AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39553

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39553.t1 MTR_4g087955 93.600 250 16 0 1 250 1 250 8.03e-179 491
MS.gene39553.t1 MTR_1g097870 58.416 101 24 2 150 250 62 144 8.24e-32 114
MS.gene39553.t1 MTR_1g097880 51.064 94 28 4 36 129 2 77 4.69e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39553.t1 AT2G34050 73.206 209 56 0 42 250 40 248 2.96e-118 338

Find 55 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCGGTTGATGGAATTTC+TGG 0.191016 4.2:-23305969 None:intergenic
TATCTTCAACCTCCAGAATT+TGG 0.194257 4.2:+23305854 MS.gene39553:CDS
ATTGAAAATGGTTACAAAAT+AGG 0.236464 4.2:-23308570 None:intergenic
TGCTATGGTTCTCCAAATTC+TGG 0.304753 4.2:-23305866 None:intergenic
CTGAAAATATCACTTCCATT+TGG 0.350306 4.2:+23306124 MS.gene39553:CDS
TGCAGGGGAGTTGCGGTTGA+TGG 0.354027 4.2:-23305978 None:intergenic
TGAAAATATCACTTCCATTT+GGG 0.358159 4.2:+23306125 MS.gene39553:CDS
ACCCCATTTCAGAAAATTGC+AGG 0.375367 4.2:-23305995 None:intergenic
TCCAAGTGAATTTGAGTTCA+AGG 0.383385 4.2:+23308524 MS.gene39553:CDS
GTAAAATGATACAGTATAGT+AGG 0.387097 4.2:-23308343 None:intergenic
CTCCGAGTCAGTCAGCTTGC+TGG 0.395915 4.2:-23308421 None:intergenic
AGCAAGTCTTTATCACTTGC+TGG 0.405466 4.2:+23306498 MS.gene39553:CDS
TCCTTTGCAACTCAAGCCAT+TGG 0.422997 4.2:+23306062 MS.gene39553:CDS
CGTAGATATTGAGAGGCTTA+AGG 0.428584 4.2:+23306092 MS.gene39553:CDS
CAGAAACTGTTGTGAAAGAA+TGG 0.433681 4.2:-23305933 None:intergenic
ACAAAGGAATGACAAAATAT+CGG 0.436244 4.2:-23306622 None:intergenic
CACATAGGTAGAGGTCATAT+TGG 0.436318 4.2:+23306463 MS.gene39553:CDS
ATTCCTTTGTGGAGGGGAAG+TGG 0.451596 4.2:+23306635 MS.gene39553:CDS
TTTCTGAAATGGGGTTCGCT+TGG 0.455854 4.2:+23306003 MS.gene39553:CDS
TATATAACAATGTTTGTTCA+AGG 0.456491 4.2:+23306659 MS.gene39553:CDS
TGACTGACTCGGAGGCGAAA+TGG 0.460162 4.2:+23308430 MS.gene39553:CDS
TATTGAGAGGCTTAAGGATC+GGG 0.466521 4.2:+23306098 MS.gene39553:CDS
TACTGGTCTTGAAGATTACA+AGG 0.470857 4.2:+23308299 MS.gene39553:CDS
ATTTGCAGAGAGCAAGGACT+TGG 0.474512 4.2:+23308371 MS.gene39553:CDS
AGACATTACATCATCCCAAA+TGG 0.489838 4.2:-23306139 None:intergenic
TCCTTGAACTCAAATTCACT+TGG 0.494816 4.2:-23308525 None:intergenic
GGGGTTCGCTTGGATTCTGC+AGG 0.497969 4.2:+23306013 MS.gene39553:CDS
TTGCGGTTGATGGAATTTCT+GGG 0.509138 4.2:-23305968 None:intergenic
TTTCAGTATCACATAGGTAG+AGG 0.509550 4.2:+23306454 MS.gene39553:intron
CACAGAATTTGCAGAGAGCA+AGG 0.512541 4.2:+23308365 MS.gene39553:CDS
AGAAAATTGCAGGGGAGTTG+CGG 0.514104 4.2:-23305985 None:intergenic
TAAAATGATACAGTATAGTA+GGG 0.515987 4.2:-23308342 None:intergenic
TCCAATGGCTTGAGTTGCAA+AGG 0.530974 4.2:-23306063 None:intergenic
AGAAACTGTTGTGAAAGAAT+GGG 0.537123 4.2:-23305932 None:intergenic
