAGGGATGCTGATAT+TGG | 0.376157 | 4.4:-22695911 | MS.gene32207:CDS |
| GTAGTGCAAGTTAGTTTCAT+TGG | 0.379275 | 4.4:-22696427 | MS.gene32207:CDS |
| TCAAATCTCGTTACATTACT+TGG | 0.393941 | 4.4:+22695878 | None:intergenic |
| ACGATAACCTGTGGAGTTAT+CGG | 0.397449 | 4.4:+22696589 | None:intergenic |
| TAAATTGGTTGAGGATCCTT+TGG | 0.399190 | 4.4:+22696241 | None:intergenic |
| TACTCCTCTTGAAGCATTGT+TGG | 0.402290 | 4.4:-22696269 | MS.gene32207:CDS |
| GTGATTGCCTTTCAATGCCT+TGG | 0.404900 | 4.4:-22696330 | MS.gene32207:CDS |
| GGTGGTCATTTCTCTGGTGG+TGG | 0.426644 | 4.4:-22696793 | MS.gene32207:CDS |
| TCAACAGCATGGGTTGAATC+AGG | 0.427451 | 4.4:-22696904 | MS.gene32207:CDS |
| ACAAGCTTGCTTTCCCATCT+GGG | 0.436569 | 4.4:-22696837 | MS.gene32207:CDS |
| TCTTTCATGGAAACTTCAAT+TGG | 0.441584 | 4.4:-22696614 | MS.gene32207:CDS |
| GGAGGCATTCCTCAATTATA+GGG | 0.443623 | 4.4:-22695924 | MS.gene32207:CDS |
| GAAATTCTCAGGATCAACTT+TGG | 0.444017 | 4.4:+22695790 | None:intergenic |
| CTTATGCAATGGAATCCATA+TGG | 0.450449 | 4.4:-22696127 | MS.gene32207:CDS |
| TATTCTTGATAGAAAATCAA+TGG | 0.458066 | 4.4:-22696692 | MS.gene32207:CDS |
| GGTGCTGGTGGTCATTTCTC+TGG | 0.459504 | 4.4:-22696799 | MS.gene32207:CDS |
| TCTGGGGTCTGCTTTACTCT+AGG | 0.464774 | 4.4:-22696820 | MS.gene32207:CDS |
| AGCAACAACACCGAAACCTT+TGG | 0.466175 | 4.4:-22697112 | MS.gene32207:CDS |
| CTCGGAGAATTCGAAACATA+AGG | 0.468696 | 4.4:+22695947 | None:intergenic |
| GTCTGCTTTACTCTAGGTGC+TGG | 0.472600 | 4.4:-22696814 | MS.gene32207:CDS |
| CATATGGATTCCATTGCATA+AGG | 0.477148 | 4.4:+22696128 | None:intergenic |
| TGATTCAATACCTTAATTCG+TGG | 0.477340 | 4.4:-22696039 | MS.gene32207:CDS |
| CAACTGATGTTGTTTACAAA+TGG | 0.485694 | 4.4:-22696534 | MS.gene32207:CDS |
| TCATATCTAAAGAAATTCTC+AGG | 0.490816 | 4.4:+22695779 | None:intergenic |
| GTGGTCATTTCTCTGGTGGT+GGG | 0.501321 | 4.4:-22696792 | MS.gene32207:CDS |
| AGAGTAAAGCAGACCCCAGA+TGG | 0.503056 | 4.4:+22696823 | None:intergenic |
| AACAAGTCAAGTATAACAAA+AGG | 0.504620 | 4.4:+22696961 | None:intergenic |
| AACAAGTTTCCTACTCTATG+AGG | 0.510558 | 4.4:+22696064 | None:intergenic |
| TGCTTTACTCTAGGTGCTGG+TGG | 0.512366 | 4.4:-22696811 | MS.gene32207:CDS |
| TGGGCTATAAGAGGTGGTGG+TGG | 0.518497 | 4.4:-22696655 | MS.gene32207:CDS |
| TTTCCTACTCTATGAGGAAA+CGG | 0.519543 | 4.4:+22696070 | None:intergenic |
| GCTTCAAGAGGAGTACCAAT+TGG | 0.520632 | 4.4:+22696277 | None:intergenic |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 4.4:-22696691 | MS.gene32207:CDS |
