Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40715.t1 | XP_003603638.1 | 97.7 | 173 | 4 | 0 | 1 | 173 | 1 | 173 | 6.60E-97 | 363.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40715.t1 | P59082 | 35.2 | 162 | 91 | 5 | 8 | 168 | 20 | 168 | 3.6e-19 | 96.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40715.t1 | G7J3I2 | 97.7 | 173 | 4 | 0 | 1 | 173 | 1 | 173 | 4.7e-97 | 363.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64289 | MS.gene40715 | PPI |
| MS.gene94983 | MS.gene40715 | PPI |
| MS.gene40715 | MS.gene064976 | PPI |
| MS.gene53620 | MS.gene40715 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40715.t1 | MTR_3g109880 | 97.688 | 173 | 4 | 0 | 1 | 173 | 1 | 173 | 1.27e-124 | 348 |
| MS.gene40715.t1 | MTR_6g088445 | 42.945 | 163 | 82 | 3 | 3 | 164 | 5 | 157 | 2.01e-41 | 137 |
| MS.gene40715.t1 | MTR_6g088475 | 39.877 | 163 | 87 | 3 | 3 | 164 | 5 | 157 | 1.29e-37 | 127 |
| MS.gene40715.t1 | MTR_1g046660 | 35.429 | 175 | 104 | 3 | 1 | 173 | 1 | 168 | 1.97e-33 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40715.t1 | AT4G32870 | 49.701 | 167 | 70 | 4 | 1 | 165 | 1 | 155 | 5.38e-56 | 174 |
| MS.gene40715.t1 | AT2G25770 | 39.157 | 166 | 89 | 4 | 1 | 164 | 1 | 156 | 2.62e-37 | 126 |
| MS.gene40715.t1 | AT2G25770 | 39.157 | 166 | 89 | 4 | 1 | 164 | 1 | 156 | 2.62e-37 | 126 |
Find 40 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGACTTTACCTTCCCATTT+TGG | 0.196562 | 3.2:+88347460 | None:intergenic |
| TATTGTGCTTCTAACATAAA+AGG | 0.298692 | 3.2:-88347304 | MS.gene40715:CDS |
| GAGAGGAATCTATACCAAAA+TGG | 0.300079 | 3.2:-88347474 | MS.gene40715:CDS |
| TGTAATGTCCACCCTTCTAT+TGG | 0.303893 | 3.2:+88347064 | None:intergenic |
| TGAGTACTGTCTTCAGTTCA+TGG | 0.311338 | 3.2:-88347023 | MS.gene40715:CDS |
| ACACCGTTGAAGAACAAGTA+TGG | 0.334958 | 3.2:-88347423 | MS.gene40715:CDS |
| TAGGACAAACAACGTTTGAT+TGG | 0.364301 | 3.2:+88347205 | None:intergenic |
| ACATGGTAACATGTGTCTAT+TGG | 0.380795 | 3.2:+88347361 | None:intergenic |
| GTTGTTATCAACAATTTCAT+AGG | 0.400837 | 3.2:+88347186 | None:intergenic |
| TTTGTTTGTGATCCAATAGA+AGG | 0.405699 | 3.2:-88347076 | MS.gene40715:CDS |
| GCCCACTTGATCGTTGTCTC+AGG | 0.413163 | 3.2:+88347253 | None:intergenic |
| ATTTCTGCAACCTACACAAA+TGG | 0.433647 | 3.2:-88347387 | MS.gene40715:CDS |
| AGAGGAATCTATACCAAAAT+GGG | 0.442772 | 3.2:-88347473 | MS.gene40715:CDS |
| TACCATGTTGAAGGTGTTCA+AGG | 0.460746 | 3.2:-88347346 | MS.gene40715:CDS |
| GAAGGTGATGCTAAAAGTGC+AGG | 0.494052 | 3.2:-88347118 | MS.gene40715:CDS |
| GTTCATGGCTAAGAAGATTG+AGG | 0.515780 | 3.2:-88347008 | MS.gene40715:CDS |
| GACACATGTTACCATGTTGA+AGG | 0.516605 | 3.2:-88347355 | MS.gene40715:CDS |
| GTGCAGGGTGTGGGATTGAG+TGG | 0.523829 | 3.2:-88347102 | MS.gene40715:CDS |
| CTATTGGAATCCATTTGTGT+AGG | 0.531264 | 3.2:+88347377 | None:intergenic |
