AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32730.t1 MTR_3g105610 93.061 735 47 1 1 731 256 990 0.0 1395
MS.gene32730.t1 MTR_3g105595 83.037 731 117 3 1 727 141 868 0.0 1219
MS.gene32730.t1 MTR_8g073210 39.557 632 354 12 1 621 248 862 1.63e-141 439
MS.gene32730.t1 MTR_3g115910 37.042 683 394 15 1 674 247 902 2.29e-140 435
MS.gene32730.t1 MTR_3g115910 37.042 683 394 15 1 674 292 947 1.03e-139 435
MS.gene32730.t1 MTR_8g073490 37.229 693 392 17 1 671 247 918 1.22e-135 424
MS.gene32730.t1 MTR_2g088450 37.799 627 367 12 1 620 154 764 2.57e-133 414
MS.gene32730.t1 MTR_2g088450 37.799 627 367 12 1 620 187 797 1.00e-132 414
MS.gene32730.t1 MTR_2g088450 37.799 627 367 12 1 620 252 862 4.19e-132 414
MS.gene32730.t1 MTR_5g024350 35.918 632 380 14 1 622 247 863 1.84e-128 405
MS.gene32730.t1 MTR_5g024350 35.918 632 380 14 1 622 279 895 4.57e-128 405
MS.gene32730.t1 MTR_4g087925 34.609 653 373 12 1 647 266 870 4.85e-121 385
MS.gene32730.t1 MTR_2g088430 34.340 629 388 13 1 620 245 857 1.03e-119 381
MS.gene32730.t1 MTR_2g015270 33.429 700 389 21 1 676 263 909 3.02e-113 367
MS.gene32730.t1 MTR_3g115910 37.477 547 313 11 1 538 247 773 1.23e-111 356
MS.gene32730.t1 MTR_5g059920 34.548 631 351 16 1 617 264 846 5.65e-106 345
MS.gene32730.t1 MTR_5g059900 33.974 624 349 15 1 617 170 737 8.98e-100 324
MS.gene32730.t1 MTR_2g015260 30.876 719 385 23 1 696 72 701 2.23e-98 319
MS.gene32730.t1 MTR_0018s0240 31.854 631 349 16 1 617 191 754 1.87e-94 312
MS.gene32730.t1 MTR_2g088450 39.474 456 258 9 1 450 252 695 3.49e-93 305
MS.gene32730.t1 MTR_0018s0230 30.684 629 357 14 1 617 169 730 3.22e-90 300
MS.gene32730.t1 MTR_2g015290 31.587 630 356 21 1 620 259 823 3.30e-90 303
MS.gene32730.t1 MTR_2g015310 30.709 635 354 19 1 622 261 822 1.08e-88 298
MS.gene32730.t1 MTR_5g059920 33.816 553 311 14 1 547 264 767 2.93e-88 294
MS.gene32730.t1 MTR_2g015280 30.346 636 359 18 1 628 258 817 3.94e-86 291
MS.gene32730.t1 MTR_6g045087 39.109 404 227 9 222 611 39 437 3.90e-82 270
MS.gene32730.t1 MTR_8g073210 37.226 411 236 9 1 401 248 646 2.04e-76 259
MS.gene32730.t1 MTR_3g115910 36.272 397 229 9 1 389 247 627 1.18e-75 257
MS.gene32730.t1 MTR_3g115910 36.272 397 229 9 1 389 292 672 2.70e-75 257
MS.gene32730.t1 MTR_2g088450 37.037 405 237 9 1 399 154 546 2.51e-73 248
MS.gene32730.t1 MTR_0018s0230 29.381 582 333 16 1 573 169 681 7.40e-73 250
MS.gene32730.t1 MTR_2g088450 37.037 405 237 9 1 399 252 644 2.65e-72 248
MS.gene32730.t1 MTR_8g073210 36.341 399 232 9 1 389 248 634 7.10e-71 244
MS.gene32730.t1 MTR_2g088450 36.409 401 237 9 1 395 154 542 9.68e-70 238
MS.gene32730.t1 MTR_5g060330 33.974 312 166 9 1 307 170 446 1.04e-42 165
MS.gene32730.t1 MTR_5g060330 35.333 150 82 6 500 637 466 612 3.69e-15 79.7
MS.gene32730.t1 MTR_8g073210 31.475 305 188 8 1 295 248 541 4.26e-35 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32730.t1 AT2G29120 49.644 703 296 17 1 688 253 912 0.0 654
MS.gene32730.t1 AT2G29110 50.723 692 297 17 1 688 245 896 0.0 635
MS.gene32730.t1 AT2G29110 50.723 692 297 17 1 688 244 895 0.0 635
MS.gene32730.t1 AT2G29100 48.000 700 321 15 1 693 243 906 0.0 620
MS.gene32730.t1 AT2G29100 48.000 700 321 15 1 693 241 904 0.0 620
MS.gene32730.t1 AT5G27100 46.687 664 304 14 1 652 246 871 0.0 587
MS.gene32730.t1 AT2G24720 50.252 595 271 10 1 589 246 821 0.0 585
MS.gene32730.t1 AT2G24710 47.022 638 309 10 1 627 245 864 0.0 574
MS.gene32730.t1 AT2G24710 47.022 638 309 10 1 627 269 888 0.0 573
MS.gene32730.t1 AT5G11210 44.888 626 322 9 1 622 251 857 0.0 553
MS.gene32730.t1 AT5G11210 44.888 626 322 9 1 622 169 775 0.0 553
MS.gene32730.t1 AT5G11210 44.586 628 321 9 1 622 193 799 0.0 552
MS.gene32730.t1 AT5G11180 44.255 644 335 11 1 635 250 878 0.0 548
MS.gene32730.t1 AT4G31710 45.625 640 300 13 1 628 246 849 1.94e-174 523
MS.gene32730.t1 AT2G24710 48.250 543 259 8 1 539 269 793 3.26e-173 517
MS.gene32730.t1 AT4G31710 44.757 639 298 13 1 627 269 864 3.22e-169 510
MS.gene32730.t1 AT5G11210 44.015 543 279 7 1 538 169 691 4.51e-160 480
MS.gene32730.t1 AT5G11210 44.015 543 279 7 1 538 169 691 4.60e-160 480
MS.gene32730.t1 AT5G11210 44.015 543 279 7 1 538 169 691 4.60e-160 480
MS.gene32730.t1 AT5G11210 44.362 541 280 7 1 538 177 699 5.09e-160 480
MS.gene32730.t1 AT5G11210 44.362 541 280 7 1 538 251 773 2.68e-159 481
MS.gene32730.t1 AT5G11210 44.362 541 280 7 1 538 251 773 2.68e-159 481
MS.gene32730.t1 AT5G11210 44.362 541 280 7 1 538 251 773 3.77e-159 480
MS.gene32730.t1 AT5G11180 39.658 643 305 15 1 635 250 817 1.20e-144 446
MS.gene32730.t1 AT1G42540 37.482 691 380 16 1 665 245 909 6.63e-144 445
MS.gene32730.t1 AT1G42540 37.482 691 380 16 1 665 245 909 6.63e-144 445
MS.gene32730.t1 AT1G42540 37.482 691 380 16 1 665 245 909 6.63e-144 445
MS.gene32730.t1 AT4G35290 36.578 678 388 19 1 659 247 901 5.37e-132 413
MS.gene32730.t1 AT4G35290 36.578 678 388 19 1 659 247 901 5.37e-132 413
MS.gene32730.t1 AT4G35290 36.578 678 388 19 1 659 247 901 5.37e-132 413
MS.gene32730.t1 AT4G35290 36.578 678 388 19 1 659 247 901 5.37e-132 413
MS.gene32730.t1 AT4G35290 36.578 678 388 19 1 659 247 901 5.37e-132 413
MS.gene32730.t1 AT2G17260 38.325 621 351 18 1 611 251 849 1.31e-128 405
MS.gene32730.t1 AT3G51480 34.015 685 410 16 1 675 154 806 2.50e-124 390
MS.gene32730.t1 AT1G05200 34.762 630 387 11 1 623 275 887 9.64e-124 393
