AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40723


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40723.t1 MTR_7g088160 48.980 196 98 2 90 285 72 265 1.88e-56 186
MS.gene40723.t1 MTR_4g063670 50.820 183 85 4 99 278 26 206 4.36e-49 165
MS.gene40723.t1 MTR_0867s0010 49.727 183 87 4 99 278 26 206 3.77e-43 150
MS.gene40723.t1 MTR_7g107340 43.386 189 78 2 84 271 69 229 2.70e-42 148
MS.gene40723.t1 MTR_4g083510 43.229 192 103 5 91 278 18 207 1.70e-38 138
MS.gene40723.t1 MTR_3g069460 47.619 147 74 3 99 242 26 172 3.29e-35 125
MS.gene40723.t1 MTR_1g051185 47.482 139 69 3 142 278 45 181 5.01e-32 119
MS.gene40723.t1 MTR_7g114170 39.316 234 91 6 35 260 7 197 2.52e-31 118
MS.gene40723.t1 MTR_6g081150 38.415 164 51 5 86 249 12 125 6.64e-23 93.2
MS.gene40723.t1 MTR_1g106885 26.316 152 106 1 116 261 144 295 6.51e-15 74.7
MS.gene40723.t1 MTR_2g022120 26.316 152 106 1 116 261 147 298 7.04e-15 74.3
MS.gene40723.t1 MTR_5g077245 26.316 152 106 1 116 261 136 287 7.97e-15 74.3
MS.gene40723.t1 MTR_7g461350 23.750 160 117 1 88 242 92 251 9.16e-15 73.9
MS.gene40723.t1 MTR_6g016670 23.750 160 117 1 88 242 92 251 9.16e-15 73.9
MS.gene40723.t1 MTR_3g464310 23.750 160 117 1 88 242 92 251 9.16e-15 73.9
MS.gene40723.t1 MTR_3g464170 23.125 160 118 1 88 242 92 251 1.43e-14 73.6
MS.gene40723.t1 MTR_1g100375 23.125 160 118 1 88 242 92 251 1.43e-14 73.6
MS.gene40723.t1 MTR_6g051405 23.125 160 118 1 88 242 92 251 1.78e-14 73.2
MS.gene40723.t1 MTR_3g450670 23.077 156 115 1 92 242 92 247 1.79e-14 73.2
MS.gene40723.t1 MTR_1g471070 23.077 156 115 1 92 242 92 247 1.79e-14 73.2
MS.gene40723.t1 MTR_6g453050 24.719 178 121 3 97 261 57 234 2.42e-14 72.4
MS.gene40723.t1 MTR_8g015740 40.127 157 64 8 1 154 1 130 2.74e-14 68.6
MS.gene40723.t1 MTR_4g026120 26.316 152 106 1 116 261 83 234 3.73e-14 72.0
MS.gene40723.t1 MTR_4g036435 26.316 152 106 1 116 261 83 234 3.73e-14 72.0
MS.gene40723.t1 MTR_6g092533 26.027 146 107 1 116 260 147 292 3.99e-14 72.0
MS.gene40723.t1 MTR_5g066280 26.316 152 106 1 116 261 83 234 4.10e-14 71.6
MS.gene40723.t1 MTR_4g013220 26.316 152 106 1 116 261 83 234 4.26e-14 71.6
MS.gene40723.t1 MTR_3g067785 26.316 152 106 1 116 261 83 234 4.30e-14 71.6
MS.gene40723.t1 MTR_2g072860 23.125 160 118 1 88 242 24 183 9.50e-14 70.5
MS.gene40723.t1 MTR_2g023560 58.824 51 21 0 118 168 57 107 5.57e-13 64.7
