Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42213.t1 | XP_003623813.1 | 74.1 | 417 | 36 | 3 | 125 | 472 | 78 | 491 | 1.60E-158 | 569.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42213.t1 | Q9C8S6 | 54.0 | 124 | 57 | 0 | 1 | 124 | 1 | 124 | 1.8e-33 | 145.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42213.t1 | G7L1G2 | 74.1 | 417 | 36 | 3 | 125 | 472 | 78 | 491 | 1.2e-158 | 569.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene42213 | 0.813966 | 1.96E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene42213 | 0.800993 | 1.11E-48 | -1.69E-46 |
| MS.gene053272 | MS.gene42213 | -0.816005 | 6.91E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene42213 | 0.809236 | 2.09E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene42213 | 0.809602 | 1.75E-50 | -1.69E-46 |
| MS.gene058781 | MS.gene42213 | 0.842684 | 2.22E-58 | -1.69E-46 |
| MS.gene058783 | MS.gene42213 | 0.820649 | 6.13E-53 | -1.69E-46 |
| MS.gene060935 | MS.gene42213 | 0.822104 | 2.83E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene42213 | 0.818776 | 1.64E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42213.t1 | MTR_7g075940 | 94.035 | 285 | 17 | 0 | 188 | 472 | 207 | 491 | 0.0 | 549 |
| MS.gene42213.t1 | MTR_7g075940 | 84.615 | 39 | 4 | 1 | 118 | 156 | 73 | 109 | 2.96e-11 | 65.9 |
| MS.gene42213.t1 | MTR_1g008950 | 66.400 | 125 | 42 | 0 | 1 | 125 | 1 | 125 | 3.57e-54 | 177 |
| MS.gene42213.t1 | MTR_8g032300 | 60.000 | 125 | 50 | 0 | 1 | 125 | 1 | 125 | 1.57e-51 | 171 |
| MS.gene42213.t1 | MTR_8g032320 | 57.600 | 125 | 53 | 0 | 1 | 125 | 1 | 125 | 2.42e-49 | 166 |
| MS.gene42213.t1 | MTR_5g079880 | 40.602 | 133 | 68 | 3 | 1 | 122 | 1 | 133 | 4.37e-30 | 114 |
| MS.gene42213.t1 | MTR_7g075960 | 92.453 | 53 | 4 | 0 | 25 | 77 | 1 | 53 | 1.47e-28 | 107 |
| MS.gene42213.t1 | MTR_5g079890 | 37.143 | 140 | 75 | 4 | 1 | 127 | 1 | 140 | 2.30e-25 | 101 |
| MS.gene42213.t1 | MTR_5g079890 | 36.486 | 148 | 81 | 4 | 1 | 135 | 1 | 148 | 6.20e-25 | 100 |
| MS.gene42213.t1 | MTR_8g030870 | 34.074 | 135 | 75 | 5 | 1 | 122 | 1 | 134 | 1.50e-14 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42213.t1 | AT4G00231 | 53.169 | 284 | 118 | 4 | 189 | 468 | 201 | 473 | 3.78e-90 | 283 |
| MS.gene42213.t1 | AT1G63220 | 54.032 | 124 | 57 | 0 | 1 | 124 | 1 | 124 | 1.31e-42 | 148 |
| MS.gene42213.t1 | AT1G63220 | 46.774 | 124 | 52 | 1 | 1 | 124 | 1 | 110 | 3.06e-32 | 120 |
| MS.gene42213.t1 | AT3G55470 | 39.098 | 133 | 70 | 4 | 1 | 122 | 1 | 133 | 3.03e-23 | 96.3 |
| MS.gene42213.t1 | AT3G55470 | 38.806 | 134 | 70 | 5 | 1 | 122 | 1 | 134 | 1.42e-21 | 91.7 |
| MS.gene42213.t1 | AT4G00467 | 33.083 | 133 | 78 | 3 | 1 | 122 | 1 | 133 | 3.22e-15 | 74.3 |
| MS.gene42213.t1 | AT4G00467 | 33.083 | 133 | 78 | 3 | 1 | 122 | 1 | 133 | 3.46e-15 | 74.7 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAACACTTGTTCTAAA+TGG | 0.190038 | 7.3:-29799811 | None:intergenic |
| GGAATATTCAAGAAATCTAT+TGG | 0.262223 | 7.3:+29804241 | MS.gene42213:CDS |
