AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37934


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37934.t1 MTR_5g095470 98.442 321 5 0 1 321 1 321 0.0 649
MS.gene37934.t1 MTR_3g058410 71.111 315 81 2 17 321 22 336 1.48e-159 449
MS.gene37934.t1 MTR_8g028040 60.839 286 108 1 36 321 17 298 6.02e-125 360
MS.gene37934.t1 MTR_5g023760 48.243 313 151 4 13 320 8 314 1.67e-95 286
MS.gene37934.t1 MTR_5g023760 49.007 302 144 3 13 309 8 304 8.45e-95 284
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37934.t1 AT3G13110 64.308 325 104 3 5 321 71 391 5.48e-147 419
MS.gene37934.t1 AT1G55920 66.890 299 91 1 23 321 24 314 1.54e-145 412
MS.gene37934.t1 AT5G56760 61.567 268 102 1 55 321 45 312 4.81e-115 335
MS.gene37934.t1 AT4G35640 50.495 303 142 3 18 320 38 332 1.50e-94 285
MS.gene37934.t1 AT2G17640 54.104 268 117 2 53 320 34 295 2.07e-91 275

Find 70 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCGCGGGAACTTGTATTT+TGG 0.185561 5.1:-76282287 MS.gene37934:CDS
GGAATCTTATCAAGTTTAAT+AGG 0.262166 5.1:+76282145 None:intergenic
GTTGGTGAAACTGCTGTTAT+TGG 0.286982 5.1:-76282403 MS.gene37934:CDS
AGCACCCCGTCACCAATCTT+TGG 0.296687 5.1:+76282313 None:intergenic
ATTGGTGACGGGGTGCTTAT+AGG 0.319174 5.1:-76282307 MS.gene37934:CDS
CAAGGTGTTGATCCCATATC+TGG 0.330087 5.1:+76282942 None:intergenic
CCTATCGCCATGTGCCTTAC+CGG 0.332998 5.1:+76282339 None:intergenic
TTCACACCAAGTTCCAGATA+TGG 0.353879 5.1:-76282955 MS.gene37934:CDS
AATCTTGTCACAGAAATCAT+TGG 0.355787 5.1:-76282791 MS.gene37934:CDS
TAATAGTAACTAGACAAAAT+TGG 0.360866 5.1:+76282820 None:intergenic
TTCTTTGAATTAGCATCAAC+AGG 0.364623 5.1:+76283015 None:intergenic
ATTGGTGATGGTGCTAAAAT+TGG 0.373817 5.1:-76282253 MS.gene37934:CDS
GAAATCATTGGAAACTGCTT+TGG 0.380279 5.1:-76282779 MS.gene37934:CDS
GACTACCGTTGTTGGGAATC+CGG 0.396009 5.1:-76282194 MS.gene37934:CDS
TTTGGGGTTGATATTCATCC+TGG 0.412211 5.1:-76282475 MS.gene37934:CDS
CATTGTTTCTTGAATTTCAA+AGG 0.417026 5.1:-76282595 MS.gene37934:CDS
GGGAATCCGGCTAAATTGAT+TGG 0.417683 5.1:-76282181 MS.gene37934:CDS
GCTCATAATTTGTGGTCAAA+TGG 0.421298 5.1:-76282544 MS.gene37934:CDS
GCACCCCGTCACCAATCTTT+GGG 0.424633 5.1:+76282314 None:intergenic
TATGATTCAGAATCGCGTTT+CGG 0.428319 5.1:-76282503 MS.gene37934:CDS
