Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34392.t1 | RHN81944.1 | 84.8 | 198 | 30 | 0 | 1 | 198 | 1 | 198 | 1.20E-81 | 312.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34392.t1 | P11432 | 76.3 | 198 | 45 | 2 | 1 | 198 | 1 | 196 | 7.2e-72 | 271.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34392.t1 | A0A396JXB1 | 84.8 | 198 | 30 | 0 | 1 | 198 | 1 | 198 | 8.4e-82 | 312.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05081 | MS.gene34392 | 0.820385 | 7.06E-53 | -1.69E-46 |
| MS.gene051734 | MS.gene34392 | 0.817476 | 3.23E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene34392 | 0.808848 | 2.53E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene34392 | 0.821631 | 3.64E-53 | -1.69E-46 |
| MS.gene052870 | MS.gene34392 | 0.840036 | 1.11E-57 | -1.69E-46 |
| MS.gene052872 | MS.gene34392 | 0.84425 | 8.50E-59 | -1.69E-46 |
| MS.gene053824 | MS.gene34392 | 0.820004 | 8.62E-53 | -1.69E-46 |
| MS.gene054344 | MS.gene34392 | 0.811645 | 6.31E-51 | -1.69E-46 |
| MS.gene054884 | MS.gene34392 | -0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene34392 | 0.813886 | 2.04E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene34392 | 0.810651 | 1.04E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene34392 | 0.813603 | 2.35E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene34392 | 0.813641 | 2.31E-51 | -1.69E-46 |
| MS.gene058444 | MS.gene34392 | 0.801231 | 9.93E-49 | -1.69E-46 |
| MS.gene059717 | MS.gene34392 | 0.807663 | 4.53E-50 | -1.69E-46 |
| MS.gene061231 | MS.gene34392 | 0.830577 | 2.72E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34392.t1 | MTR_1g102800 | 91.011 | 178 | 16 | 0 | 21 | 198 | 20 | 197 | 3.60e-110 | 313 |
| MS.gene34392.t1 | MTR_1g102570 | 89.899 | 198 | 20 | 0 | 1 | 198 | 1 | 198 | 8.76e-109 | 310 |
| MS.gene34392.t1 | MTR_1g102780 | 91.000 | 200 | 16 | 1 | 1 | 198 | 1 | 200 | 8.45e-104 | 297 |
| MS.gene34392.t1 | MTR_1g102830 | 78.894 | 199 | 28 | 2 | 1 | 198 | 1 | 186 | 2.76e-96 | 278 |
| MS.gene34392.t1 | MTR_1g102810 | 76.374 | 182 | 29 | 2 | 18 | 198 | 14 | 182 | 3.10e-96 | 277 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34392.t1 | AT3G22840 | 58.896 | 163 | 67 | 0 | 36 | 198 | 33 | 195 | 3.52e-61 | 189 |
| MS.gene34392.t1 | AT4G14690 | 58.642 | 162 | 66 | 1 | 38 | 198 | 32 | 193 | 1.24e-59 | 185 |
Find 49 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGGAGTCCCATGGTTCT+TGG | 0.283070 | 1.2:+76501442 | MS.gene34392:CDS |
| GGTGGAGTCCCATGGTTCTT+GGG | 0.285032 | 1.2:+76501443 | MS.gene34392:CDS |
| GGTGATGATGATGGTGTAAT+TGG | 0.299898 | 1.2:-76501104 | None:intergenic |
| CCAGCACCTGAGAGAATTAA+TGG | 0.300603 | 1.2:+76501338 | MS.gene34392:CDS |