| CATTCTCAAGAAACATATTC+TGG | 0.280466 | 7.3:-29802110 | None:intergenic |
| ACCAGTTGATTCTCGACTTT+TGG | 0.292771 | 7.3:+29803078 | MS.gene42213:CDS |
| ATTCCTTTCCTGATTATTGC+TGG | 0.295665 | 7.3:-29804472 | None:intergenic |
| AGAGACATAGTTGCACTTAT+TGG | 0.310073 | 7.3:+29804562 | MS.gene42213:CDS |
| ATTACTGAAGTTGATGTTCT+TGG | 0.328366 | 7.3:+29804310 | MS.gene42213:CDS |
| GAATATTCAAGAAATCTATT+GGG | 0.346324 | 7.3:+29804242 | MS.gene42213:CDS |
| AGACCAAGTGCAGAAATTTC+AGG | 0.348807 | 7.3:-29804790 | None:intergenic |
| CATGTCCTTACAAGGGCTTT+AGG | 0.349168 | 7.3:+29804539 | MS.gene42213:CDS |
| GATGTTGCATCATTTGTTTA+TGG | 0.359521 | 7.3:+29804220 | MS.gene42213:CDS |
| TTGGCAATTGTGTGTATAGA+AGG | 0.366949 | 7.3:+29804581 | MS.gene42213:CDS |
| TGATTATTGCTGGAGGTTCA+AGG | 0.376770 | 7.3:-29804462 | None:intergenic |
| CGGAGATGTTACTATTTCTC+TGG | 0.385877 | 7.3:+29804198 | MS.gene42213:CDS |
| GTCATATGGCCTCATTGAGT+TGG | 0.394375 | 7.3:+29804420 | MS.gene42213:CDS |
| GTTGATATGTTATTGTCATA+TGG | 0.396469 | 7.3:+29804406 | MS.gene42213:CDS |
| GTTCTTGAACATGCAGTTAG+AGG | 0.397790 | 7.3:+29804265 | MS.gene42213:CDS |
| TCATATGGCCTCATTGAGTT+GGG | 0.401767 | 7.3:+29804421 | MS.gene42213:CDS |
| AATGGGGCATGTGGTGTGTT+AGG | 0.403361 | 7.3:+29804695 | MS.gene42213:CDS |
| TTGAACCTCCAGCAATAATC+AGG | 0.404931 | 7.3:+29804464 | MS.gene42213:CDS |
| TTAGAACAAGTGTTTGTTGA+TGG | 0.410957 | 7.3:+29799815 | MS.gene42213:CDS |
| TATACTAAGGATGACTTCCT+TGG | 0.418388 | 7.3:+29786812 | MS.gene42213:CDS |
| TTACGGGTTATGGATATTCC+TGG | 0.421957 | 7.3:+29804085 | MS.gene42213:CDS |
| CATGCAGTTAGAGGCAAGTC+TGG | 0.428607 | 7.3:+29804274 | MS.gene42213:CDS |
| AGCATGTACAAGATGAAATC+AGG | 0.434103 | 7.3:+29804605 | MS.gene42213:CDS |
| GTTATTCGTATTAGTGCAAA+AGG | 0.437294 | 7.3:+29786162 | MS.gene42213:CDS |
| ATACTAAGGATGACTTCCTT+GGG | 0.441937 | 7.3:+29786813 | MS.gene42213:CDS |
| GTGCCTGAAATTTCTGCACT+TGG | 0.443693 | 7.3:+29804787 | MS.gene42213:CDS |
| TAATCCTTACCTGAGAGAAT+GGG | 0.449263 | 7.3:+29804678 | MS.gene42213:CDS |
| AGATGTCCCTCAACATAGTA+AGG | 0.449798 | 7.3:-29804339 | None:intergenic |
| CTCAAAACCATGTCCTTACA+AGG | 0.456241 | 7.3:+29804531 | MS.gene42213:CDS |
| GTTCAGGATCTCATCCTCAT+TGG | 0.462694 | 7.3:+29786717 | MS.gene42213:CDS |
| ATAATCCTTACCTGAGAGAA+TGG | 0.466494 | 7.3:+29804677 | MS.gene42213:CDS |
| AGTTTCTGAATTGCAGCTGC+AGG | 0.466530 | 7.3:+29804753 | MS.gene42213:CDS |
| TACACTTCATCTGAGACTAA+TGG | 0.466893 | 7.3:+29786778 | MS.gene42213:CDS |
| CAGGCAATTGAGAGTCTTCA+AGG | 0.472548 | 7.3:-29804048 | None:intergenic |
| CTACTCCCTTACTATGTTGA+GGG | 0.473300 | 7.3:+29804333 | MS.gene42213:CDS |
| GTATCTTCTGCAAATCATAT+CGG | 0.474518 | 7.3:+29804156 | MS.gene42213:CDS |
| TAGAACAAGTGTTTGTTGAT+GGG | 0.479373 | 7.3:+29799816 | MS.gene42213:CDS |
| TCTCTCCTAAAGCCCTTGTA+AGG | 0.493388 | 7.3:-29804544 | None:intergenic |
| GCTACTCCCTTACTATGTTG+AGG | 0.495287 | 7.3:+29804332 | MS.gene42213:CDS |
| TACTGTTTGAAATTGTGAAA+AGG | 0.497480 | 7.3:-29786751 | None:intergenic |
| ACCACATGCCCCATTCTCTC+AGG | 0.508154 | 7.3:-29804687 | None:intergenic |
