AlfalfaGEDB | MS.gene37156

M. sativa cultivar XinJiangDaYe / MS.gene37156

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37156.t1	XP_013451170.1	99.5	200	1	0	1	200	1	200	9.30E-103	382.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37156.t1	Q9FMC2	54.1	109	49	1	62	169	43	151	4.2e-19	96.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37156.t1	G7ZV06	99.5	200	1	0	1	200	1	200	6.7e-103	382.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene37156.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050444	MS.gene37156	-0.805331	1.41E-49	-1.69E-46
MS.gene050904	MS.gene37156	-0.843611	1.26E-58	-1.69E-46
MS.gene051317	MS.gene37156	-0.811219	7.81E-51	-1.69E-46
MS.gene053108	MS.gene37156	0.817992	2.47E-52	-1.69E-46
MS.gene055751	MS.gene37156	-0.805966	1.03E-49	-1.69E-46
MS.gene056387	MS.gene37156	-0.800261	1.57E-48	-1.69E-46
MS.gene056618	MS.gene37156	0.846267	2.42E-59	-1.69E-46
MS.gene059526	MS.gene37156	0.814495	1.50E-51	-1.69E-46
MS.gene06081	MS.gene37156	0.818862	1.57E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37156.t1	MTR_6g016375	99.500	200	1	0	1	200	1	200	5.81e-147	406
MS.gene37156.t1	MTR_7g017880	64.286	196	58	6	1	189	1	191	7.30e-66	201
MS.gene37156.t1	MTR_7g029400	59.627	161	56	5	9	167	8	161	1.87e-54	172
MS.gene37156.t1	MTR_8g015250	59.375	160	48	5	9	167	8	151	4.60e-54	171
MS.gene37156.t1	MTR_4g070860	52.326	86	38	2	73	158	16	98	2.37e-18	78.6
MS.gene37156.t1	MTR_1g022495	40.196	102	61	0	64	165	4	105	1.89e-17	76.3
MS.gene37156.t1	MTR_3g117120	50.000	80	40	0	79	158	19	98	4.64e-17	75.1
MS.gene37156.t1	MTR_4g097440	36.620	142	66	3	43	162	8	147	1.40e-16	74.3
MS.gene37156.t1	MTR_5g015090	41.509	106	57	1	65	165	31	136	7.10e-13	63.9
MS.gene37156.t1	MTR_1g087040	39.362	94	52	2	65	158	7	95	6.47e-12	61.2
MS.gene37156.t1	MTR_8g086020	34.375	96	63	0	63	158	8	103	9.52e-12	60.8
MS.gene37156.t1	MTR_5g028850	48.438	64	33	0	90	153	310	373	7.08e-11	60.8
MS.gene37156.t1	MTR_5g028850	41.111	90	49	2	67	153	283	371	8.95e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37156.t1	AT3G30530	74.545	110	25	2	65	171	53	162	4.96e-50	160
MS.gene37156.t1	AT5G38800	61.207	116	38	2	71	186	53	161	1.19e-41	138
MS.gene37156.t1	AT5G15830	60.909	110	43	0	57	166	41	150	5.82e-39	132
MS.gene37156.t1	AT1G13600	56.693	127	45	3	71	195	68	186	1.41e-36	126
MS.gene37156.t1	AT2G04038	64.583	96	34	0	70	165	54	149	4.07e-36	124
MS.gene37156.t1	AT1G68880	46.316	95	49	1	74	168	33	125	1.53e-21	86.3
MS.gene37156.t1	AT1G75390	47.423	97	50	1	62	158	14	109	8.62e-19	80.1
MS.gene37156.t1	AT5G60830	45.192	104	52	2	89	189	70	171	1.75e-17	77.4
MS.gene37156.t1	AT1G75390	46.237	93	49	1	62	154	14	105	3.55e-16	72.0
MS.gene37156.t1	AT2G18160	48.611	72	37	0	87	158	28	99	1.87e-15	71.2
MS.gene37156.t1	AT3G62420	44.304	79	44	0	80	158	15	93	4.69e-15	69.7
MS.gene37156.t1	AT4G34590	45.946	74	40	0	85	158	22	95	3.34e-13	65.1
MS.gene37156.t1	AT2G22850	36.522	115	61	3	70	172	106	220	3.50e-11	60.8
MS.gene37156.t1	AT2G22850	36.522	115	61	3	70	172	106	220	3.50e-11	60.8

