AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38288

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38288.t1 MTR_4g009040 98.462 325 5 0 1 325 1 325 0.0 659
MS.gene38288.t1 MTR_4g009040 97.264 329 5 1 1 325 1 329 0.0 654
MS.gene38288.t1 MTR_4g009690 93.865 326 20 0 1 326 1 326 0.0 613
MS.gene38288.t1 MTR_4g009690 93.558 326 20 1 1 326 1 325 0.0 607
MS.gene38288.t1 MTR_4g006940 46.914 324 160 4 4 323 11 326 9.78e-92 277
MS.gene38288.t1 MTR_2g104960 47.619 315 156 3 13 323 20 329 1.72e-89 271
MS.gene38288.t1 MTR_4g006940 49.819 277 131 3 4 280 11 279 1.67e-84 256
MS.gene38288.t1 MTR_4g006940 49.819 277 131 3 4 280 11 279 1.67e-84 256
MS.gene38288.t1 MTR_5g072620 41.250 320 180 3 10 323 2 319 3.65e-82 252
MS.gene38288.t1 MTR_2g101330 41.195 318 183 4 12 325 4 321 4.74e-78 241
MS.gene38288.t1 MTR_3g005170 42.675 314 170 7 13 319 10 320 1.14e-77 241
MS.gene38288.t1 MTR_3g005210 42.453 318 173 7 9 319 2 316 3.42e-77 239
MS.gene38288.t1 MTR_4g077100 41.640 317 173 8 11 319 9 321 5.71e-74 231
MS.gene38288.t1 MTR_3g031650 39.308 318 189 2 10 323 2 319 9.58e-74 230
MS.gene38288.t1 MTR_8g062440 42.056 321 173 9 11 323 6 321 1.45e-73 230
MS.gene38288.t1 MTR_8g062440 42.236 322 172 10 11 323 6 322 9.16e-73 228
MS.gene38288.t1 MTR_4g077130 40.938 320 177 8 8 319 4 319 6.53e-72 226
MS.gene38288.t1 MTR_3g005170 43.911 271 145 6 13 279 10 277 6.78e-71 221
MS.gene38288.t1 MTR_8g062110 41.304 322 176 8 10 323 6 322 1.47e-70 222
MS.gene38288.t1 MTR_8g062110 41.824 318 172 8 10 319 15 327 1.52e-70 223
MS.gene38288.t1 MTR_2g028620 40.061 327 182 6 8 324 5 327 2.53e-70 222
MS.gene38288.t1 MTR_4g077100 42.857 273 149 6 11 279 9 278 6.87e-68 214
MS.gene38288.t1 MTR_5g056600 36.478 318 191 5 14 323 5 319 6.45e-67 213
MS.gene38288.t1 MTR_3g031650 39.925 268 160 1 10 276 2 269 1.04e-66 211
MS.gene38288.t1 MTR_8g062440 44.030 268 142 7 11 274 6 269 5.50e-66 211
MS.gene38288.t1 MTR_3g005170 38.854 314 151 7 13 319 10 289 3.75e-65 207
MS.gene38288.t1 MTR_2g028620 43.431 274 148 4 8 276 5 276 4.95e-65 208
MS.gene38288.t1 MTR_1g022440 37.267 322 187 11 13 323 8 325 6.63e-65 208
MS.gene38288.t1 MTR_1g022445 36.957 322 188 10 13 323 8 325 1.11e-63 205
MS.gene38288.t1 MTR_8g062110 40.972 288 155 7 10 293 6 282 1.12e-63 203
MS.gene38288.t1 MTR_8g062110 42.279 272 149 6 10 277 6 273 1.27e-63 203
MS.gene38288.t1 MTR_4g092080 38.554 332 178 11 11 322 12 337 1.69e-57 189
MS.gene38288.t1 MTR_7g074820 35.976 328 189 10 12 323 7 329 3.83e-51 172
MS.gene38288.t1 MTR_7g074880 34.056 323 199 7 12 323 7 326 5.43e-51 172
MS.gene38288.t1 MTR_4g081440 34.188 351 198 11 1 325 8 351 8.92e-51 172
MS.gene38288.t1 MTR_4g081440 33.811 349 203 10 1 325 12 356 9.09e-50 169
MS.gene38288.t1 MTR_7g074730 34.154 325 198 9 12 323 7 328 3.49e-49 167
MS.gene38288.t1 MTR_7g074850 33.129 326 201 9 12 323 7 329 2.70e-46 160
MS.gene38288.t1 MTR_4g023730 36.418 335 186 12 8 323 3 329 4.88e-45 156
MS.gene38288.t1 MTR_7g074710 33.538 325 198 12 12 323 7 326 8.43e-43 150
MS.gene38288.t1 MTR_8g062710 75.532 94 13 1 12 105 19 102 5.87e-41 139
MS.gene38288.t1 MTR_0164s0070 52.756 127 58 2 12 136 4 130 4.34e-36 129
MS.gene38288.t1 MTR_8g062110 35.217 230 138 6 101 323 11 236 7.26e-36 130
MS.gene38288.t1 MTR_5g029990 32.143 308 169 10 13 303 12 296 7.79e-36 131
MS.gene38288.t1 MTR_7g074870 32.384 281 178 7 52 323 11 288 2.10e-34 127
MS.gene38288.t1 MTR_8g062708 85.938 64 9 0 218 281 5 68 4.64e-31 113
MS.gene38288.t1 MTR_6g406250 31.559 263 171 4 13 269 55 314 7.02e-31 119
MS.gene38288.t1 MTR_8g062110 35.000 180 111 4 101 277 11 187 3.56e-29 111
