AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91547


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91547.t1 MTR_1g082215 95.442 351 16 0 1 351 282 632 0.0 661
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91547.t1 AT5G27330 33.032 221 138 3 135 348 408 625 1.02e-22 99.8
MS.gene91547.t1 AT3G05130 27.632 228 133 5 114 338 423 621 6.21e-13 70.1

Find 56 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAATAAGGTGGAGAATT+TGG 0.209500 1.2:+60641090 MS.gene91547:CDS
TGTCTCAGGTTAATCATTAT+AGG 0.291389 1.2:+60640777 MS.gene91547:CDS
CACTGTTGCACATGTGTCTA+AGG 0.328802 1.2:+60641360 MS.gene91547:CDS
ATATGACGAGGCAACAAATA+AGG 0.371426 1.2:+60641078 MS.gene91547:CDS
AATCAATTCAAAGCAAACTT+TGG 0.409026 1.2:+60641324 MS.gene91547:CDS
AGCTAGAGATGAGGTTGAGA+AGG 0.410360 1.2:+60640634 MS.gene91547:CDS
GGATATGGTTAAGAGAGAAT+TGG 0.418650 1.2:+60640577 None:intergenic
TCTGCGTCTGATGCACATAA+AGG 0.420247 1.2:+60641532 MS.gene91547:CDS
GCAGTCACAAGTTGTGGAAA+TGG 0.421287 1.2:+60640880 MS.gene91547:CDS
TTAAGAGAGAATTGGAGAAT+AGG 0.439435 1.2:+60640585 MS.gene91547:CDS
AAAATTGTAGCTAGAGAGTC+TGG 0.443169 1.2:+60640734 MS.gene91547:CDS
TAAGAATGCTGTTGAGGAAG+TGG 0.466160 1.2:+60640943 MS.gene91547:CDS
GAGGTTGAGAAGGTTAAAGT+TGG 0.473556 1.2:+60640644 MS.gene91547:CDS
AATTTGGAGTTGCAAGTTGT+GGG 0.486287 1.2:+60641106 MS.gene91547:CDS
AAACAATGGATTACTGTCTC+AGG 0.487360 1.2:+60640763 MS.gene91547:CDS
TACTGAATTGAAGCTTAAGC+AGG 0.508643 1.2:+60641039 MS.gene91547:CDS
GAATTTGCAGTCACAAGTTG+TGG 0.509082 1.2:+60640874 MS.gene91547:CDS
AGTAGCAGCAGAGGTTAAAC+CGG 0.511423 1.2:-60641569 None:intergenic
GAATTTGGAGTTGCAAGTTG+TGG 0.512655 1.2:+60641105 MS.gene91547:CDS
CACATAAAGGTAAGAACTTG+TGG 0.519666 1.2:+60641545 MS.gene91547:CDS
TGTACTGAAGGGTGTTGTAG+AGG 0.520552 1.2:+60640706 MS.gene91547:CDS
AGCACAAATAGTAGCAGCAG+AGG 0.529268 1.2:-60641578 None:intergenic
TTGAGGAAGTGGAGCTTGAG+AGG 0.534554 1.2:+60640954 MS.gene91547:CDS
CAAGAAAGCATTCAAAGCTA+AGG 0.537974 1.2:+60641462 MS.gene91547:CDS
AATGAGACAAACAAGGAGCT+AGG 0.538433 1.2:+60641196 MS.gene91547:CDS
AAACAAAGTGGAGGATTTGG+AGG 0.546631 1.2:+60641009 MS.gene91547:CDS