MS.gene32730.t1 AT1G05200 34.762 630 387 11 1 623 275 887 9.64e-124 393
MS.gene32730.t1 AT1G05200 34.762 630 387 11 1 623 275 887 9.64e-124 393
MS.gene32730.t1 AT3G51480 34.015 685 410 16 1 675 244 896 1.96e-123 390
MS.gene32730.t1 AT3G51480 34.015 685 410 16 1 675 244 896 1.96e-123 390
MS.gene32730.t1 AT2G32390 36.133 631 383 12 1 623 160 778 1.02e-121 385
MS.gene32730.t1 AT2G32390 36.133 631 383 12 1 623 204 822 1.79e-121 385
MS.gene32730.t1 AT2G32390 36.292 631 382 12 1 623 262 880 2.55e-120 384
MS.gene32730.t1 AT2G32400 33.381 704 419 18 1 683 245 919 1.13e-112 363
MS.gene32730.t1 AT3G07520 35.202 642 332 17 1 627 260 832 1.15e-110 356
MS.gene32730.t1 AT5G11210 49.853 341 165 5 284 622 13 349 4.43e-109 338
MS.gene32730.t1 AT5G48410 34.116 639 336 18 1 627 255 820 3.34e-106 344
MS.gene32730.t1 AT4G35290 37.044 548 312 15 1 538 247 771 9.78e-106 340
MS.gene32730.t1 AT5G48400 33.490 639 345 20 1 627 253 823 4.46e-105 341
MS.gene32730.t1 AT1G05200 34.545 550 336 11 1 543 275 807 1.60e-102 333
MS.gene32730.t1 AT1G05200 34.545 550 336 11 1 543 275 807 1.60e-102 333
MS.gene32730.t1 AT3G51480 34.489 548 322 13 1 538 244 764 3.75e-98 320
MS.gene32730.t1 AT5G48410 34.909 550 276 17 1 538 255 734 5.39e-93 306
MS.gene32730.t1 AT5G48400 34.000 550 286 18 1 538 253 737 3.21e-91 301
MS.gene32730.t1 AT5G48410 32.394 639 320 19 1 627 255 793 2.15e-90 301
MS.gene32730.t1 AT5G48410 32.238 639 317 19 1 627 255 789 1.60e-88 296
MS.gene32730.t1 AT3G04110 29.440 625 346 19 1 611 244 787 5.22e-79 270
MS.gene32730.t1 AT2G32390 36.145 415 249 10 1 408 262 667 1.50e-77 263
MS.gene32730.t1 AT1G42540 36.715 414 227 11 1 399 245 638 1.86e-77 262
MS.gene32730.t1 AT2G32390 34.286 420 244 10 1 405 262 664 2.03e-74 255
MS.gene32730.t1 AT2G32390 34.286 420 244 10 1 405 262 664 2.03e-74 255
MS.gene32730.t1 AT1G05200 33.659 410 252 9 1 404 275 670 5.01e-71 245
MS.gene32730.t1 AT2G32390 34.761 397 243 10 1 390 262 649 2.02e-69 240
MS.gene32730.t1 AT1G05200 33.081 396 245 9 1 390 275 656 5.51e-66 231
MS.gene32730.t1 AT3G04110 28.598 542 302 16 1 538 244 704 5.96e-65 230
MS.gene32730.t1 AT3G04110 26.880 625 334 20 1 611 244 759 6.23e-62 222

Find 155 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATCACTCAACTTTATTA+TGG 0.166418 3.3:-86842088 MS.gene32730:CDS
TTTGATTCAGTTTGACTAAA+TGG 0.183703 3.3:+86839543 None:intergenic
TGAAGGGTTTGTTAGAAATT+TGG 0.183990 3.3:-86842437 MS.gene32730:CDS
TTGTTAAACACTAGATTTAA+TGG 0.210689 3.3:-86842568 MS.gene32730:CDS
GGAATTGGAGCCACTAAATT+TGG 0.217370 3.3:-86842682 MS.gene32730:CDS
GTCTTGGCCTTGAAAGCTTC+TGG 0.218697 3.3:-86839842 MS.gene32730:CDS
TGCACAAAATGGTGAGAAAT+TGG 0.227683 3.3:-86842599 MS.gene32730:CDS
CCTTATCATGATGATGATTT+AGG 0.232260 3.3:-86839606 MS.gene32730:CDS
CCCTATGTTAAGCAATTTCT+TGG 0.234449 3.3:-86840464 MS.gene32730:CDS
CAAGATTTGTGGTGGTTATA+TGG 0.248414 3.3:-86840763 MS.gene32730:CDS
AGCCACTCACATGGGATCTT+TGG 0.254131 3.3:-86841494 MS.gene32730:CDS
CAAAGATTAGGAGTGCTAAT+AGG 0.255670 3.3:+86839790 None:intergenic
TATTCATTGGATTTGTTGTA+TGG 0.275412 3.3:-86841449 MS.gene32730:CDS
TTGACTAAAATCTCCATAAA+AGG 0.279640 3.3:+86839515 None:intergenic
GCTTGTTCATTTGTATTCAT+TGG 0.284251 3.3:-86841462 MS.gene32730:CDS
TGCCTCCTTGAGTCACATTT+AGG 0.292586 3.3:+86839943 None:intergenic
GGATGGGAAATTCCTACTAT+TGG 0.294555 3.3:-86842313 MS.gene32730:CDS
AGATTTAAAACGGGTGGTTT+TGG 0.295033 3.3:-86840398 MS.gene32730:CDS
GCTACCAGAGGTGAACCTTT+TGG 0.295492 3.3:+86839984 None:intergenic
TAAGATGAATGTTGGCTATC+TGG 0.299440 3.3:-86840630 MS.gene32730:CDS
AAGAAAACAGAAAATATAAA+TGG 0.304802 3.3:-86839642 MS.gene32730:CDS
TTGTTCACAGGGGAAACTTC+TGG 0.310514 3.3:+86839459 None:intergenic
ATCAGATTGGCACAAGCTTA+TGG 0.317813 3.3:-86841380 MS.gene32730:CDS
CGTCTCCATATTGAATTACT+TGG 0.322004 3.3:+86839720 None:intergenic
AAAAGCTCATTACATTCTTC+AGG 0.333198 3.3:+86840536 None:intergenic
ATTAGAGGAAATTTCTGTAT+TGG 0.334978 3.3:+86839869 None:intergenic
TGATCACTCAACTTTATTAT+GGG 0.343974 3.3:-86842087 MS.gene32730:CDS
TTGAGTGATCAAATCATTGT+AGG 0.359138 3.3:+86842099 None:intergenic
AAGGCCAAGACTATTATTAG+AGG 0.373292 3.3:+86839854 None:intergenic
TGGAAGGAGTATTGGGTGTT+AGG 0.378795 3.3:-86842864 MS.gene32730:CDS
CGTTGTTTATTCTATGTTCA+AGG 0.381373 3.3:+86841424 None:intergenic
GCCACTCACATGGGATCTTT+GGG 0.382157 3.3:-86841493 MS.gene32730:CDS
TCTTGGCCTTGAAAGCTTCT+GGG 0.382812 3.3:-86839841 MS.gene32730:CDS
AAGTAATGAGCTGAGATTCT+TGG 0.388859 3.3:+86840567 None:intergenic
CCATGTGAGTGGCTTCAAAA+AGG 0.389028 3.3:+86841503 None:intergenic
CTCTGGTAGCAGACATTTCA+AGG 0.390581 3.3:-86839971 MS.gene32730:CDS
AAAGATTAGGAGTGCTAATA+GGG 0.396128 3.3:+86839791 None:intergenic
ACTAGATTTAATGGTCTTAG+TGG 0.396942 3.3:-86842559 MS.gene32730:CDS
TACATCAGCACAAACACATT+TGG 0.398481 3.3:-86839755 MS.gene32730:CDS
ATAATCCAAGTAATTCAATA+TGG 0.398624 3.3:-86839725 MS.gene32730:CDS
CTCATTGTAACCAAATTTAG+TGG 0.401817 3.3:+86842672 None:intergenic
TGATGAGTCAAGGCTATGTT+TGG 0.402077 3.3:-86842948 MS.gene32730:CDS
AAAGACCCCAGAAGCTTTCA+AGG 0.404234 3.3:+86839835 None:intergenic
TCAGAACTTTGATGCGGTGG+TGG 0.404424 3.3:-86841643 MS.gene32730:intron
GATGGGAAATTCCTACTATT+GGG 0.406766 3.3:-86842312 MS.gene32730:CDS
TGCATTAGCAATGGCTATTG+AGG 0.408558 3.3:-86842710 MS.gene32730:CDS
AGTGGTGATTTCAATGTTGT+TGG 0.410007 3.3:-86842541 MS.gene32730:CDS
