Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40382.t1 | XP_003603538.1 | 91.2 | 353 | 22 | 5 | 1 | 351 | 1 | 346 | 1.40E-183 | 652.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40382.t1 | Q8LAW2 | 42.3 | 359 | 191 | 6 | 5 | 350 | 16 | 371 | 9.1e-78 | 292.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40382.t1 | G7JCD6 | 91.2 | 353 | 22 | 5 | 1 | 351 | 1 | 346 | 1.0e-183 | 652.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31040 | MS.gene40382 | PPI |
| MS.gene06482 | MS.gene40382 | PPI |
| MS.gene40382 | MS.gene013385 | PPI |
| MS.gene049362 | MS.gene40382 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40382.t1 | MTR_3g108860 | 90.652 | 353 | 24 | 4 | 1 | 351 | 1 | 346 | 0.0 | 610 |
| MS.gene40382.t1 | MTR_2g019230 | 34.828 | 379 | 217 | 8 | 1 | 351 | 29 | 405 | 5.31e-74 | 234 |
| MS.gene40382.t1 | MTR_4g114110 | 25.352 | 284 | 191 | 7 | 6 | 274 | 70 | 347 | 1.08e-23 | 101 |
| MS.gene40382.t1 | MTR_8g085600 | 25.000 | 296 | 195 | 7 | 5 | 282 | 65 | 351 | 3.05e-20 | 91.7 |
| MS.gene40382.t1 | MTR_2g008440 | 24.235 | 392 | 204 | 15 | 12 | 314 | 38 | 425 | 2.27e-19 | 89.4 |
| MS.gene40382.t1 | MTR_8g030940 | 25.397 | 252 | 166 | 7 | 17 | 252 | 25 | 270 | 8.70e-17 | 80.9 |
| MS.gene40382.t1 | MTR_3g073640 | 23.056 | 373 | 205 | 11 | 11 | 303 | 37 | 407 | 1.14e-16 | 81.3 |
| MS.gene40382.t1 | MTR_7g095220 | 29.730 | 222 | 116 | 6 | 16 | 208 | 3 | 213 | 1.77e-16 | 79.7 |
| MS.gene40382.t1 | MTR_3g085570 | 25.100 | 251 | 154 | 8 | 11 | 238 | 29 | 268 | 1.14e-15 | 77.8 |
| MS.gene40382.t1 | MTR_8g080370 | 24.615 | 260 | 160 | 9 | 4 | 238 | 36 | 284 | 5.32e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40382.t1 | AT2G24540 | 44.847 | 359 | 182 | 6 | 5 | 350 | 16 | 371 | 4.59e-105 | 313 |
| MS.gene40382.t1 | AT1G30090 | 34.986 | 363 | 213 | 7 | 6 | 351 | 42 | 398 | 3.84e-72 | 229 |
| MS.gene40382.t1 | AT1G55270 | 25.524 | 286 | 190 | 7 | 5 | 274 | 66 | 344 | 1.19e-20 | 92.8 |
| MS.gene40382.t1 | AT1G22040 | 25.132 | 378 | 200 | 13 | 6 | 303 | 32 | 406 | 3.29e-18 | 85.9 |
| MS.gene40382.t1 | AT3G61350 | 23.123 | 333 | 224 | 9 | 2 | 314 | 3 | 323 | 3.45e-18 | 85.1 |
| MS.gene40382.t1 | AT3G61350 | 23.123 | 333 | 224 | 9 | 2 | 314 | 3 | 323 | 3.45e-18 | 85.1 |
| MS.gene40382.t1 | AT1G67480 | 26.045 | 311 | 184 | 12 | 6 | 289 | 28 | 319 | 1.10e-17 | 83.6 |
| MS.gene40382.t1 | AT1G67480 | 26.045 | 311 | 184 | 12 | 6 | 289 | 28 | 319 | 1.10e-17 | 83.6 |
| MS.gene40382.t1 | AT1G67480 | 26.045 | 311 | 184 | 12 | 6 | 289 | 28 | 319 | 1.10e-17 | 83.6 |
| MS.gene40382.t1 | AT3G63220 | 28.866 | 194 | 103 | 7 | 17 | 184 | 11 | 195 | 5.77e-14 | 72.4 |
| MS.gene40382.t1 | AT3G63220 | 28.866 | 194 | 103 | 7 | 17 | 184 | 11 | 195 | 5.77e-14 | 72.4 |
