Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39845.t1 | XP_003617057.2 | 95.7 | 254 | 11 | 0 | 1 | 254 | 1 | 254 | 5.70E-89 | 337.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39845.t1 | D9UC01 | 34.1 | 88 | 57 | 1 | 14 | 100 | 16 | 103 | 1.0e-09 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39845.t1 | G7KHG4 | 95.7 | 254 | 11 | 0 | 1 | 254 | 1 | 254 | 4.1e-89 | 337.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049698 | MS.gene39845 | 0.829483 | 5.02E-55 | -1.69E-46 |
| MS.gene055144 | MS.gene39845 | 0.805172 | 1.52E-49 | -1.69E-46 |
| MS.gene058351 | MS.gene39845 | 0.801955 | 7.05E-49 | -1.69E-46 |
| MS.gene058909 | MS.gene39845 | 0.811566 | 6.57E-51 | -1.69E-46 |
| MS.gene058912 | MS.gene39845 | 0.805622 | 1.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39845.t1 | MTR_5g087450 | 96.457 | 254 | 9 | 0 | 1 | 254 | 1 | 254 | 0.0 | 503 |
| MS.gene39845.t1 | MTR_5g086020 | 92.126 | 127 | 10 | 0 | 62 | 188 | 1 | 127 | 8.31e-71 | 213 |
| MS.gene39845.t1 | MTR_5g087470 | 43.605 | 172 | 84 | 5 | 3 | 169 | 1 | 164 | 3.72e-35 | 127 |
| MS.gene39845.t1 | MTR_5g087480 | 37.113 | 194 | 89 | 6 | 3 | 176 | 1 | 181 | 1.02e-27 | 108 |
| MS.gene39845.t1 | MTR_5g087480 | 44.444 | 126 | 62 | 3 | 3 | 127 | 1 | 119 | 7.02e-27 | 106 |
| MS.gene39845.t1 | MTR_7g086130 | 40.860 | 93 | 55 | 0 | 11 | 103 | 8 | 100 | 8.17e-20 | 84.7 |
| MS.gene39845.t1 | MTR_6g478190 | 40.659 | 91 | 54 | 0 | 10 | 100 | 10 | 100 | 2.21e-18 | 80.1 |
| MS.gene39845.t1 | MTR_7g086130 | 38.776 | 98 | 55 | 1 | 11 | 103 | 8 | 105 | 1.58e-17 | 79.0 |
| MS.gene39845.t1 | MTR_4g107860 | 27.234 | 235 | 156 | 5 | 13 | 237 | 21 | 250 | 4.27e-16 | 75.9 |
| MS.gene39845.t1 | MTR_8g046245 | 36.667 | 90 | 56 | 1 | 12 | 100 | 22 | 111 | 7.11e-15 | 73.9 |
| MS.gene39845.t1 | MTR_1g076910 | 34.454 | 119 | 63 | 4 | 20 | 125 | 77 | 193 | 7.98e-13 | 65.9 |
| MS.gene39845.t1 | MTR_3g007660 | 30.328 | 122 | 74 | 3 | 13 | 123 | 19 | 140 | 3.74e-12 | 65.9 |
| MS.gene39845.t1 | MTR_1g076880 | 32.174 | 115 | 65 | 3 | 22 | 125 | 78 | 190 | 1.10e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39845.t1 | AT2G27140 | 38.764 | 178 | 93 | 4 | 10 | 174 | 10 | 184 | 8.85e-32 | 117 |
| MS.gene39845.t1 | AT5G20970 | 34.538 | 249 | 133 | 6 | 11 | 243 | 7 | 241 | 2.50e-28 | 108 |
| MS.gene39845.t1 | AT1G54400 | 38.462 | 91 | 55 | 1 | 13 | 102 | 12 | 102 | 5.26e-13 | 65.9 |
| MS.gene39845.t1 | AT5G04890 | 34.091 | 88 | 57 | 1 | 14 | 100 | 16 | 103 | 9.13e-13 | 67.4 |
| MS.gene39845.t1 | AT4G10250 | 37.363 | 91 | 46 | 2 | 22 | 101 | 74 | 164 | 6.44e-12 | 63.2 |
Find 40 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGATTCCCGCTTCAAATT+TGG | 0.203921 | 5.4:-68752814 | None:intergenic |
| ACTTCTTCAATTTCTTTGTT+TGG | 0.273292 | 5.4:-68753005 | None:intergenic |
| GGTTTCTGAGGAATTGTTGC+TGG | 0.343124 | 5.4:-68752930 | None:intergenic |
