Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40338.t1 | XP_003603604.1 | 99.2 | 128 | 1 | 0 | 1 | 128 | 102 | 229 | 8.90E-67 | 262.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40338.t1 | O81004 | 66.9 | 127 | 42 | 0 | 1 | 127 | 104 | 230 | 1.2e-46 | 187.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40338.t1 | G7J3E8 | 99.2 | 128 | 1 | 0 | 1 | 128 | 102 | 229 | 6.4e-67 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050397 | MS.gene40338 | 0.806102 | 9.68E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene40338 | 0.813911 | 2.01E-51 | -1.69E-46 |
| MS.gene050971 | MS.gene40338 | 0.815574 | 8.62E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene40338 | -0.806438 | 8.23E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene40338 | 0.816393 | 5.66E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene40338 | 0.800537 | 1.38E-48 | -1.69E-46 |
| MS.gene058376 | MS.gene40338 | 0.80835 | 3.23E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene40338 | 0.814565 | 1.44E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATTTCGCAAATTATA+AGG | - | 20766:12959-12978 | None:intergenic | 15.0% |
| !!! | TGTATATTTTTTTGTTTGTA+GGG | + | 20766:12985-13004 | MS.gene40338:intron | 15.0% |
| !!! | TTGTATATTTTTTTGTTTGT+AGG | + | 20766:12984-13003 | MS.gene40338:intron | 15.0% |
| !!! | TTGTTTTATGTTACAAATTT+GGG | + | 20766:13114-13133 | MS.gene40338:intron | 15.0% |
| !! | AATATACAAACATGTTAGTA+TGG | + | 20766:13160-13179 | MS.gene40338:intron | 20.0% |
| !! | ATACTAACATGTTTGTATAT+TGG | - | 20766:13161-13180 | None:intergenic | 20.0% |
| !! | GAAATTAACTCATAAAATGA+GGG | - | 20766:13538-13557 | None:intergenic | 20.0% |
| !! | GGATTCCATTATTTATAAAA+AGG | + | 20766:13231-13250 | MS.gene40338:intron | 20.0% |
| !! | TCCATTATTTATAAAAAGGT+TGG | + | 20766:13235-13254 | MS.gene40338:intron | 20.0% |
| !!! | ATTTTAGTCTCATATTGTTA+AGG | + | 20766:13476-13495 | MS.gene40338:intron | 20.0% |
| !!! | CTTGTTTTATGTTACAAATT+TGG | + | 20766:13113-13132 | MS.gene40338:intron | 20.0% |
| !!! | TCCAACCTTTTTATAAATAA+TGG | - | 20766:13239-13258 | None:intergenic | 20.0% |
| !!! | TGTTTTATGTTACAAATTTG+GGG | + | 20766:13115-13134 | MS.gene40338:intron | 20.0% |
| ! | AATACATATATGCATTCCAT+TGG | - | 20766:13308-13327 | None:intergenic | 25.0% |
| ! | ACCTAATGAAAAGAACATAA+AGG | - | 20766:13842-13861 | None:intergenic | 25.0% |
| ! | ATGGAATGCATATATGTATT+TGG | + | 20766:13308-13327 | MS.gene40338:intron | 25.0% |
| ! | CGAAATTAACTCATAAAATG+AGG | - | 20766:13539-13558 | None:intergenic | 25.0% |
| ! | TAAAACAAGATTGCTTTGTA+AGG | - | 20766:13102-13121 | None:intergenic | 25.0% |
| ! | TATTTATGCCCTACAAAATT+CGG | - | 20766:13404-13423 | None:intergenic | 25.0% |
| ! | TGTAATAATAATAAGAGACC+AGG | - | 20766:12913-12932 | None:intergenic | 25.0% |
| !!! | ATTTTCGAGTTAATGATAGT+TGG | + | 20766:13027-13046 | MS.gene40338:CDS | 25.0% |
