Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36918.t1 | GAU44130.1 | 96 | 99 | 4 | 0 | 1 | 99 | 1 | 99 | 1.60E-47 | 198.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36918.t1 | A0MDQ1 | 81.7 | 93 | 17 | 0 | 7 | 99 | 9 | 101 | 8.9e-39 | 160.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36918.t1 | A0A2Z6P4S1 | 96.0 | 99 | 4 | 0 | 1 | 99 | 1 | 99 | 1.2e-47 | 198.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055162 | MS.gene36918 | 0.806784 | 6.95E-50 | -1.69E-46 |
| MS.gene057140 | MS.gene36918 | 0.824691 | 7.04E-54 | -1.69E-46 |
| MS.gene058182 | MS.gene36918 | 0.800401 | 1.47E-48 | -1.69E-46 |
| MS.gene058906 | MS.gene36918 | 0.831214 | 1.90E-55 | -1.69E-46 |
| MS.gene059898 | MS.gene36918 | 0.822019 | 2.96E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36918.t1 | MTR_8g032860 | 100.000 | 93 | 0 | 0 | 7 | 99 | 9 | 101 | 4.01e-64 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36918.t1 | AT2G45695 | 81.720 | 93 | 17 | 0 | 7 | 99 | 9 | 101 | 4.01e-51 | 156 |
| MS.gene36918.t1 | AT3G61113 | 83.871 | 93 | 13 | 1 | 7 | 99 | 9 | 99 | 1.55e-50 | 154 |
Find 27 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGACATTGTGAATCTTCT+TGG | 0.180525 | 8.2:-64624163 | None:intergenic |
| CCATGAAAGGATTGCTTTCT+TGG | 0.196751 | 8.2:+64624316 | MS.gene36918:CDS |
| ATCTCCTTTCATAAACATTT+CGG | 0.225395 | 8.2:-64624367 | None:intergenic |
| TCTCCTTTCATAAACATTTC+GGG | 0.253880 | 8.2:-64624366 | None:intergenic |
| GGTGCGAACCAACTTGATTA+AGG | 0.317397 | 8.2:+64624338 | MS.gene36918:CDS |
| CCAAGAAAGCAATCCTTTCA+TGG | 0.322793 | 8.2:-64624316 | None:intergenic |
| CATGAAAGGATTGCTTTCTT+GGG | 0.426218 | 8.2:+64624317 | MS.gene36918:CDS |
| TCCTTGTGAATGATTGTGAT+TGG | 0.440388 | 8.2:+64625345 | MS.gene36918:CDS |
| CCTTGTGAATGATTGTGATT+GGG | 0.497501 | 8.2:+64625346 | MS.gene36918:CDS |
| GTCTTTATATCAACCTTGCA+TGG | 0.507829 | 8.2:+64625416 | MS.gene36918:CDS |
| GGTTTGTCTTGCAGTAGACC+TGG | 0.512150 | 8.2:+64625314 | MS.gene36918:intron |
| GGGCCTCTCCTTAATCAAGT+TGG | 0.514014 | 8.2:-64624346 | None:intergenic |
| TTCACAAGGACGAGAACTCC+AGG | 0.527292 | 8.2:-64625332 | None:intergenic |
| ATGGTTGAAACATGCAGAGG+GGG | 0.600255 | 8.2:+64624120 | MS.gene36918:CDS |
| AGGCCCGAAATGTTTATGAA+AGG | 0.605720 | 8.2:+64624363 | MS.gene36918:CDS |
| CCCAATCACAATCATTCACA+AGG | 0.609803 | 8.2:-64625346 | None:intergenic |
| TAGAAGCTAACCACCATGCA+AGG | 0.612028 | 8.2:-64625429 | None:intergenic |
| AAATGGTTGAAACATGCAGA+GGG | 0.627873 | 8.2:+64624118 | None:intergenic |
| TGGTCAACTGAGTACTACAC+TGG | 0.630784 | 8.2:+64625376 | MS.gene36918:CDS |
