AlfalfaGEDB | MS.gene33159

M. sativa cultivar XinJiangDaYe / MS.gene33159

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33159.t1	XP_013465831.1	97	263	7	1	1	263	1	262	6.00E-134	486.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33159.t1	Q39262	49.4	172	66	7	51	222	18	168	2.6e-29	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33159.t1	A0A072VDM2	97.0	263	7	1	1	263	1	262	4.3e-134	486.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057148	MS.gene33159	-0.804449	2.15E-49	-1.69E-46
MS.gene05985	MS.gene33159	-0.803185	3.93E-49	-1.69E-46
MS.gene059901	MS.gene33159	-0.802522	5.39E-49	-1.69E-46
MS.gene060512	MS.gene33159	0.800754	1.24E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33159.t1	MTR_1g013130	96.958	263	7	1	1	263	1	262	0.0	532
MS.gene33159.t1	MTR_3g106230	48.729	236	97	9	23	248	4	225	1.46e-57	183
MS.gene33159.t1	MTR_2g104900	35.563	284	125	13	1	263	1	247	2.08e-31	117
MS.gene33159.t1	MTR_4g006575	37.553	237	96	10	46	263	21	224	8.31e-31	114
MS.gene33159.t1	MTR_4g006600	37.553	237	96	10	46	263	21	224	8.31e-31	114
MS.gene33159.t1	MTR_5g077370	34.975	203	86	6	31	211	2	180	1.20e-25	101
MS.gene33159.t1	MTR_1g033340	38.095	147	70	3	35	181	43	168	2.37e-24	98.2
MS.gene33159.t1	MTR_3g065020	54.545	88	22	2	93	180	85	154	6.88e-24	96.7
MS.gene33159.t1	MTR_1g107055	37.594	133	59	3	53	182	21	132	1.29e-18	81.6
MS.gene33159.t1	MTR_4g128340	61.538	52	20	0	91	142	42	93	4.18e-16	74.3
MS.gene33159.t1	MTR_3g011990	30.645	186	97	6	28	198	20	188	7.99e-16	75.9
MS.gene33159.t1	MTR_3g012010	41.176	102	50	3	85	185	89	181	2.26e-14	72.0
MS.gene33159.t1	MTR_3g011970	33.333	186	98	7	28	198	20	194	2.33e-14	71.6
MS.gene33159.t1	MTR_8g466760	37.963	108	51	3	92	198	96	188	6.65e-14	70.5
MS.gene33159.t1	MTR_5g008280	38.793	116	56	4	82	195	34	136	1.82e-13	69.7
MS.gene33159.t1	MTR_5g008310	40.187	107	45	5	91	191	69	162	2.17e-13	69.3
MS.gene33159.t1	MTR_5g008320	38.889	108	45	5	91	191	69	162	4.02e-13	68.6
MS.gene33159.t1	MTR_5g008390	36.036	111	41	3	86	192	6	90	7.90e-13	63.2
MS.gene33159.t1	MTR_7g083210	78.788	33	7	0	94	126	39	71	8.99e-13	65.5
MS.gene33159.t1	MTR_7g086860	70.270	37	11	0	94	130	30	66	1.21e-12	64.3
MS.gene33159.t1	MTR_7g022980	36.441	118	62	5	83	195	55	164	3.34e-12	65.5
MS.gene33159.t1	MTR_3g009410	40.000	95	41	4	92	182	96	178	1.58e-11	63.5
MS.gene33159.t1	MTR_7g026977	32.184	174	102	8	27	198	86	245	2.39e-11	63.5
MS.gene33159.t1	MTR_2g067740	35.238	105	53	2	92	195	57	147	4.28e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33159.t1	AT5G10970	42.616	237	90	9	49	263	60	272	4.04e-44	150
MS.gene33159.t1	AT5G25160	49.419	172	66	7	51	222	18	168	2.83e-39	137
MS.gene33159.t1	AT1G80730	37.395	238	127	7	35	263	4	228	1.90e-32	119
MS.gene33159.t1	AT1G66140	49.515	103	35	3	91	183	80	175	5.67e-22	92.0
MS.gene33159.t1	AT1G24625	42.727	110	37	4	74	179	33	120	1.23e-16	76.6
MS.gene33159.t1	AT5G14010	42.697	89	39	3	94	178	36	116	3.81e-14	68.9
MS.gene33159.t1	AT5G57520	77.778	36	8	0	94	129	50	85	5.01e-14	68.2
MS.gene33159.t1	AT1G67030	38.938	113	55	3	70	180	13	113	3.34e-13	67.0
MS.gene33159.t1	AT3G58070	31.638	177	92	7	23	190	23	179	6.38e-12	64.3
MS.gene33159.t1	AT5G01860	56.250	48	18	1	80	127	54	98	7.33e-11	60.8