| MS.gene40382.t1 | AT3G63220 | 28.866 | 194 | 103 | 7 | 17 | 184 | 4 | 188 | 6.46e-14 | 72.4 |
| MS.gene40382.t1 | AT1G16250 | 26.667 | 195 | 114 | 5 | 15 | 185 | 6 | 195 | 3.81e-13 | 70.5 |
| MS.gene40382.t1 | AT1G16250 | 26.667 | 195 | 114 | 5 | 15 | 185 | 6 | 195 | 3.81e-13 | 70.5 |
| MS.gene40382.t1 | AT2G44130 | 26.007 | 273 | 150 | 9 | 12 | 235 | 14 | 283 | 3.80e-11 | 64.3 |
| MS.gene40382.t1 | AT1G55270 | 25.359 | 209 | 134 | 6 | 82 | 274 | 16 | 218 | 9.46e-11 | 62.4 |
Find 0 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTGACAGCTAAAACAAAA+AGG | + | 24211:42193-42212 | None:intergenic | 25.0% |
| AAGATAGGTGCAAAATTTAC+GGG | - | 24211:42533-42552 | MS.gene40382:CDS | 30.0% | |
| AGTTAAAGGAAAAATCGTGA+CGG | - | 24211:42463-42482 | MS.gene40382:CDS | 30.0% | |
| GAAGATAGGTGCAAAATTTA+CGG | - | 24211:42532-42551 | MS.gene40382:CDS | 30.0% | |
| ! | TAAAGATTGCCATTGGATTT+TGG | + | 24211:42225-42244 | None:intergenic | 30.0% |
| ! | TATGAGGAAGTATAAACCAA+CGG | + | 24211:42262-42281 | None:intergenic | 30.0% |
| ! | TCTTTGAGAGTAGAAAAGAA+GGG | + | 24211:42145-42164 | None:intergenic | 30.0% |
| ! | TTCTTTGAGAGTAGAAAAGA+AGG | + | 24211:42146-42165 | None:intergenic | 30.0% |
| AAGAAACCGTTTATTGCCAA+GGG | - | 24211:42842-42861 | MS.gene40382:CDS | 35.0% | |
| AAGGATCTAAAGATTGCCAT+TGG | + | 24211:42232-42251 | None:intergenic | 35.0% | |
| ACAGCTAAAACAAAAAGGTG+TGG | + | 24211:42188-42207 | None:intergenic | 35.0% | |
| AGATAGGTGCAAAATTTACG+GGG | - | 24211:42534-42553 | MS.gene40382:CDS | 35.0% | |
| ATGAGAGAAGGAATGAAAGA+CGG | - | 24211:42683-42702 | MS.gene40382:CDS | 35.0% | |
| CACGGAAAGATATTCTTCAT+CGG | - | 24211:42341-42360 | MS.gene40382:CDS | 35.0% | |
| CATGGTTTGAAAGTGGTTAT+TGG | - | 24211:42893-42912 | MS.gene40382:CDS | 35.0% | |
| GATAATTCTCCAAATGCTTC+TGG | + | 24211:42986-43005 | None:intergenic | 35.0% | |
| GGTTTCTTCATTCCATTTCT+AGG | + | 24211:42830-42849 | None:intergenic | 35.0% | |
| !! | ACTAAAGGTTGATATGGGTA+AGG | + | 24211:42083-42102 | None:intergenic | 35.0% |
| !! | AGCGTACTAAAGGTTGATAT+GGG | + | 24211:42088-42107 | None:intergenic | 35.0% |
| !! | CAAATCTCTGCTATTTCGTT+TGG | + | 24211:42047-42066 | None:intergenic | 35.0% |
| AAAGGAAAAATCGTGACGGT+TGG | - | 24211:42467-42486 | MS.gene40382:CDS | 40.0% | |
| AAGAATATCTTTCCGTGACG+CGG | + | 24211:42338-42357 | None:intergenic | 40.0% | |
| AATTCGTATACCCAACCTCT+CGG | + | 24211:42641-42660 | None:intergenic | 40.0% | |
| ACTTCATCGTAAACCTTCAC+CGG | + | 24211:42770-42789 | None:intergenic | 40.0% | |
| ATAAACCAACGGCTAGAAGA+AGG | + | 24211:42251-42270 | None:intergenic | 40.0% | |
| CAAAATTTACGGGGGAGTTA+AGG | - | 24211:42543-42562 | MS.gene40382:CDS | 40.0% | |
| CAAACTGCATCGTTTAAGAG+AGG | + | 24211:42287-42306 | None:intergenic | 40.0% | |
