AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39578

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39578.t1 MTR_4g087490 96.034 353 14 0 12 364 12 364 0.0 692
MS.gene39578.t1 MTR_4g087510 87.912 364 35 2 1 364 1 355 0.0 647
MS.gene39578.t1 MTR_4g087520 82.593 270 47 0 95 364 1 270 4.66e-155 437
MS.gene39578.t1 MTR_5g006340 70.440 318 94 0 47 364 8 325 1.91e-153 435
MS.gene39578.t1 MTR_1g103690 70.440 318 94 0 47 364 8 325 1.11e-152 433
MS.gene39578.t1 MTR_1g081610 71.384 318 91 0 43 360 4 321 1.31e-152 432
MS.gene39578.t1 MTR_1g081620 63.376 314 115 0 47 360 7 320 9.34e-143 407
MS.gene39578.t1 MTR_1g081625 63.376 314 115 0 47 360 7 320 7.05e-138 395
MS.gene39578.t1 MTR_7g087110 57.550 351 136 2 10 360 31 368 5.81e-137 395
MS.gene39578.t1 MTR_7g078070 59.621 317 128 0 46 362 60 376 8.00e-135 390
MS.gene39578.t1 MTR_7g078070 60.323 310 123 0 53 362 7 316 4.05e-133 383
MS.gene39578.t1 MTR_7g078070 60.777 283 111 0 80 362 1 283 2.04e-122 355
MS.gene39578.t1 MTR_1g081640 60.791 278 109 0 47 324 7 284 7.33e-111 327
MS.gene39578.t1 MTR_5g095670 27.887 355 197 6 49 346 46 398 1.75e-38 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39578.t1 AT3G59760 73.352 349 91 1 17 363 81 429 0.0 508
MS.gene39578.t1 AT3G59760 73.352 349 91 1 17 363 81 429 1.10e-180 508
MS.gene39578.t1 AT2G43750 76.636 321 75 0 43 363 71 391 8.12e-177 496
MS.gene39578.t1 AT2G43750 76.636 321 75 0 43 363 71 391 8.12e-177 496
MS.gene39578.t1 AT3G59760 72.586 321 86 1 17 335 81 401 1.88e-161 459
MS.gene39578.t1 AT4G14880 66.246 317 107 0 47 363 5 321 6.20e-148 421
MS.gene39578.t1 AT4G14880 66.246 317 107 0 47 363 5 321 6.20e-148 421
MS.gene39578.t1 AT4G14880 66.246 317 107 0 47 363 5 321 6.20e-148 421
MS.gene39578.t1 AT4G14880 66.246 317 107 0 47 363 5 321 6.20e-148 421
MS.gene39578.t1 AT4G14880 66.246 317 107 0 47 363 5 321 6.20e-148 421
MS.gene39578.t1 AT3G04940 64.151 318 114 0 43 360 4 321 3.01e-139 399
MS.gene39578.t1 AT3G04940 64.151 318 114 0 43 360 4 321 3.01e-139 399
MS.gene39578.t1 AT5G28020 64.151 318 114 0 43 360 3 320 3.40e-138 396
MS.gene39578.t1 AT5G28020 64.151 318 114 0 43 360 3 320 3.40e-138 396
MS.gene39578.t1 AT5G28020 64.151 318 114 0 43 360 3 320 3.40e-138 396
MS.gene39578.t1 AT5G28020 64.151 318 114 0 43 360 3 320 3.40e-138 396
MS.gene39578.t1 AT5G28020 64.151 318 114 0 43 360 3 320 3.40e-138 396
MS.gene39578.t1 AT5G28030 62.617 321 120 0 43 363 3 323 2.97e-135 389
MS.gene39578.t1 AT5G28030 62.617 321 120 0 43 363 3 323 2.97e-135 389
MS.gene39578.t1 AT5G28030 62.617 321 120 0 43 363 3 323 2.97e-135 389
MS.gene39578.t1 AT5G28030 62.617 321 120 0 43 363 3 323 2.97e-135 389
MS.gene39578.t1 AT3G61440 53.760 359 154 4 14 362 8 364 4.12e-128 372
MS.gene39578.t1 AT3G03630 58.438 320 123 1 42 361 94 403 1.59e-122 359
MS.gene39578.t1 AT3G22460 64.074 270 77 1 95 364 1 250 1.01e-119 346
MS.gene39578.t1 AT3G61440 58.582 268 111 0 95 362 1 268 1.54e-110 324
MS.gene39578.t1 AT5G28030 62.753 247 92 0 43 289 3 249 1.24e-104 308
MS.gene39578.t1 AT3G61440 52.443 307 134 4 14 310 8 312 5.91e-103 307
MS.gene39578.t1 AT3G61440 57.851 242 102 0 121 362 2 243 1.76e-97 290
MS.gene39578.t1 AT3G22460 70.166 181 54 0 47 227 8 188 4.58e-93 276
MS.gene39578.t1 AT5G28020 60.256 234 93 0 127 360 1 234 4.97e-91 273
MS.gene39578.t1 AT1G55880 27.717 368 206 6 37 346 30 395 2.35e-40 147
MS.gene39578.t1 AT1G55880 27.187 320 174 5 37 298 30 348 7.73e-35 131