GCTATTGAGGAAGTTGGAAT+TGG 0.411599 3.3:-86842697 MS.gene32730:CDS
GAATCAATGGAAGGAGTATT+GGG 0.414833 3.3:-86842871 MS.gene32730:CDS
AGCAAAGACCATAGTTGAAA+AGG 0.419810 3.3:+86841353 None:intergenic
GTAGGAATTTCCCATCCCTT+TGG 0.420382 3.3:+86842319 None:intergenic
TTGGATAGCAACCGATAGTA+TGG 0.420520 3.3:-86842929 MS.gene32730:CDS
ATTAGCACTCCTAATCTTTG+TGG 0.423040 3.3:-86839787 MS.gene32730:CDS
AAGATTTGTGGTGGTTATAT+GGG 0.425424 3.3:-86840762 MS.gene32730:CDS
TTCATAAAAGGAAGTGCAAA+TGG 0.428259 3.3:-86840515 MS.gene32730:CDS
TCATCTTATTCTTTAGAAGT+TGG 0.431478 3.3:+86840645 None:intergenic
GTTAGAAATTTGGACACAAA+AGG 0.435100 3.3:-86842427 MS.gene32730:CDS
GGAATCAATGGAAGGAGTAT+TGG 0.436571 3.3:-86842872 MS.gene32730:CDS
ACAATGCCATACACAGAATC+TGG 0.438361 3.3:-86841576 MS.gene32730:CDS
CTAAATGGACTAGGTGGATA+AGG 0.447867 3.3:+86839558 None:intergenic
GGTTGAGCCAAGATTTAAAA+CGG 0.451460 3.3:-86840408 MS.gene32730:CDS
GCAAATGGAACAAAGTCATA+TGG 0.454887 3.3:+86842148 None:intergenic
TCACCATCAGGCTTTGCAAA+TGG 0.456931 3.3:+86842133 None:intergenic
TAGATGATCTCGTGTTGCTT+GGG 0.457998 3.3:+86839422 None:intergenic
ATAGATGATCTCGTGTTGCT+TGG 0.460591 3.3:+86839421 None:intergenic
AAATTTGTGTCAACAAGTGT+TGG 0.461667 3.3:+86842769 None:intergenic
GCACAAAATGGTGAGAAATT+GGG 0.464067 3.3:-86842598 MS.gene32730:CDS
CTAGGTGGATAAGGTGTACC+TGG 0.468506 3.3:+86839567 None:intergenic
TTCTTCTTCAAAGTATGTCA+TGG 0.471946 3.3:-86840429 MS.gene32730:CDS
AAGTCATATGGTAATGCATA+AGG 0.472377 3.3:+86842160 None:intergenic
TATGAAATAATTAATGTGAT+TGG 0.474278 3.3:-86842496 MS.gene32730:CDS
CAGAACTTTGATGCGGTGGT+GGG 0.476644 3.3:-86841642 MS.gene32730:CDS
TTATAATGTGAGGGAATCAA+TGG 0.479009 3.3:-86842884 MS.gene32730:CDS
GTCACACCAGATTCTGTGTA+TGG 0.482816 3.3:+86841570 None:intergenic
CCAAGAAATTGCTTAACATA+GGG 0.484014 3.3:+86840464 None:intergenic
GAAACGTTACCACAAAGATT+AGG 0.484191 3.3:+86839778 None:intergenic
TTAATTTCACAGTTGTTCAC+AGG 0.488033 3.3:+86839447 None:intergenic
CCTAAATCATCATCATGATA+AGG 0.490096 3.3:+86839606 None:intergenic
CTTGGGGTAATCATGTGGCC+AGG 0.494176 3.3:-86842358 MS.gene32730:CDS
TGGTTAACGTCTGTAATAGC+TGG 0.497231 3.3:+86840665 None:intergenic
TCCAAGAAATTGCTTAACAT+AGG 0.508982 3.3:+86840463 None:intergenic
ATTCATTGGATTTGTTGTAT+GGG 0.509809 3.3:-86841448 MS.gene32730:CDS
ATTTCCTCTAATAATAGTCT+TGG 0.512414 3.3:-86839858 MS.gene32730:CDS
TGAACTCTATTCCAAAGGGA+TGG 0.512593 3.3:-86842330 MS.gene32730:CDS
GCAATGGCTATTGAGGAAGT+TGG 0.514964 3.3:-86842703 MS.gene32730:CDS
GTTCCCGCCATCTCACCATC+AGG 0.517404 3.3:+86842121 None:intergenic
GAACTTTGATGCGGTGGTGG+GGG 0.518222 3.3:-86841640 MS.gene32730:CDS
TGATGATTTAGGTGCGGTAG+AGG 0.522774 3.3:-86839595 MS.gene32730:CDS
GTTGAGCCAAGATTTAAAAC+GGG 0.524047 3.3:-86840407 MS.gene32730:CDS
GAGTAACTTGGCAAGATTTG+TGG 0.524831 3.3:-86840774 MS.gene32730:CDS
GGAATAGAGTTCATATCCCC+TGG 0.528712 3.3:+86842340 None:intergenic
CTCAACTTCTTCCCAATAGT+AGG 0.530247 3.3:+86842301 None:intergenic
GACGAATTGAAGTCATGGTT+AGG 0.530841 3.3:-86839701 MS.gene32730:CDS
CTACTATTGGGAAGAAGTTG+AGG 0.532909 3.3:-86842300 MS.gene32730:CDS
TAATTAATGTGATTGGTGAT+GGG 0.533125 3.3:-86842489 MS.gene32730:CDS
AATTTGTAGGCATTCCCAAA+AGG 0.535074 3.3:-86839999 MS.gene32730:intron
CTAAAGAATAAGATGAATGT+TGG 0.535880 3.3:-86840638 MS.gene32730:CDS
AGAACTTTGATGCGGTGGTG+GGG 0.537141 3.3:-86841641 MS.gene32730:CDS
GATCACTCAACTTTATTATG+GGG 0.541790 3.3:-86842086 MS.gene32730:intron
ATAATTAATGTGATTGGTGA+TGG 0.544016 3.3:-86842490 MS.gene32730:CDS
GGTGCGGTAGAGGCCTCTCC+AGG 0.545355 3.3:-86839585 MS.gene32730:CDS
TCAGTTTGACTAAATGGACT+AGG 0.546319 3.3:+86839549 None:intergenic
TTCCCTCACATTATAACTCA+AGG 0.547803 3.3:+86842891 None:intergenic
TTCCTTGAGTTATAATGTGA+GGG 0.548152 3.3:-86842893 MS.gene32730:CDS
GAACTCTATTCCAAAGGGAT+GGG 0.551466 3.3:-86842329 MS.gene32730:CDS
GGTGATTTCAATGTTGTTGG+TGG 0.552700 3.3:-86842538 MS.gene32730:CDS
GATATGAACTCTATTCCAAA+GGG 0.552824 3.3:-86842334 MS.gene32730:CDS
GAATAAATTAGCCATACTAT+CGG 0.555646 3.3:+86842918 None:intergenic
TGAGAACAATTGAGAATGCA+TGG 0.556111 3.3:-86839917 MS.gene32730:CDS
GGACCAACCTCTCATCAGAT+TGG 0.557408 3.3:-86841393 MS.gene32730:CDS
CTTGGCCTTGAAAGCTTCTG+GGG 0.560766 3.3:-86839840 MS.gene32730:CDS
GTGCCAATCTGATGAGAGGT+TGG 0.561854 3.3:+86841390 None:intergenic
ATTCCTTGAGTTATAATGTG+AGG 0.565592 3.3:-86842894 MS.gene32730:CDS
TTGGGGTAATCATGTGGCCA+GGG 0.567541 3.3:-86842357 MS.gene32730:CDS
ATTGGGAAGAAGTTGAGGAT+AGG 0.568114 3.3:-86842295 MS.gene32730:CDS
ATTCCCAAAAGGTTCACCTC+TGG 0.569784 3.3:-86839988 MS.gene32730:CDS
AAGCCTGATGGTGAGATGGC+GGG 0.571630 3.3:-86842124 MS.gene32730:CDS
CATTGATGTCTTCAAAGCTG+TGG 0.588475 3.3:-86842194 MS.gene32730:CDS
GTTCCATTTGCAAAGCCTGA+TGG 0.590563 3.3:-86842136 MS.gene32730:CDS
CATTGTGAAATCAACATAGT+CGG 0.591849 3.3:+86841593 None:intergenic
TGCTACAATTGCATTAGCAA+TGG 0.595360 3.3:-86842719 MS.gene32730:CDS
AGAGAGAGTAGTGAGTAACT+TGG 0.600457 3.3:-86840786 MS.gene32730:CDS
GTGATTTCAATGTTGTTGGT+GGG 0.601031 3.3:-86842537 MS.gene32730:CDS
ATGAATGTTGGCTATCTGGA+AGG 0.602535 3.3:-86840626 MS.gene32730:CDS
ACTAATTCAACACTAGCCAC+TGG 0.602738 3.3:-86842223 MS.gene32730:CDS
ATGGAGACGAATTGAAGTCA+TGG 0.605611 3.3:-86839706 MS.gene32730:CDS
TAATTTCACAGTTGTTCACA+GGG 0.607703 3.3:+86839448 None:intergenic
GGATCAGAACTTTGATGCGG+TGG 0.608343 3.3:-86841646 MS.gene32730:intron