MS.gene38288.t1 MTR_2g013230 28.902 346 200 14 12 323 7 340 1.97e-26 107
MS.gene38288.t1 MTR_7g117190 28.746 327 203 12 12 312 6 328 1.00e-25 105
MS.gene38288.t1 MTR_7g117190 30.000 290 174 11 12 276 6 291 2.09e-25 103
MS.gene38288.t1 MTR_6g406250 31.068 206 138 2 13 214 55 260 4.33e-25 102
MS.gene38288.t1 MTR_2g013250 28.190 337 210 12 12 321 6 337 7.87e-25 102
MS.gene38288.t1 MTR_0685s0030 33.125 160 99 3 165 319 2 158 3.49e-21 89.0
MS.gene38288.t1 MTR_2g013260 27.596 337 212 12 12 321 7 338 1.64e-19 88.2
MS.gene38288.t1 MTR_2g013260 37.748 151 81 7 12 154 7 152 2.09e-18 84.3
MS.gene38288.t1 MTR_3g011130 35.897 117 70 4 104 216 30 145 8.46e-16 73.9
MS.gene38288.t1 MTR_0685s0030 32.174 115 77 1 165 279 2 115 1.50e-15 72.4
MS.gene38288.t1 MTR_3g101500 29.644 253 152 11 11 257 2 234 1.36e-12 67.8
MS.gene38288.t1 MTR_3g101500 29.644 253 152 11 11 257 2 234 1.93e-12 67.4
MS.gene38288.t1 MTR_3g101500 32.292 192 107 10 11 199 2 173 4.60e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38288.t1 AT2G33590 65.176 313 108 1 12 323 8 320 1.20e-146 416
MS.gene38288.t1 AT2G33600 63.259 313 114 1 12 323 8 320 2.23e-135 387
MS.gene38288.t1 AT1G76470 55.975 318 135 2 11 323 5 322 6.04e-115 335
MS.gene38288.t1 AT1G80820 44.828 319 167 3 9 323 4 317 6.84e-86 262
MS.gene38288.t1 AT1G51410 45.031 322 165 8 11 324 7 324 1.01e-83 256
MS.gene38288.t1 AT1G15950 44.969 318 166 3 10 323 10 322 8.58e-83 254
MS.gene38288.t1 AT1G15950 44.969 318 166 3 10 323 10 322 8.58e-83 254
MS.gene38288.t1 AT1G09510 45.511 323 166 7 8 323 3 322 1.10e-81 251
MS.gene38288.t1 AT5G19440 43.505 331 174 8 1 324 1 325 7.14e-81 249
MS.gene38288.t1 AT1G15950 44.805 308 161 3 10 313 10 312 2.07e-79 245
MS.gene38288.t1 AT1G15950 48.188 276 138 2 10 285 10 280 6.60e-79 242
MS.gene38288.t1 AT1G15950 48.718 273 135 2 10 282 10 277 7.59e-79 243
MS.gene38288.t1 AT1G80820 48.162 272 136 2 9 280 4 270 1.03e-78 241
MS.gene38288.t1 AT1G15950 48.708 271 134 2 10 280 10 275 9.80e-78 239
MS.gene38288.t1 AT5G58490 41.587 315 180 2 13 323 9 323 7.37e-77 238
MS.gene38288.t1 AT1G09490 42.006 319 177 6 11 323 6 322 4.57e-75 234
MS.gene38288.t1 AT4G35420 40.432 324 176 7 12 325 7 323 4.34e-74 231
MS.gene38288.t1 AT2G02400 38.486 317 185 5 13 323 6 318 6.74e-74 231
MS.gene38288.t1 AT1G09480 42.947 319 174 6 11 323 6 322 9.65e-74 230
MS.gene38288.t1 AT1G09480 42.947 319 174 6 11 323 53 369 2.35e-73 231
MS.gene38288.t1 AT1G66800 43.750 320 166 8 11 323 6 318 3.92e-72 226
MS.gene38288.t1 AT1G09500 40.123 324 185 6 8 323 3 325 1.00e-69 220
MS.gene38288.t1 AT1G09500 42.804 271 151 4 8 275 3 272 6.84e-67 211
MS.gene38288.t1 AT5G42800 39.634 328 183 11 7 323 2 325 1.26e-65 211
MS.gene38288.t1 AT1G09490 39.502 281 163 5 48 323 13 291 4.36e-58 189
MS.gene38288.t1 AT1G25460 33.968 315 201 4 15 323 6 319 6.59e-54 179
MS.gene38288.t1 AT1G68540 33.861 316 202 4 15 323 6 321 6.80e-54 179
MS.gene38288.t1 AT1G09500 38.163 283 167 5 48 323 10 291 9.36e-53 175
MS.gene38288.t1 AT2G45400 35.119 336 182 11 13 323 40 364 3.73e-49 168
MS.gene38288.t1 AT4G27250 33.735 332 192 10 1 312 1 324 1.88e-47 163
MS.gene38288.t1 AT1G61720 34.835 333 199 11 10 326 10 340 3.20e-47 162
MS.gene38288.t1 AT4G27250 32.653 343 191 10 1 312 1 334 2.07e-46 160
MS.gene38288.t1 AT1G15950 40.625 224 129 1 104 323 1 224 1.48e-45 155
MS.gene38288.t1 AT2G45400 35.540 287 157 8 13 280 40 317 2.93e-43 152
MS.gene38288.t1 AT2G23910 32.609 276 168 6 14 282 11 275 4.02e-43 150
MS.gene38288.t1 AT2G23910 33.210 271 160 6 14 274 11 270 4.60e-43 150