CTCGGAGGCGAAATGGCTTG+TGG 0.552401 4.2:+23308437 MS.gene39553:CDS
AGGACTTGGTACTGATCCGT+GGG 0.562824 4.2:+23308385 MS.gene39553:CDS
TTTGTCATTCCTTTGTGGAG+GGG 0.566411 4.2:+23306629 MS.gene39553:CDS
CCCCATTTCAGAAAATTGCA+GGG 0.572888 4.2:-23305994 None:intergenic
ATTCTATCGTAGATATTGAG+AGG 0.578754 4.2:+23306085 MS.gene39553:CDS
ATATTGAGAGGCTTAAGGAT+CGG 0.579057 4.2:+23306097 MS.gene39553:CDS
AGGACGTCAAGATTCGCTAC+TGG 0.579599 4.2:+23306033 MS.gene39553:CDS
TACCAGCAAGCTGACTGACT+CGG 0.584954 4.2:+23308419 MS.gene39553:CDS
TGGAGCATAGAGCTAAAGAA+TGG 0.597620 4.2:+23306518 MS.gene39553:CDS
AAGGACTTGGTACTGATCCG+TGG 0.611446 4.2:+23308384 MS.gene39553:CDS
TAGGCATGTTCAATGCTCGC+AGG 0.614925 4.2:-23308551 None:intergenic
CTTGAAGATTACAAGGCTAG+AGG 0.618380 4.2:+23308306 MS.gene39553:CDS
ATATCTACGATAGAATCCAA+TGG 0.618401 4.2:-23306078 None:intergenic
GAATTTCTGGGAAGTTGCTG+CGG 0.624275 4.2:-23305956 None:intergenic
TATGGTTCTCCAAATTCTGG+AGG 0.630186 4.2:-23305863 None:intergenic
CCCATTTCAGAAAATTGCAG+GGG 0.636308 4.2:-23305993 None:intergenic
TAACCACTTCCCCTCCACAA+AGG 0.668270 4.2:-23306638 None:intergenic
CAGCAAGCTGACTGACTCGG+AGG 0.679284 4.2:+23308422 MS.gene39553:CDS
GTTGTGAAAGAATGGGCATG+AGG 0.724603 4.2:-23305925 None:intergenic
TGGTAAATACAACATCCCCA+CGG 0.737738 4.2:-23308401 None:intergenic
GGACTTGGTACTGATCCGTG+GGG 0.769538 4.2:+23308386 MS.gene39553:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGAAAGTAAAAAAAA+AGG + chr4.2:23306927-23306946 MS.gene39553:intron 15.0%
!! ACAACTTAAAACAAAAAAAT+TGG - chr4.2:23307871-23307890 None:intergenic 15.0%
!! TAATTATATCTTGAAACAAT+TGG + chr4.2:23308042-23308061 MS.gene39553:intron 15.0%
!!! CTTAGTTTTTAACATTTTTA+TGG + chr4.2:23307987-23308006 MS.gene39553:intron 15.0%
!!! TAAATTTGAAGTAAAAATGT+TGG + chr4.2:23307513-23307532 MS.gene39553:intron 15.0%
!! AAAATGTTAAAAACTAAGCA+TGG - chr4.2:23307985-23308004 None:intergenic 20.0%
!! ATATTCCAAAATGATCTTAT+GGG + chr4.2:23307305-23307324 MS.gene39553:intron 20.0%
!! TAAAACAAAAAAATTGGTAC+TGG - chr4.2:23307865-23307884 None:intergenic 20.0%
!! TAAAATGATACAGTATAGTA+GGG - chr4.2:23308345-23308364 None:intergenic 20.0%
!! TATATAACAATGTTTGTTCA+AGG + chr4.2:23306659-23306678 MS.gene39553:CDS 20.0%
!! TATTGTTTACAAATGTTGAT+AGG + chr4.2:23306216-23306235 MS.gene39553:intron 20.0%
!! TGGATTAAAATATTTCAATC+AGG + chr4.2:23307533-23307552 MS.gene39553:intron 20.0%
!! TTGTATCTGTAAGAATATTA+GGG - chr4.2:23307200-23307219 None:intergenic 20.0%
!!! AATTTTTATTGGCAACTATA+GGG + chr4.2:23307832-23307851 MS.gene39553:intron 20.0%
!!! ATATTTTACATAACCTATCA+TGG - chr4.2:23306276-23306295 None:intergenic 20.0%
!!! ATTTAACTTTAACAAAGAAG+GGG + chr4.2:23307433-23307452 MS.gene39553:intron 20.0%