AATTAATGTGATTGGTGATG+GGG 0.612269 3.3:-86842488 MS.gene32730:CDS
GGATATGAACTCTATTCCAA+AGG 0.613437 3.3:-86842335 MS.gene32730:CDS
CAAATTGGGATGATGAGTCA+AGG 0.617011 3.3:-86842958 None:intergenic
GCAATCCTAAATGTGACTCA+AGG 0.617571 3.3:-86839948 MS.gene32730:CDS
TGCAAAGCCTGATGGTGAGA+TGG 0.620042 3.3:-86842128 MS.gene32730:CDS
TGGGGTAATCATGTGGCCAG+GGG 0.623869 3.3:-86842356 MS.gene32730:CDS
TTTCTAACAAACCCTTCATG+TGG 0.624401 3.3:+86842442 None:intergenic
CACAGAATCTGGTGTGACAA+TGG 0.627498 3.3:-86841565 MS.gene32730:CDS
AATGTGAGGGAATCAATGGA+AGG 0.632436 3.3:-86842880 MS.gene32730:CDS
TGGATAAGGTGTACCTGGAG+AGG 0.633371 3.3:+86839572 None:intergenic
ATGATCTTGGGGTAATCATG+TGG 0.635275 3.3:-86842363 MS.gene32730:CDS
GAGCCAAGATTTAAAACGGG+TGG 0.636705 3.3:-86840404 MS.gene32730:CDS
AGAATAAACAACGATTTCAG+AGG 0.640862 3.3:-86841414 MS.gene32730:CDS
TGATGTCTTCAAAGCTGTGG+TGG 0.644862 3.3:-86842191 MS.gene32730:CDS
GTTTGACTAAATGGACTAGG+TGG 0.645627 3.3:+86839552 None:intergenic
ACCCAAAGATCCCATGTGAG+TGG 0.646306 3.3:+86841492 None:intergenic
TCATGATGATGATTTAGGTG+CGG 0.658204 3.3:-86839601 MS.gene32730:CDS
TAACTTGGCAAGATTTGTGG+TGG 0.662110 3.3:-86840771 MS.gene32730:CDS
ATAAAAGGAAGTGCAAATGG+TGG 0.667432 3.3:-86840512 MS.gene32730:CDS
ATCCTAAATGTGACTCAAGG+AGG 0.671234 3.3:-86839945 MS.gene32730:CDS
TTTGGATCAGAACTTTGATG+CGG 0.679944 3.3:-86841649 MS.gene32730:intron
TCTGGTAGCAGACATTTCAA+GGG 0.681450 3.3:-86839970 MS.gene32730:CDS
GACATCAATGCAGAAGCCAG+TGG 0.681497 3.3:+86842207 None:intergenic
AAAGCCTGATGGTGAGATGG+CGG 0.684105 3.3:-86842125 MS.gene32730:CDS
CTTGAAATGTCTGCTACCAG+AGG 0.702261 3.3:+86839972 None:intergenic
GCTTGTGCCAATCTGATGAG+AGG 0.718185 3.3:+86841386 None:intergenic
AATTTCACAGTTGTTCACAG+GGG 0.729956 3.3:+86839449 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGACATTTTATTATTTAA+TGG + chr3.3:86840518-86840537 None:intergenic 10.0%
!! AAGAAAACAGAAAATATAAA+TGG - chr3.3:86842726-86842745 MS.gene32730:CDS 15.0%
!! AATTATGATGACATTATATT+AGG + chr3.3:86842034-86842053 None:intergenic 15.0%
!! ATTATGATGACATTATATTA+GGG + chr3.3:86842033-86842052 None:intergenic 15.0%
!! TATGAAATAATTAATGTGAT+TGG - chr3.3:86839872-86839891 MS.gene32730:CDS 15.0%
!!! TTAAAAGTATACTAAGAATT+TGG + chr3.3:86840380-86840399 None:intergenic 15.0%
!!! TTGTTTATTTATTTCTCATT+TGG - chr3.3:86842234-86842253 MS.gene32730:CDS 15.0%
!! AAGTTATTCTATCACAAATT+AGG - chr3.3:86841256-86841275 MS.gene32730:intron 20.0%
!! ATAATCCAAGTAATTCAATA+TGG - chr3.3:86842643-86842662 MS.gene32730:CDS 20.0%
!! TATAGAAATCTAGAGAAAAT+AGG - chr3.3:86841430-86841449 MS.gene32730:CDS 20.0%
!! TATGAAATATGTGAACTTTA+TGG - chr3.3:86840402-86840421 MS.gene32730:CDS 20.0%
!! TGAAATAACCTAAATATCAT+AGG - chr3.3:86840677-86840696 MS.gene32730:CDS 20.0%
!! TTGTTAAACACTAGATTTAA+TGG - chr3.3:86839800-86839819 MS.gene32730:CDS 20.0%
!!! ATACCAAAATTTTCAAAAGT+AGG + chr3.3:86839749-86839768 None:intergenic 20.0%
!!! ATTTGAACATTTTTGGAATA+TGG - chr3.3:86839618-86839637 MS.gene32730:CDS 20.0%
!!! TTTGAACATTTTTGGAATAT+GGG - chr3.3:86839619-86839638 MS.gene32730:CDS 20.0%
! AACTTATACATGAACACTTA+TGG + chr3.3:86841241-86841260 None:intergenic 25.0%
! AATCTAGAGAAAATAGGTTA+TGG - chr3.3:86841436-86841455 MS.gene32730:CDS 25.0%
! AGTATCACAAGTATTTACAT+CGG - chr3.3:86841493-86841512 MS.gene32730:CDS 25.0%
! AGTTTATCAGAAACCATATT+TGG + chr3.3:86841309-86841328 None:intergenic 25.0%
! ATAATTAATGTGATTGGTGA+TGG - chr3.3:86839878-86839897 MS.gene32730:CDS 25.0%
! ATGCATGAAATTGTAAGAAA+TGG - chr3.3:86840453-86840472 MS.gene32730:CDS 25.0%
! ATTAGAGGAAATTTCTGTAT+TGG + chr3.3:86842502-86842521 None:intergenic 25.0%
! ATTTCCTCTAATAATAGTCT+TGG - chr3.3:86842510-86842529 MS.gene32730:CDS 25.0%
! CATTCACATATATAAGATCA+GGG + chr3.3:86840332-86840351 None:intergenic 25.0%
! CTAAAGAATAAGATGAATGT+TGG - chr3.3:86841730-86841749 MS.gene32730:intron 25.0%
! GAACTTTATGGTTTCTTTAT+TGG - chr3.3:86840414-86840433 MS.gene32730:CDS 25.0%
! GAATAAATTAGCCATACTAT+CGG + chr3.3:86839453-86839472 None:intergenic 25.0%
! TCACAAATTAGGAAAGATTT+AGG - chr3.3:86841267-86841286 MS.gene32730:intron 25.0%
! TCATTCACATATATAAGATC+AGG + chr3.3:86840333-86840352 None:intergenic 25.0%
! TGATCACTCAACTTTATTAT+GGG - chr3.3:86840281-86840300 MS.gene32730:intron 25.0%
! TTGACTAAAATCTCCATAAA+AGG + chr3.3:86842856-86842875 None:intergenic 25.0%
! TTGATCACTCAACTTTATTA+TGG - chr3.3:86840280-86840299 MS.gene32730:intron 25.0%
! TTTGATTCAGTTTGACTAAA+TGG + chr3.3:86842828-86842847 None:intergenic 25.0%
!! AGTATGTTAGGTTTGAATTT+TGG + chr3.3:86842076-86842095 None:intergenic 25.0%
!! CTACTTCTAAAAATGATCTT+GGG - chr3.3:86839993-86840012 MS.gene32730:CDS 25.0%
!! GAATCAAATGTTTCCTTTTA+TGG - chr3.3:86842840-86842859 MS.gene32730:CDS 25.0%
!! GACACAAATTTGAACATTTT+TGG - chr3.3:86839611-86839630 MS.gene32730:CDS 25.0%
!! TAATTAATGTGATTGGTGAT+GGG - chr3.3:86839879-86839898 MS.gene32730:CDS 25.0%
!! TACTTCTAAAAATGATCTTG+GGG - chr3.3:86839994-86840013 MS.gene32730:intron 25.0%
!! TCATCTTATTCTTTAGAAGT+TGG + chr3.3:86841726-86841745 None:intergenic 25.0%
!! TCTACTTCTAAAAATGATCT+TGG - chr3.3:86839992-86840011 MS.gene32730:CDS 25.0%
!! TGTAATGAGCTTTTCATAAA+AGG - chr3.3:86841841-86841860 MS.gene32730:intron 25.0%
!! TTTTAAACTCTCAAAGAGTA+GGG + chr3.3:86840489-86840508 None:intergenic 25.0%
!! TTTTAGTCAAAGCATAGAAA+TGG - chr3.3:86842866-86842885 MS.gene32730:CDS 25.0%