MS.gene40723.t1 MTR_1g075060 26.404 178 104 5 97 261 108 271 8.28e-12 65.1
MS.gene40723.t1 MTR_7g083520 25.439 114 85 0 127 240 68 181 2.54e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40723.t1 AT5G41220 39.535 172 94 4 115 276 214 385 7.53e-25 103
MS.gene40723.t1 AT5G41220 39.535 172 94 4 115 276 264 435 8.89e-25 103
MS.gene40723.t1 AT5G41220 39.535 172 94 4 115 276 206 377 8.96e-25 103
MS.gene40723.t1 AT3G47680 37.086 151 84 4 118 259 44 192 9.33e-23 95.9
MS.gene40723.t1 AT5G41240 36.686 169 96 4 115 272 264 432 7.62e-22 95.5
MS.gene40723.t1 AT1G66235 28.571 126 87 2 124 246 7 132 4.91e-13 68.2

Find 58 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCTTCTCCCTTCCATTT+AGG 0.213354 3.2:-88244842 None:intergenic
AACTCCCACGATCGGATCTT+TGG 0.301608 3.2:-88244543 None:intergenic
GAACCGTCACCGGCAGTTAA+TGG 0.321759 3.2:-88244302 None:intergenic
TGTTGAAGGATGAACCTAAA+TGG 0.353625 3.2:+88244828 MS.gene40723:CDS
TTTGAGAACCATCACCGATT+GGG 0.409235 3.2:-88244169 None:intergenic
TGTTGAATAGACATTGTCAT+TGG 0.417954 3.2:+88244672 MS.gene40723:CDS
GTTGTATTCTGAACAATCAA+TGG 0.420235 3.2:-88244936 None:intergenic
TCAACCACCTAGCGTAGAAC+TGG 0.422168 3.2:+88244349 MS.gene40723:CDS
AATTATAGTAATGGTTCCAT+TGG 0.452755 3.2:-88244136 None:intergenic
TTGTATTCTGAACAATCAAT+GGG 0.457951 3.2:-88244935 None:intergenic
AGATCAAAGACATCATCTAC+TGG 0.478245 3.2:+88244881 MS.gene40723:CDS
TTTGCATATAATTATAGTAA+TGG 0.482649 3.2:-88244145 None:intergenic
AATGTTACTTCCACGGAAGA+AGG 0.498412 3.2:+88244437 MS.gene40723:CDS
GCACGCGTGGCGGTTGTTGA+AGG 0.504466 3.2:+88244814 MS.gene40723:CDS
CTGCTAGGGAGTGGTGGTCG+TGG 0.507126 3.2:-88244212 None:intergenic
TATTGAGAACCGTCACTGCT+AGG 0.510279 3.2:-88244227 None:intergenic
AATTATATGCAAACCCCAAT+CGG 0.514611 3.2:+88244155 MS.gene40723:CDS
GCTGTTACTAACAAGAAAAG+TGG 0.520506 3.2:+88244716 MS.gene40723:CDS
GCAGTCTGAGTCCGACATCA+TGG 0.543761 3.2:+88244739 MS.gene40723:CDS
CAATCACCGCCATTAACTGC+CGG 0.543770 3.2:+88244293 MS.gene40723:CDS
AGTGTCAGTGTACTCGACTC+AGG 0.544532 3.2:+88244403 MS.gene40723:CDS
GGAGACTCATAGCGAGGGAG+TGG 0.545823 3.2:+88244624 MS.gene40723:CDS
GATTGAAACATACAAGGTCG+TGG 0.546490 3.2:-88244275 None:intergenic
TTGAGAACCATCACCGATTG+GGG 0.549171 3.2:-88244168 None:intergenic
GGTGATTCCAGTTCTACGCT+AGG 0.553730 3.2:-88244356 None:intergenic
GTCAAATGGATCACAACCAA+TGG 0.559003 3.2:+88244120 None:intergenic