!!! CATTTAACTTTAACAAAGAA+GGG + chr4.2:23307432-23307451 MS.gene39553:intron 20.0%
!!! GTTTTAAAATTCTCATTTCT+GGG - chr4.2:23307688-23307707 None:intergenic 20.0%
!!! TCATTTAACTTTAACAAAGA+AGG + chr4.2:23307431-23307450 MS.gene39553:intron 20.0%
!!! TGTTTTAAAATTCTCATTTC+TGG - chr4.2:23307689-23307708 None:intergenic 20.0%
!!! TTGTTGTTAACTATATAACT+TGG + chr4.2:23306423-23306442 MS.gene39553:intron 20.0%
! AAAATAAAAATCACTGTAGC+AGG + chr4.2:23307759-23307778 MS.gene39553:intron 25.0%
! AAACTTTAACTGATAATAGC+CGG - chr4.2:23307616-23307635 None:intergenic 25.0%
! AAGGAAATTGTTTATGTTGT+AGG + chr4.2:23306956-23306975 MS.gene39553:intron 25.0%
! AAGTACTTCACAAAGTTTAT+TGG - chr4.2:23307094-23307113 None:intergenic 25.0%
! AATTTATTTGAGTCCATGAT+AGG + chr4.2:23306260-23306279 MS.gene39553:intron 25.0%
! ACAAAGGAATGACAAAATAT+CGG - chr4.2:23306625-23306644 None:intergenic 25.0%
! ACATAAACAATTTCCTTGAA+TGG - chr4.2:23306953-23306972 None:intergenic 25.0%
! ACCCATATTTAAATGGAAAA+AGG - chr4.2:23306796-23306815 None:intergenic 25.0%
! AGAAATTGAAAAGTTGAAGT+GGG + chr4.2:23306899-23306918 MS.gene39553:intron 25.0%
! AGGAAATTGTTTATGTTGTA+GGG + chr4.2:23306957-23306976 MS.gene39553:intron 25.0%
! AGTATTGTTAGTATTATGCT+AGG + chr4.2:23306829-23306848 MS.gene39553:intron 25.0%
! ATACTAACAATACTAACATC+TGG - chr4.2:23306823-23306842 None:intergenic 25.0%
! ATTTGAATAAATGACAAGAG+AGG - chr4.2:23306700-23306719 None:intergenic 25.0%
! CATATTCCAAAATGATCTTA+TGG + chr4.2:23307304-23307323 MS.gene39553:intron 25.0%
! CCCATATTTAAATGGAAAAA+GGG - chr4.2:23306795-23306814 None:intergenic 25.0%
! CTATAGTTGCCAATAAAAAT+TGG - chr4.2:23307833-23307852 None:intergenic 25.0%
! CTTGTATCTGTAAGAATATT+AGG - chr4.2:23307201-23307220 None:intergenic 25.0%
! GTAAAATGATACAGTATAGT+AGG - chr4.2:23308346-23308365 None:intergenic 25.0%
! GTATTGTTAGTATTATGCTA+GGG + chr4.2:23306830-23306849 MS.gene39553:intron 25.0%
! TATCTTGAAACAATTGGAAA+TGG + chr4.2:23308048-23308067 MS.gene39553:intron 25.0%
! TATTCCAAAATGATCTTATG+GGG + chr4.2:23307306-23307325 MS.gene39553:intron 25.0%
! TATTGTTAGTATTATGCTAG+GGG + chr4.2:23306831-23306850 MS.gene39553:intron 25.0%
! TCCAAAAAATATGTACCATT+AGG - chr4.2:23307903-23307922 None:intergenic 25.0%
! TGAAAATATCACTTCCATTT+GGG + chr4.2:23306125-23306144 MS.gene39553:CDS 25.0%
! TTATATCATATTGAGATCCA+CGG + chr4.2:23307347-23307366 MS.gene39553:intron 25.0%
! TTTGAATAAATGACAAGAGA+GGG - chr4.2:23306699-23306718 None:intergenic 25.0%
!! ACCCTTTTTCCATTTAAATA+TGG + chr4.2:23306791-23306810 MS.gene39553:intron 25.0%
!! AGTTGTTTCATATTTCCTAA+TGG + chr4.2:23307885-23307904 MS.gene39553:intron 25.0%
!! CAATTTTTATTGGCAACTAT+AGG + chr4.2:23307831-23307850 MS.gene39553:intron 25.0%