| CAGTAACCTCCAAAATCCAA+TGG | - | 24211:42213-42232 | MS.gene40382:CDS | 40.0% | |
| GAAGAAACAGAGCGTACTAA+AGG | + | 24211:42098-42117 | None:intergenic | 40.0% | |
| GAAGAAACCGTTTATTGCCA+AGG | - | 24211:42841-42860 | MS.gene40382:CDS | 40.0% | |
| GAGCATGAAGAACTTATTCC+AGG | - | 24211:42017-42036 | MS.gene40382:CDS | 40.0% | |
| GATAGGTGCAAAATTTACGG+GGG | - | 24211:42535-42554 | MS.gene40382:CDS | 40.0% | |
| GATTTACCGGTGATCATTTC+CGG | + | 24211:42425-42444 | None:intergenic | 40.0% | |
| GGAAAGATATTCTTCATCGG+AGG | - | 24211:42344-42363 | MS.gene40382:CDS | 40.0% | |
| TCCAAACCCTTGGCAATAAA+CGG | + | 24211:42851-42870 | None:intergenic | 40.0% | |
| TTCACCGGTAAATCAACTTC+CGG | + | 24211:42755-42774 | None:intergenic | 40.0% | |
| TTTGGAGGATCGAATCTATG+TGG | - | 24211:42865-42884 | MS.gene40382:CDS | 40.0% | |
| ! | AGATTGCCATTGGATTTTGG+AGG | + | 24211:42222-42241 | None:intergenic | 40.0% |
| ! | GATACCGGAAGTTGATTTAC+CGG | - | 24211:42748-42767 | MS.gene40382:CDS | 40.0% |
| ! | GCTATTTCGTTTGGTAAACC+TGG | + | 24211:42038-42057 | None:intergenic | 40.0% |
| ! | TACGCTCTGTTTCTTCTTCA+TGG | - | 24211:42102-42121 | MS.gene40382:CDS | 40.0% |
| ! | TCGTTTAAGAGAGGCATATG+AGG | + | 24211:42278-42297 | None:intergenic | 40.0% |
| !! | GAGCGTACTAAAGGTTGATA+TGG | + | 24211:42089-42108 | None:intergenic | 40.0% |
| !! | GATGTTGATGATGATGTGTG+TGG | - | 24211:42929-42948 | MS.gene40382:CDS | 40.0% |
| !! | TGGGAGGGTTTTAATGATAC+CGG | - | 24211:42733-42752 | MS.gene40382:CDS | 40.0% |
| AATGAAAGACGGTTGGACAG+GGG | - | 24211:42694-42713 | MS.gene40382:CDS | 45.0% | |
| AATTTACGGGGGAGTTAAGG+AGG | - | 24211:42546-42565 | MS.gene40382:CDS | 45.0% | |
| ACCGTTTATTGCCAAGGGTT+TGG | - | 24211:42847-42866 | MS.gene40382:CDS | 45.0% | |
| ACGAATTAGAGAGTGACACG+TGG | - | 24211:42654-42673 | MS.gene40382:CDS | 45.0% | |
| ACGATGAAGTGAATGACACG+TGG | - | 24211:42780-42799 | MS.gene40382:CDS | 45.0% | |
| AGGATCGAATCTATGTGGTG+TGG | - | 24211:42870-42889 | MS.gene40382:CDS | 45.0% | |
| AGGTATGAGACGGTGGTTAA+CGG | - | 24211:42566-42585 | MS.gene40382:CDS | 45.0% | |
| ATGAAGTGAATGACACGTGG+CGG | - | 24211:42783-42802 | MS.gene40382:CDS | 45.0% | |
| CACCGGTAAATCATTCTTCG+CGG | - | 24211:42433-42452 | MS.gene40382:CDS | 45.0% | |
| CACCGTTAACAAATGGTCGA+CGG | - | 24211:42397-42416 | MS.gene40382:CDS | 45.0% | |
| GAAAGTGACACGTGGAAGAT+AGG | - | 24211:42518-42537 | MS.gene40382:CDS | 45.0% | |
| GAATGAAAGACGGTTGGACA+GGG | - | 24211:42693-42712 | MS.gene40382:CDS | 45.0% | |
| GAGAAGGAATGAAAGACGGT+TGG | - | 24211:42687-42706 | MS.gene40382:CDS | 45.0% | |
| GGTGAGAGGTTACCTAGAAA+TGG | - | 24211:42815-42834 | MS.gene40382:CDS | 45.0% | |
| GTTTATTGCCAAGGGTTTGG+AGG | - | 24211:42850-42869 | MS.gene40382:CDS | 45.0% | |