| GTGAGAATTTCCTTATGCAA+TGG | 0.526102 | 4.4:-22696138 | MS.gene32207:CDS |
| GTTGTTCAAATTGGTCAAGA+GGG | 0.530168 | 4.4:-22696457 | MS.gene32207:CDS |
| ATTGGTTCCGATAACTCCAC+AGG | 0.531147 | 4.4:-22696596 | MS.gene32207:CDS |
| ATATGGAAATTGATGATTGA+AGG | 0.537491 | 4.4:-22696160 | MS.gene32207:CDS |
| TATCAGCATCCCTATAATTG+AGG | 0.547264 | 4.4:+22695915 | None:intergenic |
| GTTGAATCAGGTGCAACACT+TGG | 0.555249 | 4.4:-22696892 | MS.gene32207:CDS |
| TGTTGTTCAAATTGGTCAAG+AGG | 0.558808 | 4.4:-22696458 | MS.gene32207:CDS |
| AGCATTGTTGGAAGAACCAA+AGG | 0.559301 | 4.4:-22696257 | MS.gene32207:CDS |
| GCTGGTGGTCATTTCTCTGG+TGG | 0.564457 | 4.4:-22696796 | MS.gene32207:CDS |
| CAAGCTTGCTTTCCCATCTG+GGG | 0.567506 | 4.4:-22696836 | MS.gene32207:CDS |
| AATTAATCCAAGGCATTGAA+AGG | 0.570542 | 4.4:+22696323 | None:intergenic |
| AGTGGCGGTCACGACTATGA+AGG | 0.571849 | 4.4:-22697006 | MS.gene32207:CDS |
| TTTACAAGAATCAACAGCAT+GGG | 0.572516 | 4.4:-22696914 | MS.gene32207:CDS |
| TAATTGAGGAATGCCTCCCT+CGG | 0.575705 | 4.4:+22695929 | None:intergenic |
| TGAGGAAACGGTGTTTCTGA+TGG | 0.577426 | 4.4:+22696082 | None:intergenic |
| TTTGCAGTTATAATCGCCAA+AGG | 0.586317 | 4.4:+22697096 | None:intergenic |
| TCTTCCAACAATGCTTCAAG+AGG | 0.594711 | 4.4:+22696265 | None:intergenic |
| ACACCGTTTCCTCATAGAGT+AGG | 0.600052 | 4.4:-22696073 | MS.gene32207:CDS |
| AAAAGAGTGGAATTAATCCA+AGG | 0.600902 | 4.4:+22696313 | None:intergenic |
| TTATGTTTCGAATTCTCCGA+GGG | 0.609571 | 4.4:-22695945 | MS.gene32207:CDS |
| ATTTACAAGAATCAACAGCA+TGG | 0.616536 | 4.4:-22696915 | MS.gene32207:CDS |
| TGTTTCGAATTCTCCGAGGG+AGG | 0.634404 | 4.4:-22695942 | MS.gene32207:CDS |
| GAGTAAAGCAGACCCCAGAT+GGG | 0.637967 | 4.4:+22696824 | None:intergenic |
| CAGCTTAGAATTCGAAGTGG+CGG | 0.641139 | 4.4:-22697021 | MS.gene32207:CDS |
| ATGCAATGGAATCCATATGG+TGG | 0.646403 | 4.4:-22696124 | MS.gene32207:CDS |
| GCATTGAAAGGCAATCACGT+TGG | 0.665080 | 4.4:+22696335 | None:intergenic |
| ACATCGAAAACGATAACCTG+TGG | 0.672224 | 4.4:+22696580 | None:intergenic |
| CTTATGTTTCGAATTCTCCG+AGG | 0.672859 | 4.4:-22695946 | MS.gene32207:CDS |
| GTTGGTTCAAACCTAATTCG+GGG | 0.693085 | 4.4:+22696353 | None:intergenic |
| ATTCAGCTTAGAATTCGAAG+TGG | 0.701549 | 4.4:-22697024 | MS.gene32207:CDS |
| GTGAAAAGGAATGTCAGTGA+AGG | 0.713631 | 4.4:-22696559 | MS.gene32207:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTATTTTTTTGATAAAT+TGG | + | chr4.4:22696669-22696688 | None:intergenic | 10.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | - | chr4.4:22696201-22696220 | MS.gene32207:CDS | 20.0% |
