Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41006.t1 | KEH34366.1 | 71.1 | 228 | 53 | 3 | 1 | 227 | 4 | 219 | 9.30E-75 | 290 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41006.t1 | A0A072UY95 | 71.1 | 228 | 53 | 3 | 1 | 227 | 4 | 219 | 6.7e-75 | 290.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene41006 | 0.870645 | 1.25E-66 | -1.69E-46 |
| MS.gene053491 | MS.gene41006 | 0.840923 | 6.49E-58 | -1.69E-46 |
| MS.gene06044 | MS.gene41006 | 0.849005 | 4.27E-60 | -1.69E-46 |
| MS.gene061245 | MS.gene41006 | 0.834895 | 2.30E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41006.t1 | MTR_3g465350 | 77.193 | 228 | 39 | 3 | 1 | 227 | 4 | 219 | 6.09e-120 | 340 |
| MS.gene41006.t1 | MTR_3g465380 | 38.953 | 172 | 91 | 5 | 24 | 187 | 38 | 203 | 2.07e-22 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATAGATCGTCAATATT+AGG | 0.196396 | 1.3:-41078769 | None:intergenic |
| AAATCCAAGTGAAGAAATTA+AGG | 0.250461 | 1.3:+41078299 | MS.gene41006:CDS |
| TGAGTCCGAGTCTCGATTAA+AGG | 0.273789 | 1.3:-41077805 | None:intergenic |
| TCCTCTTAGGACCCTTGTTT+TGG | 0.280514 | 1.3:-41077305 | None:intergenic |
| GAACTAGCACAGCTGATTAA+TGG | 0.293544 | 1.3:+41078349 | MS.gene41006:CDS |
| TATTTCAGTCTGATCCTCTT+AGG | 0.304174 | 1.3:-41077318 | None:intergenic |
| CTCCAACATGTGGTTCAGTT+TGG | 0.312413 | 1.3:+41077223 | MS.gene41006:CDS |
| CCAACATCAAACGGTGCTTT+TGG | 0.325999 | 1.3:-41078059 | None:intergenic |
| GTTTGCAGTAGAGTGGTTAA+AGG | 0.332534 | 1.3:-41077253 | None:intergenic |
| TCCAACATGTGGTTCAGTTT+GGG | 0.349563 | 1.3:+41077224 | MS.gene41006:CDS |
| TTGTTCTTACCTTGATCATA+TGG | 0.360817 | 1.3:-41078430 | None:intergenic |
| AGAACACAACAACATGGAAT+TGG | 0.366539 | 1.3:+41078723 | MS.gene41006:CDS |
| ACATAGGTGGCGTCCACATT+TGG | 0.369942 | 1.3:-41078684 | None:intergenic |
| ATAATTTGAGTCTAAATCAT+TGG | 0.376358 | 1.3:-41078398 | None:intergenic |
| GAAATAGATCGTCAATATTA+GGG | 0.384297 | 1.3:-41078768 | None:intergenic |
| GATACAAAGGTGGTTCTGTT+TGG | 0.388046 | 1.3:-41077200 | None:intergenic |
| AACTAGCACAGCTGATTAAT+GGG | 0.393662 | 1.3:+41078350 | MS.gene41006:CDS |
| GAACACAACAACATGGAATT+GGG | 0.459020 | 1.3:+41078724 | MS.gene41006:CDS |
| TGCAAACTTAGGAGCCAACT+TGG | 0.462105 | 1.3:+41077269 | MS.gene41006:CDS |
| GTTCCCTTAATTTCTTCACT+TGG | 0.466132 | 1.3:-41078303 | None:intergenic |
| GGAATTGGGCATGGAATGGA+TGG | 0.466811 | 1.3:+41078738 | MS.gene41006:CDS |
| GTTTCAAAACCATATGATCA+AGG | 0.472120 | 1.3:+41078421 | MS.gene41006:CDS |
| GTTTCGTGCTCATCCGACAT+AGG | 0.491148 | 1.3:-41078700 | None:intergenic |
| CCAAAAGCACCGTTTGATGT+TGG | 0.496047 | 1.3:+41078059 | MS.gene41006:CDS |