| TACACCACTTGGCAACTAGA+AGG | + | 24211:43010-43029 | None:intergenic | 45.0% | |
| TAGATCCTTCTTCTAGCCGT+TGG | - | 24211:42243-42262 | MS.gene40382:CDS | 45.0% | |
| TGCCGCGAAGAATGATTTAC+CGG | + | 24211:42438-42457 | None:intergenic | 45.0% | |
| TTCGCGGCAGAGAAAGTTAA+AGG | - | 24211:42449-42468 | MS.gene40382:CDS | 45.0% | |
| TTGGACAGGGGTAAGTGTTA+CGG | - | 24211:42706-42725 | MS.gene40382:CDS | 45.0% | |
| ! | ACCATTTGTTAACGGTGGTG+CGG | + | 24211:42394-42413 | None:intergenic | 45.0% |
| ! | AGATGTACGTAACGGAAGGA+TGG | - | 24211:42594-42613 | MS.gene40382:CDS | 45.0% |
| ! | ATCACCTTCTAGTTGCCAAG+TGG | - | 24211:43003-43022 | MS.gene40382:CDS | 45.0% |
| ! | CACCGTCGACCATTTGTTAA+CGG | + | 24211:42402-42421 | None:intergenic | 45.0% |
| ! | GGAAGTTGATTTACCGGTGA+AGG | - | 24211:42754-42773 | MS.gene40382:CDS | 45.0% |
| ! | GGAGTTAAGGAGGTATGAGA+CGG | - | 24211:42556-42575 | MS.gene40382:CDS | 45.0% |
| ! | TGATGATGTGTGTGGTGTGA+AGG | - | 24211:42937-42956 | MS.gene40382:CDS | 45.0% |
| !! | ATCTTCCACGTGTCACTTTC+CGG | + | 24211:42518-42537 | None:intergenic | 45.0% |
| AAGTGTTACGGTGTGTGGGA+GGG | - | 24211:42718-42737 | MS.gene40382:CDS | 50.0% | |
| ACCGCACCACCGTTAACAAA+TGG | - | 24211:42390-42409 | MS.gene40382:CDS | 50.0% | |
| ACGTGGAGTGAGATGAGAGA+AGG | - | 24211:42671-42690 | MS.gene40382:CDS | 50.0% | |
| ATGAGACGGTGGTTAACGGA+GGG | - | 24211:42570-42589 | MS.gene40382:CDS | 50.0% | |
| CGAATCTATGTGGTGTGGCA+TGG | - | 24211:42875-42894 | MS.gene40382:CDS | 50.0% | |
| CGGAGGGAAGATGTACGTAA+CGG | - | 24211:42586-42605 | MS.gene40382:CDS | 50.0% | |
| GATTCGATCCTCCAAACCCT+TGG | + | 24211:42861-42880 | None:intergenic | 50.0% | |
| GCGGTAAACGAATGTCGAAC+TGG | + | 24211:42375-42394 | None:intergenic | 50.0% | |
| GGAATGAAAGACGGTTGGAC+AGG | - | 24211:42692-42711 | MS.gene40382:CDS | 50.0% | |
| GGGTAAGTGTTACGGTGTGT+GGG | - | 24211:42714-42733 | MS.gene40382:CDS | 50.0% | |
| GGTGTGGCATGGTTTGAAAG+TGG | - | 24211:42886-42905 | MS.gene40382:CDS | 50.0% | |
| TAACAAATGGTCGACGGTGC+CGG | - | 24211:42403-42422 | MS.gene40382:CDS | 50.0% | |
| TAAGTGTTACGGTGTGTGGG+AGG | - | 24211:42717-42736 | MS.gene40382:CDS | 50.0% | |
| TATGAGACGGTGGTTAACGG+AGG | - | 24211:42569-42588 | MS.gene40382:CDS | 50.0% | |
| ! | CGTCGACCATTTGTTAACGG+TGG | + | 24211:42399-42418 | None:intergenic | 50.0% |
| ! | GGGAAGATGTACGTAACGGA+AGG | - | 24211:42590-42609 | MS.gene40382:CDS | 50.0% |
| ! | GTTAAGGAGGTATGAGACGG+TGG | - | 24211:42559-42578 | MS.gene40382:CDS | 50.0% |
| ! | TGTACGTAACGGAAGGATGG+TGG | - | 24211:42597-42616 | MS.gene40382:CDS | 50.0% |
| !! | TCTTCCACGTGTCACTTTCC+GGG | + | 24211:42517-42536 | None:intergenic | 50.0% |
| !! | TTGGAGGGACCAGAAGCATT+TGG | - | 24211:42974-42993 | MS.gene40382:CDS | 50.0% |
| AATGACACGTGGCGGTGTGT+GGG | - | 24211:42791-42810 | MS.gene40382:CDS | 55.0% | |