Find 31 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCAAACTCAAGAATCTTTA+TGG	0.122503	1.3:+8696697	MS.gene33159:CDS
ATGGCTTCTTTGCCTCTTTA+TGG	0.168485	1.3:+8696557	MS.gene33159:CDS
CTTTGTTTATTATGCATAAT+AGG	0.201649	1.3:-8696524	None:intergenic
CCTTCTCATGTTTCATCTAA+TGG	0.202335	1.3:+8696635	MS.gene33159:CDS
CATGTTTCATCTAATGGATT+TGG	0.228057	1.3:+8696641	MS.gene33159:CDS
AATGGATTTGGAAACTCTTA+TGG	0.229174	1.3:+8696653	MS.gene33159:CDS
ATAGCTTCAGCTTCTGCTTT+TGG	0.233602	1.3:+8696497	MS.gene33159:CDS
TTGCCTCTTTATGGTGCTTT+TGG	0.261823	1.3:+8696566	MS.gene33159:CDS
ATGAAACATGAGAAGGCTTA+TGG	0.351379	1.3:-8696628	None:intergenic
CTCATAACTTCTTTAGATGT+TGG	0.385754	1.3:+8696305	MS.gene33159:CDS
TGCAAAGGTGTGCTTGGACT+TGG	0.428789	1.3:-8696143	None:intergenic
TTCTATAGCTCACAAGCATT+AGG	0.451109	1.3:+8696404	MS.gene33159:CDS
TTTGGTGGTAATAAACCTCT+TGG	0.453972	1.3:+8696584	MS.gene33159:CDS
AAAGGTGTGCTTGGACTTGG+AGG	0.516654	1.3:-8696140	None:intergenic
TGGAATAGCAAAACTAGGTA+TGG	0.517898	1.3:+8696730	MS.gene33159:CDS
ATCTTTATGGATCAACAACC+TGG	0.518646	1.3:+8696710	MS.gene33159:CDS
CAACCTGGAATAGCAAAACT+AGG	0.529841	1.3:+8696725	MS.gene33159:CDS
CCACCAAAAGCACCATAAAG+AGG	0.536698	1.3:-8696569	None:intergenic
CATCAAGAGGAAGTGTTGGT+AGG	0.548091	1.3:+8696777	MS.gene33159:CDS
GAAGAAACAACAATCTATTG+AGG	0.558110	1.3:+8696181	MS.gene33159:CDS
ATTAATGGAAAGACTAAGCA+AGG	0.563627	1.3:+8696088	None:intergenic
TATGGACATCATCATCATCA+TGG	0.564611	1.3:+8696671	MS.gene33159:CDS
TCTTGAGAAGAGAAGATGCA+TGG	0.579539	1.3:-8696113	None:intergenic
GGTAGAAGAAGATATCACTA+AGG	0.583712	1.3:+8696202	MS.gene33159:CDS
ACATCATCAAGAGGAAGTGT+TGG	0.585725	1.3:+8696773	MS.gene33159:CDS
TATAGCTCACAAGCATTAGG+TGG	0.606344	1.3:+8696407	MS.gene33159:CDS
AGAACAACAACATCATCAAG+AGG	0.611847	1.3:+8696764	MS.gene33159:CDS
ATATTCATAATCATAGCTTG+AGG	0.612882	1.3:-8696871	None:intergenic
TAAACAAAGTTATGCAAACA+TGG	0.616969	1.3:+8696538	MS.gene33159:CDS
CCATTAGATGAAACATGAGA+AGG	0.626125	1.3:-8696635	None:intergenic
GAGTGTGCTTGAATTCCAAG+AGG	0.647089	1.3:-8696599	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTGTTTATTATGCATAAT+AGG	-	chr1.3:8696527-8696546	None:intergenic	20.0%
!	TAAACAAAGTTATGCAAACA+TGG	+	chr1.3:8696538-8696557	MS.gene33159:CDS	25.0%
!!!	TTTTCATGTTGATTTTGCAA+AGG	-	chr1.3:8696161-8696180	None:intergenic	25.0%
	CTCATAACTTCTTTAGATGT+TGG	+	chr1.3:8696305-8696324	MS.gene33159:CDS	30.0%
	GAAGAAACAACAATCTATTG+AGG	+	chr1.3:8696181-8696200	MS.gene33159:CDS	30.0%
	GTCAAACTCAAGAATCTTTA+TGG	+	chr1.3:8696697-8696716	MS.gene33159:CDS	30.0%
!	AATGGATTTGGAAACTCTTA+TGG	+	chr1.3:8696653-8696672	MS.gene33159:CDS	30.0%