ATTGAGAACCGTCACTGCTA+GGG 0.559663 3.2:-88244226 None:intergenic
GAATCTGTAGTGGATTCAAT+TGG 0.562646 3.2:-88244377 None:intergenic
GAAGGATGAACCTAAATGGA+AGG 0.565794 3.2:+88244832 MS.gene40723:CDS
ATGCAAACCCCAATCGGTGA+TGG 0.572846 3.2:+88244161 MS.gene40723:CDS
CTGTTACTAACAAGAAAAGT+GGG 0.573605 3.2:+88244717 MS.gene40723:CDS
TCTAGAAAATGTTACTTCCA+CGG 0.579593 3.2:+88244430 MS.gene40723:CDS
CCACCACTCCCTAGCAGTGA+CGG 0.586402 3.2:+88244218 MS.gene40723:CDS
CCGCCATTAACTGCCGGTGA+CGG 0.594377 3.2:+88244299 MS.gene40723:CDS
ATAAATTGCTTATTCAAACA+TGG 0.603441 3.2:+88244510 MS.gene40723:CDS
GAACCGTCACTGCTAGGGAG+TGG 0.603444 3.2:-88244221 None:intergenic
ATTGAAACATACAAGGTCGT+GGG 0.606011 3.2:-88244274 None:intergenic
AGAACGCTCTCCTTCTTCCG+TGG 0.611495 3.2:-88244447 None:intergenic
CCGTCACTGCTAGGGAGTGG+TGG 0.615284 3.2:-88244218 None:intergenic
ATGTGCATCATCCATGATGT+CGG 0.617093 3.2:-88244750 None:intergenic
AAGGATGAACCTAAATGGAA+GGG 0.623792 3.2:+88244833 MS.gene40723:CDS
TCAGTGTACTCGACTCAGGT+TGG 0.627390 3.2:+88244407 MS.gene40723:CDS
GGCGGTGATTGAAACATACA+AGG 0.629344 3.2:-88244281 None:intergenic
GATTCCAGTTCTACGCTAGG+TGG 0.631252 3.2:-88244353 None:intergenic
GATCAAAGACATCATCTACT+GGG 0.635030 3.2:+88244882 MS.gene40723:CDS
GTTTCACAATGGAGCACGCG+TGG 0.636597 3.2:+88244801 MS.gene40723:CDS
ATTTCCAAAGATCCGATCGT+GGG 0.637771 3.2:+88244539 MS.gene40723:CDS
GATGGCTATGACAAATATCG+TGG 0.640937 3.2:+88244602 MS.gene40723:CDS
TCGTGGGAGACTCATAGCGA+GGG 0.641783 3.2:+88244619 MS.gene40723:CDS
CATTTCCAAAGATCCGATCG+TGG 0.644587 3.2:+88244538 MS.gene40723:CDS
TGACACTTGAGAATCTGTAG+TGG 0.652003 3.2:-88244387 None:intergenic
ATCGTGGGAGACTCATAGCG+AGG 0.654193 3.2:+88244618 MS.gene40723:CDS
ATGGCTATGACAAATATCGT+GGG 0.656586 3.2:+88244603 MS.gene40723:CDS
ACGTAAAATACGTTTCACAA+TGG 0.660458 3.2:+88244790 MS.gene40723:CDS
GGTCCACACTCAAATCTCGA+TGG 0.664593 3.2:+88244645 MS.gene40723:CDS
TCACAATGGAGCACGCGTGG+CGG 0.673207 3.2:+88244804 MS.gene40723:CDS
CCGTCACCGGCAGTTAATGG+CGG 0.711408 3.2:-88244299 None:intergenic
ATGCCATCGAGATTTGAGTG+TGG 0.717662 3.2:-88244648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGCATATAATTATAGTAA+TGG - chr3.2:88244148-88244167 None:intergenic 15.0%
!! ATAAATTGCTTATTCAAACA+TGG + chr3.2:88244510-88244529 MS.gene40723:CDS 20.0%
! AATTATAGTAATGGTTCCAT+TGG - chr3.2:88244139-88244158 None:intergenic 25.0%
