AlfalfaGEDB | MS.gene36993

M. sativa cultivar XinJiangDaYe / MS.gene36993

KEGG: K02907
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00327.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36993.t1	XP_013444321.1	100	106	0	0	1	106	1	106	2.00E-51	211.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36993.t1	I3S856	100.0	106	0	0	1	106	1	106	1.4e-51	211.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050621	MS.gene36993	0.806739	7.11E-50	-1.69E-46
MS.gene060122	MS.gene36993	0.828322	9.59E-55	-1.69E-46
MS.gene060125	MS.gene36993	0.807498	4.91E-50	-1.69E-46
MS.gene06052	MS.gene36993	-0.80959	1.75E-50	-1.69E-46
MS.gene060932	MS.gene36993	-0.831563	1.56E-55	-1.69E-46
MS.gene06121	MS.gene36993	-0.805803	1.12E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36993.t1	MTR_8g016300	100.000	106	0	0	1	106	1	106	2.47e-76	220
MS.gene36993.t1	MTR_8g099150	98.113	106	2	0	1	106	1	106	4.01e-75	217

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36993.t1	AT5G55140	64.762	105	36	1	3	106	1	105	6.44e-46	144

Find 42 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCTAGTGAGGGGTATTC+CGG	0.253922	8.2:-76757408	MS.gene36993:CDS
CACTAGAGTTATATAAAGAT+TGG	0.372018	8.2:+76757421	None:intergenic
TGTATTTGATATAGGTTAAG+AGG	0.379656	8.2:-76757205	MS.gene36993:intron
TCAACCTGCTTCTTCTGCTT+AGG	0.384301	8.2:-76757090	None:intergenic
CAACCTGCTTCTTCTGCTTA+GGG	0.390606	8.2:-76757089	None:intergenic
CATGAGCATGAATGCGTTCA+AGG	0.398457	8.2:-76757477	MS.gene36993:CDS
GATGGAACACACCTACTGTT+AGG	0.400025	8.2:-76757316	MS.gene36993:CDS
ACTCTAGTGAGGGGTATTCC+GGG	0.407993	8.2:-76757407	MS.gene36993:CDS
AGATTGGGGCTATAAGCAAT+TGG	0.415456	8.2:+76757437	None:intergenic
ACTAGAGTTATATAAAGATT+GGG	0.420712	8.2:+76757422	None:intergenic
AAGCAGAAGAAGCAGGTTGA+TGG	0.435151	8.2:+76757093	None:intergenic
AGTGCGCCTATGTTGCTTCC+TGG	0.448328	8.2:+76757382	None:intergenic
CTATGTTGCTTCCTGGTCCC+CGG	0.462046	8.2:+76757389	None:intergenic
ATGGAACACACCTACTGTTA+GGG	0.464145	8.2:-76757315	MS.gene36993:CDS
GTACAAGGCCCGCAAAGAAA+AGG	0.493013	8.2:-76757156	MS.gene36993:CDS
GATTGGGGCTATAAGCAATT+GGG	0.504706	8.2:+76757438	None:intergenic
CGGGGACCAGGAAGCAACAT+AGG	0.508472	8.2:-76757388	MS.gene36993:CDS
TGTTCCATCGCATGACAGTT+CGG	0.511917	8.2:+76757330	None:intergenic
GAAGCAACATAGGCGCACTT+TGG	0.520220	8.2:-76757378	MS.gene36993:CDS
TGAGGGGTATTCCGGGGACC+AGG	0.527749	8.2:-76757400	MS.gene36993:CDS
CCGAGCTCTTCGTCCTCCGT+TGG	0.538228	8.2:-76757123	MS.gene36993:CDS
CGGAGGACGAAGAGCTCGGT+GGG	0.539550	8.2:+76757127	None:intergenic
CTTGAACGCATTCATGCTCA+TGG	0.548540	8.2:+76757478	None:intergenic
GTACCCTAAGCAGAAGAAGC+AGG	0.555500	8.2:+76757086	None:intergenic
GCTAGTTGTTGTTGAGACAG+AGG	0.564385	8.2:-76757183	MS.gene36993:CDS
TGTTAGGGGAATGATCCAGC+AGG	0.585091	8.2:-76757300	MS.gene36993:intron