| AAATTGAAGAAGTGTCTAAA+AGG | 0.356274 | 5.4:+68753015 | MS.gene39845:CDS |
| GAAGAAGCTGGTTAATTTGT+TGG | 0.370154 | 5.4:+68753205 | MS.gene39845:CDS |
| ACACTTATTCTTATGCTTCC+AGG | 0.372973 | 5.4:+68752431 | MS.gene39845:CDS |
| GTTTGGGAAGCTTAACATAT+AGG | 0.376009 | 5.4:-68752833 | None:intergenic |
| AAAGTCATTGACTATGGAAA+TGG | 0.386319 | 5.4:+68753118 | MS.gene39845:CDS |
| AATTTGAACCACCCTCTGAT+TGG | 0.401334 | 5.4:+68752387 | MS.gene39845:CDS |
| AATTAATCAATTACAACTCT+TGG | 0.411666 | 5.4:-68753307 | None:intergenic |
| GAAACTTGAGGAGCAGTGTT+TGG | 0.417491 | 5.4:-68752951 | None:intergenic |
| ATTTGAACCACCCTCTGATT+GGG | 0.445092 | 5.4:+68752388 | MS.gene39845:CDS |
| ACCCGTGTGCTAAGGGTTAG+TGG | 0.463763 | 5.4:+68752705 | MS.gene39845:CDS |
| AACCCTTAGCACACGGGTTG+AGG | 0.474881 | 5.4:-68752700 | None:intergenic |
| GTGTCTAAAAGGAAGGGAAA+AGG | 0.475085 | 5.4:+68753026 | MS.gene39845:CDS |
| TGAAGAAGTGTCTAAAAGGA+AGG | 0.482631 | 5.4:+68753019 | MS.gene39845:CDS |
| TGTGAATGCATTATGTGGTT+TGG | 0.495211 | 5.4:+68753163 | MS.gene39845:CDS |
| TAACCTCAACCCGTGTGCTA+AGG | 0.497781 | 5.4:+68752697 | MS.gene39845:CDS |
| CATTCTGACACTAGCAGCAT+TGG | 0.497948 | 5.4:+68752789 | MS.gene39845:CDS |
| CACCACTAACCCTTAGCACA+CGG | 0.507093 | 5.4:-68752707 | None:intergenic |
| ATTTAAGAAGGATCAATTGA+GGG | 0.509297 | 5.4:+68752668 | MS.gene39845:CDS |
| GGAATTGTTGCTGGTTGTTG+AGG | 0.516048 | 5.4:-68752921 | None:intergenic |
| GAATGCATTATGTGGTTTGG+TGG | 0.533238 | 5.4:+68753166 | MS.gene39845:CDS |
| GATTTAAGAAGGATCAATTG+AGG | 0.534085 | 5.4:+68752667 | MS.gene39845:CDS |
| AATACATGCAGGATTTAAGA+AGG | 0.538421 | 5.4:+68752656 | MS.gene39845:intron |
| AACCTCAACCCGTGTGCTAA+GGG | 0.546287 | 5.4:+68752698 | MS.gene39845:CDS |
| AGTTAAGAAGCAGAAGAAGC+TGG | 0.549223 | 5.4:+68753193 | MS.gene39845:CDS |
| ATTGGTGCCAAATTTGAAGC+GGG | 0.549918 | 5.4:+68752807 | MS.gene39845:CDS |
| GGAGCAGTGTTTGGTTTCTG+AGG | 0.565721 | 5.4:-68752942 | None:intergenic |
| ACCACTAACCCTTAGCACAC+GGG | 0.576619 | 5.4:-68752706 | None:intergenic |
| GAATATGTGAATGCATTATG+TGG | 0.588342 | 5.4:+68753158 | MS.gene39845:CDS |
| GAAGAAGTGTCTAAAAGGAA+GGG | 0.593904 | 5.4:+68753020 | MS.gene39845:CDS |
| CATTGGTGCCAAATTTGAAG+CGG | 0.594150 | 5.4:+68752806 | MS.gene39845:CDS |
| ACCCTCTGATTGGGATCATG+AGG | 0.602046 | 5.4:+68752397 | MS.gene39845:CDS |
| TCCTCATGATCCCAATCAGA+GGG | 0.610175 | 5.4:-68752398 | None:intergenic |
| GAAGGGAAAAGGTAAAGCTG+AGG | 0.645097 | 5.4:+68753037 | MS.gene39845:CDS |
| ATCCTCATGATCCCAATCAG+AGG | 0.659662 | 5.4:-68752399 | None:intergenic |
| ATGCTGCTAGTGTCAGAATG+AGG | 0.675243 | 5.4:-68752786 | None:intergenic |
| TCATGATCCCAATCAGAGGG+TGG | 0.690466 | 5.4:-68752395 | None:intergenic |
| CTGCTAGTGTCAGAATGAGG+TGG | 0.722333 | 5.4:-68752783 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAATTAATTCATTTTTTTC+AGG | + | chr5.4:68753272-68753291 | MS.gene39845:CDS | 15.0% |
