AlfalfaGEDB | MS.gene41654

M. sativa cultivar XinJiangDaYe / MS.gene41654

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41654.t1	XP_013461663.1	95.7	303	11	2	1	302	1	302	5.90E-125	457.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41654.t1	Q93W88	56.2	162	55	1	138	299	127	272	1.1e-39	165.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41654.t1	A0A072V2W2	95.7	303	11	2	1	302	1	302	4.2e-125	457.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene41654.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054955	MS.gene41654	0.828666	7.92E-55	-1.69E-46
MS.gene055533	MS.gene41654	0.960817	5.48E-119	-1.69E-46
MS.gene057146	MS.gene41654	0.801318	9.53E-49	-1.69E-46
MS.gene057926	MS.gene41654	0.811923	5.49E-51	-1.69E-46
MS.gene057927	MS.gene41654	0.809107	2.23E-50	-1.69E-46
MS.gene058660	MS.gene41654	0.808634	2.81E-50	-1.69E-46
MS.gene060804	MS.gene41654	0.821274	4.40E-53	-1.69E-46
MS.gene060818	MS.gene41654	0.804882	1.75E-49	-1.69E-46
MS.gene061041	MS.gene41654	0.838711	2.44E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41654.t1	MTR_3g498825	98.020	303	4	2	1	302	1	302	0.0	606
MS.gene41654.t1	MTR_8g099880	59.032	310	94	11	1	295	1	292	3.80e-95	284
MS.gene41654.t1	MTR_1g017350	60.000	125	41	2	121	239	228	349	2.48e-43	153
MS.gene41654.t1	MTR_1g017350	60.000	125	41	2	121	239	228	349	3.33e-43	153
MS.gene41654.t1	MTR_3g116770	63.200	125	30	1	111	219	139	263	7.27e-43	152
MS.gene41654.t1	MTR_3g116770	63.200	125	30	1	111	219	139	263	7.36e-43	152
MS.gene41654.t1	MTR_7g053410	57.516	153	45	3	86	218	236	388	1.29e-42	153
MS.gene41654.t1	MTR_1g017350	61.667	120	37	2	121	234	228	344	1.69e-42	151
MS.gene41654.t1	MTR_8g065740	69.091	110	31	1	113	219	268	377	1.93e-42	152
MS.gene41654.t1	MTR_7g053410	57.516	153	45	3	86	218	236	388	2.08e-42	153
MS.gene41654.t1	MTR_8g012290	62.500	136	40	4	109	236	245	377	2.15e-42	152
MS.gene41654.t1	MTR_8g012290	62.500	136	40	4	109	236	245	377	2.16e-42	152
MS.gene41654.t1	MTR_1g017350	61.667	120	37	2	121	234	228	344	2.26e-42	151
MS.gene41654.t1	MTR_5g037250	61.475	122	45	2	98	218	63	183	3.22e-42	145
MS.gene41654.t1	MTR_5g048860	56.738	141	49	2	91	219	297	437	4.17e-41	150
MS.gene41654.t1	MTR_4g070320	70.642	109	28	3	113	219	109	215	2.28e-40	144
MS.gene41654.t1	MTR_8g062240	86.667	75	10	0	144	218	148	222	8.40e-40	140
MS.gene41654.t1	MTR_1g059270	55.652	115	42	2	144	252	157	268	2.31e-36	133
MS.gene41654.t1	MTR_1g059270	65.217	92	29	1	144	235	157	245	2.81e-36	130
MS.gene41654.t1	MTR_7g092510	68.889	90	25	1	144	233	170	256	2.00e-35	130
MS.gene41654.t1	MTR_6g084120	50.000	124	33	5	112	231	134	232	6.10e-27	106
MS.gene41654.t1	MTR_4g131160	60.000	85	29	2	147	227	196	279	2.27e-23	99.4
MS.gene41654.t1	MTR_4g131160	60.000	85	29	2	147	227	199	282	2.63e-23	99.0
MS.gene41654.t1	MTR_1g080890	61.039	77	29	1	136	212	182	257	1.52e-21	93.6
MS.gene41654.t1	MTR_4g033295	60.000	65	26	0	147	211	118	182	6.35e-18	80.5
MS.gene41654.t1	MTR_4g033295	58.209	67	27	1	147	212	118	184	8.99e-18	79.7
MS.gene41654.t1	MTR_4g065870	61.538	65	25	0	147	211	355	419	5.25e-17	81.3
MS.gene41654.t1	MTR_4g065870	61.290	62	24	0	147	208	355	416	2.74e-16	79.0
MS.gene41654.t1	MTR_1g069155	40.952	105	60	2	118	221	287	390	4.63e-16	78.6
MS.gene41654.t1	MTR_1g052470	50.633	79	39	0	147	225	298	376	6.55e-16	77.8
MS.gene41654.t1	MTR_2g098310	56.923	65	28	0	147	211	332	396	1.37e-15	77.0
MS.gene41654.t1	MTR_1g070870	40.909	132	72	2	110	236	78	208	2.10e-15	75.1
MS.gene41654.t1	MTR_7g092540	53.731	67	31	0	147	213	243	309	4.36e-15	74.7
MS.gene41654.t1	MTR_2g098310	56.061	66	29	0	145	210	330	395	4.51e-15	75.5