| !! | TATTCTTGATAGAAAATCAA+TGG | - | chr4.4:22696200-22696219 | MS.gene32207:CDS | 20.0% |
| !!! | ACTTTTAATTTTGATGAGTA+AGG | - | chr4.4:22696512-22696531 | MS.gene32207:CDS | 20.0% |
| !!! | TTTTTTTGATAAATTGGTTG+AGG | + | chr4.4:22696663-22696682 | None:intergenic | 20.0% |
| ! | AACAAGTCAAGTATAACAAA+AGG | + | chr4.4:22695934-22695953 | None:intergenic | 25.0% |
| ! | GCAAAAATTGTTGATGTTAA+GGG | - | chr4.4:22696174-22696193 | MS.gene32207:CDS | 25.0% |
| ! | GGAAATTTGATGAGAAAATT+TGG | - | chr4.4:22696126-22696145 | MS.gene32207:CDS | 25.0% |
| ! | TCATATCTAAAGAAATTCTC+AGG | + | chr4.4:22697116-22697135 | None:intergenic | 25.0% |
| ! | TGCAAAAATTGTTGATGTTA+AGG | - | chr4.4:22696173-22696192 | MS.gene32207:CDS | 25.0% |
| !! | AATAGATCTTTATGCAGTTT+TGG | + | chr4.4:22696396-22696415 | None:intergenic | 25.0% |
| !! | AGTTTCATTGGTCAATTTTT+AGG | - | chr4.4:22696477-22696496 | MS.gene32207:CDS | 25.0% |
| !! | ATATGGAAATTGATGATTGA+AGG | - | chr4.4:22696732-22696751 | MS.gene32207:CDS | 25.0% |
| AAAAGAGTGGAATTAATCCA+AGG | + | chr4.4:22696582-22696601 | None:intergenic | 30.0% | |
| AATCATTAGACCACGAATTA+AGG | + | chr4.4:22696866-22696885 | None:intergenic | 30.0% | |
| AATTATAGGGATGCTGATAT+TGG | - | chr4.4:22696981-22697000 | MS.gene32207:CDS | 30.0% | |
| ATTTACAAGAATCAACAGCA+TGG | - | chr4.4:22695977-22695996 | MS.gene32207:CDS | 30.0% | |
| CAACCTAATGTTGTTCAAAT+TGG | - | chr4.4:22696426-22696445 | MS.gene32207:CDS | 30.0% | |
| TCAAATCTCGTTACATTACT+TGG | + | chr4.4:22697017-22697036 | None:intergenic | 30.0% | |
| TCTTTCATGGAAACTTCAAT+TGG | - | chr4.4:22696278-22696297 | MS.gene32207:CDS | 30.0% | |
| TGACCAATTTGAACAACATT+AGG | + | chr4.4:22696432-22696451 | None:intergenic | 30.0% | |
| TGATTCAATACCTTAATTCG+TGG | - | chr4.4:22696853-22696872 | MS.gene32207:CDS | 30.0% | |
| TTTACAAGAATCAACAGCAT+GGG | - | chr4.4:22695978-22695997 | MS.gene32207:CDS | 30.0% | |
| TTTGACATTGCCAAAACATA+TGG | - | chr4.4:22697032-22697051 | MS.gene32207:CDS | 30.0% | |
| ! | CAACTGATGTTGTTTACAAA+TGG | - | chr4.4:22696358-22696377 | MS.gene32207:CDS | 30.0% |
| ! | TTATCGTTTTCGATGTGAAA+AGG | - | chr4.4:22696319-22696338 | MS.gene32207:CDS | 30.0% |
| !! | AAACTTGCTTCCATATGTTT+TGG | + | chr4.4:22697045-22697064 | None:intergenic | 30.0% |
| !! | AAGAAGCTCTAGAATCTATA+TGG | - | chr4.4:22696715-22696734 | MS.gene32207:CDS | 30.0% |