!! TTTTTAAACTCTCAAAGAGT+AGG + chr3.3:86840490-86840509 None:intergenic 25.0%
!!! AATTTTGGTATTGCACAAAA+TGG - chr3.3:86839758-86839777 MS.gene32730:CDS 25.0%
!!! ATGCCTACTTTTGAAAATTT+TGG - chr3.3:86839743-86839762 MS.gene32730:CDS 25.0%
!!! ATTCATTGGATTTGTTGTAT+GGG - chr3.3:86840920-86840939 MS.gene32730:intron 25.0%
!!! TATTCATTGGATTTGTTGTA+TGG - chr3.3:86840919-86840938 MS.gene32730:intron 25.0%
!!! TTTTGTTTATGGAATCTTGA+AGG - chr3.3:86841768-86841787 MS.gene32730:intron 25.0%
AAAAGCTCATTACATTCTTC+AGG + chr3.3:86841835-86841854 None:intergenic 30.0%
AAATTTGTGTCAACAAGTGT+TGG + chr3.3:86839602-86839621 None:intergenic 30.0%
AAGATTTGTGGTGGTTATAT+GGG - chr3.3:86841606-86841625 MS.gene32730:CDS 30.0%
ACAACAGAAAGAAAAATGCA+TGG - chr3.3:86840841-86840860 MS.gene32730:intron 30.0%
ACTCATTGAATCAGTATGTT+AGG + chr3.3:86842088-86842107 None:intergenic 30.0%
AGAATAAACAACGATTTCAG+AGG - chr3.3:86840954-86840973 MS.gene32730:intron 30.0%
ATAGGATTACCAGTGAAAAA+TGG - chr3.3:86840091-86840110 MS.gene32730:intron 30.0%
ATTCCTTGAGTTATAATGTG+AGG - chr3.3:86839474-86839493 MS.gene32730:CDS 30.0%
CAACAGAAAGAAAAATGCAT+GGG - chr3.3:86840842-86840861 MS.gene32730:intron 30.0%
CATTGTGAAATCAACATAGT+CGG + chr3.3:86840778-86840797 None:intergenic 30.0%
CCAAGAAATTGCTTAACATA+GGG + chr3.3:86841907-86841926 None:intergenic 30.0%
CCTAAATCATCATCATGATA+AGG + chr3.3:86842765-86842784 None:intergenic 30.0%
CGTTGTTTATTCTATGTTCA+AGG + chr3.3:86840947-86840966 None:intergenic 30.0%
CTCATTGTAACCAAATTTAG+TGG + chr3.3:86839699-86839718 None:intergenic 30.0%
CTTCTAGGTTATAGTTCAAT+TGG + chr3.3:86842276-86842295 None:intergenic 30.0%
GATATGAACTCTATTCCAAA+GGG - chr3.3:86840034-86840053 MS.gene32730:intron 30.0%
GATCACTCAACTTTATTATG+GGG - chr3.3:86840282-86840301 MS.gene32730:intron 30.0%
GCTTGTTCATTTGTATTCAT+TGG - chr3.3:86840906-86840925 MS.gene32730:intron 30.0%
GTTAGAAATTTGGACACAAA+AGG - chr3.3:86839941-86839960 MS.gene32730:CDS 30.0%
TAATTTCACAGTTGTTCACA+GGG + chr3.3:86842923-86842942 None:intergenic 30.0%
TCCAAGAAATTGCTTAACAT+AGG + chr3.3:86841908-86841927 None:intergenic 30.0%
TCCTAGAACAAAAAAACTAG+AGG - chr3.3:86839535-86839554 MS.gene32730:CDS 30.0%
TTAATTTCACAGTTGTTCAC+AGG + chr3.3:86842924-86842943 None:intergenic 30.0%
TTACTGACAAAGTAGTCATT+AGG + chr3.3:86840618-86840637 None:intergenic 30.0%
TTATAATGTGAGGGAATCAA+TGG - chr3.3:86839484-86839503 MS.gene32730:CDS 30.0%
TTCCTTGAGTTATAATGTGA+GGG - chr3.3:86839475-86839494 MS.gene32730:CDS 30.0%
TTCTTCTTCAAAGTATGTCA+TGG - chr3.3:86841939-86841958 MS.gene32730:intron 30.0%
TTGAGTGATCAAATCATTGT+AGG + chr3.3:86840272-86840291 None:intergenic 30.0%
! AAGTCATATGGTAATGCATA+AGG + chr3.3:86840211-86840230 None:intergenic 30.0%
! ACTAGATTTAATGGTCTTAG+TGG - chr3.3:86839809-86839828 MS.gene32730:CDS 30.0%
! ACTGATTCAATGAGTTGAAT+TGG - chr3.3:86842093-86842112 MS.gene32730:CDS 30.0%
! AGTACTAACTTGACTTTTGT+GGG - chr3.3:86842342-86842361 MS.gene32730:CDS 30.0%
! ATGGTTTTCCTTTTCAACTA+TGG - chr3.3:86841007-86841026 MS.gene32730:intron 30.0%
! CCTTATCATGATGATGATTT+AGG - chr3.3:86842762-86842781 MS.gene32730:CDS 30.0%
! CTAGTTAGCCTATGATATTT+AGG + chr3.3:86840688-86840707 None:intergenic 30.0%
! TGAATTGGTTCAACTATGAT+TGG - chr3.3:86842148-86842167 MS.gene32730:CDS 30.0%
! TTCAAGTAATCCGCAATTTT+TGG + chr3.3:86841194-86841213 None:intergenic 30.0%
! TTCATAAAAGGAAGTGCAAA+TGG - chr3.3:86841853-86841872 MS.gene32730:intron 30.0%
!! AAAGATTAGGAGTGCTAATA+GGG + chr3.3:86842580-86842599 None:intergenic 30.0%
!! AACCATCACATTTTAATCAC+AGG - chr3.3:86841559-86841578 MS.gene32730:CDS 30.0%
!! AATTAATGTGATTGGTGATG+GGG - chr3.3:86839880-86839899 MS.gene32730:CDS 30.0%
!! GCTATTTTCAAAAGCTATCA+TGG - chr3.3:86841401-86841420 MS.gene32730:CDS 30.0%
!! TAGTTATCTCCATTTTTCAC+TGG + chr3.3:86840103-86840122 None:intergenic 30.0%
!! TGAAGGGTTTGTTAGAAATT+TGG - chr3.3:86839931-86839950 MS.gene32730:CDS 30.0%
!!! GGTTTTTGTGGTTCTAATTT+TGG - chr3.3:86841627-86841646 MS.gene32730:CDS 30.0%
!!! TAGAGGATTTTAGAGTTAGA+TGG - chr3.3:86839552-86839571 MS.gene32730:CDS 30.0%
!!! TCCTCTAGTTTTTTTGTTCT+AGG + chr3.3:86839539-86839558 None:intergenic 30.0%
AAGGCCAAGACTATTATTAG+AGG + chr3.3:86842517-86842536 None:intergenic 35.0%
AAGTAATGAGCTGAGATTCT+TGG + chr3.3:86841804-86841823 None:intergenic 35.0%
AAGTTTATCCCACTACTAAG+TGG + chr3.3:86841363-86841382 None:intergenic 35.0%
AATTTCACAGTTGTTCACAG+GGG + chr3.3:86842922-86842941 None:intergenic 35.0%
AATTTGTAGGCATTCCCAAA+AGG - chr3.3:86842369-86842388 MS.gene32730:CDS 35.0%
AGCAAAGACCATAGTTGAAA+AGG + chr3.3:86841018-86841037 None:intergenic 35.0%
AGTGGTGATTTCAATGTTGT+TGG - chr3.3:86839827-86839846 MS.gene32730:CDS 35.0%
AGTTTATCCCACTACTAAGT+GGG + chr3.3:86841362-86841381 None:intergenic 35.0%
ATTAGCACTCCTAATCTTTG+TGG - chr3.3:86842581-86842600 MS.gene32730:CDS 35.0%
CAACCAGACTGCAATTAATT+GGG + chr3.3:86842125-86842144 None:intergenic 35.0%
CAAGATTTGTGGTGGTTATA+TGG - chr3.3:86841605-86841624 MS.gene32730:CDS 35.0%
CAATCTCGTTCCAAAAATTG+CGG - chr3.3:86841181-86841200 MS.gene32730:intron 35.0%
CCCTATGTTAAGCAATTTCT+TGG - chr3.3:86841904-86841923 MS.gene32730:intron 35.0%
CGTCTCCATATTGAATTACT+TGG + chr3.3:86842651-86842670 None:intergenic 35.0%
CTCCTGTGATTAAAATGTGA+TGG + chr3.3:86841564-86841583 None:intergenic 35.0%
CTGAATTGTTCGATCTTGAT+CGG + chr3.3:86841100-86841119 None:intergenic 35.0%