!!	CATGTTTCATCTAATGGATT+TGG	+	chr1.3:8696641-8696660	MS.gene33159:CDS	30.0%
	AGAACAACAACATCATCAAG+AGG	+	chr1.3:8696764-8696783	MS.gene33159:CDS	35.0%
	ATCTTTATGGATCAACAACC+TGG	+	chr1.3:8696710-8696729	MS.gene33159:CDS	35.0%
	ATGAAACATGAGAAGGCTTA+TGG	-	chr1.3:8696631-8696650	None:intergenic	35.0%
	CCATTAGATGAAACATGAGA+AGG	-	chr1.3:8696638-8696657	None:intergenic	35.0%
	CCTTCTCATGTTTCATCTAA+TGG	+	chr1.3:8696635-8696654	MS.gene33159:CDS	35.0%
	GAGAGATCAATAGCAAAAAG+AGG	+	chr1.3:8696452-8696471	MS.gene33159:CDS	35.0%
	GCAAAAAGAGGACAAAGATT+TGG	+	chr1.3:8696464-8696483	MS.gene33159:CDS	35.0%
	GGTAGAAGAAGATATCACTA+AGG	+	chr1.3:8696202-8696221	MS.gene33159:CDS	35.0%
	TATGGACATCATCATCATCA+TGG	+	chr1.3:8696671-8696690	MS.gene33159:CDS	35.0%
	TGGAATAGCAAAACTAGGTA+TGG	+	chr1.3:8696730-8696749	MS.gene33159:CDS	35.0%
	TTCTATAGCTCACAAGCATT+AGG	+	chr1.3:8696404-8696423	MS.gene33159:CDS	35.0%
!	TCTCTCTTTTGTGAGCATTT+TGG	-	chr1.3:8696436-8696455	None:intergenic	35.0%
!	TTTGGTGGTAATAAACCTCT+TGG	+	chr1.3:8696584-8696603	MS.gene33159:CDS	35.0%
!!!	ATACCTAGTTTTGCTATTCC+AGG	-	chr1.3:8696731-8696750	None:intergenic	35.0%
	ATGGCTTCTTTGCCTCTTTA+TGG	+	chr1.3:8696557-8696576	MS.gene33159:CDS	40.0%
	CAACCTGGAATAGCAAAACT+AGG	+	chr1.3:8696725-8696744	MS.gene33159:CDS	40.0%
	GACATCATCATCATCATGGT+TGG	+	chr1.3:8696675-8696694	MS.gene33159:CDS	40.0%
	TATAGCTCACAAGCATTAGG+TGG	+	chr1.3:8696407-8696426	MS.gene33159:CDS	40.0%
	TCTTGAGAAGAGAAGATGCA+TGG	-	chr1.3:8696116-8696135	None:intergenic	40.0%
!	ACATCATCAAGAGGAAGTGT+TGG	+	chr1.3:8696773-8696792	MS.gene33159:CDS	40.0%
!	ATAGCTTCAGCTTCTGCTTT+TGG	+	chr1.3:8696497-8696516	MS.gene33159:CDS	40.0%
!	TTGCCTCTTTATGGTGCTTT+TGG	+	chr1.3:8696566-8696585	MS.gene33159:CDS	40.0%
!!	TGATTTTGCAAAGGTGTGCT+TGG	-	chr1.3:8696152-8696171	None:intergenic	40.0%
	GAGTGTGCTTGAATTCCAAG+AGG	-	chr1.3:8696602-8696621	None:intergenic	45.0%
!	CCTCTTTATGGTGCTTTTGG+TGG	+	chr1.3:8696569-8696588	MS.gene33159:CDS	45.0%
!!	CATCAAGAGGAAGTGTTGGT+AGG	+	chr1.3:8696777-8696796	MS.gene33159:CDS	45.0%
!!	CCACCAAAAGCACCATAAAG+AGG	-	chr1.3:8696572-8696591	None:intergenic	45.0%
!	AAAGGTGTGCTTGGACTTGG+AGG	-	chr1.3:8696143-8696162	None:intergenic	50.0%
!	TGCAAAGGTGTGCTTGGACT+TGG	-	chr1.3:8696146-8696165	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	8696092	8696883	8696092	ID=MS.gene33159
chr1.3	mRNA	8696092	8696883	8696092	ID=MS.gene33159.t1;Parent=MS.gene33159
chr1.3	exon	8696092	8696883	8696092	ID=MS.gene33159.t1.exon1;Parent=MS.gene33159.t1
chr1.3	CDS	8696092	8696883	8696092	ID=cds.MS.gene33159.t1;Parent=MS.gene33159.t1