| !! | AATTAATCCAAGGCATTGAA+AGG | + | chr4.4:22696572-22696591 | None:intergenic | 30.0% |
| !! | ATAGATTCTAGAGCTTCTTT+AGG | + | chr4.4:22696714-22696733 | None:intergenic | 30.0% |
| !! | TATGGAAGCAAGTTTTTCAA+AGG | - | chr4.4:22697050-22697069 | MS.gene32207:CDS | 30.0% |
| !!! | AATGGGAGAAGATCTTTTTT+GGG | - | chr4.4:22696218-22696237 | MS.gene32207:CDS | 30.0% |
| !!! | AATTCCACTCTTTTTTGGTA+TGG | - | chr4.4:22696588-22696607 | MS.gene32207:CDS | 30.0% |
| !!! | GGATTAATTCCACTCTTTTT+TGG | - | chr4.4:22696583-22696602 | MS.gene32207:CDS | 30.0% |
| !!! | GTTTTGGTGTTATTCTTTCA+TGG | - | chr4.4:22696265-22696284 | MS.gene32207:CDS | 30.0% |
| AACAAGTTTCCTACTCTATG+AGG | + | chr4.4:22696831-22696850 | None:intergenic | 35.0% | |
| AATTGGTCAAGAGGGAAAAA+AGG | - | chr4.4:22696443-22696462 | MS.gene32207:CDS | 35.0% | |
| ATTCAGCTTAGAATTCGAAG+TGG | - | chr4.4:22695868-22695887 | MS.gene32207:CDS | 35.0% | |
| CATATGGATTCCATTGCATA+AGG | + | chr4.4:22696767-22696786 | None:intergenic | 35.0% | |
| CTTATGCAATGGAATCCATA+TGG | - | chr4.4:22696765-22696784 | MS.gene32207:CDS | 35.0% | |
| GAAATTCTCAGGATCAACTT+TGG | + | chr4.4:22697105-22697124 | None:intergenic | 35.0% | |
| GTGAGAATTTCCTTATGCAA+TGG | - | chr4.4:22696754-22696773 | MS.gene32207:CDS | 35.0% | |
| GTTATAATCGCCAAAGGTTT+CGG | + | chr4.4:22695793-22695812 | None:intergenic | 35.0% | |
| GTTGTTCAAATTGGTCAAGA+GGG | - | chr4.4:22696435-22696454 | MS.gene32207:CDS | 35.0% | |
| TAAATTGGTTGAGGATCCTT+TGG | + | chr4.4:22696654-22696673 | None:intergenic | 35.0% | |
| TATCAGCATCCCTATAATTG+AGG | + | chr4.4:22696980-22696999 | None:intergenic | 35.0% | |
| TCTTTCATCTTTCCACCATA+TGG | + | chr4.4:22696783-22696802 | None:intergenic | 35.0% | |
| TGTTGTTCAAATTGGTCAAG+AGG | - | chr4.4:22696434-22696453 | MS.gene32207:CDS | 35.0% | |
| TTATGTTTCGAATTCTCCGA+GGG | - | chr4.4:22696947-22696966 | MS.gene32207:CDS | 35.0% | |
| TTTCCTACTCTATGAGGAAA+CGG | + | chr4.4:22696825-22696844 | None:intergenic | 35.0% | |
| TTTGCAGTTATAATCGCCAA+AGG | + | chr4.4:22695799-22695818 | None:intergenic | 35.0% | |
| ! | GTAGTGCAAGTTAGTTTCAT+TGG | - | chr4.4:22696465-22696484 | MS.gene32207:CDS | 35.0% |
| ! | TCTAGAGCTTCTTTAGGAAT+AGG | + | chr4.4:22696708-22696727 | None:intergenic | 35.0% |
| ! | TGGCACATTTAACTGTTTCT+TGG | + | chr4.4:22695837-22695856 | None:intergenic | 35.0% |
| !! | ACGTTGGTTCAAACCTAATT+CGG | + | chr4.4:22696544-22696563 | None:intergenic | 35.0% |
