Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40272.t1 | GAU14248.1 | 82.9 | 170 | 27 | 1 | 1 | 170 | 1 | 168 | 2.30E-81 | 311.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40272.t1 | A0A392QMR0 | 82.0 | 172 | 27 | 2 | 1 | 170 | 1 | 170 | 4.0e-80 | 307.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64289 | MS.gene40272 | PPI |
| MS.gene40272 | MS.gene064976 | PPI |
| MS.gene53620 | MS.gene40272 | PPI |
| MS.gene40272 | MS.gene94983 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40272.t1 | MTR_3g109880 | 61.494 | 174 | 58 | 2 | 3 | 171 | 2 | 171 | 5.61e-71 | 212 |
| MS.gene40272.t1 | MTR_6g088445 | 45.122 | 164 | 80 | 2 | 8 | 170 | 9 | 163 | 6.56e-46 | 148 |
| MS.gene40272.t1 | MTR_6g088475 | 41.463 | 164 | 86 | 2 | 8 | 170 | 9 | 163 | 1.91e-40 | 134 |
| MS.gene40272.t1 | MTR_1g046660 | 37.037 | 162 | 96 | 3 | 4 | 164 | 3 | 159 | 1.61e-28 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40272.t1 | AT4G32870 | 46.988 | 166 | 76 | 4 | 1 | 165 | 1 | 155 | 1.76e-48 | 154 |
| MS.gene40272.t1 | AT2G25770 | 38.365 | 159 | 86 | 5 | 8 | 164 | 8 | 156 | 2.24e-33 | 116 |
| MS.gene40272.t1 | AT2G25770 | 38.365 | 159 | 86 | 5 | 8 | 164 | 8 | 156 | 2.24e-33 | 116 |
Find 39 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATACTTGTTATAAATTTGA+AGG | 0.346428 | 3.3:-91043309 | MS.gene40272:CDS |
| AATTTATAACAAGTATCAAT+TGG | 0.372630 | 3.3:+91043315 | None:intergenic |
| GAAAGTACTGCCACTGCAAA+TGG | 0.400092 | 3.3:-91043103 | MS.gene40272:CDS |
| GTTGTTATCAACAATTTCAT+AGG | 0.400837 | 3.3:+91043155 | None:intergenic |
| ATTGAGAAAGTTTCCAATGT+AGG | 0.404010 | 3.3:+91042987 | None:intergenic |
| TATAAATTTGAAGGAATTGA+AGG | 0.410358 | 3.3:-91043300 | MS.gene40272:CDS |
| TTGCCACACTATTTCCTCTT+CGG | 0.429431 | 3.3:+91043374 | None:intergenic |
| ATAGGACAAACAACGTTGTA+CGG | 0.455654 | 3.3:+91043173 | None:intergenic |
| CAAAATTTGAAATCCTACAT+TGG | 0.474889 | 3.3:-91043000 | MS.gene40272:CDS |
| ATTTATAACAAGTATCAATT+GGG | 0.476403 | 3.3:+91043316 | None:intergenic |
| TGCAAAGTCCACCCTTCCAC+AGG | 0.489996 | 3.3:+91043021 | None:intergenic |
| TTCGGTTCCTCTTACCTCAA+CGG | 0.512935 | 3.3:+91043392 | None:intergenic |
| AAAGCCACCGTTGAGGTAAG+AGG | 0.518386 | 3.3:-91043399 | MS.gene40272:CDS |
| CACTCGGCAAGAATAATGGC+AGG | 0.521022 | 3.3:-91043450 | None:intergenic |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.3:-91043059 | MS.gene40272:CDS |
| GAAGGAATTGAAGGCCAACC+TGG | 0.529028 | 3.3:-91043291 | MS.gene40272:CDS |
| CAATTGGGAGCCATTTGTGT+AGG | 0.537544 | 3.3:+91043331 | None:intergenic |
| CTTGTGATCCTGTGGAAGGG+TGG | 0.546087 | 3.3:-91043029 | MS.gene40272:CDS |
| TAGGACAAACAACGTTGTAC+GGG | 0.547037 | 3.3:+91043174 | None:intergenic |
| TGGAAACTTTCTCAATTGCA+TGG | 0.549994 | 3.3:-91042980 | MS.gene40272:CDS |
| TGTGCTTGTGATCCTGTGGA+AGG | 0.557196 | 3.3:-91043033 | MS.gene40272:CDS |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.3:-91043058 | MS.gene40272:CDS |