GAAACGTTACCACAAAGATT+AGG + chr3.3:86842593-86842612 None:intergenic 35.0%
GATGGGAAATTCCTACTATT+GGG - chr3.3:86840056-86840075 MS.gene32730:intron 35.0%
GCAAATGGAACAAAGTCATA+TGG + chr3.3:86840223-86840242 None:intergenic 35.0%
GCACAAAATGGTGAGAAATT+GGG - chr3.3:86839770-86839789 MS.gene32730:CDS 35.0%
GGATATGAACTCTATTCCAA+AGG - chr3.3:86840033-86840052 MS.gene32730:intron 35.0%
GGTTGAGCCAAGATTTAAAA+CGG - chr3.3:86841960-86841979 MS.gene32730:intron 35.0%
GTGATTTCAATGTTGTTGGT+GGG - chr3.3:86839831-86839850 MS.gene32730:CDS 35.0%
TACATCAGCACAAACACATT+TGG - chr3.3:86842613-86842632 MS.gene32730:CDS 35.0%
TCAACCAGACTGCAATTAAT+TGG + chr3.3:86842126-86842145 None:intergenic 35.0%
TCAATTCAAATACGCTCTCA+CGG - chr3.3:86841534-86841553 MS.gene32730:CDS 35.0%
TGAGAACAATTGAGAATGCA+TGG - chr3.3:86842451-86842470 MS.gene32730:CDS 35.0%
TGCACAAAATGGTGAGAAAT+TGG - chr3.3:86839769-86839788 MS.gene32730:CDS 35.0%
TGCTACAATTGCATTAGCAA+TGG - chr3.3:86839649-86839668 MS.gene32730:CDS 35.0%
TTAGGAGACCTAAAATCCAT+AGG + chr3.3:86840600-86840619 None:intergenic 35.0%
TTCCCTCACATTATAACTCA+AGG + chr3.3:86839480-86839499 None:intergenic 35.0%
TTTCTAACAAACCCTTCATG+TGG + chr3.3:86839929-86839948 None:intergenic 35.0%
TTTGGATCAGAACTTTGATG+CGG - chr3.3:86840719-86840738 MS.gene32730:CDS 35.0%
! ATAAAAGGAAGTGCAAATGG+TGG - chr3.3:86841856-86841875 MS.gene32730:intron 35.0%
! GAGTACTAACTTGACTTTTG+TGG - chr3.3:86842341-86842360 MS.gene32730:CDS 35.0%
! GTACTAACTTGACTTTTGTG+GGG - chr3.3:86842343-86842362 MS.gene32730:CDS 35.0%
! GTTGAGCCAAGATTTAAAAC+GGG - chr3.3:86841961-86841980 MS.gene32730:intron 35.0%
! TAACTAGTCCATGGTGATTT+TGG - chr3.3:86840701-86840720 MS.gene32730:CDS 35.0%
! TAAGATGAATGTTGGCTATC+TGG - chr3.3:86841738-86841757 MS.gene32730:intron 35.0%
! TACTAACTTGACTTTTGTGG+GGG - chr3.3:86842344-86842363 MS.gene32730:CDS 35.0%
! TCAGTTTGACTAAATGGACT+AGG + chr3.3:86842822-86842841 None:intergenic 35.0%
! TCATGATGATGATTTAGGTG+CGG - chr3.3:86842767-86842786 MS.gene32730:CDS 35.0%
! TGATGAGTTGGATCCAAATA+TGG - chr3.3:86841293-86841312 MS.gene32730:intron 35.0%
!! AAACCACCCGTTTTAAATCT+TGG + chr3.3:86841970-86841989 None:intergenic 35.0%
!! AGATTTAAAACGGGTGGTTT+TGG - chr3.3:86841970-86841989 MS.gene32730:intron 35.0%
!! AGATTTAGGCATTGATGAGT+TGG - chr3.3:86841281-86841300 MS.gene32730:intron 35.0%
!! CAAAGATTAGGAGTGCTAAT+AGG + chr3.3:86842581-86842600 None:intergenic 35.0%
!! CTGATTTGCCTATGGATTTT+AGG - chr3.3:86840589-86840608 MS.gene32730:CDS 35.0%
!! CTGGAAGGTTCTTTTGTTTA+TGG - chr3.3:86841757-86841776 MS.gene32730:intron 35.0%
!! GAATCAATGGAAGGAGTATT+GGG - chr3.3:86839497-86839516 MS.gene32730:CDS 35.0%
AAATCAGTTGCCACTCACAT+CGG + chr3.3:86840576-86840595 None:intergenic 40.0%
AATGTGAGGGAATCAATGGA+AGG - chr3.3:86839488-86839507 MS.gene32730:CDS 40.0%
ACAATGCCATACACAGAATC+TGG - chr3.3:86840792-86840811 MS.gene32730:intron 40.0%
ACTAATTCAACACTAGCCAC+TGG - chr3.3:86840145-86840164 MS.gene32730:intron 40.0%
AGAGAGAGTAGTGAGTAACT+TGG - chr3.3:86841582-86841601 MS.gene32730:CDS 40.0%
AGTTGATCCCACTTAGTAGT+GGG - chr3.3:86841352-86841371 MS.gene32730:CDS 40.0%
ATCAAACCAATGCGACTTCT+AGG + chr3.3:86842291-86842310 None:intergenic 40.0%
ATCAGATTGGCACAAGCTTA+TGG - chr3.3:86840988-86841007 MS.gene32730:intron 40.0%
ATCATAGGCTAACTAGTCCA+TGG - chr3.3:86840692-86840711 MS.gene32730:CDS 40.0%
ATCCTAAATGTGACTCAAGG+AGG - chr3.3:86842423-86842442 MS.gene32730:CDS 40.0%
ATGATCTTGGGGTAATCATG+TGG - chr3.3:86840005-86840024 MS.gene32730:intron 40.0%
ATGGAGACGAATTGAAGTCA+TGG - chr3.3:86842662-86842681 MS.gene32730:CDS 40.0%
ATTGGGAAGAAGTTGAGGAT+AGG - chr3.3:86840073-86840092 MS.gene32730:intron 40.0%
CATTGATGTCTTCAAAGCTG+TGG - chr3.3:86840174-86840193 MS.gene32730:intron 40.0%
CTACTATTGGGAAGAAGTTG+AGG - chr3.3:86840068-86840087 MS.gene32730:intron 40.0%
CTATAACCTAGAAGTCGCAT+TGG - chr3.3:86842282-86842301 MS.gene32730:CDS 40.0%
CTCAACTTCTTCCCAATAGT+AGG + chr3.3:86840070-86840089 None:intergenic 40.0%
GAACTCTATTCCAAAGGGAT+GGG - chr3.3:86840039-86840058 MS.gene32730:intron 40.0%
GACGAATTGAAGTCATGGTT+AGG - chr3.3:86842667-86842686 MS.gene32730:CDS 40.0%
GAGTAACTTGGCAAGATTTG+TGG - chr3.3:86841594-86841613 MS.gene32730:CDS 40.0%
GATCCCAATTAATTGCAGTC+TGG - chr3.3:86842119-86842138 MS.gene32730:CDS 40.0%
GCAATCCTAAATGTGACTCA+AGG - chr3.3:86842420-86842439 MS.gene32730:CDS 40.0%
GCAGTCTGGTTGAATTGAAT+TGG - chr3.3:86842133-86842152 MS.gene32730:CDS 40.0%
GCTATTGAGGAAGTTGGAAT+TGG - chr3.3:86839671-86839690 MS.gene32730:CDS 40.0%
GGAATTGGAGCCACTAAATT+TGG - chr3.3:86839686-86839705 MS.gene32730:CDS 40.0%
GGATGGGAAATTCCTACTAT+TGG - chr3.3:86840055-86840074 MS.gene32730:intron 40.0%
GGTGATTTCAATGTTGTTGG+TGG - chr3.3:86839830-86839849 MS.gene32730:CDS 40.0%
GTTCTGATCCAAAATCACCA+TGG + chr3.3:86840712-86840731 None:intergenic 40.0%
TAACTTGGCAAGATTTGTGG+TGG - chr3.3:86841597-86841616 MS.gene32730:CDS 40.0%
TCTGGTAGCAGACATTTCAA+GGG - chr3.3:86842398-86842417 MS.gene32730:CDS 40.0%
TGAACTCTATTCCAAAGGGA+TGG - chr3.3:86840038-86840057 MS.gene32730:intron 40.0%
TGATGAGTCAAGGCTATGTT+TGG - chr3.3:86839420-86839439 MS.gene32730:CDS 40.0%
TGCATTAGCAATGGCTATTG+AGG - chr3.3:86839658-86839677 MS.gene32730:CDS 40.0%
TGGTTAACGTCTGTAATAGC+TGG + chr3.3:86841706-86841725 None:intergenic 40.0%
TTGGATAGCAACCGATAGTA+TGG - chr3.3:86839439-86839458 MS.gene32730:CDS 40.0%
