AlfalfaGEDB | MS.gene38327

M. sativa cultivar XinJiangDaYe / MS.gene38327

KEGG: -
GO: -
PFAM: PF04755.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38327.t1	XP_013448320.1	84.9	152	15	1	9	160	3	146	3.20E-61	244.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38327.t1	O81304	64.7	133	39	1	28	160	31	155	2.9e-39	162.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38327.t1	A0A072TY66	84.9	152	15	1	9	160	3	146	2.3e-61	244.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050670	MS.gene38327	0.801931	7.13E-49	-1.69E-46
MS.gene052485	MS.gene38327	0.804576	2.02E-49	-1.69E-46
MS.gene057476	MS.gene38327	0.804636	1.96E-49	-1.69E-46
MS.gene057749	MS.gene38327	0.829196	5.89E-55	-1.69E-46
MS.gene057823	MS.gene38327	0.821876	3.20E-53	-1.69E-46
MS.gene057826	MS.gene38327	0.803902	2.79E-49	-1.69E-46
MS.gene058995	MS.gene38327	0.812071	5.10E-51	-1.69E-46
MS.gene059420	MS.gene38327	0.80112	1.05E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38327.t1	MTR_7g444820	84.932	146	14	1	15	160	9	146	6.23e-83	243

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38327.t1	AT4G00030	64.179	134	40	1	27	160	30	155	2.54e-53	168

