Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34391.t1 | XP_013469751.1 | 96.8 | 186 | 5 | 1 | 1 | 185 | 1 | 186 | 2.70E-88 | 334.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34391.t1 | P11432 | 72.6 | 197 | 41 | 2 | 1 | 185 | 1 | 196 | 1.7e-67 | 256.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34391.t1 | A0A072VP35 | 96.8 | 186 | 5 | 1 | 1 | 185 | 1 | 186 | 1.9e-88 | 334.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051734 | MS.gene34391 | 0.800296 | 1.54E-48 | -1.69E-46 |
| MS.gene052469 | MS.gene34391 | 0.837608 | 4.70E-57 | -1.69E-46 |
| MS.gene055539 | MS.gene34391 | 0.844891 | 5.71E-59 | -1.69E-46 |
| MS.gene055741 | MS.gene34391 | 0.802321 | 5.93E-49 | -1.69E-46 |
| MS.gene055744 | MS.gene34391 | 0.805992 | 1.02E-49 | -1.69E-46 |
| MS.gene059717 | MS.gene34391 | 0.821248 | 4.47E-53 | -1.69E-46 |
| MS.gene059788 | MS.gene34391 | 0.81995 | 8.87E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene34391 | 0.818868 | 1.57E-52 | -1.69E-46 |
| MS.gene061231 | MS.gene34391 | 0.812771 | 3.59E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34391.t1 | MTR_1g102830 | 96.774 | 186 | 5 | 1 | 1 | 185 | 1 | 186 | 7.85e-129 | 359 |
| MS.gene34391.t1 | MTR_1g102810 | 93.491 | 169 | 11 | 0 | 17 | 185 | 14 | 182 | 1.34e-114 | 323 |
| MS.gene34391.t1 | MTR_1g102780 | 80.447 | 179 | 21 | 1 | 21 | 185 | 22 | 200 | 1.04e-98 | 284 |
| MS.gene34391.t1 | MTR_1g102570 | 79.661 | 177 | 24 | 1 | 21 | 185 | 22 | 198 | 4.82e-97 | 280 |
| MS.gene34391.t1 | MTR_1g102800 | 79.545 | 176 | 24 | 1 | 22 | 185 | 22 | 197 | 1.18e-96 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34391.t1 | AT3G22840 | 50.500 | 200 | 79 | 4 | 1 | 185 | 1 | 195 | 1.57e-58 | 182 |
| MS.gene34391.t1 | AT4G14690 | 51.323 | 189 | 78 | 3 | 11 | 185 | 5 | 193 | 1.63e-54 | 171 |
Find 46 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTCCATTGATCCTTTC+AGG | 0.186558 | 1.2:-76506098 | None:intergenic |
| CAGAAGGGATGTTGTTAAAT+TGG | 0.214729 | 1.2:-76505523 | None:intergenic |
| ATTGTTGACAGTTGCTTCTT+TGG | 0.275064 | 1.2:+76506229 | MS.gene34391:CDS |
| ATTCCAAAGCTCTGCATTTG+AGG | 0.355412 | 1.2:-76506303 | None:intergenic |
| GGTTTGTTCGATCAAATATC+TGG | 0.369233 | 1.2:+76506173 | MS.gene34391:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1.2:+76506337 | MS.gene34391:CDS |
| GGCTGTTACAGAATATGTTA+AGG | 0.394991 | 1.2:+76506358 | MS.gene34391:CDS |
| AATGGAAGGTTGGCCATGAT+TGG | 0.396347 | 1.2:+76506110 | MS.gene34391:CDS |
| GCTGTTACAGAATATGTTAA+GGG | 0.415066 | 1.2:+76506359 | MS.gene34391:CDS |
