Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37699.t1 | XP_013469751.1 | 96.2 | 186 | 5 | 2 | 1 | 184 | 1 | 186 | 9.50E-86 | 326.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37699.t1 | P11432 | 74.0 | 196 | 39 | 1 | 1 | 184 | 1 | 196 | 1.3e-70 | 267.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37699.t1 | A0A072VP35 | 96.2 | 186 | 5 | 2 | 1 | 184 | 1 | 186 | 6.8e-86 | 326.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05081 | MS.gene37699 | 0.823605 | 1.27E-53 | -1.69E-46 |
| MS.gene051734 | MS.gene37699 | 0.838506 | 2.76E-57 | -1.69E-46 |
| MS.gene051874 | MS.gene37699 | 0.806135 | 9.53E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene37699 | 0.833455 | 5.28E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene37699 | 0.857084 | 2.08E-62 | -1.69E-46 |
| MS.gene052872 | MS.gene37699 | 0.852771 | 3.71E-61 | -1.69E-46 |
| MS.gene053449 | MS.gene37699 | 0.806213 | 9.18E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene37699 | 0.821414 | 4.09E-53 | -1.69E-46 |
| MS.gene05494 | MS.gene37699 | 0.833604 | 4.85E-56 | -1.69E-46 |
| MS.gene055224 | MS.gene37699 | 0.817757 | 2.79E-52 | -1.69E-46 |
| MS.gene055539 | MS.gene37699 | 0.818059 | 2.39E-52 | -1.69E-46 |
| MS.gene055741 | MS.gene37699 | 0.808099 | 3.66E-50 | -1.69E-46 |
| MS.gene055744 | MS.gene37699 | 0.810234 | 1.28E-50 | -1.69E-46 |
| MS.gene057019 | MS.gene37699 | 0.807485 | 4.94E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene37699 | 0.803732 | 3.03E-49 | -1.69E-46 |
| MS.gene059717 | MS.gene37699 | 0.83179 | 1.37E-55 | -1.69E-46 |
| MS.gene061231 | MS.gene37699 | 0.808192 | 3.50E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37699.t1 | MTR_1g102830 | 96.237 | 186 | 5 | 2 | 1 | 184 | 1 | 186 | 8.73e-126 | 352 |
| MS.gene37699.t1 | MTR_1g102810 | 93.491 | 169 | 10 | 1 | 17 | 184 | 14 | 182 | 5.72e-113 | 319 |
| MS.gene37699.t1 | MTR_1g102780 | 81.111 | 180 | 19 | 1 | 20 | 184 | 21 | 200 | 1.04e-98 | 284 |
| MS.gene37699.t1 | MTR_1g102570 | 79.775 | 178 | 23 | 1 | 20 | 184 | 21 | 198 | 3.27e-98 | 282 |
| MS.gene37699.t1 | MTR_1g102800 | 80.203 | 197 | 26 | 1 | 1 | 184 | 1 | 197 | 5.28e-98 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37699.t1 | AT3G22840 | 50.000 | 192 | 77 | 3 | 7 | 184 | 9 | 195 | 3.47e-58 | 181 |
| MS.gene37699.t1 | AT4G14690 | 50.521 | 192 | 74 | 4 | 11 | 184 | 5 | 193 | 1.19e-53 | 169 |
Find 46 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTCCATTGATTCTTTC+AGG | 0.165068 | 1.1:-73790766 | None:intergenic |
| TAGAAGGGATGTTGTTAAAT+TGG | 0.226805 | 1.1:-73790223 | None:intergenic |