Find 87 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACCGGTGGAACTCTTTC+TGG 0.174287 4.2:+23917577 MS.gene39578:CDS
TGCTGCTACAGCTGCATTGC+AGG 0.226091 4.2:+23918320 MS.gene39578:CDS
TGCTGCTAAGCTTGAGCTTA+TGG 0.244545 4.2:+23916357 MS.gene39578:CDS
CACTAACCAGCAAGCCTTCC+TGG 0.248330 4.2:-23918091 None:intergenic
CACATTCTTCTCGAGCTTCC+TGG 0.248992 4.2:-23918679 None:intergenic
CTCGAAAGCAACATACTTTC+AGG 0.252788 4.2:+23917736 MS.gene39578:CDS
GATTTCTTTATCCAAATTCT+TGG 0.286384 4.2:-23917903 None:intergenic
CACACATAATTCAGGGTATT+GGG 0.300262 4.2:+23917867 MS.gene39578:CDS
GTATGCACTTCAGAGTATTT+TGG 0.302707 4.2:+23917225 MS.gene39578:intron
CACAGGACCACACATAATTC+AGG 0.307785 4.2:+23917859 MS.gene39578:intron
AGAGTTCTTATGAAAGCATT+TGG 0.314655 4.2:+23917352 MS.gene39578:CDS
GAGATCATCGTTGCTGGTTT+GGG 0.333139 4.2:-23916155 None:intergenic
ACCTTTAGTAGCTGCTATAA+AGG 0.342177 4.2:-23917284 None:intergenic
CTTTCTAAACTCATTGAAAC+AGG 0.346381 4.2:-23917328 None:intergenic
TTTGTATCTTTCCATATCTC+TGG 0.353997 4.2:-23917523 None:intergenic
TGAGATCATCGTTGCTGGTT+TGG 0.382543 4.2:-23916156 None:intergenic
AGAATACTGGGCCAGAGATA+TGG 0.384193 4.2:+23917512 MS.gene39578:CDS
ACCGGTGGGAACACAGGAAT+TGG 0.391141 4.2:+23917256 MS.gene39578:CDS
AGCCCTTAGTGATACTCTTC+AGG 0.405705 4.2:-23916321 None:intergenic
ACTCATAGCCTTGGAATATT+CGG 0.408095 4.2:-23917392 None:intergenic
AGCACTCCAGGAAGGCTTGC+TGG 0.419636 4.2:+23918085 MS.gene39578:CDS
GTATTCTCATAATGCACCTT+AGG 0.423127 4.2:-23917496 None:intergenic
AATATTGAGATCATCGTTGC+TGG 0.427113 4.2:-23916161 None:intergenic
ATTTCTTTATCCAAATTCTT+GGG 0.427230 4.2:-23917902 None:intergenic
TAATGCACCTTAGGATTTGA+AGG 0.427650 4.2:-23917487 None:intergenic
CATATCTTTCACCGAAGCTT+GGG 0.435988 4.2:-23918640 None:intergenic
GTTTGGGATTCGACAAAGGC+AGG 0.443433 4.2:-23916139 None:intergenic
TTAGTCTCGGCAATTGGAAC+CGG 0.448696 4.2:+23917562 MS.gene39578:CDS
TCCGACCTCTGTAATATCAT+TGG 0.450501 4.2:-23916185 None:intergenic
TGTTGCAGCATGTATGCATT+AGG 0.450753 4.2:-23917454 None:intergenic
AGCTGAAACGGTGGCTGGTA+CGG 0.450973 4.2:-23916093 None:intergenic
AACTGCTCCACTCATAGCCT+TGG 0.451930 4.2:-23917401 None:intergenic
CAGGTTGTATTCCCAAGCTT+CGG 0.453220 4.2:+23918629 MS.gene39578:intron
GCCTTTATAGCAGCTACTAA+AGG 0.455275 4.2:+23917283 MS.gene39578:CDS
ACATATCTTTCACCGAAGCT+TGG 0.458571 4.2:-23918641 None:intergenic
CCACACATAATTCAGGGTAT+TGG 0.460806 4.2:+23917866 MS.gene39578:CDS
GTAAAATTGTTTCATGGCTC+CGG 0.463120 4.2:-23918713 None:intergenic
AAACAGGTGGGAATTTCCTC+AGG 0.469541 4.2:+23918294 MS.gene39578:intron
TGCTTACATGCACAGCTCAT+AGG 0.475577 4.2:+23916280 MS.gene39578:intron
AAGGTGCATTATGAGAATAC+TGG 0.479612 4.2:+23917499 MS.gene39578:CDS
TGATAATCCTTCAAATCCTA+AGG 0.485123 4.2:+23917480 MS.gene39578:CDS
TGCAAGGCAAATAGCACTCC+AGG 0.497957 4.2:+23918073 MS.gene39578:CDS
AGAATGTGAGAAAATGCAAC+CGG 0.512076 4.2:+23918694 MS.gene39578:CDS
AGATCCTGGTTGAGCTGAAA+CGG 0.512803 4.2:-23916105 None:intergenic
TTGGGATTCGACAAAGGCAG+GGG 0.517966 4.2:-23916137 None:intergenic
CAGAAAGGACTCATAACACC+TGG 0.522023 4.2:+23917090 MS.gene39578:CDS
ATAATTCAGGGTATTGGGGC+TGG 0.523924 4.2:+23917872 MS.gene39578:CDS
TGCTAAGAGACCTGAAAATG+AGG 0.527044 4.2:+23918344 MS.gene39578:CDS
GTCTCGGCAATTGGAACCGG+TGG 0.532547 4.2:+23917565 MS.gene39578:CDS
GAGATATGGAAAGATACAAA+AGG 0.534527 4.2:+23917526 MS.gene39578:CDS
