AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049586

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049586.t1 MTR_3g062320 50.000 958 332 23 1 858 696 1606 0.0 753
MS.gene049586.t1 MTR_3g435040 69.043 533 126 6 1 504 403 925 0.0 719
MS.gene049586.t1 MTR_3g435040 34.446 929 363 31 112 849 741 1614 5.20e-106 359
MS.gene049586.t1 MTR_3g435040 69.043 533 126 6 1 504 403 925 0.0 717
MS.gene049586.t1 MTR_3g435040 27.048 647 310 26 112 653 741 1330 1.92e-30 130
MS.gene049586.t1 MTR_3g062090 70.713 519 123 6 1 494 703 1217 0.0 717
MS.gene049586.t1 MTR_3g062090 28.120 633 289 25 184 681 1074 1675 1.97e-29 127
MS.gene049586.t1 MTR_3g435040 69.043 533 126 6 1 504 403 925 0.0 717
MS.gene049586.t1 MTR_3g435040 27.205 669 323 25 112 653 741 1372 1.98e-33 139
MS.gene049586.t1 MTR_3g062090 70.713 519 123 6 1 494 703 1217 0.0 714
MS.gene049586.t1 MTR_3g062090 28.326 639 290 26 184 685 1074 1681 2.55e-29 126
MS.gene049586.t1 MTR_3g435040 68.785 535 126 7 1 504 403 927 0.0 714
MS.gene049586.t1 MTR_3g435040 34.412 927 366 30 112 849 743 1616 5.97e-106 359
MS.gene049586.t1 MTR_3g435040 68.785 535 126 7 1 504 618 1142 0.0 712
MS.gene049586.t1 MTR_3g435040 34.446 929 363 31 112 849 958 1831 2.43e-105 358
MS.gene049586.t1 MTR_3g435040 68.785 535 126 7 1 504 618 1142 0.0 712
MS.gene049586.t1 MTR_3g435040 34.301 930 366 30 112 849 958 1834 5.06e-105 357
MS.gene049586.t1 MTR_3g435040 68.785 535 126 7 1 504 403 927 0.0 712
MS.gene049586.t1 MTR_3g435040 34.602 867 384 29 112 849 743 1555 3.67e-101 345
MS.gene049586.t1 MTR_3g062420 71.947 524 116 6 1 500 713 1229 0.0 711
MS.gene049586.t1 MTR_3g062420 32.455 949 376 35 112 856 1052 1939 1.63e-87 306
MS.gene049586.t1 MTR_3g062140 73.819 508 89 3 1 502 693 1162 0.0 710
MS.gene049586.t1 MTR_3g062140 32.207 829 369 27 112 828 999 1746 4.95e-79 280
MS.gene049586.t1 MTR_3g435040 68.785 535 126 7 1 504 403 927 0.0 709
MS.gene049586.t1 MTR_3g435040 25.818 550 289 22 112 579 743 1255 1.49e-20 98.2
MS.gene049586.t1 MTR_3g063010 66.667 528 140 6 1 504 710 1225 0.0 679
MS.gene049586.t1 MTR_3g063010 36.223 842 361 31 112 858 1042 1802 7.67e-102 348
MS.gene049586.t1 MTR_3g062970 69.583 480 134 4 1 472 711 1186 0.0 658
MS.gene049586.t1 MTR_3g467640 72.083 480 117 4 1 473 556 1025 0.0 650
MS.gene049586.t1 MTR_3g466750 63.977 533 162 5 1 504 698 1229 0.0 641
MS.gene049586.t1 MTR_3g466750 37.832 867 377 25 112 858 1044 1868 1.70e-126 419
MS.gene049586.t1 MTR_3g452250 62.738 526 139 7 1 504 714 1204 0.0 625
MS.gene049586.t1 MTR_3g452250 62.300 313 67 7 582 858 1582 1879 3.21e-96 332
MS.gene049586.t1 MTR_3g452250 27.604 576 301 25 112 620 1024 1550 1.77e-23 107
MS.gene049586.t1 MTR_3g451150 62.548 526 140 7 1 504 714 1204 0.0 624
MS.gene049586.t1 MTR_3g451150 62.300 313 67 7 582 858 1582 1879 3.21e-96 332
MS.gene049586.t1 MTR_3g451150 27.604 576 301 25 112 620 1024 1550 1.77e-23 107
MS.gene049586.t1 MTR_3g062340 60.489 491 111 9 1 419 714 1193 5.52e-157 504
MS.gene049586.t1 MTR_3g062340 66.295 359 85 4 535 858 1592 1949 2.56e-127 422
MS.gene049586.t1 MTR_3g062340 27.985 804 354 30 47 702 882 1608 4.33e-47 183
MS.gene049586.t1 MTR_3g062990 68.210 324 93 2 1 318 725 1044 1.41e-134 428
MS.gene049586.t1 MTR_3g464840 50.200 500 184 13 1 487 695 1142 1.20e-125 416
MS.gene049586.t1 MTR_3g464840 38.870 584 250 20 333 845 1168 1715 1.84e-84 296
MS.gene049586.t1 MTR_3g464920 41.906 661 304 17 1 623 703 1321 1.44e-122 407
MS.gene049586.t1 MTR_3g464920 44.893 421 148 13 503 858 1340 1741 8.71e-74 265
MS.gene049586.t1 MTR_3g464950 39.716 705 289 19 1 623 698 1348 9.46e-119 396
MS.gene049586.t1 MTR_3g464950 45.833 432 148 13 503 858 1367 1788 4.90e-78 277