! TTGTATTCTGAACAATCAAT+GGG - chr3.2:88244938-88244957 None:intergenic 25.0%
AATTATATGCAAACCCCAAT+CGG + chr3.2:88244155-88244174 MS.gene40723:CDS 30.0%
ACGTAAAATACGTTTCACAA+TGG + chr3.2:88244790-88244809 MS.gene40723:CDS 30.0%
CTAGGTGGTTGAAAAATAAA+AGG - chr3.2:88244341-88244360 None:intergenic 30.0%
CTGTTACTAACAAGAAAAGT+GGG + chr3.2:88244717-88244736 MS.gene40723:CDS 30.0%
GTTGTATTCTGAACAATCAA+TGG - chr3.2:88244939-88244958 None:intergenic 30.0%
TGTTGAATAGACATTGTCAT+TGG + chr3.2:88244672-88244691 MS.gene40723:CDS 30.0%
! TCTAGAAAATGTTACTTCCA+CGG + chr3.2:88244430-88244449 MS.gene40723:CDS 30.0%
!!! GTTTTGAGACATAAAAGGTT+GGG - chr3.2:88244255-88244274 None:intergenic 30.0%
!!! TTTTGAGACATAAAAGGTTG+GGG - chr3.2:88244254-88244273 None:intergenic 30.0%
AAGGATGAACCTAAATGGAA+GGG + chr3.2:88244833-88244852 MS.gene40723:CDS 35.0%
AGATCAAAGACATCATCTAC+TGG + chr3.2:88244881-88244900 MS.gene40723:CDS 35.0%
ATGGCTATGACAAATATCGT+GGG + chr3.2:88244603-88244622 MS.gene40723:CDS 35.0%
ATTGAAACATACAAGGTCGT+GGG - chr3.2:88244277-88244296 None:intergenic 35.0%
GATCAAAGACATCATCTACT+GGG + chr3.2:88244882-88244901 MS.gene40723:CDS 35.0%
GCTGTTACTAACAAGAAAAG+TGG + chr3.2:88244716-88244735 MS.gene40723:CDS 35.0%
GGTTGAAAAATAAAAGGTCG+TGG - chr3.2:88244335-88244354 None:intergenic 35.0%
TGTTGAAGGATGAACCTAAA+TGG + chr3.2:88244828-88244847 MS.gene40723:CDS 35.0%
! ACCAAAAAGCAGATAGCTTT+TGG + chr3.2:88244567-88244586 MS.gene40723:CDS 35.0%
! CAAAAAGCAGATAGCTTTTG+GGG + chr3.2:88244569-88244588 MS.gene40723:CDS 35.0%
! CCAAAAAGCAGATAGCTTTT+GGG + chr3.2:88244568-88244587 MS.gene40723:CDS 35.0%
! GAACAATCAATGGGATTTTC+TGG - chr3.2:88244929-88244948 None:intergenic 35.0%
!! GAATCTGTAGTGGATTCAAT+TGG - chr3.2:88244380-88244399 None:intergenic 35.0%
!!! ACCTGAAACGTTGTTTTTTC+TGG - chr3.2:88244475-88244494 None:intergenic 35.0%
!!! AGACATTGTCATTGGTTTTG+CGG + chr3.2:88244680-88244699 MS.gene40723:CDS 35.0%
!!! GGTTTTGAGACATAAAAGGT+TGG - chr3.2:88244256-88244275 None:intergenic 35.0%
ATGTGCATCATCCATGATGT+CGG - chr3.2:88244753-88244772 None:intergenic 40.0%
ATTGCTTCTCCCTTCCATTT+AGG - chr3.2:88244845-88244864 None:intergenic 40.0%
ATTTCCAAAGATCCGATCGT+GGG + chr3.2:88244539-88244558 MS.gene40723:CDS 40.0%
GAAGGATGAACCTAAATGGA+AGG + chr3.2:88244832-88244851 MS.gene40723:CDS 40.0%
GATGGCTATGACAAATATCG+TGG + chr3.2:88244602-88244621 MS.gene40723:CDS 40.0%