| AATCCAAGTGAAGAAATTAA+GGG | 0.496720 | 1.3:+41078300 | MS.gene41006:CDS |
| GTTGGAGCGTGTGACGTTGA+TGG | 0.506274 | 1.3:+41078077 | MS.gene41006:CDS |
| GTCACTATTAAGACCCAAGT+TGG | 0.507718 | 1.3:-41077283 | None:intergenic |
| AACCACTCTACTGCAAACTT+AGG | 0.532758 | 1.3:+41077258 | MS.gene41006:CDS |
| CTCCTAAGTTTGCAGTAGAG+TGG | 0.536634 | 1.3:-41077260 | None:intergenic |
| AATGTGGACGCCACCTATGT+CGG | 0.552697 | 1.3:+41078687 | MS.gene41006:CDS |
| AAAGGTGGTTCTGTTTGGAA+AGG | 0.562830 | 1.3:-41077195 | None:intergenic |
| TCACACGCTCCAACATCAAA+CGG | 0.566826 | 1.3:-41078068 | None:intergenic |
| ACATGGAATTGGGCATGGAA+TGG | 0.568585 | 1.3:+41078734 | MS.gene41006:CDS |
| TCTTAATAGTGACCAAAACA+AGG | 0.569518 | 1.3:+41077293 | MS.gene41006:CDS |
| GAGTCTAAATCATTGGACAT+TGG | 0.575430 | 1.3:-41078391 | None:intergenic |
| GATCAAGACGGAAGGAAGAT+CGG | 0.579150 | 1.3:+41077835 | MS.gene41006:CDS |
| GACTATTACAAAGCCAAATG+TGG | 0.588677 | 1.3:+41078671 | MS.gene41006:CDS |
| CAACAACATGGAATTGGGCA+TGG | 0.590352 | 1.3:+41078729 | MS.gene41006:CDS |
| ACCAAAACAAGGGTCCTAAG+AGG | 0.590710 | 1.3:+41077304 | MS.gene41006:CDS |
| GTTCTGTTTGGAAAGGAACA+TGG | 0.591842 | 1.3:-41077188 | None:intergenic |
| TCCCAAACTGAACCACATGT+TGG | 0.599459 | 1.3:-41077225 | None:intergenic |
| CAAACTGAACCACATGTTGG+AGG | 0.601431 | 1.3:-41077222 | None:intergenic |
| CACTTAGAGAACATAATGGA+GGG | 0.621926 | 1.3:+41077156 | None:intergenic |
| CCACATGTTGGAGGATACAA+AGG | 0.621996 | 1.3:-41077213 | None:intergenic |
| AGCTCATAGATCAAGACGGA+AGG | 0.626708 | 1.3:+41077827 | MS.gene41006:CDS |
| AAAGAAGCACATCTTAAAGA+CGG | 0.628544 | 1.3:+41078120 | MS.gene41006:CDS |
| CGAAACAGAACACAACAACA+TGG | 0.635296 | 1.3:+41078717 | MS.gene41006:CDS |
| CTACGTCGATCAACAAGCTG+AGG | 0.639200 | 1.3:+41078327 | MS.gene41006:CDS |
| TCGTGCTCATCCGACATAGG+TGG | 0.642330 | 1.3:-41078697 | None:intergenic |
| CCTTTGTATCCTCCAACATG+TGG | 0.647083 | 1.3:+41077213 | MS.gene41006:CDS |
| TGAAACCTTTAATCGAGACT+CGG | 0.649080 | 1.3:+41077800 | MS.gene41006:CDS |
| GCAAACTTAGGAGCCAACTT+GGG | 0.651364 | 1.3:+41077270 | MS.gene41006:CDS |
| ACTCAGCTCATAGATCAAGA+CGG | 0.691685 | 1.3:+41077823 | MS.gene41006:CDS |
| CTTAATAGTGACCAAAACAA+GGG | 0.698882 | 1.3:+41077294 | MS.gene41006:CDS |
| CATGTTGGAGGATACAAAGG+TGG | 0.702436 | 1.3:-41077210 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTATTATTTATTGGTTT+TGG | + | chr1.3:41078540-41078559 | MS.gene41006:intron | 10.0% |
| !! | CTCTCAAAAAAAAAAAATTT+GGG | + | chr1.3:41077431-41077450 | MS.gene41006:intron | 15.0% |
| !! | GTATGCTAATTATTATTTAT+TGG | + | chr1.3:41078534-41078553 | MS.gene41006:intron | 15.0% |
