Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36482.t1 | XP_003608488.2 | 96.6 | 350 | 12 | 0 | 1 | 350 | 1 | 350 | 2.10E-185 | 658.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36482.t1 | Q402F4 | 23.4 | 295 | 166 | 8 | 21 | 310 | 38 | 277 | 1.2e-08 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36482.t1 | G7JDY4 | 96.6 | 350 | 12 | 0 | 1 | 350 | 1 | 350 | 1.5e-185 | 658.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051245 | MS.gene36482 | 0.808303 | 3.31E-50 | -1.69E-46 |
| MS.gene053437 | MS.gene36482 | 0.800731 | 1.26E-48 | -1.69E-46 |
| MS.gene055174 | MS.gene36482 | 0.802001 | 6.90E-49 | -1.69E-46 |
| MS.gene057451 | MS.gene36482 | -0.815144 | 1.07E-51 | -1.69E-46 |
| MS.gene057452 | MS.gene36482 | -0.835684 | 1.45E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36482.t1 | MTR_4g096650 | 96.571 | 350 | 12 | 0 | 1 | 350 | 1 | 350 | 0.0 | 693 |
| MS.gene36482.t1 | MTR_4g096650 | 96.839 | 348 | 11 | 0 | 1 | 348 | 1 | 348 | 0.0 | 692 |
| MS.gene36482.t1 | MTR_4g096650 | 96.667 | 240 | 8 | 0 | 111 | 350 | 10 | 249 | 3.75e-172 | 479 |
| MS.gene36482.t1 | MTR_4g096650 | 97.059 | 238 | 7 | 0 | 111 | 348 | 10 | 247 | 9.47e-172 | 478 |
| MS.gene36482.t1 | MTR_5g091370 | 43.220 | 354 | 186 | 4 | 8 | 350 | 6 | 355 | 8.33e-85 | 261 |
| MS.gene36482.t1 | MTR_5g091370 | 43.172 | 227 | 117 | 2 | 8 | 226 | 6 | 228 | 1.72e-50 | 171 |
| MS.gene36482.t1 | MTR_4g094212 | 24.917 | 301 | 164 | 10 | 21 | 315 | 39 | 283 | 1.63e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36482.t1 | AT3G59090 | 43.875 | 351 | 189 | 3 | 9 | 352 | 15 | 364 | 2.07e-88 | 271 |
| MS.gene36482.t1 | AT3G59090 | 43.966 | 348 | 187 | 3 | 9 | 349 | 15 | 361 | 7.25e-88 | 270 |
| MS.gene36482.t1 | AT3G59090 | 44.308 | 325 | 173 | 3 | 35 | 352 | 35 | 358 | 1.54e-83 | 258 |
| MS.gene36482.t1 | AT3G59090 | 42.904 | 303 | 165 | 3 | 57 | 352 | 1 | 302 | 9.18e-72 | 226 |
| MS.gene36482.t1 | AT4G21790 | 22.109 | 294 | 171 | 8 | 25 | 314 | 45 | 284 | 4.49e-12 | 66.6 |
| MS.gene36482.t1 | AT1G14530 | 24.150 | 294 | 173 | 5 | 20 | 313 | 42 | 285 | 8.87e-11 | 62.8 |
Find 68 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCCATTGCTTCTATTC+AGG | 0.192960 | 4.1:-18399717 | MS.gene36482:intron |
| TGCAGGTTACTCCATTTATT+TGG | 0.193136 | 4.1:-18395625 | MS.gene36482:intron |
| TATTGATTGGCTCTTCTAAT+TGG | 0.200988 | 4.1:-18398267 | MS.gene36482:CDS |
| ATTGATTGGCTCTTCTAATT+GGG | 0.264343 | 4.1:-18398266 | MS.gene36482:CDS |
| TACTTGGATATTTCTGCTAT+TGG | 0.264487 | 4.1:-18392757 | MS.gene36482:CDS |
| TGCTCATCTTCAAGCATAAT+AGG | 0.275987 | 4.1:+18391765 | None:intergenic |
| GAGTATCAATGCCAAATAAA+TGG | 0.302200 | 4.1:+18395614 | None:intergenic |
| TGAGAAACCTTCATCTGAAA+TGG | 0.328551 | 4.1:-18391732 | MS.gene36482:CDS |
| TGGCATTTGCTGTAATAATC+TGG | 0.345239 | 4.1:-18393748 | MS.gene36482:CDS |
| TTTCTCTGAGGAGCCTTTGC+TGG | 0.351840 | 4.1:-18394037 | MS.gene36482:CDS |
| CAGGTGTTTCATCTATTGAT+TGG | 0.362217 | 4.1:-18398280 | MS.gene36482:CDS |
| AGATCTCATGCAGGTGTACT+TGG | 0.371929 | 4.1:-18392773 | MS.gene36482:intron |
| AGCTACAAGCCTACAGAATC+AGG | 0.393975 | 4.1:-18390849 | MS.gene36482:intron |
| CCTTCAGCCATGGCGGGATT+CGG | 0.395466 | 4.1:-18399809 | None:intergenic |
| ACCTTTCCCATTTCAGATGA+AGG | 0.401135 | 4.1:+18391725 | None:intergenic |
| TTTGCTGTAATAATCTGGAT+TGG | 0.421058 | 4.1:-18393743 | MS.gene36482:CDS |
