Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053491.t1 | XP_024626105.1 | 84.1 | 189 | 30 | 0 | 1 | 189 | 1 | 189 | 4.40E-70 | 274.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053491.t1 | A0A396HFV7 | 63.2 | 193 | 67 | 2 | 1 | 189 | 1 | 193 | 2.7e-45 | 191.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene053491 | 0.889155 | 3.12E-73 | -1.69E-46 |
| MS.gene053491 | MS.gene06044 | 0.920417 | 1.31E-87 | -1.69E-46 |
| MS.gene053491 | MS.gene061245 | 0.88471 | 1.52E-71 | -1.69E-46 |
| MS.gene053491 | MS.gene073742 | 0.81724 | 3.65E-52 | -1.69E-46 |
| MS.gene053491 | MS.gene41006 | 0.840923 | 6.49E-58 | -1.69E-46 |
| MS.gene053491 | MS.gene42436 | 0.847073 | 1.46E-59 | -1.69E-46 |
| MS.gene053491 | MS.gene46426 | 0.841872 | 3.65E-58 | -1.69E-46 |
| MS.gene053491 | MS.gene55278 | 0.943959 | 4.68E-103 | -1.69E-46 |
| MS.gene053491 | MS.gene66513 | 0.855167 | 7.57E-62 | -1.69E-46 |
| MS.gene053491 | MS.gene81140 | 0.813417 | 2.59E-51 | -1.69E-46 |
| MS.gene053491 | MS.gene81193 | 0.817055 | 4.02E-52 | -1.69E-46 |
| MS.gene053491 | MS.gene92171 | 0.846463 | 2.14E-59 | -1.69E-46 |
| MS.gene053491 | MS.gene93845 | 0.955972 | 8.80E-114 | -1.69E-46 |
| MS.gene053491 | MS.gene96600 | 0.84695 | 1.57E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053491.t1 | MTR_8g007240 | 33.333 | 174 | 66 | 7 | 17 | 188 | 49 | 174 | 6.28e-16 | 72.0 |
| MS.gene053491.t1 | MTR_0903s0010 | 30.055 | 183 | 72 | 6 | 14 | 187 | 42 | 177 | 3.33e-15 | 70.5 |
| MS.gene053491.t1 | MTR_3g102970 | 31.953 | 169 | 65 | 7 | 17 | 183 | 49 | 169 | 1.90e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAAATTGGAATTGAATT+TGG | 0.155038 | 4.3:-26597313 | MS.gene053491:CDS |
| AAAATCCAAAGAGGAAATTT+TGG | 0.165439 | 4.3:-26597682 | MS.gene053491:CDS |
| TCCGATTACTTAAAGCAATT+TGG | 0.183536 | 4.3:-26597349 | MS.gene053491:CDS |
| CATCTCCAAAATTTCCTCTT+TGG | 0.214318 | 4.3:+26597677 | None:intergenic |
| TTTGGAGATGTCGAAACTTT+TGG | 0.226089 | 4.3:-26597664 | MS.gene053491:CDS |
| ACCATTGTAATTTGTTCATA+TGG | 0.234426 | 4.3:-26597549 | MS.gene053491:CDS |
| GCCAAATTGCTTTAAGTAAT+CGG | 0.251128 | 4.3:+26597348 | None:intergenic |
| AATTGAATTTGGAAAAGAAT+TGG | 0.290392 | 4.3:-26597302 | MS.gene053491:CDS |
| ATTGAATTTGGAAAAGAATT+GGG | 0.336217 | 4.3:-26597301 | MS.gene053491:CDS |
| TTTGGTAAATTTCCTCCAAT+TGG | 0.356380 | 4.3:+26597752 | None:intergenic |
