Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34029.t1 | XP_003591924.2 | 98.2 | 436 | 8 | 0 | 1 | 436 | 1 | 436 | 4.20E-241 | 843.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34029.t1 | Q42884 | 78.1 | 438 | 92 | 2 | 1 | 434 | 1 | 438 | 1.8e-192 | 673.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34029.t1 | G7I3B2 | 98.2 | 436 | 8 | 0 | 1 | 436 | 1 | 436 | 3.0e-241 | 843.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene22482 | MS.gene34029 | PPI |
| MS.gene061932 | MS.gene34029 | PPI |
| MS.gene34029 | MS.gene09785 | PPI |
| MS.gene007486 | MS.gene34029 | PPI |
| MS.gene34029 | MS.gene007485 | PPI |
| MS.gene007487 | MS.gene34029 | PPI |
| MS.gene018518 | MS.gene34029 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34029.t1 | MTR_1g095250 | 98.165 | 436 | 8 | 0 | 1 | 436 | 1 | 436 | 0.0 | 887 |
| MS.gene34029.t1 | MTR_0368s0020 | 91.837 | 49 | 4 | 0 | 224 | 272 | 83 | 131 | 1.05e-24 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34029.t1 | AT1G48850 | 79.439 | 428 | 79 | 3 | 2 | 425 | 3 | 425 | 0.0 | 704 |
| MS.gene34029.t1 | AT1G48850 | 78.729 | 362 | 68 | 3 | 2 | 359 | 3 | 359 | 0.0 | 589 |
| MS.gene34029.t1 | AT1G48850 | 78.729 | 362 | 68 | 3 | 2 | 359 | 3 | 359 | 0.0 | 588 |
Find 127 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATGTATCAGTCTCCTTTC+TGG | 0.119746 | 1.4:+74100126 | None:intergenic |
| CACTTCTTCTGTTTCTAATT+TGG | 0.135458 | 1.4:+74092927 | None:intergenic |
| ATCATCTTCTCTGCATATTC+AGG | 0.226636 | 1.4:+74095464 | None:intergenic |
| GCGAGATGTCATTAGCTTAT+AGG | 0.246076 | 1.4:-74099827 | MS.gene34029:CDS |
| GAGGGATCGGAGATTGGAAT+TGG | 0.269061 | 1.4:+74101303 | None:intergenic |
| TACATATGGGAACCACTTTC+GGG | 0.279701 | 1.4:-74100490 | MS.gene34029:CDS |
| CAATTTGGTAGTGGGTTTGC+AGG | 0.291322 | 1.4:-74094544 | MS.gene34029:intron |
| CACTTTCGGGTTACAACATA+TGG | 0.302913 | 1.4:-74100477 | MS.gene34029:CDS |
| CATGTATCAGTCTCCTTTCT+GGG | 0.318592 | 1.4:+74100127 | None:intergenic |
| TTCATTATTTCTCAGGGTCT+TGG | 0.321630 | 1.4:-74094640 | MS.gene34029:intron |
| GTACATATGGGAACCACTTT+CGG | 0.342196 | 1.4:-74100491 | MS.gene34029:CDS |
| TTTCCTCTTTGATCAGTATT+GGG | 0.342486 | 1.4:+74099959 | None:intergenic |
| GCTTCAAGTTTGTCAAATAC+TGG | 0.357310 | 1.4:+74094613 | None:intergenic |
| ATTTCCTCTTTGATCAGTAT+TGG | 0.358775 | 1.4:+74099958 | None:intergenic |
| TATGTCGTTGCCCGCAACTA+AGG | 0.369065 | 1.4:-74094572 | MS.gene34029:CDS |
| CCTTTCTGGGAGTTGTAATT+CGG | 0.369236 | 1.4:+74100140 | None:intergenic |
| CGGGCAGCTGGGAGTACATA+TGG | 0.371354 | 1.4:-74100504 | MS.gene34029:CDS |
| GTGTTCACTCCCAGTCAAAA+AGG | 0.378540 | 1.4:+74094393 | None:intergenic |
| ACCATTGGAAGGGTTGCTTC+TGG | 0.385048 | 1.4:-74099608 | MS.gene34029:CDS |
