Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32569.t1 | XP_003616397.1 | 99 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 2.10E-223 | 784.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32569.t1 | Q9Y312 | 28.8 | 400 | 245 | 9 | 1 | 386 | 6 | 379 | 3.3e-36 | 154.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32569.t1 | G7KBQ2 | 99.0 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 1.5e-223 | 784.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene033017 | MS.gene32569 | PPI |
| MS.gene68979 | MS.gene32569 | PPI |
| MS.gene038800 | MS.gene32569 | PPI |
| MS.gene044498 | MS.gene32569 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32569.t1 | MTR_5g079770 | 98.977 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 0.0 | 800 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32569.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene32569.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene32569.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene32569.t1 | AT1G66510 | 66.006 | 353 | 108 | 5 | 45 | 390 | 1 | 348 | 9.29e-174 | 489 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGGTAATTGTTCGTAAA+TGG | 0.186934 | 5.1:+61986782 | MS.gene32569:intron |
| CACTATTGTTGGATGATTCT+TGG | 0.258501 | 5.1:+61990438 | MS.gene32569:CDS |
| AAGCTATTAGAGAGCAATCT+AGG | 0.318940 | 5.1:+61991091 | MS.gene32569:CDS |
| ACTTTACTGACAAAAGATTA+TGG | 0.324623 | 5.1:+61988857 | MS.gene32569:CDS |
| GTTTGCATTTATCGCCCTTA+TGG | 0.327929 | 5.1:+61988913 | MS.gene32569:CDS |
| CAGCAGAACAACGCTGTTGA+TGG | 0.346353 | 5.1:+61991124 | MS.gene32569:CDS |
| TCCTTGGTTAGCCTTCTGTT+TGG | 0.383792 | 5.1:+61989049 | MS.gene32569:CDS |
| ATTGGATTCTTTATTGATGC+TGG | 0.406561 | 5.1:+61985443 | MS.gene32569:CDS |
| ATTGTTTACCTGAGTATCAA+TGG | 0.419994 | 5.1:-61983416 | None:intergenic |
| ATGGAATTTGACCGACAACT+TGG | 0.429503 | 5.1:+61987580 | MS.gene32569:CDS |
| CTAGATGGGTCTGTCATTGA+TGG | 0.429928 | 5.1:+61990878 | MS.gene32569:CDS |
| ATCTTAGCCACTATGAAGAT+TGG | 0.447384 | 5.1:+61987611 | MS.gene32569:CDS |
| TAGTAGACAAAATGAGTTCC+CGG | 0.450062 | 5.1:-61984684 | None:intergenic |
| TGTTCGTAAATGGGACCAAC+AGG | 0.453079 | 5.1:+61986792 | MS.gene32569:CDS |
| GTTGGCAAGTTGCAGAGAAA+AGG | 0.455685 | 5.1:+61987877 | MS.gene32569:CDS |
| GCAGGTAATTGTTCGTAAAT+GGG | 0.457276 | 5.1:+61986783 | MS.gene32569:intron |
| ATCTATTATCAACTAAAATA+CGG | 0.457832 | 5.1:+61990383 | MS.gene32569:CDS |
| GGTTCTGAAGACTTGCTCCT+TGG | 0.470331 | 5.1:+61988881 | MS.gene32569:CDS |
| TCATAGTGGCTAAGATTGTA+AGG | 0.472984 | 5.1:-61987604 | None:intergenic |
| TGACTAGCCGTTCCTCCTGT+TGG | 0.475000 | 5.1:-61986807 | None:intergenic |
| AACACTGACTTCTCCTCCAA+TGG | 0.477819 | 5.1:-61987761 | None:intergenic |
| AGAAAATATACTTACTGCTT+CGG | 0.477936 | 5.1:-61989077 | None:intergenic |
| GATAATTCTGCAACATCTGT+TGG | 0.478381 | 5.1:+61987859 | MS.gene32569:CDS |
| CTAGGATGAAAGATATAGTC+TGG | 0.482454 | 5.1:+61987546 | MS.gene32569:intron |
| GGGTATTAAGATGATTCCTC+CGG | 0.487283 | 5.1:+61984665 | MS.gene32569:CDS |
| ATTTCTCCAGTGGAAATCCT+TGG | 0.497323 | 5.1:+61989033 | MS.gene32569:CDS |
| GTGTCTAGTGCTTTCTCCAT+TGG | 0.503391 | 5.1:-61987823 | None:intergenic |
| GGCTTCCTGCAGAGATGGAA+AGG | 0.504025 | 5.1:+61985406 | MS.gene32569:intron |
| GATTGTAAGGCCCAAGTTGT+CGG | 0.506606 | 5.1:-61987591 | None:intergenic |
| TATTGATGCTGGCCCTTCAG+AGG | 0.511306 | 5.1:+61985454 | MS.gene32569:CDS |