GATTGAAACATACAAGGTCG+TGG - chr3.2:88244278-88244297 None:intergenic 40.0%
GCCAGAAAAAACAACGTTTC+AGG + chr3.2:88244471-88244490 MS.gene40723:CDS 40.0%
TGACACTTGAGAATCTGTAG+TGG - chr3.2:88244390-88244409 None:intergenic 40.0%
! AATGTTACTTCCACGGAAGA+AGG + chr3.2:88244437-88244456 MS.gene40723:CDS 40.0%
! TTTGAGAACCATCACCGATT+GGG - chr3.2:88244172-88244191 None:intergenic 40.0%
!! CCCAAAAGCTATCTGCTTTT+TGG - chr3.2:88244571-88244590 None:intergenic 40.0%
!! TTTTGAGAACCATCACCGAT+TGG - chr3.2:88244173-88244192 None:intergenic 40.0%
!!! CGTGGGTTTTGAGACATAAA+AGG - chr3.2:88244260-88244279 None:intergenic 40.0%
!!! TAGCTTTTGGGGTAGAATCA+AGG + chr3.2:88244580-88244599 MS.gene40723:CDS 40.0%
!!! TTTTGGGGTAGAATCAAGGA+TGG + chr3.2:88244584-88244603 MS.gene40723:CDS 40.0%
ATGCCATCGAGATTTGAGTG+TGG - chr3.2:88244651-88244670 None:intergenic 45.0%
ATTGAGAACCGTCACTGCTA+GGG - chr3.2:88244229-88244248 None:intergenic 45.0%
CATTTCCAAAGATCCGATCG+TGG + chr3.2:88244538-88244557 MS.gene40723:CDS 45.0%
GGCGGTGATTGAAACATACA+AGG - chr3.2:88244284-88244303 None:intergenic 45.0%
TATTGAGAACCGTCACTGCT+AGG - chr3.2:88244230-88244249 None:intergenic 45.0%
! TTGAGAACCATCACCGATTG+GGG - chr3.2:88244171-88244190 None:intergenic 45.0%
! TTTTGGTCAACTCCCACGAT+CGG - chr3.2:88244554-88244573 None:intergenic 45.0%
!! TGGATTTTGAGAACCGTCAC+CGG - chr3.2:88244315-88244334 None:intergenic 45.0%
AACTCCCACGATCGGATCTT+TGG - chr3.2:88244546-88244565 None:intergenic 50.0%
AGTGTCAGTGTACTCGACTC+AGG + chr3.2:88244403-88244422 MS.gene40723:CDS 50.0%
ATGCAAACCCCAATCGGTGA+TGG + chr3.2:88244161-88244180 MS.gene40723:CDS 50.0%
CAATCACCGCCATTAACTGC+CGG + chr3.2:88244293-88244312 MS.gene40723:CDS 50.0%
GATTCCAGTTCTACGCTAGG+TGG - chr3.2:88244356-88244375 None:intergenic 50.0%
GGTCCACACTCAAATCTCGA+TGG + chr3.2:88244645-88244664 MS.gene40723:CDS 50.0%
GGTGATTCCAGTTCTACGCT+AGG - chr3.2:88244359-88244378 None:intergenic 50.0%
TCAACCACCTAGCGTAGAAC+TGG + chr3.2:88244349-88244368 MS.gene40723:CDS 50.0%
TCAGTGTACTCGACTCAGGT+TGG + chr3.2:88244407-88244426 MS.gene40723:CDS 50.0%
AGAACGCTCTCCTTCTTCCG+TGG - chr3.2:88244450-88244469 None:intergenic 55.0%
ATCGTGGGAGACTCATAGCG+AGG + chr3.2:88244618-88244637 MS.gene40723:CDS 55.0%
GAACCGTCACCGGCAGTTAA+TGG - chr3.2:88244305-88244324 None:intergenic 55.0%
GCAGTCTGAGTCCGACATCA+TGG + chr3.2:88244739-88244758 MS.gene40723:CDS 55.0%