| CACTTGAGAAGTTCAGAGCT+TGG | 0.421460 | 4.1:-18399072 | MS.gene36482:CDS |
| CAGCCATGGCGGGATTCGGT+GGG | 0.427029 | 4.1:-18399805 | None:intergenic |
| GGCGCAGCACTGGCATGTTA+TGG | 0.442563 | 4.1:-18392721 | MS.gene36482:intron |
| TTGCTGTAATAATCTGGATT+GGG | 0.444019 | 4.1:-18393742 | MS.gene36482:CDS |
| CTTGCAAGGGATGGACATAT+TGG | 0.447221 | 4.1:-18395584 | MS.gene36482:CDS |
| TATTGCAGATGATGTTTCAC+TGG | 0.449421 | 4.1:-18391290 | MS.gene36482:intron |
| AGCTGCCACTTCAGAGTCAA+TGG | 0.450555 | 4.1:+18393706 | None:intergenic |
| TCGTCAAAAGATTGTGACTT+TGG | 0.452661 | 4.1:-18393932 | MS.gene36482:intron |
| GATACTCCTTGCTGCTTGCA+AGG | 0.453979 | 4.1:-18395598 | MS.gene36482:CDS |
| GAGAAACCTTCATCTGAAAT+GGG | 0.463494 | 4.1:-18391731 | MS.gene36482:CDS |
| TCAGCCATGGCGGGATTCGG+TGG | 0.465741 | 4.1:-18399806 | None:intergenic |
| TGACTCTGAAGTGGCAGCTC+GGG | 0.467323 | 4.1:-18393702 | MS.gene36482:intron |
| TTGATTGGCTCTTCTAATTG+GGG | 0.473554 | 4.1:-18398265 | MS.gene36482:intron |
| TTCTCTGAGGAGCCTTTGCT+GGG | 0.473938 | 4.1:-18394036 | MS.gene36482:CDS |
| GCCATGGCGGGATTCGGTGG+GGG | 0.481359 | 4.1:-18399803 | MS.gene36482:CDS |
| GTAATAATCTGGATTGGGCT+AGG | 0.488481 | 4.1:-18393737 | MS.gene36482:CDS |
| TCATAGTCATCGTCATCGTC+TGG | 0.494305 | 4.1:+18394066 | None:intergenic |
| CTGCTCATCATCCTCAGCGC+TGG | 0.496733 | 4.1:-18390877 | MS.gene36482:CDS |
| TGAGAAGTTCAGAGCTTGGT+TGG | 0.498394 | 4.1:-18399068 | MS.gene36482:CDS |
| CAACAACCTTCAGCCATGGC+GGG | 0.503548 | 4.1:-18399815 | None:intergenic |
| GTCCGTTAAAAGTTCTATGC+AGG | 0.507440 | 4.1:+18391406 | None:intergenic |
| TCCTTGCTGCTTGCAAGGGA+TGG | 0.511552 | 4.1:-18395593 | MS.gene36482:CDS |
| ATAACAGCGAAGAGTGGATA+CGG | 0.515410 | 4.1:+18399770 | None:intergenic |
| ACTACATCAGCAGCTTCTGC+TGG | 0.521458 | 4.1:+18390944 | None:intergenic |
| CATCGTCTGGCTGATGGCAT+AGG | 0.522540 | 4.1:+18394079 | None:intergenic |
| CATCGTCATCGTCTGGCTGA+TGG | 0.524421 | 4.1:+18394073 | None:intergenic |
| TTTGAAGTCTTTCCCAGCAA+AGG | 0.527404 | 4.1:+18394024 | None:intergenic |
| AGCCATGGCGGGATTCGGTG+GGG | 0.535819 | 4.1:-18399804 | None:intergenic |
| TTTGGGTACCCCAGTTGCTG+AGG | 0.538939 | 4.1:-18399098 | MS.gene36482:intron |
| AATAACCTGAATAGAAGCAA+TGG | 0.542380 | 4.1:+18399712 | None:intergenic |
| ATACTCCTTGCTGCTTGCAA+GGG | 0.548818 | 4.1:-18395597 | MS.gene36482:CDS |
| CTATAGGGACCTGATTCTGT+AGG | 0.549428 | 4.1:+18390840 | None:intergenic |
| TTGACTCTGAAGTGGCAGCT+CGG | 0.554489 | 4.1:-18393703 | MS.gene36482:CDS |
| AGAAGCTGCTGATGTAGTAG+AGG | 0.555475 | 4.1:-18390939 | MS.gene36482:CDS |
| GCTGCCACTTCAGAGTCAAT+GGG | 0.557643 | 4.1:+18393707 | None:intergenic |
| GACGATGACGATGACTATGA+AGG | 0.575118 | 4.1:-18394063 | MS.gene36482:CDS |
| ACATATTGGTCACACTCTTG+TGG | 0.590797 | 4.1:-18395570 | MS.gene36482:CDS |
| GAATCCCATTGACTCTGAAG+TGG | 0.592710 | 4.1:-18393711 | MS.gene36482:CDS |
| ACCTTCAGCAGTAGTACTGT+GGG | 0.592883 | 4.1:-18390984 | MS.gene36482:CDS |
| ACCCACAGTACTACTGCTGA+AGG | 0.597180 | 4.1:+18390983 | None:intergenic |
| TACCTTCAGCAGTAGTACTG+TGG | 0.598958 | 4.1:-18390985 | MS.gene36482:CDS |
| ACCTTCATCTGAAATGGGAA+AGG | 0.606004 | 4.1:-18391726 | MS.gene36482:intron |
| ACAATGATAACAGCGAAGAG+TGG | 0.619986 | 4.1:+18399764 | None:intergenic |
| TCCATCCCTTGCAAGCAGCA+AGG | 0.625070 | 4.1:+18395592 | None:intergenic |
| ATACAAAATCAGCAGAAGAG+AGG | 0.630986 | 4.1:+18391239 | None:intergenic |
