AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35456

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35456.t1 MTR_2g087270 94.560 386 21 0 1 386 1 386 0.0 763
MS.gene35456.t1 MTR_4g109500 30.102 392 232 13 8 385 10 373 8.49e-47 169
MS.gene35456.t1 MTR_4g109500 31.534 352 213 11 48 385 36 373 1.02e-46 169
MS.gene35456.t1 MTR_8g093800 32.063 315 191 7 48 344 75 384 1.83e-42 154
MS.gene35456.t1 MTR_4g111985 32.249 338 219 6 50 384 218 548 7.42e-42 155
MS.gene35456.t1 MTR_4g111985 35.357 280 173 4 50 327 218 491 1.01e-41 153
MS.gene35456.t1 MTR_4g111985 35.461 282 174 4 50 329 257 532 1.26e-41 155
MS.gene35456.t1 MTR_4g055080 28.667 300 182 9 85 368 34 317 1.13e-25 107
MS.gene35456.t1 MTR_3g118535 36.943 157 94 2 154 310 149 300 1.53e-25 108
MS.gene35456.t1 MTR_4g055080 28.667 300 182 9 85 368 59 342 1.53e-25 107
MS.gene35456.t1 MTR_3g077160 34.066 182 110 3 154 335 161 332 4.66e-25 106
MS.gene35456.t1 MTR_5g004640 36.306 157 95 2 154 310 152 303 5.42e-24 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35456.t1 AT1G08460 71.582 373 104 2 14 384 3 375 0.0 570
MS.gene35456.t1 AT1G08460 75.547 274 65 2 15 286 4 277 3.17e-156 442
MS.gene35456.t1 AT5G61060 30.051 396 235 11 4 381 14 385 1.72e-45 166
MS.gene35456.t1 AT5G61070 30.670 388 223 12 12 381 55 414 8.50e-45 164
MS.gene35456.t1 AT5G61060 30.051 396 231 12 4 381 14 381 1.46e-44 163
MS.gene35456.t1 AT4G33470 29.630 351 211 9 48 374 78 416 2.77e-42 153
MS.gene35456.t1 AT3G18520 35.106 282 175 4 48 327 169 444 5.63e-38 142
MS.gene35456.t1 AT3G18520 34.859 284 177 4 48 329 169 446 7.44e-38 143
MS.gene35456.t1 AT3G18520 34.859 284 177 4 48 329 169 446 7.98e-38 143
MS.gene35456.t1 AT3G18520 34.859 284 177 4 48 329 169 446 1.19e-37 143
MS.gene35456.t1 AT3G44680 28.667 300 182 9 85 368 59 342 2.01e-27 112
MS.gene35456.t1 AT5G63110 29.260 311 174 11 48 335 36 323 2.04e-26 110
MS.gene35456.t1 AT4G38130 35.669 157 96 2 154 310 148 299 8.48e-24 102
MS.gene35456.t1 AT4G38130 35.669 157 96 2 154 310 148 299 1.36e-23 102
MS.gene35456.t1 AT5G35600 33.333 186 102 5 154 335 147 314 2.64e-19 89.4

Find 104 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATATCAGGCTCAAATTTA+TGG 0.115466 2.1:+14981863 None:intergenic
CTATCTCTCCTTATGTTTCT+TGG 0.167482 2.1:-14983533 MS.gene35456:CDS
ATGGAAGGCTATAGAGAAAT+TGG 0.218703 2.1:-14981257 MS.gene35456:CDS
CGTCTCGTCCTCCGGATAAT+AGG 0.219824 2.1:+14981079 None:intergenic
GTACAGTTAGCTTTAGATTC+TGG 0.256578 2.1:-14982147 MS.gene35456:CDS
ATAGATATTGATGTTCATTA+TGG 0.271623 2.1:-14982105 MS.gene35456:CDS
ATTGATGTTCATTATGGAAA+TGG 0.351697 2.1:-14982099 MS.gene35456:CDS
TATGCTTTGGTTAGGCCTCC+TGG 0.361621 2.1:-14982231 MS.gene35456:CDS
GGCTCAAATTTATGGATTGA+TGG 0.362518 2.1:+14981871 None:intergenic
TTCCAACACGTCTAAGAAAC+CGG 0.362595 2.1:+14983614 None:intergenic
GATATGATTGTCTTGGTTGT+AGG 0.362797 2.1:-14981847 MS.gene35456:CDS
CTGCTAGACCGGCGTTGTTA+AGG 0.377714 2.1:+14982169 None:intergenic
ATACCTTTGCCAAATGGAAC+TGG 0.380604 2.1:-14981934 MS.gene35456:CDS
TGGAAAAGTTGCTTATGCTT+TGG 0.394414 2.1:-14982244 MS.gene35456:CDS
CCTTATGTTTCTTGGCACCT+TGG 0.399647 2.1:-14983525 MS.gene35456:CDS
TTCTGCTACCGCATCTGTAT+TGG 0.406722 2.1:+14983728 None:intergenic
CCATTGTCCCCAGTTCCATT+TGG 0.421588 2.1:+14981925 None:intergenic
TGTTTACATGCAACACTTGA+AGG 0.421754 2.1:-14981143 MS.gene35456:CDS