MS.gene41654.t1	MTR_1g070870	48.980	98	49	1	139	236	115	211	6.40e-15	73.9
MS.gene41654.t1	MTR_1g084980	37.273	110	64	3	107	212	418	526	8.06e-15	75.1
MS.gene41654.t1	MTR_7g092540	54.688	64	29	0	147	210	243	306	8.54e-15	73.9
MS.gene41654.t1	MTR_3g028520	53.846	65	30	0	147	211	322	386	3.97e-14	72.8
MS.gene41654.t1	MTR_3g027650	53.846	65	30	0	147	211	322	386	3.97e-14	72.8
MS.gene41654.t1	MTR_3g027650	54.839	62	28	0	147	208	322	383	1.68e-13	70.9
MS.gene41654.t1	MTR_7g099540	48.611	72	36	1	150	221	255	325	2.96e-13	70.1
MS.gene41654.t1	MTR_5g017040	37.391	115	67	3	112	222	67	180	1.44e-12	67.8
MS.gene41654.t1	MTR_3g449770	47.945	73	36	2	149	221	311	381	2.45e-12	67.4
MS.gene41654.t1	MTR_8g036235	49.231	65	33	0	147	211	316	380	2.54e-12	67.0
MS.gene41654.t1	MTR_1g019240	39.130	92	54	2	149	239	87	177	6.42e-12	65.1
MS.gene41654.t1	MTR_1g018090	53.125	64	29	1	148	211	275	337	8.68e-12	65.5
MS.gene41654.t1	MTR_4g088710	96.552	29	1	0	275	303	9	37	1.31e-11	59.7
MS.gene41654.t1	MTR_1g116790	50.000	64	32	0	147	210	265	328	1.67e-11	64.3
MS.gene41654.t1	MTR_2g098310	43.750	96	46	3	147	235	332	426	3.39e-11	63.9
MS.gene41654.t1	MTR_7g099540	44.872	78	36	2	150	221	255	331	4.65e-11	63.5
MS.gene41654.t1	MTR_3g103030	48.052	77	38	2	148	223	230	305	4.67e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41654.t1	AT5G50915	48.069	233	97	6	74	299	57	272	4.03e-59	191
MS.gene41654.t1	AT5G50915	48.069	233	97	6	74	299	57	272	4.03e-59	191
MS.gene41654.t1	AT5G50915	50.228	219	82	6	74	286	57	254	5.12e-59	190
MS.gene41654.t1	AT5G50915	50.228	219	82	6	74	286	57	254	5.12e-59	190
MS.gene41654.t1	AT1G68920	57.718	149	59	2	86	234	243	387	1.16e-46	164
MS.gene41654.t1	AT1G68920	57.718	149	59	2	86	234	243	387	1.16e-46	164
MS.gene41654.t1	AT1G68920	57.718	149	59	2	86	234	243	387	1.16e-46	164
MS.gene41654.t1	AT1G68920	56.209	153	54	2	92	234	237	386	1.37e-46	163
MS.gene41654.t1	AT1G59640	68.966	116	31	2	108	218	92	207	9.22e-45	153
MS.gene41654.t1	AT1G10120	60.976	123	45	1	112	234	171	290	1.04e-44	156
MS.gene41654.t1	AT1G10120	60.976	123	45	1	112	234	171	290	1.04e-44	156
MS.gene41654.t1	AT3G07340	65.079	126	43	1	93	218	205	329	2.97e-44	156
MS.gene41654.t1	AT1G26260	52.353	170	49	4	94	234	90	256	3.62e-44	154
MS.gene41654.t1	AT1G26260	52.353	170	49	4	94	234	119	285	4.12e-44	154
MS.gene41654.t1	AT3G07340	65.079	126	43	1	93	218	205	329	5.10e-44	156
MS.gene41654.t1	AT1G26260	52.353	170	49	4	94	234	141	307	7.21e-44	154
MS.gene41654.t1	AT1G26260	52.353	170	49	4	94	234	141	307	7.21e-44	154
MS.gene41654.t1	AT1G26260	52.353	170	49	4	94	234	141	307	7.21e-44	154
MS.gene41654.t1	AT1G26260	52.353	170	49	4	94	234	141	307	7.21e-44	154
MS.gene41654.t1	AT1G59640	68.966	116	31	2	108	218	92	207	1.51e-43	152
MS.gene41654.t1	AT5G48560	83.146	89	15	0	139	227	293	381	1.94e-43	155
MS.gene41654.t1	AT5G48560	83.146	89	15	0	139	227	293	381	3.76e-43	154
MS.gene41654.t1	AT3G23690	60.000	135	41	4	112	234	146	279	5.45e-42	149
MS.gene41654.t1	AT4G34530	87.179	78	10	0	142	219	167	244	3.33e-41	146
MS.gene41654.t1	AT5G62610	71.910	89	25	0	130	218	136	224	7.32e-39	138
MS.gene41654.t1	AT5G62610	71.910	89	25	0	130	218	136	224	7.32e-39	138
MS.gene41654.t1	AT4G36540	57.143	133	46	3	111	235	97	226	2.56e-38	137
MS.gene41654.t1	AT4G36540	57.143	133	46	3	111	235	97	226	2.88e-38	137
MS.gene41654.t1	AT2G18300	49.057	159	70	4	85	243	131	278	6.30e-38	137
MS.gene41654.t1	AT2G18300	55.556	135	52	3	85	219	131	257	1.34e-37	136
MS.gene41654.t1	AT2G18300	55.556	135	52	3	85	219	131	257	2.65e-37	136