| !! | TAAAAAAGTTAAAGAAATTG+AGG | + | chr1.3:41077875-41077894 | MS.gene41006:CDS | 15.0% |
| !! | TCTCAAAAAAAAAAAATTTG+GGG | + | chr1.3:41077432-41077451 | MS.gene41006:intron | 15.0% |
| !! | TCTCTCAAAAAAAAAAAATT+TGG | + | chr1.3:41077430-41077449 | MS.gene41006:intron | 15.0% |
| !!! | TTACTTTTTTAAAATGACAT+AGG | + | chr1.3:41078649-41078668 | MS.gene41006:intron | 15.0% |
| !!! | TTTAACTTTTTTACTCAAAT+AGG | - | chr1.3:41077868-41077887 | None:intergenic | 15.0% |
| !!! | TTTGACATTTTAAATAATTG+TGG | + | chr1.3:41077401-41077420 | MS.gene41006:intron | 15.0% |
| !! | AAAAGAATTAATTAGTCACT+AGG | - | chr1.3:41078574-41078593 | None:intergenic | 20.0% |
| !! | ACACACAGATATATATATTA+AGG | + | chr1.3:41077461-41077480 | MS.gene41006:intron | 20.0% |
| !! | ATAATTTGAGTCTAAATCAT+TGG | - | chr1.3:41078401-41078420 | None:intergenic | 20.0% |
| !! | TCATCATAATCTAAATTTCA+AGG | - | chr1.3:41077700-41077719 | None:intergenic | 20.0% |
| !! | TTAAAATGTCAAATATCACA+AGG | - | chr1.3:41077394-41077413 | None:intergenic | 20.0% |
| !!! | CAACATATTAGTTTTTGAAA+AGG | - | chr1.3:41078500-41078519 | None:intergenic | 20.0% |
| ! | AAATCCAAGTGAAGAAATTA+AGG | + | chr1.3:41078299-41078318 | MS.gene41006:CDS | 25.0% |
| ! | AATCCAAGTGAAGAAATTAA+GGG | + | chr1.3:41078300-41078319 | MS.gene41006:CDS | 25.0% |
| ! | ACTTTCAGCTGATAATTAAT+GGG | - | chr1.3:41078269-41078288 | None:intergenic | 25.0% |
| ! | AGAAATAGATCGTCAATATT+AGG | - | chr1.3:41078772-41078791 | None:intergenic | 25.0% |
| ! | AGACTGAAATACATGTAATT+TGG | + | chr1.3:41077330-41077349 | MS.gene41006:intron | 25.0% |
| ! | CATAGCTCAATATATTGATA+AGG | + | chr1.3:41078203-41078222 | MS.gene41006:intron | 25.0% |
| ! | GAAATAGATCGTCAATATTA+GGG | - | chr1.3:41078771-41078790 | None:intergenic | 25.0% |
| CACTTTCAGCTGATAATTAA+TGG | - | chr1.3:41078270-41078289 | None:intergenic | 30.0% | |
| CTTAATAGTGACCAAAACAA+GGG | + | chr1.3:41077294-41077313 | MS.gene41006:CDS | 30.0% | |
| GTTTCAAAACCATATGATCA+AGG | + | chr1.3:41078421-41078440 | MS.gene41006:CDS | 30.0% | |
| TCTTAATAGTGACCAAAACA+AGG | + | chr1.3:41077293-41077312 | MS.gene41006:CDS | 30.0% | |
| TTGTTCTTACCTTGATCATA+TGG | - | chr1.3:41078433-41078452 | None:intergenic | 30.0% | |
| ! | AAAGAAGCACATCTTAAAGA+CGG | + | chr1.3:41078120-41078139 | MS.gene41006:CDS | 30.0% |
| AACTAGCACAGCTGATTAAT+GGG | + | chr1.3:41078350-41078369 | MS.gene41006:CDS | 35.0% | |
| AGAACACAACAACATGGAAT+TGG | + | chr1.3:41078723-41078742 | MS.gene41006:CDS | 35.0% | |
| CGCTCATCATAAATATATGC+AGG | + | chr1.3:41077993-41078012 | MS.gene41006:intron | 35.0% | |
| GAACACAACAACATGGAATT+GGG | + | chr1.3:41078724-41078743 | MS.gene41006:CDS | 35.0% | |