MS.gene38288.t1 AT2G23910 33.210 271 160 6 14 274 11 270 8.37e-43 150
MS.gene38288.t1 AT4G30470 34.074 270 158 6 14 274 11 269 1.13e-40 144
MS.gene38288.t1 AT2G45400 32.500 320 180 12 27 323 1 307 1.62e-37 136
MS.gene38288.t1 AT2G45400 40.092 217 110 7 13 213 40 252 5.97e-35 128
MS.gene38288.t1 AT2G23910 32.479 234 138 5 50 274 14 236 1.18e-32 122
MS.gene38288.t1 AT1G68540 32.367 207 136 2 121 323 8 214 2.04e-31 117
MS.gene38288.t1 AT5G14700 33.577 274 163 8 9 269 51 318 3.77e-30 118
MS.gene38288.t1 AT5G14700 32.870 216 133 5 9 214 51 264 2.13e-24 100

Find 65 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGTCCTACTCTTGTTT+TGG 0.177937 4.3:-86684601 MS.gene38288:CDS
TCATCTATTGCTTTATCTTT+TGG 0.240227 4.3:+86684876 None:intergenic
CTCAGAGAAACTGCAAAGTT+TGG 0.267800 4.3:-86683429 MS.gene38288:CDS
TTCAGCAAGTGTTCATATTT+CGG 0.273006 4.3:+86685318 None:intergenic
CGTAGTTGATGCAATACTTT+TGG 0.281567 4.3:-86684102 MS.gene38288:CDS
AATAAGAAGGTGTGTGTAAC+TGG 0.296410 4.3:-86686308 MS.gene38288:CDS
TTCAATTTCGCGAAAAGAAC+TGG 0.300306 4.3:-86684639 MS.gene38288:CDS
ATATGAACACTTGCTGAAAC+TGG 0.306288 4.3:-86685313 MS.gene38288:CDS
CACTATCAACACACGAGATT+TGG 0.344680 4.3:-86684033 MS.gene38288:CDS
AAAGTTTGGGTTGGAAATTC+AGG 0.345192 4.3:-86683415 MS.gene38288:CDS
ACTCTTCAAAGCAGATATCT+TGG 0.353367 4.3:-86685268 MS.gene38288:CDS
CTTCGTAGGGTACTTGTAGT+TGG 0.358423 4.3:+86683986 None:intergenic
TTTGTGGTTGACTGTAAAAT+TGG 0.363006 4.3:+86684576 None:intergenic
CTGTTCTTCTGCTTCTGTCT+TGG 0.363217 4.3:+86684665 None:intergenic
CTTAGAAGGCCATGACTCAT+TGG 0.391769 4.3:-86684159 MS.gene38288:intron
TCAGAGAAACTGCAAAGTTT+GGG 0.410030 4.3:-86683428 MS.gene38288:CDS
TCCATGGACAAAGTAACCTT+TGG 0.412376 4.3:+86686241 None:intergenic
CATTGGAGAATAAGCTTCGT+TGG 0.422203 4.3:-86684142 MS.gene38288:CDS
TGACTCTGTTGAGAGCTATA+AGG 0.425631 4.3:-86683372 MS.gene38288:CDS
TTCGTAGGGTACTTGTAGTT+GGG 0.439609 4.3:+86683987 None:intergenic
AAGCAATAGATGAATCTTGT+TGG 0.440763 4.3:-86684866 MS.gene38288:CDS
CAAGACAGAAGCAGAAGAAC+AGG 0.442466 4.3:-86684664 MS.gene38288:CDS
AGCCAAGCTACTTGAATTTG+TGG 0.447809 4.3:+86684560 None:intergenic
TCAGTTTACTCCGCAATTGT+TGG 0.452722 4.3:-86685237 MS.gene38288:CDS
TATCTGCTTTGAAGAGTGTA+AGG 0.460836 4.3:+86685274 None:intergenic
GAAACTGAAGCTTACTTCGT+AGG 0.466736 4.3:+86683972 None:intergenic
CATGGAACTGTTAGAGAGCC+TGG 0.466759 4.3:-86686224 MS.gene38288:intron
TTAGCAGTTCCTTTCACTGC+AGG 0.467161 4.3:+86684984 None:intergenic
TCCAAAGGTTACTTTGTCCA+TGG 0.474794 4.3:-86686242 MS.gene38288:CDS
GGTACAACTGTTGAAGGTAC+AGG 0.487565 4.3:+86685186 None:intergenic
CAAAACAAGAGTAGGACAAA+TGG 0.495861 4.3:+86684602 None:intergenic
AGAAACGTTACCTCAGGGTT+AGG 0.503091 4.3:+86685165 None:intergenic
AAGGTGTGTGTAACTGGAGC+AGG 0.505575 4.3:-86686302 MS.gene38288:CDS
CAGGAGGGTTTGTAGCATCA+TGG 0.506329 4.3:-86686283 MS.gene38288:CDS
GGGTTAGGTACAACTGTTGA+AGG 0.511645 4.3:+86685180 None:intergenic
AACCACAAATTCAAGTAGCT+TGG 0.514881 4.3:-86684562 MS.gene38288:CDS
AGAAACTGCAAAGTTTGGGT+TGG 0.515747 4.3:-86683424 MS.gene38288:CDS
TATCAACACACGAGATTTGG+TGG 0.518864 4.3:-86684030 MS.gene38288:CDS
GGGTTGGAAATTCAGGCCAT+TGG 0.519755 4.3:-86683408 MS.gene38288:CDS
GTTAAGCTTCTTCTTTCCAA+AGG 0.521805 4.3:-86686257 MS.gene38288:CDS
GTTGAGAGCTATAAGGAGGC+TGG 0.522498 4.3:-86683365 MS.gene38288:CDS
TTGGAAATTCAGGCCATTGG+AGG 0.526899 4.3:-86683405 MS.gene38288:CDS
AAACTGAAGCTTACTTCGTA+GGG 0.530500 4.3:+86683973 None:intergenic
AGGAGATAATGGAAACAAGT+GGG 0.540632 4.3:-86686343 None:intergenic
TCAATGAGTGTTTCCTCCAA+TGG 0.543548 4.3:+86683392 None:intergenic