MS.gene41654.t1	AT2G42300	64.583	96	31	1	144	239	182	274	6.99e-37	133
MS.gene41654.t1	AT1G18400	71.264	87	24	1	145	231	143	228	7.06e-37	132
MS.gene41654.t1	AT3G57800	71.111	90	23	1	144	233	201	287	9.68e-37	135
MS.gene41654.t1	AT1G18400	71.264	87	24	1	145	231	143	228	1.13e-36	132
MS.gene41654.t1	AT1G73830	68.478	92	28	1	146	237	146	236	4.38e-36	130
MS.gene41654.t1	AT1G73830	70.930	86	24	1	146	231	146	230	8.47e-36	130
MS.gene41654.t1	AT2G42300	66.304	92	28	1	144	235	182	270	1.30e-35	131
MS.gene41654.t1	AT1G25330	52.518	139	54	4	99	231	51	183	9.64e-34	123
MS.gene41654.t1	AT3G57800	46.763	139	24	2	144	235	201	336	6.02e-28	112
MS.gene41654.t1	AT2G24260	65.753	73	24	1	140	212	28	99	1.44e-23	97.1
MS.gene41654.t1	AT2G24260	65.753	73	24	1	140	212	131	202	1.13e-22	96.7
MS.gene41654.t1	AT5G58010	52.941	102	37	3	147	244	99	193	2.23e-22	95.1
MS.gene41654.t1	AT4G30980	71.212	66	18	1	147	212	130	194	3.39e-22	94.7
MS.gene41654.t1	AT4G30980	71.212	66	18	1	147	212	178	242	4.67e-22	95.1
MS.gene41654.t1	AT2G42300	83.721	43	7	0	144	186	182	224	6.24e-19	84.0
MS.gene41654.t1	AT2G42300	83.721	43	7	0	144	186	182	224	6.24e-19	84.0
MS.gene41654.t1	AT2G42300	83.721	43	7	0	144	186	182	224	7.55e-19	84.0
MS.gene41654.t1	AT4G09180	53.731	67	31	0	145	211	182	248	3.22e-15	74.3
MS.gene41654.t1	AT1G35460	53.846	65	30	0	147	211	181	245	3.48e-15	74.3
MS.gene41654.t1	AT2G42280	54.688	64	29	0	147	210	279	342	5.43e-15	75.1
MS.gene41654.t1	AT1G51140	57.812	64	27	0	147	210	304	367	8.03e-15	74.7
MS.gene41654.t1	AT2G42280	56.061	66	27	1	147	210	279	344	9.03e-15	74.3
MS.gene41654.t1	AT4G28790	47.297	74	38	1	149	222	273	345	1.72e-14	73.9
MS.gene41654.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene41654.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene41654.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene41654.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene41654.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene41654.t1	AT1G09530	43.820	89	46	2	124	212	317	401	1.93e-14	73.9
MS.gene41654.t1	AT4G02590	40.310	129	63	4	109	224	36	163	4.59e-14	70.9
MS.gene41654.t1	AT4G02590	40.310	129	63	4	109	224	99	226	4.69e-14	71.6
MS.gene41654.t1	AT4G02590	40.310	129	63	4	109	224	99	226	4.69e-14	71.6
MS.gene41654.t1	AT4G28800	48.611	72	36	1	150	221	252	322	4.78e-14	72.4
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	210	271	6.78e-14	72.0
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	210	271	6.78e-14	72.0
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	210	271	6.78e-14	72.0
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	281	342	7.28e-14	72.0
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	281	342	7.28e-14	72.0
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	281	342	7.28e-14	72.0
MS.gene41654.t1	AT2G20180	52.381	63	29	1	150	212	281	342	7.28e-14	72.0
MS.gene41654.t1	AT4G28815	50.000	70	33	2	150	218	71	139	7.49e-14	70.1
MS.gene41654.t1	AT4G28815	50.000	70	33	2	150	218	147	215	2.40e-13	69.7
MS.gene41654.t1	AT1G03040	53.659	82	33	2	147	224	139	219	3.74e-13	68.9
MS.gene41654.t1	AT1G03040	53.659	82	33	2	147	224	144	224	4.37e-13	68.9
MS.gene41654.t1	AT2G43140	53.125	64	30	0	147	210	134	197	5.91e-13	67.0
MS.gene41654.t1	AT2G43140	53.125	64	30	0	147	210	231	294	7.00e-13	68.2
MS.gene41654.t1	AT1G03040	53.659	82	33	2	147	224	144	224	7.13e-13	68.6
MS.gene41654.t1	AT4G28800	52.459	61	28	1	150	210	264	323	1.33e-12	68.2
MS.gene41654.t1	AT2G20180	51.667	60	28	1	150	209	281	339	1.88e-12	67.4
MS.gene41654.t1	AT1G27740	36.170	141	72	5	86	223	118	243	2.99e-12	65.9