GGTGGAACTCTTTCTGGAGC+TGG 0.534617 4.2:+23917583 MS.gene39578:CDS
CATGTTGCAGTGTCAAAGAC+AGG 0.537458 4.2:+23916383 MS.gene39578:CDS
TGTTATGAGTCCTTTCTGCT+CGG 0.547510 4.2:-23917085 None:intergenic
AGGGGAAGATGAAAAGATCC+TGG 0.553418 4.2:-23916119 None:intergenic
ACAAGTAAAAGACTTGACTA+AGG 0.556177 4.2:-23916056 None:intergenic
AGCTCCAGAAAGAGTTCCAC+CGG 0.558578 4.2:-23917581 None:intergenic
AGGTGCATTATGAGAATACT+GGG 0.564800 4.2:+23917500 MS.gene39578:CDS
ACAGGACCACACATAATTCA+GGG 0.566612 4.2:+23917860 MS.gene39578:intron
ACCAATTCCTGTGTTCCCAC+CGG 0.567362 4.2:-23917257 None:intergenic
TTACCTGAAGAGTATCACTA+AGG 0.569604 4.2:+23916318 MS.gene39578:CDS
GGTTGAGCTGAAACGGTGGC+TGG 0.574406 4.2:-23916098 None:intergenic
ATTCTTGATGCCGAGCAGAA+AGG 0.581925 4.2:+23917075 MS.gene39578:CDS
TTTGGGATTCGACAAAGGCA+GGG 0.588345 4.2:-23916138 None:intergenic
GAACCTACCGGTGGGAACAC+AGG 0.590747 4.2:+23917250 MS.gene39578:CDS
CTACGTATCAATCAGTGCAT+CGG 0.594890 4.2:+23917703 MS.gene39578:intron
ATACTCTTCAGGTAAACCAT+TGG 0.603385 4.2:-23916310 None:intergenic
AGGACTCATAACACCTGGGA+AGG 0.604876 4.2:+23917095 MS.gene39578:CDS
GAAAGTATGTTGCTTTCGAG+AGG 0.608190 4.2:-23917733 None:intergenic
ACACATAATTCAGGGTATTG+GGG 0.608497 4.2:+23917868 MS.gene39578:CDS
CTGTCTTTGACACTGCAACA+TGG 0.615946 4.2:-23916382 None:intergenic
TCCTGGTTGAGCTGAAACGG+TGG 0.618636 4.2:-23916102 None:intergenic
GCTAAGAGACCTGAAAATGA+GGG 0.619429 4.2:+23918345 MS.gene39578:CDS
TACCTGAAGAGTATCACTAA+GGG 0.627372 4.2:+23916319 MS.gene39578:CDS
GAATATTCCAAGGCTATGAG+TGG 0.632482 4.2:+23917394 MS.gene39578:CDS
GCTGGTTTGGGATTCGACAA+AGG 0.632618 4.2:-23916143 None:intergenic
AGACCGCTATAACTTCATCA+AGG 0.636275 4.2:-23917925 None:intergenic
GCCACCGTTTCAGCTCAACC+AGG 0.639745 4.2:+23916101 MS.gene39578:CDS
AAGAATCTGTCAAAACTGCA+AGG 0.645155 4.2:+23918057 MS.gene39578:CDS
ATTCCTGTGTTCCCACCGGT+AGG 0.651234 4.2:-23917253 None:intergenic
GAAAGCAACATACTTTCAGG+TGG 0.653711 4.2:+23917739 MS.gene39578:CDS
AAATGAGGGAAAGCTTATTG+TGG 0.653863 4.2:+23918359 MS.gene39578:CDS
AGCTGTAGCAGCACAACCTG+AGG 0.655976 4.2:-23918310 None:intergenic
AATCCTTGATGAAGTTATAG+CGG 0.663239 4.2:+23917922 MS.gene39578:CDS
AGGCAAATAGCACTCCAGGA+AGG 0.669487 4.2:+23918077 MS.gene39578:CDS
CCAATACCCTGAATTATGTG+TGG 0.679263 4.2:-23917866 None:intergenic
AGAAAGGACTCATAACACCT+GGG 0.699620 4.2:+23917091 MS.gene39578:CDS
TATTGCCAATGATATTACAG+AGG 0.723357 4.2:+23916180 MS.gene39578:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTATGCTTTAATATA+TGG - chr4.2:23918196-23918215 None:intergenic 10.0%
!! CTATTATTAGTATAAAAATA+TGG - chr4.2:23916704-23916723 None:intergenic 10.0%
!! TAAAAAAAATTACTTATGTA+AGG + chr4.2:23918399-23918418 MS.gene39578:intron 10.0%
!!! ATAAGTAATTTTTTTTAATG+CGG - chr4.2:23918396-23918415 None:intergenic 10.0%
!! AACTTATTATATTTCTTACT+TGG - chr4.2:23917769-23917788 None:intergenic 15.0%
!! ATAATAGTAGTAGATAATTT+CGG + chr4.2:23916717-23916736 MS.gene39578:intron 15.0%
!! ATTAAAGCATAATTAATGAA+GGG + chr4.2:23918199-23918218 MS.gene39578:intron 15.0%
!! CAATAAATAAAAAAATTGTG+AGG - chr4.2:23918609-23918628 None:intergenic 15.0%
!! TAGAATATAAACCTTAAATT+TGG + chr4.2:23916615-23916634 MS.gene39578:intron 15.0%
!! TATTAAAGCATAATTAATGA+AGG + chr4.2:23918198-23918217 MS.gene39578:intron 15.0%
!!! AATTTAAGGTTTATATTCTA+AGG - chr4.2:23916615-23916634 None:intergenic 15.0%
!!! ACAATTTTTTTATTTATTGC+AGG + chr4.2:23918610-23918629 MS.gene39578:intron 15.0%
