AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33202

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33202.t1 MTR_1g012520 97.356 643 17 0 1 643 1 643 0.0 1258
MS.gene33202.t1 MTR_3g107280 66.047 645 211 4 1 642 1 640 0.0 834
MS.gene33202.t1 MTR_4g114640 61.066 619 231 4 29 643 21 633 0.0 703
MS.gene33202.t1 MTR_4g114650 56.908 608 227 4 35 642 30 602 0.0 652
MS.gene33202.t1 MTR_4g114650 56.908 608 227 4 35 642 26 598 0.0 652
MS.gene33202.t1 MTR_4g114650 56.908 608 227 4 35 642 81 653 0.0 651
MS.gene33202.t1 MTR_2g021030 27.258 598 370 17 68 633 11 575 5.68e-39 152
MS.gene33202.t1 MTR_2g021030 27.295 414 261 11 145 558 205 578 3.99e-27 117
MS.gene33202.t1 MTR_2g021030 28.409 264 180 4 145 408 334 588 2.90e-24 108
MS.gene33202.t1 MTR_4g013255 27.243 602 361 24 37 606 28 584 2.69e-30 127
MS.gene33202.t1 MTR_4g013255 28.650 363 219 10 154 513 296 621 8.67e-28 119
MS.gene33202.t1 MTR_4g013255 26.048 334 213 11 321 628 77 402 7.10e-14 75.5
MS.gene33202.t1 MTR_7g112570 29.128 436 273 12 183 593 179 603 4.48e-29 122
MS.gene33202.t1 MTR_7g112570 26.898 461 274 14 205 611 92 543 1.20e-22 103
MS.gene33202.t1 MTR_7g112570 28.340 247 172 4 176 422 350 591 9.20e-19 90.9
MS.gene33202.t1 MTR_7g112570 24.706 340 212 9 176 484 273 599 2.08e-16 83.2
MS.gene33202.t1 MTR_7g112570 29.224 219 123 5 174 391 399 586 1.54e-15 80.5
MS.gene33202.t1 MTR_7g112570 29.621 449 278 14 183 606 179 614 5.82e-29 122
MS.gene33202.t1 MTR_7g112570 26.898 461 274 14 205 611 92 543 2.09e-22 102
MS.gene33202.t1 MTR_7g112570 28.340 247 172 4 176 422 350 591 1.31e-18 90.5
MS.gene33202.t1 MTR_7g112570 24.279 416 255 12 176 544 273 675 2.87e-17 86.3
MS.gene33202.t1 MTR_7g112570 27.273 264 179 6 174 433 399 653 5.46e-17 85.5
MS.gene33202.t1 MTR_7g112570 29.167 432 270 12 183 589 179 599 6.77e-29 122
MS.gene33202.t1 MTR_7g112570 26.898 461 274 14 205 611 92 543 1.75e-22 102
MS.gene33202.t1 MTR_7g112570 28.340 247 172 4 176 422 350 591 9.30e-19 90.9
MS.gene33202.t1 MTR_7g112570 24.706 340 212 9 176 484 273 599 2.21e-16 83.2
MS.gene33202.t1 MTR_7g112570 29.224 219 123 5 174 391 399 586 1.92e-15 80.1
MS.gene33202.t1 MTR_7g112570 29.688 128 87 2 176 301 478 604 7.38e-11 65.5
MS.gene33202.t1 MTR_2g021030 30.450 289 193 7 176 463 153 434 6.08e-27 114
MS.gene33202.t1 MTR_2g021030 28.859 298 156 7 228 500 127 393 2.40e-21 97.8
MS.gene33202.t1 MTR_2g021030 34.028 144 89 1 145 288 282 419 2.10e-14 76.3
MS.gene33202.t1 MTR_2g021030 26.316 247 150 5 386 632 127 341 3.47e-12 69.3
MS.gene33202.t1 MTR_1g114610 26.913 379 255 8 66 436 18 382 3.37e-26 112
MS.gene33202.t1 MTR_1g114610 28.968 252 171 4 142 391 145 390 2.85e-19 91.3
MS.gene33202.t1 MTR_1g114610 25.625 320 206 8 252 569 93 382 2.93e-19 91.3
MS.gene33202.t1 MTR_1g114610 25.143 350 195 7 202 518 95 410 7.46e-19 89.7
MS.gene33202.t1 MTR_1g114610 29.609 179 123 3 455 632 110 286 5.66e-14 74.7
MS.gene33202.t1 MTR_8g028140 27.704 379 243 7 53 417 7 368 1.22e-23 103
MS.gene33202.t1 MTR_8g028140 27.667 300 186 8 360 635 82 374 5.24e-16 80.9
MS.gene33202.t1 MTR_8g028140 29.851 268 166 3 161 417 32 288 4.23e-21 95.1
MS.gene33202.t1 MTR_8g028140 27.972 286 175 8 374 635 16 294 4.30e-16 80.1
MS.gene33202.t1 MTR_3g088895 29.474 190 107 2 210 399 123 285 7.32e-19 89.4
MS.gene33202.t1 MTR_3g088895 26.341 205 133 4 176 377 141 330 8.08e-13 70.9
MS.gene33202.t1 MTR_1g015480 25.424 413 238 15 188 582 157 517 4.36e-18 89.0
MS.gene33202.t1 MTR_1g029500 26.577 222 124 3 204 413 104 298 6.44e-18 86.3
MS.gene33202.t1 MTR_1g029500 27.751 209 140 2 180 377 106 314 6.43e-14 73.9
MS.gene33202.t1 MTR_1g029500 26.866 201 108 3 143 307 127 324 3.03e-11 65.9
MS.gene33202.t1 MTR_4g019320 21.262 428 292 10 166 569 109 515 1.08e-17 87.4
MS.gene33202.t1 MTR_1g015450 25.666 413 237 15 188 582 339 699 1.82e-17 87.4
MS.gene33202.t1 MTR_1g015450 25.042 595 270 22 180 630 377 939 2.72e-13 73.9
MS.gene33202.t1 MTR_4g009030 23.877 423 280 10 200 609 154 547 8.32e-16 81.3
MS.gene33202.t1 MTR_1g054625 22.830 311 176 5 228 533 92 343 1.95e-15 78.6
MS.gene33202.t1 MTR_1g054625 27.907 301 196 12 336 634 58 339 1.72e-14 75.9
MS.gene33202.t1 MTR_1g054625 25.820 244 169 7 319 558 79 314 2.95e-14 75.1
MS.gene33202.t1 MTR_1g054625 28.713 202 138 5 174 374 120 316 1.07e-13 73.6
MS.gene33202.t1 MTR_1g054625 22.426 272 177 4 190 457 110 351 7.14e-12 67.8
MS.gene33202.t1 MTR_1g054625 22.526 293 167 4 242 533 13 246 4.14e-14 73.2
MS.gene33202.t1 MTR_1g054625 28.780 205 140 5 171 374 20 219 6.32e-14 72.8
MS.gene33202.t1 MTR_5g075860 22.414 406 265 9 216 609 175 542 1.28e-13 74.3
MS.gene33202.t1 MTR_4g036815 25.843 356 200 13 202 555 189 482 1.74e-13 73.6
MS.gene33202.t1 MTR_4g094932 24.508 457 279 16 66 506 44 450 3.37e-12 69.7