| TTTATATCAACCTTGCATGG+TGG | 0.664106 | 8.2:+64625419 | MS.gene36918:CDS |
| ACTGGAAGAGAAAGATGTGG+TGG | 0.669266 | 8.2:+64625394 | MS.gene36918:CDS |
| GCAACAAAATAAAGAAGACA+AGG | 0.675037 | 8.2:+64624197 | MS.gene36918:CDS |
| GATTGTGATTGGGAGCTAAG+TGG | 0.687042 | 8.2:+64625356 | MS.gene36918:CDS |
| GAACCAACTTGATTAAGGAG+AGG | 0.706561 | 8.2:+64624343 | MS.gene36918:CDS |
| TACACTGGAAGAGAAAGATG+TGG | 0.715579 | 8.2:+64625391 | MS.gene36918:CDS |
| AAAATGGTTGAAACATGCAG+AGG | 0.722401 | 8.2:+64624117 | None:intergenic |
| AATGGTTGAAACATGCAGAG+GGG | 0.728461 | 8.2:+64624119 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTGTAATATTATCATTAT+AGG | + | chr8.2:64625264-64625283 | MS.gene36918:intron | 15.0% |
| !! | TGTGTAATATTATCATTATA+GGG | + | chr8.2:64625265-64625284 | MS.gene36918:intron | 15.0% |
| !! | AACCTAATATGATATTAATG+AGG | - | chr8.2:64624705-64624724 | None:intergenic | 20.0% |
| !! | ACATAAAAATAAGCTCATTT+AGG | - | chr8.2:64624455-64624474 | None:intergenic | 20.0% |
| !! | CATAAAAATAAGCTCATTTA+GGG | - | chr8.2:64624454-64624473 | None:intergenic | 20.0% |
| !! | GTATATGACTTAAAAATGAT+TGG | + | chr8.2:64624222-64624241 | MS.gene36918:intron | 20.0% |
| !!! | ATCATATTAGGTTTTTATCT+TGG | + | chr8.2:64624712-64624731 | MS.gene36918:intron | 20.0% |
| !!! | ATTTTTATGTGAGTGATTAA+TGG | + | chr8.2:64624465-64624484 | MS.gene36918:intron | 20.0% |
| !!! | TCATATTAGGTTTTTATCTT+GGG | + | chr8.2:64624713-64624732 | MS.gene36918:intron | 20.0% |
| ! | ACTAAAGAAAAACCTTTCAT+GGG | - | chr8.2:64624637-64624656 | None:intergenic | 25.0% |
| ! | ATCGGAGATATTTAATAGAA+AGG | + | chr8.2:64624658-64624677 | MS.gene36918:intron | 25.0% |
| ! | ATCTCCTTTCATAAACATTT+CGG | - | chr8.2:64624370-64624389 | None:intergenic | 25.0% |
| ! | TCAAAGTTCAAACTCTATTA+TGG | - | chr8.2:64624934-64624953 | None:intergenic | 25.0% |
| ! | TGATAAGAGTTTATAAAGAG+TGG | - | chr8.2:64624781-64624800 | None:intergenic | 25.0% |
| !! | AAAGGTTTTTCTTTAGTCAT+CGG | + | chr8.2:64624640-64624659 | MS.gene36918:intron | 25.0% |
| !! | AATCATTTAGGTTCAGATAA+TGG | - | chr8.2:64624526-64624545 | None:intergenic | 25.0% |
| !! | TATTACACATTTTATGCATC+TGG | - | chr8.2:64625254-64625273 | None:intergenic | 25.0% |
| !!! | ACTTAAAAATGATTGGTCTA+TGG | + | chr8.2:64624229-64624248 | MS.gene36918:intron | 25.0% |
| !!! | GTTTAGACAATCATTTTTCT+TGG | + | chr8.2:64624584-64624603 | MS.gene36918:intron | 25.0% |
| AAACGAAAGCTTATCTACTA+AGG | - | chr8.2:64624568-64624587 | None:intergenic | 30.0% | |
| AACGAAAGCTTATCTACTAA+GGG | - | chr8.2:64624567-64624586 | None:intergenic | 30.0% | |