!!! TTCTTTCTTTTTAAACATTT+TGG + chr4.2:23916439-23916458 MS.gene39578:intron 15.0%
!! ACAACAAAATCCAAAAATAA+AGG + chr4.2:23917618-23917637 MS.gene39578:CDS 20.0%
!! ATATCAGCTAATTAATTGTA+AGG - chr4.2:23916840-23916859 None:intergenic 20.0%
!! ATTTCTTTATCCAAATTCTT+GGG - chr4.2:23917905-23917924 None:intergenic 20.0%
!! GTAGAGAATAAAATGTTAAA+TGG - chr4.2:23917687-23917706 None:intergenic 20.0%
!! TTTGTTTCATTTCTATTTGT+TGG + chr4.2:23916485-23916504 MS.gene39578:intron 20.0%
!!! GAATGATTTACCTTTATTTT+TGG - chr4.2:23917631-23917650 None:intergenic 20.0%
!!! TTATTTTATTGTTTCTGCTT+TGG + chr4.2:23917026-23917045 MS.gene39578:intron 20.0%
!!! TTTTTGGATTTTGTTGTTTT+AGG - chr4.2:23917615-23917634 None:intergenic 20.0%
! ATAACAGAATATGATCAGAT+TGG - chr4.2:23918491-23918510 None:intergenic 25.0%
! ATCAGATAAAAGCTCTTTAA+AGG - chr4.2:23917821-23917840 None:intergenic 25.0%
! ATTTCGGCTAAATAATTGTA+AGG + chr4.2:23916733-23916752 MS.gene39578:intron 25.0%
! CACTTATATGCAAATTTATG+TGG - chr4.2:23916974-23916993 None:intergenic 25.0%
! GATTATTCAGAATAATGCAT+TGG - chr4.2:23917984-23918003 None:intergenic 25.0%
! GATTGTTAATTACTTGTTTC+TGG + chr4.2:23917203-23917222 MS.gene39578:intron 25.0%
! GATTTCTTTATCCAAATTCT+TGG - chr4.2:23917906-23917925 None:intergenic 25.0%
! TGGTATCAATGATGTTATAT+CGG + chr4.2:23916771-23916790 MS.gene39578:intron 25.0%
! TTGTAAGGAATAAATGTTGT+TGG + chr4.2:23916748-23916767 MS.gene39578:intron 25.0%
! TTTACCTAAATTCTTACCAT+TGG + chr4.2:23918270-23918289 MS.gene39578:intron 25.0%
! TTTGTATCTTGATTAACTGT+TGG + chr4.2:23918232-23918251 MS.gene39578:intron 25.0%
!! TTTTCCAATGGTAAGAATTT+AGG - chr4.2:23918277-23918296 None:intergenic 25.0%
!!! TCTTTTTTATATACCATCCA+AGG - chr4.2:23918440-23918459 None:intergenic 25.0%
AATCCTTGATGAAGTTATAG+CGG + chr4.2:23917922-23917941 MS.gene39578:CDS 30.0%
AATTCTTACCATTGGAAAAC+AGG + chr4.2:23918278-23918297 MS.gene39578:intron 30.0%
ACAAGTAAAAGACTTGACTA+AGG - chr4.2:23916059-23916078 None:intergenic 30.0%
AGGAAATAACATATCAGACT+TGG - chr4.2:23917801-23917820 None:intergenic 30.0%
CTTTCTAAACTCATTGAAAC+AGG - chr4.2:23917331-23917350 None:intergenic 30.0%
GAGATATGGAAAGATACAAA+AGG + chr4.2:23917526-23917545 MS.gene39578:CDS 30.0%
TAAATGGAAATCAACAAGCT+AGG - chr4.2:23917671-23917690 None:intergenic 30.0%
TAAGGAATAAATGTTGTTGG+TGG + chr4.2:23916751-23916770 MS.gene39578:intron 30.0%
TATTGCCAATGATATTACAG+AGG + chr4.2:23916180-23916199 MS.gene39578:CDS 30.0%
TGATAATCCTTCAAATCCTA+AGG + chr4.2:23917480-23917499 MS.gene39578:CDS 30.0%
TTTAATTAATCCACCTTGGA+TGG + chr4.2:23918424-23918443 MS.gene39578:intron 30.0%
TTTGTATCTTTCCATATCTC+TGG - chr4.2:23917526-23917545 None:intergenic 30.0%
! TAGTCAATGGGTGATATTTT+TGG + chr4.2:23916515-23916534 MS.gene39578:intron 30.0%
! TTTTTATATACCATCCAAGG+TGG - chr4.2:23918437-23918456 None:intergenic 30.0%
!! AGAGTTCTTATGAAAGCATT+TGG + chr4.2:23917352-23917371 MS.gene39578:CDS 30.0%
!! CAAAGTAGACATTTTAGTCT+CGG + chr4.2:23917549-23917568 MS.gene39578:CDS 30.0%
!!! ATTTTTTCCTGCTAAATCAC+AGG + chr4.2:23917842-23917861 MS.gene39578:intron 30.0%
AAATGAGGGAAAGCTTATTG+TGG + chr4.2:23918359-23918378 MS.gene39578:CDS 35.0%
AAGAATCTGTCAAAACTGCA+AGG + chr4.2:23918057-23918076 MS.gene39578:CDS 35.0%
AAGGTGCATTATGAGAATAC+TGG + chr4.2:23917499-23917518 MS.gene39578:CDS 35.0%
AATATTGAGATCATCGTTGC+TGG - chr4.2:23916164-23916183 None:intergenic 35.0%