| GGTTTGTTTGATCAAATATC+TGG | 0.314167 | 1.2:+76501419 | MS.gene34392:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.357312 | 1.2:+76501583 | MS.gene34392:CDS |
| ATTGGGTCTACAGGCACCTT+TGG | 0.360861 | 1.2:-76501086 | None:intergenic |
| AATGGAAGGTTGGCTATGAT+TGG | 0.383969 | 1.2:+76501356 | MS.gene34392:CDS |
| CCATTAATTCTCTCAGGTGC+TGG | 0.425775 | 1.2:-76501338 | None:intergenic |
| GCTGGTTGTGGTGTTGGTGT+TGG | 0.432144 | 1.2:-76501125 | None:intergenic |
| GAATGGAAGAATAGCTATGT+TGG | 0.436992 | 1.2:+76501568 | MS.gene34392:CDS |
| AGCTCTAGCCCTGCTAGAAA+TGG | 0.446558 | 1.2:-76500812 | None:intergenic |
| AACCTTCCATTAATTCTCTC+AGG | 0.456054 | 1.2:-76501344 | None:intergenic |
| AAGCTAGGCATGTACACAAA+AGG | 0.459561 | 1.2:-76500853 | None:intergenic |
| GTGATGATGATGGTGTAATT+GGG | 0.461935 | 1.2:-76501103 | None:intergenic |
| CATTGAGCACCATTTCTAGC+AGG | 0.478291 | 1.2:+76500803 | MS.gene34392:CDS |
| TGTCCTCAGATGCTGAGTTA+TGG | 0.479277 | 1.2:+76501546 | MS.gene34392:CDS |
| GTTGGTGTTGGTGATGATGA+TGG | 0.479914 | 1.2:-76501113 | None:intergenic |
| GTGGAGTCCCATGGTTCTTG+GGG | 0.481099 | 1.2:+76501444 | MS.gene34392:CDS |
| AATGGAAGAATAGCTATGTT+GGG | 0.501527 | 1.2:+76501569 | MS.gene34392:CDS |
| GTGTAGGCTGGTTGTGGTGT+TGG | 0.503331 | 1.2:-76501131 | None:intergenic |
| AGTGTGGAGTCTAAGTCAAA+AGG | 0.525000 | 1.2:+76501518 | MS.gene34392:CDS |
| AACCTTATTTACCTCAGCCA+TGG | 0.525798 | 1.2:-76500905 | None:intergenic |
| CTAGCATTCCTTCTGAAGCT+AGG | 0.532093 | 1.2:-76500868 | None:intergenic |
| AGCTAGGCATGTACACAAAA+GGG | 0.534196 | 1.2:-76500852 | None:intergenic |
| TTTGGTGAGCGAGTGTAGGC+TGG | 0.536506 | 1.2:-76501143 | None:intergenic |
| CACTTGTCCCCAAGAACCAT+GGG | 0.536973 | 1.2:-76501451 | None:intergenic |
| GGTGGAAATAGCAAATGGAC+AGG | 0.544893 | 1.2:+76501397 | MS.gene34392:CDS |
| GAGCGAGTGTAGGCTGGTTG+TGG | 0.550985 | 1.2:-76501137 | None:intergenic |
| TTTGATCAAATATCTGGTGG+TGG | 0.551757 | 1.2:+76501425 | MS.gene34392:CDS |
| TGAGAGAATTAATGGAAGGT+TGG | 0.551924 | 1.2:+76501346 | MS.gene34392:CDS |
| GATGGTGTAATTGGGTCTAC+AGG | 0.568013 | 1.2:-76501095 | None:intergenic |
| TCAGATGCTGAGTTATGGAA+TGG | 0.568279 | 1.2:+76501551 | MS.gene34392:CDS |
| TTGTTTGATCAAATATCTGG+TGG | 0.570609 | 1.2:+76501422 | MS.gene34392:CDS |
| TGTACATGCCTAGCTTCAGA+AGG | 0.587219 | 1.2:+76500860 | MS.gene34392:CDS |
| ATTCCATAACTCAGCATCTG+AGG | 0.591329 | 1.2:-76501549 | None:intergenic |
| CTATAGAACTCTATTACACA+AGG | 0.596246 | 1.2:-76501634 | None:intergenic |
| TAGACTCCACACTAACACCT+TGG | 0.597870 | 1.2:-76501508 | None:intergenic |
| ATTGAGCACCATTTCTAGCA+GGG | 0.604034 | 1.2:+76500804 | MS.gene34392:CDS |
| TAGCTTGAAAGTTCGATCCA+TGG | 0.604578 | 1.2:+76500888 | MS.gene34392:CDS |
| ACACTTGTCCCCAAGAACCA+TGG | 0.606138 | 1.2:-76501452 | None:intergenic |