| GAAATAATTAACATGAGAAT+AGG | 0.385678 | 1.4:-74094174 | MS.gene34029:CDS |
| CATGTTAATTATTTCTCCAT+TGG | 0.386598 | 1.4:+74094182 | None:intergenic |
| AATTGAAAACCCTTAGTTGC+GGG | 0.393669 | 1.4:+74094562 | None:intergenic |
| CTCGTGGTCAATTAAGTCTT+CGG | 0.395962 | 1.4:+74097716 | None:intergenic |
| TTCTCTGCATATTCAGGGTC+TGG | 0.396812 | 1.4:+74095470 | None:intergenic |
| TTTACAGAGATTCGGGCAGC+TGG | 0.397683 | 1.4:-74100516 | MS.gene34029:intron |
| TAAAATACTCTCAGGAGTTT+CGG | 0.400065 | 1.4:-74100104 | MS.gene34029:CDS |
| GGAGGAGAGGGATCGGAGAT+TGG | 0.418731 | 1.4:+74101297 | None:intergenic |
| GGTACTTGTGGACCAATTGA+TGG | 0.418936 | 1.4:-74093010 | MS.gene34029:CDS |
| GGAAGGAGTGAGGAAGGGTT+TGG | 0.419419 | 1.4:+74101357 | None:intergenic |
| AGGGATCGGAGATTGGAATT+GGG | 0.422790 | 1.4:+74101304 | None:intergenic |
| GTATGGTGTCAGATCAGTTC+AGG | 0.431140 | 1.4:-74099772 | MS.gene34029:intron |
| AAACCGCTCTGGTGGGATAC+AGG | 0.446252 | 1.4:-74094323 | MS.gene34029:intron |
| GGTAGAGGCCATGGTAGCTT+TGG | 0.452564 | 1.4:-74093031 | MS.gene34029:CDS |
| TGACAAACTTGAAGCTGAGT+TGG | 0.456051 | 1.4:-74094605 | MS.gene34029:CDS |
| AGGAAGAGAAAGAATCGGTT+CGG | 0.456406 | 1.4:+74101335 | None:intergenic |
| TCGTGGTCAATTAAGTCTTC+GGG | 0.465818 | 1.4:+74097717 | None:intergenic |
| GCAACCTATGACATGAAGTA+TGG | 0.467320 | 1.4:-74099789 | MS.gene34029:CDS |
| GTGCATGTTGTGCCATCAAT+TGG | 0.469901 | 1.4:+74092998 | None:intergenic |
| GTTGTAATTCGGCTTTGACC+TGG | 0.476712 | 1.4:+74100151 | None:intergenic |
| CAGTATTGGGCACAAATACA+TGG | 0.482313 | 1.4:+74099972 | None:intergenic |
| TTGGGCACAAATACATGGAT+TGG | 0.483473 | 1.4:+74099977 | None:intergenic |
| GGTAGACGGAGGAGAGGGAT+CGG | 0.486339 | 1.4:+74101290 | None:intergenic |
| TGTTTCTAATTTGGCTGACA+AGG | 0.491775 | 1.4:+74092936 | None:intergenic |
| GGAAGAGAAAGAATCGGTTC+GGG | 0.494003 | 1.4:+74101336 | None:intergenic |
| GGTGTTGGATGTGTCATTGA+TGG | 0.495772 | 1.4:-74100438 | MS.gene34029:CDS |
| CCGAATTACAACTCCCAGAA+AGG | 0.500551 | 1.4:-74100140 | MS.gene34029:CDS |
| CGAGACACTGACACTTGATC+AGG | 0.501120 | 1.4:-74097697 | MS.gene34029:intron |
| ATTGGAATTGGGAGAAAGGA+AGG | 0.503710 | 1.4:+74101315 | None:intergenic |
| ATGTCGTTGCCCGCAACTAA+GGG | 0.503957 | 1.4:-74094571 | MS.gene34029:CDS |
| AGGAGTGAGGAAGGGTTTGG+TGG | 0.507069 | 1.4:+74101360 | None:intergenic |
| GAATCTCATGGAGGAGGTGT+TGG | 0.513832 | 1.4:-74100453 | MS.gene34029:CDS |
| AACTCCTATATTTAGGGTGG+TGG | 0.515942 | 1.4:-74099650 | MS.gene34029:intron |
| GGAGATTGGAATTGGGAGAA+AGG | 0.519246 | 1.4:+74101311 | None:intergenic |
| GGGCAGCTGGGAGTACATAT+GGG | 0.522096 | 1.4:-74100503 | MS.gene34029:CDS |
| CATGGTAGCTTTGGTACTTG+TGG | 0.522487 | 1.4:-74093022 | MS.gene34029:CDS |