| GACTATTACAAAGCCAAATG+TGG | + | chr1.3:41078671-41078690 | MS.gene41006:CDS | 35.0% | |
| GAGTCTAAATCATTGGACAT+TGG | - | chr1.3:41078394-41078413 | None:intergenic | 35.0% | |
| GTTCCCTTAATTTCTTCACT+TGG | - | chr1.3:41078306-41078325 | None:intergenic | 35.0% | |
| TATTTCAGTCTGATCCTCTT+AGG | - | chr1.3:41077321-41077340 | None:intergenic | 35.0% | |
| TGAAACCTTTAATCGAGACT+CGG | + | chr1.3:41077800-41077819 | MS.gene41006:CDS | 35.0% | |
| AACCACTCTACTGCAAACTT+AGG | + | chr1.3:41077258-41077277 | MS.gene41006:CDS | 40.0% | |
| ACACATGCATTATGAGATGC+TGG | - | chr1.3:41077740-41077759 | None:intergenic | 40.0% | |
| ACTCAGCTCATAGATCAAGA+CGG | + | chr1.3:41077823-41077842 | MS.gene41006:CDS | 40.0% | |
| CATGCATTCACCAAAGTCAA+TGG | + | chr1.3:41077605-41077624 | MS.gene41006:intron | 40.0% | |
| CGAAACAGAACACAACAACA+TGG | + | chr1.3:41078717-41078736 | MS.gene41006:CDS | 40.0% | |
| GAACTAGCACAGCTGATTAA+TGG | + | chr1.3:41078349-41078368 | MS.gene41006:CDS | 40.0% | |
| GTCACTATTAAGACCCAAGT+TGG | - | chr1.3:41077286-41077305 | None:intergenic | 40.0% | |
| GTTCTGTTTGGAAAGGAACA+TGG | - | chr1.3:41077191-41077210 | None:intergenic | 40.0% | |
| GTTTGCAGTAGAGTGGTTAA+AGG | - | chr1.3:41077256-41077275 | None:intergenic | 40.0% | |
| TCCAACATGTGGTTCAGTTT+GGG | + | chr1.3:41077224-41077243 | MS.gene41006:CDS | 40.0% | |
| ! | AAAGGTGGTTCTGTTTGGAA+AGG | - | chr1.3:41077198-41077217 | None:intergenic | 40.0% |
| ! | GATACAAAGGTGGTTCTGTT+TGG | - | chr1.3:41077203-41077222 | None:intergenic | 40.0% |
| ACATGGAATTGGGCATGGAA+TGG | + | chr1.3:41078734-41078753 | MS.gene41006:CDS | 45.0% | |
| ACCAAAACAAGGGTCCTAAG+AGG | + | chr1.3:41077304-41077323 | MS.gene41006:CDS | 45.0% | |
| AGATCGACATCAAACGGTTC+AGG | + | chr1.3:41077632-41077651 | MS.gene41006:intron | 45.0% | |
| AGCTCATAGATCAAGACGGA+AGG | + | chr1.3:41077827-41077846 | MS.gene41006:CDS | 45.0% | |
| CAAACTGAACCACATGTTGG+AGG | - | chr1.3:41077225-41077244 | None:intergenic | 45.0% | |
| CAACAACATGGAATTGGGCA+TGG | + | chr1.3:41078729-41078748 | MS.gene41006:CDS | 45.0% | |
| CATGTTGGAGGATACAAAGG+TGG | - | chr1.3:41077213-41077232 | None:intergenic | 45.0% | |
| CCACATGTTGGAGGATACAA+AGG | - | chr1.3:41077216-41077235 | None:intergenic | 45.0% | |
| CCTTTGTATCCTCCAACATG+TGG | + | chr1.3:41077213-41077232 | MS.gene41006:CDS | 45.0% | |
| CTCCAACATGTGGTTCAGTT+TGG | + | chr1.3:41077223-41077242 | MS.gene41006:CDS | 45.0% | |
| CTCCTAAGTTTGCAGTAGAG+TGG | - | chr1.3:41077263-41077282 | None:intergenic | 45.0% | |
| GATCAAGACGGAAGGAAGAT+CGG | + | chr1.3:41077835-41077854 | MS.gene41006:CDS | 45.0% | |
| GCAAACTTAGGAGCCAACTT+GGG | + | chr1.3:41077270-41077289 | MS.gene41006:CDS | 45.0% | |
