Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32761.t1 | XP_003603204.2 | 85.2 | 263 | 38 | 1 | 1 | 262 | 7 | 269 | 1.40E-98 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32761.t1 | G7J8E8 | 85.2 | 263 | 38 | 1 | 1 | 262 | 1 | 263 | 1.0e-98 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052142 | MS.gene32761 | 0.840573 | 8.02E-58 | -1.69E-46 |
| MS.gene052187 | MS.gene32761 | 0.845571 | 3.74E-59 | -1.69E-46 |
| MS.gene053074 | MS.gene32761 | 0.837251 | 5.80E-57 | -1.69E-46 |
| MS.gene055359 | MS.gene32761 | 0.855922 | 4.56E-62 | -1.69E-46 |
| MS.gene058168 | MS.gene32761 | 0.810268 | 1.25E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene32761 | 0.821319 | 4.30E-53 | -1.69E-46 |
| MS.gene059632 | MS.gene32761 | 0.81936 | 1.21E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32761.t1 | MTR_3g105010 | 94.297 | 263 | 14 | 1 | 1 | 262 | 1 | 263 | 3.90e-177 | 488 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAACAACAAACACATTCTT+TGG | 0.164595 | 3.1:-83002200 | MS.gene32761:CDS |
| TGGTTTGGGGTTAAAATATT+TGG | 0.176425 | 3.1:+83002364 | None:intergenic |
| GATGCGAAAGAAGAGATTTA+AGG | 0.234830 | 3.1:-83001524 | MS.gene32761:CDS |
| GGATACAGAGGGAAGAAAAT+TGG | 0.293718 | 3.1:-83001387 | MS.gene32761:CDS |
| TTCTTGATAAAGGATGTTAT+TGG | 0.304540 | 3.1:+83002429 | None:intergenic |
| TGAGTTTCTGTTCTTGATAA+AGG | 0.309215 | 3.1:+83002419 | None:intergenic |
| TAGGGTGATGAATGTGGTTT+GGG | 0.329828 | 3.1:+83002350 | None:intergenic |
| TCCAGCATTGAGAAAATAAC+TGG | 0.333676 | 3.1:-83001274 | MS.gene32761:CDS |
| AATGATAGTGTTGGAGAATA+GGG | 0.371956 | 3.1:+83002332 | None:intergenic |
| ATTTGGAGGGTTGTTGAGAA+TGG | 0.396091 | 3.1:+83002381 | None:intergenic |
| GGAAAACAACGAGAGATGTT+TGG | 0.402412 | 3.1:-83001647 | MS.gene32761:CDS |
| TTGGTGATTAACCACAGTTT+TGG | 0.419669 | 3.1:+83001462 | None:intergenic |
| TAATGATAGTGTTGGAGAAT+AGG | 0.423149 | 3.1:+83002331 | None:intergenic |
| GGTTCTTCAGAAGAAATTGT+TGG | 0.438181 | 3.1:-83001437 | MS.gene32761:CDS |
| CAACAACAAACACATTCTTT+GGG | 0.448951 | 3.1:-83002199 | MS.gene32761:CDS |
| TGAGAATGGAACCATCATCT+AGG | 0.456014 | 3.1:+83002395 | None:intergenic |
| TTGGTTTCCGACAACAACTT+TGG | 0.462012 | 3.1:-83001628 | MS.gene32761:CDS |
| ATAGGGTGATGAATGTGGTT+TGG | 0.467705 | 3.1:+83002349 | None:intergenic |
| GTTGGAGGTGATTGATAGAA+TGG | 0.468582 | 3.1:-83001419 | MS.gene32761:CDS |
| ATTTCTTCTGAAGAACCTCT+TGG | 0.473081 | 3.1:+83001443 | None:intergenic |
| AAGCTCACCAAAGTTGTTGT+CGG | 0.473825 | 3.1:+83001621 | None:intergenic |
| TGTGTTTGTTGTTGTTGTGA+TGG | 0.481062 | 3.1:+83002208 | None:intergenic |
| CTTGAAGCTGTGTACAAACT+TGG | 0.499197 | 3.1:-83001601 | MS.gene32761:CDS |
| GTGATGGTTGAAATTTATGC+TGG | 0.505292 | 3.1:+83002224 | None:intergenic |
| TTTGGGGTTAAAATATTTGG+AGG | 0.507238 | 3.1:+83002367 | None:intergenic |