| TAACAGCGAAGAGTGGATAC+GGG | 0.634294 | 4.1:+18399771 | None:intergenic |
| ACATCAGCAGCTTCTGCTGG+CGG | 0.642156 | 4.1:+18390947 | None:intergenic |
| GAATCGCGTGTACAATAGCA+AGG | 0.645140 | 4.1:+18399741 | None:intergenic |
| TATTACAGCAAATGCCACAA+TGG | 0.656668 | 4.1:+18393754 | None:intergenic |
| AGAGTGGATACGGGAAACAG+TGG | 0.678735 | 4.1:+18399780 | None:intergenic |
| GTAGCTGTAGTCCAGCGCTG+AGG | 0.700160 | 4.1:+18390866 | None:intergenic |
| CTATGAAGGAAGTTTCTCTG+AGG | 0.700251 | 4.1:-18394049 | MS.gene36482:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 18390465 | 18399822 | 18390465 | ID=MS.gene36482 |
| chr4.1 | mRNA | 18390465 | 18399822 | 18390465 | ID=MS.gene36482.t1;Parent=MS.gene36482 |
| chr4.1 | exon | 18399718 | 18399822 | 18399718 | ID=MS.gene36482.t1.exon1;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18399718 | 18399822 | 18399718 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18399056 | 18399106 | 18399056 | ID=MS.gene36482.t1.exon2;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18399056 | 18399106 | 18399056 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18398266 | 18398299 | 18398266 | ID=MS.gene36482.t1.exon3;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18398266 | 18398299 | 18398266 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18395556 | 18395642 | 18395556 | ID=MS.gene36482.t1.exon4;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18395556 | 18395642 | 18395556 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18395416 | 18395467 | 18395416 | ID=MS.gene36482.t1.exon5;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18395416 | 18395467 | 18395416 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18393933 | 18394107 | 18393933 | ID=MS.gene36482.t1.exon6;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18393933 | 18394107 | 18393933 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18393703 | 18393798 | 18393703 | ID=MS.gene36482.t1.exon7;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18393703 | 18393798 | 18393703 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18392722 | 18392782 | 18392722 | ID=MS.gene36482.t1.exon8;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18392722 | 18392782 | 18392722 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18391727 | 18391791 | 18391727 | ID=MS.gene36482.t1.exon9;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18391727 | 18391791 | 18391727 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18391400 | 18391474 | 18391400 | ID=MS.gene36482.t1.exon10;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18391400 | 18391474 | 18391400 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18391229 | 18391304 | 18391229 | ID=MS.gene36482.t1.exon11;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18391229 | 18391304 | 18391229 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18390850 | 18391016 | 18390850 | ID=MS.gene36482.t1.exon12;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18390850 | 18391016 | 18390850 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| chr4.1 | exon | 18390465 | 18390527 | 18390465 | ID=MS.gene36482.t1.exon13;Parent=MS.gene36482.t1 |
| chr4.1 | CDS | 18390465 | 18390527 | 18390465 | ID=cds.MS.gene36482.t1;Parent=MS.gene36482.t1 |
| Gene Sequence |
| Protein sequence |