GTGATTGAGCCTGCAGTGAA+AGG 0.544137 4.3:-86684993 MS.gene38288:CDS
CAGGCTCTCTAACAGTTCCA+TGG 0.553939 4.3:+86686225 None:intergenic
ATGAGAATCACAAGGCAGAC+GGG 0.568344 4.3:-86684076 MS.gene38288:CDS
TGCTGGCAGCTATATTGAGA+TGG 0.571107 4.3:-86683471 MS.gene38288:intron
GGAGATAATGGAAACAAGTG+GGG 0.572345 4.3:-86686342 None:intergenic
ATTGGTCCCAAAACAAGAGT+AGG 0.572490 4.3:+86684594 None:intergenic
GTGTGTGTAACTGGAGCAGG+AGG 0.579894 4.3:-86686299 MS.gene38288:CDS
TATGAGAATCACAAGGCAGA+CGG 0.581333 4.3:-86684077 MS.gene38288:CDS
GAGGAGATAATGGAAACAAG+TGG 0.583352 4.3:-86686344 None:intergenic
AAGGTACAGGGCTAGCAACA+TGG 0.584108 4.3:+86685199 None:intergenic
GGGTGTTAATAACAATAAGA+AGG 0.597736 4.3:-86686321 MS.gene38288:CDS
GCTTATTCTCCAATGAGTCA+TGG 0.616079 4.3:+86684150 None:intergenic
TGCACTGCATCCAACAATTG+CGG 0.621267 4.3:+86685227 None:intergenic
GTACAACTGTTGAAGGTACA+GGG 0.621515 4.3:+86685187 None:intergenic
CTCTGTTGAGAGCTATAAGG+AGG 0.642841 4.3:-86683369 MS.gene38288:CDS
GAGATAATGGAAACAAGTGG+GGG 0.650626 4.3:-86686341 None:intergenic
GAAAAGAACTGGACTTAACG+TGG 0.670351 4.3:-86684628 MS.gene38288:CDS
TTTGGCTTATGAGAATCACA+AGG 0.712000 4.3:-86684084 MS.gene38288:CDS
AACAGTTGTACCTAACCCTG+AGG 0.743171 4.3:-86685175 MS.gene38288:intron
TGTGTGTAACTGGAGCAGGA+GGG 0.744318 4.3:-86686298 MS.gene38288:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAATTGAAAATTAAC+AGG + chr4.3:86683505-86683524 None:intergenic 10.0%
!! TGAAATAAGTTAAATTATAA+TGG + chr4.3:86685443-86685462 None:intergenic 10.0%
!!! TTTTTAATAAGAATTTGATA+AGG + chr4.3:86685181-86685200 None:intergenic 10.0%
!! AAGAATTTCATTACTATATA+TGG - chr4.3:86683621-86683640 MS.gene38288:intron 15.0%
!! AAGTCATTAATTAATTAATG+AGG - chr4.3:86685842-86685861 MS.gene38288:intron 15.0%
!! ACTATAACATAAGATTATAT+AGG + chr4.3:86686184-86686203 None:intergenic 15.0%
!!! ATAACATATTTTGAAACTTA+AGG + chr4.3:86684919-86684938 None:intergenic 15.0%
!! ACATCTCAAATATATATACA+GGG + chr4.3:86683982-86684001 None:intergenic 20.0%
!! AGACAAAGGAAAAAAAAAAT+AGG - chr4.3:86684028-86684047 MS.gene38288:CDS 20.0%
!! ATAAGTTAAATTATAATGGC+AGG + chr4.3:86685439-86685458 None:intergenic 20.0%
!! CCAATGATAATTTGATAAAA+TGG + chr4.3:86686106-86686125 None:intergenic 20.0%
!! GACAAAGGAAAAAAAAAATA+GGG - chr4.3:86684029-86684048 MS.gene38288:CDS 20.0%
!! GGGATTAAATATATATCTAT+CGG + chr4.3:86683962-86683981 None:intergenic 20.0%
!! TATCATTATCATGTAAAACA+AGG + chr4.3:86685241-86685260 None:intergenic 20.0%
!! TCAAAAGAATAAGAAAATAG+AGG + chr4.3:86684288-86684307 None:intergenic 20.0%
!!! CCATTTTATCAAATTATCAT+TGG - chr4.3:86686103-86686122 MS.gene38288:intron 20.0%
!!! CTTTATCTTTTGGTAAATTA+GGG + chr4.3:86684798-86684817 None:intergenic 20.0%
!!! TATGTATCCATAAATTTGTT+TGG + chr4.3:86683706-86683725 None:intergenic 20.0%
!!! TCATTTAAACAATTAGCTTT+TGG + chr4.3:86683783-86683802 None:intergenic 20.0%
!!! TCTATTTTCTTATTCTTTTG+AGG - chr4.3:86684287-86684306 MS.gene38288:intron 20.0%
!!! TCTTATGTTTTTGACATTAA+CGG + chr4.3:86683931-86683950 None:intergenic 20.0%
! AAGAGTCATTTACTACAATT+TGG + chr4.3:86684109-86684128 None:intergenic 25.0%
! ACTATATATGGAAAGAAACA+AGG - chr4.3:86683633-86683652 MS.gene38288:intron 25.0%
! AGAATGTCCAAACAAATTTA+TGG - chr4.3:86683696-86683715 MS.gene38288:intron 25.0%
! AGAGTCATTTACTACAATTT+GGG + chr4.3:86684108-86684127 None:intergenic 25.0%
! AGGAATTTATAGAAGTTATG+TGG + chr4.3:86685265-86685284 None:intergenic 25.0%
! CACATCTCAAATATATATAC+AGG + chr4.3:86683983-86684002 None:intergenic 25.0%
!! ATTTACTACAATTTGGGATT+TGG + chr4.3:86684102-86684121 None:intergenic 25.0%