| !!! | TTTCTTTTGATAAAACTTAA+AGG | + | chr5.4:68752558-68752577 | MS.gene39845:intron | 15.0% |
| !!! | ATTAATTCATTTTTTTCAGG+AGG | + | chr5.4:68753275-68753294 | MS.gene39845:CDS | 20.0% |
| ! | ACTTCTTCAATTTCTTTGTT+TGG | - | chr5.4:68753008-68753027 | None:intergenic | 25.0% |
| ! | ATTTAAGAAGGATCAATTGA+GGG | + | chr5.4:68752668-68752687 | MS.gene39845:CDS | 25.0% |
| ! | GACAAATATATAAGTATACG+AGG | + | chr5.4:68752582-68752601 | MS.gene39845:intron | 25.0% |
| ! | GACAAATGAATGAGAAAAAA+TGG | + | chr5.4:68752733-68752752 | MS.gene39845:CDS | 25.0% |
| ! | TATTATATGAAAAAGACACG+AGG | - | chr5.4:68752475-68752494 | None:intergenic | 25.0% |
| !! | TGAAAAAAAGTCATTGACTA+TGG | + | chr5.4:68753112-68753131 | MS.gene39845:CDS | 25.0% |
| !!! | AAATTGAAGAAGTGTCTAAA+AGG | + | chr5.4:68753015-68753034 | MS.gene39845:CDS | 25.0% |
| AAGAAAGAAATGAACTAACC+TGG | - | chr5.4:68752452-68752471 | None:intergenic | 30.0% | |
| AATACATGCAGGATTTAAGA+AGG | + | chr5.4:68752656-68752675 | MS.gene39845:intron | 30.0% | |
| GAATATGTGAATGCATTATG+TGG | + | chr5.4:68753158-68753177 | MS.gene39845:CDS | 30.0% | |
| GATTTAAGAAGGATCAATTG+AGG | + | chr5.4:68752667-68752686 | MS.gene39845:CDS | 30.0% | |
| ! | AAAGTCATTGACTATGGAAA+TGG | + | chr5.4:68753118-68753137 | MS.gene39845:CDS | 30.0% |
| ! | GTGTTGGAACAATATTTTGT+TGG | - | chr5.4:68752866-68752885 | None:intergenic | 30.0% |
| !!! | ATTTTGTTGGTTGATGAGTT+TGG | - | chr5.4:68752853-68752872 | None:intergenic | 30.0% |
| !!! | GATGTACTACTTGTTTTTTC+TGG | - | chr5.4:68752980-68752999 | None:intergenic | 30.0% |
| !!! | TGTTGTGTTGTTGATTTTGT+TGG | - | chr5.4:68752888-68752907 | None:intergenic | 30.0% |
| !!! | TTTTGTTGGTTGATGAGTTT+GGG | - | chr5.4:68752852-68752871 | None:intergenic | 30.0% |
| ACACTTATTCTTATGCTTCC+AGG | + | chr5.4:68752431-68752450 | MS.gene39845:CDS | 35.0% | |
| CTATGATCGATAATACATGC+AGG | + | chr5.4:68752645-68752664 | MS.gene39845:intron | 35.0% | |
| GTTTGGGAAGCTTAACATAT+AGG | - | chr5.4:68752836-68752855 | None:intergenic | 35.0% | |
| TGTGAATGCATTATGTGGTT+TGG | + | chr5.4:68753163-68753182 | MS.gene39845:CDS | 35.0% | |
| !! | GAAGAAGCTGGTTAATTTGT+TGG | + | chr5.4:68753205-68753224 | MS.gene39845:CDS | 35.0% |
| !! | GAAGAAGTGTCTAAAAGGAA+GGG | + | chr5.4:68753020-68753039 | MS.gene39845:CDS | 35.0% |
| !! | TGAAGAAGTGTCTAAAAGGA+AGG | + | chr5.4:68753019-68753038 | MS.gene39845:CDS | 35.0% |
| !! | TTTGTTGGTGCTTGTGTTTT+TGG | + | chr5.4:68753220-68753239 | MS.gene39845:CDS | 35.0% |
| !! | TTTTTCTGGTCAGAAACTTG+AGG | - | chr5.4:68752966-68752985 | None:intergenic | 35.0% |
| !!! | GTTGTTGATTTTGTTGGTGT+TGG | - | chr5.4:68752882-68752901 | None:intergenic | 35.0% |
| AATTTGAACCACCCTCTGAT+TGG | + | chr5.4:68752387-68752406 | MS.gene39845:CDS | 40.0% | |
| AGTTAAGAAGCAGAAGAAGC+TGG | + | chr5.4:68753193-68753212 | MS.gene39845:CDS | 40.0% | |
| ATTTGAACCACCCTCTGATT+GGG | + | chr5.4:68752388-68752407 | MS.gene39845:CDS | 40.0% | |