| TCATCTTCTCTGCATATTCA+GGG | 0.523950 | 1.4:+74095465 | None:intergenic |
| GGCTGAGATACCAACCTCTC+GGG | 0.524544 | 1.4:+74093403 | None:intergenic |
| ACGGAGGTAGACGGAGGAGA+GGG | 0.527565 | 1.4:+74101285 | None:intergenic |
| TTCTTGTAAATCAAAGTTCG+CGG | 0.527748 | 1.4:+74092960 | None:intergenic |
| AAGGAAGGAAGAGAAAGAAT+CGG | 0.529630 | 1.4:+74101330 | None:intergenic |
| GACGGAGGTAGACGGAGGAG+AGG | 0.531416 | 1.4:+74101284 | None:intergenic |
| GATACATGTAAAATACTCTC+AGG | 0.533691 | 1.4:-74100112 | MS.gene34029:CDS |
| TGTCATAGGTTGCATCCGCA+TGG | 0.536508 | 1.4:+74099799 | None:intergenic |
| GACACCATACTTCATGTCAT+AGG | 0.537696 | 1.4:+74099785 | None:intergenic |
| TTAATTGCAGGAGTTACTAC+AGG | 0.538145 | 1.4:-74100004 | MS.gene34029:intron |
| TCTTGTAAATCAAAGTTCGC+GGG | 0.540183 | 1.4:+74092961 | None:intergenic |
| TCTTCTGCTAGGGAAACCAT+TGG | 0.551202 | 1.4:-74099623 | MS.gene34029:CDS |
| GGTTCGGGAAGGAGTGAGGA+AGG | 0.551542 | 1.4:+74101351 | None:intergenic |
| AATCAGGGTGGAATTTCCAA+TGG | 0.556969 | 1.4:-74094198 | MS.gene34029:intron |
| GTGGTGGTAGATCTTCTGCT+AGG | 0.562776 | 1.4:-74099634 | MS.gene34029:CDS |
| GAAACGAGGACGGAGGTAGA+CGG | 0.563398 | 1.4:+74101276 | None:intergenic |
| CCTGTGCACTTGAGAGACAT+AGG | 0.564384 | 1.4:+74097749 | None:intergenic |
| TGCAGCTGTACCCATGGTAG+AGG | 0.565756 | 1.4:-74093046 | MS.gene34029:intron |
| CTCTGAAGCTGATATGCAAG+AGG | 0.568295 | 1.4:-74100394 | MS.gene34029:CDS |
| AGCTACCATGGCCTCTACCA+TGG | 0.568585 | 1.4:+74093035 | None:intergenic |
| ACCAGAAGCAACCCTTCCAA+TGG | 0.569596 | 1.4:+74099607 | None:intergenic |
| CCTATGTCTCTCAAGTGCAC+AGG | 0.572163 | 1.4:-74097749 | MS.gene34029:CDS |
| CAACCTCTCGGGACAACACA+GGG | 0.572864 | 1.4:+74093414 | None:intergenic |
| CATATGTTGTAACCCGAAAG+TGG | 0.573615 | 1.4:+74100478 | None:intergenic |
| ACAAGAACAAACCGCTCTGG+TGG | 0.573699 | 1.4:-74094331 | MS.gene34029:CDS |
| ACAGGGATCATGACGACCAC+GGG | 0.574091 | 1.4:+74093431 | None:intergenic |
| TGCTAGGGAAACCATTGGAA+GGG | 0.574957 | 1.4:-74099618 | MS.gene34029:CDS |
| TGTACCCATGGTAGAGGCCA+TGG | 0.577435 | 1.4:-74093040 | MS.gene34029:CDS |
| GTCATAGGTTGCATCCGCAT+GGG | 0.577755 | 1.4:+74099800 | None:intergenic |
| CTGCTAGGGAAACCATTGGA+AGG | 0.577836 | 1.4:-74099619 | MS.gene34029:CDS |
| GTGAAAGGTGATTCTGTTGG+TGG | 0.578147 | 1.4:-74095419 | MS.gene34029:CDS |
| CAACAGAATCACCTTTCACT+CGG | 0.578783 | 1.4:+74095423 | None:intergenic |
| AGGACAAGAACAAACCGCTC+TGG | 0.581332 | 1.4:-74094334 | MS.gene34029:CDS |
| TGGTGGTAGATCTTCTGCTA+GGG | 0.582067 | 1.4:-74099633 | MS.gene34029:CDS |
| ACAACATATGGAGAATCTCA+TGG | 0.584104 | 1.4:-74100465 | MS.gene34029:CDS |
| TTATATAGATCAAAATGGAA+AGG | 0.585581 | 1.4:-74094359 | MS.gene34029:CDS |
| ATTGATGCTGTCCGAGTGAA+AGG | 0.587265 | 1.4:-74095434 | MS.gene34029:CDS |