TTGTTAAAGACCGATGTGAG+TGG - chr3.3:86840563-86840582 MS.gene32730:CDS 40.0%
! ATAGATGATCTCGTGTTGCT+TGG + chr3.3:86842950-86842969 None:intergenic 40.0%
! ATGAATGTTGGCTATCTGGA+AGG - chr3.3:86841742-86841761 MS.gene32730:intron 40.0%
! GATTTTGGACACCACATGAA+GGG - chr3.3:86839915-86839934 MS.gene32730:CDS 40.0%
! GGAATCAATGGAAGGAGTAT+TGG - chr3.3:86839496-86839515 MS.gene32730:CDS 40.0%
! GGGAAAAAAGGGTTGGATTT+TGG - chr3.3:86839900-86839919 MS.gene32730:CDS 40.0%
! GGTGGTTATATGGGTTTTTG+TGG - chr3.3:86841615-86841634 MS.gene32730:CDS 40.0%
! TTTTGTGGGGGTGAATTTGT+AGG - chr3.3:86842356-86842375 MS.gene32730:CDS 40.0%
!! CATTGGTTTGATTCACGATC+GGG - chr3.3:86842299-86842318 MS.gene32730:CDS 40.0%
!! CTAAATGGACTAGGTGGATA+AGG + chr3.3:86842813-86842832 None:intergenic 40.0%
!! GATTGGTGATGGGGAAAAAA+GGG - chr3.3:86839889-86839908 MS.gene32730:CDS 40.0%
!! GCATTGGTTTGATTCACGAT+CGG - chr3.3:86842298-86842317 MS.gene32730:CDS 40.0%
!! GTTTGACTAAATGGACTAGG+TGG + chr3.3:86842819-86842838 None:intergenic 40.0%
!! TAGATGATCTCGTGTTGCTT+GGG + chr3.3:86842949-86842968 None:intergenic 40.0%
!! TGATTGGTGATGGGGAAAAA+AGG - chr3.3:86839888-86839907 MS.gene32730:CDS 40.0%
!!! CTTTTTGAAGCCACTCACAT+GGG - chr3.3:86840866-86840885 MS.gene32730:intron 40.0%
AAAGACCCCAGAAGCTTTCA+AGG + chr3.3:86842536-86842555 None:intergenic 45.0%
ACTAAGTGGGATCAACTGCA+TGG + chr3.3:86841349-86841368 None:intergenic 45.0%
ATTCCCAAAAGGTTCACCTC+TGG - chr3.3:86842380-86842399 MS.gene32730:CDS 45.0%
CACAGAATCTGGTGTGACAA+TGG - chr3.3:86840803-86840822 MS.gene32730:intron 45.0%
CAGTTGATCCCACTTAGTAG+TGG - chr3.3:86841351-86841370 MS.gene32730:CDS 45.0%
CCATGTGAGTGGCTTCAAAA+AGG + chr3.3:86840868-86840887 None:intergenic 45.0%
CTCTGGTAGCAGACATTTCA+AGG - chr3.3:86842397-86842416 MS.gene32730:CDS 45.0%
CTTGAAATGTCTGCTACCAG+AGG + chr3.3:86842399-86842418 None:intergenic 45.0%
GCAATGGCTATTGAGGAAGT+TGG - chr3.3:86839665-86839684 MS.gene32730:CDS 45.0%
GGAATAGAGTTCATATCCCC+TGG + chr3.3:86840031-86840050 None:intergenic 45.0%
GTAGGAATTTCCCATCCCTT+TGG + chr3.3:86840052-86840071 None:intergenic 45.0%
GTCACACCAGATTCTGTGTA+TGG + chr3.3:86840801-86840820 None:intergenic 45.0%
GTTCCATTTGCAAAGCCTGA+TGG - chr3.3:86840232-86840251 MS.gene32730:intron 45.0%
TCACCATCAGGCTTTGCAAA+TGG + chr3.3:86840238-86840257 None:intergenic 45.0%
TGATGATTTAGGTGCGGTAG+AGG - chr3.3:86842773-86842792 MS.gene32730:CDS 45.0%
TGATGTCTTCAAAGCTGTGG+TGG - chr3.3:86840177-86840196 MS.gene32730:intron 45.0%
TGCCTCCTTGAGTCACATTT+AGG + chr3.3:86842428-86842447 None:intergenic 45.0%
TTGTTCACAGGGGAAACTTC+TGG + chr3.3:86842912-86842931 None:intergenic 45.0%
! CTACCAGAGGTGAACCTTTT+GGG + chr3.3:86842386-86842405 None:intergenic 45.0%
! GAGCCAAGATTTAAAACGGG+TGG - chr3.3:86841964-86841983 MS.gene32730:intron 45.0%
! GGATTTTGGACACCACATGA+AGG - chr3.3:86839914-86839933 MS.gene32730:CDS 45.0%
! GGGGTCTTTTTCTCATAGCA+GGG - chr3.3:86842547-86842566 MS.gene32730:CDS 45.0%
! TGGGGTCTTTTTCTCATAGC+AGG - chr3.3:86842546-86842565 MS.gene32730:CDS 45.0%
!! AGTGGCAACTGATTTGCCTA+TGG - chr3.3:86840581-86840600 MS.gene32730:CDS 45.0%
!! TCTTGGCCTTGAAAGCTTCT+GGG - chr3.3:86842527-86842546 MS.gene32730:CDS 45.0%
!! TGGAAGGAGTATTGGGTGTT+AGG - chr3.3:86839504-86839523 MS.gene32730:CDS 45.0%
!!! CCTTTTTGAAGCCACTCACA+TGG - chr3.3:86840865-86840884 MS.gene32730:intron 45.0%
ACCCAAAGATCCCATGTGAG+TGG + chr3.3:86840879-86840898 None:intergenic 50.0%
AGAACTTTGATGCGGTGGTG+GGG - chr3.3:86840727-86840746 MS.gene32730:CDS 50.0%
AGCCACTCACATGGGATCTT+TGG - chr3.3:86840874-86840893 MS.gene32730:intron 50.0%
CAGAACTTTGATGCGGTGGT+GGG - chr3.3:86840726-86840745 MS.gene32730:CDS 50.0%
CTAGGTGGATAAGGTGTACC+TGG + chr3.3:86842804-86842823 None:intergenic 50.0%
GACATCAATGCAGAAGCCAG+TGG + chr3.3:86840164-86840183 None:intergenic 50.0%
GCCACTCACATGGGATCTTT+GGG - chr3.3:86840875-86840894 MS.gene32730:intron 50.0%
GCTTGTGCCAATCTGATGAG+AGG + chr3.3:86840985-86841004 None:intergenic 50.0%
GGACCAACCTCTCATCAGAT+TGG - chr3.3:86840975-86840994 MS.gene32730:intron 50.0%
GGATCAGAACTTTGATGCGG+TGG - chr3.3:86840722-86840741 MS.gene32730:CDS 50.0%
GTGCCAATCTGATGAGAGGT+TGG + chr3.3:86840981-86841000 None:intergenic 50.0%
TCAGAACTTTGATGCGGTGG+TGG - chr3.3:86840725-86840744 MS.gene32730:CDS 50.0%
TGGATAAGGTGTACCTGGAG+AGG + chr3.3:86842799-86842818 None:intergenic 50.0%
TTGGGGTAATCATGTGGCCA+GGG - chr3.3:86840011-86840030 MS.gene32730:intron 50.0%
! AAAGCCTGATGGTGAGATGG+CGG - chr3.3:86840243-86840262 MS.gene32730:intron 50.0%
! GCTACCAGAGGTGAACCTTT+TGG + chr3.3:86842387-86842406 None:intergenic 50.0%
! TGCAAAGCCTGATGGTGAGA+TGG - chr3.3:86840240-86840259 MS.gene32730:intron 50.0%
!! CTTGGCCTTGAAAGCTTCTG+GGG - chr3.3:86842528-86842547 MS.gene32730:CDS 50.0%
!! GGTGATGGGGAAAAAAGGGT+TGG - chr3.3:86839893-86839912 MS.gene32730:CDS 50.0%
!! GTCTTGGCCTTGAAAGCTTC+TGG - chr3.3:86842526-86842545 MS.gene32730:CDS 50.0%
CTTGGGGTAATCATGTGGCC+AGG - chr3.3:86840010-86840029 MS.gene32730:intron 55.0%
TGGGGTAATCATGTGGCCAG+GGG - chr3.3:86840012-86840031 MS.gene32730:intron 55.0%
! AAGCCTGATGGTGAGATGGC+GGG - chr3.3:86840244-86840263 MS.gene32730:intron 55.0%
! GAACTTTGATGCGGTGGTGG+GGG - chr3.3:86840728-86840747 MS.gene32730:CDS 55.0%
GTTCCCGCCATCTCACCATC+AGG + chr3.3:86840250-86840269 None:intergenic 60.0%
