Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40717.t1 | GAU14248.1 | 84.7 | 170 | 24 | 1 | 1 | 170 | 1 | 168 | 3.10E-83 | 317.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40717.t1 | A0A392QMR0 | 83.7 | 172 | 24 | 2 | 1 | 170 | 1 | 170 | 5.6e-82 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53620 | MS.gene40717 | PPI |
| MS.gene64289 | MS.gene40717 | PPI |
| MS.gene40717 | MS.gene064976 | PPI |
| MS.gene94983 | MS.gene40717 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40717.t1 | MTR_3g109880 | 62.428 | 173 | 56 | 2 | 4 | 171 | 3 | 171 | 8.83e-72 | 214 |
| MS.gene40717.t1 | MTR_6g088445 | 45.732 | 164 | 79 | 2 | 8 | 170 | 9 | 163 | 1.59e-46 | 150 |
| MS.gene40717.t1 | MTR_6g088475 | 43.125 | 160 | 81 | 2 | 8 | 166 | 9 | 159 | 4.85e-41 | 136 |
| MS.gene40717.t1 | MTR_1g046660 | 37.423 | 163 | 96 | 3 | 3 | 164 | 2 | 159 | 1.88e-29 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40717.t1 | AT4G32870 | 46.988 | 166 | 76 | 4 | 1 | 165 | 1 | 155 | 6.80e-49 | 155 |
| MS.gene40717.t1 | AT2G25770 | 36.970 | 165 | 94 | 4 | 1 | 164 | 1 | 156 | 2.38e-34 | 119 |
| MS.gene40717.t1 | AT2G25770 | 36.970 | 165 | 94 | 4 | 1 | 164 | 1 | 156 | 2.38e-34 | 119 |
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATACTTGTTATAAACTTGA+AGG | 0.368519 | 3.2:-88327988 | MS.gene40717:CDS |
| AGTTTATAACAAGTATCAAT+TGG | 0.379451 | 3.2:+88327994 | None:intergenic |
| GAAAGTACTGCCACTGCAAA+TGG | 0.400092 | 3.2:-88327782 | MS.gene40717:CDS |
| GTTGTTATCAACAATTTCAT+AGG | 0.400837 | 3.2:+88327834 | None:intergenic |
| TTGCCACACTATTTCCTCTT+CGG | 0.429431 | 3.2:+88328053 | None:intergenic |
| TATAAACTTGAAGGAATTGA+AGG | 0.434983 | 3.2:-88327979 | MS.gene40717:CDS |
| ATAGGACAAACAACGTTGTA+CGG | 0.455654 | 3.2:+88327852 | None:intergenic |
| AAAGCCACCGTTGAGGTTAG+AGG | 0.466959 | 3.2:-88328078 | MS.gene40717:CDS |
| GTTTATAACAAGTATCAATT+GGG | 0.483224 | 3.2:+88327995 | None:intergenic |
| CAAAATTTGAAATCTTACAT+TGG | 0.486428 | 3.2:-88327679 | MS.gene40717:CDS |
| TGCAAAGTCCACCCTTCCAC+AGG | 0.489996 | 3.2:+88327700 | None:intergenic |
| GAAGGAATTGAAGGCCAACC+TGG | 0.518392 | 3.2:-88327970 | MS.gene40717:CDS |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.2:-88327738 | MS.gene40717:CDS |
| CTTGTGATCCTGTGGAAGGG+TGG | 0.546087 | 3.2:-88327708 | MS.gene40717:CDS |
| TAGGACAAACAACGTTGTAC+GGG | 0.547037 | 3.2:+88327853 | None:intergenic |
| TGGAAACTTTCTCAATTGCA+TGG | 0.549994 | 3.2:-88327659 | MS.gene40717:CDS |
| TGTGCTTGTGATCCTGTGGA+AGG | 0.557196 | 3.2:-88327712 | MS.gene40717:CDS |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.2:-88327737 | MS.gene40717:CDS |
| CAATTGGGAGCCACTTGTGT+AGG | 0.573330 | 3.2:+88328010 | None:intergenic |
| TTCGGTTCCTCTAACCTCAA+CGG | 0.588456 | 3.2:+88328071 | None:intergenic |