!! CCCTTTTTCCATTTAAATAT+GGG + chr4.2:23306792-23306811 MS.gene39553:intron 25.0%
!! CTACAAGTTATTTAACTTCA+AGG - chr4.2:23307590-23307609 None:intergenic 25.0%
!! TTCTTTATTCGTTTTCTCTT+TGG + chr4.2:23306187-23306206 MS.gene39553:intron 25.0%
!! TTTTAAACCTGCTATCATAT+TGG - chr4.2:23308018-23308037 None:intergenic 25.0%
!!! CTTTTTATTTGAATGATGTG+AGG + chr4.2:23308472-23308491 MS.gene39553:CDS 25.0%
!!! GTTTTTTTTCAGTATCACAT+AGG + chr4.2:23306448-23306467 MS.gene39553:intron 25.0%
!!! TCAAATTTTAGTTTTCCTTC+AGG + chr4.2:23307231-23307250 MS.gene39553:intron 25.0%
!!! TCCTAATGGTACATATTTTT+TGG + chr4.2:23307899-23307918 MS.gene39553:intron 25.0%
AAACTGTTCAAACTTCAAAG+TGG + chr4.2:23307642-23307661 MS.gene39553:intron 30.0%
ACAAAGTTTATTGGTAGACA+CGG - chr4.2:23307085-23307104 None:intergenic 30.0%
AGAAACTGTTGTGAAAGAAT+GGG - chr4.2:23305935-23305954 None:intergenic 30.0%
ATATCTACGATAGAATCCAA+TGG - chr4.2:23306081-23306100 None:intergenic 30.0%
ATATTTCAATCAGGAACACA+AGG + chr4.2:23307542-23307561 MS.gene39553:intron 30.0%
ATTCTATCGTAGATATTGAG+AGG + chr4.2:23306085-23306104 MS.gene39553:CDS 30.0%
CTGAAAATATCACTTCCATT+TGG + chr4.2:23306124-23306143 MS.gene39553:CDS 30.0%
GAGAAATTGAAAAGTTGAAG+TGG + chr4.2:23306898-23306917 MS.gene39553:intron 30.0%
GTAAAAAAAAAGGCCATTCA+AGG + chr4.2:23306937-23306956 MS.gene39553:intron 30.0%
GTTATCTTCAATGTGACTAT+GGG + chr4.2:23307800-23307819 MS.gene39553:intron 30.0%
TAAAACATCAACAAGCCTAA+AGG + chr4.2:23307702-23307721 MS.gene39553:intron 30.0%
TTCTATATATCTGATCCTGA+AGG - chr4.2:23307249-23307268 None:intergenic 30.0%
! CAAATGGAAGTGATATTTTC+AGG - chr4.2:23306126-23306145 None:intergenic 30.0%
! GATATTTTGTCATTCCTTTG+TGG + chr4.2:23306624-23306643 MS.gene39553:CDS 30.0%
! GTTAAATAACTTGTAGAGTC+CGG + chr4.2:23307594-23307613 MS.gene39553:intron 30.0%
! TGATAGGTAAAAAAGGCTTT+TGG - chr4.2:23308117-23308136 None:intergenic 30.0%
!! CGAGTTTTGATAGGTAAAAA+AGG - chr4.2:23308124-23308143 None:intergenic 30.0%
!! GTAGATTGTCTATGTTTTGA+TGG + chr4.2:23306372-23306391 MS.gene39553:intron 30.0%
!!! GAAGATTGAGATGTTTTTTC+TGG - chr4.2:23305899-23305918 None:intergenic 30.0%
AAAAATCTCAAGACATTCCG+TGG - chr4.2:23307367-23307386 None:intergenic 35.0%
AAAGTGGCCAATGTCAAAAA+TGG + chr4.2:23307658-23307677 MS.gene39553:intron 35.0%
AATTGAAAAGTTGAAGTGGG+TGG + chr4.2:23306902-23306921 MS.gene39553:intron 35.0%
AGACATTACATCATCCCAAA+TGG - chr4.2:23306142-23306161 None:intergenic 35.0%
AGTCACATTGAAGATAACCA+AGG - chr4.2:23307798-23307817 None:intergenic 35.0%
AGTTGCCAATAAAAATTGGC+AGG - chr4.2:23307829-23307848 None:intergenic 35.0%
ATATTGAGAGGCTTAAGGAT+CGG + chr4.2:23306097-23306116 MS.gene39553:CDS 35.0%
ATTGAAAAGTTGAAGTGGGT+GGG + chr4.2:23306903-23306922 MS.gene39553:intron 35.0%