| GTTGTTGACAGTTGCTTCTT+TGG | 0.263072 | 1.1:+73790897 | MS.gene37699:CDS |
| ACACTAACACCTTCAAATAA+TGG | 0.346554 | 1.1:-73790922 | None:intergenic |
| ATTCCAAAGCTCTGCATTTG+AGG | 0.355412 | 1.1:-73790971 | None:intergenic |
| GGTTTGTTCGATCAAATATC+TGG | 0.369233 | 1.1:+73790841 | MS.gene37699:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1.1:+73791005 | MS.gene37699:CDS |
| TAGTTTGAAAGTTCGATCTA+TGG | 0.378474 | 1.1:+73790274 | MS.gene37699:CDS |
| AAGCTAGGCATATACATAGA+AGG | 0.387722 | 1.1:-73790239 | None:intergenic |
| GGCTGTCACAGAATATGTTA+AGG | 0.401224 | 1.1:+73791026 | MS.gene37699:CDS |
| TCTTTGGTTCCATTATTTGA+AGG | 0.404737 | 1.1:+73790913 | MS.gene37699:CDS |
| AATGGAAGGTTAGCCATGAT+TGG | 0.409893 | 1.1:+73790778 | MS.gene37699:CDS |
| CGGATTTGATGGCATTTGGT+GGG | 0.428133 | 1.1:+73790737 | MS.gene37699:CDS |
| GCAGGCTGAGGAGTCACCTT+TGG | 0.441538 | 1.1:-73790541 | None:intergenic |
| TCTAGGCCTGCTTGAAATGC+TGG | 0.450968 | 1.1:-73790195 | None:intergenic |
| TTGTTAAATTGGTTAACTCT+AGG | 0.463045 | 1.1:-73790212 | None:intergenic |
| TGTCCTCAAATGCAGAGCTT+TGG | 0.467503 | 1.1:+73790968 | MS.gene37699:CDS |
| GCTGTCACAGAATATGTTAA+GGG | 0.470370 | 1.1:+73791027 | MS.gene37699:CDS |
| AGTGTTGAATCTAAGTCAAA+AGG | 0.480583 | 1.1:+73790940 | MS.gene37699:CDS |
| TCGGATTTGATGGCATTTGG+TGG | 0.487791 | 1.1:+73790736 | MS.gene37699:CDS |
| TGCAGATACAAAACCAATCA+TGG | 0.488652 | 1.1:-73790791 | None:intergenic |
| GAATGGAAGACTTGCTATGT+TGG | 0.490221 | 1.1:+73790990 | MS.gene37699:CDS |
| AATGGAAGACTTGCTATGTT+GGG | 0.502682 | 1.1:+73790991 | MS.gene37699:CDS |
| TTCGATCAAATATCTGGTGG+TGG | 0.522060 | 1.1:+73790847 | MS.gene37699:CDS |
| CTAGCATTCCTTCTGAAGCT+AGG | 0.539206 | 1.1:-73790254 | None:intergenic |
| CCTGCTTGAAATGCTGGTCA+TGG | 0.548863 | 1.1:-73790189 | None:intergenic |
| GCAGCGCCTGAAAGAATCAA+TGG | 0.552540 | 1.1:+73790760 | MS.gene37699:CDS |
| CACTTGTATACAAGAACCAT+GGG | 0.556587 | 1.1:-73790873 | None:intergenic |
| GGATTTGATGGCATTTGGTG+GGG | 0.562192 | 1.1:+73790738 | MS.gene37699:CDS |
| AGCTAGGCATATACATAGAA+GGG | 0.567045 | 1.1:-73790238 | None:intergenic |
| GAACGAGTAGAAGCAGGCTG+AGG | 0.574059 | 1.1:-73790553 | None:intergenic |
| TCAAATGCAGAGCTTTGGAA+TGG | 0.580798 | 1.1:+73790973 | MS.gene37699:CDS |
| TTTGGTGAACGAGTAGAAGC+AGG | 0.581281 | 1.1:-73790559 | None:intergenic |
| TGCTTCTACTCGTTCACCAA+AGG | 0.583895 | 1.1:+73790561 | MS.gene37699:CDS |
| GGTGGAATTAGCAAAAGGGC+AGG | 0.590541 | 1.1:+73790819 | MS.gene37699:CDS |
| GAAAGTTCGATCTATGGCTG+AGG | 0.590785 | 1.1:+73790280 | MS.gene37699:CDS |