| TGCCCATACTTGTTCTTCAA+CGG | 0.556176 | 3.2:+88347420 | None:intergenic |
| GAAATTGTTGATAACAACAT+GGG | 0.560379 | 3.2:-88347181 | MS.gene40715:CDS |
| GAAGGTGTTCAAGGCCAACC+TGG | 0.564455 | 3.2:-88347337 | MS.gene40715:CDS |
| CACCGTTGAAGAACAAGTAT+GGG | 0.572196 | 3.2:-88347422 | MS.gene40715:CDS |
| GCACAATAGCGTATGAGACC+AGG | 0.576223 | 3.2:+88347319 | None:intergenic |
| AGCCTGAGACAACGATCAAG+TGG | 0.582251 | 3.2:-88347255 | MS.gene40715:CDS |
| ATGCTAAAAGTGCAGGGTGT+GGG | 0.582963 | 3.2:-88347111 | MS.gene40715:CDS |
| TTGTTTGTGATCCAATAGAA+GGG | 0.583616 | 3.2:-88347075 | MS.gene40715:CDS |
| GGCCTTGAACACCTTCAACA+TGG | 0.588245 | 3.2:+88347344 | None:intergenic |
| TGCAGGGTGTGGGATTGAGT+GGG | 0.598045 | 3.2:-88347101 | MS.gene40715:CDS |
| TGAAATTGTTGATAACAACA+TGG | 0.609388 | 3.2:-88347182 | MS.gene40715:CDS |
| TTTGTGATCCAATAGAAGGG+TGG | 0.611273 | 3.2:-88347072 | MS.gene40715:CDS |
| GATGCTAAAAGTGCAGGGTG+TGG | 0.614021 | 3.2:-88347112 | MS.gene40715:CDS |
| GCCTGAGACAACGATCAAGT+GGG | 0.623769 | 3.2:-88347254 | MS.gene40715:CDS |
| ATCGGCGAGAGAGATGGGAG+AGG | 0.639114 | 3.2:-88347491 | None:intergenic |
| AAATTGTTGATAACAACATG+GGG | 0.648061 | 3.2:-88347180 | MS.gene40715:CDS |
| CTGAAAGTGCTTCCAAACGA+AGG | 0.654326 | 3.2:-88347136 | MS.gene40715:CDS |
| GAATCTATACCAAAATGGGA+AGG | 0.656789 | 3.2:-88347469 | MS.gene40715:CDS |
| AAGGTGATGCTAAAAGTGCA+GGG | 0.666240 | 3.2:-88347117 | MS.gene40715:CDS |
| GGAAGGTAAAGTCACTGTTG+AGG | 0.667190 | 3.2:-88347452 | MS.gene40715:CDS |
| AATAGCGTATGAGACCAGGT+TGG | 0.712406 | 3.2:+88347323 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTGTTATCAACAATTTCAT+AGG | + | chr3.2:88347274-88347293 | None:intergenic | 25.0% |
| ! | TATTGTGCTTCTAACATAAA+AGG | - | chr3.2:88347153-88347172 | MS.gene40715:CDS | 25.0% |
| !!! | AAATTGTTGATAACAACATG+GGG | - | chr3.2:88347277-88347296 | MS.gene40715:CDS | 25.0% |
| !!! | GAAATTGTTGATAACAACAT+GGG | - | chr3.2:88347276-88347295 | MS.gene40715:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAACA+TGG | - | chr3.2:88347275-88347294 | MS.gene40715:CDS | 25.0% |
| AGAGGAATCTATACCAAAAT+GGG | - | chr3.2:88346984-88347003 | MS.gene40715:CDS | 30.0% | |
| TTGTTTGTGATCCAATAGAA+GGG | - | chr3.2:88347382-88347401 | MS.gene40715:CDS | 30.0% | |
| TTTGTTTGTGATCCAATAGA+AGG | - | chr3.2:88347381-88347400 | MS.gene40715:CDS | 30.0% | |
| ACATGGTAACATGTGTCTAT+TGG | + | chr3.2:88347099-88347118 | None:intergenic | 35.0% | |
| ATTTCTGCAACCTACACAAA+TGG | - | chr3.2:88347070-88347089 | MS.gene40715:CDS | 35.0% | |
| CTATTGGAATCCATTTGTGT+AGG | + | chr3.2:88347083-88347102 | None:intergenic | 35.0% | |
| GAATCTATACCAAAATGGGA+AGG | - | chr3.2:88346988-88347007 | MS.gene40715:CDS | 35.0% | |
| GAGAGGAATCTATACCAAAA+TGG | - | chr3.2:88346983-88347002 | MS.gene40715:CDS | 35.0% | |