TAAGATTCCTAGCCACACTA+TGG 0.429403 5.1:-76282131 MS.gene37934:CDS
CTCCAAGGACTACCGTTGTT+GGG 0.430566 5.1:-76282201 MS.gene37934:CDS
TCACACCAAGTTCCAGATAT+GGG 0.433106 5.1:-76282954 MS.gene37934:CDS
CCTCCAAGGACTACCGTTGT+TGG 0.444028 5.1:-76282202 MS.gene37934:CDS
GGCGATAGGCACCCAAAGAT+TGG 0.446766 5.1:-76282325 MS.gene37934:CDS
TTCCCTCCAATCAATTTAGC+CGG 0.459568 5.1:+76282175 None:intergenic
TTCCCAACAACGGTAGTCCT+TGG 0.463382 5.1:+76282199 None:intergenic
AGTTGTGGAACAATCTGAAA+AGG 0.473399 5.1:-76282908 MS.gene37934:CDS
ATTCATCCTGGAGCTAAGAT+TGG 0.476533 5.1:-76282463 MS.gene37934:CDS
TGCTGGTTCAGTTGTGATCA+AGG 0.485621 5.1:-76282230 MS.gene37934:CDS
AGATCAGAAAGTGTTGTACT+AGG 0.509118 5.1:+76282718 None:intergenic
ATAGGGTAGCTCATAATTTG+TGG 0.510040 5.1:-76282552 MS.gene37934:CDS
CCACTTCCAATCTTAGCTCC+AGG 0.510730 5.1:+76282457 None:intergenic
CCTGGAGCTAAGATTGGAAG+TGG 0.517686 5.1:-76282457 MS.gene37934:CDS
TTGCACGGTGTGACGTTGGG+AGG 0.518697 5.1:-76282364 MS.gene37934:CDS
TTTAGCATGTCAAGCTCATA+GGG 0.523451 5.1:-76282569 MS.gene37934:CDS
GGTGTGACGTTGGGAGGGAC+CGG 0.524462 5.1:-76282358 MS.gene37934:CDS
GGGAATATTAAGATTGGTGA+TGG 0.534999 5.1:-76282265 MS.gene37934:CDS
TGAACATGACTTATACAAGC+AGG 0.543036 5.1:+76282616 None:intergenic
GATGGTGCTAAAATTGGTGC+TGG 0.548397 5.1:-76282247 MS.gene37934:CDS
GGCACCCAAAGATTGGTGAC+GGG 0.548991 5.1:-76282318 MS.gene37934:CDS
TGATCATGCAACTGGGATTG+TGG 0.549413 5.1:-76282425 MS.gene37934:CDS
CACTGCTTAATTTAACAGCA+AGG 0.553156 5.1:+76282749 None:intergenic
ATAATTTGTGGTCAAATGGA+AGG 0.554078 5.1:-76282540 MS.gene37934:CDS
TTGATCCCATATCTGGAACT+TGG 0.557376 5.1:+76282949 None:intergenic
CACAACTGAACTTGTATGCA+AGG 0.562621 5.1:+76282924 None:intergenic
GGAGGGACCGGTAAGGCACA+TGG 0.563182 5.1:-76282346 MS.gene37934:CDS
TGCACGGTGTGACGTTGGGA+GGG 0.567057 5.1:-76282363 MS.gene37934:CDS
GACGGGGTGCTTATAGGCGC+GGG 0.567274 5.1:-76282301 MS.gene37934:CDS
CCGGTAAGGCACATGGCGAT+AGG 0.568492 5.1:-76282339 MS.gene37934:CDS
CCAACAACGGTAGTCCTTGG+AGG 0.574914 5.1:+76282202 None:intergenic
CTTGCATACAAGTTCAGTTG+TGG 0.582578 5.1:-76282923 MS.gene37934:CDS
AATCCGGCTAAATTGATTGG+AGG 0.584845 5.1:-76282178 MS.gene37934:CDS
AGGCACCCAAAGATTGGTGA+CGG 0.586567 5.1:-76282319 MS.gene37934:CDS
TTATGTGTGGATGAAGATGC+AGG 0.590043 5.1:-76282872 MS.gene37934:CDS
