Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39510.t1 | RHN62374.1 | 91 | 277 | 25 | 0 | 231 | 507 | 1 | 277 | 4.90E-132 | 481.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39510.t1 | Q688X9 | 54.7 | 203 | 83 | 4 | 1 | 200 | 1 | 197 | 6.1e-51 | 203.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39510.t1 | A0A396IDQ2 | 91.0 | 277 | 25 | 0 | 231 | 507 | 1 | 277 | 3.5e-132 | 481.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049983 | MS.gene39510 | 0.81068 | 1.02E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene39510 | 0.819713 | 1.01E-52 | -1.69E-46 |
| MS.gene050941 | MS.gene39510 | 0.823648 | 1.24E-53 | -1.69E-46 |
| MS.gene051856 | MS.gene39510 | 0.802549 | 5.32E-49 | -1.69E-46 |
| MS.gene056181 | MS.gene39510 | 0.806003 | 1.02E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene39510 | -0.817854 | 2.66E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene39510 | 0.821853 | 3.24E-53 | -1.69E-46 |
| MS.gene060259 | MS.gene39510 | 0.801115 | 1.05E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene39510 | 0.817608 | 3.02E-52 | -1.69E-46 |
| MS.gene06121 | MS.gene39510 | 0.806481 | 8.06E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39510.t1 | MTR_3g019330 | 84.155 | 284 | 45 | 0 | 219 | 502 | 43 | 326 | 5.25e-171 | 490 |
| MS.gene39510.t1 | MTR_4g088440 | 90.875 | 263 | 24 | 0 | 245 | 507 | 1 | 263 | 4.82e-159 | 452 |
| MS.gene39510.t1 | MTR_4g088430 | 85.204 | 196 | 4 | 3 | 1 | 195 | 1 | 172 | 2.78e-112 | 331 |
| MS.gene39510.t1 | MTR_4g088440 | 91.848 | 184 | 15 | 0 | 231 | 414 | 1 | 184 | 4.31e-103 | 307 |
| MS.gene39510.t1 | MTR_1g083780 | 45.312 | 128 | 64 | 2 | 31 | 157 | 30 | 152 | 1.38e-29 | 116 |
| MS.gene39510.t1 | MTR_2g035070 | 32.704 | 159 | 84 | 4 | 40 | 185 | 38 | 186 | 5.58e-19 | 86.3 |
| MS.gene39510.t1 | MTR_7g112400 | 39.726 | 73 | 43 | 1 | 29 | 101 | 46 | 117 | 1.93e-14 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39510.t1 | AT1G50910 | 55.600 | 250 | 107 | 3 | 233 | 480 | 4 | 251 | 3.52e-72 | 238 |
| MS.gene39510.t1 | AT3G05700 | 39.614 | 207 | 107 | 7 | 1 | 198 | 1 | 198 | 1.38e-35 | 132 |
| MS.gene39510.t1 | AT3G05700 | 39.706 | 204 | 105 | 7 | 1 | 195 | 1 | 195 | 2.31e-35 | 132 |
| MS.gene39510.t1 | AT5G26990 | 36.453 | 203 | 112 | 7 | 1 | 195 | 1 | 194 | 1.56e-29 | 116 |
| MS.gene39510.t1 | AT5G49230 | 41.667 | 132 | 69 | 3 | 31 | 162 | 28 | 151 | 2.42e-26 | 106 |
| MS.gene39510.t1 | AT1G56280 | 42.188 | 128 | 65 | 4 | 31 | 157 | 20 | 139 | 7.44e-25 | 102 |
| MS.gene39510.t1 | AT5G49230 | 40.146 | 137 | 69 | 4 | 31 | 162 | 28 | 156 | 1.23e-24 | 102 |
| MS.gene39510.t1 | AT1G56280 | 42.188 | 128 | 64 | 5 | 31 | 157 | 27 | 145 | 2.24e-23 | 98.2 |
