Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42589.t1 | XP_003625072.1 | 97.9 | 94 | 2 | 0 | 1 | 94 | 22 | 115 | 9.20E-45 | 189.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42589.t1 | Q39096 | 59.7 | 67 | 23 | 2 | 1 | 63 | 22 | 88 | 1.6e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42589.t1 | Q2HRG8 | 97.9 | 94 | 2 | 0 | 1 | 94 | 22 | 115 | 6.7e-45 | 189.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene42589 | 0.806543 | 7.82E-50 | -1.69E-46 |
| MS.gene051676 | MS.gene42589 | 0.811481 | 6.85E-51 | -1.69E-46 |
| MS.gene052493 | MS.gene42589 | 0.811731 | 6.04E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene42589 | 0.812678 | 3.76E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene42589 | 0.812083 | 5.07E-51 | -1.69E-46 |
| MS.gene060259 | MS.gene42589 | 0.86319 | 2.97E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGGAGCAAAGAAATTTAC+TGG | 0.372256 | 7.1:-16659864 | MS.gene42589:CDS |
| GTCACCAGCTGCCACCATTC+CGG | 0.393504 | 7.1:+16660206 | None:intergenic |
| TGAAATGGTACCATGATTAC+TGG | 0.394842 | 7.1:-16660178 | MS.gene42589:CDS |
| AGCCTTCATCATCATGTTGC+TGG | 0.408811 | 7.1:+16660150 | None:intergenic |
| TTCTCCGGAATGGTGGCAGC+TGG | 0.463269 | 7.1:-16660210 | MS.gene42589:CDS |
| GCATCAAGATCAAACGCATC+AGG | 0.498917 | 7.1:+16660077 | None:intergenic |
| GCTGGCTAAGCCAGTAATCA+TGG | 0.519480 | 7.1:+16660168 | None:intergenic |
| AGCTGGTGACAACACTGAAA+TGG | 0.546239 | 7.1:-16660193 | MS.gene42589:CDS |
| GATGATGTAGATGAGTTTGA+CGG | 0.549584 | 7.1:-16660122 | MS.gene42589:CDS |
| AGCTGCATTTCGTTATGTCG+AGG | 0.550315 | 7.1:-16660237 | MS.gene42589:CDS |
| AGCCAGCAACATGATGATGA+AGG | 0.560493 | 7.1:-16660152 | MS.gene42589:CDS |
| AGAGTTTATCAAGTCTTCTG+AGG | 0.595138 | 7.1:-16660021 | MS.gene42589:CDS |
| ATCAAGTCTTCTGAGGCACA+AGG | 0.619098 | 7.1:-16660014 | MS.gene42589:intron |
| TGATGCGTTTGATCTTGATG+CGG | 0.644823 | 7.1:-16660075 | MS.gene42589:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTTTCCTCTGAAAAATAA+CGG | + | chr7.1:16660203-16660222 | None:intergenic | 25.0% |
| !! | TTTTTCAGAGGAAAGTTTTG+TGG | - | chr7.1:16660206-16660225 | MS.gene42589:CDS | 30.0% |
| !!! | AACATACCGTTATTTTTCAG+AGG | - | chr7.1:16660194-16660213 | MS.gene42589:CDS | 30.0% |
| AGAGTTTATCAAGTCTTCTG+AGG | - | chr7.1:16660074-16660093 | MS.gene42589:CDS | 35.0% | |
| CAAGGAGCAAAGAAATTTAC+TGG | - | chr7.1:16660231-16660250 | MS.gene42589:CDS | 35.0% | |
| GATGATGTAGATGAGTTTGA+CGG | - | chr7.1:16659973-16659992 | MS.gene42589:intron | 35.0% | |
| TGAAATGGTACCATGATTAC+TGG | - | chr7.1:16659917-16659936 | MS.gene42589:intron | 35.0% | |
| ! | TTATGTCGAGGATTTTTCTC+CGG | - | chr7.1:16659870-16659889 | MS.gene42589:CDS | 35.0% |
| TGATGCGTTTGATCTTGATG+CGG | - | chr7.1:16660020-16660039 | MS.gene42589:CDS | 40.0% | |
| AGCCTTCATCATCATGTTGC+TGG | + | chr7.1:16659948-16659967 | None:intergenic | 45.0% | |
| AGCTGGTGACAACACTGAAA+TGG | - | chr7.1:16659902-16659921 | MS.gene42589:intron | 45.0% | |
| GCATCAAGATCAAACGCATC+AGG | + | chr7.1:16660021-16660040 | None:intergenic | 45.0% | |
| ! | AGCCAGCAACATGATGATGA+AGG | - | chr7.1:16659943-16659962 | MS.gene42589:intron | 45.0% |
| ! | AGGATTTTTCTCCGGAATGG+TGG | - | chr7.1:16659878-16659897 | MS.gene42589:CDS | 45.0% |
| ! | TCGAGGATTTTTCTCCGGAA+TGG | - | chr7.1:16659875-16659894 | MS.gene42589:CDS | 45.0% |
| !! | ATCAAGTCTTCTGAGGCACA+AGG | - | chr7.1:16660081-16660100 | MS.gene42589:CDS | 45.0% |
| !! | GAGGAAAGTTTTGTGGAGCA+AGG | - | chr7.1:16660213-16660232 | MS.gene42589:CDS | 45.0% |
| GCTGGCTAAGCCAGTAATCA+TGG | + | chr7.1:16659930-16659949 | None:intergenic | 50.0% | |
| GTCACCAGCTGCCACCATTC+CGG | + | chr7.1:16659892-16659911 | None:intergenic | 60.0% | |
| TTCTCCGGAATGGTGGCAGC+TGG | - | chr7.1:16659885-16659904 | MS.gene42589:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 16659860 | 16660257 | 16659860 | ID=MS.gene42589 |
| chr7.1 | mRNA | 16659860 | 16660257 | 16659860 | ID=MS.gene42589.t1;Parent=MS.gene42589 |
| chr7.1 | exon | 16660015 | 16660257 | 16660015 | ID=MS.gene42589.t1.exon1;Parent=MS.gene42589.t1 |
| chr7.1 | CDS | 16660015 | 16660257 | 16660015 | ID=cds.MS.gene42589.t1;Parent=MS.gene42589.t1 |
| chr7.1 | exon | 16659860 | 16659903 | 16659860 | ID=MS.gene42589.t1.exon2;Parent=MS.gene42589.t1 |
| chr7.1 | CDS | 16659860 | 16659903 | 16659860 | ID=cds.MS.gene42589.t1;Parent=MS.gene42589.t1 |
| Gene Sequence |
| Protein sequence |