| AAAGAGATTGGGAAGGAAAT+TGG | 0.360942 | 4.3:-26597325 | MS.gene053491:CDS |
| AAGCAATTTGGCAAAGAGAT+TGG | 0.365860 | 4.3:-26597337 | MS.gene053491:CDS |
| CCATTGTAATTTGTTCATAT+GGG | 0.394671 | 4.3:-26597548 | MS.gene053491:CDS |
| CCCAACTACAGCCTTCTTTG+AGG | 0.420158 | 4.3:+26597393 | None:intergenic |
| AGCAATTTGGCAAAGAGATT+GGG | 0.422113 | 4.3:-26597336 | MS.gene053491:CDS |
| TGTTTGCACTTGTGTTGTCA+TGG | 0.425538 | 4.3:-26597189 | MS.gene053491:CDS |
| TCAGCTGTTGGGTATCCAAT+TGG | 0.433222 | 4.3:-26597767 | MS.gene053491:CDS |
| TTAAGCATTGCTCTAACTCT+AGG | 0.453670 | 4.3:+26597508 | None:intergenic |
| GGTAGCAAAGCTTCAGCTGT+TGG | 0.480338 | 4.3:-26597779 | MS.gene053491:CDS |
| GTAGCAAAGCTTCAGCTGTT+GGG | 0.484076 | 4.3:-26597778 | MS.gene053491:CDS |
| TATTTGCGTGAAAGTGTCTC+TGG | 0.485310 | 4.3:-26597271 | MS.gene053491:CDS |
| TGAGGACACATCATTATTTG+AGG | 0.495657 | 4.3:+26597411 | None:intergenic |
| GTTTGCACTTGTGTTGTCAT+GGG | 0.499878 | 4.3:-26597188 | MS.gene053491:CDS |
| TCCTCAAAGAAGGCTGTAGT+TGG | 0.512316 | 4.3:-26597394 | MS.gene053491:CDS |
| TAATGATGTGTCCTCAAAGA+AGG | 0.517374 | 4.3:-26597404 | MS.gene053491:CDS |
| TTCCAGTACCTTTCAAAATG+TGG | 0.526356 | 4.3:-26597434 | MS.gene053491:CDS |
| GTAATCAGATGATGAAATTG+TGG | 0.528968 | 4.3:-26597721 | MS.gene053491:CDS |
| ATCTGATTACAACCACACTT+TGG | 0.542708 | 4.3:+26597734 | None:intergenic |
| GCTGTTGGGTATCCAATTGG+AGG | 0.550598 | 4.3:-26597764 | MS.gene053491:CDS |
| CCTCAAAGAAGGCTGTAGTT+GGG | 0.553172 | 4.3:-26597393 | MS.gene053491:CDS |
| TAATCAGATGATGAAATTGT+GGG | 0.555715 | 4.3:-26597720 | MS.gene053491:CDS |
| ATTTGGAAAAGAATTGGGCA+AGG | 0.572988 | 4.3:-26597296 | MS.gene053491:CDS |
| GGAGGAAATTTACCAAAGTG+TGG | 0.573900 | 4.3:-26597746 | MS.gene053491:CDS |
| ACAACACAAGTGCAAACAGT+AGG | 0.614937 | 4.3:+26597194 | None:intergenic |
| CCCATATGAACAAATTACAA+TGG | 0.636074 | 4.3:+26597548 | None:intergenic |
| ATTGCTGCAAAGAACAGTGA+AGG | 0.636453 | 4.3:-26597481 | MS.gene053491:CDS |
| ATACAGATGAAAATCCAAAG+AGG | 0.650224 | 4.3:-26597691 | MS.gene053491:CDS |
| ATTTGGCAAAGAGATTGGGA+AGG | 0.653755 | 4.3:-26597332 | MS.gene053491:CDS |
| AAGAACAGTGAAGGTGATGG+TGG | 0.677445 | 4.3:-26597472 | MS.gene053491:CDS |
| GCAAAGAACAGTGAAGGTGA+TGG | 0.689878 | 4.3:-26597475 | MS.gene053491:CDS |
| ATTAGGGTTCCTGAAATGGG+GGG | 0.693811 | 4.3:-26597800 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTTGGAAAAGAAT+TGG | - | chr4.3:26597639-26597658 | MS.gene053491:intron | 20.0% |