| ACAAATTTGGGGATTAACAT+TGG | + | 20766:13126-13145 | MS.gene40338:intron | 30.0% | |
| GTAATCATACAAACTAGCAT+TGG | - | 20766:13719-13738 | None:intergenic | 30.0% | |
| TCACATTGCCAAAAATTCTT+GGG | + | 20766:13282-13301 | MS.gene40338:intron | 30.0% | |
| TTGTAGTTATGCTGAAGAAA+AGG | - | 20766:13760-13779 | None:intergenic | 30.0% | |
| ! | GCCTTTATGTTCTTTTCATT+AGG | + | 20766:13838-13857 | MS.gene40338:intron | 30.0% |
| ! | TATTGGAACCGAATTTTGTA+GGG | + | 20766:13393-13412 | MS.gene40338:intron | 30.0% |
| !! | GAGTGAAATTCTAGTTCATA+TGG | + | 20766:13560-13579 | MS.gene40338:intron | 30.0% |
| !! | TATGGGTATGTATGGTTTTA+AGG | + | 20766:13071-13090 | MS.gene40338:intron | 30.0% |
| !! | TCAAGATCAATCTTTTTAGC+AGG | - | 20766:13975-13994 | None:intergenic | 30.0% |
| !! | TCTGACATTTAATTTGTCTC+TGG | + | 20766:13669-13688 | MS.gene40338:intron | 30.0% |
| AAACCCGTTTAGCATAGTAT+TGG | + | 20766:13376-13395 | MS.gene40338:intron | 35.0% | |
| AAGTTAAAGCGAGTTTCTTG+CGG | - | 20766:12882-12901 | None:intergenic | 35.0% | |
| AGCTTTCTACAAAACTGAAC+AGG | - | 20766:13797-13816 | None:intergenic | 35.0% | |
| CCAAAGACTATTTCCTTAAC+CGG | + | 20766:13907-13926 | MS.gene40338:CDS | 35.0% | |
| CTCACATTGCCAAAAATTCT+TGG | + | 20766:13281-13300 | MS.gene40338:intron | 35.0% | |
| CTCATATTGTTAAGGTTCCT+TGG | + | 20766:13484-13503 | MS.gene40338:intron | 35.0% | |
| GGTTCCAATACTATGCTAAA+CGG | - | 20766:13383-13402 | None:intergenic | 35.0% | |
| GTGTCAAACATCATAACAGT+AGG | + | 20766:13210-13229 | MS.gene40338:intron | 35.0% | |
| GTTCCAATACTATGCTAAAC+GGG | - | 20766:13382-13401 | None:intergenic | 35.0% | |
| ! | AGTTAATGATAGTTGGTACC+TGG | + | 20766:13034-13053 | MS.gene40338:CDS | 35.0% |
| ! | GCTTTAACTTCAAAGAAACC+TGG | + | 20766:12892-12911 | MS.gene40338:CDS | 35.0% |
| ! | GTATTGGAACCGAATTTTGT+AGG | + | 20766:13392-13411 | MS.gene40338:intron | 35.0% |
| !! | AATCTTTTTAGCAGGAATGC+TGG | - | 20766:13967-13986 | None:intergenic | 35.0% |
| AAACCAATGTTGACCGGTTA+AGG | - | 20766:13923-13942 | None:intergenic | 40.0% | |
| AACCATACATACCCATATCC+AGG | - | 20766:13068-13087 | None:intergenic | 40.0% | |
| AAGCGATGATTTCCCAACAT+TGG | - | 20766:12842-12861 | None:intergenic | 40.0% | |
| ATGTATACATTGCACAGCCA+AGG | - | 20766:13504-13523 | None:intergenic | 40.0% | |
| CAATCCATTCTAAGTTCCTG+CGG | - | 20766:13876-13895 | None:intergenic | 40.0% | |
| CCGGTTAAGGAAATAGTCTT+TGG | - | 20766:13910-13929 | None:intergenic | 40.0% | |
| TCGATCTCACTCTCAAAAGT+TGG | - | 20766:13357-13376 | None:intergenic | 40.0% | |
| TTGATCTTGAGTATGCTGCT+TGG | + | 20766:13985-14004 | MS.gene40338:CDS | 40.0% | |
| TTGTTGGTCGTTCCAATGTT+GGG | + | 20766:12827-12846 | MS.gene40338:CDS | 40.0% | |
| TTTCCTTAACCGGTCAACAT+TGG | + | 20766:13917-13936 | MS.gene40338:CDS | 40.0% | |
| ! | GCAGGAACTTAGAATGGATT+GGG | + | 20766:13875-13894 | MS.gene40338:CDS | 40.0% |
| ! | TCCATTGGCCCAAGAATTTT+TGG | - | 20766:13293-13312 | None:intergenic | 40.0% |