CAAAGTTTATTGGTAGACAC+GGG - chr4.2:23307084-23307103 None:intergenic 35.0%
CAGAAACTGTTGTGAAAGAA+TGG - chr4.2:23305936-23305955 None:intergenic 35.0%
CAGGATCAGATATATAGAAC+TGG + chr4.2:23307250-23307269 MS.gene39553:intron 35.0%
GGTTATCTTCAATGTGACTA+TGG + chr4.2:23307799-23307818 MS.gene39553:intron 35.0%
GGTTTACATCTCAACTATCA+AGG - chr4.2:23308096-23308115 None:intergenic 35.0%
GTTACATGGAAAAGAGAAAG+CGG + chr4.2:23306979-23306998 MS.gene39553:intron 35.0%
GTTTATGTTGTAGGGTTACA+TGG + chr4.2:23306965-23306984 MS.gene39553:intron 35.0%
TATACTACCCATATCCAAGA+AGG - chr4.2:23306875-23306894 None:intergenic 35.0%
TATCTTCAACCTCCAGAATT+TGG + chr4.2:23305854-23305873 MS.gene39553:CDS 35.0%
TCCTTGAACTCAAATTCACT+TGG - chr4.2:23308528-23308547 None:intergenic 35.0%
TGGCGTTACCCATATTTAAA+TGG - chr4.2:23306803-23306822 None:intergenic 35.0%
TTACATGGAAAAGAGAAAGC+GGG + chr4.2:23306980-23306999 MS.gene39553:intron 35.0%
TTTCAGTATCACATAGGTAG+AGG + chr4.2:23306454-23306473 MS.gene39553:intron 35.0%
! ATTTTGTCATTCCTTTGTGG+AGG + chr4.2:23306627-23306646 MS.gene39553:CDS 35.0%
! GGTGTTGTATGCTAAATAGA+TGG - chr4.2:23307179-23307198 None:intergenic 35.0%
! GGTTTCCTGCCAATTTTTAT+TGG + chr4.2:23307821-23307840 MS.gene39553:intron 35.0%
! TCCAAGTGAATTTGAGTTCA+AGG + chr4.2:23308524-23308543 MS.gene39553:CDS 35.0%
! TTTAACTTCAAGGATAGTGC+AGG - chr4.2:23307580-23307599 None:intergenic 35.0%
! TTTTGTCATTCCTTTGTGGA+GGG + chr4.2:23306628-23306647 MS.gene39553:CDS 35.0%
!! ATAGGAGTCTATGACTTGTT+TGG + chr4.2:23306234-23306253 MS.gene39553:intron 35.0%
!! TACTGGTCTTGAAGATTACA+AGG + chr4.2:23308299-23308318 MS.gene39553:CDS 35.0%
!! TGCTTTGTTCGAGTTTTGAT+AGG - chr4.2:23308133-23308152 None:intergenic 35.0%
!!! TGAGATGTTTTTTCTGGCAT+TGG - chr4.2:23305893-23305912 None:intergenic 35.0%
ACAAATACAGACCAGCAATC+AGG - chr4.2:23306762-23306781 None:intergenic 40.0%
ACACTCAACCTTCTTGGATA+TGG + chr4.2:23306864-23306883 MS.gene39553:intron 40.0%
ACCCCATTTCAGAAAATTGC+AGG - chr4.2:23305998-23306017 None:intergenic 40.0%
AGCAAGTCTTTATCACTTGC+TGG + chr4.2:23306498-23306517 MS.gene39553:CDS 40.0%
ATCGAACAGATCATGACTGA+TGG + chr4.2:23307477-23307496 MS.gene39553:intron 40.0%
CACATAGGTAGAGGTCATAT+TGG + chr4.2:23306463-23306482 MS.gene39553:CDS 40.0%
CACTCAACCTTCTTGGATAT+GGG + chr4.2:23306865-23306884 MS.gene39553:intron 40.0%
CAGGAAAAAAGTTGCTTCCT+TGG + chr4.2:23307778-23307797 MS.gene39553:intron 40.0%
CCCATTTCAGAAAATTGCAG+GGG - chr4.2:23305996-23306015 None:intergenic 40.0%
CCCCATTTCAGAAAATTGCA+GGG - chr4.2:23305997-23306016 None:intergenic 40.0%
CGTAGATATTGAGAGGCTTA+AGG + chr4.2:23306092-23306111 MS.gene39553:CDS 40.0%
CTTGAAGATTACAAGGCTAG+AGG + chr4.2:23308306-23308325 MS.gene39553:CDS 40.0%