| !! | ATTGAATTTGGAAAAGAATT+GGG | - | chr4.3:26597640-26597659 | MS.gene053491:intron | 20.0% |
| !!! | AAATCTGATTTGTATGTATT+TGG | - | chr4.3:26597315-26597334 | MS.gene053491:CDS | 20.0% |
| ! | AAGGAAATTGGAATTGAATT+TGG | - | chr4.3:26597628-26597647 | MS.gene053491:intron | 25.0% |
| ! | TAATCAGATGATGAAATTGT+GGG | - | chr4.3:26597221-26597240 | MS.gene053491:CDS | 25.0% |
| !! | AAAATCCAAAGAGGAAATTT+TGG | - | chr4.3:26597259-26597278 | MS.gene053491:CDS | 25.0% |
| !!! | ACCATTGTAATTTGTTCATA+TGG | - | chr4.3:26597392-26597411 | MS.gene053491:CDS | 25.0% |
| !!! | CCATTGTAATTTGTTCATAT+GGG | - | chr4.3:26597393-26597412 | MS.gene053491:CDS | 25.0% |
| ATACAGATGAAAATCCAAAG+AGG | - | chr4.3:26597250-26597269 | MS.gene053491:CDS | 30.0% | |
| CCCATATGAACAAATTACAA+TGG | + | chr4.3:26597396-26597415 | None:intergenic | 30.0% | |
| GTAATCAGATGATGAAATTG+TGG | - | chr4.3:26597220-26597239 | MS.gene053491:CDS | 30.0% | |
| TCCGATTACTTAAAGCAATT+TGG | - | chr4.3:26597592-26597611 | MS.gene053491:intron | 30.0% | |
| TTTGGTAAATTTCCTCCAAT+TGG | + | chr4.3:26597192-26597211 | None:intergenic | 30.0% | |
| ! | GCCAAATTGCTTTAAGTAAT+CGG | + | chr4.3:26597596-26597615 | None:intergenic | 30.0% |
| AAAGAGATTGGGAAGGAAAT+TGG | - | chr4.3:26597616-26597635 | MS.gene053491:intron | 35.0% | |
| AAGCAATTTGGCAAAGAGAT+TGG | - | chr4.3:26597604-26597623 | MS.gene053491:intron | 35.0% | |
| AGCAATTTGGCAAAGAGATT+GGG | - | chr4.3:26597605-26597624 | MS.gene053491:intron | 35.0% | |
| ATCTGATTACAACCACACTT+TGG | + | chr4.3:26597210-26597229 | None:intergenic | 35.0% | |
| ATTTGGAAAAGAATTGGGCA+AGG | - | chr4.3:26597645-26597664 | MS.gene053491:intron | 35.0% | |
| CATCTCCAAAATTTCCTCTT+TGG | + | chr4.3:26597267-26597286 | None:intergenic | 35.0% | |
| TAATGATGTGTCCTCAAAGA+AGG | - | chr4.3:26597537-26597556 | MS.gene053491:CDS | 35.0% | |
| TGAGGACACATCATTATTTG+AGG | + | chr4.3:26597533-26597552 | None:intergenic | 35.0% | |
| TTCCAGTACCTTTCAAAATG+TGG | - | chr4.3:26597507-26597526 | MS.gene053491:CDS | 35.0% | |
| ! | TTAAGCATTGCTCTAACTCT+AGG | + | chr4.3:26597436-26597455 | None:intergenic | 35.0% |
| ! | TTGGAGATGTCGAAACTTTT+GGG | - | chr4.3:26597278-26597297 | MS.gene053491:CDS | 35.0% |
| ! | TTTGGAGATGTCGAAACTTT+TGG | - | chr4.3:26597277-26597296 | MS.gene053491:CDS | 35.0% |
| !! | ATTTGAGGCCACATTTTGAA+AGG | + | chr4.3:26597518-26597537 | None:intergenic | 35.0% |