| GCTACCATGGCCTCTACCAT+GGG | 0.587349 | 1.4:+74093036 | None:intergenic |
| TGCATATCAGCTTCAGAGAG+AGG | 0.589694 | 1.4:+74100399 | None:intergenic |
| TTACAGAGATTCGGGCAGCT+GGG | 0.592616 | 1.4:-74100515 | MS.gene34029:intron |
| GCTTCAGAGAGAGGGAGGCG+AGG | 0.596196 | 1.4:+74100408 | None:intergenic |
| GCATATCAGCTTCAGAGAGA+GGG | 0.596658 | 1.4:+74100400 | None:intergenic |
| GAAGCAATCTACAGTGACTC+GGG | 0.598566 | 1.4:-74093478 | MS.gene34029:CDS |
| GTGCCCAATACTGATCAAAG+AGG | 0.603337 | 1.4:-74099962 | MS.gene34029:CDS |
| CACAAGTACCAAAGCTACCA+TGG | 0.605561 | 1.4:+74093023 | None:intergenic |
| CACAGGGATCATGACGACCA+CGG | 0.605920 | 1.4:+74093430 | None:intergenic |
| TGAACTTGCAGCTGTACCCA+TGG | 0.607274 | 1.4:-74093052 | MS.gene34029:intron |
| TATGGAGAATCTCATGGAGG+AGG | 0.607485 | 1.4:-74100459 | MS.gene34029:CDS |
| TAGGTTGCATCCGCATGGGA+AGG | 0.607769 | 1.4:+74099804 | None:intergenic |
| ATACTCTCAGGAGTTTCGGA+AGG | 0.610665 | 1.4:-74100100 | MS.gene34029:intron |
| AGAAGCAATCTACAGTGACT+CGG | 0.610730 | 1.4:-74093479 | MS.gene34029:CDS |
| CCAACCTCTCGGGACAACAC+AGG | 0.611393 | 1.4:+74093413 | None:intergenic |
| AGCTTATAGGCCTTCCCATG+CGG | 0.616697 | 1.4:-74099814 | MS.gene34029:CDS |
| AATCGGTTCGGGAAGGAGTG+AGG | 0.619223 | 1.4:+74101347 | None:intergenic |
| GGTGGAGATCGAAGAAGCCA+TGG | 0.622096 | 1.4:+74101378 | None:intergenic |
| GTTCGGGAAGGAGTGAGGAA+GGG | 0.624145 | 1.4:+74101352 | None:intergenic |
| AAATTGAAAACCCTTAGTTG+CGG | 0.624146 | 1.4:+74094561 | None:intergenic |
| TCAGAGAGAGGGAGGCGAGG+AGG | 0.627324 | 1.4:+74100411 | None:intergenic |
| GAGAAAGAATCGGTTCGGGA+AGG | 0.628737 | 1.4:+74101340 | None:intergenic |
| TGCAAGAGGATCTTGACAGA+AGG | 0.632234 | 1.4:-74100380 | MS.gene34029:intron |
| CAAGAACAAACCGCTCTGGT+GGG | 0.632701 | 1.4:-74094330 | MS.gene34029:CDS |
| CTTACTGTAATAGTCGATGT+CGG | 0.643905 | 1.4:+74094144 | None:intergenic |
| TATCAGCTTCAGAGAGAGGG+AGG | 0.644707 | 1.4:+74100403 | None:intergenic |
| TAGATCAAAATGGAAAGGTG+AGG | 0.648351 | 1.4:-74094354 | MS.gene34029:CDS |
| GATCAAGTGTCAGTGTCTCG+TGG | 0.657610 | 1.4:+74097700 | None:intergenic |
| CGAGTGAAAGGTGATTCTGT+TGG | 0.682023 | 1.4:-74095422 | MS.gene34029:CDS |
| GAAACAGAGCTCATAGCCCG+TGG | 0.694287 | 1.4:-74093447 | MS.gene34029:CDS |
| ACGAGGACGGAGGTAGACGG+AGG | 0.699102 | 1.4:+74101279 | None:intergenic |
| GTGTTGTAACATGCATTGTG+AGG | 0.723456 | 1.4:-74095397 | MS.gene34029:CDS |
| GATCCCTGTGTTGTCCCGAG+AGG | 0.731256 | 1.4:-74093417 | MS.gene34029:intron |
| ACATATGGAGAATCTCATGG+AGG | 0.763629 | 1.4:-74100462 | MS.gene34029:CDS |
| ATACCTGTATCCCACCAGAG+CGG | 0.816288 | 1.4:+74094320 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 74092918 | 74101398 | 74092918 | ID=MS.gene34029 |