| ATCTGATATCAACGTATCAA+AGG | - | chr8.2:64625214-64625233 | None:intergenic | 30.0% | |
| ATTGACATTGTGAATCTTCT+TGG | - | chr8.2:64624166-64624185 | None:intergenic | 30.0% | |
| GAACCTAAATGATTGAATGA+TGG | + | chr8.2:64624532-64624551 | MS.gene36918:intron | 30.0% | |
| GACTAAAGAAAAACCTTTCA+TGG | - | chr8.2:64624638-64624657 | None:intergenic | 30.0% | |
| GCAACAAAATAAAGAAGACA+AGG | + | chr8.2:64624197-64624216 | MS.gene36918:CDS | 30.0% | |
| GGCCTCATTAATATCATATT+AGG | + | chr8.2:64624700-64624719 | MS.gene36918:intron | 30.0% | |
| TCTCCTTTCATAAACATTTC+GGG | - | chr8.2:64624369-64624388 | None:intergenic | 30.0% | |
| TCTGTTAAAGACATATGTTC+TGG | + | chr8.2:64625132-64625151 | MS.gene36918:intron | 30.0% | |
| TGTCCATCATTCAATCATTT+AGG | - | chr8.2:64624538-64624557 | None:intergenic | 30.0% | |
| ! | AATTCAATGAGAGTACATGA+AGG | + | chr8.2:64625015-64625034 | MS.gene36918:intron | 30.0% |
| !!! | CTTTTTCAGTTAACCATGAA+AGG | + | chr8.2:64624303-64624322 | MS.gene36918:intron | 30.0% |
| AAAGAAAAACCTTTCATGGG+TGG | - | chr8.2:64624634-64624653 | None:intergenic | 35.0% | |
| AAGTCCTGCATAGAAAAGAT+AGG | - | chr8.2:64624809-64624828 | None:intergenic | 35.0% | |
| ACTTAAGATGAAATTGACCG+TGG | - | chr8.2:64624496-64624515 | None:intergenic | 35.0% | |
| ATGTCTTTAACAGACTCAAC+TGG | - | chr8.2:64625126-64625145 | None:intergenic | 35.0% | |
| CAGATTTGTAAGGATGAATC+AGG | - | chr8.2:64624739-64624758 | None:intergenic | 35.0% | |
| CATGAAAGGATTGCTTTCTT+GGG | + | chr8.2:64624317-64624336 | MS.gene36918:CDS | 35.0% | |
| CCTTGTGAATGATTGTGATT+GGG | + | chr8.2:64625346-64625365 | MS.gene36918:CDS | 35.0% | |
| CTGATTCATCCTTACAAATC+TGG | + | chr8.2:64624737-64624756 | MS.gene36918:intron | 35.0% | |
| GATGCGTGTATATAAATGGT+GGG | + | chr8.2:64624883-64624902 | MS.gene36918:intron | 35.0% | |
| GCATGATGCGTGTATATAAA+TGG | + | chr8.2:64624879-64624898 | MS.gene36918:intron | 35.0% | |
| GTCTTTATATCAACCTTGCA+TGG | + | chr8.2:64625416-64625435 | MS.gene36918:CDS | 35.0% | |
| TAACTAATGATGCATGACTC+AGG | - | chr8.2:64624421-64624440 | None:intergenic | 35.0% | |
| TCCTTGTGAATGATTGTGAT+TGG | + | chr8.2:64625345-64625364 | MS.gene36918:CDS | 35.0% | |
| TGATCAGAATGATCACCATA+AGG | - | chr8.2:64625167-64625186 | None:intergenic | 35.0% | |
| TGATGCGTGTATATAAATGG+TGG | + | chr8.2:64624882-64624901 | MS.gene36918:intron | 35.0% | |
| TTTATATCAACCTTGCATGG+TGG | + | chr8.2:64625419-64625438 | MS.gene36918:CDS | 35.0% | |
| ! | ATCTTTTCTATGCAGGACTT+GGG | + | chr8.2:64624809-64624828 | MS.gene36918:intron | 35.0% |