| TTGTTCGATCAAATATCTGG+TGG | 0.594040 | 1.1:+73790844 | MS.gene37699:CDS |
| TGTATATGCCTAGCTTCAGA+AGG | 0.603350 | 1.1:+73790246 | MS.gene37699:CDS |
| CCATGACCAGCATTTCAAGC+AGG | 0.603962 | 1.1:+73790189 | MS.gene37699:CDS |
| GTGGAATTAGCAAAAGGGCA+GGG | 0.608172 | 1.1:+73790820 | MS.gene37699:CDS |
| ACACTTGTATACAAGAACCA+TGG | 0.618963 | 1.1:-73790874 | None:intergenic |
| CGCCTGAAAGAATCAATGGA+AGG | 0.624472 | 1.1:+73790764 | MS.gene37699:CDS |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.1:+73790525 | MS.gene37699:CDS |
| TATAAAACTCTACTTACACA+AGG | 0.652785 | 1.1:-73791056 | None:intergenic |
| TATCTGGTGGTGGAATCCCA+TGG | 0.688884 | 1.1:+73790857 | MS.gene37699:CDS |
| ATAAAACTCTACTTACACAA+GGG | 0.707854 | 1.1:-73791055 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTTCTTTACATTATTC+AGG | + | chr1.1:73790501-73790520 | MS.gene37699:intron | 20.0% |
| !! | ATCATCATTATTCATCAAAA+AGG | - | chr1.1:73790688-73790707 | None:intergenic | 20.0% |
| !! | GGTTTATCAAACAAATATTA+AGG | + | chr1.1:73790310-73790329 | MS.gene37699:intron | 20.0% |
| ! | TTCTTTACATTATTCAGGAA+GGG | + | chr1.1:73790506-73790525 | MS.gene37699:intron | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | - | chr1.1:73790215-73790234 | None:intergenic | 25.0% |
| ! | TTTATTATGATGCTTACCTT+TGG | - | chr1.1:73790580-73790599 | None:intergenic | 25.0% |
| ! | TTTCTTTACATTATTCAGGA+AGG | + | chr1.1:73790505-73790524 | MS.gene37699:intron | 25.0% |
| !!! | GTAGTTGATTTTGTAAATTC+CGG | + | chr1.1:73790439-73790458 | MS.gene37699:intron | 25.0% |
| !!! | GTCATGTAAAGAATTGATTT+TGG | + | chr1.1:73790647-73790666 | MS.gene37699:intron | 25.0% |
| ACACTAACACCTTCAAATAA+TGG | - | chr1.1:73790925-73790944 | None:intergenic | 30.0% | |
| TAGAAGGGATGTTGTTAAAT+TGG | - | chr1.1:73790226-73790245 | None:intergenic | 30.0% | |
| TAGTTTGAAAGTTCGATCTA+TGG | + | chr1.1:73790274-73790293 | MS.gene37699:CDS | 30.0% | |
| TTACATGACTATACTCTTCT+TGG | - | chr1.1:73790636-73790655 | None:intergenic | 30.0% | |
| ! | AGTGTTGAATCTAAGTCAAA+AGG | + | chr1.1:73790940-73790959 | MS.gene37699:CDS | 30.0% |
| ! | CACAAAATTTTCGGATTTGA+TGG | + | chr1.1:73790726-73790745 | MS.gene37699:CDS | 30.0% |
| ! | GCAGATAAGCACAAAATTTT+CGG | + | chr1.1:73790717-73790736 | MS.gene37699:intron | 30.0% |
| ! | TCTTTGGTTCCATTATTTGA+AGG | + | chr1.1:73790913-73790932 | MS.gene37699:CDS | 30.0% |
| !!! | ATTGGTTTTGTATCTGCAAT+GGG | + | chr1.1:73790796-73790815 | MS.gene37699:CDS | 30.0% |
| AAGCTAGGCATATACATAGA+AGG | - | chr1.1:73790242-73790261 | None:intergenic | 35.0% | |