| chr1.4 | mRNA | 74092918 | 74101398 | 74092918 | ID=MS.gene34029.t1;Parent=MS.gene34029 |
| chr1.4 | exon | 74101260 | 74101398 | 74101260 | ID=MS.gene34029.t1.exon1;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74101260 | 74101398 | 74101260 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74100381 | 74100531 | 74100381 | ID=MS.gene34029.t1.exon2;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74100381 | 74100531 | 74100381 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74100101 | 74100177 | 74100101 | ID=MS.gene34029.t1.exon3;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74100101 | 74100177 | 74100101 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74099958 | 74100016 | 74099958 | ID=MS.gene34029.t1.exon4;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74099958 | 74100016 | 74099958 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74099773 | 74099856 | 74099773 | ID=MS.gene34029.t1.exon5;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74099773 | 74099856 | 74099773 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74099565 | 74099657 | 74099565 | ID=MS.gene34029.t1.exon6;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74099565 | 74099657 | 74099565 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74097698 | 74097778 | 74097698 | ID=MS.gene34029.t1.exon7;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74097698 | 74097778 | 74097698 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74095385 | 74095516 | 74095385 | ID=MS.gene34029.t1.exon8;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74095385 | 74095516 | 74095385 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74094545 | 74094647 | 74094545 | ID=MS.gene34029.t1.exon9;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74094545 | 74094647 | 74094545 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74094324 | 74094418 | 74094324 | ID=MS.gene34029.t1.exon10;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74094324 | 74094418 | 74094324 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74094149 | 74094214 | 74094149 | ID=MS.gene34029.t1.exon11;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74094149 | 74094214 | 74094149 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74093418 | 74093502 | 74093418 | ID=MS.gene34029.t1.exon12;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74093418 | 74093502 | 74093418 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| chr1.4 | exon | 74092918 | 74093063 | 74092918 | ID=MS.gene34029.t1.exon13;Parent=MS.gene34029.t1 |
| chr1.4 | CDS | 74092918 | 74093063 | 74092918 | ID=cds.MS.gene34029.t1;Parent=MS.gene34029.t1 |
| Gene Sequence |
| Protein sequence |