| AGAATCAACTGGTTTAACTA+AGG | 0.511410 | 7.3:-29803068 | None:intergenic |
| GTCTTGGTATCTTCACCGCC+AGG | 0.521162 | 7.3:-29804103 | None:intergenic |
| GCCAAAAGTCGAGAATCAAC+TGG | 0.523160 | 7.3:-29803079 | None:intergenic |
| CTAAAGCCCTTGTAAGGACA+TGG | 0.523837 | 7.3:-29804538 | None:intergenic |
| GGATGAAGATACATATACTA+AGG | 0.531224 | 7.3:+29786799 | MS.gene42213:CDS |
| CGATAAGTGAGGATCACATA+AGG | 0.534214 | 7.3:-29786408 | None:intergenic |
| GATGAAATCAGGAGTAGGAA+TGG | 0.534747 | 7.3:+29804616 | MS.gene42213:CDS |
| AAACTGATCATCTATTGTCT+TGG | 0.535911 | 7.3:-29804119 | None:intergenic |
| CCTTTCCTGATTATTGCTGG+AGG | 0.545513 | 7.3:-29804469 | None:intergenic |
| ATGCCCCATTCTCTCAGGTA+AGG | 0.547944 | 7.3:-29804682 | None:intergenic |
| CATAGAAGTAGTGCGGCTAA+AGG | 0.552777 | 7.3:+29786441 | MS.gene42213:CDS |
| AACTTCTTATAGTGTTGTGA+AGG | 0.553767 | 7.3:+29799850 | MS.gene42213:CDS |
| CTTCTCAGAAACCTATGTGC+TGG | 0.554353 | 7.3:+29802075 | MS.gene42213:CDS |
| GGGCAATGAGGAAAATCAAA+AGG | 0.555446 | 7.3:+29804729 | MS.gene42213:CDS |
| ATCAACTTCAGTAATGCCAC+TGG | 0.557884 | 7.3:-29804302 | None:intergenic |
| CTATAGTCAAAGATGCCTCA+TGG | 0.560161 | 7.3:+29786129 | None:intergenic |
| GGCAGTCTTTGAAAATTCTG+AGG | 0.563540 | 7.3:+29803099 | MS.gene42213:CDS |
| TGAAGGAGAAGAATTACTGC+GGG | 0.565150 | 7.3:+29799867 | MS.gene42213:CDS |
| TTACTGCGGGGAGGTTAAAG+TGG | 0.565620 | 7.3:+29799880 | MS.gene42213:CDS |
| TATAGTCAAAGATGCCTCAT+GGG | 0.565815 | 7.3:+29786130 | None:intergenic |
| TTCTGCACTTGGTCTACGAG+TGG | 0.575437 | 7.3:+29804798 | MS.gene42213:CDS |
| TTAGAATTTCACCAGCACAT+AGG | 0.583484 | 7.3:-29802086 | None:intergenic |
| TGGAAATTGATCAGAAAACT+CGG | 0.588086 | 7.3:+29804818 | MS.gene42213:CDS |
| GTTTCTGAATTGCAGCTGCA+GGG | 0.589710 | 7.3:+29804754 | MS.gene42213:CDS |
| GAATATGTTGGAGGGCAATG+AGG | 0.594983 | 7.3:+29804717 | MS.gene42213:CDS |
| GATCCTCACTTATCGATCAC+AGG | 0.597403 | 7.3:+29786416 | MS.gene42213:CDS |
| TTACTCACATTGCCTCCCCA+AGG | 0.599298 | 7.3:-29786829 | None:intergenic |
| CCTCCAGCAATAATCAGGAA+AGG | 0.599886 | 7.3:+29804469 | MS.gene42213:CDS |
| AATATTCAAGAAATCTATTG+GGG | 0.608850 | 7.3:+29804243 | MS.gene42213:CDS |
| GAATAAAACCCAACTCAATG+AGG | 0.614701 | 7.3:-29804429 | None:intergenic |
| GTGGTGTGTTAGGAATATGT+TGG | 0.615054 | 7.3:+29804705 | MS.gene42213:CDS |
| GTGTGTTAGGAATATGTTGG+AGG | 0.616384 | 7.3:+29804708 | MS.gene42213:CDS |
| ACCTGAGAGAATGGGGCATG+TGG | 0.618478 | 7.3:+29804686 | MS.gene42213:CDS |
| AATCCTTACCTGAGAGAATG+GGG | 0.625112 | 7.3:+29804679 | MS.gene42213:CDS |
| AATATGTTTCTTGAGAATGA+CGG | 0.631982 | 7.3:+29802114 | MS.gene42213:CDS |
| AGTCTTTGAAAATTCTGAGG+TGG | 0.637846 | 7.3:+29803102 | MS.gene42213:CDS |
| TACAAGATGAAATCAGGAGT+AGG | 0.639744 | 7.3:+29804611 | MS.gene42213:CDS |
| AAACATTCTGAGAATCAAGA+CGG | 0.647867 | 7.3:+29804496 | MS.gene42213:CDS |
| GATGTCCCTCAACATAGTAA+GGG | 0.650998 | 7.3:-29804338 | None:intergenic |
| GCTAAGCTTGTAAACGTGAG+TGG | 0.657403 | 7.3:+29804844 | MS.gene42213:CDS |