ATTCAAAGCTAAGGATGAGC+TGG 0.556043 1.2:+60641471 MS.gene91547:CDS
TGAAGGGTGTTGTAGAGGAG+TGG 0.561592 1.2:+60640711 MS.gene91547:CDS
TGAGAGGGATAACGTCAGAA+AGG 0.563589 1.2:+60640970 MS.gene91547:CDS
TGAGAATGCTGAAGCTGAGT+TGG 0.565780 1.2:+60641144 MS.gene91547:CDS
TGCAGCTCAAAGAGAGTGCA+AGG 0.569535 1.2:+60641273 MS.gene91547:CDS
ATGTTATAAGAATGCTGTTG+AGG 0.573650 1.2:+60640937 MS.gene91547:CDS
GAGAGGGATAACGTCAGAAA+GGG 0.575443 1.2:+60640971 MS.gene91547:CDS
AAAGGTAAGAACTTGTGGAC+CGG 0.591726 1.2:+60641550 MS.gene91547:CDS
AATGAAGTCGTTAAGTTGCA+AGG 0.591972 1.2:+60640674 MS.gene91547:CDS
GTCACAAGTTGTGGAAATGG+AGG 0.603940 1.2:+60640883 MS.gene91547:CDS
TAGGCAGCGTGAGGTTGATG+AGG 0.607173 1.2:+60640604 MS.gene91547:CDS
CAAAGCTAAGGATGAGCTGG+TGG 0.611150 1.2:+60641474 MS.gene91547:CDS
TGAGGAAGTGGAGCTTGAGA+GGG 0.621802 1.2:+60640955 MS.gene91547:CDS
TTGACTGCATATGTTGCTAA+AGG 0.622390 1.2:+60641607 MS.gene91547:CDS
AGGTGAAATAAGTGTACTGA+AGG 0.625603 1.2:+60640694 MS.gene91547:CDS
GTTGCAAGTTGTGGGACTGA+AGG 0.629045 1.2:+60641114 MS.gene91547:CDS
CAACAGTAAGAAGTTTGATG+AGG 0.631997 1.2:+60640835 MS.gene91547:CDS
TGACGAGGCAACAAATAAGG+TGG 0.639359 1.2:+60641081 MS.gene91547:CDS
ACTCTCTTTGAGCTGCAATG+AGG 0.642172 1.2:-60641267 None:intergenic
TGCTGAAGCTGAGTTGGTCA+AGG 0.646705 1.2:+60641150 MS.gene91547:CDS
GGCGAGAAGAGCTAGAGATG+AGG 0.648893 1.2:+60640625 MS.gene91547:CDS
CGTTAATCAAAACATCTATG+CGG 0.652889 1.2:-60641222 None:intergenic
CATCATGAAAAGATATGACG+AGG 0.665707 1.2:+60641066 MS.gene91547:CDS
ACATGCTTCTTCTCACCGCG+AGG 0.668840 1.2:-60641400 None:intergenic
TCGCGGTGAGAAGAAGCATG+TGG 0.678181 1.2:+60641402 MS.gene91547:CDS
GAAGATGAATGAGACAAACA+AGG 0.682202 1.2:+60641189 MS.gene91547:CDS
ATTGGAGAATAGGCAGCGTG+AGG 0.687102 1.2:+60640595 MS.gene91547:CDS
GGTGAAATAAGTGTACTGAA+GGG 0.705436 1.2:+60640695 MS.gene91547:CDS
ACATGTGTCTAAGGAAAGTG+TGG 0.709564 1.2:+60641369 MS.gene91547:CDS
AGTGTGGAAGTGATTCCTCG+CGG 0.788854 1.2:+60641385 MS.gene91547:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATCAATTCAAAGCAAACTT+TGG + chr1.2:60641324-60641343 MS.gene91547:CDS 25.0%
! TGATGAAAAGAAAAACAAAG+TGG + chr1.2:60640997-60641016 MS.gene91547:CDS 25.0%