| !!! | AAGCTGAATGTTGTTGCTTT+TGG | + | chr4.4:22695857-22695876 | None:intergenic | 35.0% |
| !!! | CAATGGGAGAAGATCTTTTT+TGG | - | chr4.4:22696217-22696236 | MS.gene32207:CDS | 35.0% |
| !!! | CTTGTTGCTTTTGTTTGCAA+TGG | + | chr4.4:22696040-22696059 | None:intergenic | 35.0% |
| !!! | GATCTTTTTTGGGCTATAAG+AGG | - | chr4.4:22696228-22696247 | MS.gene32207:CDS | 35.0% |
| ACATCGAAAACGATAACCTG+TGG | + | chr4.4:22696315-22696334 | None:intergenic | 40.0% | |
| ACGATAACCTGTGGAGTTAT+CGG | + | chr4.4:22696306-22696325 | None:intergenic | 40.0% | |
| AGCATTGTTGGAAGAACCAA+AGG | - | chr4.4:22696635-22696654 | MS.gene32207:CDS | 40.0% | |
| AGTAAGGAATTCCCCGAATT+AGG | - | chr4.4:22696528-22696547 | MS.gene32207:CDS | 40.0% | |
| ATGCAATGGAATCCATATGG+TGG | - | chr4.4:22696768-22696787 | MS.gene32207:CDS | 40.0% | |
| CTCGGAGAATTCGAAACATA+AGG | + | chr4.4:22696948-22696967 | None:intergenic | 40.0% | |
| CTGACCATACCAAAAAAGAG+TGG | + | chr4.4:22696595-22696614 | None:intergenic | 40.0% | |
| CTTATGTTTCGAATTCTCCG+AGG | - | chr4.4:22696946-22696965 | MS.gene32207:CDS | 40.0% | |
| GTGAAAAGGAATGTCAGTGA+AGG | - | chr4.4:22696333-22696352 | MS.gene32207:CDS | 40.0% | |
| TCTTCCAACAATGCTTCAAG+AGG | + | chr4.4:22696630-22696649 | None:intergenic | 40.0% | |
| ! | GTTGGTTCAAACCTAATTCG+GGG | + | chr4.4:22696542-22696561 | None:intergenic | 40.0% |
| ! | TTTTGGTATGGTCAGCCAAT+TGG | - | chr4.4:22696600-22696619 | MS.gene32207:CDS | 40.0% |
| !! | CGTTGGTTCAAACCTAATTC+GGG | + | chr4.4:22696543-22696562 | None:intergenic | 40.0% |
| !! | GGAGGCATTCCTCAATTATA+GGG | - | chr4.4:22696968-22696987 | MS.gene32207:CDS | 40.0% |
| !! | TACTCCTCTTGAAGCATTGT+TGG | - | chr4.4:22696623-22696642 | MS.gene32207:CDS | 40.0% |
| !!! | CTTTTTTGGGCTATAAGAGG+TGG | - | chr4.4:22696231-22696250 | MS.gene32207:CDS | 40.0% |
| ACACCGTTTCCTCATAGAGT+AGG | - | chr4.4:22696819-22696838 | MS.gene32207:CDS | 45.0% | |
| AGCAACAACACCGAAACCTT+TGG | - | chr4.4:22695780-22695799 | MS.gene32207:CDS | 45.0% | |
| CAGCTTAGAATTCGAAGTGG+CGG | - | chr4.4:22695871-22695890 | MS.gene32207:CDS | 45.0% | |
| GCATTGAAAGGCAATCACGT+TGG | + | chr4.4:22696560-22696579 | None:intergenic | 45.0% | |
| GTGATTGCCTTTCAATGCCT+TGG | - | chr4.4:22696562-22696581 | MS.gene32207:CDS | 45.0% | |
| GTTGAATCAGGTGCAACACT+TGG | - | chr4.4:22696000-22696019 | MS.gene32207:CDS | 45.0% | |
| TAATTGAGGAATGCCTCCCT+CGG | + | chr4.4:22696966-22696985 | None:intergenic | 45.0% | |
| ! | AACAAGCTTGCTTTCCCATC+TGG | - | chr4.4:22696054-22696073 | MS.gene32207:CDS | 45.0% |
