Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33228.t1 | RHN51483.1 | 78.6 | 56 | 12 | 0 | 2 | 57 | 560 | 615 | 6.40E-17 | 95.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33228.t1 | A0A072TKK1 | 78.6 | 56 | 12 | 0 | 2 | 57 | 342 | 397 | 4.6e-17 | 95.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049586 | MS.gene33228 | 0.800583 | 1.35E-48 | -1.69E-46 |
| MS.gene050430 | MS.gene33228 | 0.807253 | 5.53E-50 | -1.69E-46 |
| MS.gene055056 | MS.gene33228 | 0.813048 | 3.12E-51 | -1.69E-46 |
| MS.gene055058 | MS.gene33228 | 0.809491 | 1.84E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene33228 | 0.804528 | 2.07E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene33228 | 0.844953 | 5.49E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33228.t1 | MTR_0057s0200 | 78.571 | 56 | 12 | 0 | 2 | 57 | 1 | 56 | 3.95e-28 | 95.1 |
| MS.gene33228.t1 | MTR_5g077900 | 78.571 | 56 | 12 | 0 | 2 | 57 | 168 | 223 | 1.11e-26 | 96.3 |
| MS.gene33228.t1 | MTR_0002s1270 | 78.571 | 56 | 12 | 0 | 2 | 57 | 342 | 397 | 7.89e-26 | 97.1 |
| MS.gene33228.t1 | MTR_4g066320 | 71.930 | 57 | 15 | 1 | 2 | 57 | 62 | 118 | 1.12e-23 | 85.9 |
| MS.gene33228.t1 | MTR_7g046380 | 82.979 | 47 | 8 | 0 | 2 | 48 | 350 | 396 | 2.01e-22 | 87.8 |
| MS.gene33228.t1 | MTR_2g086200 | 80.851 | 47 | 9 | 0 | 11 | 57 | 185 | 231 | 1.60e-20 | 80.5 |
| MS.gene33228.t1 | MTR_3g028430 | 60.784 | 51 | 11 | 1 | 7 | 57 | 15 | 56 | 8.59e-14 | 58.9 |
| MS.gene33228.t1 | MTR_2g092960 | 46.429 | 56 | 30 | 0 | 2 | 57 | 1161 | 1216 | 1.03e-12 | 60.5 |
| MS.gene33228.t1 | MTR_0462s0020 | 47.273 | 55 | 29 | 0 | 2 | 56 | 237 | 291 | 3.09e-12 | 58.9 |
| MS.gene33228.t1 | MTR_4g066300 | 73.333 | 30 | 8 | 0 | 2 | 31 | 9 | 38 | 1.95e-11 | 53.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 5 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCCACAATCAGTTGTGTT+AGG | 0.390652 | 6.3:-60505685 | None:intergenic |
| CTGCTAATATTACTACCTCT+TGG | 0.458064 | 6.3:+60505740 | MS.gene33228:CDS |
| CAACCTAACACAACTGATTG+TGG | 0.514100 | 6.3:+60505682 | MS.gene33228:CDS |
| TATTACTACCTCTTGGATGA+AGG | 0.535487 | 6.3:+60505747 | MS.gene33228:CDS |
| ATGGTATACCTTCATCCAAG+AGG | 0.683941 | 6.3:-60505755 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAGTAAAATAACAATA+AGG | - | chr6.3:60505826-60505845 | None:intergenic | 10.0% |
| !!! | TCTTAATTTGGAAATATATA+TGG | - | chr6.3:60505790-60505809 | None:intergenic | 15.0% |
| !! | ATATATATGGTTCAAACATA+TGG | - | chr6.3:60505777-60505796 | None:intergenic | 20.0% |
| ! | TAATCTATTGTACAAACTGT+AGG | + | chr6.3:60505846-60505865 | MS.gene33228:intron | 25.0% |
| ! | TATGTCTAGCTATCTTAATT+TGG | - | chr6.3:60505802-60505821 | None:intergenic | 25.0% |
| CTGCTAATATTACTACCTCT+TGG | + | chr6.3:60505740-60505759 | MS.gene33228:CDS | 35.0% | |
| TATCCACAATCAGTTGTGTT+AGG | - | chr6.3:60505688-60505707 | None:intergenic | 35.0% | |
| TATTACTACCTCTTGGATGA+AGG | + | chr6.3:60505747-60505766 | MS.gene33228:CDS | 35.0% | |
| ATGGTATACCTTCATCCAAG+AGG | - | chr6.3:60505758-60505777 | None:intergenic | 40.0% | |
| CAACCTAACACAACTGATTG+TGG | + | chr6.3:60505682-60505701 | MS.gene33228:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 60505679 | 60505951 | 60505679 | ID=MS.gene33228 |
| chr6.3 | mRNA | 60505679 | 60505951 | 60505679 | ID=MS.gene33228.t1;Parent=MS.gene33228 |
| chr6.3 | exon | 60505679 | 60505768 | 60505679 | ID=MS.gene33228.t1.exon1;Parent=MS.gene33228.t1 |
| chr6.3 | CDS | 60505679 | 60505768 | 60505679 | ID=cds.MS.gene33228.t1;Parent=MS.gene33228.t1 |
| chr6.3 | exon | 60505868 | 60505951 | 60505868 | ID=MS.gene33228.t1.exon2;Parent=MS.gene33228.t1 |
| chr6.3 | CDS | 60505868 | 60505951 | 60505868 | ID=cds.MS.gene33228.t1;Parent=MS.gene33228.t1 |
| Gene Sequence |
| Protein sequence |