CTAGGTGATGGAAAAGGTTA+TGG 0.423056 2.1:-14981970 MS.gene35456:CDS
GTGTGAAAAGAAAGGAGCTC+AGG 0.423266 2.1:+14983486 None:intergenic
GTTTCTCTTCATATGAATCA+TGG 0.423891 2.1:-14982036 MS.gene35456:CDS
ACAGAAAACGTCTCGTCCTC+CGG 0.432609 2.1:+14981071 None:intergenic
TCACCATGCTCAGCCTTCTC+AGG 0.438971 2.1:-14982208 MS.gene35456:CDS
TTGAACATACCTTTGCCAAA+TGG 0.438998 2.1:-14981940 MS.gene35456:CDS
TTCATATGAATCATGGATCA+TGG 0.439204 2.1:-14982029 MS.gene35456:CDS
TGTTAAGCATTGCCTTCCAT+TGG 0.445060 2.1:+14981280 None:intergenic
ACAACCAAGACAATCATATC+AGG 0.457583 2.1:+14981850 None:intergenic
TCGATGAAGCACTTGCTTGA+CGG 0.460456 2.1:-14982270 MS.gene35456:CDS
GTTAAGCATTGCCTTCCATT+GGG 0.466444 2.1:+14981281 None:intergenic
CTCAGAGTACATAAATGAAC+TGG 0.467324 2.1:-14982394 MS.gene35456:intron
CATTCTTGAACCCTGGATCA+TGG 0.469920 2.1:-14982329 MS.gene35456:CDS
AGATTCTGTTGCCTATTATC+CGG 0.472439 2.1:-14981090 MS.gene35456:CDS
GCTCAGCCTTCTCAGGCTGA+TGG 0.478600 2.1:-14982201 MS.gene35456:CDS
AGCTAACTGTACTGCTAGAC+CGG 0.480094 2.1:+14982158 None:intergenic
AGTGCAGCATCCCATGATCC+AGG 0.481293 2.1:+14982318 None:intergenic
CTCAGCCTTCTCAGGCTGAT+GGG 0.482724 2.1:-14982200 MS.gene35456:CDS
ATGCTGCACTTCTTGCCGCC+GGG 0.484367 2.1:-14982305 MS.gene35456:CDS
TAATAGATAAAATGCCTTTG+TGG 0.486516 2.1:-14981019 MS.gene35456:CDS
CAAGAAACATAAGGAGAGAT+AGG 0.488178 2.1:+14983534 None:intergenic
TGTGGCACATTCTTGAACCC+TGG 0.495198 2.1:-14982336 MS.gene35456:CDS
AAGTTGCTTATGCTTTGGTT+AGG 0.495823 2.1:-14982239 MS.gene35456:CDS
CCCCGGTTTCTTAGACGTGT+TGG 0.500802 2.1:-14983616 MS.gene35456:CDS
GAAGGCTGAGCATGGTGACC+AGG 0.501428 2.1:+14982213 None:intergenic
TGGAAGGCTATAGAGAAATT+GGG 0.506833 2.1:-14981256 MS.gene35456:CDS
AAGCACTTGCTTGACGGACA+TGG 0.517006 2.1:-14982264 MS.gene35456:CDS
TGGACGACTTCTTATTGTGC+AGG 0.517764 2.1:-14981198 MS.gene35456:CDS
ATTCTTGAACCCTGGATCAT+GGG 0.518742 2.1:-14982328 MS.gene35456:CDS
GATGCTGCACTTCTTGCCGC+CGG 0.519018 2.1:-14982306 MS.gene35456:CDS
CTGGTAAAAGCTGATAAAGA+AGG 0.519535 2.1:-14982375 MS.gene35456:CDS
TCATATGAATCATGGATCAT+GGG 0.522086 2.1:-14982028 MS.gene35456:CDS
GTGTTTCAGTTTGATCCCAA+TGG 0.522130 2.1:-14981296 MS.gene35456:intron
TACCTTTGCCAAATGGAACT+GGG 0.522843 2.1:-14981933 MS.gene35456:CDS
CTTCTTATTGTGCAGGAAGG+TGG 0.526523 2.1:-14981191 MS.gene35456:CDS
ATGATTCATATGAAGAGAAA+CGG 0.532665 2.1:+14982038 None:intergenic
CCAAGGTGCCAAGAAACATA+AGG 0.533396 2.1:+14983525 None:intergenic
TCCAACACGTCTAAGAAACC+GGG 0.536668 2.1:+14983615 None:intergenic
GGTAAAAGCTGATAAAGAAG+GGG 0.539956 2.1:-14982373 MS.gene35456:CDS
TGAGCCTGATATGATTGTCT+TGG 0.544113 2.1:-14981854 MS.gene35456:CDS
TGTATATGCCTTCAATGAGT+TGG 0.547153 2.1:-14981899 MS.gene35456:CDS
TGGAACAACCAACTCATTGA+AGG 0.547472 2.1:+14981891 None:intergenic
GGAATGCTCAAACACGATAC+CGG 0.549251 2.1:-14983666 MS.gene35456:CDS
TCCCCAGTTCCATTTGGCAA+AGG 0.550741 2.1:+14981931 None:intergenic
GAACCACTCTGCGGATGAGA+TGG 0.551133 2.1:+14982003 None:intergenic
CCAAATGGAACTGGGGACAA+TGG 0.552008 2.1:-14981925 MS.gene35456:CDS
GATGAGCTAGGTGATGGAAA+AGG 0.557420 2.1:-14981976 MS.gene35456:CDS
ATGGTCTCAATCCTTAAAAG+AGG 0.557846 2.1:-14983558 MS.gene35456:CDS
AGGCAATGCTTAACAATGGA+AGG 0.558257 2.1:-14981272 MS.gene35456:CDS
TGGTAAAAGCTGATAAAGAA+GGG 0.562108 2.1:-14982374 MS.gene35456:CDS
CATCGAAGACAGTGTAGTCC+CGG 0.564565 2.1:+14982287 None:intergenic