!!! AGATGTTTTGATTAACGAAA+AGG + chr1.2:60641228-60641247 MS.gene91547:CDS 25.0%
AACAAATAAGGTGGAGAATT+TGG + chr1.2:60641090-60641109 MS.gene91547:CDS 30.0%
ATGTTATAAGAATGCTGTTG+AGG + chr1.2:60640937-60640956 MS.gene91547:CDS 30.0%
CGTTAATCAAAACATCTATG+CGG - chr1.2:60641225-60641244 None:intergenic 30.0%
TGAAAAGAAAAACAAAGTGG+AGG + chr1.2:60641000-60641019 MS.gene91547:CDS 30.0%
TGTCTCAGGTTAATCATTAT+AGG + chr1.2:60640777-60640796 MS.gene91547:CDS 30.0%
TTAAGAGAGAATTGGAGAAT+AGG + chr1.2:60640585-60640604 MS.gene91547:CDS 30.0%
AAAATTGTAGCTAGAGAGTC+TGG + chr1.2:60640734-60640753 MS.gene91547:CDS 35.0%
AGGTGAAATAAGTGTACTGA+AGG + chr1.2:60640694-60640713 MS.gene91547:CDS 35.0%
ATATGACGAGGCAACAAATA+AGG + chr1.2:60641078-60641097 MS.gene91547:CDS 35.0%
CAACAGTAAGAAGTTTGATG+AGG + chr1.2:60640835-60640854 MS.gene91547:CDS 35.0%
CACATAAAGGTAAGAACTTG+TGG + chr1.2:60641545-60641564 MS.gene91547:CDS 35.0%
CATCATGAAAAGATATGACG+AGG + chr1.2:60641066-60641085 MS.gene91547:CDS 35.0%
GAAAAACAAAGTGGAGGATT+TGG + chr1.2:60641006-60641025 MS.gene91547:CDS 35.0%
GAAGATGAATGAGACAAACA+AGG + chr1.2:60641189-60641208 MS.gene91547:CDS 35.0%
GGTGAAATAAGTGTACTGAA+GGG + chr1.2:60640695-60640714 MS.gene91547:CDS 35.0%
TACTGAATTGAAGCTTAAGC+AGG + chr1.2:60641039-60641058 MS.gene91547:CDS 35.0%
TGATGAGGAGAAAAAGAAAG+TGG + chr1.2:60640850-60640869 MS.gene91547:CDS 35.0%
TTGACTGCATATGTTGCTAA+AGG + chr1.2:60641607-60641626 MS.gene91547:CDS 35.0%
! AATGAAGTCGTTAAGTTGCA+AGG + chr1.2:60640674-60640693 MS.gene91547:CDS 35.0%
! AATTTGGAGTTGCAAGTTGT+GGG + chr1.2:60641106-60641125 MS.gene91547:CDS 35.0%
! CTCGTCATATCTTTTCATGA+TGG - chr1.2:60641068-60641087 None:intergenic 35.0%
!! AAACAATGGATTACTGTCTC+AGG + chr1.2:60640763-60640782 MS.gene91547:CDS 35.0%
!! CAAGAAAGCATTCAAAGCTA+AGG + chr1.2:60641462-60641481 MS.gene91547:CDS 35.0%
AAACAAAGTGGAGGATTTGG+AGG + chr1.2:60641009-60641028 MS.gene91547:CDS 40.0%
AAAGGTAAGAACTTGTGGAC+CGG + chr1.2:60641550-60641569 MS.gene91547:CDS 40.0%
AATGAGACAAACAAGGAGCT+AGG + chr1.2:60641196-60641215 MS.gene91547:CDS 40.0%
ACATGTGTCTAAGGAAAGTG+TGG + chr1.2:60641369-60641388 MS.gene91547:CDS 40.0%