| TCAAAACCATGTCCTTACAA+GGG | 0.661079 | 7.3:+29804532 | MS.gene42213:CDS |
| TAAGGATGACTTCCTTGGGG+AGG | 0.669236 | 7.3:+29786817 | MS.gene42213:CDS |
| AGGAAGATCTCATTCCAATG+AGG | 0.671516 | 7.3:-29786731 | None:intergenic |
| TACTAAGGATGACTTCCTTG+GGG | 0.672162 | 7.3:+29786814 | MS.gene42213:CDS |
| GCTCCTGTGATCGATAAGTG+AGG | 0.674151 | 7.3:-29786419 | None:intergenic |
| TAGTGTGAGAGGTAATGCAG+AGG | 0.686152 | 7.3:+29804372 | MS.gene42213:CDS |
| GGCAAGTCTGGTCTTCCCAG+TGG | 0.687903 | 7.3:+29804286 | MS.gene42213:CDS |
| GGAGAAGAATTACTGCGGGG+AGG | 0.696027 | 7.3:+29799871 | MS.gene42213:CDS |
| ACAGGAGCATAGAAGTAGTG+CGG | 0.696113 | 7.3:+29786434 | MS.gene42213:CDS |
| TCAACTTCAGTAATGCCACT+GGG | 0.698559 | 7.3:-29804301 | None:intergenic |
| GTGAAGGAGAAGAATTACTG+CGG | 0.698719 | 7.3:+29799866 | MS.gene42213:CDS |
| TGTGTTAGGAATATGTTGGA+GGG | 0.700434 | 7.3:+29804709 | MS.gene42213:CDS |
| TGTGCTCATGATAGTGTGAG+AGG | 0.700764 | 7.3:+29804361 | MS.gene42213:CDS |
| CGGGTTATGGATATTCCTGG+CGG | 0.701302 | 7.3:+29804088 | MS.gene42213:CDS |
| GGTTTAACTAAGGAACACGA+TGG | 0.721182 | 7.3:-29803058 | None:intergenic |
| ATAACTTCAAGCATCCCATG+AGG | 0.740558 | 7.3:-29786144 | None:intergenic |
| GAAGGAGAAGAATTACTGCG+GGG | 0.779491 | 7.3:+29799868 | MS.gene42213:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 29786141 | 29804873 | 29786141 | ID=MS.gene42213 |
| chr7.3 | mRNA | 29786141 | 29804873 | 29786141 | ID=MS.gene42213.t1;Parent=MS.gene42213 |
| chr7.3 | exon | 29786141 | 29786207 | 29786141 | ID=MS.gene42213.t1.exon1;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29786141 | 29786207 | 29786141 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| chr7.3 | exon | 29786397 | 29786462 | 29786397 | ID=MS.gene42213.t1.exon2;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29786397 | 29786462 | 29786397 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| chr7.3 | exon | 29786717 | 29786843 | 29786717 | ID=MS.gene42213.t1.exon3;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29786717 | 29786843 | 29786717 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| chr7.3 | exon | 29799808 | 29799922 | 29799808 | ID=MS.gene42213.t1.exon4;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29799808 | 29799922 | 29799808 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| chr7.3 | exon | 29802075 | 29802166 | 29802075 | ID=MS.gene42213.t1.exon5;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29802075 | 29802166 | 29802075 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| chr7.3 | exon | 29803030 | 29803123 | 29803030 | ID=MS.gene42213.t1.exon6;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29803030 | 29803123 | 29803030 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| chr7.3 | exon | 29804016 | 29804873 | 29804016 | ID=MS.gene42213.t1.exon7;Parent=MS.gene42213.t1 |
| chr7.3 | CDS | 29804016 | 29804873 | 29804016 | ID=cds.MS.gene42213.t1;Parent=MS.gene42213.t1 |
| Gene Sequence |
| Protein sequence |
