Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35764.t1 | XP_003629262.1 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 2.00E-58 | 235 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35764.t1 | Q8L7E9 | 80.8 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 1.8e-52 | 206.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35764.t1 | G7LB65 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 1.4e-58 | 235.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049786 | MS.gene35764 | 0.819663 | 1.03E-52 | -1.69E-46 |
| MS.gene053677 | MS.gene35764 | 0.800345 | 1.51E-48 | -1.69E-46 |
| MS.gene055603 | MS.gene35764 | 0.814925 | 1.20E-51 | -1.69E-46 |
| MS.gene056638 | MS.gene35764 | 0.839043 | 2.01E-57 | -1.69E-46 |
| MS.gene057360 | MS.gene35764 | 0.829596 | 4.71E-55 | -1.69E-46 |
| MS.gene06011 | MS.gene35764 | 0.81179 | 5.87E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35764.t1 | MTR_8g075150 | 93.600 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 3.33e-81 | 244 |
| MS.gene35764.t1 | MTR_5g022670 | 82.114 | 123 | 22 | 0 | 3 | 125 | 2 | 124 | 3.84e-71 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35764.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.12e-70 | 215 |
| MS.gene35764.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.12e-70 | 215 |
| MS.gene35764.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.12e-70 | 215 |
| MS.gene35764.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.12e-70 | 215 |
| MS.gene35764.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.12e-70 | 215 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-58 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 6.02e-58 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 9.91e-58 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 9.91e-58 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.11e-57 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.11e-57 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.11e-57 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.11e-57 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.11e-57 | 184 |
| MS.gene35764.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-57 | 184 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGAGGCTCTCGGGTTTC+AGG | 0.140533 | 2.1:-12817351 | None:intergenic |
| CTGCATCTAAGCCTATTAAT+TGG | 0.182088 | 2.1:-12817191 | None:intergenic |
| TTTGAGAAGGTCTTCTATTT+TGG | 0.212238 | 2.1:+12817389 | MS.gene35764:CDS |
| GGAACGTTGTTTATCAATTT+AGG | 0.249469 | 2.1:+12817460 | MS.gene35764:CDS |
| GCAAATGTAGCCCAATTAAT+AGG | 0.272486 | 2.1:+12817180 | MS.gene35764:CDS |
| GCATCTTCATGGGAACAATT+TGG | 0.278027 | 2.1:+12817150 | MS.gene35764:CDS |
| GCACAACATCTTAAGTTGAT+TGG | 0.319124 | 2.1:+12817285 | MS.gene35764:CDS |
| TAGATGCAGTAAAGTTGATT+AGG | 0.362979 | 2.1:+12817205 | MS.gene35764:CDS |
| CACAACATCTTAAGTTGATT+GGG | 0.378407 | 2.1:+12817286 | MS.gene35764:CDS |
| CTAGTTGTTCTAGAGGCTCT+CGG | 0.379192 | 2.1:-12817359 | None:intergenic |
| GATATAGAATGGCATCTTCA+TGG | 0.416346 | 2.1:+12817139 | None:intergenic |
| TGCATCTAAGCCTATTAATT+GGG | 0.419039 | 2.1:-12817190 | None:intergenic |
| CTAGAGGCTCTCGGGTTTCA+GGG | 0.434211 | 2.1:-12817350 | None:intergenic |