GCTATAAGCAATTGGGACAC+AGG	0.590348	8.2:+76757445	None:intergenic
GGTTGATGGTTTATGACCAA+CGG	0.595496	8.2:+76757107	None:intergenic
TCTTTATATAACTCTAGTGA+GGG	0.599888	8.2:-76757417	MS.gene36993:CDS
GCAACCGAACTGTCATGCGA+TGG	0.602415	8.2:-76757334	MS.gene36993:CDS
CTAGAGTTATATAAAGATTG+GGG	0.610839	8.2:+76757423	None:intergenic
ATCTTTATATAACTCTAGTG+AGG	0.616129	8.2:-76757418	MS.gene36993:CDS
TGGATCATTCCCCTAACAGT+AGG	0.620579	8.2:+76757305	None:intergenic
CAAGGCCCGCAAAGAAAAGG+AGG	0.626844	8.2:-76757153	MS.gene36993:CDS
CCAACGGAGGACGAAGAGCT+CGG	0.633862	8.2:+76757123	None:intergenic
TGAGACAGAGGAGATGTACA+AGG	0.642473	8.2:-76757171	MS.gene36993:CDS
TGGAACACACCTACTGTTAG+GGG	0.654422	8.2:-76757314	MS.gene36993:CDS
CTCTAGTGAGGGGTATTCCG+GGG	0.659985	8.2:-76757406	MS.gene36993:CDS
ACTTTGGAAGCACTACGTCT+CGG	0.676793	8.2:-76757362	MS.gene36993:CDS
ACGGAGGACGAAGAGCTCGG+TGG	0.687356	8.2:+76757126	None:intergenic
CTTTATATAACTCTAGTGAG+GGG	0.705279	8.2:-76757416	MS.gene36993:CDS
TGATGGTTTATGACCAACGG+AGG	0.718299	8.2:+76757110	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTAGAGTTATATAAAGATT+GGG	+	chr8.2:76757148-76757167	None:intergenic	20.0%
!	ATCTTTATATAACTCTAGTG+AGG	-	chr8.2:76757149-76757168	MS.gene36993:CDS	25.0%
!	CACTAGAGTTATATAAAGAT+TGG	+	chr8.2:76757149-76757168	None:intergenic	25.0%
!	CTAGAGTTATATAAAGATTG+GGG	+	chr8.2:76757147-76757166	None:intergenic	25.0%
!	GCAAACTTTGTATTTGATAT+AGG	-	chr8.2:76757354-76757373	MS.gene36993:CDS	25.0%
!	TCTTTATATAACTCTAGTGA+GGG	-	chr8.2:76757150-76757169	MS.gene36993:CDS	25.0%
!	TGTATTTGATATAGGTTAAG+AGG	-	chr8.2:76757362-76757381	MS.gene36993:CDS	25.0%
	CTTTATATAACTCTAGTGAG+GGG	-	chr8.2:76757151-76757170	MS.gene36993:CDS	30.0%
!	GCAGATATGTCATTTCAAAA+TGG	+	chr8.2:76757315-76757334	None:intergenic	30.0%
	TGCCTGTATACTAACAACAT+CGG	-	chr8.2:76757332-76757351	MS.gene36993:CDS	35.0%
	AGATTGGGGCTATAAGCAAT+TGG	+	chr8.2:76757133-76757152	None:intergenic	40.0%
	ATGGAACACACCTACTGTTA+GGG	-	chr8.2:76757252-76757271	MS.gene36993:intron	40.0%
	GATTGGGGCTATAAGCAATT+GGG	+	chr8.2:76757132-76757151	None:intergenic	40.0%
	TGCCGATGTTGTTAGTATAC+AGG	+	chr8.2:76757337-76757356	None:intergenic	40.0%
!!	GGTTGATGGTTTATGACCAA+CGG	+	chr8.2:76757463-76757482	None:intergenic	40.0%
	AGAGCTATGTTGAAACCTGC+TGG	+	chr8.2:76757285-76757304	None:intergenic	45.0%
	GATGGAACACACCTACTGTT+AGG	-	chr8.2:76757251-76757270	MS.gene36993:intron	45.0%
	GCTATAAGCAATTGGGACAC+AGG	+	chr8.2:76757125-76757144	None:intergenic	45.0%
	TGAGACAGAGGAGATGTACA+AGG	-	chr8.2:76757396-76757415	MS.gene36993:CDS	45.0%
	TGATGGTTTATGACCAACGG+AGG	+	chr8.2:76757460-76757479	None:intergenic	45.0%
	TGGAACACACCTACTGTTAG+GGG	-	chr8.2:76757253-76757272	MS.gene36993:intron	45.0%
	TGGATCATTCCCCTAACAGT+AGG	+	chr8.2:76757265-76757284	None:intergenic	45.0%