| GAAATTGTTGATAACAACAT+GGG | 0.602430 | 3.2:-88327829 | MS.gene40717:CDS |
| GTGCTTGTGATCCTGTGGAA+GGG | 0.612275 | 3.2:-88327711 | MS.gene40717:CDS |
| TGAAATTGTTGATAACAACA+TGG | 0.627477 | 3.2:-88327830 | MS.gene40717:CDS |
| GGTGATGATGCTAAACGTGT+AGG | 0.644167 | 3.2:-88327754 | MS.gene40717:CDS |
| GTACTGCCACTGCAAATGGA+CGG | 0.644735 | 3.2:-88327778 | MS.gene40717:CDS |
| GCACAATAACGAATGAGACC+AGG | 0.650328 | 3.2:+88327952 | None:intergenic |
| GGAGTGTGCTTGTGATCCTG+TGG | 0.652171 | 3.2:-88327716 | MS.gene40717:CDS |
| TCACCACCGTCCATTTGCAG+TGG | 0.660619 | 3.2:+88327772 | None:intergenic |
| TGAGGTTAGAGGAACCGAAG+AGG | 0.671862 | 3.2:-88328067 | MS.gene40717:CDS |
| GGTTCCTCTAACCTCAACGG+TGG | 0.706209 | 3.2:+88328074 | None:intergenic |
| GGAAGGTAAAGCCACCGTTG+AGG | 0.711159 | 3.2:-88328085 | MS.gene40717:CDS |
| AATAACGAATGAGACCAGGT+TGG | 0.715813 | 3.2:+88327956 | None:intergenic |
| GAACCGAAGAGGAAATAGTG+TGG | 0.729750 | 3.2:-88328056 | MS.gene40717:CDS |
| CATGGGATTTAAGAACTACG+TGG | 0.741699 | 3.2:-88327812 | MS.gene40717:CDS |
| CTGCCACTGCAAATGGACGG+TGG | 0.775966 | 3.2:-88327775 | MS.gene40717:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTATAACAAGTATCAAT+TGG | + | chr3.2:88327741-88327760 | None:intergenic | 20.0% |
| !! | GTTTATAACAAGTATCAATT+GGG | + | chr3.2:88327740-88327759 | None:intergenic | 20.0% |
| !!! | CAAAATTTGAAATCTTACAT+TGG | - | chr3.2:88328053-88328072 | MS.gene40717:CDS | 20.0% |
| ! | AGGAAAATAAGCTAAAAAGA+TGG | - | chr3.2:88327625-88327644 | MS.gene40717:CDS | 25.0% |
| ! | GGAAAATAAGCTAAAAAGAT+GGG | - | chr3.2:88327626-88327645 | MS.gene40717:CDS | 25.0% |
| ! | GTTGTTATCAACAATTTCAT+AGG | + | chr3.2:88327901-88327920 | None:intergenic | 25.0% |
| ! | TATAAACTTGAAGGAATTGA+AGG | - | chr3.2:88327753-88327772 | MS.gene40717:CDS | 25.0% |
| !! | TCTTTGAAAAACTTAACAAC+TGG | + | chr3.2:88327840-88327859 | None:intergenic | 25.0% |
| !!! | GAAATTGTTGATAACAACAT+GGG | - | chr3.2:88327903-88327922 | MS.gene40717:CDS | 25.0% |
| !!! | GATACTTGTTATAAACTTGA+AGG | - | chr3.2:88327744-88327763 | MS.gene40717:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAACA+TGG | - | chr3.2:88327902-88327921 | MS.gene40717:CDS | 25.0% |
| AATAAGCTAAAAAGATGGGA+AGG | - | chr3.2:88327630-88327649 | MS.gene40717:CDS | 30.0% | |
| TATTGTGCTTCCACAAAAAA+AGG | - | chr3.2:88327795-88327814 | MS.gene40717:CDS | 30.0% | |
| ! | ATATGCAAGCTCAATGTTTT+TGG | + | chr3.2:88328099-88328118 | None:intergenic | 30.0% |
| ! | ATTTTTGTAACCTACACAAG+TGG | - | chr3.2:88327712-88327731 | MS.gene40717:CDS | 30.0% |
| ATAGGACAAACAACGTTGTA+CGG | + | chr3.2:88327883-88327902 | None:intergenic | 35.0% | |
| TGGAAACTTTCTCAATTGCA+TGG | - | chr3.2:88328073-88328092 | MS.gene40717:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.2:88327995-88328014 | MS.gene40717:CDS | 35.0% | |