Gene Sequence

>MS.gene33159

ATGGAAAGACTAAGCAAGGAACCATGCATCTTCTCTTCTCAAGAAACTCCTCCAAGTCCAAGCACACCTTTGCAAAATCAACATGAAAAGAAGAAACAACAATCTATTGAGGTAGAAGAAGATATCACTAAGGTAGAAACTTTATTTGATCTAAATGTATCTAGCAATGATTCTAATCTTGATTGTAATAGTCAGCAAGAAACAGAACTCAATCTCATAACTTCTTTAGATGTTGGTAATTCATCATCACAAAACAATAATACTCTTGATGTAGAACAAAGAATTTTTTCTTGTAACTATTGTCATAGAAAATTCTATAGCTCACAAGCATTAGGTGGACACCAAAATGCTCACAAAAGAGAGAGATCAATAGCAAAAAGAGGACAAAGATTTGGAACACAAATAATAGCTTCAGCTTCTGCTTTTGGATTTCCTATTATGCATAATAAACAAAGTTATGCAAACATGGCTTCTTTGCCTCTTTATGGTGCTTTTGGTGGTAATAAACCTCTTGGAATTCAAGCACACTCTATGATCCATAAGCCTTCTCATGTTTCATCTAATGGATTTGGAAACTCTTATGGACATCATCATCATCATGGTTGGTCAAACTCAAGAATCTTTATGGATCAACAACCTGGAATAGCAAAACTAGGTATGGCTGATTTTCATAGAACAACAACATCATCAAGAGGAAGTGTTGGTAGGTTTGAAGTCGTAGATACTATGATTAATTCTGCAGCTAATAAAGAAATGAAGCAGAAGCAGCTTGATTTAAACCTCAAGCTATGA

Protein sequence

>MS.gene33159.t1

MERLSKEPCIFSSQETPPSPSTPLQNQHEKKKQQSIEVEEDITKVETLFDLNVSSNDSNLDCNSQQETELNLITSLDVGNSSSQNNNTLDVEQRIFSCNYCHRKFYSSQALGGHQNAHKRERSIAKRGQRFGTQIIASASAFGFPIMHNKQSYANMASLPLYGAFGGNKPLGIQAHSMIHKPSHVSSNGFGNSYGHHHHHGWSNSRIFMDQQPGIAKLGMADFHRTTTSSRGSVGRFEVVDTMINSAANKEMKQKQLDLNLKL