Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32888.t1 | XP_003614705.1 | 94 | 84 | 5 | 0 | 9 | 92 | 843 | 926 | 6.50E-35 | 156.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32888.t1 | Q9S7Y7 | 43.5 | 85 | 44 | 3 | 9 | 92 | 832 | 913 | 4.3e-11 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32888.t1 | G7JXA7 | 94.0 | 84 | 5 | 0 | 9 | 92 | 843 | 926 | 4.7e-35 | 156.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene32888 | 0.825525 | 4.47E-54 | -1.69E-46 |
| MS.gene051719 | MS.gene32888 | 0.80197 | 7.00E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene32888 | 0.803316 | 3.70E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene32888 | 0.84579 | 3.26E-59 | -1.69E-46 |
| MS.gene052665 | MS.gene32888 | 0.800561 | 1.36E-48 | -1.69E-46 |
| MS.gene053581 | MS.gene32888 | 0.802508 | 5.43E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene32888 | 0.811306 | 7.48E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene32888 | 0.812334 | 4.47E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene32888 | 0.82366 | 1.23E-53 | -1.69E-46 |
| MS.gene055808 | MS.gene32888 | 0.807999 | 3.84E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene32888 | 0.814403 | 1.57E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene32888 | 0.802943 | 4.41E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene32888 | 0.809841 | 1.55E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene32888 | 0.816964 | 4.21E-52 | -1.69E-46 |
| MS.gene060587 | MS.gene32888 | 0.822642 | 2.12E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32888.t1 | MTR_5g057990 | 94.048 | 84 | 5 | 0 | 9 | 92 | 843 | 926 | 1.47e-47 | 161 |
| MS.gene32888.t1 | MTR_1g111000 | 55.435 | 92 | 37 | 1 | 1 | 92 | 850 | 937 | 5.43e-28 | 105 |
| MS.gene32888.t1 | MTR_1g110980 | 52.381 | 84 | 35 | 2 | 9 | 92 | 846 | 924 | 2.49e-23 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32888.t1 | AT1G68560 | 43.529 | 85 | 44 | 3 | 9 | 92 | 832 | 913 | 9.96e-16 | 70.9 |
| MS.gene32888.t1 | AT3G45940 | 39.286 | 84 | 48 | 3 | 11 | 92 | 771 | 853 | 4.46e-12 | 60.5 |
Find 19 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGCTATGACATGGAAAA+TGG | 0.163449 | 3.1:-81465159 | MS.gene32888:CDS |
| TTAGATGGAAAACCACTAAT+TGG | 0.283753 | 3.1:-81465299 | MS.gene32888:CDS |
| TACTTCTGAACATGTGCATT+TGG | 0.366445 | 3.1:-81465255 | MS.gene32888:CDS |
| GTGGCTTCACTTGAGTTAGA+TGG | 0.373357 | 3.1:-81465314 | MS.gene32888:CDS |
| ACACTTGACAAGCCAATTAG+TGG | 0.412039 | 3.1:+81465287 | None:intergenic |
| GCAAGGTGGATTGATCTCTA+AGG | 0.447708 | 3.1:-81465405 | MS.gene32888:CDS |
| TTTGCTATGACATGGAAAAT+GGG | 0.511308 | 3.1:-81465158 | MS.gene32888:CDS |
| TCTAAGGATGCTTTAGACAA+AGG | 0.534929 | 3.1:-81465389 | MS.gene32888:CDS |
| AGATGGAAGTGTTGGAGCAG+TGG | 0.546659 | 3.1:-81465333 | MS.gene32888:CDS |
| TTGGGATTAGATGGAAGTGT+TGG | 0.571364 | 3.1:-81465341 | MS.gene32888:CDS |
| CATTTGGAAGGTGAAGGAAA+TGG | 0.600808 | 3.1:-81465239 | MS.gene32888:CDS |
| TCTGAACATGTGCATTTGGA+AGG | 0.603305 | 3.1:-81465251 | MS.gene32888:CDS |