| TCTTTGGTTCCGTTATTTGA+AGG | 0.415473 | 1.2:+76506245 | MS.gene34391:CDS |
| TAGCTTGAAAGTTCGATCTA+TGG | 0.429335 | 1.2:+76505574 | MS.gene34391:CDS |
| GCAGGCTGTGGTGTCACCTT+TGG | 0.429431 | 1.2:-76505862 | None:intergenic |
| TGTATATGCCTAGCTTTAGA+AGG | 0.430319 | 1.2:+76505546 | MS.gene34391:CDS |
| TCTAGGCATGCTTGAAATGC+TGG | 0.450968 | 1.2:-76505495 | None:intergenic |
| TTGTTAAATTGGTTAACTCT+AGG | 0.465217 | 1.2:-76505512 | None:intergenic |
| TGTCCTCAAATGCAGAGCTT+TGG | 0.467503 | 1.2:+76506300 | MS.gene34391:CDS |
| GAATGGAAGACTTGCTATGT+TGG | 0.490221 | 1.2:+76506322 | MS.gene34391:CDS |
| CTATAAAACTCTATTAAACA+AGG | 0.499792 | 1.2:-76506388 | None:intergenic |
| AATGGAAGACTTGCTATGTT+GGG | 0.502682 | 1.2:+76506323 | MS.gene34391:CDS |
| TGCAGATACAAAACCAATCA+TGG | 0.506046 | 1.2:-76506123 | None:intergenic |
| TTCGATCAAATATCTGGTGG+TGG | 0.522060 | 1.2:+76506179 | MS.gene34391:CDS |
| AGTGTGGAGTCTAAGTCAAA+AGG | 0.525000 | 1.2:+76506272 | MS.gene34391:CDS |
| GCAGCACCTGAAAGGATCAA+TGG | 0.528131 | 1.2:+76506092 | MS.gene34391:CDS |
| ACACTAACACCTTCAAATAA+CGG | 0.528873 | 1.2:-76506254 | None:intergenic |
| CTAGCATTCCTTCTAAAGCT+AGG | 0.532093 | 1.2:-76505554 | None:intergenic |
| TGAAAGGATCAATGGAAGGT+TGG | 0.548007 | 1.2:+76506100 | MS.gene34391:CDS |
| CACTTGTATACAAGAACCAT+GGG | 0.556587 | 1.2:-76506205 | None:intergenic |
| GTTATTTGAAGGTGTTAGTG+TGG | 0.558210 | 1.2:+76506256 | MS.gene34391:CDS |
| CTCTACTACTCGTTCACCAA+AGG | 0.565802 | 1.2:+76505885 | MS.gene34391:CDS |
| GGTGAACGAGTAGTAGAGGC+AGG | 0.568710 | 1.2:-76505880 | None:intergenic |
| TTGGTGGGGCAGCACCTGAA+AGG | 0.571883 | 1.2:+76506084 | MS.gene34391:CDS |
| CGAGTAGTAGAGGCAGGCTG+TGG | 0.578503 | 1.2:-76505874 | None:intergenic |
| TCAAATGCAGAGCTTTGGAA+TGG | 0.580798 | 1.2:+76506305 | MS.gene34391:CDS |
| AAGCTAGGCATATACACAGA+AGG | 0.581115 | 1.2:-76505539 | None:intergenic |
| CATGCTTGAAATGCTGGTCA+TGG | 0.581519 | 1.2:-76505489 | None:intergenic |
| GGTGGAATTAGCAAAAGGGC+AGG | 0.590541 | 1.2:+76506151 | MS.gene34391:CDS |
| TTGTTCGATCAAATATCTGG+TGG | 0.594040 | 1.2:+76506176 | MS.gene34391:CDS |
| AGCTAGGCATATACACAGAA+GGG | 0.602384 | 1.2:-76505538 | None:intergenic |
| GTGGAATTAGCAAAAGGGCA+GGG | 0.608172 | 1.2:+76506152 | MS.gene34391:CDS |
| GAAAGTTCGATCTATGGCTG+AGG | 0.614830 | 1.2:+76505580 | MS.gene34391:CDS |
| ACACTTGTATACAAGAACCA+TGG | 0.618963 | 1.2:-76506206 | None:intergenic |
| CTTTGGTGAACGAGTAGTAG+AGG | 0.621298 | 1.2:-76505884 | None:intergenic |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.2:+76505846 | MS.gene34391:CDS |