MS.gene33202.t1 MTR_1g018790 23.729 295 199 9 201 484 137 416 1.19e-11 68.2
MS.gene33202.t1 MTR_5g095830 21.868 471 299 15 176 609 145 583 1.39e-11 68.2
MS.gene33202.t1 MTR_4g130620 30.168 179 109 6 229 405 102 266 1.96e-11 65.9
MS.gene33202.t1 MTR_1g114010 23.950 238 149 4 174 409 136 343 2.85e-11 66.6
MS.gene33202.t1 MTR_1g114010 23.950 238 149 4 174 409 145 352 3.01e-11 66.6
MS.gene33202.t1 MTR_1g114010 23.950 238 149 4 174 409 215 422 3.05e-11 66.6
MS.gene33202.t1 MTR_1g114010 24.066 241 151 4 174 412 296 506 4.35e-11 66.2
MS.gene33202.t1 MTR_4g125410 25.993 277 165 9 358 600 143 413 4.92e-11 65.9
MS.gene33202.t1 MTR_3g006050 22.286 350 218 10 202 541 192 497 7.50e-11 65.5
MS.gene33202.t1 MTR_4g125440 24.731 279 196 8 346 618 397 667 8.63e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33202.t1 AT2G25490 60.836 646 232 8 1 643 1 628 0.0 768
MS.gene33202.t1 AT5G25350 58.385 644 244 8 1 642 1 622 0.0 694
MS.gene33202.t1 AT4G15475 30.562 445 293 13 200 642 125 555 1.80e-43 165
MS.gene33202.t1 AT4G15475 27.228 606 360 16 68 633 11 575 5.47e-39 152
MS.gene33202.t1 AT4G15475 26.471 340 241 4 125 460 252 586 1.77e-30 127
MS.gene33202.t1 AT4G15475 25.907 386 246 10 145 503 205 577 2.28e-25 111
MS.gene33202.t1 AT5G27920 26.177 531 331 16 152 631 82 602 1.96e-29 124
MS.gene33202.t1 AT5G27920 24.270 445 278 14 217 609 67 504 1.51e-19 93.6
MS.gene33202.t1 AT5G23340 26.780 295 185 4 306 599 85 349 3.70e-25 108
MS.gene33202.t1 AT5G23340 24.725 364 262 7 66 426 8 362 1.68e-22 100
MS.gene33202.t1 AT5G01720 27.308 509 287 15 176 610 85 584 1.11e-23 106
MS.gene33202.t1 AT5G01720 26.882 372 207 10 129 500 274 580 1.63e-20 96.7
MS.gene33202.t1 AT5G01720 26.923 208 117 5 176 381 444 618 3.27e-14 76.6
MS.gene33202.t1 AT5G01720 27.301 326 227 9 307 632 61 376 1.32e-12 71.2
MS.gene33202.t1 AT1G21410 24.390 328 164 5 204 531 106 349 6.11e-20 92.4
MS.gene33202.t1 AT1G21410 30.636 173 115 5 464 634 102 271 1.86e-13 72.8
MS.gene33202.t1 AT1G21410 23.256 215 148 2 180 377 108 322 1.71e-12 69.7
MS.gene33202.t1 AT1G21410 24.390 328 164 5 204 531 106 349 6.11e-20 92.4
MS.gene33202.t1 AT1G21410 30.636 173 115 5 464 634 102 271 1.86e-13 72.8
MS.gene33202.t1 AT1G21410 23.256 215 148 2 180 377 108 322 1.71e-12 69.7
MS.gene33202.t1 AT1G77000 29.082 196 112 2 204 399 106 274 2.10e-18 87.8
MS.gene33202.t1 AT1G77000 25.641 234 140 4 180 379 108 341 3.24e-14 75.1
MS.gene33202.t1 AT1G77000 29.082 196 112 2 204 399 106 274 2.10e-18 87.8
MS.gene33202.t1 AT1G77000 25.641 234 140 4 180 379 108 341 3.24e-14 75.1
MS.gene33202.t1 AT4G33210 23.830 470 265 16 131 570 289 695 8.73e-17 85.1
MS.gene33202.t1 AT3G58530 27.090 299 177 10 347 638 81 345 1.03e-14 76.6
MS.gene33202.t1 AT3G58530 24.715 263 156 9 180 439 128 351 1.55e-14 75.9
MS.gene33202.t1 AT3G58530 21.283 343 207 6 193 530 58 342 1.66e-14 75.9
MS.gene33202.t1 AT1G80630 23.383 402 235 9 200 569 148 508 5.94e-14 75.5
MS.gene33202.t1 AT2G06040 24.911 281 200 6 337 610 473 749 1.60e-13 74.3
MS.gene33202.t1 AT1G55590 24.889 225 164 4 172 394 294 515 1.92e-13 73.9
MS.gene33202.t1 AT3G50080 24.222 450 259 16 57 500 33 406 1.96e-12 70.5
MS.gene33202.t1 AT1G47056 23.913 460 294 16 41 492 15 426 2.31e-12 70.5
MS.gene33202.t1 AT1G47056 28.082 292 142 8 347 581 130 410 3.06e-11 66.6
MS.gene33202.t1 AT4G07400 30.667 150 76 3 177 299 330 478 3.07e-12 70.1
MS.gene33202.t1 AT1G77000 26.087 184 83 3 204 387 106 236 3.54e-12 67.4
MS.gene33202.t1 AT1G77000 26.087 184 83 3 204 387 106 236 3.54e-12 67.4
MS.gene33202.t1 AT3G07550 24.367 316 196 8 193 500 62 342 4.08e-12 68.9
MS.gene33202.t1 AT3G07550 28.094 299 169 9 348 641 118 375 1.36e-11 67.4
MS.gene33202.t1 AT3G07550 24.367 316 196 8 193 500 62 342 4.08e-12 68.9
MS.gene33202.t1 AT3G07550 28.094 299 169 9 348 641 118 375 1.36e-11 67.4
MS.gene33202.t1 AT3G07550 28.094 299 169 9 348 641 91 348 7.24e-12 67.8
MS.gene33202.t1 AT3G07550 24.042 287 182 7 215 500 64 315 8.50e-12 67.8

Find 160 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAAGTCTTTCTCTTTA+TGG 0.012962 1.2:-9109500 MS.gene33202:CDS
TGCCACAGAATTACCCAATT+TGG 0.171018 1.2:-9108844 MS.gene33202:CDS
CTTACCCGCTTTGAGAAAAT+TGG 0.171059 1.2:-9108263 MS.gene33202:CDS
GATCTCTCTCTAGCTGTTAT+TGG 0.220084 1.2:-9109147 MS.gene33202:CDS
TAACATTTGGGAGGAAATTA+AGG 0.226363 1.2:+9109094 None:intergenic
ATAGATATAACCTTAAGATT+TGG 0.265371 1.2:+9109270 None:intergenic
CCTTCTTTCCTTCCAAGCTT+CGG 0.280846 1.2:+9109661 None:intergenic
TGAGAGAGGCTTCTGGGTTA+TGG 0.281774 1.2:-9109070 MS.gene33202:CDS
ATCTGTAACTGCCCTGGATT+TGG 0.285015 1.2:-9108691 MS.gene33202:CDS
CAGGAAGAAATTTGCTTCCT+TGG 0.285276 1.2:+9110007 None:intergenic
TTGTGTGCGATCTCCGATTC+CGG 0.288491 1.2:-9108367 MS.gene33202:CDS
CTTCTTTCCTTCCAAGCTTC+GGG 0.291790 1.2:+9109662 None:intergenic