TGACGGGGTGCTTATAGGCG+CGG 0.592802 5.1:-76282302 MS.gene37934:CDS
ACATGATCCATAGTGTGGCT+AGG 0.594971 5.1:+76282124 None:intergenic
GGAACAATCTGAAAAGGTTG+AGG 0.607641 5.1:-76282902 MS.gene37934:CDS
CATGCAACTGGGATTGTGGT+TGG 0.611902 5.1:-76282421 MS.gene37934:CDS
GACGTTGGGAGGGACCGGTA+AGG 0.614643 5.1:-76282353 MS.gene37934:CDS
ATCCGGCTAAATTGATTGGA+GGG 0.620898 5.1:-76282177 MS.gene37934:CDS
TCACATGAATGATCATCATG+TGG 0.621543 5.1:+76282985 None:intergenic
GAACATGACTTATACAAGCA+GGG 0.643802 5.1:+76282617 None:intergenic
GATAACATAATCATAAAACT+CGG 0.645462 5.1:+76282090 None:intergenic
TGATCAAGGAAGTTCCTCCA+AGG 0.647904 5.1:-76282216 MS.gene37934:CDS
TTGAGGATGAAGATTATGTG+TGG 0.651581 5.1:-76282885 MS.gene37934:CDS
GCACCCAAAGATTGGTGACG+GGG 0.678109 5.1:-76282317 MS.gene37934:CDS
CTGGAGCTAAGATTGGAAGT+GGG 0.679499 5.1:-76282456 MS.gene37934:CDS
GTGCAACATGATCCATAGTG+TGG 0.717179 5.1:+76282119 None:intergenic
TTTAGCCGGATTCCCAACAA+CGG 0.717753 5.1:+76282189 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAACATAATCATAAAACT+CGG + chr5.1:76283030-76283049 None:intergenic 20.0%
!! TAATAGTAACTAGACAAAAT+TGG + chr5.1:76282300-76282319 None:intergenic 20.0%
!!! TGATCTTTTTGTTAGAATTT+TGG - chr5.1:76282416-76282435 MS.gene37934:CDS 20.0%
! GGAATCTTATCAAGTTTAAT+AGG + chr5.1:76282975-76282994 None:intergenic 25.0%
!! CATTGTTTCTTGAATTTCAA+AGG - chr5.1:76282522-76282541 MS.gene37934:CDS 25.0%
!!! ATTTTGGGGAATATTAAGAT+TGG - chr5.1:76282846-76282865 MS.gene37934:CDS 25.0%
AATCTTGTCACAGAAATCAT+TGG - chr5.1:76282326-76282345 MS.gene37934:CDS 30.0%
TTCTTTGAATTAGCATCAAC+AGG + chr5.1:76282105-76282124 None:intergenic 30.0%
! AATGATGTGTCTATTTTGCA+CGG - chr5.1:76282738-76282757 MS.gene37934:CDS 30.0%
! ATAATTTGTGGTCAAATGGA+AGG - chr5.1:76282577-76282596 MS.gene37934:CDS 30.0%
AGTTGTGGAACAATCTGAAA+AGG - chr5.1:76282209-76282228 MS.gene37934:CDS 35.0%
ATAGGGTAGCTCATAATTTG+TGG - chr5.1:76282565-76282584 MS.gene37934:CDS 35.0%
CACTGCTTAATTTAACAGCA+AGG + chr5.1:76282371-76282390 None:intergenic 35.0%
GAAATCATTGGAAACTGCTT+TGG - chr5.1:76282338-76282357 MS.gene37934:CDS 35.0%
GAACATGACTTATACAAGCA+GGG + chr5.1:76282503-76282522 None:intergenic 35.0%
GGGAATATTAAGATTGGTGA+TGG - chr5.1:76282852-76282871 MS.gene37934:CDS 35.0%