| CACCTGAAAGGATCAATGGA+AGG | 0.632072 | 1.2:+76506096 | MS.gene34391:CDS |
| TATAAAACTCTATTAAACAA+GGG | 0.645194 | 1.2:-76506387 | None:intergenic |
| TATCTGGTGGTGGAATCCCA+TGG | 0.688884 | 1.2:+76506189 | MS.gene34391:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATCAAACAAATATTA+AGG | + | chr1.2:76505614-76505633 | MS.gene34391:intron | 10.0% |
| !! | ATCATGATATCATTATATAA+TGG | + | chr1.2:76506027-76506046 | MS.gene34391:intron | 15.0% |
| !!! | GCATATTTAATTTCTATACA+TGG | + | chr1.2:76505660-76505679 | MS.gene34391:intron | 20.0% |
| ! | AATCATAAACGTGTTTGATA+TGG | - | chr1.2:76505694-76505713 | None:intergenic | 25.0% |
| ! | GTCATGTAAAGAATTCATTT+CGG | + | chr1.2:76505970-76505989 | MS.gene34391:intron | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | - | chr1.2:76505515-76505534 | None:intergenic | 25.0% |
| ACACTAACACCTTCAAATAA+CGG | - | chr1.2:76506257-76506276 | None:intergenic | 30.0% | |
| AGTGTCTCTTTACATTATTC+AGG | + | chr1.2:76505822-76505841 | MS.gene34391:intron | 30.0% | |
| CTCTTTACATTATTCAGGAA+GGG | + | chr1.2:76505827-76505846 | MS.gene34391:intron | 30.0% | |
| GCTGTTACAGAATATGTTAA+GGG | + | chr1.2:76506359-76506378 | MS.gene34391:CDS | 30.0% | |
| TCTCTTTACATTATTCAGGA+AGG | + | chr1.2:76505826-76505845 | MS.gene34391:intron | 30.0% | |
| TTACATGACTATACTCTTCT+TGG | - | chr1.2:76505959-76505978 | None:intergenic | 30.0% | |
| TTTATTACTATGCTCACCTT+TGG | - | chr1.2:76505904-76505923 | None:intergenic | 30.0% | |
| !!! | ATTGGTTTTGTATCTGCAAT+GGG | + | chr1.2:76506128-76506147 | MS.gene34391:CDS | 30.0% |
| !!! | GTCGTTGATTTTGTAAATTC+CGG | + | chr1.2:76505741-76505760 | MS.gene34391:intron | 30.0% |
| ACACTTGTATACAAGAACCA+TGG | - | chr1.2:76506209-76506228 | None:intergenic | 35.0% | |
| ATCTATGGCTGAGGTAATTA+AGG | + | chr1.2:76505589-76505608 | MS.gene34391:intron | 35.0% | |
| ATTGTTGACAGTTGCTTCTT+TGG | + | chr1.2:76506229-76506248 | MS.gene34391:CDS | 35.0% | |
| CACTTGTATACAAGAACCAT+GGG | - | chr1.2:76506208-76506227 | None:intergenic | 35.0% | |
| CAGAAGGGATGTTGTTAAAT+TGG | - | chr1.2:76505526-76505545 | None:intergenic | 35.0% | |
| CATAAACGTGTTTGATATGG+TGG | - | chr1.2:76505691-76505710 | None:intergenic | 35.0% | |
| GGCTGTTACAGAATATGTTA+AGG | + | chr1.2:76506358-76506377 | MS.gene34391:CDS | 35.0% | |
| TAGCTTGAAAGTTCGATCTA+TGG | + | chr1.2:76505574-76505593 | MS.gene34391:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.2:76506337-76506356 | MS.gene34391:CDS | 35.0% | |
| TGCAGATACAAAACCAATCA+TGG | - | chr1.2:76506126-76506145 | None:intergenic | 35.0% | |