| GAAATTGTTGATAACAACAT+GGG | 0.602430 | 3.3:-91043150 | MS.gene40272:CDS |
| GTGCTTGTGATCCTGTGGAA+GGG | 0.612275 | 3.3:-91043032 | MS.gene40272:CDS |
| CTCGGCAAGAATAATGGCAG+GGG | 0.612851 | 3.3:-91043448 | None:intergenic |
| TGAAATTGTTGATAACAACA+TGG | 0.627477 | 3.3:-91043151 | MS.gene40272:CDS |
| GGTGATGATGCTAAACGTGT+AGG | 0.644167 | 3.3:-91043075 | MS.gene40272:CDS |
| GTACTGCCACTGCAAATGGA+CGG | 0.644735 | 3.3:-91043099 | MS.gene40272:CDS |
| GCACAATAACGAATGAGACC+AGG | 0.650328 | 3.3:+91043273 | None:intergenic |
| CATGGGATTTAAGAATTACG+TGG | 0.650792 | 3.3:-91043133 | MS.gene40272:CDS |
| GGTTCCTCTTACCTCAACGG+TGG | 0.651251 | 3.3:+91043395 | None:intergenic |
| GGAGTGTGCTTGTGATCCTG+TGG | 0.652171 | 3.3:-91043037 | MS.gene40272:CDS |
| TCACCACCGTCCATTTGCAG+TGG | 0.660619 | 3.3:+91043093 | None:intergenic |
| ACTCGGCAAGAATAATGGCA+GGG | 0.664895 | 3.3:-91043449 | None:intergenic |
| TGAGGTAAGAGGAACCGAAG+AGG | 0.684493 | 3.3:-91043388 | MS.gene40272:CDS |
| GGAAGGTAAAGCCACCGTTG+AGG | 0.711159 | 3.3:-91043406 | MS.gene40272:CDS |
| AATAACGAATGAGACCAGGT+TGG | 0.715813 | 3.3:+91043277 | None:intergenic |
| GAACCGAAGAGGAAATAGTG+TGG | 0.729750 | 3.3:-91043377 | MS.gene40272:CDS |
| CTGCCACTGCAAATGGACGG+TGG | 0.775966 | 3.3:-91043096 | MS.gene40272:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATAACAAGTATCAAT+TGG | + | chr3.3:91043062-91043081 | None:intergenic | 15.0% |
| !! | ATTTATAACAAGTATCAATT+GGG | + | chr3.3:91043061-91043080 | None:intergenic | 15.0% |
| !! | TATAAATTTGAAGGAATTGA+AGG | - | chr3.3:91043074-91043093 | MS.gene40272:CDS | 20.0% |
| !!! | GATACTTGTTATAAATTTGA+AGG | - | chr3.3:91043065-91043084 | MS.gene40272:CDS | 20.0% |
| ! | GGAAAATAAGCTAAAAAGAT+GGG | - | chr3.3:91042947-91042966 | MS.gene40272:CDS | 25.0% |
| ! | GTTGTTATCAACAATTTCAT+AGG | + | chr3.3:91043222-91043241 | None:intergenic | 25.0% |
| !! | ATTTTTGTAACCTACACAAA+TGG | - | chr3.3:91043033-91043052 | MS.gene40272:CDS | 25.0% |
| !! | CAAAATTTGAAATCCTACAT+TGG | - | chr3.3:91043374-91043393 | MS.gene40272:CDS | 25.0% |
| !! | TCTTTGAAAAACTTAACAAC+TGG | + | chr3.3:91043161-91043180 | None:intergenic | 25.0% |
| !!! | GAAATTGTTGATAACAACAT+GGG | - | chr3.3:91043224-91043243 | MS.gene40272:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAACA+TGG | - | chr3.3:91043223-91043242 | MS.gene40272:CDS | 25.0% |
| !!! | TTCATCAACACTTTTTTTTG+TGG | + | chr3.3:91043129-91043148 | None:intergenic | 25.0% |
| AATAAGCTAAAAAGATGGGA+AGG | - | chr3.3:91042951-91042970 | MS.gene40272:CDS | 30.0% | |
| ATTGAGAAAGTTTCCAATGT+AGG | + | chr3.3:91043390-91043409 | None:intergenic | 30.0% | |
| GGGAAAATAAGCTAAAAAGA+TGG | - | chr3.3:91042946-91042965 | MS.gene40272:CDS | 30.0% | |
| ! | ATATGCAAGCTCAATGTTTT+TGG | + | chr3.3:91043420-91043439 | None:intergenic | 30.0% |