CTTCATCAAGTTGAATTAAC+TGG 0.293196 1.2:-9108628 MS.gene33202:CDS
CTCAAATGACGAGAATAAAA+TGG 0.294170 1.2:-9109724 MS.gene33202:CDS
ACTGCATCACGAGGAGGATT+AGG 0.301726 1.2:-9109603 MS.gene33202:CDS
AAAATTGGGCCACACCCTTT+TGG 0.309297 1.2:-9108248 MS.gene33202:CDS
TTTCTATGGTAAGCTGCTTT+GGG 0.317243 1.2:-9108777 MS.gene33202:CDS
TGTGGCACTCTTCCAATTGT+AGG 0.329715 1.2:+9108860 None:intergenic
TCAAGGACTGTGCCGGTGTT+GGG 0.330559 1.2:-9109248 MS.gene33202:CDS
GTTGAATTAACTGGACTTAA+GGG 0.337021 1.2:-9108619 MS.gene33202:CDS
AGATGCTTGTTGAACATCTT+TGG 0.338078 1.2:-9108180 MS.gene33202:CDS
TAGATATAACCTTAAGATTT+GGG 0.344478 1.2:+9109271 None:intergenic
GCCACAGAATTACCCAATTT+GGG 0.348800 1.2:-9108843 MS.gene33202:CDS
AGTTGAATTAACTGGACTTA+AGG 0.350149 1.2:-9108620 MS.gene33202:CDS
AGGAAGAAATTTGCTTCCTT+GGG 0.350453 1.2:+9110008 None:intergenic
GCAAATTTCTTCCTGTCTCT+TGG 0.351075 1.2:-9109999 MS.gene33202:CDS
CTCGAAATTCTGAACCTTGA+AGG 0.357149 1.2:-9108466 MS.gene33202:CDS
ATGTTAGTGAGAGAGGCTTC+TGG 0.357800 1.2:-9109077 MS.gene33202:CDS
AAGCCTCTCTCACTAACATT+TGG 0.362156 1.2:+9109081 None:intergenic
GTTTCTTCTGTTGGTGATGA+AGG 0.367259 1.2:-9109474 MS.gene33202:CDS
TTATGGAATGTTTCTTCTGT+TGG 0.375011 1.2:-9109483 MS.gene33202:CDS
GAGAGAGGCTTCTGGGTTAT+GGG 0.375727 1.2:-9109069 MS.gene33202:CDS
GCCTCTTTGAGATCTTTAGA+AGG 0.392355 1.2:-9109854 MS.gene33202:CDS
TGGGTTATGGGAAATGCTAA+TGG 0.394159 1.2:-9109057 MS.gene33202:CDS
GTCAGGCTGCACTTTGGTTA+CGG 0.399322 1.2:-9108293 MS.gene33202:CDS
CCCTGGTCAATCTGCATGGT+TGG 0.402559 1.2:-9108492 MS.gene33202:CDS
ATCCATTAGCGATCTCAATA+AGG 0.403036 1.2:+9109451 None:intergenic
GAAAGTGACTGTGAAGAGTT+TGG 0.407597 1.2:-9109702 MS.gene33202:CDS
TGTGTGCGATCTCCGATTCC+GGG 0.408544 1.2:-9108366 MS.gene33202:CDS
ATCACTTGAAAGCCTACAAT+TGG 0.412655 1.2:-9108872 MS.gene33202:CDS
CCAAAGAAGTCGAAGAAAAC+AGG 0.414246 1.2:+9109208 None:intergenic
CTGCATCACGAGGAGGATTA+GGG 0.415640 1.2:-9109602 MS.gene33202:CDS
AGCCTCTCTCACTAACATTT+GGG 0.422860 1.2:+9109082 None:intergenic
GTGTGCCAGGGCTGCTATCC+CGG 0.423492 1.2:+9108348 None:intergenic
CTTAAGGGAGTCACAGATGC+TGG 0.425211 1.2:-9108604 MS.gene33202:CDS
GGCCTTATTGAGATCGCTAA+TGG 0.425507 1.2:-9109453 MS.gene33202:CDS
CATCAAGATCACAAAGCAAT+TGG 0.425806 1.2:+9108395 None:intergenic
ATCTCAAAGAGGCACTCATC+TGG 0.426649 1.2:+9109864 None:intergenic
AAAGTTCTGCCCAAATCTTA+AGG 0.427807 1.2:-9109280 MS.gene33202:CDS
CTACAGATTCTTTCTCTGTC+AGG 0.430438 1.2:-9108310 MS.gene33202:CDS
CGATTCCGGGATAGCAGCCC+TGG 0.432829 1.2:-9108353 MS.gene33202:CDS
TGTTAGTGAGAGAGGCTTCT+GGG 0.434709 1.2:-9109076 MS.gene33202:CDS
AGGGTAGCGTTGCCAAATCC+AGG 0.436782 1.2:+9108679 None:intergenic
CATTCTCCATCGAAAACAAA+TGG 0.441047 1.2:+9109924 None:intergenic
AACTGCGATTAAAGCCTTGT+CGG 0.441499 1.2:+9109383 None:intergenic
TTACCCGCTTTGAGAAAATT+GGG 0.441746 1.2:-9108262 MS.gene33202:CDS
AAAGCCTTGTCGGAAATTGC+AGG 0.442003 1.2:+9109393 None:intergenic
TAACTGTGGTGCAAAATTGA+AGG 0.443191 1.2:-9108803 MS.gene33202:CDS
CAGAAGCATAAGCCAGCGTT+TGG 0.444467 1.2:+9109791 None:intergenic
CTGCTTTGGGATCAAGGATC+TGG 0.448619 1.2:-9108764 MS.gene33202:CDS
GCACTCCTTTCTTCACCAAC+AGG 0.449873 1.2:+9109828 None:intergenic
GGTAAGCTGCTTTGGGATCA+AGG 0.450353 1.2:-9108770 MS.gene33202:CDS
GCTGGATATTTAGTCCCAAA+AGG 0.450825 1.2:+9108233 None:intergenic
ATCAAGGACTGTGCCGGTGT+TGG 0.452196 1.2:-9109249 MS.gene33202:CDS
AACTTTAACAAGACCAGCCT+CGG 0.457238 1.2:+9108558 None:intergenic
CTAGCTGTTATTGGACACTA+TGG 0.460329 1.2:-9109138 MS.gene33202:CDS
TTCAAGTGATATAGCAGCCT+TGG 0.460685 1.2:+9108885 None:intergenic
GGGATACCTTTCCCGAAGCT+TGG 0.461300 1.2:-9109673 MS.gene33202:CDS
CAACTTGAGGGCCAAGAGAC+AGG 0.463141 1.2:+9109988 None:intergenic
TGTGCCGGTGTTGGGGATCA+AGG 0.466975 1.2:-9109240 MS.gene33202:CDS
TTATCGATCTGTAACTGCCC+TGG 0.467205 1.2:-9108697 MS.gene33202:CDS
CCGAAGCTTGGAAGGAAAGA+AGG 0.469354 1.2:-9109661 MS.gene33202:CDS
CTCCCAAATGTTAGTGAGAG+AGG 0.473423 1.2:-9109084 MS.gene33202:CDS
CGCGTTCTGAGTTGCTTCCA+CGG 0.473495 1.2:+9109568 None:intergenic
TTGCTTGAGAGCTCCGAGGC+TGG 0.476623 1.2:-9108571 MS.gene33202:CDS
CTGCTTGTGTTTCCAAACGC+TGG 0.478903 1.2:-9109803 MS.gene33202:CDS
TGGGATCAAGGATCTGGACT+TGG 0.479690 1.2:-9108758 MS.gene33202:CDS
AGACTTGCTGCTATTGCAGT+TGG 0.480536 1.2:-9109627 MS.gene33202:CDS
CAGGGCTGCTATCCCGGAAT+CGG 0.488751 1.2:+9108354 None:intergenic
GCAGAGGAGTAACCGATGTT+GGG 0.492742 1.2:-9108999 MS.gene33202:CDS
AACGCTACCCTTTCTGTACT+CGG 0.496998 1.2:-9108667 MS.gene33202:CDS
TTCTCTGTCAGGCTGCACTT+TGG 0.500531 1.2:-9108299 MS.gene33202:CDS
GCACCCATGTCTCAAGTCTT+CGG 0.505219 1.2:-9110665 None:intergenic
CAGGGATGAAACTACTTTGT+CGG 0.506121 1.2:+9108510 None:intergenic
TCTTAAGGTTATATCTATCA+AGG 0.507799 1.2:-9109265 MS.gene33202:CDS