| AGAGCAGAAAGAGCAACCAA+AGG | 0.611218 | 1.2:+76501070 | MS.gene34392:CDS |
| AGCCTACACTCGCTCACCAA+AGG | 0.611565 | 1.2:+76501145 | MS.gene34392:CDS |
| GAAAGTTCGATCCATGGCTG+AGG | 0.622095 | 1.2:+76500894 | MS.gene34392:CDS |
| CACCTGAGAGAATTAATGGA+AGG | 0.651384 | 1.2:+76501342 | MS.gene34392:CDS |
| TATCTGGTGGTGGAGTCCCA+TGG | 0.653372 | 1.2:+76501435 | MS.gene34392:CDS |
| TACCTTTGGTGAGCGAGTGT+AGG | 0.653559 | 1.2:-76501147 | None:intergenic |
| GTGGAAATAGCAAATGGACA+GGG | 0.661963 | 1.2:+76501398 | MS.gene34392:CDS |
| TATAGAACTCTATTACACAA+GGG | 0.699610 | 1.2:-76501633 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAATGATGAAAATA+TGG | + | chr1.2:76501273-76501292 | MS.gene34392:intron | 10.0% |
| !! | ATAATATGTTTATGATTTAT+TGG | + | chr1.2:76501022-76501041 | MS.gene34392:intron | 10.0% |
| !! | CATCATTATTATTTATTAAA+AGG | - | chr1.2:76501267-76501286 | None:intergenic | 10.0% |
| !! | TATAAGATAAACAAAATTTA+AGG | + | chr1.2:76500928-76500947 | MS.gene34392:intron | 10.0% |
| !! | AAATCATAAACATATTATGT+TGG | - | chr1.2:76501020-76501039 | None:intergenic | 15.0% |
| !!! | ATTTTGCTAAGTACTAAATT+AGG | + | chr1.2:76501226-76501245 | MS.gene34392:intron | 20.0% |
| AATGGAAGAATAGCTATGTT+GGG | + | chr1.2:76501569-76501588 | MS.gene34392:CDS | 30.0% | |
| TTGTTTGATCAAATATCTGG+TGG | + | chr1.2:76501422-76501441 | MS.gene34392:CDS | 30.0% | |
| TTTGTTACTATGCATACCTT+TGG | - | chr1.2:76501164-76501183 | None:intergenic | 30.0% | |
| ! | GGTTTGTTTGATCAAATATC+TGG | + | chr1.2:76501419-76501438 | MS.gene34392:CDS | 30.0% |
| !! | TATTATGTTGGTGATGATGA+AGG | - | chr1.2:76501008-76501027 | None:intergenic | 30.0% |
| !! | TCTTTGATTCCATTTTTCCA+AGG | + | chr1.2:76501491-76501510 | MS.gene34392:CDS | 30.0% |
| AACCTTCCATTAATTCTCTC+AGG | - | chr1.2:76501347-76501366 | None:intergenic | 35.0% | |
| ACACTAACACCTTGGAAAAA+TGG | - | chr1.2:76501503-76501522 | None:intergenic | 35.0% | |
| GAATGGAAGAATAGCTATGT+TGG | + | chr1.2:76501568-76501587 | MS.gene34392:CDS | 35.0% | |
| GATTGCGTGTTACATTATTC+AGG | + | chr1.2:76501046-76501065 | MS.gene34392:intron | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.2:76501583-76501602 | MS.gene34392:CDS | 35.0% | |
| TGAGAGAATTAATGGAAGGT+TGG | + | chr1.2:76501346-76501365 | MS.gene34392:CDS | 35.0% | |
| TTTGATCAAATATCTGGTGG+TGG | + | chr1.2:76501425-76501444 | MS.gene34392:CDS | 35.0% | |
| ! | CACAAAGTTTTCGGATTTGA+TGG | + | chr1.2:76501304-76501323 | MS.gene34392:CDS | 35.0% |
| ! | GCTTTTACAGAGTACGTTAA+GGG | + | chr1.2:76501605-76501624 | MS.gene34392:CDS | 35.0% |
| !! | ATTTTTCCAAGGTGTTAGTG+TGG | + | chr1.2:76501502-76501521 | MS.gene34392:CDS | 35.0% |
| !! | GTGATGATGATGGTGTAATT+GGG | - | chr1.2:76501106-76501125 | None:intergenic | 35.0% |