GGTGCGGTAGAGGCCTCTCC+AGG - chr3.3:86842783-86842802 MS.gene32730:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 86839419 86842971 86839419 ID=MS.gene32730
chr3.3 mRNA 86839419 86842971 86839419 ID=MS.gene32730.t1;Parent=MS.gene32730
chr3.3 exon 86842087 86842971 86842087 ID=MS.gene32730.t1.exon1;Parent=MS.gene32730.t1
chr3.3 CDS 86842087 86842971 86842087 ID=cds.MS.gene32730.t1;Parent=MS.gene32730.t1
chr3.3 exon 86841349 86841661 86841349 ID=MS.gene32730.t1.exon2;Parent=MS.gene32730.t1
chr3.3 CDS 86841349 86841661 86841349 ID=cds.MS.gene32730.t1;Parent=MS.gene32730.t1
chr3.3 exon 86840394 86840809 86840394 ID=MS.gene32730.t1.exon3;Parent=MS.gene32730.t1
chr3.3 CDS 86840394 86840809 86840394 ID=cds.MS.gene32730.t1;Parent=MS.gene32730.t1
chr3.3 exon 86839419 86840012 86839419 ID=MS.gene32730.t1.exon4;Parent=MS.gene32730.t1
chr3.3 CDS 86839419 86840012 86839419 ID=cds.MS.gene32730.t1;Parent=MS.gene32730.t1
Gene Sequence

>MS.gene32730

ATGATGAGTCAAGGCTATGTTTGGATAGCAACCGATAGTATGGCTAATTTATTCAATTCCTTGAGTTATAATGTGAGGGAATCAATGGAAGGAGTATTGGGTGTTAGGACTTATATTCCTAGAACAAAAAAACTAGAGGATTTTAGAGTTAGATGGAAAAGAAAATTTTTAAGTGATAATCCAACACTTGTTGACACAAATTTGAACATTTTTGGAATATGGGCTTATGATGCTACAATTGCATTAGCAATGGCTATTGAGGAAGTTGGAATTGGAGCCACTAAATTTGGTTACAATGAGAGTAACACATCAAGCAATTATTACATGCCTACTTTTGAAAATTTTGGTATTGCACAAAATGGTGAGAAATTGGGTCAAGCTTTGTTAAACACTAGATTTAATGGTCTTAGTGGTGATTTCAATGTTGTTGGTGGGAAGTTACAAGCATCAATATATGAAATAATTAATGTGATTGGTGATGGGGAAAAAAGGGTTGGATTTTGGACACCACATGAAGGGTTTGTTAGAAATTTGGACACAAAAGGCATAATAAGAAGCAATAATAGCATATACTCTACTTCTAAAAATGATCTTGGGGTAATCATGTGGCCAGGGGATATGAACTCTATTCCAAAGGGATGGGAAATTCCTACTATTGGGAAGAAGTTGAGGATAGGATTACCAGTGAAAAATGGAGATAACTACACTGAATTTTTGCATATAAGTACTAATTCAACACTAGCCACTGGCTTCTGCATTGATGTCTTCAAAGCTGTGGTGGAAATGTTGCCTTATGCATTACCATATGACTTTGTTCCATTTGCAAAGCCTGATGGTGAGATGGCGGGAACCTACAATGATTTGATCACTCAACTTTATTATGGGGTAATCTTTTTTCTCATATCAAGAACCCTGATCTTATATATGTGAATGATTTAATTTACATCATGAAACATTGCCAAATTCTTAGTATACTTTTAATATATGAAATATGTGAACTTTATGGTTTCTTTATTGGATAAATAATCAAATTAATGCATGAAATTGTAAGAAATGGTCAAAATAGTCCCTACTCTTTGAGAGTTTAAAAACACATCCATTAAATAATAAAATGTCATTTAAAATAATCATGCGTTAATTTTTGTTGTTAAAGACCGATGTGAGTGGCAACTGATTTGCCTATGGATTTTAGGTCTCCTAATGACTACTTTGTCAGTAATTAGTCTTTGAACAATTGAAATTACTAACAAATTGTTTTTGAAATAACCTAAATATCATAGGCTAACTAGTCCATGGTGATTTTGGATCAGAACTTTGATGCGGTGGTGGGGGATGTAACGATCATTGCAAACAGATCCGACTATGTTGATTTCACAATGCCATACACAGAATCTGGTGTGACAATGGTTGTTATAATGAAAGACAACAGAAAGAAAAATGCATGGGCCTTTTTGAAGCCACTCACATGGGATCTTTGGGTAACAACAGCTTGTTCATTTGTATTCATTGGATTTGTTGTATGGGTCCTTGAACATAGAATAAACAACGATTTCAGAGGACCAACCTCTCATCAGATTGGCACAAGCTTATGGTTTTCCTTTTCAACTATGGTCTTTGCTCAAAGTAAGTTTCACTCATCAAACACACACATTCATACAGTAAGCACATCAATGATTATCCGATCAAGATCGAACAATTCAGCTTTCAAACTCAGTATTTTAGAGTTAAAAAATATCAAAATTTACTTACTTTCTATATTGTACAATCTCGTTCCAAAAATTGCGGATTACTTGAATTTTTTGCTACATACAATCTAAATCCATAAGTGTTCATGTATAAGTTATTCTATCACAAATTAGGAAAGATTTAGGCATTGATGAGTTGGATCCAAATATGGTTTCTGATAAACTTTATGACGTAATTTGATCCATGCAGTTGATCCCACTTAGTAGTGGGATAAACTTTTTTCATATAAGTTAGAAGCTATTTTCAAAAGCTATCATGGAGTGCTTATAGAAATCTAGAGAAAATAGGTTATGGACATGTCATATGTTGTTTGCATGCTCTCTTAAAAAGTATCACAAGTATTTACATCGGTAGATAAGTTCAAATAAGTCAATTCAAATACGCTCTCACGGTTAACCATCACATTTTAATCACAGGAGAGAGAGTAGTGAGTAACTTGGCAAGATTTGTGGTGGTTATATGGGTTTTTGTGGTTCTAATTTTGGTTCAAAGTTACACTGCAAGCTTGACTTCACTCTTAACAGTTGAACAACTAAAGCCAGCTATTACAGACGTTAACCAACTTCTAAAGAATAAGATGAATGTTGGCTATCTGGAAGGTTCTTTTGTTTATGGAATCTTGAAGGAAATGAATTTCCAAGAATCTCAGCTCATTACTTATCAATCACCTGAAGAATGTAATGAGCTTTTCATAAAAGGAAGTGCAAATGGTGGTATTGATGCTGCTTTTGATGAAGTTCCCTATGTTAAGCAATTTCTTGGAATTTATAGTTGTTCTTCTTCAAAGTATGTCATGGTTGAGCCAAGATTTAAAACGGGTGGTTTTGGCTATGTAAGTTTTGTTAACTCTCTCTCTCATCTATCTCCCTAATATAATGTCATCATAATTTTCTCTTGTGAGCAACATTCCAAAATTCAAACCTAACATACTGATTCAATGAGTTGAATTGGTTTGATCCCAATTAATTGCAGTCTGGTTGAATTGAATTGGTTCAACTATGATTGGTTCAAACGATTCATGTTTTTATATTTTAATTTATTTCTTTTTACAATTTGAATACTATTTTACTTGTTTATTTATTTCTCATTTGGTTCACTTGTGATTGAACCAATTGAACTATAACCTAGAAGTCGCATTGGTTTGATTCACGATCGGGTTTGTAAAACATTGCTTGAGAGTACTAACTTGACTTTTGTGGGGGTGAATTTGTAGGCATTCCCAAAAGGTTCACCTCTGGTAGCAGACATTTCAAGGGCAATCCTAAATGTGACTCAAGGAGGCAAAATGAGAACAATTGAGAATGCATGGTTCAAAGAAAGTCGTTGTCTAGATTCCAATACAGAAATTTCCTCTAATAATAGTCTTGGCCTTGAAAGCTTCTGGGGTCTTTTTCTCATAGCAGGGATTGCTTCCCTATTAGCACTCCTAATCTTTGTGGTAACGTTTCTACATCAGCACAAACACATTTGGTTAAACAATAATCCAAGTAATTCAATATGGAGACGAATTGAAGTCATGGTTAGGATGTTTGATCAAAGAGACTTGAGCAGTCACACATTCAAGAAAACAGAAAATATAAATGGAAGTAGTAATAGTCCTTATCATGATGATGATTTAGGTGCGGTAGAGGCCTCTCCAGGTACACCTTATCCACCTAGTCCATTTAGTCAAACTGAATCAAATGTTTCCTTTTATGGAGATTTTAGTCAAAGCATAGAAATGGATGTCATGCAAATTACTAATCCAGAAGTTTCCCCTGTGAACAACTGTGAAATTAACTCCCAAGCAACACGAGATCATCTATAA
Protein sequence

>MS.gene32730.t1

MMSQGYVWIATDSMANLFNSLSYNVRESMEGVLGVRTYIPRTKKLEDFRVRWKRKFLSDNPTLVDTNLNIFGIWAYDATIALAMAIEEVGIGATKFGYNESNTSSNYYMPTFENFGIAQNGEKLGQALLNTRFNGLSGDFNVVGGKLQASIYEIINVIGDGEKRVGFWTPHEGFVRNLDTKGIIRSNNSIYSTSKNDLGVIMWPGDMNSIPKGWEIPTIGKKLRIGLPVKNGDNYTEFLHISTNSTLATGFCIDVFKAVVEMLPYALPYDFVPFAKPDGEMAGTYNDLITQLYYGNFDAVVGDVTIIANRSDYVDFTMPYTESGVTMVVIMKDNRKKNAWAFLKPLTWDLWVTTACSFVFIGFVVWVLEHRINNDFRGPTSHQIGTSLWFSFSTMVFAQRERVVSNLARFVVVIWVFVVLILVQSYTASLTSLLTVEQLKPAITDVNQLLKNKMNVGYLEGSFVYGILKEMNFQESQLITYQSPEECNELFIKGSANGGIDAAFDEVPYVKQFLGIYSCSSSKYVMVEPRFKTGGFGYAFPKGSPLVADISRAILNVTQGGKMRTIENAWFKESRCLDSNTEISSNNSLGLESFWGLFLIAGIASLLALLIFVVTFLHQHKHIWLNNNPSNSIWRRIEVMVRMFDQRDLSSHTFKKTENINGSSNSPYHDDDLGAVEASPGTPYPPSPFSQTESNVSFYGDFSQSIEMDVMQITNPEVSPVNNCEINSQATRDHL