TGCAGAGGAGTAACCGATGT+TGG 0.509560 1.2:-9109000 MS.gene33202:CDS
GCCTCGGAGCTCTCAAGCAA+TGG 0.512849 1.2:+9108574 None:intergenic
AGTGATTCACAATGAGATAC+AGG 0.513908 1.2:+9108727 None:intergenic
GGTCTCAAAGCAGTTGCTTG+TGG 0.514047 1.2:-9109531 MS.gene33202:CDS
GGTTGCCTGTTGGTGAAGAA+AGG 0.514240 1.2:-9109833 MS.gene33202:CDS
GACTTCTTTGGTTCTGACAA+AGG 0.516837 1.2:-9109196 MS.gene33202:CDS
TGCTGATTGAATTGCAATGC+TGG 0.518629 1.2:+9108215 None:intergenic
AGACCTTCATTGCGAATGCT+AGG 0.519816 1.2:+9109315 None:intergenic
TGTCCTAGCATTCGCAATGA+AGG 0.520385 1.2:-9109318 MS.gene33202:CDS
GAACGCGGCGTGACAACTCT+AGG 0.525236 1.2:-9109552 MS.gene33202:CDS
TACCTTTCCCGAAGCTTGGA+AGG 0.529475 1.2:-9109669 MS.gene33202:CDS
CAAGGACTGTGCCGGTGTTG+GGG 0.530233 1.2:-9109247 MS.gene33202:CDS
TGCTTGTTGAACATCTTTGG+AGG 0.531336 1.2:-9108177 MS.gene33202:CDS
TTCCTTCCAAGCTTCGGGAA+AGG 0.533717 1.2:+9109667 None:intergenic
CTGGATATTTAGTCCCAAAA+GGG 0.534654 1.2:+9108234 None:intergenic
TCCAACCATGCAGATTGACC+AGG 0.536554 1.2:+9108491 None:intergenic
AACATCGGTTACTCCTCTGC+AGG 0.539479 1.2:+9109002 None:intergenic
AAAGGTGAAGCTTCAAGCAC+TGG 0.541994 1.2:-9109178 MS.gene33202:CDS
AGCTAGAGAGAGATCAGAAA+CGG 0.542100 1.2:+9109155 None:intergenic
GGGATTGAAGCTGTTGGAAA+GGG 0.549164 1.2:-9108979 MS.gene33202:CDS
TCATCCCTGGTCAATCTGCA+TGG 0.552095 1.2:-9108496 MS.gene33202:CDS
GTTGGGGATCAAGGAATCGC+TGG 0.553667 1.2:-9109231 MS.gene33202:CDS
TAAATCTTGCAGTTCAGGAA+AGG 0.554597 1.2:+9108146 None:intergenic
CTGTGAAGAGTTTGGAGGTG+AGG 0.561043 1.2:-9109694 MS.gene33202:CDS
GATAGTAGACATCAACTTGA+GGG 0.562773 1.2:+9109976 None:intergenic
CGACAAAGTAGTTTCATCCC+TGG 0.562846 1.2:-9108509 MS.gene33202:CDS
TGTGAAGAGTTTGGAGGTGA+GGG 0.567927 1.2:-9109693 MS.gene33202:CDS
TTAGGGAAGCTTTCAATCCG+TGG 0.570647 1.2:-9109585 MS.gene33202:CDS
GCTGCACTTTGGTTACGGAC+AGG 0.573743 1.2:-9108288 MS.gene33202:CDS
ATATCTATCAAGGACTGTGC+CGG 0.574521 1.2:-9109255 MS.gene33202:CDS
ACAACTTTCCGAGTACAGAA+AGG 0.575435 1.2:+9108659 None:intergenic
AAAGAGAAAGACTTTAGAGA+AGG 0.577504 1.2:+9109504 None:intergenic
CAACTTTCCGAGTACAGAAA+GGG 0.577567 1.2:+9108660 None:intergenic
TTAAGGGAGTCACAGATGCT+GGG 0.582917 1.2:-9108603 MS.gene33202:CDS
AAATTGCAGGGCATTTGCAA+AGG 0.585154 1.2:+9109406 None:intergenic
CTTGTTAAAGTTAATCTCAG+TGG 0.588339 1.2:-9108547 MS.gene33202:CDS
CGGATTGATTTCATTCACCA+AGG 0.590158 1.2:-9108902 MS.gene33202:CDS
AATGAAGGTCTACAAGCCAT+TGG 0.590793 1.2:-9109303 MS.gene33202:CDS
TTTATTCTCGTCATTTGAGG+AGG 0.593111 1.2:+9109728 None:intergenic
AAGCCTTGTCGGAAATTGCA+GGG 0.597068 1.2:+9109394 None:intergenic
ATTTGGGCAGAACTTTCCAA+TGG 0.599132 1.2:+9109287 None:intergenic
AATTCAACTTGATGAAGCTG+AGG 0.602123 1.2:+9108634 None:intergenic
TATTTAGTCCCAAAAGGGTG+TGG 0.603959 1.2:+9108239 None:intergenic
GAGGAAATTAAGGACAAGAT+CGG 0.606521 1.2:+9109104 None:intergenic
TGTGCATTTCTCTCAGACAA+CGG 0.609269 1.2:-9108922 MS.gene33202:CDS
GGAAGAAATTTGCTTCCTTG+GGG 0.618210 1.2:+9110009 None:intergenic
TGGGATTGAAGCTGTTGGAA+AGG 0.620246 1.2:-9108980 MS.gene33202:CDS
CTCACAATTGCATCCTGCAG+AGG 0.621245 1.2:-9109015 MS.gene33202:CDS
AACAGCTTCAATCCCAACAT+CGG 0.622290 1.2:+9108987 None:intergenic
AATGATAACTCGGTCAGAGT+CGG 0.624354 1.2:+9109348 None:intergenic
ACCCAAATTGGGTAATTCTG+TGG 0.625237 1.2:+9108842 None:intergenic
ATCTTTAGAAGGTTGCCTGT+TGG 0.625556 1.2:-9109843 MS.gene33202:CDS
GGATAGTAGACATCAACTTG+AGG 0.626268 1.2:+9109975 None:intergenic
ACACGTGATCTCTTCTGAGG+TGG 0.627046 1.2:+9109954 None:intergenic
ATGCCCTGCAATTTCCGACA+AGG 0.628954 1.2:-9109397 MS.gene33202:CDS
GGAATCGGAGATCGCACACA+AGG 0.629259 1.2:+9108369 None:intergenic
GGGTAGCGTTGCCAAATCCA+GGG 0.632564 1.2:+9108680 None:intergenic
CTGACACGTGATCTCTTCTG+AGG 0.633789 1.2:+9109951 None:intergenic
ACAAGACTCTAATGATAACT+CGG 0.633790 1.2:+9109338 None:intergenic
GATGTTGGGATTGAAGCTGT+TGG 0.634700 1.2:-9108985 MS.gene33202:CDS
CTCTCTCACTAACATTTGGG+AGG 0.636568 1.2:+9109085 None:intergenic
AGTGACTGTGAAGAGTTTGG+AGG 0.636749 1.2:-9109699 MS.gene33202:CDS
AAAGCCGAAGACTTGAGACA+TGG 0.637618 1.2:+9110661 None:intergenic
ATTCAACTTGATGAAGCTGA+GGG 0.638327 1.2:+9108635 None:intergenic
AAGCCGAAGACTTGAGACAT+GGG 0.649190 1.2:+9110662 None:intergenic
GATTCCTTGATCCCCAACAC+CGG 0.655421 1.2:+9109236 None:intergenic
TCCATTGCTTGAGAGCTCCG+AGG 0.656114 1.2:-9108575 MS.gene33202:CDS
ACCTTCTAAAGATCTCAAAG+AGG 0.665153 1.2:+9109853 None:intergenic
CGTGGAAGCAACTCAGAACG+CGG 0.669342 1.2:-9109567 MS.gene33202:CDS
GTTGGAACTGCATCACGAGG+AGG 0.679979 1.2:-9109609 MS.gene33202:CDS
CCAACCATGCAGATTGACCA+GGG 0.686419 1.2:+9108492 None:intergenic
GGTTTGTGTATAACCCACCA+TGG 0.686541 1.2:+9110030 None:intergenic
AATAGCAGCAAGTCTAACAT+CGG 0.688914 1.2:+9109635 None:intergenic
GCAGTTGGAACTGCATCACG+AGG 0.700255 1.2:-9109612 MS.gene33202:CDS