!! GCTCTCATCAAAATTTTAAA+AGG - chr4.3:86685141-86685160 MS.gene38288:intron 25.0%
!! GCTTTATCTTTTGGTAAATT+AGG + chr4.3:86684799-86684818 None:intergenic 25.0%
!! TCATCTATTGCTTTATCTTT+TGG + chr4.3:86684808-86684827 None:intergenic 25.0%
!! TTAATGAGGAGTATATGTAA+AGG - chr4.3:86685856-86685875 MS.gene38288:intron 25.0%
!!! ATTTTAGTCCCTAAAATTGA+AGG + chr4.3:86683749-86683768 None:intergenic 25.0%
!!! TTCAATTTTAGGGACTAAAA+TGG - chr4.3:86683748-86683767 MS.gene38288:intron 25.0%
!!! TTTTAGCCTTTATAAGTGTA+TGG - chr4.3:86685328-86685347 MS.gene38288:intron 25.0%
AACATGAACAATTTCGATGA+AGG + chr4.3:86684597-86684616 None:intergenic 30.0%
AAGCAATAGATGAATCTTGT+TGG - chr4.3:86684815-86684834 MS.gene38288:intron 30.0%
AATTGAGCTTTACATGACAA+TGG + chr4.3:86684133-86684152 None:intergenic 30.0%
AGTCCTATGATGGTAATAAA+TGG + chr4.3:86683863-86683882 None:intergenic 30.0%
ATACTCTATACAACTTGTGA+TGG - chr4.3:86683723-86683742 MS.gene38288:intron 30.0%
CATCCATTTATTACCATCAT+AGG - chr4.3:86683857-86683876 MS.gene38288:intron 30.0%
GAGATATCAATAACTGTATC+AGG - chr4.3:86685905-86685924 MS.gene38288:intron 30.0%
GGGTGTTAATAACAATAAGA+AGG - chr4.3:86683360-86683379 MS.gene38288:CDS 30.0%
TATAACATGTTGTCTGCTTA+AGG + chr4.3:86684168-86684187 None:intergenic 30.0%
TTCAGCAAGTGTTCATATTT+CGG + chr4.3:86684366-86684385 None:intergenic 30.0%
TTCATCTTGTCTTTCTTAGA+AGG - chr4.3:86685508-86685527 MS.gene38288:intron 30.0%
TTTGTGGTTGACTGTAAAAT+TGG + chr4.3:86685108-86685127 None:intergenic 30.0%
! TATGTTATAGTTTTCCTTGC+TGG - chr4.3:86686193-86686212 MS.gene38288:intron 30.0%
!! TTTGTATACAAGTGTTGAAG+AGG - chr4.3:86684251-86684270 MS.gene38288:intron 30.0%
!!! ATTTGTCCTACTCTTGTTTT+GGG - chr4.3:86685081-86685100 MS.gene38288:intron 30.0%
AAACTGAAGCTTACTTCGTA+GGG + chr4.3:86685711-86685730 None:intergenic 35.0%
AACCACAAATTCAAGTAGCT+TGG - chr4.3:86685119-86685138 MS.gene38288:intron 35.0%
ACTCTTCAAAGCAGATATCT+TGG - chr4.3:86684413-86684432 MS.gene38288:intron 35.0%
AGACTACTGCAAAAATACTC+AGG - chr4.3:86684846-86684865 MS.gene38288:CDS 35.0%
ATGAAACACTGACACATTCA+CGG + chr4.3:86686152-86686171 None:intergenic 35.0%
ATGAGATGAGAATTAGTACC+AGG + chr4.3:86683478-86683497 None:intergenic 35.0%
ATGGAAAGAAACAAGGCTTA+AGG - chr4.3:86683640-86683659 MS.gene38288:intron 35.0%
ATTCCTTGATCAGTTAACAG+CGG - chr4.3:86685279-86685298 MS.gene38288:CDS 35.0%
CAAAACAAGAGTAGGACAAA+TGG + chr4.3:86685082-86685101 None:intergenic 35.0%
GTTAAGCTTCTTCTTTCCAA+AGG - chr4.3:86683424-86683443 MS.gene38288:CDS 35.0%
TAATTTGATATGTCACTGCG+TGG - chr4.3:86684560-86684579 MS.gene38288:CDS 35.0%
TCAGAGAAACTGCAAAGTTT+GGG - chr4.3:86686253-86686272 MS.gene38288:CDS 35.0%
TTCAATTTCGCGAAAAGAAC+TGG - chr4.3:86685042-86685061 MS.gene38288:intron 35.0%
! AATAAGAAGGTGTGTGTAAC+TGG - chr4.3:86683373-86683392 MS.gene38288:CDS 35.0%
! AGTTCTTTTCGCGAAATTGA+AGG + chr4.3:86685043-86685062 None:intergenic 35.0%
! ATATGAACACTTGCTGAAAC+TGG - chr4.3:86684368-86684387 MS.gene38288:intron 35.0%
! TGTGATGGCCTTCAATTTTA+GGG - chr4.3:86683738-86683757 MS.gene38288:intron 35.0%
! TTGTGATGGCCTTCAATTTT+AGG - chr4.3:86683737-86683756 MS.gene38288:intron 35.0%
! TTTGGCTTATGAGAATCACA+AGG - chr4.3:86685597-86685616 MS.gene38288:intron 35.0%
!! AAAGTTTGGGTTGGAAATTC+AGG - chr4.3:86686266-86686285 MS.gene38288:CDS 35.0%
!! CATTTGTCCTACTCTTGTTT+TGG - chr4.3:86685080-86685099 MS.gene38288:intron 35.0%
!! CGTAGTTGATGCAATACTTT+TGG - chr4.3:86685579-86685598 MS.gene38288:intron 35.0%
!! TATCTGCTTTGAAGAGTGTA+AGG + chr4.3:86684410-86684429 None:intergenic 35.0%