| GTGTCTAAAAGGAAGGGAAA+AGG | + | chr5.4:68753026-68753045 | MS.gene39845:CDS | 40.0% | |
| TAGGATTCCCGCTTCAAATT+TGG | - | chr5.4:68752817-68752836 | None:intergenic | 40.0% | |
| ! | GAATGCATTATGTGGTTTGG+TGG | + | chr5.4:68753166-68753185 | MS.gene39845:CDS | 40.0% |
| !! | ATTGGTGCCAAATTTGAAGC+GGG | + | chr5.4:68752807-68752826 | MS.gene39845:CDS | 40.0% |
| !! | CATTGGTGCCAAATTTGAAG+CGG | + | chr5.4:68752806-68752825 | MS.gene39845:CDS | 40.0% |
| !!! | GTGTTTTTGGTCTTGCTGTT+TGG | + | chr5.4:68753233-68753252 | MS.gene39845:CDS | 40.0% |
| ATCCTCATGATCCCAATCAG+AGG | - | chr5.4:68752402-68752421 | None:intergenic | 45.0% | |
| ATGCTGCTAGTGTCAGAATG+AGG | - | chr5.4:68752789-68752808 | None:intergenic | 45.0% | |
| CATTCTGACACTAGCAGCAT+TGG | + | chr5.4:68752789-68752808 | MS.gene39845:CDS | 45.0% | |
| GAAACTTGAGGAGCAGTGTT+TGG | - | chr5.4:68752954-68752973 | None:intergenic | 45.0% | |
| GAAGGGAAAAGGTAAAGCTG+AGG | + | chr5.4:68753037-68753056 | MS.gene39845:CDS | 45.0% | |
| TCCTCATGATCCCAATCAGA+GGG | - | chr5.4:68752401-68752420 | None:intergenic | 45.0% | |
| ! | GGAATTGTTGCTGGTTGTTG+AGG | - | chr5.4:68752924-68752943 | None:intergenic | 45.0% |
| ! | GGTTTCTGAGGAATTGTTGC+TGG | - | chr5.4:68752933-68752952 | None:intergenic | 45.0% |
| AACCTCAACCCGTGTGCTAA+GGG | + | chr5.4:68752698-68752717 | MS.gene39845:CDS | 50.0% | |
| ACCACTAACCCTTAGCACAC+GGG | - | chr5.4:68752709-68752728 | None:intergenic | 50.0% | |
| ACCCTCTGATTGGGATCATG+AGG | + | chr5.4:68752397-68752416 | MS.gene39845:CDS | 50.0% | |
| CACCACTAACCCTTAGCACA+CGG | - | chr5.4:68752710-68752729 | None:intergenic | 50.0% | |
| CTGCTAGTGTCAGAATGAGG+TGG | - | chr5.4:68752786-68752805 | None:intergenic | 50.0% | |
| TAACCTCAACCCGTGTGCTA+AGG | + | chr5.4:68752697-68752716 | MS.gene39845:CDS | 50.0% | |
| TCATGATCCCAATCAGAGGG+TGG | - | chr5.4:68752398-68752417 | None:intergenic | 50.0% | |
| ! | GCTGGTTGTTGAGGCTTTTG+AGG | - | chr5.4:68752915-68752934 | None:intergenic | 50.0% |
| ! | GGAGCAGTGTTTGGTTTCTG+AGG | - | chr5.4:68752945-68752964 | None:intergenic | 50.0% |
| ACCCGTGTGCTAAGGGTTAG+TGG | + | chr5.4:68752705-68752724 | MS.gene39845:CDS | 55.0% | |
| ! | AACCCTTAGCACACGGGTTG+AGG | - | chr5.4:68752703-68752722 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 68752341 | 68753319 | 68752341 | ID=MS.gene39845 |
| chr5.4 | mRNA | 68752341 | 68753319 | 68752341 | ID=MS.gene39845.t1;Parent=MS.gene39845 |
| chr5.4 | exon | 68752341 | 68752452 | 68752341 | ID=MS.gene39845.t1.exon1;Parent=MS.gene39845.t1 |
| chr5.4 | CDS | 68752341 | 68752452 | 68752341 | ID=cds.MS.gene39845.t1;Parent=MS.gene39845.t1 |
| chr5.4 | exon | 68752667 | 68753319 | 68752667 | ID=MS.gene39845.t1.exon2;Parent=MS.gene39845.t1 |
| chr5.4 | CDS | 68752667 | 68753319 | 68752667 | ID=cds.MS.gene39845.t1;Parent=MS.gene39845.t1 |
| Gene Sequence |
| Protein sequence |