Find 43 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGCTTGGTGGGGTTGTTT+TGG	0.185547	7.3:-57716473	None:intergenic
GGAAGTGGTGGCGGTGGTGG+TGG	0.278336	7.3:-57716346	None:intergenic
AGTGGTGGCGGTGGTGGTGG+TGG	0.299425	7.3:-57716343	None:intergenic
GTTTGAGAGGTGAATGGGTT+GGG	0.302248	7.3:-57716378	None:intergenic
ATTAAGAAGGCTGGATTGTT+TGG	0.325343	7.3:+57716618	MS.gene38327:CDS
GGTGATTGAGTTGAGTTTCA+TGG	0.349798	7.3:-57716322	None:intergenic
AAGGGAAGTGGTGGCGGTGG+TGG	0.350059	7.3:-57716349	None:intergenic
GGGTCTTGAGGCCACGGTCT+TGG	0.384643	7.3:-57716452	None:intergenic
CGACAACTTGGCGGTTGCTC+TGG	0.385702	7.3:+57716568	MS.gene38327:CDS
GAAGAAAAGGGAAGTGGTGG+CGG	0.400247	7.3:-57716355	None:intergenic
TGAATGGGTTGGGGAAGAAA+AGG	0.412186	7.3:-57716368	None:intergenic
TTGGTGGTTTGAGAGGTGAA+TGG	0.418869	7.3:-57716384	None:intergenic
TTGTTCATCATTAAGAAGGC+TGG	0.434736	7.3:+57716609	MS.gene38327:CDS
GTCGAATATTGAAGTTGCTT+CGG	0.436707	7.3:+57716761	MS.gene38327:CDS
GGATTGTTTGGAACTAAAGC+TGG	0.458772	7.3:+57716630	MS.gene38327:CDS
GGTTTGAGAGGTGAATGGGT+TGG	0.461814	7.3:-57716379	None:intergenic
TGGTGGTTTGAGAGGTGAAT+GGG	0.462983	7.3:-57716383	None:intergenic
GAATGGGTTGGGGAAGAAAA+GGG	0.466912	7.3:-57716367	None:intergenic
GAAAAGGGAAGTGGTGGCGG+TGG	0.470134	7.3:-57716352	None:intergenic
TTTGAGAGGTGAATGGGTTG+GGG	0.476901	7.3:-57716377	None:intergenic
GCAGTTGTTCATCATTAAGA+AGG	0.496553	7.3:+57716605	MS.gene38327:CDS
GGGGAAGAAAAGGGAAGTGG+TGG	0.504240	7.3:-57716358	None:intergenic
CGTTACCGTGATCGATTCAA+TGG	0.505248	7.3:+57716503	MS.gene38327:CDS
ACGATCGACACTAGCTTGGT+GGG	0.505817	7.3:-57716483	None:intergenic
ATCGATTCAATGGTCAAGCT+TGG	0.520360	7.3:+57716513	MS.gene38327:CDS
AACGATCGACACTAGCTTGG+TGG	0.521234	7.3:-57716484	None:intergenic
GTTGGGGAAGAAAAGGGAAG+TGG	0.533661	7.3:-57716361	None:intergenic
GGTAACGATCGACACTAGCT+TGG	0.536165	7.3:-57716487	None:intergenic
CACGGTCTTGGTCAGAGATG+AGG	0.542439	7.3:-57716440	None:intergenic
GTGATTCCCTATCGACAACT+TGG	0.545287	7.3:+57716556	MS.gene38327:CDS
ATTCCCTATCGACAACTTGG+CGG	0.548503	7.3:+57716559	MS.gene38327:CDS
CAACCGCCAAGTTGTCGATA+GGG	0.568619	7.3:-57716562	None:intergenic
ACGGTCTTGGTCAGAGATGA+GGG	0.570760	7.3:-57716439	None:intergenic
GCAACCGCCAAGTTGTCGAT+AGG	0.573035	7.3:-57716563	None:intergenic
TCAATGGTCAAGCTTGGCAT+CGG	0.588669	7.3:+57716519	MS.gene38327:CDS
GGCATCGGTACAGTAACCAC+TGG	0.604053	7.3:+57716534	MS.gene38327:CDS
CTTGGTCAGAGATGAGGGTG+AGG	0.611807	7.3:-57716434	None:intergenic
GTTGCTCTGGACTACTGAGA+AGG	0.629747	7.3:+57716581	MS.gene38327:CDS
GGTCAGAGATGAGGGTGAGG+AGG	0.635921	7.3:-57716431	None:intergenic
CGATCGACACTAGCTTGGTG+GGG	0.639447	7.3:-57716482	None:intergenic
CTTGACCATTGAATCGATCA+CGG	0.654095	7.3:-57716508	None:intergenic
CTCATCTCTGACCAAGACCG+TGG	0.686718	7.3:+57716441	MS.gene38327:CDS
TGTCGATAGGGAATCACCAG+TGG	0.698896	7.3:-57716550	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTAAAAAAAAAAAAACCATC+AGG	-	chr7.3:57716717-57716736	None:intergenic	20.0%
!	AAAAAAAAAAAACCATCAGG+AGG	-	chr7.3:57716714-57716733	None:intergenic	25.0%
!!	AAAATGGTGTTTTCTTTGTT+CGG	+	chr7.3:57716739-57716758	MS.gene38327:CDS	25.0%
	ATTAAGAAGGCTGGATTGTT+TGG	+	chr7.3:57716618-57716637	MS.gene38327:CDS	35.0%
	GCAGTTGTTCATCATTAAGA+AGG	+	chr7.3:57716605-57716624	MS.gene38327:CDS	35.0%
	GTCGAATATTGAAGTTGCTT+CGG	+	chr7.3:57716761-57716780	MS.gene38327:CDS	35.0%
	TTGTTCATCATTAAGAAGGC+TGG	+	chr7.3:57716609-57716628	MS.gene38327:CDS	35.0%
!	TAAAGCTGGAGATGTTTTAC+AGG	+	chr7.3:57716644-57716663	MS.gene38327:CDS	35.0%
!!	GTTATTACTTTTCCTCCTGA+TGG	+	chr7.3:57716699-57716718	MS.gene38327:CDS	35.0%
	CTTGACCATTGAATCGATCA+CGG	-	chr7.3:57716511-57716530	None:intergenic	40.0%
!	ATCGATTCAATGGTCAAGCT+TGG	+	chr7.3:57716513-57716532	MS.gene38327:CDS	40.0%