TATGATTCAGAATCGCGTTT+CGG - chr5.1:76282614-76282633 MS.gene37934:CDS 35.0%
TCACATGAATGATCATCATG+TGG + chr5.1:76282135-76282154 None:intergenic 35.0%
TGAACATGACTTATACAAGC+AGG + chr5.1:76282504-76282523 None:intergenic 35.0%
TTGAGGATGAAGATTATGTG+TGG - chr5.1:76282232-76282251 MS.gene37934:CDS 35.0%
TTTAGCATGTCAAGCTCATA+GGG - chr5.1:76282548-76282567 MS.gene37934:CDS 35.0%
! ATTTTGCTTGATCATGCAAC+TGG - chr5.1:76282684-76282703 MS.gene37934:CDS 35.0%
! GCTCATAATTTGTGGTCAAA+TGG - chr5.1:76282573-76282592 MS.gene37934:CDS 35.0%
! TTTTAGCATGTCAAGCTCAT+AGG - chr5.1:76282547-76282566 MS.gene37934:CDS 35.0%
! TTTTGCTTGATCATGCAACT+GGG - chr5.1:76282685-76282704 MS.gene37934:CDS 35.0%
!! AGATCAGAAAGTGTTGTACT+AGG + chr5.1:76282402-76282421 None:intergenic 35.0%
!! ATTGGTGATGGTGCTAAAAT+TGG - chr5.1:76282864-76282883 MS.gene37934:CDS 35.0%
AATCCGGCTAAATTGATTGG+AGG - chr5.1:76282939-76282958 MS.gene37934:CDS 40.0%
ATCCGGCTAAATTGATTGGA+GGG - chr5.1:76282940-76282959 MS.gene37934:CDS 40.0%
ATTCATCCTGGAGCTAAGAT+TGG - chr5.1:76282654-76282673 MS.gene37934:CDS 40.0%
CACAACTGAACTTGTATGCA+AGG + chr5.1:76282196-76282215 None:intergenic 40.0%
CTTGCATACAAGTTCAGTTG+TGG - chr5.1:76282194-76282213 MS.gene37934:CDS 40.0%
GGAACAATCTGAAAAGGTTG+AGG - chr5.1:76282215-76282234 MS.gene37934:CDS 40.0%
TAAGATTCCTAGCCACACTA+TGG - chr5.1:76282986-76283005 MS.gene37934:CDS 40.0%
TCACACCAAGTTCCAGATAT+GGG - chr5.1:76282163-76282182 MS.gene37934:CDS 40.0%
TTATGTGTGGATGAAGATGC+AGG - chr5.1:76282245-76282264 MS.gene37934:CDS 40.0%
TTCACACCAAGTTCCAGATA+TGG - chr5.1:76282162-76282181 MS.gene37934:CDS 40.0%
TTCCCTCCAATCAATTTAGC+CGG + chr5.1:76282945-76282964 None:intergenic 40.0%
! AATCGCGTTTCGGAAGTTTT+TGG - chr5.1:76282624-76282643 MS.gene37934:CDS 40.0%
! ATCGCGTTTCGGAAGTTTTT+GGG - chr5.1:76282625-76282644 MS.gene37934:CDS 40.0%
! GTTGGTGAAACTGCTGTTAT+TGG - chr5.1:76282714-76282733 MS.gene37934:CDS 40.0%
! TTGATCCCATATCTGGAACT+TGG + chr5.1:76282171-76282190 None:intergenic 40.0%
!! TTTGGGGTTGATATTCATCC+TGG - chr5.1:76282642-76282661 MS.gene37934:CDS 40.0%
ACATGATCCATAGTGTGGCT+AGG + chr5.1:76282996-76283015 None:intergenic 45.0%
CTGGAGCTAAGATTGGAAGT+GGG - chr5.1:76282661-76282680 MS.gene37934:CDS 45.0%
GGGAATCCGGCTAAATTGAT+TGG - chr5.1:76282936-76282955 MS.gene37934:CDS 45.0%
