Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35410.t1 | XP_024633300.1 | 96.7 | 60 | 2 | 0 | 1 | 60 | 86 | 145 | 9.80E-24 | 118.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35410.t1 | A0A2Z6LY45 | 93.3 | 60 | 4 | 0 | 1 | 60 | 75 | 134 | 1.0e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050809 | MS.gene35410 | -0.80875 | 2.66E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene35410 | 0.806748 | 7.08E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene35410 | 0.807732 | 4.38E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene35410 | 0.819199 | 1.32E-52 | -1.69E-46 |
| MS.gene052870 | MS.gene35410 | 0.803339 | 3.65E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene35410 | 0.810043 | 1.40E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene35410 | 0.801743 | 7.80E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene35410 | -0.817778 | 2.76E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene35410 | 0.819305 | 1.25E-52 | -1.69E-46 |
| MS.gene059717 | MS.gene35410 | 0.813117 | 3.01E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35410.t1 | MTR_2g086480 | 90.000 | 60 | 6 | 0 | 1 | 60 | 86 | 145 | 2.33e-31 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTACGTACATCAGCAATA+AGG | 0.310452 | 2.1:+15643269 | None:intergenic |
| AAATCAAGGTCACAAAGATT+AGG | 0.365491 | 2.1:-15643250 | MS.gene35410:CDS |
| AGGTTAAGGTACCTGAAAAC+TGG | 0.375495 | 2.1:-15643387 | MS.gene35410:CDS |
| AATCAAGGTCACAAAGATTA+GGG | 0.392972 | 2.1:-15643249 | MS.gene35410:CDS |
| TTTACGTACATCAGCAATAA+GGG | 0.413998 | 2.1:+15643270 | None:intergenic |
| AGAGATGAAGGGAAAGGTTA+AGG | 0.420194 | 2.1:-15643401 | None:intergenic |
| AGGAACAAAAGCTGAAAGAA+TGG | 0.465707 | 2.1:-15643360 | MS.gene35410:CDS |
| GGAACAAAAGCTGAAAGAAT+GGG | 0.483468 | 2.1:-15643359 | MS.gene35410:CDS |
| TTGCTGATGTACGTAAATCA+AGG | 0.545508 | 2.1:-15643264 | MS.gene35410:CDS |
| TAGGGAAGAGATGAAGGGAA+AGG | 0.571304 | 2.1:-15643407 | None:intergenic |
| GGTTAAGGTACCTGAAAACT+GGG | 0.585046 | 2.1:-15643386 | MS.gene35410:CDS |
| AGGCATAGGGAAGAGATGAA+GGG | 0.595639 | 2.1:-15643412 | None:intergenic |
| TCAAAATAAGAAACTAGCTG+TGG | 0.599837 | 2.1:+15643224 | None:intergenic |
| GGTACCTGAAAACTGGGAGA+AGG | 0.618394 | 2.1:-15643380 | MS.gene35410:CDS |
| GCAGTAACAATCAATGCATG+AGG | 0.659977 | 2.1:+15643301 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCAAGGTCACAAAGATT+AGG | - | chr2.1:15643384-15643403 | MS.gene35410:CDS | 30.0% | |
| AATCAAGGTCACAAAGATTA+GGG | - | chr2.1:15643385-15643404 | MS.gene35410:CDS | 30.0% | |
| ATTTACGTACATCAGCAATA+AGG | + | chr2.1:15643368-15643387 | None:intergenic | 30.0% | |
| TTTACGTACATCAGCAATAA+GGG | + | chr2.1:15643367-15643386 | None:intergenic | 30.0% | |
| AGGAACAAAAGCTGAAAGAA+TGG | - | chr2.1:15643274-15643293 | MS.gene35410:CDS | 35.0% | |
| GGAACAAAAGCTGAAAGAAT+GGG | - | chr2.1:15643275-15643294 | MS.gene35410:CDS | 35.0% | |
| TTGCTGATGTACGTAAATCA+AGG | - | chr2.1:15643370-15643389 | MS.gene35410:CDS | 35.0% | |
| GCAGTAACAATCAATGCATG+AGG | + | chr2.1:15643336-15643355 | None:intergenic | 40.0% | |
| ! | AGGTTAAGGTACCTGAAAAC+TGG | - | chr2.1:15643247-15643266 | MS.gene35410:CDS | 40.0% |
| ! | GGTTAAGGTACCTGAAAACT+GGG | - | chr2.1:15643248-15643267 | MS.gene35410:CDS | 40.0% |
| ! | TGTTCCTTCTCCCAGTTTTC+AGG | + | chr2.1:15643261-15643280 | None:intergenic | 45.0% |
| GGTACCTGAAAACTGGGAGA+AGG | - | chr2.1:15643254-15643273 | MS.gene35410:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 15643237 | 15643419 | 15643237 | ID=MS.gene35410 |
| chr2.1 | mRNA | 15643237 | 15643419 | 15643237 | ID=MS.gene35410.t1;Parent=MS.gene35410 |
| chr2.1 | exon | 15643237 | 15643419 | 15643237 | ID=MS.gene35410.t1.exon1;Parent=MS.gene35410.t1 |
| chr2.1 | CDS | 15643237 | 15643419 | 15643237 | ID=cds.MS.gene35410.t1;Parent=MS.gene35410.t1 |
| Gene Sequence |
| Protein sequence |
