Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38679.t1 | XP_013461725.1 | 94.9 | 178 | 5 | 1 | 1 | 178 | 1 | 174 | 1.80E-81 | 312 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38679.t1 | Q9STX3 | 60.0 | 155 | 39 | 1 | 24 | 178 | 16 | 147 | 1.5e-44 | 180.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38679.t1 | I3T3Z8 | 94.9 | 178 | 5 | 1 | 1 | 178 | 1 | 174 | 1.3e-81 | 312.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene38679 | 0.808398 | 3.16E-50 | -1.69E-46 |
| MS.gene051201 | MS.gene38679 | 0.831563 | 1.56E-55 | -1.69E-46 |
| MS.gene051279 | MS.gene38679 | 0.821945 | 3.08E-53 | -1.69E-46 |
| MS.gene052016 | MS.gene38679 | 0.802753 | 4.83E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene38679 | 0.821203 | 4.58E-53 | -1.69E-46 |
| MS.gene054178 | MS.gene38679 | 0.818932 | 1.51E-52 | -1.69E-46 |
| MS.gene056677 | MS.gene38679 | 0.800435 | 1.44E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene38679 | 0.809741 | 1.63E-50 | -1.69E-46 |
| MS.gene058289 | MS.gene38679 | 0.81537 | 9.57E-52 | -1.69E-46 |
| MS.gene059965 | MS.gene38679 | 0.819679 | 1.02E-52 | -1.69E-46 |
| MS.gene060373 | MS.gene38679 | 0.805139 | 1.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38679.t1 | MTR_3g102470 | 94.944 | 178 | 5 | 1 | 1 | 178 | 1 | 174 | 3.66e-123 | 344 |
| MS.gene38679.t1 | MTR_7g014710 | 90.000 | 40 | 4 | 0 | 1 | 40 | 1 | 40 | 1.32e-17 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38679.t1 | AT4G24210 | 60.000 | 155 | 39 | 1 | 24 | 178 | 16 | 147 | 1.59e-61 | 187 |
| MS.gene38679.t1 | AT5G48170 | 28.346 | 127 | 66 | 3 | 45 | 171 | 32 | 133 | 1.96e-11 | 59.7 |
Find 21 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTAAGCCTTTACCTCGTT+TGG | 0.232824 | 3.3:-84685204 | MS.gene38679:CDS |
| GACCTTATCACTTGTGGTAT+TGG | 0.306662 | 3.3:+84685224 | None:intergenic |
| CGATCTGTTGTTCTTGCTCT+TGG | 0.347661 | 3.3:-84685341 | MS.gene38679:CDS |
| CATGTTCTTCTTCATCATCA+TGG | 0.373154 | 3.3:-84685250 | MS.gene38679:CDS |
| TCTAAGCCTTTACCTCGTTT+GGG | 0.384908 | 3.3:-84685203 | MS.gene38679:CDS |
| CTGCACAAGATGAGAGACTT+TGG | 0.406241 | 3.3:-84685418 | MS.gene38679:CDS |
| ATGTTCTTCTTCATCATCAT+GGG | 0.407721 | 3.3:-84685249 | MS.gene38679:CDS |
| TCTGTTGTTCTTGCTCTTGG+TGG | 0.449708 | 3.3:-84685338 | MS.gene38679:CDS |
| TGCACAAGATGAGAGACTTT+GGG | 0.489559 | 3.3:-84685417 | MS.gene38679:CDS |
| GTCCAATACCACAAGTGATA+AGG | 0.497370 | 3.3:-84685226 | MS.gene38679:CDS |
| GGCTTAGACCTTATCACTTG+TGG | 0.534034 | 3.3:+84685218 | None:intergenic |
| GATGTTTGAGGCTTTGAAAG+AGG | 0.571852 | 3.3:+84685284 | None:intergenic |
| CAGTGGGCTAACACTGGCTG+TGG | 0.598913 | 3.3:-84685377 | MS.gene38679:CDS |
| TGTTCTTCTTCATCATCATG+GGG | 0.610249 | 3.3:-84685248 | MS.gene38679:CDS |
| ACAAAACAGTGGGCTAACAC+TGG | 0.611464 | 3.3:-84685383 | MS.gene38679:CDS |
| ACCTCGTTTGGGAAAAGACG+AGG | 0.618915 | 3.3:-84685192 | MS.gene38679:CDS |
| GCTGATCTGTACAAAACAGT+GGG | 0.622628 | 3.3:-84685393 | MS.gene38679:CDS |
| GGGGTTTGTGAGAAGAATCG+AGG | 0.643342 | 3.3:-84685542 | MS.gene38679:CDS |
| AGCTGATCTGTACAAAACAG+TGG | 0.655674 | 3.3:-84685394 | MS.gene38679:CDS |
| GGGTTTGTGAGAAGAATCGA+GGG | 0.660517 | 3.3:-84685541 | MS.gene38679:CDS |