TGGGTTATACACAAACCCCA+AGG 0.726645 1.2:-9110024 MS.gene33202:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTGATTCATAGATTTTT+AGG - chr1.2:9108211-9108230 MS.gene33202:CDS 15.0%
!! AATTCTCTTAATATGTTATG+TGG - chr1.2:9108385-9108404 MS.gene33202:CDS 20.0%
!! ATAGATATAACCTTAAGATT+TGG + chr1.2:9109551-9109570 None:intergenic 20.0%
!! GAATCAAATAAAAAACGTAA+AGG + chr1.2:9108201-9108220 None:intergenic 20.0%
!! TAGATATAACCTTAAGATTT+GGG + chr1.2:9109550-9109569 None:intergenic 20.0%
!!! TTTTACTTTTCTGATATCAA+AGG - chr1.2:9108600-9108619 MS.gene33202:CDS 20.0%
! AGATCTATGAGATTAGTATA+TGG - chr1.2:9108467-9108486 MS.gene33202:CDS 25.0%
! GATCTATGAGATTAGTATAT+GGG - chr1.2:9108468-9108487 MS.gene33202:CDS 25.0%
! TCTTAAGGTTATATCTATCA+AGG - chr1.2:9109553-9109572 MS.gene33202:CDS 25.0%
! TCTTAATATGTTATGTGGTA+TGG - chr1.2:9108390-9108409 MS.gene33202:CDS 25.0%
!!! AAAATTGAAGGCTTTTTCTA+TGG - chr1.2:9110027-9110046 MS.gene33202:CDS 25.0%
!!! ATGATGATTTTGATGTTCAT+AGG - chr1.2:9108298-9108317 MS.gene33202:CDS 25.0%
!!! ATTCATAGATTTTTAGGTTG+TGG - chr1.2:9108217-9108236 MS.gene33202:CDS 25.0%
!!! TGATATTTTTACAACCTTCA+AGG + chr1.2:9110369-9110388 None:intergenic 25.0%
!!! TTCATAGATTTTTAGGTTGT+GGG - chr1.2:9108218-9108237 MS.gene33202:CDS 25.0%
ACAAGACTCTAATGATAACT+CGG + chr1.2:9109483-9109502 None:intergenic 30.0%
AGTTGAATTAACTGGACTTA+AGG - chr1.2:9110198-9110217 MS.gene33202:intron 30.0%
CTCAAATGACGAGAATAAAA+TGG - chr1.2:9109094-9109113 MS.gene33202:CDS 30.0%
CTTGTTAAAGTTAATCTCAG+TGG - chr1.2:9110271-9110290 MS.gene33202:intron 30.0%
GTTGAATTAACTGGACTTAA+GGG - chr1.2:9110199-9110218 MS.gene33202:intron 30.0%
TAACATTTGGGAGGAAATTA+AGG + chr1.2:9109727-9109746 None:intergenic 30.0%
TAAGAGGTGAAATTTCAACT+GGG - chr1.2:9108426-9108445 MS.gene33202:CDS 30.0%
TATGAGATTAGTATATGGGA+GGG - chr1.2:9108472-9108491 MS.gene33202:CDS 30.0%
TTAAGAGGTGAAATTTCAAC+TGG - chr1.2:9108425-9108444 MS.gene33202:CDS 30.0%
TTATGGAATGTTTCTTCTGT+TGG - chr1.2:9109335-9109354 MS.gene33202:CDS 30.0%
! AAAGAGAAAGACTTTAGAGA+AGG + chr1.2:9109317-9109336 None:intergenic 30.0%
! AGATGTACGCTTTTCAAATT+AGG + chr1.2:9109869-9109888 None:intergenic 30.0%
! ATTTTGCACCACAGTTAAAA+AGG + chr1.2:9110012-9110031 None:intergenic 30.0%
! CATTTTATTCTCGTCATTTG+AGG + chr1.2:9109096-9109115 None:intergenic 30.0%
!! ATTACCCAATTTGGGTTTTT+CGG - chr1.2:9109983-9110002 MS.gene33202:CDS 30.0%
!! CTCTAAAGTCTTTCTCTTTA+TGG - chr1.2:9109318-9109337 MS.gene33202:CDS 30.0%
!! CTTCATCAAGTTGAATTAAC+TGG - chr1.2:9110190-9110209 MS.gene33202:intron 30.0%
!!! AGCAGTTTTGTTGAATTTGA+TGG - chr1.2:9108663-9108682 MS.gene33202:CDS 30.0%
AAAGTTCTGCCCAAATCTTA+AGG - chr1.2:9109538-9109557 MS.gene33202:CDS 35.0%
AACGATTGTGCAAAATCATC+AGG + chr1.2:9108744-9108763 None:intergenic 35.0%
AATAGCAGCAAGTCTAACAT+CGG + chr1.2:9109186-9109205 None:intergenic 35.0%
AATTCAACTTGATGAAGCTG+AGG + chr1.2:9110187-9110206 None:intergenic 35.0%
AGTGATTCACAATGAGATAC+AGG + chr1.2:9110094-9110113 None:intergenic 35.0%
ATCACTTGAAAGCCTACAAT+TGG - chr1.2:9109946-9109965 MS.gene33202:CDS 35.0%
ATCCATTAGCGATCTCAATA+AGG + chr1.2:9109370-9109389 None:intergenic 35.0%
ATGTGGTATGGATAATCATG+TGG - chr1.2:9108402-9108421 MS.gene33202:CDS 35.0%
ATTCAACTTGATGAAGCTGA+GGG + chr1.2:9110186-9110205 None:intergenic 35.0%
CAACCAAAAATAGTAGCATG+TGG - chr1.2:9108257-9108276 MS.gene33202:CDS 35.0%
CATCAAGATCACAAAGCAAT+TGG + chr1.2:9110426-9110445 None:intergenic 35.0%
CATTCTCCATCGAAAACAAA+TGG + chr1.2:9108897-9108916 None:intergenic 35.0%
CTATGAGATTAGTATATGGG+AGG - chr1.2:9108471-9108490 MS.gene33202:CDS 35.0%
CTGGATATTTAGTCCCAAAA+GGG + chr1.2:9110587-9110606 None:intergenic 35.0%
GAGGAAATTAAGGACAAGAT+CGG + chr1.2:9109717-9109736 None:intergenic 35.0%
GATAGTAGACATCAACTTGA+GGG + chr1.2:9108845-9108864 None:intergenic 35.0%
GATGAGATAGTGAGATACTT+TGG - chr1.2:9108559-9108578 MS.gene33202:CDS 35.0%
TAACTGTGGTGCAAAATTGA+AGG - chr1.2:9110015-9110034 MS.gene33202:CDS 35.0%
TGGATAATCATGTGGTTAAG+AGG - chr1.2:9108410-9108429 MS.gene33202:CDS 35.0%
TTTATTCTCGTCATTTGAGG+AGG + chr1.2:9109093-9109112 None:intergenic 35.0%
! ACCTTCTAAAGATCTCAAAG+AGG + chr1.2:9108968-9108987 None:intergenic 35.0%
! AGATGCTTGTTGAACATCTT+TGG - chr1.2:9110638-9110657 MS.gene33202:intron 35.0%
! AGGAAGAAATTTGCTTCCTT+GGG + chr1.2:9108813-9108832 None:intergenic 35.0%
! GAACCACATGCTACTATTTT+TGG + chr1.2:9108263-9108282 None:intergenic 35.0%
! TTTCTATGGTAAGCTGCTTT+GGG - chr1.2:9110041-9110060 MS.gene33202:CDS 35.0%
! TTTTCTATGGTAAGCTGCTT+TGG - chr1.2:9110040-9110059 MS.gene33202:CDS 35.0%
!! AGTGTTCCATTTGTTTTCGA+TGG - chr1.2:9108888-9108907 MS.gene33202:CDS 35.0%
!! CATTTGTTTTCGATGGAGAA+TGG - chr1.2:9108895-9108914 MS.gene33202:CDS 35.0%
!! TTACCCGCTTTGAGAAAATT+GGG - chr1.2:9110556-9110575 MS.gene33202:intron 35.0%