MS.gene049586.t1 MTR_3g062220 85.217 230 33 1 1 229 693 922 2.65e-118 381
MS.gene049586.t1 MTR_3g048690 48.963 482 199 11 1 472 643 1087 1.29e-116 384
MS.gene049586.t1 MTR_3g048690 55.556 180 39 2 584 763 1117 1255 1.96e-43 171
MS.gene049586.t1 MTR_3g464870 45.506 534 199 15 1 512 703 1166 1.88e-113 379
MS.gene049586.t1 MTR_3g464870 24.036 674 343 27 73 618 864 1496 2.16e-11 68.6
MS.gene049586.t1 MTR_3g464870 45.506 534 199 15 1 512 703 1166 4.01e-113 381
MS.gene049586.t1 MTR_3g464870 47.200 375 110 8 562 858 1513 1877 1.09e-75 270
MS.gene049586.t1 MTR_3g464870 24.036 674 343 27 73 618 864 1496 1.98e-11 68.6
MS.gene049586.t1 MTR_3g031750 47.166 441 181 7 90 504 988 1402 6.46e-110 372
MS.gene049586.t1 MTR_3g031750 42.694 438 206 9 51 459 712 1133 1.96e-82 291
MS.gene049586.t1 MTR_3g031750 35.714 196 89 7 496 662 1507 1694 1.28e-12 72.8
MS.gene049586.t1 MTR_3g464810 46.080 523 204 17 1 480 698 1185 1.88e-108 367
MS.gene049586.t1 MTR_3g464810 48.209 363 121 9 523 819 1496 1857 3.88e-74 266
MS.gene049586.t1 MTR_3g464810 25.575 696 363 34 38 654 848 1467 3.96e-16 84.0
MS.gene049586.t1 MTR_3g466710 45.929 479 168 10 1 477 702 1091 8.86e-105 350
MS.gene049586.t1 MTR_3g027360 70.000 250 40 4 574 801 42 278 1.56e-104 324
MS.gene049586.t1 MTR_7g026250 64.808 287 55 3 1 285 685 927 7.06e-103 340
MS.gene049586.t1 MTR_3g062130 45.142 494 158 14 408 858 2 425 2.62e-100 318
MS.gene049586.t1 MTR_4g027125 59.677 310 65 4 1 250 133 442 2.80e-97 311
MS.gene049586.t1 MTR_3g062980 36.533 750 295 26 180 858 59 698 2.83e-95 314
MS.gene049586.t1 MTR_4g027255 59.032 310 67 4 1 250 428 737 3.01e-94 312
MS.gene049586.t1 MTR_0922s0020 63.877 227 53 3 646 858 1 212 4.79e-85 270
MS.gene049586.t1 MTR_3g467340 50.464 323 139 6 1 314 705 1015 1.82e-82 286
MS.gene049586.t1 MTR_0854s0010 47.059 323 149 6 1 313 178 488 2.21e-82 274
MS.gene049586.t1 MTR_3g062010 38.278 546 224 17 335 858 790 1244 6.30e-82 287
MS.gene049586.t1 MTR_3g062010 40.753 292 129 6 1 248 707 998 9.58e-48 184
MS.gene049586.t1 MTR_3g062010 32.902 386 190 9 91 472 698 1018 1.74e-43 171
MS.gene049586.t1 MTR_3g062020 57.812 256 65 1 1 256 559 771 7.46e-82 280
MS.gene049586.t1 MTR_2g049960 58.300 247 77 2 638 858 77 323 2.04e-81 265
MS.gene049586.t1 MTR_3g062180 36.469 606 244 21 323 828 64 628 2.02e-79 270
MS.gene049586.t1 MTR_3g062400 53.791 277 63 4 633 858 30 292 1.76e-78 256
MS.gene049586.t1 MTR_3g048607 48.615 325 142 7 1 313 755 1066 4.79e-75 266
MS.gene049586.t1 MTR_3g466580 52.174 253 116 2 1 251 706 955 8.18e-72 255
MS.gene049586.t1 MTR_3g062230 67.797 177 35 2 349 504 2 177 5.95e-66 234
MS.gene049586.t1 MTR_3g062230 26.978 556 246 24 184 621 20 533 9.75e-25 110
MS.gene049586.t1 MTR_0067s0070 54.852 237 105 1 1 235 707 943 2.58e-65 238
MS.gene049586.t1 MTR_0067s0070 60.390 154 59 2 320 472 941 1093 1.18e-46 181
MS.gene049586.t1 MTR_2g451270 49.798 247 70 3 638 858 33 251 7.61e-60 204
MS.gene049586.t1 MTR_2g023000 35.356 478 175 14 367 808 31 410 3.30e-54 194
MS.gene049586.t1 MTR_1344s0010 38.438 320 119 7 1 313 176 424 2.87e-47 175
MS.gene049586.t1 MTR_3g069220 72.807 114 29 1 670 783 26 137 4.37e-47 165
MS.gene049586.t1 MTR_3g451650 39.319 323 118 7 1 313 709 963 5.25e-47 182
MS.gene049586.t1 MTR_7g026240 66.667 129 36 1 730 858 211 332 4.82e-46 168
MS.gene049586.t1 MTR_3g466520 39.564 321 112 7 1 313 670 916 7.42e-46 178
MS.gene049586.t1 MTR_1147s0010 53.801 171 56 3 676 846 13 160 5.13e-44 156
MS.gene049586.t1 MTR_3g048603 55.147 136 52 3 339 472 1 129 1.04e-38 141
MS.gene049586.t1 MTR_1236s0010 56.115 139 56 3 338 472 1 138 1.42e-38 141
MS.gene049586.t1 MTR_3g466550 71.429 91 26 0 768 858 22 112 1.43e-37 136