| ! | TATCTTTTCTATGCAGGACT+TGG | + | chr8.2:64624808-64624827 | MS.gene36918:intron | 35.0% |
| !! | GAACTATTTATTTCTGCCTG+TGG | + | chr8.2:64625293-64625312 | MS.gene36918:intron | 35.0% |
| AGGAATGCTTGTGCTTAAGT+TGG | + | chr8.2:64624678-64624697 | MS.gene36918:intron | 40.0% | |
| AGGCCCGAAATGTTTATGAA+AGG | + | chr8.2:64624363-64624382 | MS.gene36918:CDS | 40.0% | |
| AGTGATTAATGGTCTAGCCA+CGG | + | chr8.2:64624476-64624495 | MS.gene36918:intron | 40.0% | |
| CCAAGAAAGCAATCCTTTCA+TGG | - | chr8.2:64624319-64624338 | None:intergenic | 40.0% | |
| CCATGAAAGGATTGCTTTCT+TGG | + | chr8.2:64624316-64624335 | MS.gene36918:CDS | 40.0% | |
| CCCAATCACAATCATTCACA+AGG | - | chr8.2:64625349-64625368 | None:intergenic | 40.0% | |
| CCTTACAAGCCAGATTTGTA+AGG | - | chr8.2:64624749-64624768 | None:intergenic | 40.0% | |
| GAACCAACTTGATTAAGGAG+AGG | + | chr8.2:64624343-64624362 | MS.gene36918:CDS | 40.0% | |
| GGAATGCTTGTGCTTAAGTT+GGG | + | chr8.2:64624679-64624698 | MS.gene36918:intron | 40.0% | |
| GTTAACTAGACGTAGTTACG+AGG | - | chr8.2:64625062-64625081 | None:intergenic | 40.0% | |
| TACACTGGAAGAGAAAGATG+TGG | + | chr8.2:64625391-64625410 | MS.gene36918:CDS | 40.0% | |
| TGGCAACTGTCAGACATATA+GGG | - | chr8.2:64625106-64625125 | None:intergenic | 40.0% | |
| TTCTGGTGTGATACACCTTA+TGG | + | chr8.2:64625149-64625168 | MS.gene36918:intron | 40.0% | |
| ! | AAATCTGGCTTGTAAGGTGA+GGG | + | chr8.2:64624752-64624771 | MS.gene36918:intron | 40.0% |
| ! | CCTTACAAATCTGGCTTGTA+AGG | + | chr8.2:64624746-64624765 | MS.gene36918:intron | 40.0% |
| ! | GAGTCCTATCTTTTCTATGC+AGG | + | chr8.2:64624802-64624821 | MS.gene36918:intron | 40.0% |
| ACTGGAAGAGAAAGATGTGG+TGG | + | chr8.2:64625394-64625413 | MS.gene36918:CDS | 45.0% | |
| ATGGTTGAAACATGCAGAGG+GGG | + | chr8.2:64624120-64624139 | MS.gene36918:CDS | 45.0% | |
| CTGGCAACTGTCAGACATAT+AGG | - | chr8.2:64625107-64625126 | None:intergenic | 45.0% | |
| GATTGTGATTGGGAGCTAAG+TGG | + | chr8.2:64625356-64625375 | MS.gene36918:CDS | 45.0% | |
| GGTATCAGAGCCTAACATGT+CGG | - | chr8.2:64624913-64624932 | None:intergenic | 45.0% | |
| GGTGCGAACCAACTTGATTA+AGG | + | chr8.2:64624338-64624357 | MS.gene36918:CDS | 45.0% | |
| GTATCAGAGCCTAACATGTC+GGG | - | chr8.2:64624912-64624931 | None:intergenic | 45.0% | |
| TCCTCATTACCACCCATGAA+AGG | + | chr8.2:64624622-64624641 | MS.gene36918:intron | 45.0% | |
| TCTACTGCAAGACAAACCAC+AGG | - | chr8.2:64625312-64625331 | None:intergenic | 45.0% | |
| TGGTCAACTGAGTACTACAC+TGG | + | chr8.2:64625376-64625395 | MS.gene36918:CDS | 45.0% | |