AAAACACGATTGCAGTGAAG+CGG + chr4.3:86685372-86685391 None:intergenic 40.0%
AACATGTTGTCTGCTTAAGG+TGG + chr4.3:86684165-86684184 None:intergenic 40.0%
AACGTTAGAAACGTTACCTC+AGG + chr4.3:86684525-86684544 None:intergenic 40.0%
ACGTTAGAAACGTTACCTCA+GGG + chr4.3:86684524-86684543 None:intergenic 40.0%
AGAAACTGCAAAGTTTGGGT+TGG - chr4.3:86686257-86686276 MS.gene38288:CDS 40.0%
AGCCAAGCTACTTGAATTTG+TGG + chr4.3:86685124-86685143 None:intergenic 40.0%
ATTGGTCCCAAAACAAGAGT+AGG + chr4.3:86685090-86685109 None:intergenic 40.0%
ATTGTCGAGTCATGAGAATG+AGG - chr4.3:86685931-86685950 MS.gene38288:intron 40.0%
CACTATCAACACACGAGATT+TGG - chr4.3:86685648-86685667 MS.gene38288:intron 40.0%
CATTGGAGAATAAGCTTCGT+TGG - chr4.3:86685539-86685558 MS.gene38288:intron 40.0%
CTCAGAGAAACTGCAAAGTT+TGG - chr4.3:86686252-86686271 MS.gene38288:CDS 40.0%
GAAAAGAACTGGACTTAACG+TGG - chr4.3:86685053-86685072 MS.gene38288:intron 40.0%
GAAACTGAAGCTTACTTCGT+AGG + chr4.3:86685712-86685731 None:intergenic 40.0%
GACACATGTTAGACACTTCT+TGG + chr4.3:86684007-86684026 None:intergenic 40.0%
GCATGGCCATACACTTATAA+AGG + chr4.3:86685337-86685356 None:intergenic 40.0%
GCTTATTCTCCAATGAGTCA+TGG + chr4.3:86685534-86685553 None:intergenic 40.0%
GTTTCGATGCTACAAAGCAA+TGG + chr4.3:86686013-86686032 None:intergenic 40.0%
TATCAACACACGAGATTTGG+TGG - chr4.3:86685651-86685670 MS.gene38288:intron 40.0%
TATGAGAATCACAAGGCAGA+CGG - chr4.3:86685604-86685623 MS.gene38288:intron 40.0%
TCAATGAGTGTTTCCTCCAA+TGG + chr4.3:86686292-86686311 None:intergenic 40.0%
TCAGTTTACTCCGCAATTGT+TGG - chr4.3:86684444-86684463 MS.gene38288:intron 40.0%
TCCATGGACAAAGTAACCTT+TGG + chr4.3:86683443-86683462 None:intergenic 40.0%
TCTAACATGTGTCGAGACAA+AGG - chr4.3:86684014-86684033 MS.gene38288:CDS 40.0%
TGAAACACTGACACATTCAC+GGG + chr4.3:86686151-86686170 None:intergenic 40.0%
TGACTCTGTTGAGAGCTATA+AGG - chr4.3:86686309-86686328 MS.gene38288:CDS 40.0%
! CTCACAAGAACTTGCTTAAC+AGG + chr4.3:86685888-86685907 None:intergenic 40.0%
! CTTCACTGCAATCGTGTTTT+GGG - chr4.3:86685372-86685391 MS.gene38288:intron 40.0%
! CTTTGTAGCATCGAAACTTC+AGG - chr4.3:86686016-86686035 MS.gene38288:intron 40.0%
! GACACTTTTCGTGTGAGTAT+CGG - chr4.3:86684051-86684070 MS.gene38288:CDS 40.0%
! GTACAACTGTTGAAGGTACA+GGG + chr4.3:86684497-86684516 None:intergenic 40.0%
! TCCAAAGGTTACTTTGTCCA+TGG - chr4.3:86683439-86683458 MS.gene38288:CDS 40.0%
! TTAAAAGTGTGTCGTGTGTC+CGG - chr4.3:86686039-86686058 MS.gene38288:intron 40.0%
! TTCGTAGGGTACTTGTAGTT+GGG + chr4.3:86685697-86685716 None:intergenic 40.0%
!!! TGACACTTCAGACTTTTTCG+TGG + chr4.3:86684634-86684653 None:intergenic 40.0%
!!! TGCAATCGTGTTTTGGGATT+CGG - chr4.3:86685378-86685397 MS.gene38288:intron 40.0%
AAACACGATTGCAGTGAAGC+GGG + chr4.3:86685371-86685390 None:intergenic 45.0%
AACAGTTGTACCTAACCCTG+AGG - chr4.3:86684506-86684525 MS.gene38288:intron 45.0%
ACACCGCTGTTAACTGATCA+AGG + chr4.3:86685285-86685304 None:intergenic 45.0%
AGAAACGTTACCTCAGGGTT+AGG + chr4.3:86684519-86684538 None:intergenic 45.0%
ATGAGAATCACAAGGCAGAC+GGG - chr4.3:86685605-86685624 MS.gene38288:intron 45.0%
CAAGACAGAAGCAGAAGAAC+AGG - chr4.3:86685017-86685036 MS.gene38288:intron 45.0%
CTCTGTTGAGAGCTATAAGG+AGG - chr4.3:86686312-86686331 MS.gene38288:CDS 45.0%
CTGTTCTTCTGCTTCTGTCT+TGG + chr4.3:86685019-86685038 None:intergenic 45.0%
CTTAGAAGGCCATGACTCAT+TGG - chr4.3:86685522-86685541 MS.gene38288:intron 45.0%
GGGTTAGGTACAACTGTTGA+AGG + chr4.3:86684504-86684523 None:intergenic 45.0%
GTACCTGAAGCTTAATCTGC+AGG - chr4.3:86684660-86684679 MS.gene38288:CDS 45.0%
GTCTCTATGTTGACACGAGT+CGG + chr4.3:86684078-86684097 None:intergenic 45.0%