MS.gene049586.t1 MTR_3g063320 85.000 80 12 0 779 858 60 139 3.68e-37 136
MS.gene049586.t1 MTR_3g466530 48.691 191 79 6 325 498 1 189 5.83e-37 149
MS.gene049586.t1 MTR_3g062050 53.947 152 43 4 353 496 1 133 1.51e-36 142
MS.gene049586.t1 MTR_2g450490 71.429 91 26 0 768 858 180 270 3.86e-36 138
MS.gene049586.t1 MTR_5g092190 27.747 537 280 21 4 457 729 1240 1.45e-30 130
MS.gene049586.t1 MTR_0836s0010 49.242 132 62 3 345 472 1 131 8.13e-30 116
MS.gene049586.t1 MTR_5g085400 26.000 650 329 21 62 598 1005 1615 1.21e-28 124
MS.gene049586.t1 MTR_5g085400 29.368 269 131 8 2 251 699 927 1.09e-18 92.4
MS.gene049586.t1 MTR_5g085400 30.864 162 81 6 341 472 784 944 3.90e-11 67.8
MS.gene049586.t1 MTR_1g031830 36.979 192 99 3 329 500 976 1165 1.85e-28 124
MS.gene049586.t1 MTR_1g031830 35.102 245 143 4 1 229 764 1008 6.55e-27 119
MS.gene049586.t1 MTR_3g055230 28.994 507 273 18 1 453 726 1199 2.72e-28 123
MS.gene049586.t1 MTR_3g451630 46.212 132 66 3 345 472 1 131 3.31e-28 111
MS.gene049586.t1 MTR_3g055230 28.994 507 273 18 1 453 726 1199 4.46e-28 122
MS.gene049586.t1 MTR_3g055230 28.994 507 273 18 1 453 726 1199 4.68e-28 122
MS.gene049586.t1 MTR_3g053200 27.237 514 276 21 1 453 714 1190 1.06e-27 121
MS.gene049586.t1 MTR_3g055230 33.457 269 151 6 1 251 726 984 1.32e-25 114
MS.gene049586.t1 MTR_3g466620 58.586 99 40 1 1 98 716 814 2.25e-24 110
MS.gene049586.t1 MTR_3g464830 44.366 142 59 5 172 309 16 141 2.98e-24 100
MS.gene049586.t1 MTR_3g055210 35.319 235 139 5 1 227 713 942 9.05e-24 108
MS.gene049586.t1 MTR_3g053180 31.203 266 166 5 1 251 714 977 7.55e-22 102
MS.gene049586.t1 MTR_3g053180 31.203 266 166 5 1 251 714 977 7.57e-22 102
MS.gene049586.t1 MTR_8g464870 25.573 524 298 18 1 472 721 1204 1.76e-17 88.2
MS.gene049586.t1 MTR_3g062060 40.000 130 46 2 511 608 3 132 4.37e-17 84.0
MS.gene049586.t1 MTR_7g068110 26.250 160 116 1 699 856 59 218 2.33e-11 64.7
MS.gene049586.t1 MTR_3g062390 32.161 199 83 7 408 575 2 179 2.91e-11 63.5
MS.gene049586.t1 MTR_2g072150 23.413 504 316 17 28 487 773 1250 3.50e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 150 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTGACCTTTCTTAATTT+TGG 0.060050 3.2:-58492287 None:intergenic
AAGAAGCCATCTTCCATTTC+TGG 0.146104 3.2:+58491061 MS.gene049586:CDS
GCTCGACAGAATGGATAATT+TGG 0.240679 3.2:+58490484 MS.gene049586:CDS
AAATGCATTGCAAAAGATAT+TGG 0.248077 3.2:+58497058 MS.gene049586:CDS
GAGCAATTGGAATACATAAT+TGG 0.256367 3.2:+58492320 MS.gene049586:CDS
GATAAGCTGAAATACATATT+TGG 0.259042 3.2:+58490935 MS.gene049586:CDS
ACGCATCTCTTCAACTTCTT+TGG 0.259257 3.2:-58497099 None:intergenic
ACAACACTGTTGGCAATAAC+TGG 0.259872 3.2:+58492252 MS.gene049586:CDS
TGAAGATTTAAGTTCTTCTT+TGG 0.267166 3.2:+58496713 MS.gene049586:CDS
GAAAGTCTCCGAAGCTGTTT+TGG 0.282147 3.2:+58497172 MS.gene049586:CDS
TAAAACCCCATTGCTTTCTA+AGG 0.291403 3.2:+58491183 MS.gene049586:CDS
GACATTGTACGATTACATTC+TGG 0.306922 3.2:-58491028 None:intergenic
ATTCATATTGCCTTAACATA+TGG 0.309524 3.2:+58492042 MS.gene049586:CDS
CCTACTGTCGTTGGAGAGAT+TGG 0.310186 3.2:+58490691 MS.gene049586:CDS
AATGGAAGATGGCTTCTTAA+TGG 0.310575 3.2:-58491056 None:intergenic
CAAATGTTTACAATACTTGA+TGG 0.325827 3.2:-58490564 None:intergenic
TCTTTAGATTGAATAGGTTT+AGG 0.327430 3.2:-58490607 None:intergenic
AGGCTCAATATTTCAGAAAC+TGG 0.331699 3.2:+58490811 MS.gene049586:CDS
GCAGGAATTGAGTGGGAATA+TGG 0.336326 3.2:+58494199 MS.gene049586:intron
TCATTCATCATTAGGTCAAT+TGG 0.343060 3.2:+58496035 MS.gene049586:CDS
CTAACACATCCAACACACTA+TGG 0.343716 3.2:-58496917 None:intergenic
AAAGTCTCCAACAACATTCT+TGG 0.352015 3.2:-58492173 None:intergenic
GAACTTGTTGAAAATGTTCC+TGG 0.359553 3.2:+58495448 MS.gene049586:CDS