| ! | ACAAGCTTGCTTTCCCATCT+GGG | - | chr4.4:22696055-22696074 | MS.gene32207:CDS | 45.0% |
| ! | ATTGGTTCCGATAACTCCAC+AGG | - | chr4.4:22696296-22696315 | MS.gene32207:CDS | 45.0% |
| ! | TCAACAGCATGGGTTGAATC+AGG | - | chr4.4:22695988-22696007 | MS.gene32207:CDS | 45.0% |
| ! | TGAGGAAACGGTGTTTCTGA+TGG | + | chr4.4:22696813-22696832 | None:intergenic | 45.0% |
| ! | TTTTGGGCTATAAGAGGTGG+TGG | - | chr4.4:22696234-22696253 | MS.gene32207:CDS | 45.0% |
| !! | GCTTCAAGAGGAGTACCAAT+TGG | + | chr4.4:22696618-22696637 | None:intergenic | 45.0% |
| !! | GGGAGGCATTCCTCAATTAT+AGG | - | chr4.4:22696967-22696986 | MS.gene32207:CDS | 45.0% |
| AGAGTAAAGCAGACCCCAGA+TGG | + | chr4.4:22696072-22696091 | None:intergenic | 50.0% | |
| GAGTAAAGCAGACCCCAGAT+GGG | + | chr4.4:22696071-22696090 | None:intergenic | 50.0% | |
| GTCTGCTTTACTCTAGGTGC+TGG | - | chr4.4:22696078-22696097 | MS.gene32207:CDS | 50.0% | |
| GTGGTCATTTCTCTGGTGGT+GGG | - | chr4.4:22696100-22696119 | MS.gene32207:CDS | 50.0% | |
| TGTTTCGAATTCTCCGAGGG+AGG | - | chr4.4:22696950-22696969 | MS.gene32207:CDS | 50.0% | |
| ! | CAAGCTTGCTTTCCCATCTG+GGG | - | chr4.4:22696056-22696075 | MS.gene32207:CDS | 50.0% |
| ! | TCTGGGGTCTGCTTTACTCT+AGG | - | chr4.4:22696072-22696091 | MS.gene32207:CDS | 50.0% |
| !! | CATTTCTCTGGTGGTGGGTA+TGG | - | chr4.4:22696105-22696124 | MS.gene32207:CDS | 50.0% |
| !! | TGCTTTACTCTAGGTGCTGG+TGG | - | chr4.4:22696081-22696100 | MS.gene32207:CDS | 50.0% |
| AGTGGCGGTCACGACTATGA+AGG | - | chr4.4:22695886-22695905 | MS.gene32207:CDS | 55.0% | |
| GCTGGTGGTCATTTCTCTGG+TGG | - | chr4.4:22696096-22696115 | MS.gene32207:CDS | 55.0% | |
| GGTGGTCATTTCTCTGGTGG+TGG | - | chr4.4:22696099-22696118 | MS.gene32207:CDS | 55.0% | |
| ! | TGGGCTATAAGAGGTGGTGG+TGG | - | chr4.4:22696237-22696256 | MS.gene32207:CDS | 55.0% |
| !! | GGTGCTGGTGGTCATTTCTC+TGG | - | chr4.4:22696093-22696112 | MS.gene32207:CDS | 55.0% |
| !!! | GGTGGTGGTGGTGCTAGTTT+TGG | - | chr4.4:22696249-22696268 | MS.gene32207:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 22695751 | 22697163 | 22695751 | ID=MS.gene32207 |
| chr4.4 | mRNA | 22695751 | 22697163 | 22695751 | ID=MS.gene32207.t1;Parent=MS.gene32207 |
| chr4.4 | exon | 22695751 | 22697163 | 22695751 | ID=MS.gene32207.t1.exon1;Parent=MS.gene32207.t1 |
| chr4.4 | CDS | 22695751 | 22697163 | 22695751 | ID=cds.MS.gene32207.t1;Parent=MS.gene32207.t1 |
| Gene Sequence |
| Protein sequence |