| !! | TAACCGGTCAACATTGGTTT+CGG | + | 20766:13923-13942 | MS.gene40338:CDS | 40.0% |
| !! | TGGTTGATTTGCCTGGATAT+GGG | + | 20766:13054-13073 | MS.gene40338:CDS | 40.0% |
| ACCATCGGAAGGACAATCTT+CGG | - | 20766:12791-12810 | None:intergenic | 45.0% | |
| AGACCGAAACCAATGTTGAC+CGG | - | 20766:13929-13948 | None:intergenic | 45.0% | |
| CATATCCAGGCAAATCAACC+AGG | - | 20766:13055-13074 | None:intergenic | 45.0% | |
| CGCAGGAACTTAGAATGGAT+TGG | + | 20766:13874-13893 | MS.gene40338:CDS | 45.0% | |
| CTTGTTGGTCGTTCCAATGT+TGG | + | 20766:12826-12845 | MS.gene40338:CDS | 45.0% | |
| GCCAAAAATTCTTGGGCCAA+TGG | + | 20766:13289-13308 | MS.gene40338:intron | 45.0% | |
| TGCCTGGATATGGGTATGTA+TGG | + | 20766:13063-13082 | MS.gene40338:intron | 45.0% | |
| ! | CTTGAGTATGCTGCTTGGTT+GGG | + | 20766:13990-14009 | MS.gene40338:CDS | 45.0% |
| ! | TCTTGAGTATGCTGCTTGGT+TGG | + | 20766:13989-14008 | MS.gene40338:CDS | 45.0% |
| !! | CTGGTTGATTTGCCTGGATA+TGG | + | 20766:13053-13072 | MS.gene40338:CDS | 45.0% |
| AACTCAGGAAGACCATCGGA+AGG | - | 20766:12802-12821 | None:intergenic | 50.0% | |
| ACCGAAGATTGTCCTTCCGA+TGG | + | 20766:12787-12806 | MS.gene40338:CDS | 50.0% | |
| AGCGAACTCAGGAAGACCAT+CGG | - | 20766:12806-12825 | None:intergenic | 50.0% | |
| CTTCCTGAGTTCGCTCTTGT+TGG | + | 20766:12811-12830 | MS.gene40338:CDS | 50.0% | |
| !! | TGGTACCTGGTTGATTTGCC+TGG | + | 20766:13047-13066 | MS.gene40338:CDS | 50.0% |
| CGACCAACAAGAGCGAACTC+AGG | - | 20766:12817-12836 | None:intergenic | 55.0% | |
| TCAATACCCATGCTCGCGTG+TGG | - | 20766:13450-13469 | None:intergenic | 55.0% | |
| TGCGCCGCAGGAACTTAGAA+TGG | + | 20766:13869-13888 | MS.gene40338:CDS | 55.0% | |
| CTCGCGTGTGGTGTGGGTTT+AGG | - | 20766:13438-13457 | None:intergenic | 60.0% | |
| TAGGTATGCATCTGCGCCGC+AGG | + | 20766:13857-13876 | MS.gene40338:intron | 60.0% | |
| ACCCACACCACACGCGAGCA+TGG | + | 20766:13440-13459 | MS.gene40338:intron | 65.0% | |
| ACCCATGCTCGCGTGTGGTG+TGG | - | 20766:13445-13464 | None:intergenic | 65.0% | |
| CCCACACCACACGCGAGCAT+GGG | + | 20766:13441-13460 | MS.gene40338:intron | 65.0% | |
| CCCATGCTCGCGTGTGGTGT+GGG | - | 20766:13444-13463 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 20766 | gene | 12761 | 14022 | 12761 | ID=MS.gene40338 |
| 20766 | mRNA | 12761 | 14022 | 12761 | ID=MS.gene40338.t1;Parent=MS.gene40338 |
| 20766 | exon | 12761 | 12913 | 12761 | ID=MS.gene40338.t1.exon1;Parent=MS.gene40338.t1 |
| 20766 | CDS | 12761 | 12913 | 12761 | ID=cds.MS.gene40338.t1;Parent=MS.gene40338.t1 |
| 20766 | exon | 13006 | 13075 | 13006 | ID=MS.gene40338.t1.exon2;Parent=MS.gene40338.t1 |
| 20766 | CDS | 13006 | 13075 | 13006 | ID=cds.MS.gene40338.t1;Parent=MS.gene40338.t1 |
| 20766 | exon | 13860 | 14022 | 13860 | ID=MS.gene40338.t1.exon3;Parent=MS.gene40338.t1 |
| 20766 | CDS | 13860 | 14022 | 13860 | ID=cds.MS.gene40338.t1;Parent=MS.gene40338.t1 |
| Gene Sequence |
| Protein sequence |