AGTTAAAGTTTGTGAGCAAT+TGG 0.360088 3.2:+58492307 MS.gene049586:CDS
TCAGCACCTCATTCATCATT+AGG 0.360901 3.2:+58496027 MS.gene049586:CDS
CAATATATATTGCATTTGAT+TGG 0.371765 3.2:-58491115 None:intergenic
AAATGTTGTGTGATATTGTT+TGG 0.390087 3.2:-58492447 None:intergenic
TCATCTTCATCTTCCAGTAT+TGG 0.391900 3.2:+58492379 MS.gene049586:CDS
TCAACCGCAGGACAATTTAA+TGG 0.392830 3.2:+58491207 MS.gene049586:CDS
TAGCTCAGCTAGACAGTTGT+AGG 0.394123 3.2:-58496877 None:intergenic
CATTGCTCCAAGAATGTTGT+TGG 0.394132 3.2:+58492166 MS.gene049586:CDS
GGGAAAATGTCGATGAAATT+CGG 0.397380 3.2:-58491007 None:intergenic
ATACCTTCAGAAGGCTTTGA+AGG 0.406731 3.2:-58496597 None:intergenic
CTTCTTTGGTTACACACTGC+TGG 0.407302 3.2:-58497085 None:intergenic
AGAACGAAGGCCTCCAATAC+TGG 0.408085 3.2:-58492392 None:intergenic
TTCATATTGCCTTAACATAT+GGG 0.412525 3.2:+58492043 MS.gene049586:CDS
TTCACCTTCATCATCATTAG+TGG 0.413717 3.2:-58496410 None:intergenic
ACACACTCTTTAGATTGAAT+AGG 0.414018 3.2:-58490613 None:intergenic
ACAAATGATACTTTCTACTT+TGG 0.415861 3.2:-58494264 None:intergenic
ACATAATAGATGAAAGAAAA+GGG 0.418797 3.2:+58490741 MS.gene049586:CDS
TACATAATAGATGAAAGAAA+AGG 0.422807 3.2:+58490740 MS.gene049586:CDS
CTCGGAAGCATCTCCAGAAA+TGG 0.423130 3.2:-58491074 None:intergenic
GGGAACCAAGCTGGACATCT+TGG 0.431133 3.2:-58490958 None:intergenic
TCTTCATCTTCCAGTATTGG+AGG 0.433725 3.2:+58492382 MS.gene049586:CDS
GTTGATCCTTAGAAAGCAAT+GGG 0.439444 3.2:-58491189 None:intergenic
GATGTGGTTTCGATAAAGAT+TGG 0.440119 3.2:+58496985 MS.gene049586:CDS
CATCCAACACACTATGGGTT+TGG 0.445633 3.2:-58496911 None:intergenic
ATTGGTTCTGATTGTTCTTG+TGG 0.449297 3.2:-58491097 None:intergenic
TTCTTTGGTTACACACTGCT+GGG 0.455224 3.2:-58497084 None:intergenic
ACATTGTACGATTACATTCT+GGG 0.456237 3.2:-58491027 None:intergenic
GAGCACGTTCCCATATGTTA+AGG 0.459154 3.2:-58492052 None:intergenic
TGCTACCGTCAAACCTTCTC+AGG 0.465275 3.2:+58495622 MS.gene049586:CDS
CATAGTGTGTTGGATGTGTT+AGG 0.467513 3.2:+58496918 MS.gene049586:CDS
TCTTAGTATTAAGAAGTGTT+CGG 0.468088 3.2:+58490838 MS.gene049586:CDS
ATCTGAGATGCAATTGAAAA+CGG 0.473806 3.2:-58496573 None:intergenic
TTCTGATTGTTCTTGTGGCT+CGG 0.476698 3.2:-58491092 None:intergenic
AATGTTGTGTGATATTGTTT+GGG 0.481715 3.2:-58492446 None:intergenic
ATCATTCATACCTTGATCCA+TGG 0.485606 3.2:-58490354 None:intergenic
GTCAAAAGTCTTCTCCAAGT+TGG 0.489502 3.2:+58490451 MS.gene049586:CDS
TGATCCATGGGAACAATCCC+AGG 0.490492 3.2:-58490341 None:intergenic
AAATGGATCTCAAAGTAAGC+TGG 0.497107 3.2:-58490382 None:intergenic
CAACACTGTTGGCAATAACT+GGG 0.497169 3.2:+58492253 MS.gene049586:CDS
ATCACAACTTTCAACTGTGA+TGG 0.500775 3.2:-58490915 None:intergenic
GAGGCACTGTAATTGTGAAA+TGG 0.502704 3.2:-58490399 None:intergenic
AACTTGAACTCAATAACTGC+AGG 0.503626 3.2:+58492483 MS.gene049586:CDS
TTCTTCTTTGGTTGTCACAA+GGG 0.506425 3.2:+58496725 MS.gene049586:CDS
ATTACATTATATTCCTGAGA+AGG 0.507157 3.2:-58495635 None:intergenic
GAGAGCAAAGGCCACGATAG+TGG 0.507230 3.2:+58495282 MS.gene049586:CDS
GCATCCCTTAAAAGAAATGA+AGG 0.512863 3.2:-58496825 None:intergenic
AGGTGCTGAAGTTTCTGTCT+TGG 0.515228 3.2:-58496013 None:intergenic
GAGACTCTCCAAAACAGCTT+CGG 0.519316 3.2:-58497180 None:intergenic
TAAACTGTTCAGAGAGTCAA+AGG 0.520089 3.2:-58490531 None:intergenic
GGACACATGCTGATTAAACT+TGG 0.520994 3.2:-58492425 None:intergenic
GAGCTTTAGTACAACCAACT+TGG 0.523462 3.2:-58490465 None:intergenic
CTAAAGAGTGTGTCATTGAA+AGG 0.523542 3.2:+58490623 MS.gene049586:CDS
TGTCTTCCCAAAATTAAGAA+AGG 0.524507 3.2:+58492280 MS.gene049586:CDS
TTCGATAAAGATTGGTTGGA+TGG 0.530095 3.2:+58496993 MS.gene049586:CDS
GAAAGTAAGCTCGAATATTG+AGG 0.531172 3.2:+58496548 MS.gene049586:CDS