| !! | TTTTCGGATTTGATGGCTTT+TGG | + | chr1.2:76501311-76501330 | MS.gene34392:CDS | 35.0% |
| !!! | ATTGGTTTTGTAGCTGCAAT+GGG | + | chr1.2:76501374-76501393 | MS.gene34392:CDS | 35.0% |
| AAGCTAGGCATGTACACAAA+AGG | - | chr1.2:76500856-76500875 | None:intergenic | 40.0% | |
| AGCTAGGCATGTACACAAAA+GGG | - | chr1.2:76500855-76500874 | None:intergenic | 40.0% | |
| AGTGTGGAGTCTAAGTCAAA+AGG | + | chr1.2:76501518-76501537 | MS.gene34392:CDS | 40.0% | |
| ATCCATGGCTGAGGTAAATA+AGG | + | chr1.2:76500903-76500922 | MS.gene34392:intron | 40.0% | |
| ATTCCATAACTCAGCATCTG+AGG | - | chr1.2:76501552-76501571 | None:intergenic | 40.0% | |
| CACCTGAGAGAATTAATGGA+AGG | + | chr1.2:76501342-76501361 | MS.gene34392:CDS | 40.0% | |
| GTGGAAATAGCAAATGGACA+GGG | + | chr1.2:76501398-76501417 | MS.gene34392:CDS | 40.0% | |
| TAGCTTGAAAGTTCGATCCA+TGG | + | chr1.2:76500888-76500907 | MS.gene34392:CDS | 40.0% | |
| TCAGATGCTGAGTTATGGAA+TGG | + | chr1.2:76501551-76501570 | MS.gene34392:CDS | 40.0% | |
| ! | AACCTTATTTACCTCAGCCA+TGG | - | chr1.2:76500908-76500927 | None:intergenic | 40.0% |
| ! | AATGGAAGGTTGGCTATGAT+TGG | + | chr1.2:76501356-76501375 | MS.gene34392:CDS | 40.0% |
| ! | GCAGATGAGCACAAAGTTTT+CGG | + | chr1.2:76501295-76501314 | MS.gene34392:intron | 40.0% |
| ! | GGCTTTTACAGAGTACGTTA+AGG | + | chr1.2:76501604-76501623 | MS.gene34392:CDS | 40.0% |
| ! | GGTGATGATGAAGGTGTAAT+CGG | - | chr1.2:76500999-76501018 | None:intergenic | 40.0% |
| !! | ATTGAGCACCATTTCTAGCA+GGG | + | chr1.2:76500804-76500823 | MS.gene34392:CDS | 40.0% |
| !! | GGTGATGATGATGGTGTAAT+TGG | - | chr1.2:76501107-76501126 | None:intergenic | 40.0% |
| !!! | GATTGGTTTTGTAGCTGCAA+TGG | + | chr1.2:76501373-76501392 | MS.gene34392:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCTGCAATG+GGG | + | chr1.2:76501375-76501394 | MS.gene34392:CDS | 40.0% |
| AGAGCAGAAAGAGCAACCAA+AGG | + | chr1.2:76501070-76501089 | MS.gene34392:CDS | 45.0% | |
| ATGGGGGTGGAAATAGCAAA+TGG | + | chr1.2:76501392-76501411 | MS.gene34392:CDS | 45.0% | |
| CCAGCACCTGAGAGAATTAA+TGG | + | chr1.2:76501338-76501357 | MS.gene34392:CDS | 45.0% | |
| CCATTAATTCTCTCAGGTGC+TGG | - | chr1.2:76501341-76501360 | None:intergenic | 45.0% | |
| GATGGTGTAATTGGGTCTAC+AGG | - | chr1.2:76501098-76501117 | None:intergenic | 45.0% | |
| GGTGGAAATAGCAAATGGAC+AGG | + | chr1.2:76501397-76501416 | MS.gene34392:CDS | 45.0% | |
| TAGACTCCACACTAACACCT+TGG | - | chr1.2:76501511-76501530 | None:intergenic | 45.0% | |
| TGTACATGCCTAGCTTCAGA+AGG | + | chr1.2:76500860-76500879 | MS.gene34392:CDS | 45.0% | |
| TGTCCTCAGATGCTGAGTTA+TGG | + | chr1.2:76501546-76501565 | MS.gene34392:CDS | 45.0% | |
| !! | CATTGAGCACCATTTCTAGC+AGG | + | chr1.2:76500803-76500822 | MS.gene34392:CDS | 45.0% |