| AAGTGACATTCAAGACGTTA+TGG | - | chr1.1:73790470-73790489 | None:intergenic | 35.0% | |
| ACACTTGTATACAAGAACCA+TGG | - | chr1.1:73790877-73790896 | None:intergenic | 35.0% | |
| AGCTAGGCATATACATAGAA+GGG | - | chr1.1:73790241-73790260 | None:intergenic | 35.0% | |
| ATCTATGGCTGAGGTAATTA+AGG | + | chr1.1:73790289-73790308 | MS.gene37699:intron | 35.0% | |
| CACTTGTATACAAGAACCAT+GGG | - | chr1.1:73790876-73790895 | None:intergenic | 35.0% | |
| GCTGTCACAGAATATGTTAA+GGG | + | chr1.1:73791027-73791046 | MS.gene37699:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.1:73791005-73791024 | MS.gene37699:CDS | 35.0% | |
| TGCAGATACAAAACCAATCA+TGG | - | chr1.1:73790794-73790813 | None:intergenic | 35.0% | |
| TTGTTCGATCAAATATCTGG+TGG | + | chr1.1:73790844-73790863 | MS.gene37699:CDS | 35.0% | |
| ! | ACTCTTCTTGGACACAATTT+TGG | - | chr1.1:73790624-73790643 | None:intergenic | 35.0% |
| ! | GGTTTGTTCGATCAAATATC+TGG | + | chr1.1:73790841-73790860 | MS.gene37699:CDS | 35.0% |
| !! | AACCTTCCATTGATTCTTTC+AGG | - | chr1.1:73790769-73790788 | None:intergenic | 35.0% |
| !! | AATGGAAGACTTGCTATGTT+GGG | + | chr1.1:73790991-73791010 | MS.gene37699:CDS | 35.0% |
| !! | TTTTCGGATTTGATGGCATT+TGG | + | chr1.1:73790733-73790752 | MS.gene37699:CDS | 35.0% |
| !!! | GATTGGTTTTGTATCTGCAA+TGG | + | chr1.1:73790795-73790814 | MS.gene37699:CDS | 35.0% |
| !!! | TTGGTTTTGTATCTGCAATG+GGG | + | chr1.1:73790797-73790816 | MS.gene37699:CDS | 35.0% |
| ATTCCAAAGCTCTGCATTTG+AGG | - | chr1.1:73790974-73790993 | None:intergenic | 40.0% | |
| GGCTGTCACAGAATATGTTA+AGG | + | chr1.1:73791026-73791045 | MS.gene37699:CDS | 40.0% | |
| GTTGTTGACAGTTGCTTCTT+TGG | + | chr1.1:73790897-73790916 | MS.gene37699:CDS | 40.0% | |
| TCAAGACGTTATGGATTCTC+CGG | - | chr1.1:73790461-73790480 | None:intergenic | 40.0% | |
| TGTATATGCCTAGCTTCAGA+AGG | + | chr1.1:73790246-73790265 | MS.gene37699:CDS | 40.0% | |
| TTCGATCAAATATCTGGTGG+TGG | + | chr1.1:73790847-73790866 | MS.gene37699:CDS | 40.0% | |
| ! | AATGGAAGGTTAGCCATGAT+TGG | + | chr1.1:73790778-73790797 | MS.gene37699:CDS | 40.0% |
| !! | GAATGGAAGACTTGCTATGT+TGG | + | chr1.1:73790990-73791009 | MS.gene37699:CDS | 40.0% |
| !! | TCAAATGCAGAGCTTTGGAA+TGG | + | chr1.1:73790973-73790992 | MS.gene37699:CDS | 40.0% |
| !!! | TGGTTTTGTATCTGCAATGG+GGG | + | chr1.1:73790798-73790817 | MS.gene37699:CDS | 40.0% |
| ATGGGGGTGGAATTAGCAAA+AGG | + | chr1.1:73790814-73790833 | MS.gene37699:CDS | 45.0% | |
| CGCCTGAAAGAATCAATGGA+AGG | + | chr1.1:73790764-73790783 | MS.gene37699:CDS | 45.0% | |
| GAAAGTTCGATCTATGGCTG+AGG | + | chr1.1:73790280-73790299 | MS.gene37699:CDS | 45.0% | |
