Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34947.t1 | RHN42362.1 | 98.7 | 384 | 2 | 1 | 1 | 381 | 1 | 384 | 4.30E-218 | 766.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34947.t1 | A0A396GMP1 | 98.7 | 384 | 2 | 1 | 1 | 381 | 1 | 384 | 3.1e-218 | 766.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049801 | MS.gene34947 | 0.817854 | 2.66E-52 | -1.69E-46 |
| MS.gene053079 | MS.gene34947 | 0.800122 | 1.67E-48 | -1.69E-46 |
| MS.gene053575 | MS.gene34947 | 0.800473 | 1.42E-48 | -1.69E-46 |
| MS.gene05761 | MS.gene34947 | 0.82604 | 3.37E-54 | -1.69E-46 |
| MS.gene057869 | MS.gene34947 | 0.810149 | 1.33E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene34947 | 0.803913 | 2.78E-49 | -1.69E-46 |
| MS.gene058919 | MS.gene34947 | 0.804976 | 1.67E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene34947 | 0.800792 | 1.22E-48 | -1.69E-46 |
| MS.gene060998 | MS.gene34947 | 0.813786 | 2.15E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34947.t1 | MTR_6g082450 | 70.149 | 402 | 98 | 4 | 1 | 381 | 1 | 401 | 0.0 | 553 |
| MS.gene34947.t1 | MTR_1g110070 | 50.971 | 412 | 157 | 10 | 1 | 373 | 1 | 406 | 1.43e-123 | 363 |
| MS.gene34947.t1 | MTR_4g120990 | 44.301 | 386 | 190 | 9 | 10 | 381 | 21 | 395 | 2.50e-97 | 295 |
| MS.gene34947.t1 | MTR_2g035080 | 44.380 | 347 | 170 | 6 | 43 | 381 | 70 | 401 | 5.80e-89 | 274 |
| MS.gene34947.t1 | MTR_4g044297 | 38.024 | 334 | 161 | 7 | 48 | 373 | 5 | 300 | 5.16e-65 | 209 |
| MS.gene34947.t1 | MTR_2g090210 | 40.483 | 331 | 157 | 11 | 51 | 373 | 10 | 308 | 4.37e-63 | 204 |
| MS.gene34947.t1 | MTR_6g066280 | 33.133 | 332 | 168 | 6 | 77 | 375 | 15 | 325 | 1.42e-49 | 170 |
| MS.gene34947.t1 | MTR_1g077460 | 33.623 | 345 | 187 | 9 | 52 | 373 | 13 | 338 | 7.67e-43 | 152 |
| MS.gene34947.t1 | MTR_1g087810 | 32.268 | 313 | 141 | 7 | 66 | 377 | 70 | 312 | 8.49e-39 | 141 |
| MS.gene34947.t1 | MTR_1g087830 | 38.919 | 185 | 100 | 4 | 194 | 377 | 131 | 303 | 4.18e-34 | 128 |
| MS.gene34947.t1 | MTR_1g087830 | 62.222 | 45 | 17 | 0 | 87 | 131 | 88 | 132 | 1.26e-12 | 68.6 |
| MS.gene34947.t1 | MTR_6g077930 | 31.527 | 203 | 94 | 6 | 210 | 379 | 917 | 1107 | 1.05e-16 | 82.4 |
| MS.gene34947.t1 | MTR_6g044130 | 32.308 | 130 | 70 | 4 | 252 | 363 | 1 | 130 | 2.97e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34947.t1 | AT1G15030 | 48.942 | 378 | 167 | 12 | 1 | 373 | 1 | 357 | 1.39e-99 | 300 |
| MS.gene34947.t1 | AT2G01260 | 45.897 | 390 | 170 | 12 | 1 | 373 | 1 | 366 | 6.87e-96 | 291 |