TGGTTTCGATAAAGATTGGT+TGG 0.531340 3.2:+58496989 MS.gene049586:CDS
TATAATGAGCAACGTCCACT+TGG 0.531904 3.2:+58495590 MS.gene049586:CDS
TGTACTAAAGCTCGACAGAA+TGG 0.532881 3.2:+58490475 MS.gene049586:CDS
AGATATTCTAATAGCCAAGA+GGG 0.536495 3.2:-58497250 None:intergenic
ACTGTGATGGATTCTAGTGC+TGG 0.537025 3.2:-58490902 None:intergenic
CAACAACATTCTTGGAGCAA+TGG 0.537738 3.2:-58492165 None:intergenic
CCAACACACTATGGGTTTGG+AGG 0.537807 3.2:-58496908 None:intergenic
GGGATTGTTCCCATGGATCA+AGG 0.537833 3.2:+58490344 MS.gene049586:CDS
AGATCATGAGCATAGAGAAA+CGG 0.538336 3.2:-58490878 None:intergenic
GCTGCTTTAAGTGCCCCTCT+TGG 0.541688 3.2:+58497236 MS.gene049586:CDS
TTTGGCCAAGATGTCCAGCT+TGG 0.543248 3.2:+58490953 MS.gene049586:CDS
TTACAAGATGTTCGCAGATG+TGG 0.549714 3.2:+58496969 MS.gene049586:CDS
CATTTATTGCAGGAATTGAG+TGG 0.553799 3.2:+58494191 MS.gene049586:intron
GATAATAACAGCACAAGTCA+AGG 0.553831 3.2:+58490791 MS.gene049586:CDS
ATTTATTGCAGGAATTGAGT+GGG 0.554762 3.2:+58494192 MS.gene049586:intron
TCTTGAGTTATCTCTACGCC+AGG 0.556584 3.2:-58495466 None:intergenic
GTTCTTCTTTGGTTGTCACA+AGG 0.556691 3.2:+58496724 MS.gene049586:CDS
TTCAGAGAGTCAAAGGAAAG+AGG 0.557056 3.2:-58490524 None:intergenic
AGTTCATTATCTGTCTCTGA+TGG 0.560308 3.2:-58495430 None:intergenic
TAACACATCCAACACACTAT+GGG 0.560461 3.2:-58496916 None:intergenic
CCAATCTCTCCAACGACAGT+AGG 0.562561 3.2:-58490691 None:intergenic
GAAGCAGAGGTTCTTAGACT+AGG 0.563477 3.2:+58490287 MS.gene049586:CDS
CATACCTGGGATTGTTCCCA+TGG 0.565611 3.2:+58490337 MS.gene049586:CDS
GAAACAGAGATATCAACATC+GGG 0.567068 3.2:-58490649 None:intergenic
AACAATAAAATACCTTCAGA+AGG 0.567414 3.2:-58496606 None:intergenic
ACATTCTTGGAGCAATGGAT+AGG 0.572842 3.2:-58492160 None:intergenic
GGAGATCATGACAACACTGT+TGG 0.573006 3.2:+58492242 MS.gene049586:CDS
GGTTGATCCTTAGAAAGCAA+TGG 0.573909 3.2:-58491190 None:intergenic
GCATCTCCAGAAATGGAAGA+TGG 0.574895 3.2:-58491067 None:intergenic
ATATTCCTGAGAAGGTTTGA+CGG 0.576725 3.2:-58495627 None:intergenic
GTCGAAATAAGTGTTGAAGA+AGG 0.579126 3.2:+58495973 MS.gene049586:CDS
ATTCCTTCAAAGCCTTCTGA+AGG 0.579968 3.2:+58496594 MS.gene049586:CDS
TCATTCATACCTTGATCCAT+GGG 0.579986 3.2:-58490353 None:intergenic
TAAAGATTGGTTGGATGGTG+TGG 0.583005 3.2:+58496998 MS.gene049586:CDS
ATATTGCACAAGATATGTGA+TGG 0.585415 3.2:-58492089 None:intergenic
AAATGCAATATATATTGTTG+TGG 0.589892 3.2:+58491121 MS.gene049586:CDS
TGTCTTGGCATTAGCAGATG+CGG 0.591144 3.2:-58495998 None:intergenic
CATAATAGATGAAAGAAAAG+GGG 0.591738 3.2:+58490742 MS.gene049586:CDS
GCTTTCTAAGGATCAACCGC+AGG 0.600299 3.2:+58491195 MS.gene049586:CDS
TGATGTTAGCCTACTGTCGT+TGG 0.602978 3.2:+58490682 MS.gene049586:CDS
CTTAGACTAGGAAGAATCGA+GGG 0.603883 3.2:+58490299 MS.gene049586:CDS
TTCACTAAGTAATAGATACA+AGG 0.607781 3.2:+58496848 MS.gene049586:CDS
CCTCCAAACCCATAGTGTGT+TGG 0.615763 3.2:+58496908 MS.gene049586:CDS
CACTGTGCTTAGTGTCAATG+AGG 0.615852 3.2:-58490418 None:intergenic
CAGGGACGGTAAAATCATCA+AGG 0.618571 3.2:+58492502 MS.gene049586:CDS
GAAATGAAGGATGCTTAACA+AGG 0.624132 3.2:-58496812 None:intergenic
GTTACCACTAATGATGATGA+AGG 0.626003 3.2:+58496406 MS.gene049586:CDS
AATTTGGAAGAATTGTGCAA+TGG 0.628591 3.2:+58490500 MS.gene049586:CDS
AATAGATGAAAGAAAAGGGG+AGG 0.628847 3.2:+58490745 MS.gene049586:CDS
TGGTATTGAAAATTCTCCAA+AGG 0.633873 3.2:+58495302 MS.gene049586:CDS
ACTTGAACTCAATAACTGCA+GGG 0.634739 3.2:+58492484 MS.gene049586:CDS
GAAGCACATTATAATAGACA+TGG 0.635744 3.2:+58492220 MS.gene049586:CDS
GTATTGTATGCAAGAAGCAG+AGG 0.638565 3.2:+58490274 None:intergenic
AAGATATTCTAATAGCCAAG+AGG 0.641409 3.2:-58497251 None:intergenic
AGAAAAGGGGAGGAATCGAG+AGG 0.641690 3.2:+58490755 MS.gene049586:CDS
GAACTCAATAACTGCAGGGA+CGG 0.644494 3.2:+58492488 MS.gene049586:CDS