| MS.gene39510.t1 | AT3G06760 | 36.478 | 159 | 80 | 3 | 32 | 187 | 33 | 173 | 8.39e-23 | 97.4 |
| MS.gene39510.t1 | AT1G50910 | 46.000 | 100 | 50 | 3 | 383 | 480 | 11 | 108 | 1.70e-16 | 81.6 |
| MS.gene39510.t1 | AT4G02200 | 32.812 | 128 | 74 | 4 | 35 | 162 | 32 | 147 | 8.30e-16 | 76.6 |
| MS.gene39510.t1 | AT4G02200 | 32.812 | 128 | 74 | 4 | 35 | 162 | 32 | 147 | 9.97e-16 | 76.6 |
| MS.gene39510.t1 | AT4G02200 | 30.675 | 163 | 84 | 7 | 35 | 180 | 32 | 182 | 1.39e-13 | 70.9 |
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACACCTGTACAACATATT+TGG | 0.197201 | 4.2:-22759893 | MS.gene39510:CDS |
| GAAAGTCAAGTTGAGGATTT+TGG | 0.209491 | 4.2:-22752256 | MS.gene39510:CDS |
| CAGTCAGACGTTGTCTCTTT+TGG | 0.211711 | 4.2:-22760114 | MS.gene39510:CDS |
| TAGATGAGAGGAAGCATAAA+AGG | 0.211889 | 4.2:-22752285 | MS.gene39510:CDS |
| AATCACCCAACTTCTCATTT+AGG | 0.253905 | 4.2:-22761766 | MS.gene39510:intron |
| TACATCGCAGCCTTCAAAAT+TGG | 0.278836 | 4.2:+22753693 | None:intergenic |
| CATTTGCAGGTGCTTCTTAA+TGG | 0.280797 | 4.2:-22760071 | MS.gene39510:CDS |
| CTTCAAAAGATGCTGGAAAT+AGG | 0.288940 | 4.2:+22755703 | None:intergenic |
| AACACTATTGAGTCGATTCT+TGG | 0.308082 | 4.2:-22752196 | MS.gene39510:CDS |
| AAAAGACGAATCTTCTGTTT+TGG | 0.324292 | 4.2:+22755361 | None:intergenic |
| CTTCAATGGATTCTGAGTTC+TGG | 0.334015 | 4.2:-22761834 | None:intergenic |
| TGTTTCATGACAGCTTAAAA+AGG | 0.336884 | 4.2:-22754680 | MS.gene39510:intron |
| GCTAGCGCGTCTACGAGTTT+TGG | 0.353240 | 4.2:-22752866 | MS.gene39510:CDS |
| CAATAAGGACAAGGGAAATC+TGG | 0.353737 | 4.2:+22761529 | None:intergenic |
| TCAAACTCTTCATAACAATA+AGG | 0.379403 | 4.2:+22761514 | None:intergenic |
| AGAGACAACGTCTGACTGTT+TGG | 0.384231 | 4.2:+22760119 | None:intergenic |
| CTTCTTAATGGTGGTGGTGG+TGG | 0.390196 | 4.2:-22760059 | MS.gene39510:CDS |
| ACATCTACTTCACCACTCTC+TGG | 0.392256 | 4.2:+22751460 | None:intergenic |
| ACACTATTGAGTCGATTCTT+GGG | 0.399329 | 4.2:-22752195 | MS.gene39510:CDS |
| TCTACGTGAAGCTCATTTGC+AGG | 0.400989 | 4.2:-22760084 | MS.gene39510:CDS |
| TGTCGCAAACTAGGCGATGC+TGG | 0.403310 | 4.2:+22758915 | None:intergenic |
| TACAAACCTAAATGAGAAGT+TGG | 0.412351 | 4.2:+22761760 | None:intergenic |
| ATGTTACATTAGAGTGTGTC+TGG | 0.424478 | 4.2:-22752327 | MS.gene39510:CDS |
| AGATGAGAGGAAGCATAAAA+GGG | 0.441741 | 4.2:-22752284 | MS.gene39510:CDS |
| GATTTCCAAATATGTTGTAC+AGG | 0.448069 | 4.2:+22759888 | None:intergenic |
| GGGACTTTCTGTGACAATCA+TGG | 0.457633 | 4.2:-22752893 | MS.gene39510:CDS |
| ATTTCTTCTGCTTCGCATGC+AGG | 0.460602 | 4.2:+22759963 | None:intergenic |
| TACAACATATTTGGAAATCA+AGG | 0.462735 | 4.2:-22759884 | MS.gene39510:intron |
| CAATCTATTAAAATAACTCA+TGG | 0.479405 | 4.2:-22752541 | MS.gene39510:CDS |