| MS.gene34947.t1 | AT4G16100 | 40.194 | 413 | 184 | 8 | 2 | 376 | 1 | 388 | 3.34e-91 | 280 |
| MS.gene34947.t1 | AT5G49220 | 45.646 | 333 | 139 | 7 | 51 | 376 | 107 | 404 | 5.36e-85 | 264 |
| MS.gene34947.t1 | AT1G15030 | 48.684 | 304 | 133 | 10 | 1 | 300 | 1 | 285 | 2.01e-74 | 233 |
| MS.gene34947.t1 | AT2G01260 | 45.373 | 335 | 140 | 12 | 1 | 318 | 1 | 309 | 2.92e-73 | 231 |
| MS.gene34947.t1 | AT1G03610 | 39.144 | 327 | 157 | 7 | 51 | 373 | 11 | 299 | 2.91e-63 | 205 |
| MS.gene34947.t1 | AT4G03420 | 38.323 | 334 | 158 | 8 | 51 | 373 | 7 | 303 | 8.83e-63 | 204 |
| MS.gene34947.t1 | AT4G28150 | 36.196 | 326 | 148 | 7 | 46 | 367 | 3 | 272 | 2.27e-55 | 184 |
| MS.gene34947.t1 | AT4G28150 | 36.111 | 324 | 149 | 7 | 46 | 367 | 3 | 270 | 7.58e-54 | 180 |
| MS.gene34947.t1 | AT1G03610 | 41.313 | 259 | 103 | 6 | 51 | 301 | 11 | 228 | 1.52e-51 | 172 |
| MS.gene34947.t1 | AT1G17830 | 36.095 | 338 | 177 | 8 | 51 | 373 | 18 | 331 | 3.26e-50 | 172 |
| MS.gene34947.t1 | AT1G73210 | 36.890 | 328 | 172 | 10 | 51 | 373 | 12 | 309 | 2.62e-47 | 164 |
| MS.gene34947.t1 | AT1G73210 | 36.890 | 328 | 172 | 10 | 51 | 373 | 12 | 309 | 2.62e-47 | 164 |
| MS.gene34947.t1 | AT1G73210 | 38.014 | 292 | 153 | 8 | 87 | 373 | 39 | 307 | 8.95e-47 | 162 |
| MS.gene34947.t1 | AT2G01260 | 36.299 | 281 | 130 | 12 | 1 | 264 | 1 | 249 | 1.06e-34 | 129 |
| MS.gene34947.t1 | AT4G28150 | 37.281 | 228 | 100 | 5 | 46 | 269 | 3 | 191 | 1.93e-33 | 124 |
| MS.gene34947.t1 | AT5G23380 | 32.164 | 342 | 168 | 11 | 51 | 379 | 7 | 297 | 3.49e-31 | 120 |
| MS.gene34947.t1 | AT5G23380 | 33.206 | 262 | 117 | 9 | 51 | 301 | 7 | 221 | 8.31e-23 | 95.9 |
| MS.gene34947.t1 | AT5G23380 | 33.206 | 262 | 117 | 9 | 51 | 301 | 7 | 221 | 8.31e-23 | 95.9 |
| MS.gene34947.t1 | AT5G08360 | 31.677 | 161 | 93 | 4 | 216 | 375 | 22 | 166 | 5.75e-16 | 75.9 |
| MS.gene34947.t1 | AT5G08360 | 31.579 | 133 | 75 | 3 | 243 | 375 | 5 | 121 | 1.23e-14 | 70.9 |
Find 86 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAGATCCCTTTAATTTAT+AGG | 0.179141 | 4.4:+17625154 | None:intergenic |
| ATAAATTAAAGGGATCTATT+TGG | 0.209391 | 4.4:-17625151 | MS.gene34947:CDS |
| CTTGGATTCCACAAAGTTAT+TGG | 0.224982 | 4.4:-17634337 | MS.gene34947:CDS |
| ACTTTGCTCTGAATGATCTA+TGG | 0.228571 | 4.4:-17633568 | MS.gene34947:CDS |
| AATGGCTGCCTATAAATTAA+AGG | 0.234653 | 4.4:-17625162 | MS.gene34947:CDS |
| GGAAGCAGTGATTGCGAATA+TGG | 0.267161 | 4.4:-17629555 | MS.gene34947:CDS |