ACCTTTAGTAGCTGCTATAA+AGG - chr4.2:23917287-23917306 None:intergenic 35.0%
AGAATGTGAGAAAATGCAAC+CGG + chr4.2:23918694-23918713 MS.gene39578:CDS 35.0%
AGGTGCATTATGAGAATACT+GGG + chr4.2:23917500-23917519 MS.gene39578:CDS 35.0%
ATACTCTTCAGGTAAACCAT+TGG - chr4.2:23916313-23916332 None:intergenic 35.0%
GATAAGACGGTCCAAATTTA+AGG - chr4.2:23916629-23916648 None:intergenic 35.0%
GGAGTTTAATTAATCCACCT+TGG + chr4.2:23918420-23918439 MS.gene39578:intron 35.0%
GTATTCTCATAATGCACCTT+AGG - chr4.2:23917499-23917518 None:intergenic 35.0%
GTTGGTTAGTGTTAGTCAAT+GGG + chr4.2:23916503-23916522 MS.gene39578:intron 35.0%
TAATGCACCTTAGGATTTGA+AGG - chr4.2:23917490-23917509 None:intergenic 35.0%
TTTCTTGCAATTGACAACAC+TGG + chr4.2:23916931-23916950 MS.gene39578:intron 35.0%
! AATATGATCAGATTGGCTGA+AGG - chr4.2:23918484-23918503 None:intergenic 35.0%
! ACTCATAGCCTTGGAATATT+CGG - chr4.2:23917395-23917414 None:intergenic 35.0%
! ATAAGCTTTCCCTCATTTTC+AGG - chr4.2:23918357-23918376 None:intergenic 35.0%
! ATATGATCAGATTGGCTGAA+GGG - chr4.2:23918483-23918502 None:intergenic 35.0%
! CACACATAATTCAGGGTATT+GGG + chr4.2:23917867-23917886 MS.gene39578:CDS 35.0%
! TGTTGGTTAGTGTTAGTCAA+TGG + chr4.2:23916502-23916521 MS.gene39578:intron 35.0%
!! ACACATAATTCAGGGTATTG+GGG + chr4.2:23917868-23917887 MS.gene39578:CDS 35.0%
!! GTATGCACTTCAGAGTATTT+TGG + chr4.2:23917225-23917244 MS.gene39578:intron 35.0%
!! TACCTGAAGAGTATCACTAA+GGG + chr4.2:23916319-23916338 MS.gene39578:CDS 35.0%
!! TTACCTGAAGAGTATCACTA+AGG + chr4.2:23916318-23916337 MS.gene39578:CDS 35.0%
!!! AGTATTTTGGTAGAACCTAC+CGG + chr4.2:23917238-23917257 MS.gene39578:CDS 35.0%
!!! TGTTTTGACCGAATATTCCA+AGG + chr4.2:23917384-23917403 MS.gene39578:CDS 35.0%
ACAGGACCACACATAATTCA+GGG + chr4.2:23917860-23917879 MS.gene39578:intron 40.0%
ACATATCTTTCACCGAAGCT+TGG - chr4.2:23918644-23918663 None:intergenic 40.0%
ACCGTCTGGTTAAGATAAGA+CGG - chr4.2:23916642-23916661 None:intergenic 40.0%
ACCGTCTTATCTTAACCAGA+CGG + chr4.2:23916638-23916657 MS.gene39578:intron 40.0%
AGAAAGGACTCATAACACCT+GGG + chr4.2:23917091-23917110 MS.gene39578:CDS 40.0%
AGACCGCTATAACTTCATCA+AGG - chr4.2:23917928-23917947 None:intergenic 40.0%
ATGCTATAACCTATCCTGCA+AGG - chr4.2:23917054-23917073 None:intergenic 40.0%
CATATCTTTCACCGAAGCTT+GGG - chr4.2:23918643-23918662 None:intergenic 40.0%
CCAATACCCTGAATTATGTG+TGG - chr4.2:23917869-23917888 None:intergenic 40.0%
CCACACATAATTCAGGGTAT+TGG + chr4.2:23917866-23917885 MS.gene39578:CDS 40.0%
CTACGTATCAATCAGTGCAT+CGG + chr4.2:23917703-23917722 MS.gene39578:intron 40.0%
CTCGAAAGCAACATACTTTC+AGG + chr4.2:23917736-23917755 MS.gene39578:CDS 40.0%
CTTACCATTGGAAAACAGGT+GGG + chr4.2:23918282-23918301 MS.gene39578:intron 40.0%
GAAAGCAACATACTTTCAGG+TGG + chr4.2:23917739-23917758 MS.gene39578:CDS 40.0%
GAATATTCCAAGGCTATGAG+TGG + chr4.2:23917394-23917413 MS.gene39578:CDS 40.0%
GCCAATGATATTACAGAGGT+CGG + chr4.2:23916184-23916203 MS.gene39578:intron 40.0%
GCCTTTATAGCAGCTACTAA+AGG + chr4.2:23917283-23917302 MS.gene39578:CDS 40.0%
GCTAAGAGACCTGAAAATGA+GGG + chr4.2:23918345-23918364 MS.gene39578:CDS 40.0%
GCTATAGCGTTATAGCATAG+CGG + chr4.2:23916793-23916812 MS.gene39578:intron 40.0%
TACGCTACAATGCTAAGCAA+TGG - chr4.2:23916867-23916886 None:intergenic 40.0%
TCCGACCTCTGTAATATCAT+TGG - chr4.2:23916188-23916207 None:intergenic 40.0%
TCTTACCATTGGAAAACAGG+TGG + chr4.2:23918281-23918300 MS.gene39578:intron 40.0%