| AGGAACATGATTCGAAGAGA+AGG | 0.540619 | 3.1:-83001558 | MS.gene32761:CDS |
| CAGAAACTCACCCTAGATGA+TGG | 0.541007 | 3.1:-83002406 | MS.gene32761:CDS |
| ATGATTCGAAGAGAAGGGCT+AGG | 0.550539 | 3.1:-83001552 | MS.gene32761:CDS |
| TTGGGGTTAAAATATTTGGA+GGG | 0.566517 | 3.1:+83002368 | None:intergenic |
| TCTTCAGAAGAAATTGTTGG+AGG | 0.567079 | 3.1:-83001434 | MS.gene32761:CDS |
| GAGAATGGAACCATCATCTA+GGG | 0.574060 | 3.1:+83002396 | None:intergenic |
| GGAACATGATTCGAAGAGAA+GGG | 0.586343 | 3.1:-83001557 | MS.gene32761:CDS |
| GGAGAATAGGGTGATGAATG+TGG | 0.595952 | 3.1:+83002344 | None:intergenic |
| TAAAAGATTGACCAAAACTG+TGG | 0.602456 | 3.1:-83001473 | MS.gene32761:CDS |
| TACAGAGGGAAGAAAATTGG+AGG | 0.605765 | 3.1:-83001384 | MS.gene32761:CDS |
| CAGGAAAGCATTCAACAACA+CGG | 0.615004 | 3.1:-83001707 | MS.gene32761:CDS |
| AATCGTTACATCGAATGCGA+AGG | 0.628296 | 3.1:-83002163 | MS.gene32761:CDS |
| ACACACAACAACAGAAAGAA+CGG | 0.629520 | 3.1:-83002291 | MS.gene32761:CDS |
| ATTGTGAGTAATGATAGTGT+TGG | 0.653355 | 3.1:+83002323 | None:intergenic |
| AACTGTGGTTAATCACCAAG+AGG | 0.665729 | 3.1:-83001458 | MS.gene32761:CDS |
| AGGGTGATGAATGTGGTTTG+GGG | 0.692532 | 3.1:+83002351 | None:intergenic |
| TCAGAATCAAGCGTTCACTG+AGG | 0.786263 | 3.1:-83001578 | MS.gene32761:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATTAAAAATATTTATCAA+GGG | + | chr3.1:83001874-83001893 | None:intergenic | 10.0% |
| !! | GTCATTAAAAATATTTATCA+AGG | + | chr3.1:83001875-83001894 | None:intergenic | 15.0% |
| ! | ACAAACATACATAATGTGAT+AGG | + | chr3.1:83001950-83001969 | None:intergenic | 25.0% |
| ! | AGAAAAACAACAACATGAAA+AGG | - | chr3.1:83002020-83002039 | MS.gene32761:intron | 25.0% |
| ! | CAGAACATGTTATATAATGT+GGG | + | chr3.1:83001674-83001693 | None:intergenic | 25.0% |
| ! | TCAGAACATGTTATATAATG+TGG | + | chr3.1:83001675-83001694 | None:intergenic | 25.0% |
| ! | TTCTTGATAAAGGATGTTAT+TGG | + | chr3.1:83001262-83001281 | None:intergenic | 25.0% |
| ! | TTGATAGAATGGAAAAAGAA+AGG | - | chr3.1:83002280-83002299 | MS.gene32761:CDS | 25.0% |
| !! | AATATTTATCAAGGGTTGAT+AGG | + | chr3.1:83001866-83001885 | None:intergenic | 25.0% |
| !! | ACATTTTTATCAATCACATG+AGG | - | chr3.1:83001437-83001456 | MS.gene32761:CDS | 25.0% |
| ACAACAACAAACACATTCTT+TGG | - | chr3.1:83001488-83001507 | MS.gene32761:CDS | 30.0% | |
| CAACAACAAACACATTCTTT+GGG | - | chr3.1:83001489-83001508 | MS.gene32761:CDS | 30.0% | |
| CACATTATGTATGTTTGTGT+TGG | - | chr3.1:83001952-83001971 | MS.gene32761:intron | 30.0% | |
| CAGATTTGTACATTTATTCC+GGG | + | chr3.1:83001713-83001732 | None:intergenic | 30.0% | |
| CCAGATTTGTACATTTATTC+CGG | + | chr3.1:83001714-83001733 | None:intergenic | 30.0% | |
| GAATTACCAAAAAGATACTC+TGG | + | chr3.1:83001559-83001578 | None:intergenic | 30.0% | |
| TAAAAGATTGACCAAAACTG+TGG | - | chr3.1:83002215-83002234 | MS.gene32761:CDS | 30.0% | |