MS.gene41654.t1	AT4G00050	49.275	69	34	1	143	211	101	168	1.32e-11	64.3
MS.gene41654.t1	AT1G05805	48.438	64	33	0	147	210	283	346	2.22e-11	64.3
MS.gene41654.t1	AT4G00050	49.275	69	34	1	143	211	203	270	2.27e-11	64.3
MS.gene41654.t1	AT4G00050	49.275	69	34	1	143	211	203	270	2.92e-11	63.9
MS.gene41654.t1	AT4G28790	45.455	66	35	1	149	214	273	337	4.58e-11	63.2
MS.gene41654.t1	AT2G14760	45.349	86	42	3	147	228	237	321	4.75e-11	63.2
MS.gene41654.t1	AT4G33880	50.769	65	31	1	147	211	266	329	9.54e-11	62.4

Find 63 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAGGGTTAGTATTGTTTC+TGG	0.191984	3.4:-88615912	None:intergenic
TCCACAGTCTTCCATGGTTC+TGG	0.296713	3.4:+88615823	MS.gene41654:CDS
GCAAAAGTTGTGCCTTGTTT+AGG	0.309108	3.4:-88617168	None:intergenic
CTCAGTTATGGTAGTAACTT+TGG	0.320242	3.4:-88615768	None:intergenic
AGCTGCTAATGACTACCTTT+TGG	0.330118	3.4:+88617227	MS.gene41654:CDS
ATGGATTGTAACCAGGTAAC+TGG	0.332974	3.4:+88616751	MS.gene41654:intron
AAAAGATCAGAGAAAGTGTT+TGG	0.372452	3.4:+88616323	MS.gene41654:CDS
GGACGTGGATATAGCCAGTT+GGG	0.373564	3.4:-88616353	None:intergenic
AACTGTGAAGGTGGAGGAGG+AGG	0.387298	3.4:-88615698	None:intergenic
AACAGACCTAGACACTCTTT+TGG	0.397586	3.4:+88616982	MS.gene41654:CDS
GTTCTGGACAAGCTTGAAAA+TGG	0.416319	3.4:+88615839	MS.gene41654:CDS
TGAAACTGTGAAGGTGGAGG+AGG	0.419275	3.4:-88615701	None:intergenic
AGAAGGTTCATTCTCAGTTA+TGG	0.434337	3.4:-88615780	None:intergenic
TTATCAGAAATTAAATAACT+TGG	0.449559	3.4:+88617104	MS.gene41654:intron
TGGCCTGACCCCTTCTTGCT+CGG	0.454614	3.4:-88616374	None:intergenic
TAGCCACAGCCTTGCTGAAA+GGG	0.456249	3.4:+88616401	MS.gene41654:CDS
GACCCCTTCTTGCTCGGACG+TGG	0.465774	3.4:-88616368	None:intergenic
CGTCCGAGCAAGAAGGGGTC+AGG	0.472206	3.4:+88616371	MS.gene41654:CDS
GAAGATGTTGGTTGGAGTCA+TGG	0.483883	3.4:-88617202	None:intergenic
GAAGAGAGAAAATAAGTGAA+AGG	0.496748	3.4:+88616564	MS.gene41654:CDS
GACACAGATGATAATGTTGA+TGG	0.508396	3.4:-88617132	None:intergenic
ATTAGCAGCTTGGAAGATGT+TGG	0.513901	3.4:-88617214	None:intergenic
AATATTATTACATGAGAGTG+AGG	0.517794	3.4:-88615929	None:intergenic
TGACAGGATAATGCAGCAGA+AGG	0.520312	3.4:+88616246	MS.gene41654:intron
AAGTTGCAGCAACTTGTGCC+AGG	0.526665	3.4:+88616593	MS.gene41654:CDS
GGGTGAAACTGTGAAGGTGG+AGG	0.526667	3.4:-88615704	None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG	0.530497	3.4:+88616400	MS.gene41654:CDS
ACTTGTGCCAGGCTGTGATA+AGG	0.531492	3.4:+88616604	MS.gene41654:CDS
AAAGGTAGTCATTAGCAGCT+TGG	0.531755	3.4:-88617224	None:intergenic
CCATGGAAGACTGTGGACTA+AGG	0.533292	3.4:-88615817	None:intergenic
CCTTAGTCCACAGTCTTCCA+TGG	0.535388	3.4:+88615817	MS.gene41654:CDS
GCAGCTTGGAAGATGTTGGT+TGG	0.538644	3.4:-88617210	None:intergenic
TTAATAATATCTTGATGAAG+AGG	0.545994	3.4:-88615725	None:intergenic
AGCAAGGCTGTGGCTATCAG+TGG	0.554257	3.4:-88616394	None:intergenic
TATCCACGTCCGAGCAAGAA+GGG	0.556525	3.4:+88616365	MS.gene41654:CDS
TTGAAGCTGAAGTATCCAAA+AGG	0.556829	3.4:-88617242	None:intergenic
GTTCTCCTCTTCTTCTCCGT+AGG	0.559768	3.4:-88615890	None:intergenic
CGGACGTGGATATAGCCAGT+TGG	0.559837	3.4:-88616354	None:intergenic
ATAGTCCTACGGAGAAGAAG+AGG	0.562653	3.4:+88615885	MS.gene41654:CDS
AATGATGAGTCAACAAGGAA+AGG	0.563177	3.4:-88615650	None:intergenic
ATGAGTCAACAAGGAAAGGA+TGG	0.563812	3.4:-88615646	None:intergenic
ATACCCTTTCAGCAAGGCTG+TGG	0.567250	3.4:-88616404	None:intergenic
TGACCTACCTTATCACAGCC+TGG	0.577941	3.4:-88616611	None:intergenic
GTCTAACCAAAAGAGTGTCT+AGG	0.588703	3.4:-88616988	None:intergenic
TTCAAGCTTGTCCAGAACCA+TGG	0.592122	3.4:-88615834	None:intergenic
GTTGCCATGTCATAGAACAT+AGG	0.595363	3.4:-88616962	None:intergenic
ATATCCACGTCCGAGCAAGA+AGG	0.602822	3.4:+88616364	MS.gene41654:CDS
AGAGGGTGAAACTGTGAAGG+TGG	0.604355	3.4:-88615707	None:intergenic
GTCTTTACAGAATCAAGTAG+AGG	0.604540	3.4:+88616813	MS.gene41654:CDS
GTCATCAAATGTTGTCTCTG+AGG	0.606377	3.4:+88617284	MS.gene41654:CDS
TTGTAACCAGGTAACTGGAA+AGG	0.609989	3.4:+88616756	MS.gene41654:intron
ATATTATTACATGAGAGTGA+GGG	0.611838	3.4:-88615928	None:intergenic
TAATAATATCTTGATGAAGA+GGG	0.621927	3.4:-88615724	None:intergenic
GACGTGGATATAGCCAGTTG+GGG	0.624086	3.4:-88616352	None:intergenic
GACAGGATAATGCAGCAGAA+GGG	0.624145	3.4:+88616247	MS.gene41654:intron
ATCCACGTCCGAGCAAGAAG+GGG	0.635705	3.4:+88616366	MS.gene41654:CDS
TCCAGAACCATGGAAGACTG+TGG	0.648687	3.4:-88615824	None:intergenic
GCATGAATGATGAGTCAACA+AGG	0.650211	3.4:-88615655	None:intergenic
CATTGCAATAGTCCTAAACA+AGG	0.657273	3.4:+88617156	MS.gene41654:CDS
TGTACTATACCCTTTCAGCA+AGG	0.661128	3.4:-88616410	None:intergenic
TGAAGAGGGTGAAACTGTGA+AGG	0.663457	3.4:-88615710	None:intergenic
GAATCCTATGTTCTATGACA+TGG	0.666100	3.4:+88616958	MS.gene41654:CDS
ACGTGGATATAGCCAGTTGG+GGG	0.714907	3.4:-88616351	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAATTTACTTAATAATACT+TGG	+	chr3.4:88617558-88617577	MS.gene41654:intron	10.0%
!!!	TTATAATTTTTTTTTTCTTC+AGG	+	chr3.4:88617597-88617616	MS.gene41654:intron	10.0%
!!	AAAATGATTTAATCCTAAAA+TGG	+	chr3.4:88617329-88617348	MS.gene41654:intron	15.0%
!!	AATTATATAATATAAGAGAC+AGG	-	chr3.4:88617539-88617558	None:intergenic	15.0%
!!	TTATCAGAAATTAAATAACT+TGG	+	chr3.4:88617104-88617123	MS.gene41654:intron	15.0%
!!!	GTGAATTTTAAATTAATGAA+AGG	-	chr3.4:88617467-88617486	None:intergenic	15.0%
!!!	TTAGGATTAAATCATTTTTT+TGG	-	chr3.4:88617327-88617346	None:intergenic	15.0%
!!	AAATTGGATGAATTATATTC+AGG	+	chr3.4:88616538-88616557	MS.gene41654:intron	20.0%
!!	TAATAATATCTTGATGAAGA+GGG	-	chr3.4:88615727-88615746	None:intergenic	20.0%
!!	TGTTAGTAATTAAATATGAC+AGG	+	chr3.4:88616230-88616249	MS.gene41654:intron	20.0%
!!	TGTTGCAAATTGAATTAAAT+TGG	+	chr3.4:88616522-88616541	MS.gene41654:intron	20.0%
!!	TTAATAATATCTTGATGAAG+AGG	-	chr3.4:88615728-88615747	None:intergenic	20.0%
!!!	AAAAAATAACATTTTCAGGA+TGG	+	chr3.4:88617379-88617398	MS.gene41654:intron	20.0%
!!!	AAACTTGTGGATAATTTTTT+TGG	+	chr3.4:88616091-88616110	MS.gene41654:intron	20.0%
!!!	AGAATAATTACATCCATTTT+AGG	-	chr3.4:88617345-88617364	None:intergenic	20.0%
!!!	TGACAAAAAATAACATTTTC+AGG	+	chr3.4:88617375-88617394	MS.gene41654:intron	20.0%
!!!	TTAGCACTTTTTTTATACTA+TGG	+	chr3.4:88616124-88616143	MS.gene41654:intron	20.0%
!	AATATTATTACATGAGAGTG+AGG	-	chr3.4:88615932-88615951	None:intergenic	25.0%
!	AGACATGAAGATAACTAAAA+GGG	-	chr3.4:88616493-88616512	None:intergenic	25.0%
!	ATATTATTACATGAGAGTGA+GGG	-	chr3.4:88615931-88615950	None:intergenic	25.0%
!	TAATCAACTCAATTCATAAC+AGG	-	chr3.4:88616196-88616215	None:intergenic	25.0%
!	TATATGATGATTCACATGTT+AGG	+	chr3.4:88617037-88617056	MS.gene41654:intron	25.0%
!!	ACTAGTATTTTCATTTCATG+TGG	+	chr3.4:88616435-88616454	MS.gene41654:intron	25.0%
!!	ATAACTAAAAGGGACATTTT+TGG	-	chr3.4:88616483-88616502	None:intergenic	25.0%
!!	ATTATTTATGGATTGTAACC+AGG	+	chr3.4:88616744-88616763	MS.gene41654:intron	25.0%
!!	CCACAAGTTTTCTTAAAATA+AGG	-	chr3.4:88616081-88616100	None:intergenic	25.0%
!!	CTAGTATTTTCATTTCATGT+GGG	+	chr3.4:88616436-88616455	MS.gene41654:intron	25.0%
!!	TAGTATTTTCATTTCATGTG+GGG	+	chr3.4:88616437-88616456	MS.gene41654:intron	25.0%
!!!	CCTTATTTTAAGAAAACTTG+TGG	+	chr3.4:88616078-88616097	MS.gene41654:intron	25.0%
!!!	TTTGAGACTTTTAATTGCAT+GGG	-	chr3.4:88616666-88616685	None:intergenic	25.0%
!!!	TTTTGAGACTTTTAATTGCA+TGG	-	chr3.4:88616667-88616686	None:intergenic	25.0%
	ATCTCTAATTCTTCTGTCAT+AGG	-	chr3.4:88616044-88616063	None:intergenic	30.0%
	CAAAAGTTTCTTCATTCACA+TGG	+	chr3.4:88617421-88617440	MS.gene41654:intron	30.0%
	GAAAAACAAAGAAGATGAGA+AGG	+	chr3.4:88616287-88616306	MS.gene41654:CDS	30.0%
	GAAGAGAGAAAATAAGTGAA+AGG	+	chr3.4:88616564-88616583	MS.gene41654:CDS	30.0%
	GAGACATGAAGATAACTAAA+AGG	-	chr3.4:88616494-88616513	None:intergenic	30.0%
	TACTCAGAAAAATAGTCCTA+CGG	+	chr3.4:88615874-88615893	MS.gene41654:CDS	30.0%
	TCTAGCAGTGATATATATGA+TGG	-	chr3.4:88615975-88615994	None:intergenic	30.0%
	TTGGAATTGTATCATACAAG+TGG	-	chr3.4:88616464-88616483	None:intergenic	30.0%
!	AGTGATATATATGATGGAGA+TGG	-	chr3.4:88615969-88615988	None:intergenic	30.0%
!	CAGCTTCAATTTTTCTACAA+GGG	+	chr3.4:88617256-88617275	MS.gene41654:CDS	30.0%
!	TCAGCTTCAATTTTTCTACA+AGG	+	chr3.4:88617255-88617274	MS.gene41654:CDS	30.0%
!!	AAAAGATCAGAGAAAGTGTT+TGG	+	chr3.4:88616323-88616342	MS.gene41654:CDS	30.0%
!!	AATATTCTCAAGCTGTTTTG+TGG	+	chr3.4:88616843-88616862	MS.gene41654:intron	30.0%
!!!	AAACATGTTGGTAGTTTTGT+AGG	-	chr3.4:88617502-88617521	None:intergenic	30.0%
!!!	AACATGTTGGTAGTTTTGTA+GGG	-	chr3.4:88617501-88617520	None:intergenic	30.0%
	AATGATGAGTCAACAAGGAA+AGG	-	chr3.4:88615653-88615672	None:intergenic	35.0%
	AGAAGGTTCATTCTCAGTTA+TGG	-	chr3.4:88615783-88615802	None:intergenic	35.0%
	CATTGCAATAGTCCTAAACA+AGG	+	chr3.4:88617156-88617175	MS.gene41654:CDS	35.0%
	CTCAGTTATGGTAGTAACTT+TGG	-	chr3.4:88615771-88615790	None:intergenic	35.0%
	GAATCCTATGTTCTATGACA+TGG	+	chr3.4:88616958-88616977	MS.gene41654:CDS	35.0%
	GTCTTTACAGAATCAAGTAG+AGG	+	chr3.4:88616813-88616832	MS.gene41654:CDS	35.0%
	TTGAGAGCTTACAAACATGT+TGG	-	chr3.4:88617514-88617533	None:intergenic	35.0%
!	CAATTTTTCTACAAGGGCAA+AGG	+	chr3.4:88617262-88617281	MS.gene41654:CDS	35.0%
!	GACACAGATGATAATGTTGA+TGG	-	chr3.4:88617135-88617154	None:intergenic	35.0%
!	TTGAAGCTGAAGTATCCAAA+AGG	-	chr3.4:88617245-88617264	None:intergenic	35.0%
!!	TTCAGGATGGTAGTCATTTT+TGG	+	chr3.4:88617392-88617411	MS.gene41654:intron	35.0%
!!!	AGGTTTTTGGTGAAAGAAGA+AGG	-	chr3.4:88615800-88615819	None:intergenic	35.0%
!!!	TCAGGATGGTAGTCATTTTT+GGG	+	chr3.4:88617393-88617412	MS.gene41654:intron	35.0%
	AAAGGTAGTCATTAGCAGCT+TGG	-	chr3.4:88617227-88617246	None:intergenic	40.0%
	ACCTTCATGACAGCACATAT+AGG	+	chr3.4:88616640-88616659	MS.gene41654:intron	40.0%
	ATGAGTCAACAAGGAAAGGA+TGG	-	chr3.4:88615649-88615668	None:intergenic	40.0%
	ATTAGCAGCTTGGAAGATGT+TGG	-	chr3.4:88617217-88617236	None:intergenic	40.0%
	GCATGAATGATGAGTCAACA+AGG	-	chr3.4:88615658-88615677	None:intergenic	40.0%
	GTCATCAAATGTTGTCTCTG+AGG	+	chr3.4:88617284-88617303	MS.gene41654:CDS	40.0%
	GTTGCCATGTCATAGAACAT+AGG	-	chr3.4:88616965-88616984	None:intergenic	40.0%
	TGTACTATACCCTTTCAGCA+AGG	-	chr3.4:88616413-88616432	None:intergenic	40.0%
	TTGTAACCAGGTAACTGGAA+AGG	+	chr3.4:88616756-88616775	MS.gene41654:intron	40.0%
!	AACAGACCTAGACACTCTTT+TGG	+	chr3.4:88616982-88617001	MS.gene41654:CDS	40.0%
!	AGCTGCTAATGACTACCTTT+TGG	+	chr3.4:88617227-88617246	MS.gene41654:CDS	40.0%
!	ATGGATTGTAACCAGGTAAC+TGG	+	chr3.4:88616751-88616770	MS.gene41654:intron	40.0%
!	GTGAGGGTTAGTATTGTTTC+TGG	-	chr3.4:88615915-88615934	None:intergenic	40.0%
!	GTTCTGGACAAGCTTGAAAA+TGG	+	chr3.4:88615839-88615858	MS.gene41654:CDS	40.0%
!!	AGACTGTGGACTAAGGTTTT+TGG	-	chr3.4:88615813-88615832	None:intergenic	40.0%
!!	CACTCTTTTGGTTAGACAAG+AGG	+	chr3.4:88616994-88617013	MS.gene41654:CDS	40.0%
!!	GCAAAAGTTGTGCCTTGTTT+AGG	-	chr3.4:88617171-88617190	None:intergenic	40.0%
!!	GTCTAACCAAAAGAGTGTCT+AGG	-	chr3.4:88616991-88617010	None:intergenic	40.0%
!!!	GATGGTAGTCATTTTTGGGA+GGG	+	chr3.4:88617397-88617416	MS.gene41654:intron	40.0%
	ATAGTCCTACGGAGAAGAAG+AGG	+	chr3.4:88615885-88615904	MS.gene41654:CDS	45.0%
	GACAGGATAATGCAGCAGAA+GGG	+	chr3.4:88616247-88616266	MS.gene41654:intron	45.0%
	GCCTATATGTGCTGTCATGA+AGG	-	chr3.4:88616644-88616663	None:intergenic	45.0%
	TAAGAGCCTTTCCAGTTACC+TGG	-	chr3.4:88616765-88616784	None:intergenic	45.0%
	TGAAGAGGGTGAAACTGTGA+AGG	-	chr3.4:88615713-88615732	None:intergenic	45.0%
	TGACAGGATAATGCAGCAGA+AGG	+	chr3.4:88616246-88616265	MS.gene41654:intron	45.0%
	TTCAAGCTTGTCCAGAACCA+TGG	-	chr3.4:88615837-88615856	None:intergenic	45.0%
!	GAAGATGTTGGTTGGAGTCA+TGG	-	chr3.4:88617205-88617224	None:intergenic	45.0%
!!!	GGATGGTAGTCATTTTTGGG+AGG	+	chr3.4:88617396-88617415	MS.gene41654:intron	45.0%
!!	TCATAAAATATAATTATTTA+TGG	+	chr3.4:88616732-88616751	MS.gene41654:intron	5.0%
	AAGTTGCAGCAACTTGTGCC+AGG	+	chr3.4:88616593-88616612	MS.gene41654:CDS	50.0%
	ACGTGGATATAGCCAGTTGG+GGG	-	chr3.4:88616354-88616373	None:intergenic	50.0%
	ACTTGTGCCAGGCTGTGATA+AGG	+	chr3.4:88616604-88616623	MS.gene41654:CDS	50.0%
	AGAGGGTGAAACTGTGAAGG+TGG	-	chr3.4:88615710-88615729	None:intergenic	50.0%
	ATACCCTTTCAGCAAGGCTG+TGG	-	chr3.4:88616407-88616426	None:intergenic	50.0%
	ATAGCCACAGCCTTGCTGAA+AGG	+	chr3.4:88616400-88616419	MS.gene41654:CDS	50.0%
	ATATCCACGTCCGAGCAAGA+AGG	+	chr3.4:88616364-88616383	MS.gene41654:CDS	50.0%
	CATGTGTGTGTGTGTGTGCA+TGG	-	chr3.4:88616164-88616183	None:intergenic	50.0%
	CCTTAGTCCACAGTCTTCCA+TGG	+	chr3.4:88615817-88615836	MS.gene41654:CDS	50.0%
	GACGTGGATATAGCCAGTTG+GGG	-	chr3.4:88616355-88616374	None:intergenic	50.0%
	GCAGCTTGGAAGATGTTGGT+TGG	-	chr3.4:88617213-88617232	None:intergenic	50.0%
	GGACGTGGATATAGCCAGTT+GGG	-	chr3.4:88616356-88616375	None:intergenic	50.0%
	GTTCTCCTCTTCTTCTCCGT+AGG	-	chr3.4:88615893-88615912	None:intergenic	50.0%
	TAGCCACAGCCTTGCTGAAA+GGG	+	chr3.4:88616401-88616420	MS.gene41654:CDS	50.0%
	TATCCACGTCCGAGCAAGAA+GGG	+	chr3.4:88616365-88616384	MS.gene41654:CDS	50.0%
	TCCACAGTCTTCCATGGTTC+TGG	+	chr3.4:88615823-88615842	MS.gene41654:CDS	50.0%
	TCCAGAACCATGGAAGACTG+TGG	-	chr3.4:88615827-88615846	None:intergenic	50.0%
	TGAAACTGTGAAGGTGGAGG+AGG	-	chr3.4:88615704-88615723	None:intergenic	50.0%
	TGACCTACCTTATCACAGCC+TGG	-	chr3.4:88616614-88616633	None:intergenic	50.0%
!	CCATGGAAGACTGTGGACTA+AGG	-	chr3.4:88615820-88615839	None:intergenic	50.0%
	AACTGTGAAGGTGGAGGAGG+AGG	-	chr3.4:88615701-88615720	None:intergenic	55.0%
	AGCAAGGCTGTGGCTATCAG+TGG	-	chr3.4:88616397-88616416	None:intergenic	55.0%
	ATCCACGTCCGAGCAAGAAG+GGG	+	chr3.4:88616366-88616385	MS.gene41654:CDS	55.0%
	CGGACGTGGATATAGCCAGT+TGG	-	chr3.4:88616357-88616376	None:intergenic	55.0%
	GGGTGAAACTGTGAAGGTGG+AGG	-	chr3.4:88615707-88615726	None:intergenic	55.0%
	GTGCCAGGCTGTGATAAGGT+AGG	+	chr3.4:88616608-88616627	MS.gene41654:intron	55.0%
	TGTTTGGATGAGCCCCCAAC+TGG	+	chr3.4:88616339-88616358	MS.gene41654:CDS	55.0%
	TGGCCTGACCCCTTCTTGCT+CGG	-	chr3.4:88616377-88616396	None:intergenic	60.0%
	CGTCCGAGCAAGAAGGGGTC+AGG	+	chr3.4:88616371-88616390	MS.gene41654:CDS	65.0%
	GACCCCTTCTTGCTCGGACG+TGG	-	chr3.4:88616371-88616390	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	88615623	88617627	88615623	ID=MS.gene41654
chr3.4	mRNA	88615623	88617627	88615623	ID=MS.gene41654.t1;Parent=MS.gene41654
chr3.4	exon	88615623	88615940	88615623	ID=MS.gene41654.t1.exon1;Parent=MS.gene41654.t1