AACAGCTTCAATCCCAACAT+CGG + chr1.2:9109834-9109853 None:intergenic 40.0%
AACTTTAACAAGACCAGCCT+CGG + chr1.2:9110263-9110282 None:intergenic 40.0%
AAGCCTCTCTCACTAACATT+TGG + chr1.2:9109740-9109759 None:intergenic 40.0%
AATGAAGGTCTACAAGCCAT+TGG - chr1.2:9109515-9109534 MS.gene33202:CDS 40.0%
AATGATAACTCGGTCAGAGT+CGG + chr1.2:9109473-9109492 None:intergenic 40.0%
ACAACTTTCCGAGTACAGAA+AGG + chr1.2:9110162-9110181 None:intergenic 40.0%
ACCCAAATTGGGTAATTCTG+TGG + chr1.2:9109979-9109998 None:intergenic 40.0%
AGCCTCTCTCACTAACATTT+GGG + chr1.2:9109739-9109758 None:intergenic 40.0%
AGCTAGAGAGAGATCAGAAA+CGG + chr1.2:9109666-9109685 None:intergenic 40.0%
ATATCTATCAAGGACTGTGC+CGG - chr1.2:9109563-9109582 MS.gene33202:CDS 40.0%
ATTTGGGCAGAACTTTCCAA+TGG + chr1.2:9109534-9109553 None:intergenic 40.0%
CAACTTTCCGAGTACAGAAA+GGG + chr1.2:9110161-9110180 None:intergenic 40.0%
CAGGGATGAAACTACTTTGT+CGG + chr1.2:9110311-9110330 None:intergenic 40.0%
CCAAAGAAGTCGAAGAAAAC+AGG + chr1.2:9109613-9109632 None:intergenic 40.0%
CTACAGATTCTTTCTCTGTC+AGG - chr1.2:9110508-9110527 MS.gene33202:intron 40.0%
CTAGCTGTTATTGGACACTA+TGG - chr1.2:9109680-9109699 MS.gene33202:CDS 40.0%
CTCGAAATTCTGAACCTTGA+AGG - chr1.2:9110352-9110371 MS.gene33202:intron 40.0%
GAAAGTGACTGTGAAGAGTT+TGG - chr1.2:9109116-9109135 MS.gene33202:CDS 40.0%
GATCTCTCTCTAGCTGTTAT+TGG - chr1.2:9109671-9109690 MS.gene33202:CDS 40.0%
GCAAATTTCTTCCTGTCTCT+TGG - chr1.2:9108819-9108838 MS.gene33202:CDS 40.0%
GCACAATCGTTTATGTTCTC+AGG - chr1.2:9108753-9108772 MS.gene33202:CDS 40.0%
GCCACAGAATTACCCAATTT+GGG - chr1.2:9109975-9109994 MS.gene33202:CDS 40.0%
GCTGGATATTTAGTCCCAAA+AGG + chr1.2:9110588-9110607 None:intergenic 40.0%
GGATAGTAGACATCAACTTG+AGG + chr1.2:9108846-9108865 None:intergenic 40.0%
TATTTAGTCCCAAAAGGGTG+TGG + chr1.2:9110582-9110601 None:intergenic 40.0%
TGCCACAGAATTACCCAATT+TGG - chr1.2:9109974-9109993 MS.gene33202:CDS 40.0%
TGGGTTATGGGAAATGCTAA+TGG - chr1.2:9109761-9109780 MS.gene33202:CDS 40.0%
TGTGCATTTCTCTCAGACAA+CGG - chr1.2:9109896-9109915 MS.gene33202:CDS 40.0%
TTCAAGTGATATAGCAGCCT+TGG + chr1.2:9109936-9109955 None:intergenic 40.0%
! AAATTGCAGGGCATTTGCAA+AGG + chr1.2:9109415-9109434 None:intergenic 40.0%
! AACTGCGATTAAAGCCTTGT+CGG + chr1.2:9109438-9109457 None:intergenic 40.0%
! ATCTTTAGAAGGTTGCCTGT+TGG - chr1.2:9108975-9108994 MS.gene33202:CDS 40.0%
! CAGGAAGAAATTTGCTTCCT+TGG + chr1.2:9108814-9108833 None:intergenic 40.0%
! CCTGTTTTCTTCGACTTCTT+TGG - chr1.2:9109610-9109629 MS.gene33202:CDS 40.0%
! CGGATTGATTTCATTCACCA+AGG - chr1.2:9109916-9109935 MS.gene33202:CDS 40.0%
! GGAAGAAATTTGCTTCCTTG+GGG + chr1.2:9108812-9108831 None:intergenic 40.0%
! GTGATAACTTTTGCCATGGT+GGG - chr1.2:9108775-9108794 MS.gene33202:CDS 40.0%
! TCAGGTGATAACTTTTGCCA+TGG - chr1.2:9108771-9108790 MS.gene33202:CDS 40.0%
! TGCTGATTGAATTGCAATGC+TGG + chr1.2:9110606-9110625 None:intergenic 40.0%
! TGCTTGTTGAACATCTTTGG+AGG - chr1.2:9110641-9110660 MS.gene33202:intron 40.0%
!! CTTACCCGCTTTGAGAAAAT+TGG - chr1.2:9110555-9110574 MS.gene33202:intron 40.0%
!! GACTTCTTTGGTTCTGACAA+AGG - chr1.2:9109622-9109641 MS.gene33202:CDS 40.0%
!! GCCTCTTTGAGATCTTTAGA+AGG - chr1.2:9108964-9108983 MS.gene33202:CDS 40.0%
!! GTTTCTTCTGTTGGTGATGA+AGG - chr1.2:9109344-9109363 MS.gene33202:CDS 40.0%
AAAGGTGAAGCTTCAAGCAC+TGG - chr1.2:9109640-9109659 MS.gene33202:CDS 45.0%
AACGCTACCCTTTCTGTACT+CGG - chr1.2:9110151-9110170 MS.gene33202:intron 45.0%
AGACCTTCATTGCGAATGCT+AGG + chr1.2:9109506-9109525 None:intergenic 45.0%
AGTGACTGTGAAGAGTTTGG+AGG - chr1.2:9109119-9109138 MS.gene33202:CDS 45.0%
ATCTGTAACTGCCCTGGATT+TGG - chr1.2:9110127-9110146 MS.gene33202:intron 45.0%
ATGTTAGTGAGAGAGGCTTC+TGG - chr1.2:9109741-9109760 MS.gene33202:CDS 45.0%
CCTTCTTTCCTTCCAAGCTT+CGG + chr1.2:9109160-9109179 None:intergenic 45.0%
CGACAAAGTAGTTTCATCCC+TGG - chr1.2:9110309-9110328 MS.gene33202:intron 45.0%
CTCCCAAATGTTAGTGAGAG+AGG - chr1.2:9109734-9109753 MS.gene33202:CDS 45.0%
CTCTCTCACTAACATTTGGG+AGG + chr1.2:9109736-9109755 None:intergenic 45.0%
CTTCTTTCCTTCCAAGCTTC+GGG + chr1.2:9109159-9109178 None:intergenic 45.0%
GACGCCGAAAAACCCAAATT+GGG + chr1.2:9109990-9110009 None:intergenic 45.0%
GATGTTGGGATTGAAGCTGT+TGG - chr1.2:9109833-9109852 MS.gene33202:CDS 45.0%
GGGATTGAAGCTGTTGGAAA+GGG - chr1.2:9109839-9109858 MS.gene33202:CDS 45.0%
GGTTTGTGTATAACCCACCA+TGG + chr1.2:9108791-9108810 None:intergenic 45.0%
TGGGATTGAAGCTGTTGGAA+AGG - chr1.2:9109838-9109857 MS.gene33202:CDS 45.0%
TGGGTTATACACAAACCCCA+AGG - chr1.2:9108794-9108813 MS.gene33202:CDS 45.0%
TGTCCTAGCATTCGCAATGA+AGG - chr1.2:9109500-9109519 MS.gene33202:CDS 45.0%
TGTGAAGAGTTTGGAGGTGA+GGG - chr1.2:9109125-9109144 MS.gene33202:CDS 45.0%
TGTGGCACTCTTCCAATTGT+AGG + chr1.2:9109961-9109980 None:intergenic 45.0%