!	GGATTGTTTGGAACTAAAGC+TGG	+	chr7.3:57716630-57716649	MS.gene38327:CDS	40.0%
!!!	TCTTTTGTTGGTGGTTTGAG+AGG	-	chr7.3:57716394-57716413	None:intergenic	40.0%
!!!	TTTGGCGGTTAGTCTTTTGT+TGG	-	chr7.3:57716406-57716425	None:intergenic	40.0%
	ATTCCCTATCGACAACTTGG+CGG	+	chr7.3:57716559-57716578	MS.gene38327:CDS	45.0%
	GTGATTCCCTATCGACAACT+TGG	+	chr7.3:57716556-57716575	MS.gene38327:CDS	45.0%
	TCAATGGTCAAGCTTGGCAT+CGG	+	chr7.3:57716519-57716538	MS.gene38327:CDS	45.0%
	TGGTGGTTTGAGAGGTGAAT+GGG	-	chr7.3:57716386-57716405	None:intergenic	45.0%
!	CGTTACCGTGATCGATTCAA+TGG	+	chr7.3:57716503-57716522	MS.gene38327:CDS	45.0%
!	GTTTGAGAGGTGAATGGGTT+GGG	-	chr7.3:57716381-57716400	None:intergenic	45.0%
!	TTGGTGGTTTGAGAGGTGAA+TGG	-	chr7.3:57716387-57716406	None:intergenic	45.0%
!!	GAATGGGTTGGGGAAGAAAA+GGG	-	chr7.3:57716370-57716389	None:intergenic	45.0%
!!	TAGCTTGGTGGGGTTGTTTT+GGG	-	chr7.3:57716475-57716494	None:intergenic	45.0%
!!	TGAATGGGTTGGGGAAGAAA+AGG	-	chr7.3:57716371-57716390	None:intergenic	45.0%
!!	TTTGAGAGGTGAATGGGTTG+GGG	-	chr7.3:57716380-57716399	None:intergenic	45.0%
!!!	TTTTTTTTTTTTAGAAAAAA+TGG	+	chr7.3:57716723-57716742	MS.gene38327:CDS	5.0%
	ACGGTCTTGGTCAGAGATGA+GGG	-	chr7.3:57716442-57716461	None:intergenic	50.0%
	CAACCGCCAAGTTGTCGATA+GGG	-	chr7.3:57716565-57716584	None:intergenic	50.0%
	GAAGAAAAGGGAAGTGGTGG+CGG	-	chr7.3:57716358-57716377	None:intergenic	50.0%
	GGTAACGATCGACACTAGCT+TGG	-	chr7.3:57716490-57716509	None:intergenic	50.0%
	GGTTTGAGAGGTGAATGGGT+TGG	-	chr7.3:57716382-57716401	None:intergenic	50.0%
	GTTGGGGAAGAAAAGGGAAG+TGG	-	chr7.3:57716364-57716383	None:intergenic	50.0%
	TGTCGATAGGGAATCACCAG+TGG	-	chr7.3:57716553-57716572	None:intergenic	50.0%
!	AACGATCGACACTAGCTTGG+TGG	-	chr7.3:57716487-57716506	None:intergenic	50.0%
!	ACGATCGACACTAGCTTGGT+GGG	-	chr7.3:57716486-57716505	None:intergenic	50.0%
!	GAGGGTGAGGAGGTTCTTTT+TGG	-	chr7.3:57716424-57716443	None:intergenic	50.0%
!	GGTGAGGAGGTTCTTTTTGG+CGG	-	chr7.3:57716421-57716440	None:intergenic	50.0%
!	GTTGCTCTGGACTACTGAGA+AGG	+	chr7.3:57716581-57716600	MS.gene38327:CDS	50.0%
!!	CTAGCTTGGTGGGGTTGTTT+TGG	-	chr7.3:57716476-57716495	None:intergenic	50.0%
!!	TGTTTTGGGTCTTGAGGCCA+CGG	-	chr7.3:57716461-57716480	None:intergenic	50.0%
!!!	GGCGGTTAGTCTTTTGTTGG+TGG	-	chr7.3:57716403-57716422	None:intergenic	50.0%
!!!	TGGGGTTGTTTTGGGTCTTG+AGG	-	chr7.3:57716467-57716486	None:intergenic	50.0%
	CACGGTCTTGGTCAGAGATG+AGG	-	chr7.3:57716443-57716462	None:intergenic	55.0%
	CTCATCTCTGACCAAGACCG+TGG	+	chr7.3:57716441-57716460	MS.gene38327:CDS	55.0%
	CTTGGTCAGAGATGAGGGTG+AGG	-	chr7.3:57716437-57716456	None:intergenic	55.0%
	GCAACCGCCAAGTTGTCGAT+AGG	-	chr7.3:57716566-57716585	None:intergenic	55.0%
	GGGGAAGAAAAGGGAAGTGG+TGG	-	chr7.3:57716361-57716380	None:intergenic	55.0%
!	CGATCGACACTAGCTTGGTG+GGG	-	chr7.3:57716485-57716504	None:intergenic	55.0%
!!	GGCATCGGTACAGTAACCAC+TGG	+	chr7.3:57716534-57716553	MS.gene38327:CDS	55.0%
	CGACAACTTGGCGGTTGCTC+TGG	+	chr7.3:57716568-57716587	MS.gene38327:CDS	60.0%
	GGTCAGAGATGAGGGTGAGG+AGG	-	chr7.3:57716434-57716453	None:intergenic	60.0%
!!	GAAAAGGGAAGTGGTGGCGG+TGG	-	chr7.3:57716355-57716374	None:intergenic	60.0%
	GGGTCTTGAGGCCACGGTCT+TGG	-	chr7.3:57716455-57716474	None:intergenic	65.0%
!!	AAGGGAAGTGGTGGCGGTGG+TGG	-	chr7.3:57716352-57716371	None:intergenic	65.0%
!!	AGTGGTGGCGGTGGTGGTGG+TGG	-	chr7.3:57716346-57716365	None:intergenic	70.0%
!!	GGAAGTGGTGGCGGTGGTGG+TGG	-	chr7.3:57716349-57716368	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	57716324	57716806	57716324	ID=MS.gene38327
chr7.3	mRNA	57716324	57716806	57716324	ID=MS.gene38327.t1;Parent=MS.gene38327
chr7.3	exon	57716324	57716806	57716324	ID=MS.gene38327.t1.exon1;Parent=MS.gene38327.t1
chr7.3	CDS	57716324	57716806	57716324	ID=cds.MS.gene38327.t1;Parent=MS.gene38327.t1