| TGATCTATGGGAATCGTTTA+AGG | 0.293061 | 4.4:-17633555 | MS.gene34947:CDS |
| AGTAGTGATGACAGTGAAAC+TGG | 0.302541 | 4.4:-17629411 | MS.gene34947:CDS |
| TTTAAGGAGTGGAGTGCTTA+TGG | 0.330153 | 4.4:-17633539 | MS.gene34947:CDS |
| AAAGTAGGGATGGAAATATT+TGG | 0.332123 | 4.4:+17625188 | None:intergenic |
| CTGGGTACTGCACTGCAATT+TGG | 0.335190 | 4.4:-17634501 | MS.gene34947:CDS |
| GTGATTGCGAATATGGAAAA+AGG | 0.338454 | 4.4:-17629548 | MS.gene34947:CDS |
| TCGTGCAGGCTGCAGATAAA+TGG | 0.347650 | 4.4:-17625088 | MS.gene34947:CDS |
| TTGGAACAAGGTCATCCTTA+TGG | 0.360520 | 4.4:-17629360 | MS.gene34947:CDS |
| TAGTAACATTGATAGGTTCT+TGG | 0.367105 | 4.4:-17634355 | MS.gene34947:CDS |
| TTGTCGGCTATTCAGCTATA+TGG | 0.370596 | 4.4:-17633455 | MS.gene34947:CDS |
| CCCTTTGATCAAGTAGTAAA+GGG | 0.386917 | 4.4:+17633507 | None:intergenic |
| AGTCTGTTAGTAACATTGAT+AGG | 0.410081 | 4.4:-17634362 | MS.gene34947:CDS |
| GTACTATGTTCCGTATTTGT+CGG | 0.410526 | 4.4:-17633471 | MS.gene34947:CDS |
| TCCTACTCCTGGTCCTGTAC+TGG | 0.411890 | 4.4:-17625237 | MS.gene34947:CDS |
| CCTTGTCAGCCAGCGGTTCA+CGG | 0.419823 | 4.4:+17629337 | None:intergenic |
| CCCTTTACTACTTGATCAAA+GGG | 0.420560 | 4.4:-17633507 | MS.gene34947:CDS |
| TAAATGGTTAAGGCTAGTTC+AGG | 0.422552 | 4.4:-17625072 | MS.gene34947:CDS |
| TCCTTATGGCCGTGAACCGC+TGG | 0.427561 | 4.4:-17629346 | MS.gene34947:CDS |
| ATGGCTGCCTATAAATTAAA+GGG | 0.433226 | 4.4:-17625161 | MS.gene34947:CDS |
| AATTACCTGTCAAGGGTGTA+TGG | 0.444633 | 4.4:+17628947 | None:intergenic |
| GAGAAGAGACACCACTTGTC+AGG | 0.454698 | 4.4:+17629490 | None:intergenic |
| TGATTCTTTCTTGTCTTCAC+CGG | 0.457385 | 4.4:+17634456 | None:intergenic |
| TCCCTTTGATCAAGTAGTAA+AGG | 0.457484 | 4.4:+17633506 | None:intergenic |
| GCAACAGACATCCAACTGTT+TGG | 0.468240 | 4.4:+17629172 | None:intergenic |
| CTTTGCTCTGAATGATCTAT+GGG | 0.469746 | 4.4:-17633567 | MS.gene34947:CDS |
| TAGGCAGCCATTGCAAAAGT+AGG | 0.473321 | 4.4:+17625173 | None:intergenic |
| GATTTAAAGAACTGATGATC+CGG | 0.473580 | 4.4:+17625041 | None:intergenic |
| TGCAATTTGGTGGTGATGAT+CGG | 0.482447 | 4.4:-17634488 | MS.gene34947:CDS |
| CCGTGAACCGCTGGCTGACA+AGG | 0.488317 | 4.4:-17629337 | MS.gene34947:intron |
| CCTGTGGGTATTCGATATAT+AGG | 0.490923 | 4.4:+17629018 | None:intergenic |
| GCAGGAAGTGGTCCTACTCC+TGG | 0.503247 | 4.4:-17625248 | MS.gene34947:intron |
| TCCCTTTACTACTTGATCAA+AGG | 0.504346 | 4.4:-17633508 | MS.gene34947:CDS |
| AGCTGAATAGCCGACAAATA+CGG | 0.507690 | 4.4:+17633461 | None:intergenic |