TGCTAAGAGACCTGAAAATG+AGG + chr4.2:23918344-23918363 MS.gene39578:CDS 40.0%
TGTTATGAGTCCTTTCTGCT+CGG - chr4.2:23917088-23917107 None:intergenic 40.0%
TGTTGCAGCATGTATGCATT+AGG - chr4.2:23917457-23917476 None:intergenic 40.0%
TTGCAATTGACAACACTGGT+TGG + chr4.2:23916935-23916954 MS.gene39578:intron 40.0%
! AATTCCCACCTGTTTTCCAA+TGG - chr4.2:23918289-23918308 None:intergenic 40.0%
! CTTTCGTATTTTACCTTCCC+AGG - chr4.2:23917111-23917130 None:intergenic 40.0%
! CTTTTCTGATTGTCTGCATG+TGG + chr4.2:23918012-23918031 MS.gene39578:intron 40.0%
! TGGCAACTTTTGCAGATATC+CGG + chr4.2:23918032-23918051 MS.gene39578:intron 40.0%
! TGTGTCTACTTCTCTTTTCC+AGG + chr4.2:23918661-23918680 MS.gene39578:CDS 40.0%
!! AGTTTTGACAGATTCTTCGC+CGG - chr4.2:23918054-23918073 None:intergenic 40.0%
!! GAAAGTATGTTGCTTTCGAG+AGG - chr4.2:23917736-23917755 None:intergenic 40.0%
!! GACATTTTAGTCTCGGCAAT+TGG + chr4.2:23917556-23917575 MS.gene39578:CDS 40.0%
!!! TGGTTTTGTGCCCAAGAATT+TGG + chr4.2:23917892-23917911 MS.gene39578:CDS 40.0%
AAACAGGTGGGAATTTCCTC+AGG + chr4.2:23918294-23918313 MS.gene39578:intron 45.0%
AGAATACTGGGCCAGAGATA+TGG + chr4.2:23917512-23917531 MS.gene39578:CDS 45.0%
AGATCCTGGTTGAGCTGAAA+CGG - chr4.2:23916108-23916127 None:intergenic 45.0%
AGCCCTTAGTGATACTCTTC+AGG - chr4.2:23916324-23916343 None:intergenic 45.0%
AGGGGAAGATGAAAAGATCC+TGG - chr4.2:23916122-23916141 None:intergenic 45.0%
ATTCTTGATGCCGAGCAGAA+AGG + chr4.2:23917075-23917094 MS.gene39578:CDS 45.0%
CACAGGACCACACATAATTC+AGG + chr4.2:23917859-23917878 MS.gene39578:intron 45.0%
CAGAAAGGACTCATAACACC+TGG + chr4.2:23917090-23917109 MS.gene39578:CDS 45.0%
CAGGTTGTATTCCCAAGCTT+CGG + chr4.2:23918629-23918648 MS.gene39578:intron 45.0%
CATGTTGCAGTGTCAAAGAC+AGG + chr4.2:23916383-23916402 MS.gene39578:CDS 45.0%
CTGTCTTTGACACTGCAACA+TGG - chr4.2:23916385-23916404 None:intergenic 45.0%
GCTCATAGGAAAAACGCCAA+TGG + chr4.2:23916294-23916313 MS.gene39578:intron 45.0%
TATGCATGACTGCATGTCCT+CGG - chr4.2:23916544-23916563 None:intergenic 45.0%
TGCTGCTAAGCTTGAGCTTA+TGG + chr4.2:23916357-23916376 MS.gene39578:CDS 45.0%
TGCTTACATGCACAGCTCAT+AGG + chr4.2:23916280-23916299 MS.gene39578:intron 45.0%
TTAGTCTCGGCAATTGGAAC+CGG + chr4.2:23917562-23917581 MS.gene39578:CDS 45.0%
TTGCAGTGTCAAAGACAGGT+AGG + chr4.2:23916387-23916406 MS.gene39578:intron 45.0%
! TGAGATCATCGTTGCTGGTT+TGG - chr4.2:23916159-23916178 None:intergenic 45.0%
! TTTCTGCTTTGGTTCCTTGC+AGG + chr4.2:23917037-23917056 MS.gene39578:intron 45.0%
! TTTGGGATTCGACAAAGGCA+GGG - chr4.2:23916141-23916160 None:intergenic 45.0%
! TTTTGGTAGAACCTACCGGT+GGG + chr4.2:23917242-23917261 MS.gene39578:CDS 45.0%
!! ATAATTCAGGGTATTGGGGC+TGG + chr4.2:23917872-23917891 MS.gene39578:CDS 45.0%
!! ATTTTGGTAGAACCTACCGG+TGG + chr4.2:23917241-23917260 MS.gene39578:CDS 45.0%
!! GAGATCATCGTTGCTGGTTT+GGG - chr4.2:23916158-23916177 None:intergenic 45.0%
!!! GGTGATATTTTTGGATCCCG+AGG + chr4.2:23916524-23916543 MS.gene39578:intron 45.0%
!! TATTATTAGTATAAAAATAT+GGG - chr4.2:23916703-23916722 None:intergenic 5.0%
AACTGCTCCACTCATAGCCT+TGG - chr4.2:23917404-23917423 None:intergenic 50.0%
ACCAATTCCTGTGTTCCCAC+CGG - chr4.2:23917260-23917279 None:intergenic 50.0%
AGCTCCAGAAAGAGTTCCAC+CGG - chr4.2:23917584-23917603 None:intergenic 50.0%
AGGACTCATAACACCTGGGA+AGG + chr4.2:23917095-23917114 MS.gene39578:CDS 50.0%
AGGCAAATAGCACTCCAGGA+AGG + chr4.2:23918077-23918096 MS.gene39578:CDS 50.0%