| GGTCGTAGATCGACATCAAA+CGG | + | chr1.3:41077626-41077645 | MS.gene41006:intron | 45.0% | |
| TCACACGCTCCAACATCAAA+CGG | - | chr1.3:41078071-41078090 | None:intergenic | 45.0% | |
| TCCCAAACTGAACCACATGT+TGG | - | chr1.3:41077228-41077247 | None:intergenic | 45.0% | |
| TGAGTCCGAGTCTCGATTAA+AGG | - | chr1.3:41077808-41077827 | None:intergenic | 45.0% | |
| TGCAAACTTAGGAGCCAACT+TGG | + | chr1.3:41077269-41077288 | MS.gene41006:CDS | 45.0% | |
| ! | CCAACATCAAACGGTGCTTT+TGG | - | chr1.3:41078062-41078081 | None:intergenic | 45.0% |
| !! | CCAAAAGCACCGTTTGATGT+TGG | + | chr1.3:41078059-41078078 | MS.gene41006:CDS | 45.0% |
| !! | CGATCTACGACCATTGACTT+TGG | - | chr1.3:41077618-41077637 | None:intergenic | 45.0% |
| !! | TCCTCTTAGGACCCTTGTTT+TGG | - | chr1.3:41077308-41077327 | None:intergenic | 45.0% |
| AATGTGGACGCCACCTATGT+CGG | + | chr1.3:41078687-41078706 | MS.gene41006:CDS | 50.0% | |
| ACATAGGTGGCGTCCACATT+TGG | - | chr1.3:41078687-41078706 | None:intergenic | 50.0% | |
| CTACGTCGATCAACAAGCTG+AGG | + | chr1.3:41078327-41078346 | MS.gene41006:CDS | 50.0% | |
| GGAATTGGGCATGGAATGGA+TGG | + | chr1.3:41078738-41078757 | MS.gene41006:CDS | 50.0% | |
| GTTTCGTGCTCATCCGACAT+AGG | - | chr1.3:41078703-41078722 | None:intergenic | 50.0% | |
| GTTGGAGCGTGTGACGTTGA+TGG | + | chr1.3:41078077-41078096 | MS.gene41006:CDS | 55.0% | |
| TCGTGCTCATCCGACATAGG+TGG | - | chr1.3:41078700-41078719 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 41077171 | 41078795 | 41077171 | ID=MS.gene41006 |
| chr1.3 | mRNA | 41077171 | 41078795 | 41077171 | ID=MS.gene41006.t1;Parent=MS.gene41006 |
| chr1.3 | exon | 41077171 | 41077343 | 41077171 | ID=MS.gene41006.t1.exon1;Parent=MS.gene41006.t1 |
| chr1.3 | CDS | 41077171 | 41077343 | 41077171 | ID=cds.MS.gene41006.t1;Parent=MS.gene41006.t1 |
| chr1.3 | exon | 41077800 | 41077896 | 41077800 | ID=MS.gene41006.t1.exon2;Parent=MS.gene41006.t1 |
| chr1.3 | CDS | 41077800 | 41077896 | 41077800 | ID=cds.MS.gene41006.t1;Parent=MS.gene41006.t1 |
| chr1.3 | exon | 41078015 | 41078141 | 41078015 | ID=MS.gene41006.t1.exon3;Parent=MS.gene41006.t1 |
| chr1.3 | CDS | 41078015 | 41078141 | 41078015 | ID=cds.MS.gene41006.t1;Parent=MS.gene41006.t1 |
| chr1.3 | exon | 41078281 | 41078442 | 41078281 | ID=MS.gene41006.t1.exon4;Parent=MS.gene41006.t1 |
| chr1.3 | CDS | 41078281 | 41078442 | 41078281 | ID=cds.MS.gene41006.t1;Parent=MS.gene41006.t1 |
| chr1.3 | exon | 41078671 | 41078795 | 41078671 | ID=MS.gene41006.t1.exon5;Parent=MS.gene41006.t1 |
| chr1.3 | CDS | 41078671 | 41078795 | 41078671 | ID=cds.MS.gene41006.t1;Parent=MS.gene41006.t1 |
| Gene Sequence |
| Protein sequence |