GACTTCTATCGAACACCTTT+AGG - chr4.2:23307720-23307739 None:intergenic 40.0%
GATCAAACACTCAACCTTCT+TGG + chr4.2:23306858-23306877 MS.gene39553:intron 40.0%
GGAACTTCCAATATGATAGC+AGG + chr4.2:23308008-23308027 MS.gene39553:intron 40.0%
GGATAGTGCAGGATTTCTAT+TGG - chr4.2:23307569-23307588 None:intergenic 40.0%
GGCTGCAAAAGAATAAGCAA+AGG - chr4.2:23306604-23306623 None:intergenic 40.0%
GTTAGTATTATGCTAGGGGT+GGG + chr4.2:23306835-23306854 MS.gene39553:intron 40.0%
TATGGTTCTCCAAATTCTGG+AGG - chr4.2:23305866-23305885 None:intergenic 40.0%
TATTGAGAGGCTTAAGGATC+GGG + chr4.2:23306098-23306117 MS.gene39553:CDS 40.0%
TCATGATCTGTTCGATTGCA+AGG - chr4.2:23307473-23307492 None:intergenic 40.0%
TGCTATGGTTCTCCAAATTC+TGG - chr4.2:23305869-23305888 None:intergenic 40.0%
TGGAGCATAGAGCTAAAGAA+TGG + chr4.2:23306518-23306537 MS.gene39553:CDS 40.0%
TGGTAAATACAACATCCCCA+CGG - chr4.2:23308404-23308423 None:intergenic 40.0%
TGTTAGTATTATGCTAGGGG+TGG + chr4.2:23306834-23306853 MS.gene39553:intron 40.0%
TGTTGCGAGTGTGATAAGAT+TGG - chr4.2:23307133-23307152 None:intergenic 40.0%
TTAGTATTATGCTAGGGGTG+GGG + chr4.2:23306836-23306855 MS.gene39553:intron 40.0%
TTGGTAGACACGGGAAAATA+AGG - chr4.2:23307075-23307094 None:intergenic 40.0%
TTTGTCATTCCTTTGTGGAG+GGG + chr4.2:23306629-23306648 MS.gene39553:CDS 40.0%
! AGACCAGTAAAAAGCATGTG+CGG - chr4.2:23308288-23308307 None:intergenic 40.0%
! CGATCCCCATAAGATCATTT+TGG - chr4.2:23307313-23307332 None:intergenic 40.0%
!! GGGCAAACCATTTTTGACAT+TGG - chr4.2:23307668-23307687 None:intergenic 40.0%
!! TCCCCTGCAATTTTCTGAAA+TGG + chr4.2:23305992-23306011 MS.gene39553:CDS 40.0%
!! TTGCGGTTGATGGAATTTCT+GGG - chr4.2:23305971-23305990 None:intergenic 40.0%
!! TTTTCTGGCATTGGATGCTA+TGG - chr4.2:23305884-23305903 None:intergenic 40.0%
!!! CCCCTGCAATTTTCTGAAAT+GGG + chr4.2:23305993-23306012 MS.gene39553:CDS 40.0%
!!! CCCTGCAATTTTCTGAAATG+GGG + chr4.2:23305994-23306013 MS.gene39553:CDS 40.0%
AACTGTTTGCACCTGATTGC+TGG + chr4.2:23306748-23306767 MS.gene39553:intron 45.0%
AGAAAATTGCAGGGGAGTTG+CGG - chr4.2:23305988-23306007 None:intergenic 45.0%
ATTTGCAGAGAGCAAGGACT+TGG + chr4.2:23308371-23308390 MS.gene39553:CDS 45.0%
CACAGAATTTGCAGAGAGCA+AGG + chr4.2:23308365-23308384 MS.gene39553:CDS 45.0%
CTTTAACAAAGAAGGGGTCG+TGG + chr4.2:23307439-23307458 MS.gene39553:intron 45.0%
GTTGAAGTGGGTGGGAAAAA+AGG + chr4.2:23306911-23306930 MS.gene39553:intron 45.0%
GTTGTGAAAGAATGGGCATG+AGG - chr4.2:23305928-23305947 None:intergenic 45.0%
TCCTTTGCAACTCAAGCCAT+TGG + chr4.2:23306062-23306081 MS.gene39553:CDS 45.0%
! GAATTTCTGGGAAGTTGCTG+CGG - chr4.2:23305959-23305978 None:intergenic 45.0%
! TCCAATGGCTTGAGTTGCAA+AGG - chr4.2:23306066-23306085 None:intergenic 45.0%
!! GTTGCGGTTGATGGAATTTC+TGG - chr4.2:23305972-23305991 None:intergenic 45.0%