GTTTCACAATGGAGCACGCG+TGG + chr3.2:88244801-88244820 MS.gene40723:CDS 55.0%
TCGTGGGAGACTCATAGCGA+GGG + chr3.2:88244619-88244638 MS.gene40723:CDS 55.0%
CCACCACTCCCTAGCAGTGA+CGG + chr3.2:88244218-88244237 MS.gene40723:CDS 60.0%
CCGCCATTAACTGCCGGTGA+CGG + chr3.2:88244299-88244318 MS.gene40723:CDS 60.0%
CCGTCACCGGCAGTTAATGG+CGG - chr3.2:88244302-88244321 None:intergenic 60.0%
GAACCGTCACTGCTAGGGAG+TGG - chr3.2:88244224-88244243 None:intergenic 60.0%
GGAGACTCATAGCGAGGGAG+TGG + chr3.2:88244624-88244643 MS.gene40723:CDS 60.0%
TCACAATGGAGCACGCGTGG+CGG + chr3.2:88244804-88244823 MS.gene40723:CDS 60.0%
CCGTCACTGCTAGGGAGTGG+TGG - chr3.2:88244221-88244240 None:intergenic 65.0%
CTGCTAGGGAGTGGTGGTCG+TGG - chr3.2:88244215-88244234 None:intergenic 65.0%
GCACGCGTGGCGGTTGTTGA+AGG + chr3.2:88244814-88244833 MS.gene40723:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 88244125 88244982 88244125 ID=MS.gene40723
chr3.2 mRNA 88244125 88244982 88244125 ID=MS.gene40723.t1;Parent=MS.gene40723
chr3.2 exon 88244125 88244982 88244125 ID=MS.gene40723.t1.exon1;Parent=MS.gene40723.t1
chr3.2 CDS 88244125 88244982 88244125 ID=cds.MS.gene40723.t1;Parent=MS.gene40723.t1
Gene Sequence

>MS.gene40723

ATGGATCACAACCAATGGAACCATTACTATAATTATATGCAAACCCCAATCGGTGATGGTTCTCAAAATTTTCAATCTTTTACGTATCCACGACCACCACTCCCTAGCAGTGACGGTTCTCAATATCCCCAACCTTTTATGTCTCAAAACCCACGACCTTGTATGTTTCAATCACCGCCATTAACTGCCGGTGACGGTTCTCAAAATCCACGACCTTTTATTTTTCAACCACCTAGCGTAGAACTGGAATCACCAATTGAATCCACTACAGATTCTCAAGTGTCAGTGTACTCGACTCAGGTTGGTCTAGAAAATGTTACTTCCACGGAAGAAGGAGAGCGTTCTAGCCAGAAAAAACAACGTTTCAGGTTCTCAGATGAAGAAGATAAATTGCTTATTCAAACATGGCTCAACATTTCCAAAGATCCGATCGTGGGAGTTGACCAAAAAGCAGATAGCTTTTGGGGTAGAATCAAGGATGGCTATGACAAATATCGTGGGAGACTCATAGCGAGGGAGTGGTCCACACTCAAATCTCGATGGCATTTGTTGAATAGACATTGTCATTGGTTTTGCGGCTCTTACAAACTAGCTGTTACTAACAAGAAAAGTGGGCAGTCTGAGTCCGACATCATGGATGATGCACATATGCTTTTTGTACAAGTACGTAAAATACGTTTCACAATGGAGCACGCGTGGCGGTTGTTGAAGGATGAACCTAAATGGAAGGGAGAAGCAATGAACAACTCTTCAAAGAGATCAAAGACATCATCTACTGGGACATACTCATCTACATCTAATCCAGAAAATCCCATTGATTGTTCAGAATACAACAATGCATCTCAAACAATGTTGTAA

Protein sequence

>MS.gene40723.t1

MDHNQWNHYYNYMQTPIGDGSQNFQSFTYPRPPLPSSDGSQYPQPFMSQNPRPCMFQSPPLTAGDGSQNPRPFIFQPPSVELESPIESTTDSQVSVYSTQVGLENVTSTEEGERSSQKKQRFRFSDEEDKLLIQTWLNISKDPIVGVDQKADSFWGRIKDGYDKYRGRLIAREWSTLKSRWHLLNRHCHWFCGSYKLAVTNKKSGQSESDIMDDAHMLFVQVRKIRFTMEHAWRLLKDEPKWKGEAMNNSSKRSKTSSTGTYSSTSNPENPIDCSEYNNASQTML