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTTTGGTCACAAGTTCTT+TGG	0.273917	6.2:+11460941	MS.gene37156:CDS
TTAGGTAATTACCATATAAT+TGG	0.292562	6.2:-11460729	None:intergenic
GGACTAAAAGAAGGGATTAA+AGG	0.295805	6.2:-11461105	None:intergenic
TCAAGTTCAATGGATCTTCT+TGG	0.335013	6.2:+11461174	MS.gene37156:CDS
AAATTCTCAAACCAATTATA+TGG	0.356492	6.2:+11460718	MS.gene37156:CDS
ATTGTTGATGAGGGGTGTTT+AGG	0.356793	6.2:-11460747	None:intergenic
TCCTCTCATTGATGAGACTA+AGG	0.387624	6.2:-11460846	None:intergenic
AGAAACACCTTGATGAGCTT+TGG	0.415939	6.2:+11460926	MS.gene37156:CDS
GTGAGCGACGCGCTGATTCG+CGG	0.437262	6.2:-11460891	None:intergenic
TCAGCTTAAAGAAGAAGCTT+TGG	0.440839	6.2:+11461044	MS.gene37156:CDS
TGTTACTTCTCTTGTTTGCA+TGG	0.445242	6.2:-11460596	None:intergenic
GGTTAAGTTTCTCTATAAGT+TGG	0.459703	6.2:-11460981	None:intergenic
AGATAAGGTGATGTGTTTGA+TGG	0.461807	6.2:-11460640	None:intergenic
CTGCTTATGCATGATGATTG+TGG	0.470545	6.2:-11460790	None:intergenic
CAATTCCATCTCAAGTTCAA+TGG	0.471344	6.2:+11461164	MS.gene37156:CDS
GGTCACAAGTTCTTTGGCTG+AGG	0.486097	6.2:+11460947	MS.gene37156:CDS
TCATCCAAAGGACTAAAAGA+AGG	0.506277	6.2:-11461114	None:intergenic
CAAACAAGAGAAGTAACAAC+AGG	0.515554	6.2:+11460601	MS.gene37156:CDS
TGTGGACTATAGTTGAAGTC+AGG	0.518116	6.2:-11460772	None:intergenic
AAGATCCATTGAACTTGAGA+TGG	0.525838	6.2:-11461169	None:intergenic
AAGTCAGGAAATTGTTGATG+AGG	0.551877	6.2:-11460757	None:intergenic
GATAAGGTGATGTGTTTGAT+GGG	0.558687	6.2:-11460639	None:intergenic
CTTGTGACCAAAGCTCATCA+AGG	0.582135	6.2:-11460933	None:intergenic
AGTCAGGAAATTGTTGATGA+GGG	0.582558	6.2:-11460756	None:intergenic
CATCCAAAGGACTAAAAGAA+GGG	0.584492	6.2:-11461113	None:intergenic
CAGCGCGTCGCTCACGCATG+AGG	0.593315	6.2:+11460899	MS.gene37156:CDS
CTAAGATATGTATCATCCAA+AGG	0.603956	6.2:-11461126	None:intergenic
TCAATGAGAGGAAACACAGA+AGG	0.630812	6.2:+11460857	MS.gene37156:CDS
ACCTTAGTCTCATCAATGAG+AGG	0.631783	6.2:+11460845	MS.gene37156:CDS
GATCATGATTCTCAGAAACA+TGG	0.641220	6.2:-11461002	None:intergenic
GTCAGGAAATTGTTGATGAG+GGG	0.693054	6.2:-11460755	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTCTCAAACCAATTATA+TGG	+	chr6.2:11460718-11460737	MS.gene37156:CDS	20.0%
!!	TTAGGTAATTACCATATAAT+TGG	-	chr6.2:11460732-11460751	None:intergenic	20.0%
!!!	ATAATTGGTTTGAGAATTTT+TGG	-	chr6.2:11460717-11460736	None:intergenic	20.0%
!!!	TTGTTATAGTTTTGTAGATA+AGG	-	chr6.2:11460658-11460677	None:intergenic	20.0%
	CTAAGATATGTATCATCCAA+AGG	-	chr6.2:11461129-11461148	None:intergenic	30.0%
	GGTTAAGTTTCTCTATAAGT+TGG	-	chr6.2:11460984-11461003	None:intergenic	30.0%
!!!	GAATTTTTGGAGTTGATATG+AGG	-	chr6.2:11460704-11460723	None:intergenic	30.0%
	AAGATCCATTGAACTTGAGA+TGG	-	chr6.2:11461172-11461191	None:intergenic	35.0%
	AAGTCAGGAAATTGTTGATG+AGG	-	chr6.2:11460760-11460779	None:intergenic	35.0%
	AGATAAGGTGATGTGTTTGA+TGG	-	chr6.2:11460643-11460662	None:intergenic	35.0%
	CAAACAAGAGAAGTAACAAC+AGG	+	chr6.2:11460601-11460620	MS.gene37156:CDS	35.0%
	CAATTCCATCTCAAGTTCAA+TGG	+	chr6.2:11461164-11461183	MS.gene37156:CDS	35.0%
	CATCCAAAGGACTAAAAGAA+GGG	-	chr6.2:11461116-11461135	None:intergenic	35.0%
	GATAAGGTGATGTGTTTGAT+GGG	-	chr6.2:11460642-11460661	None:intergenic	35.0%
	GATCATGATTCTCAGAAACA+TGG	-	chr6.2:11461005-11461024	None:intergenic	35.0%
	GGACTAAAAGAAGGGATTAA+AGG	-	chr6.2:11461108-11461127	None:intergenic	35.0%
	TCAAGTTCAATGGATCTTCT+TGG	+	chr6.2:11461174-11461193	MS.gene37156:CDS	35.0%
	TCAGCTTAAAGAAGAAGCTT+TGG	+	chr6.2:11461044-11461063	MS.gene37156:CDS	35.0%
	TCATCCAAAGGACTAAAAGA+AGG	-	chr6.2:11461117-11461136	None:intergenic	35.0%
!	AATCCCTTCTTTTAGTCCTT+TGG	+	chr6.2:11461110-11461129	MS.gene37156:CDS	35.0%
!	AGTCAGGAAATTGTTGATGA+GGG	-	chr6.2:11460759-11460778	None:intergenic	35.0%
	ACCTTAGTCTCATCAATGAG+AGG	+	chr6.2:11460845-11460864	MS.gene37156:CDS	40.0%
	AGAAACACCTTGATGAGCTT+TGG	+	chr6.2:11460926-11460945	MS.gene37156:CDS	40.0%
	CTGCTTATGCATGATGATTG+TGG	-	chr6.2:11460793-11460812	None:intergenic	40.0%
	TCAATGAGAGGAAACACAGA+AGG	+	chr6.2:11460857-11460876	MS.gene37156:CDS	40.0%
	TCCTCTCATTGATGAGACTA+AGG	-	chr6.2:11460849-11460868	None:intergenic	40.0%
!	AGCTTTGGTCACAAGTTCTT+TGG	+	chr6.2:11460941-11460960	MS.gene37156:CDS	40.0%
!	GTCAGGAAATTGTTGATGAG+GGG	-	chr6.2:11460758-11460777	None:intergenic	40.0%
!	TGTGGACTATAGTTGAAGTC+AGG	-	chr6.2:11460775-11460794	None:intergenic	40.0%
!!	ATTGTTGATGAGGGGTGTTT+AGG	-	chr6.2:11460750-11460769	None:intergenic	40.0%
	CTTGTGACCAAAGCTCATCA+AGG	-	chr6.2:11460936-11460955	None:intergenic	45.0%
	GGTCACAAGTTCTTTGGCTG+AGG	+	chr6.2:11460947-11460966	MS.gene37156:CDS	50.0%
!!	GTGAGCGACGCGCTGATTCG+CGG	-	chr6.2:11460894-11460913	None:intergenic	65.0%
	CAGCGCGTCGCTCACGCATG+AGG	+	chr6.2:11460899-11460918	MS.gene37156:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	11460598	11461200	11460598	ID=MS.gene37156
chr6.2	mRNA	11460598	11461200	11460598	ID=MS.gene37156.t1;Parent=MS.gene37156
chr6.2	exon	11460598	11461200	11460598	ID=MS.gene37156.t1.exon1;Parent=MS.gene37156.t1
chr6.2	CDS	11460598	11461200	11460598	ID=cds.MS.gene37156.t1;Parent=MS.gene37156.t1