| CTAGTTGTGTGAACAAACAA+TGG | 0.693160 | 3.3:-84685448 | MS.gene38679:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAGTGAAAAAATTGAT+GGG | - | chr3.3:84685194-84685213 | MS.gene38679:CDS | 20.0% |
| ! | AAACAGTGAAAAAATTGATG+GGG | - | chr3.3:84685195-84685214 | MS.gene38679:CDS | 25.0% |
| ! | GAAAACAGTGAAAAAATTGA+TGG | - | chr3.3:84685193-84685212 | MS.gene38679:CDS | 25.0% |
| ATGTTCTTCTTCATCATCAT+GGG | - | chr3.3:84685507-84685526 | MS.gene38679:CDS | 30.0% | |
| TGAAGACAGAAAAATGAAGA+GGG | - | chr3.3:84685150-84685169 | MS.gene38679:CDS | 30.0% | |
| CATGTTCTTCTTCATCATCA+TGG | - | chr3.3:84685506-84685525 | MS.gene38679:CDS | 35.0% | |
| CTAGTTGTGTGAACAAACAA+TGG | - | chr3.3:84685308-84685327 | MS.gene38679:CDS | 35.0% | |
| GTGAAGACAGAAAAATGAAG+AGG | - | chr3.3:84685149-84685168 | MS.gene38679:CDS | 35.0% | |
| TGTTCTTCTTCATCATCATG+GGG | - | chr3.3:84685508-84685527 | MS.gene38679:CDS | 35.0% | |
| ! | GATGATGATGATGATGTTTG+AGG | + | chr3.3:84685487-84685506 | None:intergenic | 35.0% |
| !! | CTTCATTTTTCTGTCTTCAC+TGG | + | chr3.3:84685149-84685168 | None:intergenic | 35.0% |
| AGCTGATCTGTACAAAACAG+TGG | - | chr3.3:84685362-84685381 | MS.gene38679:CDS | 40.0% | |
| CAGAAAAATGAAGAGGGTGA+AGG | - | chr3.3:84685156-84685175 | MS.gene38679:CDS | 40.0% | |
| GACCTTATCACTTGTGGTAT+TGG | + | chr3.3:84685535-84685554 | None:intergenic | 40.0% | |
| GCTGATCTGTACAAAACAGT+GGG | - | chr3.3:84685363-84685382 | MS.gene38679:CDS | 40.0% | |
| GTCCAATACCACAAGTGATA+AGG | - | chr3.3:84685530-84685549 | MS.gene38679:CDS | 40.0% | |
| TCTAAGCCTTTACCTCGTTT+GGG | - | chr3.3:84685553-84685572 | MS.gene38679:CDS | 40.0% | |
| TGCACAAGATGAGAGACTTT+GGG | - | chr3.3:84685339-84685358 | MS.gene38679:CDS | 40.0% | |
| ! | TCTTTTCCCAAACGAGGTAA+AGG | + | chr3.3:84685562-84685581 | None:intergenic | 40.0% |
| !! | GATGTTTGAGGCTTTGAAAG+AGG | + | chr3.3:84685475-84685494 | None:intergenic | 40.0% |
| CGATCTGTTGTTCTTGCTCT+TGG | - | chr3.3:84685415-84685434 | MS.gene38679:CDS | 45.0% | |
| CTGCACAAGATGAGAGACTT+TGG | - | chr3.3:84685338-84685357 | MS.gene38679:CDS | 45.0% | |
| GGCTTAGACCTTATCACTTG+TGG | + | chr3.3:84685541-84685560 | None:intergenic | 45.0% | |
| GGGTTTGTGAGAAGAATCGA+GGG | - | chr3.3:84685215-84685234 | MS.gene38679:CDS | 45.0% | |
| GTCTAAGCCTTTACCTCGTT+TGG | - | chr3.3:84685552-84685571 | MS.gene38679:CDS | 45.0% | |
| TCTGTTGTTCTTGCTCTTGG+TGG | - | chr3.3:84685418-84685437 | MS.gene38679:CDS | 45.0% | |
| !! | ACAAAACAGTGGGCTAACAC+TGG | - | chr3.3:84685373-84685392 | MS.gene38679:CDS | 45.0% |
| ACCTCGTTTGGGAAAAGACG+AGG | - | chr3.3:84685564-84685583 | MS.gene38679:CDS | 50.0% | |
| GGGGTTTGTGAGAAGAATCG+AGG | - | chr3.3:84685214-84685233 | MS.gene38679:CDS | 50.0% | |
| ! | ACCTCGTCTTTTCCCAAACG+AGG | + | chr3.3:84685568-84685587 | None:intergenic | 50.0% |
| !! | CAGTGGGCTAACACTGGCTG+TGG | - | chr3.3:84685379-84685398 | MS.gene38679:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 84685121 | 84685657 | 84685121 | ID=MS.gene38679 |
| chr3.3 | mRNA | 84685121 | 84685657 | 84685121 | ID=MS.gene38679.t1;Parent=MS.gene38679 |
| chr3.3 | exon | 84685121 | 84685657 | 84685121 | ID=MS.gene38679.t1.exon1;Parent=MS.gene38679.t1 |
| chr3.3 | CDS | 84685121 | 84685657 | 84685121 | ID=cds.MS.gene38679.t1;Parent=MS.gene38679.t1 |
| Gene Sequence |
| Protein sequence |