| GTGCTTCTTAATGGTGGTGG+TGG | 0.480850 | 4.2:-22760062 | MS.gene39510:CDS |
| GTCGCAAACTAGGCGATGCT+GGG | 0.482220 | 4.2:+22758916 | None:intergenic |
| CAGGTGCTTCTTAATGGTGG+TGG | 0.492363 | 4.2:-22760065 | MS.gene39510:CDS |
| ATTAAATTGCAAGGATTCGA+TGG | 0.492656 | 4.2:-22755843 | MS.gene39510:CDS |
| GGATTTAAATCTTCTACAGT+TGG | 0.499368 | 4.2:-22755437 | MS.gene39510:CDS |
| ATGTGGAATACTTGAAAGAT+TGG | 0.505870 | 4.2:-22755759 | MS.gene39510:CDS |
| AGTAGATGTTGCAGCTAAAG+AGG | 0.506345 | 4.2:-22751446 | MS.gene39510:CDS |
| TGTTATGAAGAGTTTGACAT+AGG | 0.509197 | 4.2:-22761508 | MS.gene39510:CDS |
| CCACCAGGTTGCTGCTATGT+TGG | 0.509268 | 4.2:+22758890 | None:intergenic |
| TGCATACCTTCAAAAGATGC+TGG | 0.509973 | 4.2:+22755696 | None:intergenic |
| AATCTATTAAAATAACTCAT+GGG | 0.513875 | 4.2:-22752540 | MS.gene39510:CDS |
| ATGTTGGAATGTCGCAAACT+AGG | 0.518756 | 4.2:+22758906 | None:intergenic |
| CATCTACTTCACCACTCTCT+GGG | 0.521488 | 4.2:+22751461 | None:intergenic |
| ACATATCAGAATTATATCCT+TGG | 0.521831 | 4.2:+22751411 | None:intergenic |
| ACAAACCTAAATGAGAAGTT+GGG | 0.527430 | 4.2:+22761761 | None:intergenic |
| ATCCTTGCAATTTAATCCAA+CGG | 0.534772 | 4.2:+22755850 | None:intergenic |
| TTCATGTGAATCAAGAGTTA+CGG | 0.536211 | 4.2:-22761455 | MS.gene39510:intron |
| GAATTATATCCTTGGCATGA+GGG | 0.538937 | 4.2:+22751419 | None:intergenic |
| ATCTATTAAAATAACTCATG+GGG | 0.540345 | 4.2:-22752539 | MS.gene39510:CDS |
| GTCCGTGTTGCAGCGTTATG+TGG | 0.541849 | 4.2:+22760292 | None:intergenic |
| ACAATGGAGCTGCGAAAGCA+TGG | 0.545280 | 4.2:+22755784 | None:intergenic |
| GTAGATCATAGTTCTGCCGT+TGG | 0.547170 | 4.2:-22755866 | MS.gene39510:intron |
| CACTATTGAGTCGATTCTTG+GGG | 0.548086 | 4.2:-22752194 | MS.gene39510:CDS |
| TCGCAGCTCCATTGTGAATG+TGG | 0.550317 | 4.2:-22755776 | MS.gene39510:CDS |
| TCATGTTTCAATGTTATCAA+AGG | 0.550576 | 4.2:-22755391 | MS.gene39510:CDS |
| AAAATAAAAGAGGATAGGAA+TGG | 0.553629 | 4.2:+22759993 | None:intergenic |
| TGCCGTTGGATTAAATTGCA+AGG | 0.554378 | 4.2:-22755852 | MS.gene39510:CDS |
| GAATGGATCAGCAGACGCAT+TGG | 0.556423 | 4.2:+22760010 | None:intergenic |
| AGGGTAATTCAAAATAAAAG+AGG | 0.557118 | 4.2:+22759983 | None:intergenic |
| CACCACTCTCTGGGTCAAGT+TGG | 0.559539 | 4.2:+22751470 | None:intergenic |
| CAAGTATTCCACATTCACAA+TGG | 0.564121 | 4.2:+22755768 | None:intergenic |
| AATTATATCCTTGGCATGAG+GGG | 0.564514 | 4.2:+22751420 | None:intergenic |
| TTTCTTCTGCTTCGCATGCA+GGG | 0.565302 | 4.2:+22759964 | None:intergenic |
| GCAACTTTAACTGAGCATAC+AGG | 0.565487 | 4.2:+22760330 | None:intergenic |
| AATTCAAAATAAAAGAGGAT+AGG | 0.565902 | 4.2:+22759988 | None:intergenic |
| TAATCATTATCATCACTACC+AGG | 0.567418 | 4.2:+22752349 | None:intergenic |