| TTGTTCGATCAAATATCTGG+TGG | + | chr1.2:76506176-76506195 | MS.gene34391:CDS | 35.0% | |
| ! | CACAAAGTTTTCGGATTTGA+TGG | + | chr1.2:76506058-76506077 | MS.gene34391:CDS | 35.0% |
| ! | GCAGATAAGCACAAAGTTTT+CGG | + | chr1.2:76506049-76506068 | MS.gene34391:intron | 35.0% |
| ! | GGTTTGTTCGATCAAATATC+TGG | + | chr1.2:76506173-76506192 | MS.gene34391:CDS | 35.0% |
| ! | TCTTTGGTTCCGTTATTTGA+AGG | + | chr1.2:76506245-76506264 | MS.gene34391:CDS | 35.0% |
| !! | AATGGAAGACTTGCTATGTT+GGG | + | chr1.2:76506323-76506342 | MS.gene34391:CDS | 35.0% |
| !! | GTTATTTGAAGGTGTTAGTG+TGG | + | chr1.2:76506256-76506275 | MS.gene34391:CDS | 35.0% |
| !! | TGTATATGCCTAGCTTTAGA+AGG | + | chr1.2:76505546-76505565 | MS.gene34391:CDS | 35.0% |
| !! | TTTTCGGATTTGATGGCTTT+TGG | + | chr1.2:76506065-76506084 | MS.gene34391:CDS | 35.0% |
| !!! | GATTGGTTTTGTATCTGCAA+TGG | + | chr1.2:76506127-76506146 | MS.gene34391:CDS | 35.0% |
| !!! | TTGGTTTTGTATCTGCAATG+GGG | + | chr1.2:76506129-76506148 | MS.gene34391:CDS | 35.0% |
| AACCTTCCATTGATCCTTTC+AGG | - | chr1.2:76506101-76506120 | None:intergenic | 40.0% | |
| AAGCTAGGCATATACACAGA+AGG | - | chr1.2:76505542-76505561 | None:intergenic | 40.0% | |
| AGCTAGGCATATACACAGAA+GGG | - | chr1.2:76505541-76505560 | None:intergenic | 40.0% | |
| AGTGTGGAGTCTAAGTCAAA+AGG | + | chr1.2:76506272-76506291 | MS.gene34391:CDS | 40.0% | |
| ATTCCAAAGCTCTGCATTTG+AGG | - | chr1.2:76506306-76506325 | None:intergenic | 40.0% | |
| TGAAAGGATCAATGGAAGGT+TGG | + | chr1.2:76506100-76506119 | MS.gene34391:CDS | 40.0% | |
| TTCGATCAAATATCTGGTGG+TGG | + | chr1.2:76506179-76506198 | MS.gene34391:CDS | 40.0% | |
| !! | CTAGCATTCCTTCTAAAGCT+AGG | - | chr1.2:76505557-76505576 | None:intergenic | 40.0% |
| !! | GAATGGAAGACTTGCTATGT+TGG | + | chr1.2:76506322-76506341 | MS.gene34391:CDS | 40.0% |
| !! | TCAAATGCAGAGCTTTGGAA+TGG | + | chr1.2:76506305-76506324 | MS.gene34391:CDS | 40.0% |
| !!! | TGGTTTTGTATCTGCAATGG+GGG | + | chr1.2:76506130-76506149 | MS.gene34391:CDS | 40.0% |
| ATGGGGGTGGAATTAGCAAA+AGG | + | chr1.2:76506146-76506165 | MS.gene34391:CDS | 45.0% | |
| CACCTGAAAGGATCAATGGA+AGG | + | chr1.2:76506096-76506115 | MS.gene34391:CDS | 45.0% | |
| CATGCTTGAAATGCTGGTCA+TGG | - | chr1.2:76505492-76505511 | None:intergenic | 45.0% | |
| CTCTACTACTCGTTCACCAA+AGG | + | chr1.2:76505885-76505904 | MS.gene34391:CDS | 45.0% | |
| CTTTGGTGAACGAGTAGTAG+AGG | - | chr1.2:76505887-76505906 | None:intergenic | 45.0% | |
| GAAAGTTCGATCTATGGCTG+AGG | + | chr1.2:76505580-76505599 | MS.gene34391:CDS | 45.0% | |