GTGCAACATGATCCATAGTG+TGG + chr5.1:76283001-76283020 None:intergenic 45.0%
TGATCAAGGAAGTTCCTCCA+AGG - chr5.1:76282901-76282920 MS.gene37934:CDS 45.0%
TGATCATGCAACTGGGATTG+TGG - chr5.1:76282692-76282711 MS.gene37934:CDS 45.0%
TTTAGCCGGATTCCCAACAA+CGG + chr5.1:76282931-76282950 None:intergenic 45.0%
! ATTTTGCACGGTGTGACGTT+GGG - chr5.1:76282750-76282769 MS.gene37934:CDS 45.0%
! GATGGTGCTAAAATTGGTGC+TGG - chr5.1:76282870-76282889 MS.gene37934:CDS 45.0%
! TATTTTGCACGGTGTGACGT+TGG - chr5.1:76282749-76282768 MS.gene37934:CDS 45.0%
! TCGCGTTTCGGAAGTTTTTG+GGG - chr5.1:76282626-76282645 MS.gene37934:CDS 45.0%
!! CAAGGTGTTGATCCCATATC+TGG + chr5.1:76282178-76282197 None:intergenic 45.0%
!! TGCTGGTTCAGTTGTGATCA+AGG - chr5.1:76282887-76282906 MS.gene37934:CDS 45.0%
AGGCACCCAAAGATTGGTGA+CGG - chr5.1:76282798-76282817 MS.gene37934:CDS 50.0%
CATGCAACTGGGATTGTGGT+TGG - chr5.1:76282696-76282715 MS.gene37934:CDS 50.0%
CCACTTCCAATCTTAGCTCC+AGG + chr5.1:76282663-76282682 None:intergenic 50.0%
CCTGGAGCTAAGATTGGAAG+TGG - chr5.1:76282660-76282679 MS.gene37934:CDS 50.0%
CTCCAAGGACTACCGTTGTT+GGG - chr5.1:76282916-76282935 MS.gene37934:CDS 50.0%
GACTACCGTTGTTGGGAATC+CGG - chr5.1:76282923-76282942 MS.gene37934:CDS 50.0%
TTCCCAACAACGGTAGTCCT+TGG + chr5.1:76282921-76282940 None:intergenic 50.0%
! AGGCGCGGGAACTTGTATTT+TGG - chr5.1:76282830-76282849 MS.gene37934:CDS 50.0%
! GCGCGGGAACTTGTATTTTG+GGG - chr5.1:76282832-76282851 MS.gene37934:CDS 50.0%
! GGCGCGGGAACTTGTATTTT+GGG - chr5.1:76282831-76282850 MS.gene37934:CDS 50.0%
!! ATTGGTGACGGGGTGCTTAT+AGG - chr5.1:76282810-76282829 MS.gene37934:CDS 50.0%
AGCACCCCGTCACCAATCTT+TGG + chr5.1:76282807-76282826 None:intergenic 55.0%
CCAACAACGGTAGTCCTTGG+AGG + chr5.1:76282918-76282937 None:intergenic 55.0%
CCTATCGCCATGTGCCTTAC+CGG + chr5.1:76282781-76282800 None:intergenic 55.0%
CCTCCAAGGACTACCGTTGT+TGG - chr5.1:76282915-76282934 MS.gene37934:CDS 55.0%
GCACCCCGTCACCAATCTTT+GGG + chr5.1:76282806-76282825 None:intergenic 55.0%
GGCGATAGGCACCCAAAGAT+TGG - chr5.1:76282792-76282811 MS.gene37934:CDS 55.0%
! GGCACCCAAAGATTGGTGAC+GGG - chr5.1:76282799-76282818 MS.gene37934:CDS 55.0%
!! GCACCCAAAGATTGGTGACG+GGG - chr5.1:76282800-76282819 MS.gene37934:CDS 55.0%
CCGGTAAGGCACATGGCGAT+AGG - chr5.1:76282778-76282797 MS.gene37934:CDS 60.0%