TATGGAAATGGAACAGCGGA+GGG 0.571295 2.1:-14982087 MS.gene35456:CDS
TTCAGTTTGATCCCAATGGA+AGG 0.573033 2.1:-14981292 MS.gene35456:intron
CTCAAACACGATACCGGCAA+TGG 0.574453 2.1:-14983660 MS.gene35456:CDS
AGTGGTTCTATTGATGAGCT+AGG 0.577656 2.1:-14981988 MS.gene35456:CDS
AACCAGGAGTGTGAAAAGAA+AGG 0.581611 2.1:+14983478 None:intergenic
GCAACAGAATCTTGTAGTAG+AGG 0.582073 2.1:+14981101 None:intergenic
GAATCTTGTAGTAGAGGCAA+TGG 0.582393 2.1:+14981107 None:intergenic
TCATATGAAGAGAAACGGTA+AGG 0.587254 2.1:+14982043 None:intergenic
TCAAGAATGTGCCACAACAA+AGG 0.592402 2.1:+14982343 None:intergenic
CAGTACCCATCAGCCTGAGA+AGG 0.593929 2.1:+14982195 None:intergenic
CGACTTCTTATTGTGCAGGA+AGG 0.594885 2.1:-14981194 MS.gene35456:CDS
TACTGTTTCCTTAACAACGC+CGG 0.598032 2.1:-14982177 MS.gene35456:CDS
CAGCCTGAGAAGGCTGAGCA+TGG 0.607738 2.1:+14982205 None:intergenic
TGGAAGGCAATGCTTAACAA+TGG 0.612899 2.1:-14981276 MS.gene35456:CDS
CATATGAATCATGGATCATG+GGG 0.613927 2.1:-14982027 MS.gene35456:CDS
TCTATTGATGAGCTAGGTGA+TGG 0.615901 2.1:-14981982 MS.gene35456:CDS
TGTGAAAAGAAAGGAGCTCA+GGG 0.616383 2.1:+14983487 None:intergenic
TTCTGTTGCCTATTATCCGG+AGG 0.618398 2.1:-14981087 MS.gene35456:CDS
GGCTGAGCATGGTGACCAGG+AGG 0.619749 2.1:+14982216 None:intergenic
AGTTAATCTGTTCTCCACAA+AGG 0.625888 2.1:+14981005 None:intergenic
ACCTTTGCCAAATGGAACTG+GGG 0.627049 2.1:-14981932 MS.gene35456:CDS
GGATGAGAGCATGTCGACCA+AGG 0.628418 2.1:+14983508 None:intergenic
GTGCAGCATCCCATGATCCA+GGG 0.634352 2.1:+14982319 None:intergenic
TTATGGAAATGGAACAGCGG+AGG 0.635964 2.1:-14982088 MS.gene35456:CDS
CTTGCGACGAGGCACAGTGA+TGG 0.646082 2.1:-14981218 MS.gene35456:CDS
TTTGACACAGGAATTGACCC+CGG 0.658755 2.1:-14983633 MS.gene35456:CDS
GTAAAAGCTGATAAAGAAGG+GGG 0.672379 2.1:-14982372 MS.gene35456:CDS
GGGCCATCTCATCCGCAGAG+TGG 0.701407 2.1:-14982006 MS.gene35456:CDS
TATGGCAACCAATACAGATG+CGG 0.714104 2.1:-14983736 MS.gene35456:CDS
CCAACACGTCTAAGAAACCG+GGG 0.721257 2.1:+14983616 None:intergenic
TAGTTCATAGTCTTGCGACG+AGG 0.735316 2.1:-14981229 MS.gene35456:CDS
CGAAGACAGTGTAGTCCCGG+CGG 0.735641 2.1:+14982290 None:intergenic
TCATTATGGAAATGGAACAG+CGG 0.751642 2.1:-14982091 MS.gene35456:CDS
TCATCAATAGAACCACTCTG+CGG 0.759817 2.1:+14981994 None:intergenic
ATATGAATCATGGATCATGG+GGG 0.775931 2.1:-14982026 MS.gene35456:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTATTACATAATTTTTAA+TGG + chr2.1:14983711-14983730 None:intergenic 10.0%
!! AAATACTGAAATGTTACAAA+TGG + chr2.1:14982180-14982199 None:intergenic 20.0%
!! AATACTGAAATGTTACAAAT+GGG + chr2.1:14982179-14982198 None:intergenic 20.0%
!! AGTTAGTAGTTATTTAACAT+TGG - chr2.1:14981361-14981380 MS.gene35456:intron 20.0%
!! ATAGATATTGATGTTCATTA+TGG - chr2.1:14982637-14982656 MS.gene35456:intron 20.0%
!! ATCTAATAAATAAACTAACC+AGG + chr2.1:14981283-14981302 None:intergenic 20.0%
!! TCTAAAAAGATACACTTTAA+AGG + chr2.1:14981570-14981589 None:intergenic 20.0%
!!! AACATTGGAAGTTAAATTTT+GGG - chr2.1:14981376-14981395 MS.gene35456:intron 20.0%
!!! GAACTTTTTAGAATTATCAT+AGG + chr2.1:14982096-14982115 None:intergenic 20.0%
!!! TAACATTGGAAGTTAAATTT+TGG - chr2.1:14981375-14981394 MS.gene35456:intron 20.0%
! ACCAACTTATAATGAGTTTA+TGG + chr2.1:14981989-14982008 None:intergenic 25.0%
! ACTATTAGAGAAAAGATAAG+AGG + chr2.1:14982248-14982267 None:intergenic 25.0%