| ACAACACAAGTGCAAACAGT+AGG | + | chr4.3:26597750-26597769 | None:intergenic | 40.0% | |
| ATTGCTGCAAAGAACAGTGA+AGG | - | chr4.3:26597460-26597479 | MS.gene053491:CDS | 40.0% | |
| ATTTGGCAAAGAGATTGGGA+AGG | - | chr4.3:26597609-26597628 | MS.gene053491:intron | 40.0% | |
| GGAGGAAATTTACCAAAGTG+TGG | - | chr4.3:26597195-26597214 | MS.gene053491:CDS | 40.0% | |
| GTTTGCACTTGTGTTGTCAT+GGG | - | chr4.3:26597753-26597772 | MS.gene053491:CDS | 40.0% | |
| TGTTTGCACTTGTGTTGTCA+TGG | - | chr4.3:26597752-26597771 | MS.gene053491:CDS | 40.0% | |
| !! | TATTTGCGTGAAAGTGTCTC+TGG | - | chr4.3:26597670-26597689 | MS.gene053491:CDS | 40.0% |
| !!! | GATGGTGGTTTTAGCAACAT+TGG | - | chr4.3:26597484-26597503 | MS.gene053491:CDS | 40.0% |
| CCTCAAAGAAGGCTGTAGTT+GGG | - | chr4.3:26597548-26597567 | MS.gene053491:CDS | 45.0% | |
| GCAAAGAACAGTGAAGGTGA+TGG | - | chr4.3:26597466-26597485 | MS.gene053491:CDS | 45.0% | |
| GTAGCAAAGCTTCAGCTGTT+GGG | - | chr4.3:26597163-26597182 | MS.gene053491:CDS | 45.0% | |
| TCAGCTGTTGGGTATCCAAT+TGG | - | chr4.3:26597174-26597193 | MS.gene053491:CDS | 45.0% | |
| TCCTCAAAGAAGGCTGTAGT+TGG | - | chr4.3:26597547-26597566 | MS.gene053491:CDS | 45.0% | |
| ! | AAGAACAGTGAAGGTGATGG+TGG | - | chr4.3:26597469-26597488 | MS.gene053491:CDS | 45.0% |
| ! | GAGATGTCGAAACTTTTGGG+AGG | - | chr4.3:26597281-26597300 | MS.gene053491:CDS | 45.0% |
| !! | GGCCACATTTTGAAAGGTAC+TGG | + | chr4.3:26597512-26597531 | None:intergenic | 45.0% |
| CCCAACTACAGCCTTCTTTG+AGG | + | chr4.3:26597551-26597570 | None:intergenic | 50.0% | |
| GCTGTTGGGTATCCAATTGG+AGG | - | chr4.3:26597177-26597196 | MS.gene053491:CDS | 50.0% | |
| GGTAGCAAAGCTTCAGCTGT+TGG | - | chr4.3:26597162-26597181 | MS.gene053491:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 26597156 | 26597807 | 26597156 | ID=MS.gene053491 |
| chr4.3 | mRNA | 26597156 | 26597807 | 26597156 | ID=MS.gene053491.t1;Parent=MS.gene053491 |
| chr4.3 | exon | 26597661 | 26597807 | 26597661 | ID=MS.gene053491.t1.exon1;Parent=MS.gene053491.t1 |
| chr4.3 | CDS | 26597661 | 26597807 | 26597661 | ID=cds.MS.gene053491.t1;Parent=MS.gene053491.t1 |
| chr4.3 | exon | 26597156 | 26597578 | 26597156 | ID=MS.gene053491.t1.exon2;Parent=MS.gene053491.t1 |
| chr4.3 | CDS | 26597156 | 26597578 | 26597156 | ID=cds.MS.gene053491.t1;Parent=MS.gene053491.t1 |
| Gene Sequence |
| Protein sequence |