| CCTCTCGGCCTAACTGCAAA+CGG | + | 24211:42626-42645 | None:intergenic | 55.0% | |
| GGGGTAAGTGTTACGGTGTG+TGG | - | 24211:42713-42732 | MS.gene40382:CDS | 55.0% | |
| TGCAGTTAGGCCGAGAGGTT+GGG | - | 24211:42628-42647 | MS.gene40382:CDS | 55.0% | |
| TGTGTGGTGTGAAGGTGACG+TGG | - | 24211:42945-42964 | MS.gene40382:CDS | 55.0% | |
| TTGCAGTTAGGCCGAGAGGT+TGG | - | 24211:42627-42646 | MS.gene40382:CDS | 55.0% | |
| ! | ACGGTGCCGGAAATGATCAC+CGG | - | 24211:42416-42435 | MS.gene40382:CDS | 55.0% |
| ! | ACGTAACGGAAGGATGGTGG+TGG | - | 24211:42600-42619 | MS.gene40382:CDS | 55.0% |
| ! | GAAGGTGACGTGGCAGGTTT+TGG | - | 24211:42955-42974 | MS.gene40382:CDS | 55.0% |
| !! | ATCGTGACGGTTGGTGAGAG+CGG | - | 24211:42476-42495 | MS.gene40382:CDS | 55.0% |
| !!! | GTGACGTGGCAGGTTTTGGA+GGG | - | 24211:42959-42978 | MS.gene40382:CDS | 55.0% |
| ACGACCCGGAAAGTGACACG+TGG | - | 24211:42510-42529 | MS.gene40382:CDS | 60.0% | |
| CAACGAAGCGGAGGTGAAGG+AGG | + | 24211:42315-42334 | None:intergenic | 60.0% | |
| CCGTTTGCAGTTAGGCCGAG+AGG | - | 24211:42623-42642 | MS.gene40382:CDS | 60.0% | |
| GAAGCGGAGGTGAAGGAGGT+TGG | + | 24211:42311-42330 | None:intergenic | 60.0% | |
| GAATGACACGTGGCGGTGTG+TGG | - | 24211:42790-42809 | MS.gene40382:CDS | 60.0% | |
| TGGTGTGAAGGTGACGTGGC+AGG | - | 24211:42949-42968 | MS.gene40382:CDS | 60.0% | |
| ! | GGTGGTGGCCGTTTGCAGTT+AGG | - | 24211:42615-42634 | MS.gene40382:CDS | 60.0% |
| !! | GGTGACGTGGCAGGTTTTGG+AGG | - | 24211:42958-42977 | MS.gene40382:CDS | 60.0% |
| !! | TCGTGACGGTTGGTGAGAGC+GGG | - | 24211:42477-42496 | MS.gene40382:CDS | 60.0% |
| CGGCAACGAAGCGGAGGTGA+AGG | + | 24211:42318-42337 | None:intergenic | 65.0% | |
| CGGGACGGGAATCTACGACC+CGG | - | 24211:42496-42515 | MS.gene40382:CDS | 65.0% | |
| TCCGCTTCGTTGCCGCGTCA+CGG | - | 24211:42323-42342 | MS.gene40382:CDS | 65.0% | |
| TCCGTGACGCGGCAACGAAG+CGG | + | 24211:42327-42346 | None:intergenic | 65.0% | |
| !! | ACGGTTGGTGAGAGCGGGAC+GGG | - | 24211:42482-42501 | MS.gene40382:CDS | 65.0% |
| !! | GACGGTTGGTGAGAGCGGGA+CGG | - | 24211:42481-42500 | MS.gene40382:CDS | 65.0% |
| GACACGTGGCGGTGTGTGGG+TGG | - | 24211:42794-42813 | MS.gene40382:CDS | 70.0% | |
| GGCGGTGTGTGGGTGGTGAG+AGG | - | 24211:42801-42820 | MS.gene40382:CDS | 70.0% | |
| GTGACGCGGCAACGAAGCGG+AGG | + | 24211:42324-42343 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24211 | gene | 41981 | 43036 | 41981 | ID=MS.gene40382 |
| 24211 | mRNA | 41981 | 43036 | 41981 | ID=MS.gene40382.t1;Parent=MS.gene40382 |
| 24211 | exon | 41981 | 43036 | 41981 | ID=MS.gene40382.t1.exon1;Parent=MS.gene40382.t1 |
| 24211 | CDS | 41981 | 43036 | 41981 | ID=cds.MS.gene40382.t1;Parent=MS.gene40382.t1 |
| Gene Sequence |
| Protein sequence |