| TAGGACAAACAACGTTTGAT+TGG | + | chr3.2:88347255-88347274 | None:intergenic | 35.0% | |
| ACACCGTTGAAGAACAAGTA+TGG | - | chr3.2:88347034-88347053 | MS.gene40715:CDS | 40.0% | |
| CACCGTTGAAGAACAAGTAT+GGG | - | chr3.2:88347035-88347054 | MS.gene40715:CDS | 40.0% | |
| GACACATGTTACCATGTTGA+AGG | - | chr3.2:88347102-88347121 | MS.gene40715:CDS | 40.0% | |
| GTTCATGGCTAAGAAGATTG+AGG | - | chr3.2:88347449-88347468 | MS.gene40715:CDS | 40.0% | |
| TGCCCATACTTGTTCTTCAA+CGG | + | chr3.2:88347040-88347059 | None:intergenic | 40.0% | |
| TGTAATGTCCACCCTTCTAT+TGG | + | chr3.2:88347396-88347415 | None:intergenic | 40.0% | |
| TTTGTGATCCAATAGAAGGG+TGG | - | chr3.2:88347385-88347404 | MS.gene40715:CDS | 40.0% | |
| ! | AAGGTGATGCTAAAAGTGCA+GGG | - | chr3.2:88347340-88347359 | MS.gene40715:CDS | 40.0% |
| ! | CTTTTAGCATCACCTTCGTT+TGG | + | chr3.2:88347336-88347355 | None:intergenic | 40.0% |
| ! | GTGACTTTACCTTCCCATTT+TGG | + | chr3.2:88347000-88347019 | None:intergenic | 40.0% |
| ! | TGAGTACTGTCTTCAGTTCA+TGG | - | chr3.2:88347434-88347453 | MS.gene40715:CDS | 40.0% |
| !! | TACCATGTTGAAGGTGTTCA+AGG | - | chr3.2:88347111-88347130 | MS.gene40715:CDS | 40.0% |
| AATAGCGTATGAGACCAGGT+TGG | + | chr3.2:88347137-88347156 | None:intergenic | 45.0% | |
| GGAAGGTAAAGTCACTGTTG+AGG | - | chr3.2:88347005-88347024 | MS.gene40715:CDS | 45.0% | |
| ! | ATGCTAAAAGTGCAGGGTGT+GGG | - | chr3.2:88347346-88347365 | MS.gene40715:CDS | 45.0% |
| ! | GAAGGTGATGCTAAAAGTGC+AGG | - | chr3.2:88347339-88347358 | MS.gene40715:CDS | 45.0% |
| !! | CTGAAAGTGCTTCCAAACGA+AGG | - | chr3.2:88347321-88347340 | MS.gene40715:CDS | 45.0% |
| AGCCTGAGACAACGATCAAG+TGG | - | chr3.2:88347202-88347221 | MS.gene40715:CDS | 50.0% | |
| GCACAATAGCGTATGAGACC+AGG | + | chr3.2:88347141-88347160 | None:intergenic | 50.0% | |
| GCCTGAGACAACGATCAAGT+GGG | - | chr3.2:88347203-88347222 | MS.gene40715:CDS | 50.0% | |
| GGCCTTGAACACCTTCAACA+TGG | + | chr3.2:88347116-88347135 | None:intergenic | 50.0% | |
| ! | GATGCTAAAAGTGCAGGGTG+TGG | - | chr3.2:88347345-88347364 | MS.gene40715:CDS | 50.0% |
| GCCCACTTGATCGTTGTCTC+AGG | + | chr3.2:88347207-88347226 | None:intergenic | 55.0% | |
| TGCAGGGTGTGGGATTGAGT+GGG | - | chr3.2:88347356-88347375 | MS.gene40715:CDS | 55.0% | |
| !! | GAAGGTGTTCAAGGCCAACC+TGG | - | chr3.2:88347120-88347139 | MS.gene40715:CDS | 55.0% |
| GTGCAGGGTGTGGGATTGAG+TGG | - | chr3.2:88347355-88347374 | MS.gene40715:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 88346979 | 88347500 | 88346979 | ID=MS.gene40715 |
| chr3.2 | mRNA | 88346979 | 88347500 | 88346979 | ID=MS.gene40715.t1;Parent=MS.gene40715 |
| chr3.2 | exon | 88346979 | 88347500 | 88346979 | ID=MS.gene40715.t1.exon1;Parent=MS.gene40715.t1 |
| chr3.2 | CDS | 88346979 | 88347500 | 88346979 | ID=cds.MS.gene40715.t1;Parent=MS.gene40715.t1 |
| Gene Sequence |
| Protein sequence |