!! TTTCTGAAATGGGGTTCGCT+TGG + chr4.2:23306003-23306022 MS.gene39553:CDS 45.0%
!! AATATATATTAATTAACAAA+TGG + chr4.2:23308168-23308187 MS.gene39553:intron 5.0%
AGGACGTCAAGATTCGCTAC+TGG + chr4.2:23306033-23306052 MS.gene39553:CDS 50.0%
ATTCCTTTGTGGAGGGGAAG+TGG + chr4.2:23306635-23306654 MS.gene39553:CDS 50.0%
GATCTGTTCGATTGCAAGGC+AGG - chr4.2:23307469-23307488 None:intergenic 50.0%
TAACAAAGAAGGGGTCGTGG+TGG + chr4.2:23307442-23307461 MS.gene39553:intron 50.0%
TAACCACTTCCCCTCCACAA+AGG - chr4.2:23306641-23306660 None:intergenic 50.0%
TACCAGCAAGCTGACTGACT+CGG + chr4.2:23308419-23308438 MS.gene39553:CDS 50.0%
TAGGCATGTTCAATGCTCGC+AGG - chr4.2:23308554-23308573 None:intergenic 50.0%
!! AAGGACTTGGTACTGATCCG+TGG + chr4.2:23308384-23308403 MS.gene39553:CDS 50.0%
!! ACGCCGCACATGCTTTTTAC+TGG + chr4.2:23308282-23308301 MS.gene39553:CDS 50.0%
!! AGGACTTGGTACTGATCCGT+GGG + chr4.2:23308385-23308404 MS.gene39553:CDS 50.0%
!! GGACTTGGTACTGATCCGTG+GGG + chr4.2:23308386-23308405 MS.gene39553:CDS 55.0%
!! TGACTGACTCGGAGGCGAAA+TGG + chr4.2:23308430-23308449 MS.gene39553:CDS 55.0%
CTCCGAGTCAGTCAGCTTGC+TGG - chr4.2:23308424-23308443 None:intergenic 60.0%
CTCGGAGGCGAAATGGCTTG+TGG + chr4.2:23308437-23308456 MS.gene39553:CDS 60.0%
GGGGTTCGCTTGGATTCTGC+AGG + chr4.2:23306013-23306032 MS.gene39553:CDS 60.0%
TGCAGGGGAGTTGCGGTTGA+TGG - chr4.2:23305981-23306000 None:intergenic 60.0%
!! CAGCAAGCTGACTGACTCGG+AGG + chr4.2:23308422-23308441 MS.gene39553:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 23305844 23308581 23305844 ID=MS.gene39553
chr4.2 mRNA 23305844 23308581 23305844 ID=MS.gene39553.t1;Parent=MS.gene39553
chr4.2 exon 23305844 23306152 23305844 ID=MS.gene39553.t1.exon1;Parent=MS.gene39553.t1
chr4.2 CDS 23305844 23306152 23305844 ID=cds.MS.gene39553.t1;Parent=MS.gene39553.t1
chr4.2 exon 23306460 23306539 23306460 ID=MS.gene39553.t1.exon2;Parent=MS.gene39553.t1
chr4.2 CDS 23306460 23306539 23306460 ID=cds.MS.gene39553.t1;Parent=MS.gene39553.t1
chr4.2 exon 23306622 23306680 23306622 ID=MS.gene39553.t1.exon3;Parent=MS.gene39553.t1
chr4.2 CDS 23306622 23306680 23306622 ID=cds.MS.gene39553.t1;Parent=MS.gene39553.t1
chr4.2 exon 23308277 23308581 23308277 ID=MS.gene39553.t1.exon4;Parent=MS.gene39553.t1
chr4.2 CDS 23308277 23308581 23308277 ID=cds.MS.gene39553.t1;Parent=MS.gene39553.t1
Gene Sequence

>MS.gene39553