| ACTGTGATGGTAACTTTGAG+AGG | 0.604296 | 3.1:-81465206 | MS.gene32888:CDS |
| AGAGGGTTGAGCATTCCTGT+TGG | 0.649091 | 3.1:-81465188 | MS.gene32888:CDS |
| CTGTGATGGTAACTTTGAGA+GGG | 0.681039 | 3.1:-81465205 | MS.gene32888:CDS |
| TGGTGAAAGTAAGACTGTGA+TGG | 0.681480 | 3.1:-81465219 | MS.gene32888:CDS |
| TGATCTCTAAGGATGCAGCA+AGG | 0.697333 | 3.1:-81465422 | None:intergenic |
| TCTCTAAGGATGCAGCAAGG+TGG | 0.705537 | 3.1:-81465419 | None:intergenic |
| CATGTGCATTTGGAAGGTGA+AGG | 0.717327 | 3.1:-81465245 | MS.gene32888:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATTCAATTTTTGTGTT+GGG | - | chr3.1:81465205-81465224 | MS.gene32888:CDS | 20.0% |
| !!! | TATTGATTCAATTTTTGTGT+TGG | - | chr3.1:81465204-81465223 | MS.gene32888:CDS | 20.0% |
| TTAGATGGAAAACCACTAAT+TGG | - | chr3.1:81465265-81465284 | MS.gene32888:CDS | 30.0% | |
| ! | ATAGCAAAGTTTTTACCAAC+AGG | + | chr3.1:81465394-81465413 | None:intergenic | 30.0% |
| ! | ATTTTTGTGTTGGGATTAGA+TGG | - | chr3.1:81465214-81465233 | MS.gene32888:CDS | 30.0% |
| ! | GTAAAAACTTTGCTATGACA+TGG | - | chr3.1:81465398-81465417 | MS.gene32888:CDS | 30.0% |
| ! | TTTGCTATGACATGGAAAAT+GGG | - | chr3.1:81465406-81465425 | MS.gene32888:CDS | 30.0% |
| TACTTCTGAACATGTGCATT+TGG | - | chr3.1:81465309-81465328 | MS.gene32888:CDS | 35.0% | |
| ! | CTTTGCTATGACATGGAAAA+TGG | - | chr3.1:81465405-81465424 | MS.gene32888:CDS | 35.0% |
| ! | TCTAAGGATGCTTTAGACAA+AGG | - | chr3.1:81465175-81465194 | MS.gene32888:CDS | 35.0% |
| ACACTTGACAAGCCAATTAG+TGG | + | chr3.1:81465280-81465299 | None:intergenic | 40.0% | |
| ACTGTGATGGTAACTTTGAG+AGG | - | chr3.1:81465358-81465377 | MS.gene32888:CDS | 40.0% | |
| CATTTGGAAGGTGAAGGAAA+TGG | - | chr3.1:81465325-81465344 | MS.gene32888:CDS | 40.0% | |
| TCTGAACATGTGCATTTGGA+AGG | - | chr3.1:81465313-81465332 | MS.gene32888:CDS | 40.0% | |
| TGGTGAAAGTAAGACTGTGA+TGG | - | chr3.1:81465345-81465364 | MS.gene32888:CDS | 40.0% | |
| ! | CTGTGATGGTAACTTTGAGA+GGG | - | chr3.1:81465359-81465378 | MS.gene32888:CDS | 40.0% |
| ! | TTGGGATTAGATGGAAGTGT+TGG | - | chr3.1:81465223-81465242 | MS.gene32888:CDS | 40.0% |
| CATGTGCATTTGGAAGGTGA+AGG | - | chr3.1:81465319-81465338 | MS.gene32888:CDS | 45.0% | |
| GTGGCTTCACTTGAGTTAGA+TGG | - | chr3.1:81465250-81465269 | MS.gene32888:CDS | 45.0% | |
| !! | GCAAGGTGGATTGATCTCTA+AGG | - | chr3.1:81465159-81465178 | MS.gene32888:CDS | 45.0% |
| AGAGGGTTGAGCATTCCTGT+TGG | - | chr3.1:81465376-81465395 | MS.gene32888:CDS | 50.0% | |
| !! | AGATGGAAGTGTTGGAGCAG+TGG | - | chr3.1:81465231-81465250 | MS.gene32888:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81465154 | 81465432 | 81465154 | ID=MS.gene32888 |
| chr3.1 | mRNA | 81465154 | 81465432 | 81465154 | ID=MS.gene32888.t1;Parent=MS.gene32888 |
| chr3.1 | exon | 81465154 | 81465432 | 81465154 | ID=MS.gene32888.t1.exon1;Parent=MS.gene32888.t1 |
| chr3.1 | CDS | 81465154 | 81465432 | 81465154 | ID=cds.MS.gene32888.t1;Parent=MS.gene32888.t1 |
| Gene Sequence |
| Protein sequence |