! ATGATTCATATGAAGAGAAA+CGG + chr2.1:14982707-14982726 None:intergenic 25.0%
! ATTGATGTTCATTATGGAAA+TGG - chr2.1:14982643-14982662 MS.gene35456:intron 25.0%
! CAATGATTTGAATTCAAGAT+AGG + chr2.1:14981478-14981497 None:intergenic 25.0%
! CCAACTTATAATGAGTTTAT+GGG + chr2.1:14981988-14982007 None:intergenic 25.0%
! CTATTAGAGAAAAGATAAGA+GGG + chr2.1:14982247-14982266 None:intergenic 25.0%
! GAAAAGTAATTCAATGCATA+TGG + chr2.1:14981690-14981709 None:intergenic 25.0%
! GAAATAGCACAAAAAAGAAA+AGG + chr2.1:14982955-14982974 None:intergenic 25.0%
! TAATAGATAAAATGCCTTTG+TGG - chr2.1:14983723-14983742 MS.gene35456:CDS 25.0%
! TATGTAAAAACTTCACATGT+TGG + chr2.1:14982072-14982091 None:intergenic 25.0%
! TCACTTAAATTGTAAAAGCA+TGG + chr2.1:14981959-14981978 None:intergenic 25.0%
!! AAAAGATACACTTTAAAGGT+TGG + chr2.1:14981566-14981585 None:intergenic 25.0%
!! CATAATTCAGAATTCAAGTT+CGG - chr2.1:14982209-14982228 MS.gene35456:CDS 25.0%
!! TGTCAAATTGTTTGAAGTAT+AGG + chr2.1:14982296-14982315 None:intergenic 25.0%
!!! GAGTTTAATTTTAGTGAATG+TGG + chr2.1:14983421-14983440 None:intergenic 25.0%
AATAAGCTGAAAACAACTCA+TGG + chr2.1:14983151-14983170 None:intergenic 30.0%
ACATAAACGCTTATCAGATA+AGG + chr2.1:14983283-14983302 None:intergenic 30.0%
ATTCAATGCATATGGTTATC+AGG + chr2.1:14981682-14981701 None:intergenic 30.0%
CATAAACGCTTATCAGATAA+GGG + chr2.1:14983282-14983301 None:intergenic 30.0%
CATCACAAATCACAATGAAT+CGG + chr2.1:14981613-14981632 None:intergenic 30.0%
CCCATAAACTCATTATAAGT+TGG - chr2.1:14981985-14982004 MS.gene35456:CDS 30.0%
GTTTCTCTTCATATGAATCA+TGG - chr2.1:14982706-14982725 MS.gene35456:intron 30.0%
TCATATCAGGCTCAAATTTA+TGG + chr2.1:14982882-14982901 None:intergenic 30.0%
TCATATGAATCATGGATCAT+GGG - chr2.1:14982714-14982733 MS.gene35456:intron 30.0%
TGGTAAAAGCTGATAAAGAA+GGG - chr2.1:14982368-14982387 MS.gene35456:CDS 30.0%
TGGTTTCATTGCATTTGTTT+AGG + chr2.1:14983367-14983386 None:intergenic 30.0%
TTCATATGAATCATGGATCA+TGG - chr2.1:14982713-14982732 MS.gene35456:intron 30.0%
TTCTTATAGCTAAGATCAGT+TGG - chr2.1:14981717-14981736 MS.gene35456:intron 30.0%
! ACAGGATAATCAAACACTTT+TGG + chr2.1:14983387-14983406 None:intergenic 30.0%
! AGACTTGCATATGACTTTTA+TGG - chr2.1:14983021-14983040 MS.gene35456:intron 30.0%
! GACTTGCATATGACTTTTAT+GGG - chr2.1:14983022-14983041 MS.gene35456:intron 30.0%
! TGAACAAAATTGACGTTTTC+TGG - chr2.1:14981047-14981066 MS.gene35456:CDS 30.0%
! TTGTTTAGGTTTGCATATCA+TGG + chr2.1:14983353-14983372 None:intergenic 30.0%
! TTTGTGAAGATTGCATTTTC+TGG - chr2.1:14981399-14981418 MS.gene35456:intron 30.0%
!! GGTTTTATAAGCCAATTTGT+GGG - chr2.1:14982006-14982025 MS.gene35456:CDS 30.0%
!!! TGGTTTTATAAGCCAATTTG+TGG - chr2.1:14982005-14982024 MS.gene35456:CDS 30.0%
AAGTTGCTTATGCTTTGGTT+AGG - chr2.1:14982503-14982522 MS.gene35456:intron 35.0%
ACAACCAAGACAATCATATC+AGG + chr2.1:14982895-14982914 None:intergenic 35.0%
AGATTCTGTTGCCTATTATC+CGG - chr2.1:14983652-14983671 MS.gene35456:CDS 35.0%
ATATGAATCATGGATCATGG+GGG - chr2.1:14982716-14982735 MS.gene35456:intron 35.0%
ATGGAAGGCTATAGAGAAAT+TGG - chr2.1:14983485-14983504 MS.gene35456:CDS 35.0%
ATGGTCTCAATCCTTAAAAG+AGG - chr2.1:14981184-14981203 MS.gene35456:CDS 35.0%
ATTGTTCTCATAAGCTATCG+TGG + chr2.1:14983186-14983205 None:intergenic 35.0%
CAAGAAACATAAGGAGAGAT+AGG + chr2.1:14981211-14981230 None:intergenic 35.0%