| GAGTGGAGTGCTTATGGTGC+TGG | 0.509550 | 4.4:-17633533 | MS.gene34947:CDS |
| CATCAGTTCTTTAAATCGCA+TGG | 0.513728 | 4.4:-17625035 | MS.gene34947:CDS |
| GCCAGCGGTTCACGGCCATA+AGG | 0.520857 | 4.4:+17629345 | None:intergenic |
| TGGTACACCATCCATCTCAC+TGG | 0.523799 | 4.4:+17625208 | None:intergenic |
| AATTTCATAATTACCTGTCA+AGG | 0.525386 | 4.4:+17628939 | None:intergenic |
| TCAAGGGTGTATGGAGTGTA+TGG | 0.527173 | 4.4:+17628956 | None:intergenic |
| GAGAGACAAGAATGTTGAAG+AGG | 0.528313 | 4.4:-17634412 | MS.gene34947:CDS |
| AGCTGGAACCAATAACTTTG+TGG | 0.532701 | 4.4:+17634329 | None:intergenic |
| AGGCTGCAGATAAATGGTTA+AGG | 0.534889 | 4.4:-17625082 | MS.gene34947:CDS |
| GGGTGTATGGAGTGTATGGT+AGG | 0.540087 | 4.4:+17628960 | None:intergenic |
| TCCAGCTCAGTATTTCTCCA+AGG | 0.541108 | 4.4:-17634313 | MS.gene34947:intron |
| ACAAATACGGAACATAGTAC+TGG | 0.541312 | 4.4:+17633474 | None:intergenic |
| ACACTCCATACACCCTTGAC+AGG | 0.545222 | 4.4:-17628952 | MS.gene34947:intron |
| GGTACTGCACTGCAATTTGG+TGG | 0.551407 | 4.4:-17634498 | MS.gene34947:CDS |
| ATTTCATAATTACCTGTCAA+GGG | 0.551433 | 4.4:+17628940 | None:intergenic |
| AGTAATGAATTCCCTCGTGC+AGG | 0.552196 | 4.4:-17625102 | MS.gene34947:CDS |
| TCACTGGGATAAACCAGTAC+AGG | 0.565356 | 4.4:+17625224 | None:intergenic |
| CACAACTTCTCAACGACATG+AGG | 0.566285 | 4.4:+17629203 | None:intergenic |
| GAAATGTCAATACCTTGGCT+TGG | 0.573993 | 4.4:+17633409 | None:intergenic |
| ACCTTGGAGAAATACTGAGC+TGG | 0.575271 | 4.4:+17634312 | None:intergenic |
| GTGATTTATTGCCAAACAGT+TGG | 0.575495 | 4.4:-17629183 | MS.gene34947:CDS |
| ATTTATCTGCAGCCTGCACG+AGG | 0.576191 | 4.4:+17625090 | None:intergenic |
| AGGCAGCCATTGCAAAAGTA+GGG | 0.576587 | 4.4:+17625174 | None:intergenic |
| CTTCTCAACGACATGAGGGC+AGG | 0.577536 | 4.4:+17629208 | None:intergenic |
| TACAGAGATTCATGTAGTGA+AGG | 0.580192 | 4.4:-17629576 | MS.gene34947:CDS |
| AGAAAGGATTGGAAAATGCT+GGG | 0.581921 | 4.4:-17634519 | None:intergenic |
| TGTAGACGACAATGAGAGGT+TGG | 0.584962 | 4.4:-17633619 | MS.gene34947:intron |
| GACATTGCCTTGTCAGCCAG+CGG | 0.600162 | 4.4:+17629330 | None:intergenic |
| AGCCATTGCAAAAGTAGGGA+TGG | 0.600948 | 4.4:+17625178 | None:intergenic |
| AAACACAATACAGTACAAGA+AGG | 0.603124 | 4.4:-17629441 | MS.gene34947:CDS |
| GGATAAACCAGTACAGGACC+AGG | 0.604698 | 4.4:+17625230 | None:intergenic |
| ATTTAAAGAACTGATGATCC+GGG | 0.606138 | 4.4:+17625042 | None:intergenic |
| TATGGGAATCGTTTAAGGAG+TGG | 0.610261 | 4.4:-17633550 | MS.gene34947:CDS |