GTTGTCTGCTTAAGGTGGTT+TGG + chr4.3:86684160-86684179 None:intergenic 45.0%
TACGTGTCAGAGTCCTATGA+TGG + chr4.3:86683873-86683892 None:intergenic 45.0%
TCTCAATATAGCTGCCAGCA+AGG + chr4.3:86686210-86686229 None:intergenic 45.0%
TGCACTGCATCCAACAATTG+CGG + chr4.3:86684457-86684476 None:intergenic 45.0%
TGCTGGCAGCTATATTGAGA+TGG - chr4.3:86686210-86686229 MS.gene38288:intron 45.0%
TTAGCAGTTCCTTTCACTGC+AGG + chr4.3:86684700-86684719 None:intergenic 45.0%
TTGGAAATTCAGGCCATTGG+AGG - chr4.3:86686276-86686295 MS.gene38288:CDS 45.0%
! CTTCGTAGGGTACTTGTAGT+TGG + chr4.3:86685698-86685717 None:intergenic 45.0%
! GCTTCACTGCAATCGTGTTT+TGG - chr4.3:86685371-86685390 MS.gene38288:intron 45.0%
! GGTACAACTGTTGAAGGTAC+AGG + chr4.3:86684498-86684517 None:intergenic 45.0%
!! ATATTAATATTTAATTAAAC+AGG + chr4.3:86685757-86685776 None:intergenic 5.0%
AAGGTGTGTGTAACTGGAGC+AGG - chr4.3:86683379-86683398 MS.gene38288:CDS 50.0%
AGCGAATGTCTGACAACTGC+AGG - chr4.3:86684336-86684355 MS.gene38288:intron 50.0%
CAGGAGGGTTTGTAGCATCA+TGG - chr4.3:86683398-86683417 MS.gene38288:CDS 50.0%
CAGGCTCTCTAACAGTTCCA+TGG + chr4.3:86683459-86683478 None:intergenic 50.0%
CATGGAACTGTTAGAGAGCC+TGG - chr4.3:86683457-86683476 MS.gene38288:CDS 50.0%
CCACCTGCAGATTAAGCTTC+AGG + chr4.3:86684666-86684685 None:intergenic 50.0%
CCTGAAGCTTAATCTGCAGG+TGG - chr4.3:86684663-86684682 MS.gene38288:CDS 50.0%
CTGCAAGTAATGTTGCGCGA+TGG - chr4.3:86685964-86685983 MS.gene38288:intron 50.0%
GGGTTGGAAATTCAGGCCAT+TGG - chr4.3:86686273-86686292 MS.gene38288:CDS 50.0%
GTGATTGAGCCTGCAGTGAA+AGG - chr4.3:86684688-86684707 MS.gene38288:intron 50.0%
GTTGAGAGCTATAAGGAGGC+TGG - chr4.3:86686316-86686335 MS.gene38288:CDS 50.0%
TGTGTGTAACTGGAGCAGGA+GGG - chr4.3:86683383-86683402 MS.gene38288:CDS 50.0%
! AAGGTACAGGGCTAGCAACA+TGG + chr4.3:86684485-86684504 None:intergenic 50.0%
! AGTGAAGCGGGTAGTGGTAT+GGG + chr4.3:86685359-86685378 None:intergenic 50.0%
! TGTCAGTACCGAGTCAATGC+CGG + chr4.3:86686061-86686080 None:intergenic 50.0%
GATTGCAGTGAAGCGGGTAG+TGG + chr4.3:86685365-86685384 None:intergenic 55.0%
GTGTGTGTAACTGGAGCAGG+AGG - chr4.3:86683382-86683401 MS.gene38288:CDS 55.0%
! CAGTGAAGCGGGTAGTGGTA+TGG + chr4.3:86685360-86685379 None:intergenic 55.0%
!! TCGTGTGTCCGGCATTGACT+CGG - chr4.3:86686050-86686069 MS.gene38288:intron 55.0%
CTTCGCACGTGACGCAGAAC+TGG - chr4.3:86684983-86685002 MS.gene38288:CDS 60.0%
! AGCGGGTAGTGGTATGGGCA+TGG + chr4.3:86685354-86685373 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 86683346 86686357 86683346 ID=MS.gene38288
chr4.3 mRNA 86683346 86686357 86683346 ID=MS.gene38288.t1;Parent=MS.gene38288
chr4.3 exon 86686225 86686357 86686225 ID=MS.gene38288.t1.exon1;Parent=MS.gene38288.t1
chr4.3 CDS 86686225 86686357 86686225 ID=cds.MS.gene38288.t1;Parent=MS.gene38288.t1
chr4.3 exon 86685176 86685345 86685176 ID=MS.gene38288.t1.exon2;Parent=MS.gene38288.t1
chr4.3 CDS 86685176 86685345 86685176 ID=cds.MS.gene38288.t1;Parent=MS.gene38288.t1
chr4.3 exon 86684836 86685021 86684836 ID=MS.gene38288.t1.exon3;Parent=MS.gene38288.t1
chr4.3 CDS 86684836 86685021 86684836 ID=cds.MS.gene38288.t1;Parent=MS.gene38288.t1
chr4.3 exon 86684541 86684703 86684541 ID=MS.gene38288.t1.exon4;Parent=MS.gene38288.t1
chr4.3 CDS 86684541 86684703 86684541 ID=cds.MS.gene38288.t1;Parent=MS.gene38288.t1
chr4.3 exon 86683987 86684176 86683987 ID=MS.gene38288.t1.exon5;Parent=MS.gene38288.t1
chr4.3 CDS 86683987 86684176 86683987 ID=cds.MS.gene38288.t1;Parent=MS.gene38288.t1
chr4.3 exon 86683346 86683484 86683346 ID=MS.gene38288.t1.exon6;Parent=MS.gene38288.t1