CATATGAATCATGGATCATG+GGG - chr2.1:14982715-14982734 MS.gene35456:intron 35.0%
CCATAAATTTATCACTTCCG+AGG - chr2.1:14981801-14981820 MS.gene35456:intron 35.0%
CCTCGGAAGTGATAAATTTA+TGG + chr2.1:14981804-14981823 None:intergenic 35.0%
CTATCTCTCCTTATGTTTCT+TGG - chr2.1:14981209-14981228 MS.gene35456:CDS 35.0%
CTCAGAGTACATAAATGAAC+TGG - chr2.1:14982348-14982367 MS.gene35456:CDS 35.0%
CTGGTAAAAGCTGATAAAGA+AGG - chr2.1:14982367-14982386 MS.gene35456:CDS 35.0%
GATATGATTGTCTTGGTTGT+AGG - chr2.1:14982895-14982914 MS.gene35456:intron 35.0%
GGTAAAAGCTGATAAAGAAG+GGG - chr2.1:14982369-14982388 MS.gene35456:CDS 35.0%
GTAAAAGCTGATAAAGAAGG+GGG - chr2.1:14982370-14982389 MS.gene35456:CDS 35.0%
TATCGTGCTGTAAACAACTT+GGG - chr2.1:14981756-14981775 MS.gene35456:intron 35.0%
TCATATGAAGAGAAACGGTA+AGG + chr2.1:14982702-14982721 None:intergenic 35.0%
TCATTATGGAAATGGAACAG+CGG - chr2.1:14982651-14982670 MS.gene35456:intron 35.0%
TGGAAAAGTTGCTTATGCTT+TGG - chr2.1:14982498-14982517 MS.gene35456:intron 35.0%
TGGAAGGCTATAGAGAAATT+GGG - chr2.1:14983486-14983505 MS.gene35456:CDS 35.0%
TGTATATGCCTTCAATGAGT+TGG - chr2.1:14982843-14982862 MS.gene35456:intron 35.0%
TGTTCATAACACCCACAAAT+TGG + chr2.1:14982020-14982039 None:intergenic 35.0%
TGTTTACATGCAACACTTGA+AGG - chr2.1:14983599-14983618 MS.gene35456:CDS 35.0%
TTATCGTGCTGTAAACAACT+TGG - chr2.1:14981755-14981774 MS.gene35456:intron 35.0%
TTGAACATACCTTTGCCAAA+TGG - chr2.1:14982802-14982821 MS.gene35456:intron 35.0%
TTGAACTTCTAATGACTGCA+CGG - chr2.1:14981848-14981867 MS.gene35456:CDS 35.0%
TTGAAGTATAGGCTATAGCA+GGG + chr2.1:14982285-14982304 None:intergenic 35.0%
! AACGTCAATTTTGTTCACAC+TGG + chr2.1:14981044-14981063 None:intergenic 35.0%
! GCACATAATTGAGCACTTTT+CGG - chr2.1:14981900-14981919 MS.gene35456:CDS 35.0%
! GGCTCAAATTTATGGATTGA+TGG + chr2.1:14982874-14982893 None:intergenic 35.0%
! TGCATATGACTTTTATGGGA+TGG - chr2.1:14983026-14983045 MS.gene35456:intron 35.0%
! TTTGAAGTATAGGCTATAGC+AGG + chr2.1:14982286-14982305 None:intergenic 35.0%
!! ACACTTTAAAGGTTGGTGAA+TGG + chr2.1:14981559-14981578 None:intergenic 35.0%
!! ATTGACGTTTTCTGGTATGA+AGG - chr2.1:14981055-14981074 MS.gene35456:CDS 35.0%
!! GTACAGTTAGCTTTAGATTC+TGG - chr2.1:14982595-14982614 MS.gene35456:intron 35.0%
!!! AATGTGGCTTTGTTTTACAC+AGG + chr2.1:14983405-14983424 None:intergenic 35.0%
AAGCTATCGTGGAGAGTTTA+TGG + chr2.1:14983175-14983194 None:intergenic 40.0%
AATTGCTCCAGCTGAATTCA+AGG + chr2.1:14982130-14982149 None:intergenic 40.0%
AGGCAATGCTTAACAATGGA+AGG - chr2.1:14983470-14983489 MS.gene35456:intron 40.0%
ATACCTTTGCCAAATGGAAC+TGG - chr2.1:14982808-14982827 MS.gene35456:intron 40.0%
ATTCTTGAACCCTGGATCAT+GGG - chr2.1:14982414-14982433 MS.gene35456:intron 40.0%
CAGTATTCCTTGAATTCAGC+TGG - chr2.1:14982120-14982139 MS.gene35456:CDS 40.0%
GAATCTTGTAGTAGAGGCAA+TGG + chr2.1:14983638-14983657 None:intergenic 40.0%
GCAACAGAATCTTGTAGTAG+AGG + chr2.1:14983644-14983663 None:intergenic 40.0%
GTGTTTCAGTTTGATCCCAA+TGG - chr2.1:14983446-14983465 MS.gene35456:intron 40.0%
TACCTTTGCCAAATGGAACT+GGG - chr2.1:14982809-14982828 MS.gene35456:intron 40.0%
TACTGTTTCCTTAACAACGC+CGG - chr2.1:14982565-14982584 MS.gene35456:intron 40.0%
TCAAGAATGTGCCACAACAA+AGG + chr2.1:14982402-14982421 None:intergenic 40.0%
TCATCAATAGAACCACTCTG+CGG + chr2.1:14982751-14982770 None:intergenic 40.0%