| GTGGAATTAGCAAAAGGGCA+GGG | + | chr1.2:76506152-76506171 | MS.gene34391:CDS | 45.0% | |
| TCTACAACTGCGAGACGTTA+TGG | - | chr1.2:76505772-76505791 | None:intergenic | 45.0% | |
| TCTAGGCATGCTTGAAATGC+TGG | - | chr1.2:76505498-76505517 | None:intergenic | 45.0% | |
| TGGGGGTGGAATTAGCAAAA+GGG | + | chr1.2:76506147-76506166 | MS.gene34391:CDS | 45.0% | |
| TGTCCTCAAATGCAGAGCTT+TGG | + | chr1.2:76506300-76506319 | MS.gene34391:CDS | 45.0% | |
| ! | AATGGAAGGTTGGCCATGAT+TGG | + | chr1.2:76506110-76506129 | MS.gene34391:CDS | 45.0% |
| ! | TTTTGTATCTGCAATGGGGG+TGG | + | chr1.2:76506133-76506152 | MS.gene34391:CDS | 45.0% |
| !! | CGGATTTGATGGCTTTTGGT+GGG | + | chr1.2:76506069-76506088 | MS.gene34391:CDS | 45.0% |
| !! | GGATTTGATGGCTTTTGGTG+GGG | + | chr1.2:76506070-76506089 | MS.gene34391:CDS | 45.0% |
| !! | TCGGATTTGATGGCTTTTGG+TGG | + | chr1.2:76506068-76506087 | MS.gene34391:CDS | 45.0% |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.2:76505846-76505865 | MS.gene34391:CDS | 50.0% | |
| GCAGCACCTGAAAGGATCAA+TGG | + | chr1.2:76506092-76506111 | MS.gene34391:CDS | 50.0% | |
| GCGAGACGTTATGGATTCTC+CGG | - | chr1.2:76505763-76505782 | None:intergenic | 50.0% | |
| GGTGGAATTAGCAAAAGGGC+AGG | + | chr1.2:76506151-76506170 | MS.gene34391:CDS | 50.0% | |
| !! | TATCTGGTGGTGGAATCCCA+TGG | + | chr1.2:76506189-76506208 | MS.gene34391:CDS | 50.0% |
| GGTGAACGAGTAGTAGAGGC+AGG | - | chr1.2:76505883-76505902 | None:intergenic | 55.0% | |
| CGAGTAGTAGAGGCAGGCTG+TGG | - | chr1.2:76505877-76505896 | None:intergenic | 60.0% | |
| TTGGTGGGGCAGCACCTGAA+AGG | + | chr1.2:76506084-76506103 | MS.gene34391:CDS | 60.0% | |
| !! | GCAGGCTGTGGTGTCACCTT+TGG | - | chr1.2:76505865-76505884 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 76505452 | 76506397 | 76505452 | ID=MS.gene34391 |
| chr1.2 | mRNA | 76505452 | 76506397 | 76505452 | ID=MS.gene34391.t1;Parent=MS.gene34391 |
| chr1.2 | exon | 76505452 | 76505601 | 76505452 | ID=MS.gene34391.t1.exon1;Parent=MS.gene34391.t1 |
| chr1.2 | CDS | 76505452 | 76505601 | 76505452 | ID=cds.MS.gene34391.t1;Parent=MS.gene34391.t1 |
| chr1.2 | exon | 76505844 | 76505906 | 76505844 | ID=MS.gene34391.t1.exon2;Parent=MS.gene34391.t1 |
| chr1.2 | CDS | 76505844 | 76505906 | 76505844 | ID=cds.MS.gene34391.t1;Parent=MS.gene34391.t1 |
| chr1.2 | exon | 76506053 | 76506397 | 76506053 | ID=MS.gene34391.t1.exon3;Parent=MS.gene34391.t1 |
| chr1.2 | CDS | 76506053 | 76506397 | 76506053 | ID=cds.MS.gene34391.t1;Parent=MS.gene34391.t1 |
| Gene Sequence |
| Protein sequence |