| ATAAGTATATATTAGATGAG+AGG | 0.567492 | 4.2:-22752297 | MS.gene39510:CDS |
| GGAGAATGAAAGTCAAGTTG+AGG | 0.573104 | 4.2:-22752263 | MS.gene39510:CDS |
| GTTTGGTCATTAGGTGCGGA+GGG | 0.573872 | 4.2:-22755458 | MS.gene39510:intron |
| AAGCGTATAGTGGCGCTTAG+CGG | 0.574792 | 4.2:+22761795 | None:intergenic |
| TTGCAGGTGCTTCTTAATGG+TGG | 0.575859 | 4.2:-22760068 | MS.gene39510:CDS |
| TTAAATTGCAAGGATTCGAT+GGG | 0.589402 | 4.2:-22755842 | MS.gene39510:CDS |
| GATTGTGTTGAAGCGTATAG+TGG | 0.590099 | 4.2:+22761785 | None:intergenic |
| TTACTGTGTTACTGTGCGAC+CGG | 0.593038 | 4.2:+22760033 | None:intergenic |
| ATTCCAACATAGCAGCAACC+TGG | 0.600948 | 4.2:-22758893 | MS.gene39510:CDS |
| CACCAACGTACATGACCACC+AGG | 0.604686 | 4.2:+22758875 | None:intergenic |
| GGTTTGGTCATTAGGTGCGG+AGG | 0.608260 | 4.2:-22755459 | MS.gene39510:intron |
| TACTGAATTTGAAACAGTCG+AGG | 0.609403 | 4.2:-22755819 | MS.gene39510:CDS |
| AGAATTATATCCTTGGCATG+AGG | 0.615696 | 4.2:+22751418 | None:intergenic |
| GAAAATGCCTTGATGAAAAG+CGG | 0.616892 | 4.2:-22754656 | MS.gene39510:CDS |
| AGAAGAAATCTCTAAATCTG+TGG | 0.631495 | 4.2:-22759949 | MS.gene39510:CDS |
| GACCAACTTGACCCAGAGAG+TGG | 0.632334 | 4.2:-22751472 | MS.gene39510:CDS |
| ATTATATCCTTGGCATGAGG+GGG | 0.638226 | 4.2:+22751421 | None:intergenic |
| TCTACAGTTGGCAAACGTAG+AGG | 0.644321 | 4.2:-22755425 | MS.gene39510:CDS |
| TCGCAAACTAGGCGATGCTG+GGG | 0.647766 | 4.2:+22758917 | None:intergenic |
| ATTACATACAGAGAAGGCGC+AGG | 0.648279 | 4.2:-22760160 | MS.gene39510:intron |
| CTTCATAACAATAAGGACAA+GGG | 0.652964 | 4.2:+22761521 | None:intergenic |
| CTTAGCGGCAGCAAGACGAG+AGG | 0.656188 | 4.2:+22761810 | None:intergenic |
| TCTTCATAACAATAAGGACA+AGG | 0.656421 | 4.2:+22761520 | None:intergenic |
| ACTATTGAGTCGATTCTTGG+GGG | 0.665684 | 4.2:-22752193 | MS.gene39510:intron |
| ATCTGCACGACTACTAGCAG+AGG | 0.666792 | 4.2:-22754610 | MS.gene39510:intron |
| GATGCTGGAAATAGGAAGAG+CGG | 0.678399 | 4.2:+22755711 | None:intergenic |
| AATTTGAAACAGTCGAGGGG+CGG | 0.679861 | 4.2:-22755814 | MS.gene39510:CDS |
| AGCCACATAACGCTGCAACA+CGG | 0.680174 | 4.2:-22760294 | MS.gene39510:CDS |
| TCTATTAAAATAACTCATGG+GGG | 0.690281 | 4.2:-22752538 | MS.gene39510:CDS |
| ACTGAATTTGAAACAGTCGA+GGG | 0.690372 | 4.2:-22755818 | MS.gene39510:CDS |
| AATCATTATCATCACTACCA+GGG | 0.697644 | 4.2:+22752350 | None:intergenic |
| CTGAATTTGAAACAGTCGAG+GGG | 0.699477 | 4.2:-22755817 | MS.gene39510:CDS |
| GCTGGAAATAGGAAGAGCGG+CGG | 0.714092 | 4.2:+22755714 | None:intergenic |
| CCAACATAGCAGCAACCTGG+TGG | 0.728692 | 4.2:-22758890 | MS.gene39510:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 22751408 | 22761851 | 22751408 | ID=MS.gene39510 |