TCTATTGATGAGCTAGGTGA+TGG - chr2.1:14982760-14982779 MS.gene35456:intron 40.0%
TGAGCCTGATATGATTGTCT+TGG - chr2.1:14982888-14982907 MS.gene35456:intron 40.0%
TGGAACAACCAACTCATTGA+AGG + chr2.1:14982854-14982873 None:intergenic 40.0%
TGGAAGGCAATGCTTAACAA+TGG - chr2.1:14983466-14983485 MS.gene35456:intron 40.0%
TGTGAAAAGAAAGGAGCTCA+GGG + chr2.1:14981258-14981277 None:intergenic 40.0%
TGTGTCAAAAACTCCATTGC+CGG + chr2.1:14981098-14981117 None:intergenic 40.0%
TTCAGTTTGATCCCAATGGA+AGG - chr2.1:14983450-14983469 MS.gene35456:intron 40.0%
TTCCAACACGTCTAAGAAAC+CGG + chr2.1:14981131-14981150 None:intergenic 40.0%
! AACCAGGAGTGTGAAAAGAA+AGG + chr2.1:14981267-14981286 None:intergenic 40.0%
! AGTGGTTCTATTGATGAGCT+AGG - chr2.1:14982754-14982773 MS.gene35456:intron 40.0%
! GAGAGATAGGACCTCTTTTA+AGG + chr2.1:14981198-14981217 None:intergenic 40.0%
! GTTAAGCATTGCCTTCCATT+GGG + chr2.1:14983464-14983483 None:intergenic 40.0%
! TGGGTGTTATGAACATGTCA+TGG - chr2.1:14982025-14982044 MS.gene35456:CDS 40.0%
! TGTTAAGCATTGCCTTCCAT+TGG + chr2.1:14983465-14983484 None:intergenic 40.0%
!! CTAGGTGATGGAAAAGGTTA+TGG - chr2.1:14982772-14982791 MS.gene35456:intron 40.0%
!! GACTTTTATGGGATGGACTT+AGG - chr2.1:14983033-14983052 MS.gene35456:intron 40.0%
AACTTCTAATGACTGCACGG+TGG - chr2.1:14981851-14981870 MS.gene35456:CDS 45.0%
ACCTTTGCCAAATGGAACTG+GGG - chr2.1:14982810-14982829 MS.gene35456:intron 45.0%
ACTTCTAATGACTGCACGGT+GGG - chr2.1:14981852-14981871 MS.gene35456:CDS 45.0%
AGCTAACTGTACTGCTAGAC+CGG + chr2.1:14982587-14982606 None:intergenic 45.0%
CATTCTTGAACCCTGGATCA+TGG - chr2.1:14982413-14982432 MS.gene35456:intron 45.0%
CCTTATGTTTCTTGGCACCT+TGG - chr2.1:14981217-14981236 MS.gene35456:CDS 45.0%
CGACTTCTTATTGTGCAGGA+AGG - chr2.1:14983548-14983567 MS.gene35456:CDS 45.0%
CGCAGACAGAGTCAAAAACA+TGG - chr2.1:14981165-14981184 MS.gene35456:CDS 45.0%
CTTCTTATTGTGCAGGAAGG+TGG - chr2.1:14983551-14983570 MS.gene35456:CDS 45.0%
GGAATGCTCAAACACGATAC+CGG - chr2.1:14981076-14981095 MS.gene35456:CDS 45.0%
GGTGTGTATTACGCTCTGTT+TGG + chr2.1:14983332-14983351 None:intergenic 45.0%
GTGTGAAAAGAAAGGAGCTC+AGG + chr2.1:14981259-14981278 None:intergenic 45.0%
TATGGAAATGGAACAGCGGA+GGG - chr2.1:14982655-14982674 MS.gene35456:intron 45.0%
TCCAACACGTCTAAGAAACC+GGG + chr2.1:14981130-14981149 None:intergenic 45.0%
TGGACGACTTCTTATTGTGC+AGG - chr2.1:14983544-14983563 MS.gene35456:CDS 45.0%
TTATGGAAATGGAACAGCGG+AGG - chr2.1:14982654-14982673 MS.gene35456:intron 45.0%
TTCTGCTACCGCATCTGTAT+TGG + chr2.1:14981017-14981036 None:intergenic 45.0%
TTCTGTTGCCTATTATCCGG+AGG - chr2.1:14983655-14983674 MS.gene35456:CDS 45.0%
TTTGACACAGGAATTGACCC+CGG - chr2.1:14981109-14981128 MS.gene35456:CDS 45.0%
! CCAAGGTGCCAAGAAACATA+AGG + chr2.1:14981220-14981239 None:intergenic 45.0%
! CTCCTTTCTTTTCACACTCC+TGG - chr2.1:14981262-14981281 MS.gene35456:CDS 45.0%
! GGATGGACTTAGGACATGTT+TGG - chr2.1:14983043-14983062 MS.gene35456:intron 45.0%
! GGCAATGGAGTTTTTGACAC+AGG - chr2.1:14981097-14981116 MS.gene35456:CDS 45.0%
! TAGTTCATAGTCTTGCGACG+AGG - chr2.1:14983513-14983532 MS.gene35456:CDS 45.0%
! TCGATGAAGCACTTGCTTGA+CGG - chr2.1:14982472-14982491 MS.gene35456:intron 45.0%
! TTGACTCTGTCTGCGTTTTC+AGG + chr2.1:14981160-14981179 None:intergenic 45.0%
!! GATGAGCTAGGTGATGGAAA+AGG - chr2.1:14982766-14982785 MS.gene35456:intron 45.0%