| ATAGGACAAACAACGTTGTA+CGG | + | chr3.3:91043204-91043223 | None:intergenic | 35.0% | |
| CATGGGATTTAAGAATTACG+TGG | - | chr3.3:91043241-91043260 | MS.gene40272:CDS | 35.0% | |
| TGGAAACTTTCTCAATTGCA+TGG | - | chr3.3:91043394-91043413 | MS.gene40272:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.3:91043316-91043335 | MS.gene40272:CDS | 35.0% | |
| AATAACGAATGAGACCAGGT+TGG | + | chr3.3:91043100-91043119 | None:intergenic | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.3:91043315-91043334 | MS.gene40272:CDS | 40.0% | |
| TAGGACAAACAACGTTGTAC+GGG | + | chr3.3:91043203-91043222 | None:intergenic | 40.0% | |
| TTGCCACACTATTTCCTCTT+CGG | + | chr3.3:91043003-91043022 | None:intergenic | 40.0% | |
| CAATTGGGAGCCATTTGTGT+AGG | + | chr3.3:91043046-91043065 | None:intergenic | 45.0% | |
| GAACCGAAGAGGAAATAGTG+TGG | - | chr3.3:91042997-91043016 | MS.gene40272:CDS | 45.0% | |
| GCACAATAACGAATGAGACC+AGG | + | chr3.3:91043104-91043123 | None:intergenic | 45.0% | |
| TTCGGTTCCTCTTACCTCAA+CGG | + | chr3.3:91042985-91043004 | None:intergenic | 45.0% | |
| ! | GGTGATGATGCTAAACGTGT+AGG | - | chr3.3:91043299-91043318 | MS.gene40272:CDS | 45.0% |
| !! | GAAAGTACTGCCACTGCAAA+TGG | - | chr3.3:91043271-91043290 | MS.gene40272:CDS | 45.0% |
| AAAGCCACCGTTGAGGTAAG+AGG | - | chr3.3:91042975-91042994 | MS.gene40272:CDS | 50.0% | |
| GAAGGAATTGAAGGCCAACC+TGG | - | chr3.3:91043083-91043102 | MS.gene40272:CDS | 50.0% | |
| GTACTGCCACTGCAAATGGA+CGG | - | chr3.3:91043275-91043294 | MS.gene40272:CDS | 50.0% | |
| GTGCTTGTGATCCTGTGGAA+GGG | - | chr3.3:91043342-91043361 | MS.gene40272:CDS | 50.0% | |
| TGAGGTAAGAGGAACCGAAG+AGG | - | chr3.3:91042986-91043005 | MS.gene40272:CDS | 50.0% | |
| TGTGCTTGTGATCCTGTGGA+AGG | - | chr3.3:91043341-91043360 | MS.gene40272:CDS | 50.0% | |
| CTTGTGATCCTGTGGAAGGG+TGG | - | chr3.3:91043345-91043364 | MS.gene40272:CDS | 55.0% | |
| GGAAGGTAAAGCCACCGTTG+AGG | - | chr3.3:91042968-91042987 | MS.gene40272:CDS | 55.0% | |
| GGAGTGTGCTTGTGATCCTG+TGG | - | chr3.3:91043337-91043356 | MS.gene40272:CDS | 55.0% | |
| GGTTCCTCTTACCTCAACGG+TGG | + | chr3.3:91042982-91043001 | None:intergenic | 55.0% | |
| TCACCACCGTCCATTTGCAG+TGG | + | chr3.3:91043284-91043303 | None:intergenic | 55.0% | |
| TGCAAAGTCCACCCTTCCAC+AGG | + | chr3.3:91043356-91043375 | None:intergenic | 55.0% | |
| CTGCCACTGCAAATGGACGG+TGG | - | chr3.3:91043278-91043297 | MS.gene40272:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 91042939 | 91043457 | 91042939 | ID=MS.gene40272 |
| chr3.3 | mRNA | 91042939 | 91043457 | 91042939 | ID=MS.gene40272.t1;Parent=MS.gene40272 |
| chr3.3 | exon | 91042939 | 91043457 | 91042939 | ID=MS.gene40272.t1.exon1;Parent=MS.gene40272.t1 |
| chr3.3 | CDS | 91042939 | 91043457 | 91042939 | ID=cds.MS.gene40272.t1;Parent=MS.gene40272.t1 |
| Gene Sequence |
| Protein sequence |