| chr4.2 | mRNA | 22751408 | 22761851 | 22751408 | ID=MS.gene39510.t1;Parent=MS.gene39510 |
| chr4.2 | exon | 22761767 | 22761851 | 22761767 | ID=MS.gene39510.t1.exon1;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22761767 | 22761851 | 22761767 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22761456 | 22761595 | 22761456 | ID=MS.gene39510.t1.exon2;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22761456 | 22761595 | 22761456 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22760288 | 22760358 | 22760288 | ID=MS.gene39510.t1.exon3;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22760288 | 22760358 | 22760288 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22759885 | 22760169 | 22759885 | ID=MS.gene39510.t1.exon4;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22759885 | 22760169 | 22759885 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22758886 | 22758956 | 22758886 | ID=MS.gene39510.t1.exon5;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22758886 | 22758956 | 22758886 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22755703 | 22755884 | 22755703 | ID=MS.gene39510.t1.exon6;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22755703 | 22755884 | 22755703 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22755355 | 22755467 | 22755355 | ID=MS.gene39510.t1.exon7;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22755355 | 22755467 | 22755355 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22754611 | 22754689 | 22754611 | ID=MS.gene39510.t1.exon8;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22754611 | 22754689 | 22754611 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22753696 | 22753724 | 22753696 | ID=MS.gene39510.t1.exon9;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22753696 | 22753724 | 22753696 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22752858 | 22752951 | 22752858 | ID=MS.gene39510.t1.exon10;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22752858 | 22752951 | 22752858 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22752531 | 22752620 | 22752531 | ID=MS.gene39510.t1.exon11;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22752531 | 22752620 | 22752531 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22752194 | 22752389 | 22752194 | ID=MS.gene39510.t1.exon12;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22752194 | 22752389 | 22752194 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| chr4.2 | exon | 22751408 | 22751496 | 22751408 | ID=MS.gene39510.t1.exon13;Parent=MS.gene39510.t1 |
| chr4.2 | CDS | 22751408 | 22751496 | 22751408 | ID=cds.MS.gene39510.t1;Parent=MS.gene39510.t1 |
| Gene Sequence |
| Protein sequence |