ACAGAAAACGTCTCGTCCTC+CGG + chr2.1:14983674-14983693 None:intergenic 50.0%
ACTTCCGAGGCACAGTAGAA+GGG - chr2.1:14981814-14981833 MS.gene35456:intron 50.0%
CATCGAAGACAGTGTAGTCC+CGG + chr2.1:14982458-14982477 None:intergenic 50.0%
CCAAATGGAACTGGGGACAA+TGG - chr2.1:14982817-14982836 MS.gene35456:intron 50.0%
CCAACACGTCTAAGAAACCG+GGG + chr2.1:14981129-14981148 None:intergenic 50.0%
CCATTGTCCCCAGTTCCATT+TGG + chr2.1:14982820-14982839 None:intergenic 50.0%
CTCAAACACGATACCGGCAA+TGG - chr2.1:14981082-14981101 MS.gene35456:CDS 50.0%
CTTCTAATGACTGCACGGTG+GGG - chr2.1:14981853-14981872 MS.gene35456:CDS 50.0%
GGGGGAAAGATCCTTTGTTG+TGG - chr2.1:14982388-14982407 MS.gene35456:CDS 50.0%
TATGCTTTGGTTAGGCCTCC+TGG - chr2.1:14982511-14982530 MS.gene35456:intron 50.0%
TCCCCAGTTCCATTTGGCAA+AGG + chr2.1:14982814-14982833 None:intergenic 50.0%
TGTGGCACATTCTTGAACCC+TGG - chr2.1:14982406-14982425 MS.gene35456:intron 50.0%
TTCTAATGACTGCACGGTGG+GGG - chr2.1:14981854-14981873 MS.gene35456:CDS 50.0%
! AAGCACTTGCTTGACGGACA+TGG - chr2.1:14982478-14982497 MS.gene35456:intron 50.0%
AGTGCAGCATCCCATGATCC+AGG + chr2.1:14982427-14982446 None:intergenic 55.0%
CACTTCCGAGGCACAGTAGA+AGG - chr2.1:14981813-14981832 MS.gene35456:intron 55.0%
CAGTACCCATCAGCCTGAGA+AGG + chr2.1:14982550-14982569 None:intergenic 55.0%
CCCCGGTTTCTTAGACGTGT+TGG - chr2.1:14981126-14981145 MS.gene35456:CDS 55.0%
CGTCTCGTCCTCCGGATAAT+AGG + chr2.1:14983666-14983685 None:intergenic 55.0%
CTCAGCCTTCTCAGGCTGAT+GGG - chr2.1:14982542-14982561 MS.gene35456:intron 55.0%
CTGCTAGACCGGCGTTGTTA+AGG + chr2.1:14982576-14982595 None:intergenic 55.0%
GAACCACTCTGCGGATGAGA+TGG + chr2.1:14982742-14982761 None:intergenic 55.0%
GCATCCCTTCTACTGTGCCT+CGG + chr2.1:14981821-14981840 None:intergenic 55.0%
GTGCAGCATCCCATGATCCA+GGG + chr2.1:14982426-14982445 None:intergenic 55.0%
TCACCATGCTCAGCCTTCTC+AGG - chr2.1:14982534-14982553 MS.gene35456:intron 55.0%
! GGATGAGAGCATGTCGACCA+AGG + chr2.1:14981237-14981256 None:intergenic 55.0%
ATGCTGCACTTCTTGCCGCC+GGG - chr2.1:14982437-14982456 MS.gene35456:intron 60.0%
CAGCCTGAGAAGGCTGAGCA+TGG + chr2.1:14982540-14982559 None:intergenic 60.0%
CGAAGACAGTGTAGTCCCGG+CGG + chr2.1:14982455-14982474 None:intergenic 60.0%
CTTGCGACGAGGCACAGTGA+TGG - chr2.1:14983524-14983543 MS.gene35456:CDS 60.0%
GAAGGCTGAGCATGGTGACC+AGG + chr2.1:14982532-14982551 None:intergenic 60.0%
GATGCTGCACTTCTTGCCGC+CGG - chr2.1:14982436-14982455 MS.gene35456:intron 60.0%
GCTCAGCCTTCTCAGGCTGA+TGG - chr2.1:14982541-14982560 MS.gene35456:intron 60.0%
GCACGGTGGGGGTTACTCAC+TGG - chr2.1:14981865-14981884 MS.gene35456:CDS 65.0%
GGCTGAGCATGGTGACCAGG+AGG + chr2.1:14982529-14982548 None:intergenic 65.0%
GGGCCATCTCATCCGCAGAG+TGG - chr2.1:14982736-14982755 MS.gene35456:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 14981007 14983757 14981007 ID=MS.gene35456
chr2.1 mRNA 14981007 14983757 14981007 ID=MS.gene35456.t1;Parent=MS.gene35456
chr2.1 exon 14983481 14983757 14983481 ID=MS.gene35456.t1.exon1;Parent=MS.gene35456.t1
chr2.1 CDS 14983481 14983757 14983481 ID=cds.MS.gene35456.t1;Parent=MS.gene35456.t1
chr2.1 exon 14981831 14982411 14981831 ID=MS.gene35456.t1.exon2;Parent=MS.gene35456.t1
chr2.1 CDS 14981831 14982411 14981831 ID=cds.MS.gene35456.t1;Parent=MS.gene35456.t1
chr2.1 exon 14981007 14981309 14981007 ID=MS.gene35456.t1.exon3;Parent=MS.gene35456.t1