ATTCAAAGCTAAGGATGAGC+TGG + chr1.2:60641471-60641490 MS.gene91547:CDS 40.0%
GAATTTGCAGTCACAAGTTG+TGG + chr1.2:60640874-60640893 MS.gene91547:CDS 40.0%
GAGGTTGAGAAGGTTAAAGT+TGG + chr1.2:60640644-60640663 MS.gene91547:CDS 40.0%
GAGTCTGGAGAGAAAAACAA+TGG + chr1.2:60640749-60640768 MS.gene91547:CDS 40.0%
TAAGAATGCTGTTGAGGAAG+TGG + chr1.2:60640943-60640962 MS.gene91547:CDS 40.0%
! GAATTTGGAGTTGCAAGTTG+TGG + chr1.2:60641105-60641124 MS.gene91547:CDS 40.0%
! GTCTTTTGCTGAAGAACTTG+AGG + chr1.2:60641435-60641454 MS.gene91547:CDS 40.0%
ACTCTCTTTGAGCTGCAATG+AGG - chr1.2:60641270-60641289 None:intergenic 45.0%
AGCACAAATAGTAGCAGCAG+AGG - chr1.2:60641581-60641600 None:intergenic 45.0%
AGCTAGAGATGAGGTTGAGA+AGG + chr1.2:60640634-60640653 MS.gene91547:CDS 45.0%
AGTAGCAGCAGAGGTTAAAC+CGG - chr1.2:60641572-60641591 None:intergenic 45.0%
CACTGTTGCACATGTGTCTA+AGG + chr1.2:60641360-60641379 MS.gene91547:CDS 45.0%
GAGAGGGATAACGTCAGAAA+GGG + chr1.2:60640971-60640990 MS.gene91547:CDS 45.0%
GCAGTCACAAGTTGTGGAAA+TGG + chr1.2:60640880-60640899 MS.gene91547:CDS 45.0%
GTCACAAGTTGTGGAAATGG+AGG + chr1.2:60640883-60640902 MS.gene91547:CDS 45.0%
TCTGCGTCTGATGCACATAA+AGG + chr1.2:60641532-60641551 MS.gene91547:CDS 45.0%
TGACGAGGCAACAAATAAGG+TGG + chr1.2:60641081-60641100 MS.gene91547:CDS 45.0%
TGAGAATGCTGAAGCTGAGT+TGG + chr1.2:60641144-60641163 MS.gene91547:CDS 45.0%
TGAGAGGGATAACGTCAGAA+AGG + chr1.2:60640970-60640989 MS.gene91547:CDS 45.0%
!! TGTACTGAAGGGTGTTGTAG+AGG + chr1.2:60640706-60640725 MS.gene91547:CDS 45.0%
AGTGTGGAAGTGATTCCTCG+CGG + chr1.2:60641385-60641404 MS.gene91547:CDS 50.0%
ATTGGAGAATAGGCAGCGTG+AGG + chr1.2:60640595-60640614 MS.gene91547:CDS 50.0%
CAAAGCTAAGGATGAGCTGG+TGG + chr1.2:60641474-60641493 MS.gene91547:CDS 50.0%
GTTGCAAGTTGTGGGACTGA+AGG + chr1.2:60641114-60641133 MS.gene91547:CDS 50.0%
TGAGGAAGTGGAGCTTGAGA+GGG + chr1.2:60640955-60640974 MS.gene91547:CDS 50.0%
TTGAGGAAGTGGAGCTTGAG+AGG + chr1.2:60640954-60640973 MS.gene91547:CDS 50.0%
! TGCAGCTCAAAGAGAGTGCA+AGG + chr1.2:60641273-60641292 MS.gene91547:CDS 50.0%
!! TGAAGGGTGTTGTAGAGGAG+TGG + chr1.2:60640711-60640730 MS.gene91547:CDS 50.0%
!! TGCTGAAGCTGAGTTGGTCA+AGG + chr1.2:60641150-60641169 MS.gene91547:CDS 50.0%