| ! | AATCTGGCTTGTAAGGTGAG+GGG | + | chr8.2:64624753-64624772 | MS.gene36918:intron | 45.0% |
| ! | ACCTTTCATGGGTGGTAATG+AGG | - | chr8.2:64624626-64624645 | None:intergenic | 45.0% |
| ! | CAAATCTGGCTTGTAAGGTG+AGG | + | chr8.2:64624751-64624770 | MS.gene36918:intron | 45.0% |
| ! | GCGTGTATATAAATGGTGGG+TGG | + | chr8.2:64624886-64624905 | MS.gene36918:intron | 45.0% |
| AGGGTGTGCAGGGATGTATT+GGG | - | chr8.2:64624840-64624859 | None:intergenic | 50.0% | |
| GCATCATGCCCAATAGTGCA+GGG | - | chr8.2:64624868-64624887 | None:intergenic | 50.0% | |
| GGGCCTCTCCTTAATCAAGT+TGG | - | chr8.2:64624349-64624368 | None:intergenic | 50.0% | |
| GGTTTGTCTTGCAGTAGACC+TGG | + | chr8.2:64625314-64625333 | MS.gene36918:intron | 50.0% | |
| TGAAACATGCAGAGGGGGTT+TGG | + | chr8.2:64624125-64624144 | MS.gene36918:CDS | 50.0% | |
| TTCACAAGGACGAGAACTCC+AGG | - | chr8.2:64625335-64625354 | None:intergenic | 50.0% | |
| CAGGGTGTGCAGGGATGTAT+TGG | - | chr8.2:64624841-64624860 | None:intergenic | 55.0% | |
| CGCATCATGCCCAATAGTGC+AGG | - | chr8.2:64624869-64624888 | None:intergenic | 55.0% | |
| GGGTGTGCAGGGATGTATTG+GGG | - | chr8.2:64624839-64624858 | None:intergenic | 55.0% | |
| CCAATAGTGCAGGGCGTGCA+GGG | - | chr8.2:64624859-64624878 | None:intergenic | 60.0% | |
| CCTGCACGCCCTGCACTATT+GGG | + | chr8.2:64624857-64624876 | MS.gene36918:intron | 60.0% | |
| CCCAATAGTGCAGGGCGTGC+AGG | - | chr8.2:64624860-64624879 | None:intergenic | 65.0% | |
| CCCTGCACGCCCTGCACTAT+TGG | + | chr8.2:64624856-64624875 | MS.gene36918:intron | 65.0% | |
| GGTGGGTGGCCCGACATGTT+AGG | + | chr8.2:64624900-64624919 | MS.gene36918:intron | 65.0% | |
| CAGGGCGTGCAGGGTGTGCA+GGG | - | chr8.2:64624850-64624869 | None:intergenic | 70.0% | |
| GCAGGGCGTGCAGGGTGTGC+AGG | - | chr8.2:64624851-64624870 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 64624120 | 64625445 | 64624120 | ID=MS.gene36918 |
| chr8.2 | mRNA | 64624120 | 64625445 | 64624120 | ID=MS.gene36918.t1;Parent=MS.gene36918 |
| chr8.2 | exon | 64624120 | 64624218 | 64624120 | ID=MS.gene36918.t1.exon1;Parent=MS.gene36918.t1 |
| chr8.2 | CDS | 64624120 | 64624218 | 64624120 | ID=cds.MS.gene36918.t1;Parent=MS.gene36918.t1 |
| chr8.2 | exon | 64624312 | 64624394 | 64624312 | ID=MS.gene36918.t1.exon2;Parent=MS.gene36918.t1 |
| chr8.2 | CDS | 64624312 | 64624394 | 64624312 | ID=cds.MS.gene36918.t1;Parent=MS.gene36918.t1 |
| chr8.2 | exon | 64625328 | 64625445 | 64625328 | ID=MS.gene36918.t1.exon3;Parent=MS.gene36918.t1 |
| chr8.2 | CDS | 64625328 | 64625445 | 64625328 | ID=cds.MS.gene36918.t1;Parent=MS.gene36918.t1 |
| Gene Sequence |
| Protein sequence |