	TGTTCCATCGCATGACAGTT+CGG	+	chr8.2:76757240-76757259	None:intergenic	45.0%
!	AAGCAGAAGAAGCAGGTTGA+TGG	+	chr8.2:76757477-76757496	None:intergenic	45.0%
!	GCTAGTTGTTGTTGAGACAG+AGG	-	chr8.2:76757384-76757403	MS.gene36993:CDS	45.0%
!!	AACTCTAGTGAGGGGTATTC+CGG	-	chr8.2:76757159-76757178	MS.gene36993:CDS	45.0%
!!	ACTTTGGAAGCACTACGTCT+CGG	-	chr8.2:76757205-76757224	MS.gene36993:intron	45.0%
	GAAGCAACATAGGCGCACTT+TGG	-	chr8.2:76757189-76757208	MS.gene36993:CDS	50.0%
	GTACAAGGCCCGCAAAGAAA+AGG	-	chr8.2:76757411-76757430	MS.gene36993:CDS	50.0%
	TGTTAGGGGAATGATCCAGC+AGG	-	chr8.2:76757267-76757286	MS.gene36993:intron	50.0%
!	GGCTTCCTCCTTTTCTTTGC+GGG	+	chr8.2:76757422-76757441	None:intergenic	50.0%
!	GGGCTTCCTCCTTTTCTTTG+CGG	+	chr8.2:76757423-76757442	None:intergenic	50.0%
!!	ACTCTAGTGAGGGGTATTCC+GGG	-	chr8.2:76757160-76757179	MS.gene36993:CDS	50.0%
	CAAGGCCCGCAAAGAAAAGG+AGG	-	chr8.2:76757414-76757433	MS.gene36993:CDS	55.0%
	GCAACCGAACTGTCATGCGA+TGG	-	chr8.2:76757233-76757252	MS.gene36993:intron	55.0%
!	AGTGCGCCTATGTTGCTTCC+TGG	+	chr8.2:76757188-76757207	None:intergenic	55.0%
!	CTATGTTGCTTCCTGGTCCC+CGG	+	chr8.2:76757181-76757200	None:intergenic	55.0%
!!	CTCTAGTGAGGGGTATTCCG+GGG	-	chr8.2:76757161-76757180	MS.gene36993:CDS	55.0%
	CCAACGGAGGACGAAGAGCT+CGG	+	chr8.2:76757447-76757466	None:intergenic	60.0%
	CGGGGACCAGGAAGCAACAT+AGG	-	chr8.2:76757179-76757198	MS.gene36993:CDS	60.0%
	ACGGAGGACGAAGAGCTCGG+TGG	+	chr8.2:76757444-76757463	None:intergenic	65.0%
	CCGAGCTCTTCGTCCTCCGT+TGG	-	chr8.2:76757444-76757463	MS.gene36993:CDS	65.0%
	CGGAGGACGAAGAGCTCGGT+GGG	+	chr8.2:76757443-76757462	None:intergenic	65.0%
!!	TGAGGGGTATTCCGGGGACC+AGG	-	chr8.2:76757167-76757186	MS.gene36993:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	76757091	76757498	76757091	ID=MS.gene36993
chr8.2	mRNA	76757091	76757498	76757091	ID=MS.gene36993.t1;Parent=MS.gene36993
chr8.2	exon	76757301	76757498	76757301	ID=MS.gene36993.t1.exon1;Parent=MS.gene36993.t1
chr8.2	CDS	76757301	76757498	76757301	ID=cds.MS.gene36993.t1;Parent=MS.gene36993.t1
chr8.2	exon	76757091	76757213	76757091	ID=MS.gene36993.t1.exon2;Parent=MS.gene36993.t1
chr8.2	CDS	76757091	76757213	76757091	ID=cds.MS.gene36993.t1;Parent=MS.gene36993.t1

Gene Sequence

>MS.gene36993

ATGAGCATGAATGCGTTCAAGGCATTTAAGTCCTGTGTCCCAATTGCTTATAGCCCCAATCTTTATATAACTCTAGTGAGGGGTATTCCGGGGACCAGGAAGCAACATAGGCGCACTTTGGAAGCACTACGTCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTTTCAACATAGCTCTATCAAATCCATTTTGAAATGACATATCTGCCTGTATACTAACAACATCGGCAAACTTTGTATTTGATATAGGTTAAGAGGCTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAAAAGGAGGAAGCCCACCGAGCTCTTCGTCCTCCGTTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG

Protein sequence

>MS.gene36993.t1

MSMNAFKAFKSCVPIAYSPNLYITLVRGIPGTRKQHRRTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEEAHRALRPPLVINHQPASSA