Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41114.t1 | XP_028762943.1 | 46 | 348 | 72 | 3 | 1 | 232 | 1 | 348 | 2.40E-70 | 275.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41114.t1 | A0A1U8PVE2 | 49.8 | 269 | 84 | 2 | 1 | 218 | 1 | 269 | 9.3e-64 | 253.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene41114 | 0.804394 | 2.21E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene41114 | 0.801491 | 8.78E-49 | -1.69E-46 |
| MS.gene050561 | MS.gene41114 | 0.80985 | 1.54E-50 | -1.69E-46 |
| MS.gene050562 | MS.gene41114 | 0.804609 | 1.99E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene41114 | 0.81381 | 2.12E-51 | -1.69E-46 |
| MS.gene053998 | MS.gene41114 | 0.801628 | 8.23E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene41114 | 0.822795 | 1.96E-53 | -1.69E-46 |
| MS.gene056854 | MS.gene41114 | 0.80351 | 3.37E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene41114 | 0.812917 | 3.33E-51 | -1.69E-46 |
| MS.gene058515 | MS.gene41114 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene41114 | 0.847944 | 8.39E-60 | -1.69E-46 |
| MS.gene060167 | MS.gene41114 | 0.815089 | 1.11E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41114.t1 | MTR_7g062720 | 59.740 | 154 | 56 | 1 | 1 | 154 | 1 | 148 | 1.65e-61 | 190 |
| MS.gene41114.t1 | MTR_5g061150 | 66.372 | 113 | 38 | 0 | 20 | 132 | 10 | 122 | 2.77e-47 | 153 |
| MS.gene41114.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 152 | 232 | 5 | 80 | 9.36e-24 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTCTGTAAATAATTATC+TGG | + | 15211:8232-8251 | MS.gene41114:CDS | 15.0% |
| !! | AATGTTGTAAAGAATGATAT+TGG | + | 15211:8983-9002 | MS.gene41114:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | + | 15211:9114-9133 | MS.gene41114:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | + | 15211:8272-8291 | MS.gene41114:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | + | 15211:8273-8292 | MS.gene41114:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | + | 15211:9113-9132 | MS.gene41114:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | + | 15211:9157-9176 | MS.gene41114:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | + | 15211:9165-9184 | MS.gene41114:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | - | 15211:9085-9104 | MS.gene41114:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | - | 15211:9246-9265 | MS.gene41114:intergenic | 25.0% |
| !! | TGTAAATAATTATCTGGCTT+GGG | + | 15211:8238-8257 | MS.gene41114:CDS | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | - | 15211:8973-8992 | MS.gene41114:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | - | 15211:8273-8292 | MS.gene41114:intergenic | 25.0% |
| !! | TTTGGGACAATTTAAAAGAA+AGG | + | 15211:8426-8445 | MS.gene41114:CDS | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | - | 15211:8376-8395 | MS.gene41114:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | - | 15211:8949-8968 | MS.gene41114:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | + | 15211:8409-8428 | MS.gene41114:CDS | 30.0% | |
| ATGGTTATATTTGCGTTTAC+AGG | + | 15211:8493-8512 | MS.gene41114:CDS | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | + | 15211:8322-8341 | MS.gene41114:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | + | 15211:8296-8315 | MS.gene41114:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | + | 15211:9249-9268 | MS.gene41114:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | + | 15211:9105-9124 | MS.gene41114:CDS | 30.0% | |
| CTGTAAATAATTATCTGGCT+TGG | + | 15211:8237-8256 | MS.gene41114:CDS | 30.0% | |
| GTCTGTAATCGAATATAACT+CGG | + | 15211:8523-8542 | MS.gene41114:CDS | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | - | 15211:8627-8646 | MS.gene41114:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | + | 15211:8408-8427 | MS.gene41114:CDS | 30.0% | |
| ! | AAATTGTCCCAAAGATCTTT+TGG | - | 15211:8419-8438 | MS.gene41114:intergenic | 30.0% |
| ! | AAGATCTTTTGGATCTTCTA+TGG | - | 15211:8408-8427 | MS.gene41114:intergenic | 30.0% |
| ! | GCTTTTGATGAAAAATCACT+CGG | + | 15211:8733-8752 | MS.gene41114:intron | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | - | 15211:8373-8392 | MS.gene41114:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | + | 15211:8899-8918 | MS.gene41114:intron | 30.0% |
| !!! | ACCTTTTAAAACCCTTTTCA+CGG | - | 15211:8667-8686 | MS.gene41114:intergenic | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | - | 15211:9108-9127 | MS.gene41114:intergenic | 30.0% |
| AACCATTCATTACGAGCATT+AGG | - | 15211:8479-8498 | MS.gene41113:intergenic | 35.0% | |
| AACTGAACAACAAAAGGCTA+AGG | + | 15211:8328-8347 | MS.gene41114:CDS | 35.0% | |
| ACTTCTCTATTAACACTGTG+TGG | + | 15211:8560-8579 | MS.gene41114:CDS | 35.0% | |
| ATTAACACTGTGTGGAGAAA+AGG | + | 15211:8568-8587 | MS.gene41114:CDS | 35.0% | |
| ATTCATTACGAGCATTAGGA+AGG | - | 15211:8475-8494 | MS.gene41114:intergenic | 35.0% | |
| TTCCTAATGCTCGTAATGAA+TGG | + | 15211:8474-8493 | MS.gene41114:CDS | 35.0% | |
| ! | AGATGGTCAATCTTTTCATC+AGG | - | 15211:9229-9248 | MS.gene41114:intergenic | 35.0% |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | - | 15211:8205-8224 | MS.gene41114:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | + | 15211:8202-8221 | MS.gene41114:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | - | 15211:8403-8422 | MS.gene41114:intergenic | 35.0% |
| !!! | ACCGTGAAAAGGGTTTTAAA+AGG | + | 15211:8663-8682 | MS.gene41114:intron | 35.0% |
| AATTGTATCTCCATGCCCTT+CGG | - | 15211:8291-8310 | MS.gene41114:intergenic | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | - | 15211:9198-9217 | MS.gene41114:intergenic | 40.0% | |
| CAGCAACAATACCGTGAAAA+GGG | + | 15211:8653-8672 | MS.gene41114:intron | 40.0% | |
| CTTAATATCATGCCTGCTTG+TGG | + | 15211:8694-8713 | MS.gene41114:intron | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | - | 15211:9197-9216 | MS.gene41114:intergenic | 40.0% | |
| GATCACTATGGACAAAATCG+TGG | + | 15211:8806-8825 | MS.gene41114:intron | 40.0% | |
| GTAGCAACACATGATCACTA+TGG | + | 15211:8794-8813 | MS.gene41114:intron | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | - | 15211:8457-8476 | MS.gene41114:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | - | 15211:8784-8803 | MS.gene41114:intergenic | 40.0% |
| ! | TATTTTGTTCTGCCACAAGC+AGG | - | 15211:8709-8728 | MS.gene41114:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | - | 15211:8785-8804 | MS.gene41114:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | + | 15211:9028-9047 | MS.gene41114:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | + | 15211:9025-9044 | MS.gene41114:CDS | 40.0% |
| CATTTAAATGCCGAAGGGCA+TGG | + | 15211:8278-8297 | MS.gene41114:CDS | 45.0% | |
| GCAGCAACAATACCGTGAAA+AGG | + | 15211:8652-8671 | MS.gene41114:intron | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | + | 15211:9041-9060 | MS.gene41114:CDS | 45.0% | |
| TATGGACAAAATCGTGGTCG+TGG | + | 15211:8812-8831 | MS.gene41114:intron | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | + | 15211:9034-9053 | MS.gene41114:CDS | 45.0% | |
| TTTCACGGTATTGTTGCTGC+AGG | - | 15211:8652-8671 | MS.gene41114:intergenic | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | - | 15211:8779-8798 | MS.gene41114:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | - | 15211:8354-8373 | MS.gene41114:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | - | 15211:9074-9093 | MS.gene41114:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | + | 15211:8865-8884 | MS.gene41114:intron | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | + | 15211:8361-8380 | MS.gene41114:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | - | 15211:8364-8383 | MS.gene41114:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | + | 15211:8878-8897 | MS.gene41114:intron | 50.0% |
| CGTGGTCGTGGTTATGGAAG+GGG | + | 15211:8866-8885 | MS.gene41114:intron | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | + | 15211:8864-8883 | MS.gene41114:intron | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | - | 15211:8764-8783 | MS.gene41114:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | - | 15211:8765-8784 | MS.gene41114:intergenic | 55.0% |
| CAAAATCGTGGTCGTGGCCG+TGG | + | 15211:8818-8837 | MS.gene41114:intron | 60.0% | |
| CACGTCCACGACCATGACCA+CGG | - | 15211:8838-8857 | MS.gene41114:intergenic | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | + | 15211:8860-8879 | MS.gene41114:intron | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | + | 15211:8872-8891 | MS.gene41114:intron | 60.0% | |
| ! | AATCACTCGGCTCGTCCCAC+TGG | + | 15211:8746-8765 | MS.gene41114:intron | 60.0% |
| CATGGTCGTGGACGTGGACG+TGG | + | 15211:8842-8861 | MS.gene41114:intron | 65.0% | |
| CGTGGTCATGGTCGTGGACG+TGG | + | 15211:8836-8855 | MS.gene41114:intron | 65.0% | |
| CGTGGACGTGGACGTGGACG+TGG | + | 15211:8848-8867 | MS.gene41114:intron | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | + | 15211:8854-8873 | MS.gene41114:intron | 70.0% | |
| CGTGGCCGTGGTCATGGTCG+TGG | + | 15211:8830-8849 | MS.gene41114:intron | 70.0% | |
| CGTGGTCGTGGCCGTGGTCA+TGG | + | 15211:8824-8843 | MS.gene41114:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15211 | gene | 8194 | 9276 | 8194 | ID=MS.gene41114 |
| 15211 | mRNA | 8194 | 9276 | 8194 | ID=MS.gene41114.t1;Parent=MS.gene41114 |
| 15211 | exon | 8194 | 8589 | 8194 | ID=MS.gene41114.t1.exon1;Parent=MS.gene41114.t1 |
| 15211 | CDS | 8194 | 8589 | 8194 | ID=cds.MS.gene41114.t1;Parent=MS.gene41114.t1 |
| 15211 | exon | 8974 | 9276 | 8974 | ID=MS.gene41114.t1.exon2;Parent=MS.gene41114.t1 |
| 15211 | CDS | 8974 | 9276 | 8974 | ID=cds.MS.gene41114.t1;Parent=MS.gene41114.t1 |
| Gene Sequence |
| Protein sequence |