chr3.4	CDS	88615623	88615940	88615623	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1
chr3.4	exon	88616252	88616422	88616252	ID=MS.gene41654.t1.exon2;Parent=MS.gene41654.t1
chr3.4	CDS	88616252	88616422	88616252	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1
chr3.4	exon	88616560	88616625	88616560	ID=MS.gene41654.t1.exon3;Parent=MS.gene41654.t1
chr3.4	CDS	88616560	88616625	88616560	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1
chr3.4	exon	88616766	88616834	88616766	ID=MS.gene41654.t1.exon4;Parent=MS.gene41654.t1
chr3.4	CDS	88616766	88616834	88616766	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1
chr3.4	exon	88616932	88617015	88616932	ID=MS.gene41654.t1.exon5;Parent=MS.gene41654.t1
chr3.4	CDS	88616932	88617015	88616932	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1
chr3.4	exon	88617111	88617305	88617111	ID=MS.gene41654.t1.exon6;Parent=MS.gene41654.t1
chr3.4	CDS	88617111	88617305	88617111	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1
chr3.4	exon	88617619	88617627	88617619	ID=MS.gene41654.t1.exon7;Parent=MS.gene41654.t1
chr3.4	CDS	88617619	88617627	88617619	ID=cds.MS.gene41654.t1;Parent=MS.gene41654.t1

Gene Sequence

>MS.gene41654

ATGGCAGCTTTTTCATATCAATACCATCCTTTCCTTGTTGACTCATCATTCATGCTCAACATGAACACTGCTTCTCCTCCTCCTCCACCTTCACAGTTTCACCCTCTTCATCAAGATATTATTAACTGTGTTGATCAACAAAGCTCCAAAGTTACTACCATAACTGAGAATGAACCTTCTTCTTTCACCAAAAACCTTAGTCCACAGTCTTCCATGGTTCTGGACAAGCTTGAAAATGGTGATGAGCAAGTTACTCAGAAAAATAGTCCTACGGAGAAGAAGAGGAGAACCAGAAACAATACTAACCCTCACTCTCATGTAATAATATTAATTGTTTCTGTTTCCATCTCCATCATATATATCACTGCTAGAAAAAACTAAAATTATCAATCAAAATTATAGACAAAAATATAAAATTCCTATGACAGAAGAATTAGAGATATGTTTAATGTTTTCCTTATTTTAAGAAAACTTGTGGATAATTTTTTTGGTAATGTATTTTTAGCACTTTTTTTATACTATGGTTTCATTAATTAAGCCATGCACACACACACACACATGTGTTTTGTTCCTGTTATGAATTGAGTTGATTATTTTGTACTGAATTTGTTAGTAATTAAATATGACAGGATAATGCAGCAGAAGGGAAAAACAAGAAACTGAAGAAAAACAAAGAAGATGAGAAGGCAAAAGCAGAGAAAAAAGATCAGAGAAAGTGTTTGGATGAGCCCCCAACTGGCTATATCCACGTCCGAGCAAGAAGGGGTCAGGCCACTGATAGCCACAGCCTTGCTGAAAGGGTATAGTACATTACTAGTATTTTCATTTCATGTGGGGTCCACTTGTATGATACAATTCCAAAAATGTCCCTTTTAGTTATCTTCATGTCTCATTCTGAATGTTGCAAATTGAATTAAATTGGATGAATTATATTCAGGTAAGAAGAGAGAAAATAAGTGAAAGGATGAAGAAGTTGCAGCAACTTGTGCCAGGCTGTGATAAGGTAGGTCAAGTAAAACCTTCATGACAGCACATATAGGCCCATGCAATTAAAAGTCTCAAAACTATACTGTTGAAAATAATATCTTTAATAATGAACATTCTATTTTTCATAAAATATAATTATTTATGGATTGTAACCAGGTAACTGGAAAGGCTCTTATGTTAGATGAAATAATCAATTATGTTCAGTCTTTACAGAATCAAGTAGAGGTAACATCAATATTCTCAAGCTGTTTTGTGGTTATTTATTTACTTAAAATGTATAAATATACTAATTAACAAGCTATATGCTCTCTATTTTTTTCAGTTCTTGTCAATGAAACTTACTTCTGTGAATCCTATGTTCTATGACATGGCAACAGACCTAGACACTCTTTTGGTTAGACAAGAGGTGAGTAAGAAAATTCAGTGATATATGATGATTCACATGTTAGGTCTAATAATGAAATTAACTAACTAACTTAGTATTTTTGAATGAATTATCAGAAATTAAATAACTTGGCATCACCATCAACATTATCATCTGTGTCACATTGCAATAGTCCTAAACAAGGCACAACTTTTGCTGATACATCAACCATGACTCCAACCAACATCTTCCAAGCTGCTAATGACTACCTTTTGGATACTTCAGCTTCAATTTTTCTACAAGGGCAAAGGTCATCAAATGTTGTCTCTGAGGTATAAAAACACAAATAACCAAAAAAATGATTTAATCCTAAAATGGATGTAATTATTCTTTTCTTATTCTGACAAAAAATAACATTTTCAGGATGGTAGTCATTTTTGGGAGGGACAAAAGTTTCTTCATTCACATGGATTCAACAGCAACTTATGTTCCTTTCATTAATTTAAAATTCACAGATGTGCATGCCCTACAAAACTACCAACATGTTTGTAAGCTCTCAAATCCTGTCTCTTATATTATATAATTTAATTTACTTAATAATACTTGGTTTCTAATTCTAATACTTATAATTTTTTTTTTCTTCAGGATTTTTAG

Protein sequence

>MS.gene41654.t1

MAAFSYQYHPFLVDSSFMLNMNTASPPPPPSQFHPLHQDIINCVDQQSSKVTTITENEPSSFTKNLSPQSSMVLDKLENGDEQVTQKNSPTEKKRRTRNNTNPHSHDNAAEGKNKKLKKNKEDEKAKAEKKDQRKCLDEPPTGYIHVRARRGQATDSHSLAERVRREKISERMKKLQQLVPGCDKVTGKALMLDEIINYVQSLQNQVEFLSMKLTSVNPMFYDMATDLDTLLVRQEKLNNLASPSTLSSVSHCNSPKQGTTFADTSTMTPTNIFQAANDYLLDTSASIFLQGQRSSNVVSEDF