ATGCATGACTGCATGTCCTC+GGG - chr4.2:23916543-23916562 None:intergenic 50.0%
CACATTCTTCTCGAGCTTCC+TGG - chr4.2:23918682-23918701 None:intergenic 50.0%
GTGTGGTCCTGTGATTTAGC+AGG - chr4.2:23917852-23917871 None:intergenic 50.0%
TGCAAGGCAAATAGCACTCC+AGG + chr4.2:23918073-23918092 MS.gene39578:CDS 50.0%
TTGGGATTCGACAAAGGCAG+GGG - chr4.2:23916140-23916159 None:intergenic 50.0%
! GCTTTGGTTCCTTGCAGGAT+AGG + chr4.2:23917042-23917061 MS.gene39578:intron 50.0%
! GTTTGGGATTCGACAAAGGC+AGG - chr4.2:23916142-23916161 None:intergenic 50.0%
!! GCTGGTTTGGGATTCGACAA+AGG - chr4.2:23916146-23916165 None:intergenic 50.0%
ACCGGTGGGAACACAGGAAT+TGG + chr4.2:23917256-23917275 MS.gene39578:CDS 55.0%
AGCTGAAACGGTGGCTGGTA+CGG - chr4.2:23916096-23916115 None:intergenic 55.0%
AGCTGTAGCAGCACAACCTG+AGG - chr4.2:23918313-23918332 None:intergenic 55.0%
ATGCATGCAGTCCTGCCTTC+TGG - chr4.2:23916674-23916693 None:intergenic 55.0%
ATTCCTGTGTTCCCACCGGT+AGG - chr4.2:23917256-23917275 None:intergenic 55.0%
CACTAACCAGCAAGCCTTCC+TGG - chr4.2:23918094-23918113 None:intergenic 55.0%
GACGGTGAGATCTGTCCAGA+AGG + chr4.2:23916656-23916675 MS.gene39578:intron 55.0%
GGAACCGGTGGAACTCTTTC+TGG + chr4.2:23917577-23917596 MS.gene39578:CDS 55.0%
GTGAGATCTGTCCAGAAGGC+AGG + chr4.2:23916660-23916679 MS.gene39578:intron 55.0%
TCCTGGTTGAGCTGAAACGG+TGG - chr4.2:23916105-23916124 None:intergenic 55.0%
TCTGGACAGATCTCACCGTC+TGG - chr4.2:23916656-23916675 None:intergenic 55.0%
TGCTGCTACAGCTGCATTGC+AGG + chr4.2:23918320-23918339 MS.gene39578:CDS 55.0%
!! GGTGGAACTCTTTCTGGAGC+TGG + chr4.2:23917583-23917602 MS.gene39578:CDS 55.0%
AGCACTCCAGGAAGGCTTGC+TGG + chr4.2:23918085-23918104 MS.gene39578:CDS 60.0%
GAACCTACCGGTGGGAACAC+AGG + chr4.2:23917250-23917269 MS.gene39578:CDS 60.0%
GCCACCGTTTCAGCTCAACC+AGG + chr4.2:23916101-23916120 MS.gene39578:CDS 60.0%
GGTTGAGCTGAAACGGTGGC+TGG - chr4.2:23916101-23916120 None:intergenic 60.0%
GTCTCGGCAATTGGAACCGG+TGG + chr4.2:23917565-23917584 MS.gene39578:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 23916043 23918724 23916043 ID=MS.gene39578
chr4.2 mRNA 23916043 23918724 23916043 ID=MS.gene39578.t1;Parent=MS.gene39578
chr4.2 exon 23916043 23916201 23916043 ID=MS.gene39578.t1.exon1;Parent=MS.gene39578.t1
chr4.2 CDS 23916043 23916201 23916043 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23916295 23916404 23916295 ID=MS.gene39578.t1.exon2;Parent=MS.gene39578.t1
chr4.2 CDS 23916295 23916404 23916295 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23917059 23917116 23917059 ID=MS.gene39578.t1.exon3;Parent=MS.gene39578.t1
chr4.2 CDS 23917059 23917116 23917059 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23917238 23917639 23917238 ID=MS.gene39578.t1.exon4;Parent=MS.gene39578.t1
chr4.2 CDS 23917238 23917639 23917238 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23917718 23917769 23917718 ID=MS.gene39578.t1.exon5;Parent=MS.gene39578.t1
chr4.2 CDS 23917718 23917769 23917718 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23917864 23917943 23917864 ID=MS.gene39578.t1.exon6;Parent=MS.gene39578.t1
chr4.2 CDS 23917864 23917943 23917864 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23918047 23918106 23918047 ID=MS.gene39578.t1.exon7;Parent=MS.gene39578.t1
chr4.2 CDS 23918047 23918106 23918047 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23918300 23918380 23918300 ID=MS.gene39578.t1.exon8;Parent=MS.gene39578.t1