| TGAGTTTCTGTTCTTGATAA+AGG | + | chr3.1:83001272-83001291 | None:intergenic | 30.0% | |
| TTGGGGTTAAAATATTTGGA+GGG | + | chr3.1:83001323-83001342 | None:intergenic | 30.0% | |
| ! | AATGATAGTGTTGGAGAATA+GGG | + | chr3.1:83001359-83001378 | None:intergenic | 30.0% |
| ! | ATTGTGAGTAATGATAGTGT+TGG | + | chr3.1:83001368-83001387 | None:intergenic | 30.0% |
| ! | TAATGATAGTGTTGGAGAAT+AGG | + | chr3.1:83001360-83001379 | None:intergenic | 30.0% |
| ! | TGGTTTGGGGTTAAAATATT+TGG | + | chr3.1:83001327-83001346 | None:intergenic | 30.0% |
| ! | TTTGGGGTTAAAATATTTGG+AGG | + | chr3.1:83001324-83001343 | None:intergenic | 30.0% |
| ACAAGACCAATACATCAAGA+TGG | + | chr3.1:83001775-83001794 | None:intergenic | 35.0% | |
| ACACACAACAACAGAAAGAA+CGG | - | chr3.1:83001397-83001416 | MS.gene32761:CDS | 35.0% | |
| CAAGACCAATACATCAAGAT+GGG | + | chr3.1:83001774-83001793 | None:intergenic | 35.0% | |
| CCGGAATAAATGTACAAATC+TGG | - | chr3.1:83001711-83001730 | MS.gene32761:intron | 35.0% | |
| GATGCGAAAGAAGAGATTTA+AGG | - | chr3.1:83002164-83002183 | MS.gene32761:CDS | 35.0% | |
| GCAATTAGTACAGGTGATAT+AGG | - | chr3.1:83001825-83001844 | MS.gene32761:intron | 35.0% | |
| GGAAAAAGAAAGGATACAGA+GGG | - | chr3.1:83002290-83002309 | MS.gene32761:CDS | 35.0% | |
| GGTTCTTCAGAAGAAATTGT+TGG | - | chr3.1:83002251-83002270 | MS.gene32761:CDS | 35.0% | |
| GTGATGGTTGAAATTTATGC+TGG | + | chr3.1:83001467-83001486 | None:intergenic | 35.0% | |
| TACTACCCATCTTGATGTAT+TGG | - | chr3.1:83001766-83001785 | MS.gene32761:intron | 35.0% | |
| TATAATGTGGGTGTATATGC+TGG | + | chr3.1:83001662-83001681 | None:intergenic | 35.0% | |
| TCTTCAGAAGAAATTGTTGG+AGG | - | chr3.1:83002254-83002273 | MS.gene32761:CDS | 35.0% | |
| TGGAAAAAGAAAGGATACAG+AGG | - | chr3.1:83002289-83002308 | MS.gene32761:CDS | 35.0% | |
| ! | ATTTCTTCTGAAGAACCTCT+TGG | + | chr3.1:83002248-83002267 | None:intergenic | 35.0% |
| ! | TCCAGCATTGAGAAAATAAC+TGG | - | chr3.1:83002414-83002433 | MS.gene32761:CDS | 35.0% |
| !! | ACCAGTTATTTTCTCAATGC+TGG | + | chr3.1:83002418-83002437 | None:intergenic | 35.0% |
| !! | ATGTTTGTGTTGGCATTTTC+AGG | - | chr3.1:83001962-83001981 | MS.gene32761:intron | 35.0% |
| !! | TGTGTTTGTTGTTGTTGTGA+TGG | + | chr3.1:83001483-83001502 | None:intergenic | 35.0% |
| !! | TTGGTGATTAACCACAGTTT+TGG | + | chr3.1:83002229-83002248 | None:intergenic | 35.0% |
| AACTGTGGTTAATCACCAAG+AGG | - | chr3.1:83002230-83002249 | MS.gene32761:CDS | 40.0% | |
| AAGCTCACCAAAGTTGTTGT+CGG | + | chr3.1:83002070-83002089 | None:intergenic | 40.0% | |
| AATCGTTACATCGAATGCGA+AGG | - | chr3.1:83001525-83001544 | MS.gene32761:CDS | 40.0% | |
| AGGAACATGATTCGAAGAGA+AGG | - | chr3.1:83002130-83002149 | MS.gene32761:intron | 40.0% | |
| ATAGGGTGATGAATGTGGTT+TGG | + | chr3.1:83001342-83001361 | None:intergenic | 40.0% | |