ACATGCTTCTTCTCACCGCG+AGG - chr1.2:60641403-60641422 None:intergenic 55.0%
GGCGAGAAGAGCTAGAGATG+AGG + chr1.2:60640625-60640644 MS.gene91547:CDS 55.0%
! TCGCGGTGAGAAGAAGCATG+TGG + chr1.2:60641402-60641421 MS.gene91547:CDS 55.0%
!! TAGGCAGCGTGAGGTTGATG+AGG + chr1.2:60640604-60640623 MS.gene91547:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 60640581 60641636 60640581 ID=MS.gene91547
chr1.2 mRNA 60640581 60641636 60640581 ID=MS.gene91547.t1;Parent=MS.gene91547
chr1.2 exon 60640581 60641636 60640581 ID=MS.gene91547.t1.exon1;Parent=MS.gene91547.t1
chr1.2 CDS 60640581 60641636 60640581 ID=cds.MS.gene91547.t1;Parent=MS.gene91547.t1
Gene Sequence

>MS.gene91547

ATGGTTAAGAGAGAATTGGAGAATAGGCAGCGTGAGGTTGATGAGGCGAGAAGAGCTAGAGATGAGGTTGAGAAGGTTAAAGTTGGCTATGAGAATGAAGTCGTTAAGTTGCAAGGTGAAATAAGTGTACTGAAGGGTGTTGTAGAGGAGTGGAAAATTGTAGCTAGAGAGTCTGGAGAGAAAAACAATGGATTACTGTCTCAGGTTAATCATTATAGGAATGTTATTGATGAAGTGAAGTCTATGAATGAGCTCAACAGTAAGAAGTTTGATGAGGAGAAAAAGAAAGTGGAGAATTTGCAGTCACAAGTTGTGGAAATGGAGGAAAAAATTGAGCTATTACTCTCTCAAGTTCAATGTTATAAGAATGCTGTTGAGGAAGTGGAGCTTGAGAGGGATAACGTCAGAAAGGGATATGATGAAAAGAAAAACAAAGTGGAGGATTTGGAGGCACATGTTACTGAATTGAAGCTTAAGCAGGATACCATCATGAAAAGATATGACGAGGCAACAAATAAGGTGGAGAATTTGGAGTTGCAAGTTGTGGGACTGAAGGAAAAAATTGAGAATGCTGAAGCTGAGTTGGTCAAGGTGAGTAGCGAGAAAGAGAAGATGAATGAGACAAACAAGGAGCTAGGAAGCCGCATAGATGTTTTGATTAACGAAAAGGATGCTACACATAGTAGCCTCATTGCAGCTCAAAGAGAGTGCAAGGATTTGAGAGCTAAGTTTGAGTCTTCTAGAATCAATTCAAAGCAAACTTTGGAATTGTTGAAGAGCACTGTTGCACATGTGTCTAAGGAAAGTGTGGAAGTGATTCCTCGCGGTGAGAAGAAGCATGTGGAAGAAATTCAGTCTTTTGCTGAAGAACTTGAGGCAATCAAGAAAGCATTCAAAGCTAAGGATGAGCTGGTGGATGATATGAAGAAACAAGTTGTATCACTGCAGAAATCTGCGTCTGATGCACATAAAGGTAAGAACTTGTGGACCGGTTTAACCTCTGCTGCTACTATTTGTGCTTTTGCGTTGACTGCATATGTTGCTAAAGGACGTTGA

Protein sequence

>MS.gene91547.t1

MVKRELENRQREVDEARRARDEVEKVKVGYENEVVKLQGEISVLKGVVEEWKIVARESGEKNNGLLSQVNHYRNVIDEVKSMNELNSKKFDEEKKKVENLQSQVVEMEEKIELLLSQVQCYKNAVEEVELERDNVRKGYDEKKNKVEDLEAHVTELKLKQDTIMKRYDEATNKVENLELQVVGLKEKIENAEAELVKVSSEKEKMNETNKELGSRIDVLINEKDATHSSLIAAQRECKDLRAKFESSRINSKQTLELLKSTVAHVSKESVEVIPRGEKKHVEEIQSFAEELEAIKKAFKAKDELVDDMKKQVVSLQKSASDAHKGKNLWTGLTSAATICAFALTAYVAKGR