chr2.1 CDS 14981007 14981309 14981007 ID=cds.MS.gene35456.t1;Parent=MS.gene35456.t1
Gene Sequence

>MS.gene35456

ATGGCAACCAATACAGATGCGGTAGCAGAATCTTCCAGTGTGAACAAAATTGACGTTTTCTGGTATGAAGGAATGCTCAAACACGATACCGGCAATGGAGTTTTTGACACAGGAATTGACCCCGGTTTCTTAGACGTGTTGGAAAAACATCCTGAAAACGCAGACAGAGTCAAAAACATGGTCTCAATCCTTAAAAGAGGTCCTATCTCTCCTTATGTTTCTTGGCACCTTGGTCGACATGCTCTCATCCCTGAGCTCCTTTCTTTTCACACTCCTGGTTAGTTTATTTATTAGATAAAAATTTATGCTTTGCATGCAAAGCTGAAACTTTATAGATGTGAGTTCAGTCACTTTAGTTAGTAGTTATTTAACATTGGAAGTTAAATTTTGGGTTTGTGAAGATTGCATTTTCTGGAATAGATATTTTGTGCACACTGTATTGATGTACAGTATAATTCAGACATGAATCCTATCTTGAATTCAAATCATTGCGATAGTTTAATGTGCTGTGTGCAGCATATTATCTTATGATACATGCGAATTGCATCTCCATTCACCAACCTTTAAAGTGTATCTTTTTAGACATGAATCTCATCTTGAATTCCGATTCATTGTGATTTGTGATGAATCTTGATTCACAAAAAAGATCTTGCAACACTATTTCAATCTCGACCTGATAACCATATGCATTGAATTACTTTTCTAGATCATTCTTATAGCTAAGATCAGTTGGCAGTTCGTTAGTTTGTTATCGTGCTGTAAACAACTTGGGCAATGTTTAGATCTGCAGTGCACCATAAATTTATCACTTCCGAGGCACAGTAGAAGGGATGCTATACATTTGAACTTCTAATGACTGCACGGTGGGGGTTACTCACTGGTCTTACAAACATGCACATAATTGAGCACTTTTCGGTAAACATTTTTCATATAAGAGCTTATAGATACACCATGCTTTTACAATTTAAGTGAAAGTGTCCCATAAACTCATTATAAGTTGGTTTTATAAGCCAATTTGTGGGTGTTATGAACATGTCATGGACTATTTTTCATAAATTCTTTCCAACATGTGAAGTTTTTACATATCCTATGATAATTCTAAAAAGTTCTTTTCAGTATTCCTTGAATTCAGCTGGAGCAATTCTGCTACAAACACACTATATATATAACCCATTTGTAACATTTCAGTATTTTTGAGAAATCATAATTCAGAATTCAAGTTCGGTTTTCACAATTTCCCTCTTATCTTTTCTCTAATAGTCTCTCATTTTCTTACCCTGCTATAGCCTATACTTCAAACAATTTGACACAGTGAATGATATAATTTCAATGCTGTAATTTCTCAGAGTACATAAATGAACTGGTAAAAGCTGATAAAGAAGGGGGAAAGATCCTTTGTTGTGGCACATTCTTGAACCCTGGATCATGGGATGCTGCACTTCTTGCCGCCGGGACTACACTGTCTTCGATGAAGCACTTGCTTGACGGACATGGAAAAGTTGCTTATGCTTTGGTTAGGCCTCCTGGTCACCATGCTCAGCCTTCTCAGGCTGATGGGTACTGTTTCCTTAACAACGCCGGTCTAGCAGTACAGTTAGCTTTAGATTCTGGATGTAAGAAAGTTGCAGTTATAGATATTGATGTTCATTATGGAAATGGAACAGCGGAGGGATTTTATTCATCTGATAAAGTCCTTACCGTTTCTCTTCATATGAATCATGGATCATGGGGGCCATCTCATCCGCAGAGTGGTTCTATTGATGAGCTAGGTGATGGAAAAGGTTATGGTTATAACTTGAACATACCTTTGCCAAATGGAACTGGGGACAATGGATATGTATATGCCTTCAATGAGTTGGTTGTTCCATCAATCCATAAATTTGAGCCTGATATGATTGTCTTGGTTGTAGGACAAGACTCGAGTGCAGTAAGTTATTCTATTTATCCTTTTCTTTTTTGTGCTATTTCAATTTATAGTTTGATTGTGATTAGCTTTTTATTGACAAGTTTTTGTAGACTTGCATATGACTTTTATGGGATGGACTTAGGACATGTTTGGATTAACTTATTTGAGTTTATCTACTGACATAAGCACTTGTGAGACTATTTGAGAGAGCTTATGAAAACAGCTTATTGCATGTCCATGAGTTGTTTTCAGCTTATTTCCATAAACTCTCCACGATAGCTTATGAGAACAATTTATTACTTATATGACAACAATTCGACTTTATTTTATCTTTTGTTATAGAAATAGAAACAACTTATACATAAGCCCTTATCTGATAAGCGTTTATGTTATAAACGCTTAACTATATTGTTTATCCAAACAGAGCGTAATACACACCATGATATGCAAACCTAAACAAATGCAATGAAACCAAAAGTGTTTGATTATCCTGTGTAAAACAAAGCCACATTCACTAAAATTAAACTCTTCATGTGTTTCAGTTTGATCCCAATGGAAGGCAATGCTTAACAATGGAAGGCTATAGAGAAATTGGGCGTATAGTTCATAGTCTTGCGACGAGGCACAGTGATGGACGACTTCTTATTGTGCAGGAAGGTGGATATCATGTAACATATTCAGCATATTGTTTACATGCAACACTTGAAGGTGTTCTCAATCTACCATTGCCTCTACTACAAGATTCTGTTGCCTATTATCCGGAGGACGAGACGTTTTCTGTCAAAGTTATAGACGCCATTAAAAATTATGTAATAGATAAAATGCCTTTGTGGAGAACAGATTAA
Protein sequence

>MS.gene35456.t1

MATNTDAVAESSSVNKIDVFWYEGMLKHDTGNGVFDTGIDPGFLDVLEKHPENADRVKNMVSILKRGPISPYVSWHLGRHALIPELLSFHTPEYINELVKADKEGGKILCCGTFLNPGSWDAALLAAGTTLSSMKHLLDGHGKVAYALVRPPGHHAQPSQADGYCFLNNAGLAVQLALDSGCKKVAVIDIDVHYGNGTAEGFYSSDKVLTVSLHMNHGSWGPSHPQSGSIDELGDGKGYGYNLNIPLPNGTGDNGYVYAFNELVVPSIHKFEPDMIVLVVGQDSSAFDPNGRQCLTMEGYREIGRIVHSLATRHSDGRLLIVQEGGYHVTYSAYCLHATLEGVLNLPLPLLQDSVAYYPEDETFSVKVIDAIKNYVIDKMPLWRTD