Gene Sequence

>MS.gene38327

ATGAAACTCAACTCAATCACCACCACCACCACCGCCACCACTTCCCTTTTCTTCCCCAACCCATTCACCTCTCAAACCACCAACAAAAGACTAACCGCCAAAAAGAACCTCCTCACCCTCATCTCTGACCAAGACCGTGGCCTCAAGACCCAAAACAACCCCACCAAGCTAGTGTCGATCGTTACCGTGATCGATTCAATGGTCAAGCTTGGCATCGGTACAGTAACCACTGGTGATTCCCTATCGACAACTTGGCGGTTGCTCTGGACTACTGAGAAGGAGCAGTTGTTCATCATTAAGAAGGCTGGATTGTTTGGAACTAAAGCTGGAGATGTTTTACAGGTTATTGATGTGAAGAATTTGAGTCTTAACAATGTTATTACTTTTCCTCCTGATGGTTTTTTTTTTTTTAGAAAAAATGGTGTTTTCTTTGTTCGGTCGAATATTGAAGTTGCTTCGGAGCAGAGAGTGAACTTCAAGTGA

Protein sequence

>MS.gene38327.t1

MKLNSITTTTTATTSLFFPNPFTSQTTNKRLTAKKNLLTLISDQDRGLKTQNNPTKLVSIVTVIDSMVKLGIGTVTTGDSLSTTWRLLWTTEKEQLFIIKKAGLFGTKAGDVLQVIDVKNLSLNNVITFPPDGFFFFRKNGVFFVRSNIEVASEQRVNFK