| AGCAGTAAACACCTGACAAG+TGG | 0.611354 | 4.4:-17629501 | MS.gene34947:CDS |
| AAAAGTCGAGTGCCAAGCCA+AGG | 0.611438 | 4.4:-17633421 | MS.gene34947:intron |
| CCTATATATCGAATACCCAC+AGG | 0.618281 | 4.4:-17629018 | MS.gene34947:CDS |
| GTAGTGATGACAGTGAAACT+GGG | 0.640422 | 4.4:-17629410 | MS.gene34947:CDS |
| ACTGGTTTATCCCAGTGAGA+TGG | 0.640430 | 4.4:-17625219 | MS.gene34947:CDS |
| GCTAGTTCAGGTGAATCACC+CGG | 0.646489 | 4.4:-17625060 | MS.gene34947:CDS |
| TGTCTGTAGACGACAATGAG+AGG | 0.651713 | 4.4:-17633623 | MS.gene34947:intron |
| ACAACTTCTCAACGACATGA+GGG | 0.653539 | 4.4:+17629204 | None:intergenic |
| TACATAAGTGAAGATAGTGA+TGG | 0.653910 | 4.4:-17629606 | MS.gene34947:CDS |
| GGTACACCATCCATCTCACT+GGG | 0.664900 | 4.4:+17625209 | None:intergenic |
| ACCAGTACAGGACCAGGAGT+AGG | 0.669545 | 4.4:+17625236 | None:intergenic |
| ACAGTTGGATGTCTGTTGCA+TGG | 0.675268 | 4.4:-17629168 | MS.gene34947:intron |
| TTTATCTGCAGCCTGCACGA+GGG | 0.693098 | 4.4:+17625091 | None:intergenic |
| GTTTATCCCAGTGAGATGGA+TGG | 0.699701 | 4.4:-17625215 | MS.gene34947:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 17625019 | 17634526 | 17625019 | ID=MS.gene34947 |
| chr4.4 | mRNA | 17625019 | 17634526 | 17625019 | ID=MS.gene34947.t1;Parent=MS.gene34947 |
| chr4.4 | exon | 17634314 | 17634526 | 17634314 | ID=MS.gene34947.t1.exon1;Parent=MS.gene34947.t1 |
| chr4.4 | CDS | 17634314 | 17634526 | 17634314 | ID=cds.MS.gene34947.t1;Parent=MS.gene34947.t1 |
| chr4.4 | exon | 17633422 | 17633636 | 17633422 | ID=MS.gene34947.t1.exon2;Parent=MS.gene34947.t1 |
| chr4.4 | CDS | 17633422 | 17633636 | 17633422 | ID=cds.MS.gene34947.t1;Parent=MS.gene34947.t1 |
| chr4.4 | exon | 17629338 | 17629629 | 17629338 | ID=MS.gene34947.t1.exon3;Parent=MS.gene34947.t1 |
| chr4.4 | CDS | 17629338 | 17629629 | 17629338 | ID=cds.MS.gene34947.t1;Parent=MS.gene34947.t1 |
| chr4.4 | exon | 17629169 | 17629254 | 17629169 | ID=MS.gene34947.t1.exon4;Parent=MS.gene34947.t1 |
| chr4.4 | CDS | 17629169 | 17629254 | 17629169 | ID=cds.MS.gene34947.t1;Parent=MS.gene34947.t1 |
| chr4.4 | exon | 17628953 | 17629044 | 17628953 | ID=MS.gene34947.t1.exon5;Parent=MS.gene34947.t1 |
| chr4.4 | CDS | 17628953 | 17629044 | 17628953 | ID=cds.MS.gene34947.t1;Parent=MS.gene34947.t1 |
| chr4.4 | exon | 17625019 | 17625266 | 17625019 | ID=MS.gene34947.t1.exon6;Parent=MS.gene34947.t1 |
| chr4.4 | CDS | 17625019 | 17625266 | 17625019 | ID=cds.MS.gene34947.t1;Parent=MS.gene34947.t1 |
| Gene Sequence |
| Protein sequence |