| TAAATAATACCTTACAATGA+TGG | 0.519108 | 5.1:-61990484 | None:intergenic |
| TTGGCAAGTTGCAGAGAAAA+GGG | 0.521046 | 5.1:+61987878 | MS.gene32569:CDS |
| TATGGATTCTCAAACAGCGT+TGG | 0.521890 | 5.1:+61983333 | None:intergenic |
| GATCAAATCAAACCTGGTTG+AGG | 0.524670 | 5.1:-61984712 | None:intergenic |
| AGTCAAAGTATCTGAGGAAG+AGG | 0.525053 | 5.1:+61986825 | MS.gene32569:CDS |
| TCATCTAACATCTCTACTAC+AGG | 0.528298 | 5.1:-61991268 | None:intergenic |
| TAACACAGGACCACTATTGT+TGG | 0.533398 | 5.1:+61990427 | MS.gene32569:CDS |
| CCTGAGTATCAATGGCAACA+AGG | 0.534352 | 5.1:-61983408 | None:intergenic |
| TGGAATTTGACCGACAACTT+GGG | 0.538353 | 5.1:+61987581 | MS.gene32569:CDS |
| ACACTGACTTCTCCTCCAAT+GGG | 0.539146 | 5.1:-61987760 | None:intergenic |
| CAAGAATCATCCAACAATAG+TGG | 0.539982 | 5.1:-61990437 | None:intergenic |
| CCTTGTTGCCATTGATACTC+AGG | 0.546257 | 5.1:+61983408 | MS.gene32569:CDS |
| GGCTAACCAAGGATTTCCAC+TGG | 0.549600 | 5.1:-61989039 | None:intergenic |
| ATCTTAATACCCTTAAAAGA+AGG | 0.553188 | 5.1:-61984654 | None:intergenic |
| TATTTATGAGCAGAGCCCAT+TGG | 0.553357 | 5.1:+61987745 | MS.gene32569:intron |
| CCACGTGTTGTAAAATGCAA+GGG | 0.556834 | 5.1:+61987919 | MS.gene32569:CDS |
| TTATGAGCAGAGCCCATTGG+AGG | 0.557137 | 5.1:+61987748 | MS.gene32569:intron |
| ATGCAAGGGGATCAGTGGAC+AGG | 0.557605 | 5.1:+61987933 | MS.gene32569:CDS |
| AGCTATTAGAGAGCAATCTA+GGG | 0.559485 | 5.1:+61991092 | MS.gene32569:CDS |
| TCCACGTGTTGTAAAATGCA+AGG | 0.560327 | 5.1:+61987918 | MS.gene32569:CDS |
| AAGAGCATCATCGAACGGCT+TGG | 0.567694 | 5.1:+61987658 | MS.gene32569:CDS |
| ATCGTTTCCAATCTTCATAG+TGG | 0.569850 | 5.1:-61987618 | None:intergenic |
| CGTTAGAAGCATTTCTCCAG+TGG | 0.577996 | 5.1:+61989023 | MS.gene32569:CDS |
| TCAATAAAGAATCCAATCAT+CGG | 0.579744 | 5.1:-61985437 | None:intergenic |
| TCTACTATAGTTCCTCAACC+AGG | 0.581273 | 5.1:+61984700 | MS.gene32569:CDS |
| CTGAGTATCAATGGCAACAA+GGG | 0.594931 | 5.1:-61983407 | None:intergenic |
| CTTCGGTGCAGCCAAACAGA+AGG | 0.598687 | 5.1:-61989060 | None:intergenic |
| AAACGCTCCTAAAACACCAA+TGG | 0.600774 | 5.1:+61987807 | MS.gene32569:CDS |
| TATTAGAGAGCAATCTAGGG+TGG | 0.601228 | 5.1:+61991095 | MS.gene32569:CDS |
| AGCACTAGACACGCAGCTGA+AGG | 0.603110 | 5.1:+61987834 | MS.gene32569:CDS |
| AATGGGACCAACAGGAGGAA+CGG | 0.609015 | 5.1:+61986800 | MS.gene32569:CDS |
| GGTATTAAGATGATTCCTCC+GGG | 0.612119 | 5.1:+61984666 | MS.gene32569:CDS |
| TGGCAACAAGGGTGTACTGA+GGG | 0.612425 | 5.1:-61983396 | None:intergenic |
| GTAAAATGCAAGGGGATCAG+TGG | 0.615109 | 5.1:+61987928 | MS.gene32569:CDS |
| ATTAGAGAGCAATCTAGGGT+GGG | 0.617313 | 5.1:+61991096 | MS.gene32569:CDS |
| ATGCAAACTGCAGTTCTCCA+AGG | 0.619224 | 5.1:-61988898 | None:intergenic |
| TTACAAAGAGCATCATCGAA+CGG | 0.632571 | 5.1:+61987653 | MS.gene32569:CDS |
| AGCCTTTCCACACACGAACA+CGG | 0.635124 | 5.1:+61989808 | MS.gene32569:intron |
| TAAATAGCCGTGTTCGTGTG+TGG | 0.637252 | 5.1:-61989815 | None:intergenic |
| CGGACTACAGAAAGATCGCA+AGG | 0.640325 | 5.1:+61990403 | MS.gene32569:CDS |
| ACGGCTAGTCAAAGTATCTG+AGG | 0.646658 | 5.1:+61986819 | MS.gene32569:CDS |
| CACGTGTTGTAAAATGCAAG+GGG | 0.646735 | 5.1:+61987920 | MS.gene32569:CDS |
| TACTTCTTTGAATCTTGACA+AGG | 0.647721 | 5.1:+61987960 | MS.gene32569:CDS |
| AGCCGTGTTCGTGTGTGGAA+AGG | 0.649513 | 5.1:-61989810 | None:intergenic |
| GCCAAACAGAAGGCTAACCA+AGG | 0.675388 | 5.1:-61989050 | None:intergenic |