Gene Sequence

>MS.gene37156

ATGCAAACAAGAGAAGTAACAACAGGACTCAATTACATGCTCCCATCAAACACATCACCTTATCTACAAAACTATAACAACATGATTCAAAATAACAACATTTCCTCATATCAACTCCAAAAATTCTCAAACCAATTATATGGTAATTACCTAAACACCCCTCATCAACAATTTCCTGACTTCAACTATAGTCCACAATCATCATGCATAAGCAGCAACTCAACTTCTGATGAAGCAGATGAACAAAACCTTAGTCTCATCAATGAGAGGAAACACAGAAGGATGATATCAAACCGCGAATCAGCGCGTCGCTCACGCATGAGGAAGCAGAAACACCTTGATGAGCTTTGGTCACAAGTTCTTTGGCTGAGGAATGAAAATCACCAACTTATAGAGAAACTTAACCATGTTTCTGAGAATCATGATCAAGTTGTTCAAGAGAATGCTCAGCTTAAAGAAGAAGCTTTGGAACTTCGACAAATGATAAAAGATATGCAGATTCATAGTCCTTTAATCCCTTCTTTTAGTCCTTTGGATGATACATATCTTAGAGATGATTCTTCAAACAATTCCATCTCAAGTTCAATGGATCTTCTTGGCTGA

Protein sequence

>MS.gene37156.t1

MQTREVTTGLNYMLPSNTSPYLQNYNNMIQNNNISSYQLQKFSNQLYGNYLNTPHQQFPDFNYSPQSSCISSNSTSDEADEQNLSLINERKHRRMISNRESARRSRMRKQKHLDELWSQVLWLRNENHQLIEKLNHVSENHDQVVQENAQLKEEALELRQMIKDMQIHSPLIPSFSPLDDTYLRDDSSNNSISSSMDLLG