TGTTAGTGAGAGAGGCTTCT+GGG - chr1.2:9109742-9109761 MS.gene33202:CDS 45.0%
TTAAGGGAGTCACAGATGCT+GGG - chr1.2:9110215-9110234 MS.gene33202:intron 45.0%
TTAGGGAAGCTTTCAATCCG+TGG - chr1.2:9109233-9109252 MS.gene33202:CDS 45.0%
TTATCGATCTGTAACTGCCC+TGG - chr1.2:9110121-9110140 MS.gene33202:intron 45.0%
! AAAATTGGGCCACACCCTTT+TGG - chr1.2:9110570-9110589 MS.gene33202:intron 45.0%
! AAAGCCTTGTCGGAAATTGC+AGG + chr1.2:9109428-9109447 None:intergenic 45.0%
! AAATTGGGCCACACCCTTTT+GGG - chr1.2:9110571-9110590 MS.gene33202:intron 45.0%
! AAGCCTTGTCGGAAATTGCA+GGG + chr1.2:9109427-9109446 None:intergenic 45.0%
! GGTGATAACTTTTGCCATGG+TGG - chr1.2:9108774-9108793 MS.gene33202:CDS 45.0%
! GTGGCCCAATTTTCTCAAAG+CGG + chr1.2:9110563-9110582 None:intergenic 45.0%
! TATCTGTTTTGCGTGTGCCA+GGG + chr1.2:9110485-9110504 None:intergenic 45.0%
! TCTCAAGTCTTCGGCTTTTC+TGG - chr1.2:9108162-9108181 MS.gene33202:CDS 45.0%
! TGGCCCAATTTTCTCAAAGC+GGG + chr1.2:9110562-9110581 None:intergenic 45.0%
! TTATCTGTTTTGCGTGTGCC+AGG + chr1.2:9110486-9110505 None:intergenic 45.0%
!! AGACTTGCTGCTATTGCAGT+TGG - chr1.2:9109191-9109210 MS.gene33202:CDS 45.0%
!! ATCTCAAAGAGGCACTCATC+TGG + chr1.2:9108957-9108976 None:intergenic 45.0%
!! GGCCTTATTGAGATCGCTAA+TGG - chr1.2:9109365-9109384 MS.gene33202:CDS 45.0%
!! TTCGGCGTCCTTTTTAACTG+TGG - chr1.2:9110001-9110020 MS.gene33202:CDS 45.0%
!!! ATTTTTTTATGAATTAATTA+TGG - chr1.2:9108352-9108371 MS.gene33202:CDS 5.0%
!!! TTTTTTTATGAATTAATTAT+GGG - chr1.2:9108353-9108372 MS.gene33202:CDS 5.0%
ACTGCATCACGAGGAGGATT+AGG - chr1.2:9109215-9109234 MS.gene33202:CDS 50.0%
ATGCCCTGCAATTTCCGACA+AGG - chr1.2:9109421-9109440 MS.gene33202:CDS 50.0%
CCAACCATGCAGATTGACCA+GGG + chr1.2:9110329-9110348 None:intergenic 50.0%
CCGAAGCTTGGAAGGAAAGA+AGG - chr1.2:9109157-9109176 MS.gene33202:CDS 50.0%
CTCACAATTGCATCCTGCAG+AGG - chr1.2:9109803-9109822 MS.gene33202:CDS 50.0%
CTGACACGTGATCTCTTCTG+AGG + chr1.2:9108870-9108889 None:intergenic 50.0%
CTGCATCACGAGGAGGATTA+GGG - chr1.2:9109216-9109235 MS.gene33202:CDS 50.0%
CTGCTTGTGTTTCCAAACGC+TGG - chr1.2:9109015-9109034 MS.gene33202:CDS 50.0%
CTGTGAAGAGTTTGGAGGTG+AGG - chr1.2:9109124-9109143 MS.gene33202:CDS 50.0%
CTTAAGGGAGTCACAGATGC+TGG - chr1.2:9110214-9110233 MS.gene33202:intron 50.0%
GAGAGAGGCTTCTGGGTTAT+GGG - chr1.2:9109749-9109768 MS.gene33202:CDS 50.0%
GATTCCTTGATCCCCAACAC+CGG + chr1.2:9109585-9109604 None:intergenic 50.0%
GCACTCCTTTCTTCACCAAC+AGG + chr1.2:9108993-9109012 None:intergenic 50.0%
GCAGAGGAGTAACCGATGTT+GGG - chr1.2:9109819-9109838 MS.gene33202:CDS 50.0%
GGACGCCGAAAAACCCAAAT+TGG + chr1.2:9109991-9110010 None:intergenic 50.0%
GGTCTCAAAGCAGTTGCTTG+TGG - chr1.2:9109287-9109306 MS.gene33202:CDS 50.0%
GTCAGGCTGCACTTTGGTTA+CGG - chr1.2:9110525-9110544 MS.gene33202:intron 50.0%
TACCTTTCCCGAAGCTTGGA+AGG - chr1.2:9109149-9109168 MS.gene33202:CDS 50.0%
TCATCCCTGGTCAATCTGCA+TGG - chr1.2:9110322-9110341 MS.gene33202:intron 50.0%
TCCAACCATGCAGATTGACC+AGG + chr1.2:9110330-9110349 None:intergenic 50.0%
TGAGAGAGGCTTCTGGGTTA+TGG - chr1.2:9109748-9109767 MS.gene33202:CDS 50.0%
TGCAGAGGAGTAACCGATGT+TGG - chr1.2:9109818-9109837 MS.gene33202:CDS 50.0%
TGGGATCAAGGATCTGGACT+TGG - chr1.2:9110060-9110079 MS.gene33202:intron 50.0%
TTCCTTCCAAGCTTCGGGAA+AGG + chr1.2:9109154-9109173 None:intergenic 50.0%
TTCTCTGTCAGGCTGCACTT+TGG - chr1.2:9110519-9110538 MS.gene33202:intron 50.0%
TTGTGTGCGATCTCCGATTC+CGG - chr1.2:9110451-9110470 MS.gene33202:intron 50.0%
! AACATCGGTTACTCCTCTGC+AGG + chr1.2:9109819-9109838 None:intergenic 50.0%
! ACACGTGATCTCTTCTGAGG+TGG + chr1.2:9108867-9108886 None:intergenic 50.0%
! CAGAAGCATAAGCCAGCGTT+TGG + chr1.2:9109030-9109049 None:intergenic 50.0%
! GGTTGCCTGTTGGTGAAGAA+AGG - chr1.2:9108985-9109004 MS.gene33202:CDS 50.0%
!! CTGCTTTGGGATCAAGGATC+TGG - chr1.2:9110054-9110073 MS.gene33202:intron 50.0%
!! GGTAAGCTGCTTTGGGATCA+AGG - chr1.2:9110048-9110067 MS.gene33202:intron 50.0%
AGGGTAGCGTTGCCAAATCC+AGG + chr1.2:9110142-9110161 None:intergenic 55.0%
CAACTTGAGGGCCAAGAGAC+AGG + chr1.2:9108833-9108852 None:intergenic 55.0%
CCCTGGTCAATCTGCATGGT+TGG - chr1.2:9110326-9110345 MS.gene33202:intron 55.0%
CGTGGAAGCAACTCAGAACG+CGG - chr1.2:9109251-9109270 MS.gene33202:CDS 55.0%
GCAGTTGGAACTGCATCACG+AGG - chr1.2:9109206-9109225 MS.gene33202:CDS 55.0%
GCTGCACTTTGGTTACGGAC+AGG - chr1.2:9110530-9110549 MS.gene33202:intron 55.0%
GGAATCGGAGATCGCACACA+AGG + chr1.2:9110452-9110471 None:intergenic 55.0%
GGGATACCTTTCCCGAAGCT+TGG - chr1.2:9109145-9109164 MS.gene33202:CDS 55.0%
GGGTAGCGTTGCCAAATCCA+GGG + chr1.2:9110141-9110160 None:intergenic 55.0%
GTTGGAACTGCATCACGAGG+AGG - chr1.2:9109209-9109228 MS.gene33202:CDS 55.0%
GTTGGGGATCAAGGAATCGC+TGG - chr1.2:9109587-9109606 MS.gene33202:CDS 55.0%
TCCATTGCTTGAGAGCTCCG+AGG - chr1.2:9110243-9110262 MS.gene33202:intron 55.0%
TGTGTGCGATCTCCGATTCC+GGG - chr1.2:9110452-9110471 MS.gene33202:intron 55.0%
! ATCAAGGACTGTGCCGGTGT+TGG - chr1.2:9109569-9109588 MS.gene33202:CDS 55.0%
! CGCGTTCTGAGTTGCTTCCA+CGG + chr1.2:9109253-9109272 None:intergenic 55.0%
! TCAAGGACTGTGCCGGTGTT+GGG - chr1.2:9109570-9109589 MS.gene33202:CDS 55.0%
CAGGGCTGCTATCCCGGAAT+CGG + chr1.2:9110467-9110486 None:intergenic 60.0%
GAACGCGGCGTGACAACTCT+AGG - chr1.2:9109266-9109285 MS.gene33202:CDS 60.0%
GCCTCGGAGCTCTCAAGCAA+TGG + chr1.2:9110247-9110266 None:intergenic 60.0%
TTGCTTGAGAGCTCCGAGGC+TGG - chr1.2:9110247-9110266 MS.gene33202:intron 60.0%
! CAAGGACTGTGCCGGTGTTG+GGG - chr1.2:9109571-9109590 MS.gene33202:CDS 60.0%
! TGTGCCGGTGTTGGGGATCA+AGG - chr1.2:9109578-9109597 MS.gene33202:CDS 60.0%
CGATTCCGGGATAGCAGCCC+TGG - chr1.2:9110465-9110484 MS.gene33202:intron 65.0%
GTGTGCCAGGGCTGCTATCC+CGG + chr1.2:9110473-9110492 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 9108159 9110681 9108159 ID=MS.gene33202
chr1.2 mRNA 9108159 9110681 9108159 ID=MS.gene33202.t1;Parent=MS.gene33202
chr1.2 exon 9110657 9110681 9110657 ID=MS.gene33202.t1.exon1;Parent=MS.gene33202.t1
chr1.2 CDS 9110657 9110681 9110657 ID=cds.MS.gene33202.t1;Parent=MS.gene33202.t1
chr1.2 exon 9108159 9110065 9108159 ID=MS.gene33202.t1.exon2;Parent=MS.gene33202.t1
chr1.2 CDS 9108159 9110065 9108159 ID=cds.MS.gene33202.t1;Parent=MS.gene33202.t1
Gene Sequence

>MS.gene33202

ATGTCTCAAGTCTTCGGCTTTTCTGGTAATTTCAATGATCCTTTACGTTTTTTATTTGATTCATAGATTTTTAGGTTGTGGGTGTGTTTTAAATTTTGCAACCAAAAATAGTAGCATGTGGTTCTATAGAATTGATTAGATGATGATTTTGATGTTCATAGGAAAGTTTATTTTTTGATGATAATTTTGATCTATTTTTTTATGAATTAATTATGGGTTTGATCTTAATTCTCTTAATATGTTATGTGGTATGGATAATCATGTGGTTAAGAGGTGAAATTTCAACTGGGTAATATTATTTGTGTTATAGATCTATGAGATTAGTATATGGGAGGGTTTGTATGACATAGTTTTGATTGATTAGCAGATCTGATTAGAGATATAAAGAGATTATAGTTTTGATGAGATAGTGAGATACTTTGGCATAAATTGTTTTAAGAATTTTACTTTTCTGATATCAAAGGATTCTGCTTTGAGAAGTTTGTTTTGCATAAGTTTGATTTAAGCAGTTTTGTTGAATTTGATGGTTATAACTTATACAATAGACATGATCATTATGTTTATATGCTCAATTTGTTCATTCCTGATGATTTTGCACAATCGTTTATGTTCTCAGGTGATAACTTTTGCCATGGTGGGTTATACACAAACCCCAAGGAAGCAAATTTCTTCCTGTCTCTTGGCCCTCAAGTTGATGTCTACTATCCACCTCAGAAGAGATCACGTGTCAGTGTTCCATTTGTTTTCGATGGAGAATGGTTTGAGCAGAAGCAGAAAACATCTATTGAATCTTTGCCAGATGAGTGCCTCTTTGAGATCTTTAGAAGGTTGCCTGTTGGTGAAGAAAGGAGTGCATCTGCTTGTGTTTCCAAACGCTGGCTTATGCTTCTGAGCAATATTTGCAAGAGTGAAATCTGTAGCAACAAAAGTACCTCCTCAAATGACGAGAATAAAATGGAAAGTGACTGTGAAGAGTTTGGAGGTGAGGGATACCTTTCCCGAAGCTTGGAAGGAAAGAAGGCAACCGATGTTAGACTTGCTGCTATTGCAGTTGGAACTGCATCACGAGGAGGATTAGGGAAGCTTTCAATCCGTGGAAGCAACTCAGAACGCGGCGTGACAACTCTAGGTCTCAAAGCAGTTGCTTGTGGATGTCCTTCTCTAAAGTCTTTCTCTTTATGGAATGTTTCTTCTGTTGGTGATGAAGGCCTTATTGAGATCGCTAATGGATGTCAAAAGCTAGAGAAGCTTGACCTTTGCAAATGCCCTGCAATTTCCGACAAGGCTTTAATCGCAGTTGCAAAGAAGTGTCCGACTCTGACCGAGTTATCATTAGAGTCTTGTCCTAGCATTCGCAATGAAGGTCTACAAGCCATTGGAAAGTTCTGCCCAAATCTTAAGGTTATATCTATCAAGGACTGTGCCGGTGTTGGGGATCAAGGAATCGCTGGCCTGTTTTCTTCGACTTCTTTGGTTCTGACAAAGGTGAAGCTTCAAGCACTGGCCGTTTCTGATCTCTCTCTAGCTGTTATTGGACACTATGGCAAAACAGTTACCGATCTTGTCCTTAATTTCCTCCCAAATGTTAGTGAGAGAGGCTTCTGGGTTATGGGAAATGCTAATGGATTGCATAAACTGAAGTCACTCACAATTGCATCCTGCAGAGGAGTAACCGATGTTGGGATTGAAGCTGTTGGAAAGGGCTGTCCTAATTTGAAAAGCGTACATCTTCATAAGTGTGCATTTCTCTCAGACAACGGATTGATTTCATTCACCAAGGCTGCTATATCACTTGAAAGCCTACAATTGGAAGAGTGCCACAGAATTACCCAATTTGGGTTTTTCGGCGTCCTTTTTAACTGTGGTGCAAAATTGAAGGCTTTTTCTATGGTAAGCTGCTTTGGGATCAAGGATCTGGACTTGGAATTGTCACCTGTATCTCATTGTGAATCACTTCGTTCATTATCGATCTGTAACTGCCCTGGATTTGGCAACGCTACCCTTTCTGTACTCGGAAAGTTGTGCCCTCAGCTTCATCAAGTTGAATTAACTGGACTTAAGGGAGTCACAGATGCTGGGTTGCTTCCATTGCTTGAGAGCTCCGAGGCTGGTCTTGTTAAAGTTAATCTCAGTGGTTGCATCAATCTTACCGACAAAGTAGTTTCATCCCTGGTCAATCTGCATGGTTGGACTCTCGAAATTCTGAACCTTGAAGGTTGTAAAAATATCAGCAATGCTAGCTTGATAGCAATTGCTGAGCATTGCCAATTGCTTTGTGATCTTGATGTTTCCTTGTGTGCGATCTCCGATTCCGGGATAGCAGCCCTGGCACACGCAAAACAGATAAATCTACAGATTCTTTCTCTGTCAGGCTGCACTTTGGTTACGGACAGGAGCTTACCCGCTTTGAGAAAATTGGGCCACACCCTTTTGGGACTAAATATCCAGCATTGCAATTCAATCAGCAGCAGCGCTGTTGAGATGCTTGTTGAACATCTTTGGAGGTGTGACATCCTTTCCTGA
Protein sequence

>MS.gene33202.t1

MSQVFGFSGDNFCHGGLYTNPKEANFFLSLGPQVDVYYPPQKRSRVSVPFVFDGEWFEQKQKTSIESLPDECLFEIFRRLPVGEERSASACVSKRWLMLLSNICKSEICSNKSTSSNDENKMESDCEEFGGEGYLSRSLEGKKATDVRLAAIAVGTASRGGLGKLSIRGSNSERGVTTLGLKAVACGCPSLKSFSLWNVSSVGDEGLIEIANGCQKLEKLDLCKCPAISDKALIAVAKKCPTLTELSLESCPSIRNEGLQAIGKFCPNLKVISIKDCAGVGDQGIAGLFSSTSLVLTKVKLQALAVSDLSLAVIGHYGKTVTDLVLNFLPNVSERGFWVMGNANGLHKLKSLTIASCRGVTDVGIEAVGKGCPNLKSVHLHKCAFLSDNGLISFTKAAISLESLQLEECHRITQFGFFGVLFNCGAKLKAFSMVSCFGIKDLDLELSPVSHCESLRSLSICNCPGFGNATLSVLGKLCPQLHQVELTGLKGVTDAGLLPLLESSEAGLVKVNLSGCINLTDKVVSSLVNLHGWTLEILNLEGCKNISNASLIAIAEHCQLLCDLDVSLCAISDSGIAALAHAKQINLQILSLSGCTLVTDRSLPALRKLGHTLLGLNIQHCNSISSSAVEMLVEHLWRCDILS