| GTGGAATTAGCAAAAGGGCA+GGG | + | chr1.1:73790820-73790839 | MS.gene37699:CDS | 45.0% | |
| TGCTTCTACTCGTTCACCAA+AGG | + | chr1.1:73790561-73790580 | MS.gene37699:CDS | 45.0% | |
| TGGGGGTGGAATTAGCAAAA+GGG | + | chr1.1:73790815-73790834 | MS.gene37699:CDS | 45.0% | |
| TGTCCTCAAATGCAGAGCTT+TGG | + | chr1.1:73790968-73790987 | MS.gene37699:CDS | 45.0% | |
| TTTGGTGAACGAGTAGAAGC+AGG | - | chr1.1:73790562-73790581 | None:intergenic | 45.0% | |
| ! | CGGATTTGATGGCATTTGGT+GGG | + | chr1.1:73790737-73790756 | MS.gene37699:CDS | 45.0% |
| ! | GGATTTGATGGCATTTGGTG+GGG | + | chr1.1:73790738-73790757 | MS.gene37699:CDS | 45.0% |
| ! | TCGGATTTGATGGCATTTGG+TGG | + | chr1.1:73790736-73790755 | MS.gene37699:CDS | 45.0% |
| ! | TTTTGTATCTGCAATGGGGG+TGG | + | chr1.1:73790801-73790820 | MS.gene37699:CDS | 45.0% |
| !! | CTAGCATTCCTTCTGAAGCT+AGG | - | chr1.1:73790257-73790276 | None:intergenic | 45.0% |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.1:73790525-73790544 | MS.gene37699:CDS | 50.0% | |
| CCATGACCAGCATTTCAAGC+AGG | + | chr1.1:73790189-73790208 | MS.gene37699:CDS | 50.0% | |
| CCTGCTTGAAATGCTGGTCA+TGG | - | chr1.1:73790192-73790211 | None:intergenic | 50.0% | |
| GCAGCGCCTGAAAGAATCAA+TGG | + | chr1.1:73790760-73790779 | MS.gene37699:CDS | 50.0% | |
| GGTGGAATTAGCAAAAGGGC+AGG | + | chr1.1:73790819-73790838 | MS.gene37699:CDS | 50.0% | |
| TCTAGGCCTGCTTGAAATGC+TGG | - | chr1.1:73790198-73790217 | None:intergenic | 50.0% | |
| !! | TATCTGGTGGTGGAATCCCA+TGG | + | chr1.1:73790857-73790876 | MS.gene37699:CDS | 50.0% |
| GAACGAGTAGAAGCAGGCTG+AGG | - | chr1.1:73790556-73790575 | None:intergenic | 55.0% | |
| GCAGGCTGAGGAGTCACCTT+TGG | - | chr1.1:73790544-73790563 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 73790152 | 73791065 | 73790152 | ID=MS.gene37699 |
| chr1.1 | mRNA | 73790152 | 73791065 | 73790152 | ID=MS.gene37699.t1;Parent=MS.gene37699 |
| chr1.1 | exon | 73790152 | 73790301 | 73790152 | ID=MS.gene37699.t1.exon1;Parent=MS.gene37699.t1 |
| chr1.1 | CDS | 73790152 | 73790301 | 73790152 | ID=cds.MS.gene37699.t1;Parent=MS.gene37699.t1 |
| chr1.1 | exon | 73790523 | 73790582 | 73790523 | ID=MS.gene37699.t1.exon2;Parent=MS.gene37699.t1 |
| chr1.1 | CDS | 73790523 | 73790582 | 73790523 | ID=cds.MS.gene37699.t1;Parent=MS.gene37699.t1 |
| chr1.1 | exon | 73790721 | 73791065 | 73790721 | ID=MS.gene37699.t1.exon3;Parent=MS.gene37699.t1 |
| chr1.1 | CDS | 73790721 | 73791065 | 73790721 | ID=cds.MS.gene37699.t1;Parent=MS.gene37699.t1 |
| Gene Sequence |
| Protein sequence |