| GTACTTTGATGAGAACGATG+AGG | 0.679398 | 5.1:+61991150 | MS.gene32569:CDS |
| TCGTAAATGGGACCAACAGG+AGG | 0.680154 | 5.1:+61986795 | MS.gene32569:CDS |
| TTACTGACAAAAGATTATGG+AGG | 0.688252 | 5.1:+61988860 | MS.gene32569:CDS |
| ATGGCAACAAGGGTGTACTG+AGG | 0.692263 | 5.1:-61983397 | None:intergenic |
| AAAGATCGCAAGGATAACAC+AGG | 0.694703 | 5.1:+61990413 | MS.gene32569:CDS |
| ACGAACACGGCTATTTACCA+AGG | 0.703530 | 5.1:+61989821 | MS.gene32569:CDS |
| GGGTGTACTGAGGGACATCG+AGG | 0.709510 | 5.1:-61983387 | None:intergenic |
| TAGACAAAATGAGTTCCCGG+AGG | 0.766673 | 5.1:-61984681 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 61983334 | 61991309 | 61983334 | ID=MS.gene32569 |
| chr5.1 | mRNA | 61983334 | 61991309 | 61983334 | ID=MS.gene32569.t1;Parent=MS.gene32569 |
| chr5.1 | exon | 61983334 | 61983429 | 61983334 | ID=MS.gene32569.t1.exon1;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61983334 | 61983429 | 61983334 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61984639 | 61984721 | 61984639 | ID=MS.gene32569.t1.exon2;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61984639 | 61984721 | 61984639 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61985418 | 61985475 | 61985418 | ID=MS.gene32569.t1.exon3;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61985418 | 61985475 | 61985418 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61986787 | 61986846 | 61986787 | ID=MS.gene32569.t1.exon4;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61986787 | 61986846 | 61986787 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61987550 | 61987679 | 61987550 | ID=MS.gene32569.t1.exon5;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61987550 | 61987679 | 61987550 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61987758 | 61987981 | 61987758 | ID=MS.gene32569.t1.exon6;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61987758 | 61987981 | 61987758 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61988842 | 61988934 | 61988842 | ID=MS.gene32569.t1.exon7;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61988842 | 61988934 | 61988842 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61989013 | 61989084 | 61989013 | ID=MS.gene32569.t1.exon8;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61989013 | 61989084 | 61989013 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61989810 | 61989842 | 61989810 | ID=MS.gene32569.t1.exon9;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61989810 | 61989842 | 61989810 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61990371 | 61990496 | 61990371 | ID=MS.gene32569.t1.exon10;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61990371 | 61990496 | 61990371 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61990860 | 61990916 | 61990860 | ID=MS.gene32569.t1.exon11;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61990860 | 61990916 | 61990860 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61991076 | 61991171 | 61991076 | ID=MS.gene32569.t1.exon12;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61991076 | 61991171 | 61991076 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| chr5.1 | exon | 61991262 | 61991309 | 61991262 | ID=MS.gene32569.t1.exon13;Parent=MS.gene32569.t1 |
| chr5.1 | CDS | 61991262 | 61991309 | 61991262 | ID=cds.MS.gene32569.t1;Parent=MS.gene32569.t1 |
| Gene Sequence |
| Protein sequence |