| !!! | TTCATCAACGCCTTTTTTTG+TGG | + | chr3.2:88327808-88327827 | None:intergenic | 35.0% |
| AATAACGAATGAGACCAGGT+TGG | + | chr3.2:88327779-88327798 | None:intergenic | 40.0% | |
| CATGGGATTTAAGAACTACG+TGG | - | chr3.2:88327920-88327939 | MS.gene40717:CDS | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.2:88327994-88328013 | MS.gene40717:CDS | 40.0% | |
| TAGGACAAACAACGTTGTAC+GGG | + | chr3.2:88327882-88327901 | None:intergenic | 40.0% | |
| TTGCCACACTATTTCCTCTT+CGG | + | chr3.2:88327682-88327701 | None:intergenic | 40.0% | |
| GAACCGAAGAGGAAATAGTG+TGG | - | chr3.2:88327676-88327695 | MS.gene40717:CDS | 45.0% | |
| GCACAATAACGAATGAGACC+AGG | + | chr3.2:88327783-88327802 | None:intergenic | 45.0% | |
| TTCGGTTCCTCTAACCTCAA+CGG | + | chr3.2:88327664-88327683 | None:intergenic | 45.0% | |
| ! | GGTGATGATGCTAAACGTGT+AGG | - | chr3.2:88327978-88327997 | MS.gene40717:CDS | 45.0% |
| !! | GAAAGTACTGCCACTGCAAA+TGG | - | chr3.2:88327950-88327969 | MS.gene40717:CDS | 45.0% |
| AAAGCCACCGTTGAGGTTAG+AGG | - | chr3.2:88327654-88327673 | MS.gene40717:CDS | 50.0% | |
| CAATTGGGAGCCACTTGTGT+AGG | + | chr3.2:88327725-88327744 | None:intergenic | 50.0% | |
| GAAGGAATTGAAGGCCAACC+TGG | - | chr3.2:88327762-88327781 | MS.gene40717:CDS | 50.0% | |
| GTACTGCCACTGCAAATGGA+CGG | - | chr3.2:88327954-88327973 | MS.gene40717:CDS | 50.0% | |
| GTGCTTGTGATCCTGTGGAA+GGG | - | chr3.2:88328021-88328040 | MS.gene40717:CDS | 50.0% | |
| TGAGGTTAGAGGAACCGAAG+AGG | - | chr3.2:88327665-88327684 | MS.gene40717:CDS | 50.0% | |
| TGTGCTTGTGATCCTGTGGA+AGG | - | chr3.2:88328020-88328039 | MS.gene40717:CDS | 50.0% | |
| CTTGTGATCCTGTGGAAGGG+TGG | - | chr3.2:88328024-88328043 | MS.gene40717:CDS | 55.0% | |
| GGAAGGTAAAGCCACCGTTG+AGG | - | chr3.2:88327647-88327666 | MS.gene40717:CDS | 55.0% | |
| GGAGTGTGCTTGTGATCCTG+TGG | - | chr3.2:88328016-88328035 | MS.gene40717:CDS | 55.0% | |
| GGTTCCTCTAACCTCAACGG+TGG | + | chr3.2:88327661-88327680 | None:intergenic | 55.0% | |
| TCACCACCGTCCATTTGCAG+TGG | + | chr3.2:88327963-88327982 | None:intergenic | 55.0% | |
| TGCAAAGTCCACCCTTCCAC+AGG | + | chr3.2:88328035-88328054 | None:intergenic | 55.0% | |
| CTGCCACTGCAAATGGACGG+TGG | - | chr3.2:88327957-88327976 | MS.gene40717:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 88327618 | 88328136 | 88327618 | ID=MS.gene40717 |
| chr3.2 | mRNA | 88327618 | 88328136 | 88327618 | ID=MS.gene40717.t1;Parent=MS.gene40717 |
| chr3.2 | exon | 88327618 | 88328136 | 88327618 | ID=MS.gene40717.t1.exon1;Parent=MS.gene40717.t1 |
| chr3.2 | CDS | 88327618 | 88328136 | 88327618 | ID=cds.MS.gene40717.t1;Parent=MS.gene40717.t1 |
| Gene Sequence |
| Protein sequence |