| !! | CGGATTTGATGGCTTTTGGT+GGG | + | chr1.2:76501315-76501334 | MS.gene34392:CDS | 45.0% |
| !! | CTAGCATTCCTTCTGAAGCT+AGG | - | chr1.2:76500871-76500890 | None:intergenic | 45.0% |
| !! | GTTGGTGTTGGTGATGATGA+TGG | - | chr1.2:76501116-76501135 | None:intergenic | 45.0% |
| !! | TCGGATTTGATGGCTTTTGG+TGG | + | chr1.2:76501314-76501333 | MS.gene34392:CDS | 45.0% |
| !!! | TGGTTTTGTAGCTGCAATGG+GGG | + | chr1.2:76501376-76501395 | MS.gene34392:CDS | 45.0% |
| ACACTTGTCCCCAAGAACCA+TGG | - | chr1.2:76501455-76501474 | None:intergenic | 50.0% | |
| ATTGGGTCTACAGGCACCTT+TGG | - | chr1.2:76501089-76501108 | None:intergenic | 50.0% | |
| CACTTGTCCCCAAGAACCAT+GGG | - | chr1.2:76501454-76501473 | None:intergenic | 50.0% | |
| GAAAGTTCGATCCATGGCTG+AGG | + | chr1.2:76500894-76500913 | MS.gene34392:CDS | 50.0% | |
| TACCTTTGGTGAGCGAGTGT+AGG | - | chr1.2:76501150-76501169 | None:intergenic | 50.0% | |
| ! | AGCTCTAGCCCTGCTAGAAA+TGG | - | chr1.2:76500815-76500834 | None:intergenic | 50.0% |
| ! | TTTTGTAGCTGCAATGGGGG+TGG | + | chr1.2:76501379-76501398 | MS.gene34392:CDS | 50.0% |
| AGCCTACACTCGCTCACCAA+AGG | + | chr1.2:76501145-76501164 | MS.gene34392:CDS | 55.0% | |
| GGTGGAGTCCCATGGTTCTT+GGG | + | chr1.2:76501443-76501462 | MS.gene34392:CDS | 55.0% | |
| GTGGAGTCCCATGGTTCTTG+GGG | + | chr1.2:76501444-76501463 | MS.gene34392:CDS | 55.0% | |
| TGGTGGAGTCCCATGGTTCT+TGG | + | chr1.2:76501442-76501461 | MS.gene34392:CDS | 55.0% | |
| TTTGGTGAGCGAGTGTAGGC+TGG | - | chr1.2:76501146-76501165 | None:intergenic | 55.0% | |
| ! | GTGTAGGCTGGTTGTGGTGT+TGG | - | chr1.2:76501134-76501153 | None:intergenic | 55.0% |
| !! | GCTGGTTGTGGTGTTGGTGT+TGG | - | chr1.2:76501128-76501147 | None:intergenic | 55.0% |
| !! | TATCTGGTGGTGGAGTCCCA+TGG | + | chr1.2:76501435-76501454 | MS.gene34392:CDS | 55.0% |
| ! | GAGCGAGTGTAGGCTGGTTG+TGG | - | chr1.2:76501140-76501159 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 76500763 | 76501643 | 76500763 | ID=MS.gene34392 |
| chr1.2 | mRNA | 76500763 | 76501643 | 76500763 | ID=MS.gene34392.t1;Parent=MS.gene34392 |
| chr1.2 | exon | 76500763 | 76500915 | 76500763 | ID=MS.gene34392.t1.exon1;Parent=MS.gene34392.t1 |
| chr1.2 | CDS | 76500763 | 76500915 | 76500763 | ID=cds.MS.gene34392.t1;Parent=MS.gene34392.t1 |
| chr1.2 | exon | 76501068 | 76501166 | 76501068 | ID=MS.gene34392.t1.exon2;Parent=MS.gene34392.t1 |
| chr1.2 | CDS | 76501068 | 76501166 | 76501068 | ID=cds.MS.gene34392.t1;Parent=MS.gene34392.t1 |
| chr1.2 | exon | 76501299 | 76501643 | 76501299 | ID=MS.gene34392.t1.exon3;Parent=MS.gene34392.t1 |
| chr1.2 | CDS | 76501299 | 76501643 | 76501299 | ID=cds.MS.gene34392.t1;Parent=MS.gene34392.t1 |
| Gene Sequence |
| Protein sequence |