TGAAACAGAGATATCAACAT+CGG 0.647089 3.2:-58490650 None:intergenic
AAGCACATTATAATAGACAT+GGG 0.648129 3.2:+58492221 MS.gene049586:CDS
TCTTAGACTAGGAAGAATCG+AGG 0.669449 3.2:+58490298 MS.gene049586:CDS
GTAGCATCAATTTCTCCAAG+TGG 0.669767 3.2:-58495605 None:intergenic
CAATTACATGAAGAGAGCAA+AGG 0.674437 3.2:+58495270 MS.gene049586:CDS
AACACTGTTGGCAATAACTG+GGG 0.676567 3.2:+58492254 MS.gene049586:CDS
AATTGACCTAATGATGAATG+AGG 0.678315 3.2:-58496033 None:intergenic
TGGCAGATTGTGAAGAACGA+AGG 0.678446 3.2:-58492405 None:intergenic
GTTACCATTAAATTGTCCTG+CGG 0.682827 3.2:-58491211 None:intergenic
GATATTCTAATAGCCAAGAG+GGG 0.685829 3.2:-58497249 None:intergenic
AGATTGGAAATAAATGACTG+TGG 0.685860 3.2:+58490707 MS.gene049586:CDS
TTAGACTAGGAAGAATCGAG+GGG 0.696438 3.2:+58490300 MS.gene049586:CDS
ACAGATTGAGGAGTACCACA+AGG 0.739952 3.2:+58491158 MS.gene049586:CDS
TAGACTAGGAAGAATCGAGG+GGG 0.741535 3.2:+58490301 MS.gene049586:CDS
GATGAAGATGAATCTCAGTG+TGG 0.742648 3.2:-58492367 None:intergenic
TTGATCCTTAGAAAGCAATG+GGG 0.744604 3.2:-58491188 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.2 gene 58490281 58497265 58490281 ID=MS.gene049586
chr3.2 mRNA 58490281 58497265 58490281 ID=MS.gene049586.t1;Parent=MS.gene049586
chr3.2 exon 58490281 58491228 58490281 ID=MS.gene049586.t1.exon1;Parent=MS.gene049586.t1
chr3.2 CDS 58490281 58491228 58490281 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58492035 58492523 58492035 ID=MS.gene049586.t1.exon2;Parent=MS.gene049586.t1
chr3.2 CDS 58492035 58492523 58492035 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58494203 58494275 58494203 ID=MS.gene049586.t1.exon3;Parent=MS.gene049586.t1
chr3.2 CDS 58494203 58494275 58494203 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58495232 58495323 58495232 ID=MS.gene049586.t1.exon4;Parent=MS.gene049586.t1
chr3.2 CDS 58495232 58495323 58495232 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58495415 58495489 58495415 ID=MS.gene049586.t1.exon5;Parent=MS.gene049586.t1
chr3.2 CDS 58495415 58495489 58495415 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58495587 58495652 58495587 ID=MS.gene049586.t1.exon6;Parent=MS.gene049586.t1
chr3.2 CDS 58495587 58495652 58495587 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58495970 58496083 58495970 ID=MS.gene049586.t1.exon7;Parent=MS.gene049586.t1
chr3.2 CDS 58495970 58496083 58495970 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58496373 58496427 58496373 ID=MS.gene049586.t1.exon8;Parent=MS.gene049586.t1
chr3.2 CDS 58496373 58496427 58496373 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58496523 58496615 58496523 ID=MS.gene049586.t1.exon9;Parent=MS.gene049586.t1
chr3.2 CDS 58496523 58496615 58496523 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
chr3.2 exon 58496694 58497265 58496694 ID=MS.gene049586.t1.exon10;Parent=MS.gene049586.t1
chr3.2 CDS 58496694 58497265 58496694 ID=cds.MS.gene049586.t1;Parent=MS.gene049586.t1
Gene Sequence

>MS.gene049586

ATGCAAGAAGCAGAGGTTCTTAGACTAGGAAGAATCGAGGGGGAATGGAAAAACATCATACCTGGGATTGTTCCCATGGATCAAGGTATGAATGATCTAGTCCAGCTTACTTTGAGATCCATTTCACAATTACAGTGCCTCATTGACACTAAGCACAGTGAATCTCAAGTGTCAAAAGTCTTCTCCAAGTTGGTTGTACTAAAGCTCGACAGAATGGATAATTTGGAAGAATTGTGCAATGGTCCTCTTTCCTTTGACTCTCTGAACAGTTTAAAGAAGCTGTCCATCAAGTATTGTAAACATTTGAAAAGCTTATTTAAGTGCAACCTAAACCTATTCAATCTAAAGAGTGTGTCATTGAAAGGATGCCCGATGTTGATATCTCTGTTTCAACAGTCAACTGATGTTAGCCTACTGTCGTTGGAGAGATTGGAAATAAATGACTGTGGATGTCTTGAATACATAATAGATGAAAGAAAAGGGGAGGAATCGAGAGGTGAAATAGTTGATGATAATAACAGCACAAGTCAAGGCTCAATATTTCAGAAACTGGAAGTTCTTAGTATTAAGAAGTGTTCGGAACTTGAATTTGTACTTCCGTTTCTCTATGCTCATGATCTTCCAGCACTAGAATCCATCACAGTTGAAAGTTGTGATAAGCTGAAATACATATTTGGCCAAGATGTCCAGCTTGGTTCCCTCAAAAAAATGGAGCTTGGTGGCATACCGAATTTCATCGACATTTTCCCAGAATGTAATCGTACAATGTCTTTGTCCATTAAGAAGCCATCTTCCATTTCTGGAGATGCTTCCGAGCCACAAGAACAATCAGAACCAATCAAATGCAATATATATTGTTGTGGTAAAAAATATGGGCACAGATTGAGGAGTACCACAAGGACTAAAACCCCATTGCTTTCTAAGGATCAACCGCAGGACAATTTAATGGTAACCCTCTTACCTTCTATTTCTTCTGTGATGTTATATTTTTTTATTTTTTTAATACATACCATTAATCTATGAAGCATGGGGGTACATGTACGGTGCTTATGGTACTTCATTTTAGTTCATTATTCTATTGTGAGTGAAAATTGTGACTTTCTCTTTATAAGGGCAACCCGGTGCACTAGAGCTCCCGCATATGCAGGGTACGGGAAGGGGTCCCACCATTTGGTGTATTGTACGCAGTCTTACCCTGTTTTACACAAGAGGCTGTTTCCAGGACTTGAACCCGTGACCTTTCAGTCACATGACAACAACTTTACCATTTGCGCCAAGGTTACCCCTCTAAAAAGGGCAACCCGGTGTACTAGAGCTCCCGCATATGCAGGGTCCGGCAAGGGGTTATTAATAATATAGTATTATACGTACATAGGAGTTTTTTTTGTGGTTGTAAACAACTAATTTTTTTTTACTATTTAAACTTTTATTGTGATGTTTTTATAATAAATTGAAGAATTTAGCCCTCTCATGTTATATTATATAGCAAAACTCATGATTTTATTGACGTAGCCGTTCTTTAGTTTTTTTAAAAAATAGACGTATTCTTGTACCTGTACTCATATCGTACTGGGTACTGCACCCGTACCTATGCAACATAGCCATTGATACAATTTATTATTTGAAAAAAAAATCATTTTATTAGACATCCTATGCTTGAAGATTACTATTAATGTACATTTTTTATTTGGGTAGCTAGAAATAAGAGATTGTTTAACTTCTATTTTTATTTGGATTGGATGACATGATTGCAGAAATCAAATTCATATTGCCTTAACATATGGGAACGTGCTCAATGTCTTTTAAGACCATCACATATCTTGTGCAATATTAAAAAGATTACACTGAAGAATATTTCGAAGATGAAATCAGTATTCATCCTATCCATTGCTCCAAGAATGTTGTTGGAGACTTTGACAATCAGTAAGTGTGATGAATTGAAGCACATTATAATAGACATGGGAGATCATGACAACACTGTTGGCAATAACTGGGGCACTGTCTTCCCAAAATTAAGAAAGGTCAAAGTTAAAGTTTGTGAGCAATTGGAATACATAATTGGACACTTCACTGATGATCATCAAAACCACACTGAGATTCATCTTCATCTTCCAGTATTGGAGGCCTTCGTTCTTCACAATCTGCCAAGTTTAATCAGCATGTGTCCCAAACAATATCACACAACATTTCCACCTTTGAAAAAACTTGAACTCAATAACTGCAGGGACGGTAAAATCATCAAGGTATCCCTATTTTGTATATCTTTGTTATTTGTGAATCTAAACTTGATGATTTTAAGTCATCATATATTTTTAAATGATTTTTTTTATAAAAAGTTCTTCCACAAGTTTCTCTATTTTTGTTACTTTTTATCATGTTAAAAACATTAAAAAAATTAAAATTCAAAAAATGATTAAAAATGTTTGGTTTCATCATAAACTGTTTGTAGGATAATTTTGTAAGAATTGAAAAATGCGGTTTTTTTATGATTGGGACAAAAAACGAAACAATAATTTTATGTAAACTAAAAACATGTTTAATTTTTTAAACAAGTACATTTTTTAGGGTGTCTATTATGAACCTCTTAAATTTTGTTCAAAGGTGGCTTACCATATTTAGTAAACAATGGCATTTAAATTAATAAAATATGAAGGAAATAAAATTTATTATAATTTACTAAAAAAGTAGGGTCCACCTTTGGATGATCCATATTAAATTTTAGAAAAAATAGTGTCTTTGTGAGAACTCTTATTTACAGAGAAGAGAAACTAAGTGATTGCCACTTAAGTGAAAGACGATGATCATGACAATTACCTATTGAGCTATTATCAGTAGATTGTGTGTTGCCAGTCACCACACACTTCACATTTACTTTTCGTTAGCTAGTAGTTTGTCAAATTGAAGTCTTGACATAACATGAGCTTTAATTTTAAATAGCCATGTCCCGCTATTTTGGCTTGTAGGAGGAACTTGTTGTTGCCCATACATACATAATCATGTTTAGTTAGCCTGTCTTTTTTTGTCGATTTGCATTTGTTGTTGAGCCCTTTCACATTTCTCCAAAAAATAAAATACTTTATGTTGACTCATGATCCATTAGGACAAACTTTATTTTCTGCCATTAACACATACTTCTCTTGACAAATTACTTCGTTGAACTTCAATATGACCTAAACAATTTGGACCGAAGATGGTGTGAGGAATAGAGTGAAGTCTTCTATATATTAATGATTTCTAGGAGTGAAAACTAAGTATTATATTTCAGTCCTTCAAAAAAAAAGTATTATATTTCAGGAATTGAATAATATACAACCACTAATGTGTTTATATAGTAAATTTTAAACTTACTATACTCTAATGAAACGACAAGGAAGAAAATAAAAGGTAACAATAATTATCTATAAAAGTTGAGTTCTATGAAATTTTAAGAATTCGAATTTTTCCATAAAATTGACATTAAACTAAATAGAATAGATTATCAATATTTTTTTTGAATATCATCTCTCTTTGAACCAAACCAAATTTTTTGGGGCAGTCAAATAATATATTAATGGACTAGCCACATATAAAACTGTTAATTATTCAAGATATGTTTTACTTTTGAGATAGCCAATGATACCCATTGTTATAAATCCCTTCATCCTTGTTTATTCAATGGAGGAACATATGTAGGAAGAGGATAGGATTTTGGTTTTTTTTTTTTTTTTAACGAAGGGAGTAAAGGTAGCAGATTGAAAAACAAAAGGGTTGGAGTGTTGAAAATTAAATAACAATATTAATTAAATTAAATAATGTAGTACTTCTTTTGGTTACATTCTAATGTAATAATTTAATCCATAGCATTTTATCATCTCTATTGTAGTTATATGACTTTGCTGTTCGAATTTTATGCATGTGTTTATTACCCATTCATTTATTGCAGGAATTGAGTGGGAATATGGATAATTTTCTCGCTTTGAAGAAACTCATAGTAGAAAATAACTCCAAAGTAGAAAGTATCATTTGTCTGAATGAAATAAATGAACAGCAAATGAACTTAGCTTTGGAAGTCATTCATTTAGATGATCTGCCTATGATGACATGCCTTTTTGTGGGTCCCAAAAATTCGTTTTCCCTCCAAAACCTTACAAGAATAAAAATCATGCGATGTGAAAAAATGAAAATTGTTTTCTCCACTTCTATAATAAGGTGTCTACCACAGTTGTTTGATATGAGAATTGAAGAATGCAACGAGTTGAAGCATATTATTGAAGATGATTTGGAGAGTAAAAAACCCTCAAATTTTATGTCTACAACAAAGACATGCTTCCCAAAGCTAAAAAGACTTACTGTATTAAAGTGCAACAAGTTGAAATATGTCTTTCCAATCTCCATATGTAAAGAGCTTCCTGAGCTAAAGTGTCTAGTGATAAGAGAAGCAGATGAGCTGGATGAAATATTTGCAAATGAAAGTGATGATCACAAAGTGGAGATTCCAAATCTACAATTTGTAGTATTTGAAAATCTTCCAAGCCTCTGTCATGCCCAAGGAATTCAATTTCAAGCTGTAAAACATCGTTTCATACAGAATTGTCAAAAACTGTCTCTGGCTTCAGCATCAACAGCAGACTTCAAACGTGATGTTTCTTTTTTCTGTCTTACATTAGGTTATATCGGTACACACTCTATCCAATATTTTTAAAACTATGACATGTTGGCTGTAAATAATTACATATCATGCATTAATATTTACATCAACAAAAACATACCAGTTGGTTAAAGGATATAAATAATTGAATCTTTTGTACCAAAAAAATAAAAAATAATTGAATCTTCTAAAATGATAGGTAAAATGGTAAAACTTTAATTATTAGGTAGATAGATATACAATTGCAAGTGGACGTTATGATTTTATTATTATGCTTGTCAGTTACTATATAATGATGTTAATCTCTATAATATTTTGCTGTAGATCGTGAATTGTTTGACTATTTGATGACTCTGTTTCGACAATTACATGAAGAGAGCAAAGGCCACGATAGTGGTATTGAAAATTCTCCAAAGGTCAGTACCAATCTATTGATCCATGCATAGCACACCTGAATACAATCACCATCATAATGTATTTGAATCAAATGTGCTTTTTATTGTGCAGAAAAAAATGAAACAACCATCAGAGACAGATAATGAACTTGTTGAAAATGTTCCTGGCGTAGAGATAACTCAAGACGTAATTTGCAGGCCATGTCAATAGTATAAGCTTTAGCTAGTAAGTAATGTACTTTATAGATAACTCAAGATCACTAATTAATGTATTCTTATTACAGGAATATAATGAGCAACGTCCACTTGGAGAAATTGATGCTACCGTCAAACCTTCTCAGGAATATAATGTAATATAGTACCACCTCATTGTGCATAAAAAGCTAAAAAAATAAGCAGTAAAAGAAATAATGTCTACTTAAAAATATGTTGCAACTAATTTACATTTTCTTGTTGTGCAATCAGTTGGAGGGTTCAACGTCCAAAACGAATGTCGCATCAACTTTGTTGACCATTTCAGAAACAACAAAGAATGAGCTACCTATACAATTAGCTTCTTCTAAACAAAAGGCATTTCCTCTCATTTTTCTTGTATTCCTTTTAACACTTTTTCAATGTAGGAATTGAATTATACTACAATCTTAAACATCCATTTAATTTTAAGCAGGGTGTCGAAATAAGTGTTGAAGAAGGAACCGCATCTGCTAATGCCAAGACAGAAACTTCAGCACCTCATTCATCATTAGGTCAATTGGCTTCTTCTAAGAATAAAACTTCACAAGTGAGTTTATTTTACTTAACATAGCCTTTGAGTATTGTTGTCGTGGTTTTTATTATTAAATTTCCATGGGATTTCATTTATAAGAGTCATTTTGATATAACGAGTACATAATTGGATTCTACTTTTCACATTTGGATTATTTTGTTTTTTGTTTTTCTATTTTTCTATAATGAAGTAAGCTTATTTCAGGCGAAGATATATATATATATATATATATATATATAGTGAGGCTGATGCTATAAATATTAAGAATTCATGATAATATCATTCTTTTATATGAATTTTGTAGGAAGGTGGTGATGTTACTACTCAATATGTTGATGTTACCACTAATGATGATGAAGGTGAAGACTCTTTAGATAAATTTTATTTTCTGAAGTTATCTCATCGCGCTTTTCATTTTGAAGTTTAATTCTCTTCTCTCACACCTTTTGTTCAGTTTCTCTAAATGATGATGTTGTCATGAAAGTAAGCTCGAATATTGAGGATCCGTTTTCAATTGCATCTCAGATTCCTTCAAAGCCTTCTGAAGGTATTTTATTGTTTCATTTTATTTTTAATTAGTTTCCTTTGCTCAAATATGCATAATTATACTTTCCTATACTTTCAGGCAACCCTTCTAAAATAGATGAAGATTTAAGTTCTTCTTTGGTTGTCACAAGGGAGCTTGAGAATCTAGTCTCCAAAAATCATTTAGCTGTTGATAACTTGTCTTTGTTGACTGATTTCCTTGTTAAGCATCCTTCATTTCTTTTAAGGGATGCTTCACTAAGTAATAGATACAAGGCTTATGCCTACAACTGTCTAGCTGAGCTATTGAAATTCCTCCAAACCCATAGTGTGTTGGATGTGTTAGGTTCAAGCCAATCTGAGTTTTTTGAGTTATTACAAGATGTTCGCAGATGTGGTTTCGATAAAGATTGGTTGGATGGTGTGGAAAAGCGTGCTTTGTTTCCTGATTTACAATTCTCTCAAAATGCATTGCAAAAGATATTGGATTCCCAGCAGTGTGTAACCAAAGAAGTTGAAGAGATGCGTTTAAAGATTAACATTTTAAATCAACATGTGGAAGATCTTAAGCATCAATTGAAAGTCTCCGAAGCTGTTTTGGAGAGTCTCATTAAACAGAAAGCACAAGTTTTAGAAACAAAGGCTGCTTTAAGTGCCCCTCTTGGCTATTAG
Protein sequence

>MS.gene049586.t1

MQEAEVLRLGRIEGEWKNIIPGIVPMDQGMNDLVQLTLRSISQLQCLIDTKHSESQVSKVFSKLVVLKLDRMDNLEELCNGPLSFDSLNSLKKLSIKYCKHLKSLFKCNLNLFNLKSVSLKGCPMLISLFQQSTDVSLLSLERLEINDCGCLEYIIDERKGEESRGEIVDDNNSTSQGSIFQKLEVLSIKKCSELEFVLPFLYAHDLPALESITVESCDKLKYIFGQDVQLGSLKKMELGGIPNFIDIFPECNRTMSLSIKKPSSISGDASEPQEQSEPIKCNIYCCGKKYGHRLRSTTRTKTPLLSKDQPQDNLMKSNSYCLNIWERAQCLLRPSHILCNIKKITLKNISKMKSVFILSIAPRMLLETLTISKCDELKHIIIDMGDHDNTVGNNWGTVFPKLRKVKVKVCEQLEYIIGHFTDDHQNHTEIHLHLPVLEAFVLHNLPSLISMCPKQYHTTFPPLKKLELNNCRDGKIIKELSGNMDNFLALKKLIVENNSKVENRELFDYLMTLFRQLHEESKGHDSGIENSPKKKMKQPSETDNELVENVPGVEITQDEYNEQRPLGEIDATVKPSQEYNGVEISVEEGTASANAKTETSAPHSSLGQLASSKNKTSQEGGDVTTQYVDVTTNDDEVSLNDDVVMKVSSNIEDPFSIASQIPSKPSEGNPSKIDEDLSSSLVVTRELENLVSKNHLAVDNLSLLTDFLVKHPSFLLRDASLSNRYKAYAYNCLAELLKFLQTHSVLDVLGSSQSEFFELLQDVRRCGFDKDWLDGVEKRALFPDLQFSQNALQKILDSQQCVTKEVEEMRLKINILNQHVEDLKHQLKVSEAVLESLIKQKAQVLETKAALSAPLGY