chr4.2 CDS 23918300 23918380 23918300 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
chr4.2 exon 23918632 23918724 23918632 ID=MS.gene39578.t1.exon9;Parent=MS.gene39578.t1
chr4.2 CDS 23918632 23918724 23918632 ID=cds.MS.gene39578.t1;Parent=MS.gene39578.t1
Gene Sequence

>MS.gene39578

ATGGCTTCTTCTTCCTTAGTCAAGTCTTTTACTTGTGCTTTGCGTGCTCGCCGTACCAGCCACCGTTTCAGCTCAACCAGGATCTTTTCATCTTCCCCTGCCTTTGTCGAATCCCAAACCAGCAACGATGATCTCAATATTGCCAATGATATTACAGAGGTCGGATTCATACTATTTTTGATTTGTTTTTTCTTATTTCATAGTATGCAATTCAATGATGAATCTCGTTTTTTTTGCTGCTTACATGCACAGCTCATAGGAAAAACGCCAATGGTTTACCTGAAGAGTATCACTAAGGGCTCAGTTGCTACTATTGCTGCTAAGCTTGAGCTTATGGAGCCATGTTGCAGTGTCAAAGACAGGTAGGCGACGTGTGTGACAGCATTAGTCGTTCTCTTCTTTCTTTTTAAACATTTTGGTTTCTTTATCTTGCATTTTTGTTTTTGTTTCATTTCTATTTGTTGGTTAGTGTTAGTCAATGGGTGATATTTTTGGATCCCGAGGACATGCAGTCATGCATAGCTCTAGTTTTTCGATTAAGATCGATCAATCTTAAAGTTCACATTAAACCTTAGAATATAAACCTTAAATTTGGACCGTCTTATCTTAACCAGACGGTGAGATCTGTCCAGAAGGCAGGACTGCATGCATCTAAATCCCATATTTTTATACTAATAATAGTAGTAGATAATTTCGGCTAAATAATTGTAAGGAATAAATGTTGTTGGTGGTATCAATGATGTTATATCGGCTATAGCGTTATAGCATAGCGGTTACTGTCGAACTTAAATGTTCCTTACAATTAATTAGCTGATATAGCACCATTGCTTAGCATTGTAGCGTAGCGTTATAGCATCATTACTCAGCATTATTTGAACAAACTGTTATTTTCTTGCAATTGACAACACTGGTTGGTATTAGTATATGTCCACATAAATTTGCATATAAGTGTTCAATTCAATCTATCTCTATTTTATTTTATTTTATTTTATTGTTTCTGCTTTGGTTCCTTGCAGGATAGGTTATAGCATGATTCTTGATGCCGAGCAGAAAGGACTCATAACACCTGGGAAGGTAAAATACGAAAGAATGACTAATCATGTGCAGTGTTAATTACTTGTGCATGAGTATTAAAACAAAAAAACAATGTTTTTTCTCATGATTGTTAATTACTTGTTTCTGGTATGCACTTCAGAGTATTTTGGTAGAACCTACCGGTGGGAACACAGGAATTGGTCTTGCCTTTATAGCAGCTACTAAAGGTTATAAACTCATTTTGACAATGCCTGTTTCAATGAGTTTAGAAAGAAGAGTTCTTATGAAAGCATTTGGAGCTGAGCTTGTTTTGACCGAATATTCCAAGGCTATGAGTGGAGCAGTTCAAAAAGCTGAAGAAATTGTAAAAAATACACCTAATGCATACATGCTGCAACAATTTGATAATCCTTCAAATCCTAAGGTGCATTATGAGAATACTGGGCCAGAGATATGGAAAGATACAAAAGGCAAAGTAGACATTTTAGTCTCGGCAATTGGAACCGGTGGAACTCTTTCTGGAGCTGGTAGATTCCTAAAACAACAAAATCCAAAAATAAAGGTAAATCATTCTCAAGAGAAGCTTTCTACCTAGCTTGTTGATTTCCATTTAACATTTTATTCTCTACGTATCAATCAGTGCATCGGTGTAGAGCCTCTCGAAAGCAACATACTTTCAGGTGGAAAGCCAAGTAAGAAATATAATAAGTTTCTACAATTCCAAGTCTGATATGTTATTTCCTTTAAAGAGCTTTTATCTGATTATTTTTTCCTGCTAAATCACAGGACCACACATAATTCAGGGTATTGGGGCTGGTTTTGTGCCCAAGAATTTGGATAAAGAAATCCTTGATGAAGTTATAGCGGTCTGTTCTTGTCTCATTTTTATGTGATCATCAAAATCCAATGCATTATTCTGAATAATCAAGACATTCTTTTCTGATTGTCTGCATGTGGCAACTTTTGCAGATATCCGGCGAAGAATCTGTCAAAACTGCAAGGCAAATAGCACTCCAGGAAGGCTTGCTGGTTAGTGCTTTATGCTCATCTTTTTAGCTATCATGTGCACTTACGCAGTTACATGCTAAAGCAGCTTGAAAAAATCATGCACAAAACCATATATTAAAGCATAATTAATGAAGGGCATAAAAACTTTTGTATCTTGATTAACTGTTGGCATTATACTTTGATCTTTACCTAAATTCTTACCATTGGAAAACAGGTGGGAATTTCCTCAGGTTGTGCTGCTACAGCTGCATTGCAGGTTGCTAAGAGACCTGAAAATGAGGGAAAGCTTATTGTGGTATGCATTTATCCGCATTAAAAAAAATTACTTATGTAAGGAGTTTAATTAATCCACCTTGGATGGTATATAAAAAAGATAGAGATGTATTATATGTCTCCCTTCAGCCAATCTGATCATATTCTGTTATTTTCATAATAATGTCAACTTAATTATCTTTTACTCTTAAAAGTTTCATAAAATTTCAAATTTGCAGAATTAAAATACTTTATTTTCACTTTAATACCTCACAATTTTTTTATTTATTGCAGGTTGTATTCCCAAGCTTCGGTGAAAGATATGTGTCTACTTCTCTTTTCCAGGAAGCTCGAGAAGAATGTGAGAAAATGCAACCGGAGCCATGA
Protein sequence

>MS.gene39578.t1

MASSSLVKSFTCALRARRTSHRFSSTRIFSSSPAFVESQTSNDDLNIANDITELIGKTPMVYLKSITKGSVATIAAKLELMEPCCSVKDRIGYSMILDAEQKGLITPGKSILVEPTGGNTGIGLAFIAATKGYKLILTMPVSMSLERRVLMKAFGAELVLTEYSKAMSGAVQKAEEIVKNTPNAYMLQQFDNPSNPKVHYENTGPEIWKDTKGKVDILVSAIGTGGTLSGAGRFLKQQNPKIKCIGVEPLESNILSGGKPRPHIIQGIGAGFVPKNLDKEILDEVIAISGEESVKTARQIALQEGLLVGISSGCAATAALQVAKRPENEGKLIVVVFPSFGERYVSTSLFQEAREECEKMQPEP