| ATTTGGAGGGTTGTTGAGAA+TGG | + | chr3.1:83001310-83001329 | None:intergenic | 40.0% | |
| CCAAAAAGATACTCTGGCAT+TGG | + | chr3.1:83001553-83001572 | None:intergenic | 40.0% | |
| CTTGAAGCTGTGTACAAACT+TGG | - | chr3.1:83002087-83002106 | MS.gene32761:intron | 40.0% | |
| GAGAATGGAACCATCATCTA+GGG | + | chr3.1:83001295-83001314 | None:intergenic | 40.0% | |
| GGAAAACAACGAGAGATGTT+TGG | - | chr3.1:83002041-83002060 | MS.gene32761:intron | 40.0% | |
| GGAACATGATTCGAAGAGAA+GGG | - | chr3.1:83002131-83002150 | MS.gene32761:intron | 40.0% | |
| GGATACAGAGGGAAGAAAAT+TGG | - | chr3.1:83002301-83002320 | MS.gene32761:CDS | 40.0% | |
| GTTGGAGGTGATTGATAGAA+TGG | - | chr3.1:83002269-83002288 | MS.gene32761:CDS | 40.0% | |
| TACAGAGGGAAGAAAATTGG+AGG | - | chr3.1:83002304-83002323 | MS.gene32761:CDS | 40.0% | |
| TAGGGTGATGAATGTGGTTT+GGG | + | chr3.1:83001341-83001360 | None:intergenic | 40.0% | |
| TGAGAATGGAACCATCATCT+AGG | + | chr3.1:83001296-83001315 | None:intergenic | 40.0% | |
| TTCGCTTTCGCAATTAGTAC+AGG | - | chr3.1:83001816-83001835 | MS.gene32761:intron | 40.0% | |
| TTGGTTTCCGACAACAACTT+TGG | - | chr3.1:83002060-83002079 | MS.gene32761:intron | 40.0% | |
| TTTATTCCGGGCTCATTGTA+CGG | + | chr3.1:83001701-83001720 | None:intergenic | 40.0% | |
| ! | GTACAAATCTGGTTAGCTCA+TGG | - | chr3.1:83001722-83001741 | MS.gene32761:intron | 40.0% |
| ! | GTGAGCGTGATTTTGCTAAA+CGG | - | chr3.1:83002373-83002392 | MS.gene32761:CDS | 40.0% |
| !! | CAGGAAAGCATTCAACAACA+CGG | - | chr3.1:83001981-83002000 | MS.gene32761:intron | 40.0% |
| !! | CCAATGCCAGAGTATCTTTT+TGG | - | chr3.1:83001550-83001569 | MS.gene32761:CDS | 40.0% |
| AGGGTGATGAATGTGGTTTG+GGG | + | chr3.1:83001340-83001359 | None:intergenic | 45.0% | |
| ATGATTCGAAGAGAAGGGCT+AGG | - | chr3.1:83002136-83002155 | MS.gene32761:intron | 45.0% | |
| CAGAAACTCACCCTAGATGA+TGG | - | chr3.1:83001282-83001301 | MS.gene32761:CDS | 45.0% | |
| GGAGAATAGGGTGATGAATG+TGG | + | chr3.1:83001347-83001366 | None:intergenic | 45.0% | |
| TCAGAATCAAGCGTTCACTG+AGG | - | chr3.1:83002110-83002129 | MS.gene32761:intron | 45.0% | |
| TGATAACCGTACAATGAGCC+CGG | - | chr3.1:83001692-83001711 | MS.gene32761:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 83001255 | 83002455 | 83001255 | ID=MS.gene32761 |
| chr3.1 | mRNA | 83001255 | 83002455 | 83001255 | ID=MS.gene32761.t1;Parent=MS.gene32761 |
| chr3.1 | exon | 83002139 | 83002455 | 83002139 | ID=MS.gene32761.t1.exon1;Parent=MS.gene32761.t1 |
| chr3.1 | CDS | 83002139 | 83002455 | 83002139 | ID=cds.MS.gene32761.t1;Parent=MS.gene32761.t1 |
| chr3.1 | exon | 83001255 | 83001726 | 83001255 | ID=MS.gene32761.t1.exon2;Parent=MS.gene32761.t1 |
| chr3.1 | CDS | 83001255 | 83001726 | 83001255 | ID=cds.MS.gene32761.t1;Parent=MS.gene32761.t1 |
| Gene Sequence |
| Protein sequence |