chr4.3 CDS 86683346 86683484 86683346 ID=cds.MS.gene38288.t1;Parent=MS.gene38288.t1
Gene Sequence

>MS.gene38288

ATGGAAACAAGTGGGGGTGTTAATAACAATAAGAAGGTGTGTGTAACTGGAGCAGGAGGGTTTGTAGCATCATGGCTTGTTAAGCTTCTTCTTTCCAAAGGTTACTTTGTCCATGGAACTGTTAGAGAGCCTGGTACTAATTCTCATCTCATTATTCCTGTTAATTTTCAATTTTTTTAGAATTAATTATAAATATCTTAGTTGTTATTGAGAAATATTCTAATACATTGTTGTTAATGTTATATATGTATAGTATACGTTTATTTTAGTAAGCAAAGAATTTCATTACTATATATGGAAAGAAACAAGGCTTAAGGACATGTAATTTTGATGCTGTAAGAGAGATTAAGAGAATGTCCAAACAAATTTATGGATACATACTCTATACAACTTGTGATGGCCTTCAATTTTAGGGACTAAAATGGTGATTCAATCCAAAAGCTAATTGTTTAAATGAAAGTCAACTAATACGTAGCAACTTGCAAAACTAGAGCAAATTAACAATATATCTCATCCATTTATTACCATCATAGGACTCTGACACGTACAAGTCAACATTGTTAATGTCAAAAACATAGAACACCGTTAATGTCAAAAACATAAGACACTGACACCGATAGATATATATTTAATCCCTGTATATATATTTGAGATGTGTCCAAGAAGTGTCTAACATGTGTCGAGACAAAGGAAAAAAAAAATAGGGACACTTTTCGTGTGAGTATCGGACCGACTCGTGTCAACATAGAGACGCCAAATCCCAAATTGTAGTAAATGACTCTTGCCATTGTCATGTAAAGCTCAATTCAGACCAAACCACCTTAAGCAGACAACATGTTATACTTGTATATGTAATATAAATTGTTGATTTTTCATTATTAGCTAATACTTTCTTAGTACACTATTTTGTATACAAGTGTTGAAGAGGATGTTGTTATGCCTCTATTTTCTTATTCTTTTGAGGTACGAGTGTTGTTGCGATTCATTTGAAGCGAATGTCTGACAACTGCAGGTAGTCCGAAATATGAACACTTGCTGAAACTGGAAAAAGCTTCTGAGAACCTTACACTCTTCAAAGCAGATATCTTGGATTACGAATCAGTTTACTCCGCAATTGTTGGATGCAGTGCAGTTTTCCATGTTGCTAGCCCTGTACCTTCAACAGTTGTACCTAACCCTGAGGTAACGTTTCTAACGTTCTCTTATATGCAGACTAATTTGATATGTCACTGCGTGGTTCTAGTTTTGCCTTCATCGAAATTGTTCATGTTAGCAAACTCGATATCCACGAAAAAGTCTGAAGTGTCAAATAATGTACCTGAAGCTTAATCTGCAGGTGGAAGTGATTGAGCCTGCAGTGAAAGGAACTGCTAATGTACTTGAAGCTTGTCTCGAAGCTAATGTTGAACGTGTCGTATTTGTATCATCTGCAGCTGCTGTTGCTATCAACCCTAATTTACCAAAAGATAAAGCAATAGATGAATCTTGTTGGTCTGATAAAGACTACTGCAAAAATACTCAGGTCACAAAGATTTTATTATATTTGTACATGACATAAATGTATCATTAGCCTTAAGTTTCAAAATATGTTATGTTTCAATCATCAAAGAATTTTGTGTTATGCAAATAAGCTTATACTTCGCACGTGACGCAGAACTGGTATTGTTATGCCAAGACAGAAGCAGAAGAACAGGCCTTCAATTTCGCGAAAAGAACTGGACTTAACGTGGTAACCATTTGTCCTACTCTTGTTTTGGGACCAATTTTACAGTCAACCACAAATTCAAGTAGCTTGGCTCTCATCAAAATTTTAAAAGGTACTTACTAATATGCCTTATCAAATTCTTATTAAAAAATACATGTTTATGAACTGTACAATGCGAGTTAAAATTCCTTGTTTTACATGATAATGATAACCACATAACTTCTATAAATTCCTTGATCAGTTAACAGCGGTGTTTGATTTTGTGCATTCTTCTATTTTTTAGCCTTTATAAGTGTATGGCCATGCCCATACCACTACCCGCTTCACTGCAATCGTGTTTTGGGATTCGGCTGATATGCTACGCTATCTGCTGCTAACTTGTTTTCCTGCCATTATAATTTAACTTATTTCATACATTATAGATTTCATATAAATATTAAAATTGTTTTTCATGTTATTCATCTTGTCTTTCTTAGAAGGCCATGACTCATTGGAGAATAAGCTTCGTTGGATTGTTGACGTGCGAGACGTAGTTGATGCAATACTTTTGGCTTATGAGAATCACAAGGCAGACGGGAGATACATTTGCACTTCACACACTATCAACACACGAGATTTGGTGGAGAGATTAAAGAGTCTATATCCCAACTACAAGTACCCTACGAAGTAAGCTTCAGTTTCTAAAGTAGTAGTCATAATTCAACCTGTTTAATTAAATATTAATATTATCAAGATATATAAGAAATATGTAAGAAACGTGAAAAGAATTTGATATTAACAGAAAACTAAAGAAGTCATTAATTAATTAATGAGGAGTATATGTAAAGGAGATAACCTGTTAAGCAAGTTCTTGTGAGATATCAATAACTGTATCAGGAAAATTGTCGAGTCATGAGAATGAGGAAGAAAACAACTGCAAGTAATGTTGCGCGATGGAGTACTTGCAGTGTAAATCTTAACCATTGCTTTGTAGCATCGAAACTTCAGGTTAAAAGTGTGTCGTGTGTCCGGCATTGACTCGGTACTGACACATGTTTTTAAATTCAATACTTCCATTTTATCAAATTATCATTGGTGTTAGTGTGTCAGTGCTGTGTCCCGTGAATGTGTCAGTGTTTCATAGCTACTTGCCTATATAATCTTATGTTATAGTTTTCCTTGCTGGCAGCTATATTGAGATGGATGATTACAAAATGCTGAGCTCAGAGAAACTGCAAAGTTTGGGTTGGAAATTCAGGCCATTGGAGGAAACACTCATTGACTCTGTTGAGAGCTATAAGGAGGCTGGACTACTTCAATCAGAATAA
Protein sequence

>MS.gene38288.t1

METSGGVNNNKKVCVTGAGGFVASWLVKLLLSKGYFVHGTVREPGSPKYEHLLKLEKASENLTLFKADILDYESVYSAIVGCSAVFHVASPVPSTVVPNPEVEVIEPAVKGTANVLEACLEANVERVVFVSSAAAVAINPNLPKDKAIDESCWSDKDYCKNTQNWYCYAKTEAEEQAFNFAKRTGLNVVTICPTLVLGPILQSTTNSSSLALIKILKEGHDSLENKLRWIVDVRDVVDAILLAYENHKADGRYICTSHTINTRDLVERLKSLYPNYKYPTNYIEMDDYKMLSSEKLQSLGWKFRPLEETLIDSVESYKEAGLLQSE