ATGCAAAGAGTATCTTCAACCTCCAGAATTTGGAGAACCATAGCATCCAATGCCAGAAAAAACATCTCAATCTTCAACGAACCTCATGCCCATTCTTTCACAACAGTTTCTGCCGCAGCAACTTCCCAGAAATTCCATCAACCGCAACTCCCCTGCAATTTTCTGAAATGGGGTTCGCTTGGATTCTGCAGGACGTCAAGATTCGCTACTGGCTTCAATCCTTTGCAACTCAAGCCATTGGATTCTATCGTAGATATTGAGAGGCTTAAGGATCGGGATCCTGAAAATATCACTTCCATTTGGGATGATGTAATGTCTTATTTCTCGTAATTTCAATCATTTGTTCTTTATTCGTTTTCTCTTTGGTAATTGTATTGTTTACAAATGTTGATAGGAGTCTATGACTTGTTTGGATTAATTTATTTGAGTCCATGATAGGTTATGTAAAATATTTATAGTTTATATGAAAGTAGCTTATAGCTTTTGTTGTAGAAATAACTTGTATGATTCATTTACATAACTGACTCAGTAGATTGTCTATGTTTTGATGGAATATTGATGTGATTTATGATAATTTCTTTGTTGTTAACTATATAACTTGGCTGTTTTTTTTCAGTATCACATAGGTAGAGGTCATATTGGAGCAACTATGAAAGCAAGTCTTTATCACTTGCTGGAGCATAGAGCTAAAGAATGGTATTCATTTTAATCAATACTTGTTCTGTGAATTTGCGAGCTTCTAGATTTCACATTCTTACCTTTGCTTATTCTTTTGCAGCCGATATTTTGTCATTCCTTTGTGGAGGGGAAGTGGTTATATAACAATGTTTGTTCAAGGTTTGATTAAAGTTACCCTCTCTTGTCATTTATTCAAATGAGTTCAATAGCTGTTAATGTGAATGAAAACTGTTTGCACCTGATTGCTGGTCTGTATTTGTAAAATAGAAACCCTTTTTCCATTTAAATATGGGTAACGCCAGATGTTAGTATTGTTAGTATTATGCTAGGGGTGGGGATCAAACACTCAACCTTCTTGGATATGGGTAGTATATGAGAGAAATTGAAAAGTTGAAGTGGGTGGGAAAAAAGGAAAGTAAAAAAAAAGGCCATTCAAGGAAATTGTTTATGTTGTAGGGTTACATGGAAAAGAGAAAGCGGGATAGAGATGAAGCACATATATTAGTATAATTTAAAAGAGACTCTGAATCGTTGTGATACTTTGCGTTGACCTTATTTTCCCGTGTCTACCAATAAACTTTGTGAAGTACTTGTTCAACTATACGAGTCCAATCTTATCACACTCGCAACATACAGAGAGAATATAGCTTAGCTCCATCTATTTAGCATACAACACCCTAATATTCTTACAGATACAAGAATTTGAGCTTCAAATTTTAGTTTTCCTTCAGGATCAGATATATAGAACTGGATGCTTGATGAAACATTTGACAAACATGAAGCATATTCCAAAATGATCTTATGGGGATCGATCAGTTGTTGAAATTATATCATATTGAGATCCACGGAATGTCTTGAGATTTTTATTTAGAAAAATGCACTTTAAAAATATATCAGTATGTGATTTTCTCATTTAACTTTAACAAAGAAGGGGTCGTGGTGGTCCTGCCTTGCAATCGAACAGATCATGACTGATGGACAACAGAAGATCTAAATTTGAAGTAAAAATGTTGGATTAAAATATTTCAATCAGGAACACAAGGACCAATAGAAATCCTGCACTATCCTTGAAGTTAAATAACTTGTAGAGTCCGGCTATTATCAGTTAAAGTTTTGAAATAAACTGTTCAAACTTCAAAGTGGCCAATGTCAAAAATGGTTTGCCCAGAAATGAGAATTTTAAAACATCAACAAGCCTAAAGGTGTTCGATAGAAGTCTCATGTTTTGTTTAGAAAGAAAATAAAAATCACTGTAGCAGGAAAAAAGTTGCTTCCTTGGTTATCTTCAATGTGACTATGGGTTTCCTGCCAATTTTTATTGGCAACTATAGGGTCAAAAACCAGTACCAATTTTTTTGTTTTAAGTTGTTTCATATTTCCTAATGGTACATATTTTTTGGATAATTAAACAACTGTCAACAGCAAAATGATTATATCTATGTGTTGTGTAATTTTTTAGACCATGCTTAGTTTTTAACATTTTTATGGAACTTCCAATATGATAGCAGGTTTAAAAAATTTAATTATATCTTGAAACAATTGGAAATGGTTTAAATATAAGTGATAGATATCCTTGATAGTTGAGATGTAAACCAAAAGCCTTTTTTACCTATCAAAACTCGAACAAAGCATGTTGAAACAAATATAATATATATTAATTAACAAATGGATATTTTTATTCTGAGTATTTGTGTATGTTAATCTTCTTTACACTTCAATTGATGTCTCACATACATGAGCATTCTTTTCTTGCAGTTCAGACGCCGCACATGCTTTTTACTGGTCTTGAAGATTACAAGGCTAGAGGAACACAAGCATCTCCCTACTATACTGTATCATTTTACACAGAATTTGCAGAGAGCAAGGACTTGGTACTGATCCGTGGGGATGTTGTATTTACCAGCAAGCTGACTGACTCGGAGGCGAAATGGCTTGTGGAAACTGCTCAGTCTTTTTATTTGAATGATGTGAGGTACAAACTAGTCGAGAGATTCAATAAAGATCCAAGTGAATTTGAGTTCAAGGATGTCCTGCGAGCATTGAACATGCCTATTTTGTAA
Protein sequence

>MS.gene39553.t1

MQRVSSTSRIWRTIASNARKNISIFNEPHAHSFTTVSAAATSQKFHQPQLPCNFLKWGSLGFCRTSRFATGFNPLQLKPLDSIVDIERLKDRDPENITSIWDDYHIGRGHIGATMKASLYHLLEHRAKECRYFVIPLWRGSGYITMFVQVQTPHMLFTGLEDYKARGTQASPYYTVSFYTEFAESKDLVLIRGDVVFTSKLTDSEAKWLVETAQSFYLNDVRYKLVERFNKDPSEFEFKDVLRALNMPIL