TGCACGGTGTGACGTTGGGA+GGG - chr5.1:76282754-76282773 MS.gene37934:CDS 60.0%
TTGCACGGTGTGACGTTGGG+AGG - chr5.1:76282753-76282772 MS.gene37934:CDS 60.0%
! TGACGGGGTGCTTATAGGCG+CGG - chr5.1:76282815-76282834 MS.gene37934:CDS 60.0%
GACGTTGGGAGGGACCGGTA+AGG - chr5.1:76282764-76282783 MS.gene37934:CDS 65.0%
GGAGGGACCGGTAAGGCACA+TGG - chr5.1:76282771-76282790 MS.gene37934:CDS 65.0%
GGTGTGACGTTGGGAGGGAC+CGG - chr5.1:76282759-76282778 MS.gene37934:CDS 65.0%
! GACGGGGTGCTTATAGGCGC+GGG - chr5.1:76282816-76282835 MS.gene37934:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 76282087 76283052 76282087 ID=MS.gene37934
chr5.1 mRNA 76282087 76283052 76282087 ID=MS.gene37934.t1;Parent=MS.gene37934
chr5.1 exon 76282087 76283052 76282087 ID=MS.gene37934.t1.exon1;Parent=MS.gene37934.t1
chr5.1 CDS 76282087 76283052 76282087 ID=cds.MS.gene37934.t1;Parent=MS.gene37934.t1
Gene Sequence

>MS.gene37934

ATGTCTGTTTCTTCTCCTGTTGATGCTAATTCAAAGAATCTTTTACCACATGATGATCATTCATGTGAAAAATTCTTCACACCAAGTTCCAGATATGGGATCAACACCTTGCATACAAGTTCAGTTGTGGAACAATCTGAAAAGGTTGAGGATGAAGATTATGTGTGGATGAAGATGCAGGAAGAAGCTAAAGTTGATGTCACTGCTGAACCAATTTTGTCTAGTTACTATTATGTTTCAATCTTGTCACAGAAATCATTGGAAACTGCTTTGGCTAATCACCTTGCTGTTAAATTAAGCAGTGTAAGTCTTCCTAGTACAACACTTTCTGATCTTTTTGTTAGAATTTTGGAATCTGATAAAGAAATTATGTATGCTATGAAAAATGATCTAAAAGCAGTTAATGAAAGAGACCCTGCTTGTATAAGTCATGTTCATTGTTTCTTGAATTTCAAAGGCTTTTTAGCATGTCAAGCTCATAGGGTAGCTCATAATTTGTGGTCAAATGGAAGGAAAATTTTAGCAGTTATGATTCAGAATCGCGTTTCGGAAGTTTTTGGGGTTGATATTCATCCTGGAGCTAAGATTGGAAGTGGGATTTTGCTTGATCATGCAACTGGGATTGTGGTTGGTGAAACTGCTGTTATTGGAAATGATGTGTCTATTTTGCACGGTGTGACGTTGGGAGGGACCGGTAAGGCACATGGCGATAGGCACCCAAAGATTGGTGACGGGGTGCTTATAGGCGCGGGAACTTGTATTTTGGGGAATATTAAGATTGGTGATGGTGCTAAAATTGGTGCTGGTTCAGTTGTGATCAAGGAAGTTCCTCCAAGGACTACCGTTGTTGGGAATCCGGCTAAATTGATTGGAGGGAAGAATAATCCTATTAAACTTGATAAGATTCCTAGCCACACTATGGATCATGTTGCACATATTTCCGAGTTTTATGATTATGTTATCTAG

Protein sequence

>MS.gene37934.t1

MSVSSPVDANSKNLLPHDDHSCEKFFTPSSRYGINTLHTSSVVEQSEKVEDEDYVWMKMQEEAKVDVTAEPILSSYYYVSILSQKSLETALANHLAVKLSSVSLPSTTLSDLFVRILESDKEIMYAMKNDLKAVNERDPACISHVHCFLNFKGFLACQAHRVAHNLWSNGRKILAVMIQNRVSEVFGVDIHPGAKIGSGILLDHATGIVVGETAVIGNDVSILHGVTLGGTGKAHGDRHPKIGDGVLIGAGTCILGNIKIGDGAKIGAGSVVIKEVPPRTTVVGNPAKLIGGKNNPIKLDKIPSHTMDHVAHISEFYDYVI