| CTATCTTTATTTGCTCGCCA+TGG | 0.439513 | 2.1:+12817434 | MS.gene35764:CDS |
| TATCTTTATTTGCTCGCCAT+GGG | 0.488601 | 2.1:+12817435 | MS.gene35764:CDS |
| TAGTTGTTCTAGAGGCTCTC+GGG | 0.496041 | 2.1:-12817358 | None:intergenic |
| TTTATTTGCTCGCCATGGGA+TGG | 0.519477 | 2.1:+12817439 | MS.gene35764:CDS |
| ATATAGAATGGCATCTTCAT+GGG | 0.542232 | 2.1:+12817140 | None:intergenic |
| GCATCTTCTAGTTGTTCTAG+AGG | 0.556880 | 2.1:-12817366 | None:intergenic |
| CGTTGTTTATCAATTTAGGA+AGG | 0.558364 | 2.1:+12817464 | MS.gene35764:CDS |
| ATAAACAACGTTCCATCCCA+TGG | 0.601527 | 2.1:-12817451 | None:intergenic |
| AACTAGAAGATGCTTTGAGA+AGG | 0.613004 | 2.1:+12817376 | MS.gene35764:CDS |
| CGAAAGCAGCAAATACAGCA+AGG | 0.638455 | 2.1:+12817235 | MS.gene35764:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTTGTTTATCAATTTAGGA+AGG | + | chr2.1:12817464-12817483 | MS.gene35764:CDS | 30.0% | |
| GGAACGTTGTTTATCAATTT+AGG | + | chr2.1:12817460-12817479 | MS.gene35764:CDS | 30.0% | |
| TGCATCTAAGCCTATTAATT+GGG | - | chr2.1:12817193-12817212 | None:intergenic | 30.0% | |
| ! | TAGATGCAGTAAAGTTGATT+AGG | + | chr2.1:12817205-12817224 | MS.gene35764:CDS | 30.0% |
| ! | TTTGAGAAGGTCTTCTATTT+TGG | + | chr2.1:12817389-12817408 | MS.gene35764:CDS | 30.0% |
| !! | CACAACATCTTAAGTTGATT+GGG | + | chr2.1:12817286-12817305 | MS.gene35764:CDS | 30.0% |
| !!! | TATTTTGGTCAATAGTTGTC+AGG | + | chr2.1:12817404-12817423 | MS.gene35764:CDS | 30.0% |
| CTGCATCTAAGCCTATTAAT+TGG | - | chr2.1:12817194-12817213 | None:intergenic | 35.0% | |
| GCAAATGTAGCCCAATTAAT+AGG | + | chr2.1:12817180-12817199 | MS.gene35764:CDS | 35.0% | |
| ! | TATCTTTATTTGCTCGCCAT+GGG | + | chr2.1:12817435-12817454 | MS.gene35764:CDS | 35.0% |
| !! | AACTAGAAGATGCTTTGAGA+AGG | + | chr2.1:12817376-12817395 | MS.gene35764:CDS | 35.0% |
| !! | GCACAACATCTTAAGTTGAT+TGG | + | chr2.1:12817285-12817304 | MS.gene35764:CDS | 35.0% |
| ATAAACAACGTTCCATCCCA+TGG | - | chr2.1:12817454-12817473 | None:intergenic | 40.0% | |
| GCATCTTCATGGGAACAATT+TGG | + | chr2.1:12817150-12817169 | MS.gene35764:CDS | 40.0% | |
| ! | CTATCTTTATTTGCTCGCCA+TGG | + | chr2.1:12817434-12817453 | MS.gene35764:CDS | 40.0% |
| ! | GCATCTTCTAGTTGTTCTAG+AGG | - | chr2.1:12817369-12817388 | None:intergenic | 40.0% |
| CGAAAGCAGCAAATACAGCA+AGG | + | chr2.1:12817235-12817254 | MS.gene35764:CDS | 45.0% | |
| TTTATTTGCTCGCCATGGGA+TGG | + | chr2.1:12817439-12817458 | MS.gene35764:CDS | 45.0% | |
| !! | CTAGTTGTTCTAGAGGCTCT+CGG | - | chr2.1:12817362-12817381 | None:intergenic | 45.0% |
| !! | TAGTTGTTCTAGAGGCTCTC+GGG | - | chr2.1:12817361-12817380 | None:intergenic | 45.0% |
| CTAGAGGCTCTCGGGTTTCA+GGG | - | chr2.1:12817353-12817372 | None:intergenic | 55.0% | |
| TCTAGAGGCTCTCGGGTTTC+AGG | - | chr2.1:12817354-12817373 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 12817147 | 12817527 | 12817147 | ID=MS.gene35764 |
| chr2.1 | mRNA | 12817147 | 12817527 | 12817147 | ID=MS.gene35764.t1;Parent=MS.gene35764 |
| chr2.1 | exon | 12817147 | 12817527 | 12817147 | ID=MS.gene35764.t1.exon1;Parent=MS.gene35764.t1 |
| chr2.1 | CDS | 12817147 | 12817527 | 12817147 | ID=cds.MS.gene35764.t1;Parent=MS.gene35764.t1 |
| Gene Sequence |
| Protein sequence |
